Research / Scientific departments / Structural Biology and Chemistry / Units and Groups / Biophysics of Macromolecules and their interactions
logo PFBMI english nov 2010

The Centre of Biophysics of Macromolecules and their Interactions (french acronym: PFBMI) federates an ensemble of state-of-the-art instrumentation for the in vitro molecular-scale characterization of biological macromolecules, together with well established technical and methodological expertise. The mission of the PFBMI is to provide an added value to a great variety of biological projects,allowing to characterize the intrinsic properties of macromolecules and their assemblies (shape, folding, stability auto-association,and of the interactions in which they are involved (stoichiometry,thermodynamic and kinetic parameters, ...).


SOME OF THE BIOPHYSICAL PROPERTIES THAT CAN BE ADRESSEd, AND RELEVANT TECHNOLOGIES

Size and shape of macromolecules Stoichiometry and assembly of macromolecules
Secondary and tertiary structures of macromolecules Thermodynamical stability of macromolecules
Kinetic and thermodynamical properties of interactions  

TECHNOLOGIES  AVAILABLE AT THE PFBMI 

Analytical Ultracentrifugation Circular dichroism
Dynamic light scattering (DLS) Fluorescence spectroscopy
Infrared spectroscopy Microcalorimetry  (DSC and ITC)
Static light scattering and viscosimetry Surface Plasmon Resonance (SPR)
Dual polarization interferometry
 

Group leader : Patrick ENGLAND : patrick.england@pasteur.fr
Secretary : Jocelyne FRAYSSE : jocelyne.fraysse@pasteur.fr

Engineers :
Sylviane HOOS : sylviane.hoos@pasteur.fr
Bertrand RAYNAL : bertrand.raynal@pasteur.fr
Bruno BARON : bruno.baron@pasteur.fr