Principles and Applications of Fluorescence Microscopy

Mechanisms of Living Organisms

This course is intended for Master’s students and international PhD students who expect to be using advanced live cell fluorescence microscopy for future studies.

Practical information

Institut Pasteur
March 2-13, 2020
November 30, 2019
In English


Credits for a Master 2 degree Université de Paris
Credits for a Master 2 degree Sorbonne Université
Doctoral School Module
Institut Pasteur Diploma

The use of fluorescence microscopy (wide field, confocal, multiphoton, and now superresolution) in combination with genetically encoded fluorescence probes comprise a powerful set of scientific tools to study live cells. However, surprising little practical and theoretical training in such methods exists within standard curricula, particular at the early stage of training (Masters or Doctorate level). This course offers to cover basic optics principles necessary to understand the origin of microscope resolution and design. Participants will get hands-on experience implementing simple optical configurations to illustrate these fundamental principles.  Subsequently, participants will perform experiments on state-of-the-art imaging equipment provided by microscope vendors.

Lectures will cover, in depth, the principle behind traditional high resolution imaging methods such as confocal, multi-photon, and the recently developed super-resolutions methods. Students will also learn about fundamental properties of synthetic and genetically encoded fluorescence indicators used for cellular morphology imaging and signaling recording. Finally guest lectures will demonstrate applications of some of the discussed methodologies.

The committee of the course will evaluate applications.


More informations


Jost Enninga
Unité de Dynamique des interactions hôte-pathogène
Institut Pasteur
Jean-Yves Tinevez
Hub d'Analyse d'images
Institut Pasteur
Gael Moneron
Unité d'Imagerie dynamique du neurone
Institut Pasteur

Head(s) of laboratory practicals

Florian Ruckerl

Unité d'Imagerie dynamique du neurone

Institut Pasteur

2 weeks
Members of the Course committee

J. Enninga (Institut Pasteur),

S. Louvet (Sorbonne Université),

S. Malot (Institut Pasteur),

G. Moneron (Institut Pasteur),

V. Ponticelli (Institut Pasteur),

F. Ruckerl (Institut Pasteur),

M. Sala (Institut Pasteur),

J.-Y. Tinevez (Institut Pasteur),

J-M. Verbavatz (Université de Paris),

H. Waxin (Institut Pasteur).

Application - specific conditions

only 1 letter of recommendation is required.

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