Light shed on the coordination of neural stem cell activation

Press release

In all adult vertebrates, neural stem cells can be recruited to produce new neurons in the brain. However, little is known about these so-called "activation" processes.
Scientists at the Institut Pasteur, CNRS, and Tel Aviv University working in collaboration with the École Polytechnique and INRAE have successfully performed 3D visualization and spatial and temporal distribution analysis of neural stem cell activation in the adult brain of a zebrafish vertebrate model.
Their findings demonstrate for the first time that activation events for these cells are coordinated in time and space. In particular, these results may help improve our understanding of regulation processes triggered during brain tumor formation. These findings are published in the April 5, 2021 issue of the journal Cell Stem Cell.

Stem cells, which are found in many adult organs in vertebrates including humans, are capable of proliferating and differentiating to generate new functional cells. For instance, stem cells in the brain (neural stem cells) produce new neurons in adulthood. Most of the time, neural stem cells are in a dormant state known as "quiescence". In order to produce neurons, they must therefore first activate and then divide. This activation stage is crucial: it is a prerequisite for stem cell recruitment and is also critical to their survival (cells that activate excessively become exhausted quickly) and to the position and type of neurons formed.

Within their niche, neural stem cells activate and return to a dormant state in random order and asynchronously, suggesting that these events may be coordinated at cell population level. Scientists in the Zebrafish Neurogenetics Unit[1] at the Institut Pasteur chose zebrafish to test this hypothesis, since zebrafish adult brains contain large quantities of neural stem cells, otherwise similar to mammalian stem cells. Through intravital (non-invasive) two-photon imaging of adult fish, they were able to film stem cells in their niche for several weeks and study the activation pattern of each cell in relation to neighboring cells in real time. Spatial statistical analysis and modeling performed both in real time and in long-term computational simulations demonstrated the existence of inhibitory interactions generated by activated cells, delaying the activation of other adjacent stem cells by several days. By administering a pharmacological molecule in vivo, the scientists also identified the molecular pathway involved, which is known as the Notch signaling pathway. Finally, they demonstrated that these interactions enabled steady neuronal production in time and space.

"This is the first real-time and long-term imaging of an entire neural stem cell population in the brain of an adult vertebrate. These findings demonstrate for the first time that neural stem cell activation events in the vertebrate brain are coordinated in time and space within the niche," commented Laure Bally-Cuif, CNRS scientist, lead author of the study, and Head of the Zebrafish Neurogenetics Unit1 at the Institut Pasteur. Unexpectedly, this study has also demonstrated that the stem cells themselves are involved in this coordination. Therefore, this research has prompted the emergence of a new concept whereby stem cell populations self-organize as a dynamic system enabling spatiotemporal coordination of the behavior of each individual cell.

Such regulation may occur in tumor masses containing cancerous stem cells, in which stem cells in various states of quiescence or activation have been observed. It is also likely that such regulation takes place in the stem cell populations of other adult organs where stem cells are found in compact niches, such as epithelia.

Intravital imaging of zebrafish neural stem cells over 23 days :

This animation shows stem cells covering one of the cerebral hemispheres of an adult fish. The stem cells were monitored for 23 days to analyze their positions, activation events, and differentiation. The fish were anesthetized and filmed using a two-photon microscope every 3 days.
The dots show the center of each cell and the arrows show dividing stem cells.
© Nicolas Dray, Laure Mancini and Laure Bally-Cuif, Institut Pasteur

Cellules souches et neurones (c) Institut Pasteur

Left: Neural stem cells covering both cerebral hemispheres of an adult zebrafish. Center: Magnification enabling identification of stem cells that are quiescent (green only), activated (green and magenta), and in the process of differentiation (magenta only). Right: Neurons generated by these stem cells during adulthood.
© Nicolas Dray, Laure Mancini and Laure Bally-Cuif, Institut Pasteur


[1] Department of Developmental and Stem Cell Biology (CNRS/Institut Pasteur)



Dynamic spatiotemporal coordination of neural stem cell fate decisions occurs through local feedback in the adult vertebrate brain, Cell Stem Cell, April 5th, 2021

Nicolas Dray1,*,$, Laure Mancini1,2,*, Udi Binshtok3,*, Felix Cheysson4,5,6,*, Willy Supatto7, Pierre Mahou7, Sébastien Bedu1, Sara Ortica1, Emmanuel Than-Trong1,£, Monika Krecsmarik1, Sébastien Herbert8,9, Jean-Baptiste Masson10, Jean-Yves Tinevez9, Gabriel Lang4, Emmanuel Beaurepaire7, David Sprinzak3,$, Laure Bally-Cuif1,$

1Zebrafish Neurogenetics Unit, Institut Pasteur, UMR3738, CNRS, Team supported by the Ligue Nationale Contre le Cancer, Paris (75015), France

2Sorbonne Université, Collège doctoral, F-75005 Paris, France

3School of Neurobiology, Biochemistry, and Biophysics, The George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv (69978), Israel  

4Université Paris-Saclay, AgroParisTech, INRAE, UMR MIA-Paris, Paris (75005), France

5Epidemiology and Modeling of bacterial Evasion to Antibacterials Unit (EMEA), Institut Pasteur, Paris (75015), France

6Anti-infective Evasion and Pharmacoepidemiology team, Centre for Epidemiology and Public Health (CESP), INSERM/UVSQ, France

7Laboratory for Optics and Biosciences, Ecole Polytechnique, CNRS, INSERM, IP Paris, Palaiseau (91128), France

8Department of Developmental and Stem Cell Biology, Institut Pasteur, Paris (75015), France

9Image Analysis Hub, Institut Pasteur, Paris (75015), France

10Department of Neuroscience and Department of Computational Biology, Institut Pasteur, Paris (75015), France

£Current address: Laboratoire des maladies neurodégénératives, Institut François Jacob, MIRCen, CEA/CNRS UMR 9199, Fontenay-aux-Roses, France

*Equal contribution

$Co-corresponding authors


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