This two-week course is intended for medical doctors, veterinarians, directors of clinical mycobacteriology laboratories, pharmacists and research scientists wishing to acquire an up-to-date knowledge on tuberculosis, molecular methods for diagnosis and drug susceptibility testing, new drugs and treatment regimens as well as molecular and traditional epidemiology.
This full-time course consists of lectures by international scientists and a practical training workshop. The language for lectures and the practical training is English, but help for French or Spanish speakers will be provided during the practical training.
Lectures will cover: the history of tuberculosis; global epidemiology of tuberculosis (TB); molecular epidemiology and evolution of Mycobacterium tuberculosis strains; immune responses during tuberculosis infection and disease; TB vaccines; HIV co-infection; diabetes and TB; pediatric TB, mechanisms of antibiotic resistance; diagnosis and treatment of multidrug-resistant (MDR) and extensive drug resistant (XDR) TB; concepts and applications of rapid diagnosis and drug susceptibility tests (DST) in different settings; evaluation of the different methods using predictive values according to TB prevalence; whole genome sequencing and non-tuberculosis mycobacteria. There will also be an introduction to modeling of transmission and the effect of interventions, the use of computer assisted diagnosis of chest Xrays, and a session with difficult case studies.
The practical workshop will provide training for rapid diagnosis of TB and DST using nucleic acid-based technologies: GenoType® MTBDR plus and MTBDRsl (HAIN Lifescience GmbH, Germany), and the GeneXpert MTB/RIF test (Cepheid, Sunnyvale, CA, USA). Antibiotic gene targets will be sequenced to identify antibiotic resistance, with bioinformatics sessions to understand how to interpret the sequences. There will also be an introduction to Whole Genome Sequencing and the use of software for detecting resistance-associated mutations in whole genome sequence data. MIRU/VNTR and WGS analysis will be compared for use in molecular epidemiology and contact tracing.
Roland BROSCH Olivier NEYROLLES
Theodore COHEN Stefan NIEMANN
Tom DECROO Jean Louis HERRMANN
Christophe DELACOURT Catherine PIERRE-AUDIGIER
Francis DROBNIEWSKI Indira SOUNDIRAM
Brigitte GICQUEL Steffen STENGER
Philippe GLAZIOU Howard TAKIFF
Véronique JOLY Arnaud TREBUCQ
Qingyun LIU Nicolas VEZIRIS
Mycobacterial Genetics unit
Mycobacterial Genetics Unit
B. Gicquel (Institut Pasteur)
S. Malot (Institut Pasteur)
C. Pierre-Audigier (Institut Pasteur)
V. Ponticelli (Institut Pasteur)
M. Sala (Institut Pasteur)
H. Takiff (Institut Pasteur)
H. Waxin (Institut Pasteur)