Researchers at the Institut Pasteur and CNRS have shown for the first time that certain viruses are capable of forming complex biofilm-like assemblies, similar to bacterial biofilms. These extracellular infectious structures may protect viruses from the immune system and enable them to spread efficiently from cell to cell. "Viral biofilms" would appear to be a major mechanism of propagation for certain viruses. They are therefore emerging as new and particularly attractive therapeutic targets.
Paris, december 21, 2009
The HTLV-1 virus (human T-cell leukemia virus type 1) was the first human retrovirus to be isolated, in 1980, three years prior to the discovery of HIV, the retrovirus that causes AIDS. It infects 15 to 20 million people worldwide and causes various diseases, ranging from adult T-cell leukemia/lymphoma to forms of neuromyelopathy (tropical spastic paraparesis) or other chronic inflammatory syndromes, such as infectious dermatitis, uveitis and myositis. The dissemination of HTLV-1 was known to require infected cells and cell-cell contacts, but the transmission mechanism itself was still a mystery.
In the biofilm – an effective protective and adhesive barrier – HTLV-1 is far more easily transmitted than in its free, isolated state. By removing the viral biofilm from the surface of the infected cells, researchers achieved an 80% reduction in infection rates, thus underlining the importance of this transmission mode for HTLV-1.
In bacteria, the existence of biofilms has been known for many years. They form the dental plaque on the enamel surface of teeth and are also found in industrial systems and in our own intestinal flora. When they colonize medical implants, such as prosthesis or catheters, they can cause repeated infection. For these reasons, bacterial biofilms have been the focus of intensive research in the aim to limit their development and render them responsive to anti-bacterial treatment.
Scientists are currently seeking to characterize the mechanisms of viral biofilm generation, and to determine whether viruses other than HTLV-1 form this kind of structure. For viruses forming biofilms, it would be useful to define new anti-viral therapeutic strategies, which would target, not only the virus itself, but the formation of these viral biofilms.
Image : HTLV-1 biofilm (orange) at the cell surface. // Institut Pasteur/MI Thoulouze.
Biofilm-like extracellular viral assemblies mediate HTLV-1 cell-to-cell transmission at virological synapses, Nature Medicine, advanced online publication December 20, 2009.
Ana-Monica Pais-Correia (1), Martin Sachse (2), Stéphanie Guadagnini (2), Valentina Robbiati (1), Rémi Lasserre (1), Antoine Gessain (3), Olivier Gout (4), Andrés Alcover (1) and Maria-Isabel Thoulouze (1)
(1) Institut Pasteur, Lymphocyte Cell Biology Unit; CNRS, URA 1961, F-75015, Paris, France.
(2) Institut Pasteur, Ultrastructural Microscopy Platform, Imagopole, F-75015, Paris, France.
(3) Institut Pasteur, Oncogenic Virus Epidemiology and Physiopathology Unit; CNRS URA 1930, F-75015, Paris, France.
(4) Fondation A. Rothschild, Neurology Department, F-75019, Paris, France.
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