Job posting and tenders / Post-doctoral Positions

Offres de stages postdoctoraux à l'Institut Pasteur

 

10/07/2013 - POSTDOCTORAL POSITION OPEN AT THE INSTITUT PASTEUR, PARIS, FRANCE

 
Research Program :
Innate Control of HIV-1 Transmission at the Mucosal Level
 
Our team is working on the control of HIV-1 transmission in the female genital tract including the materno-fetal interface in human. We are particulary interested in the role of the innate immunity in the inhibition of HIV-1 infection.
 
A research opportunity is available in our team to study the role of the activation of Toll-Like Receptors (TLRs) and inflammation in the protection of HIV-1 transmission.
 
The applicant should have a Ph.D. degree and an experience in cellular virology and immunology. An expertise in mucosal innate immune responses will be particulary appreciated.
Applicants are invited to submit their CV, a brief statement of research interests and 3 reference letters to :
 
Dr Elisabeth Menu
INSTITUT PASTEUR
Unité de Régulation des Infections Rétrovirales
28 rue du Dr Roux
75724 Paris cedex 15
Tel : + 33 1 44 38 93 32


 


07/02/2013 - Postdoc position in Immunology at the Institut Pasteur (Mouquet lab)

The Laboratory of Humoral Response to Pathogens in the Immunology Department at the Institut Pasteur – Paris has a postdoctoral position opening for the Fall 2013. The lab works on exciting projects aiming to understand the molecular basis of virus-specific B-cell responses, with a focus on how antibodies participate in controlling human viral infections.
 
Projects outline: Our understanding of pathogen-specific B-cell responses has increased substantially in recent years with the advent ofmodern technologies that allow the generation of monoclonal antibodies derived from single B-cells. The power of these new technologies is highlighted by the breakthrough discoveries that have been made in the antiviral responses to rapidly changing pathogens such as Human Immunodeficiency Virus (HIV) and Influenza virus. Our research group aims to study the human antibody responses to viruses such as HIV by generating and characterizing envelope-specific antibodies from infected patients using an efficient method to clone and express immunoglobulins from single human B cells. These recombinant antibodies represent unique “fingerprints” for each B-cell clone and when characterized on a molecular level, provide crucial information about the antigen-specific humoral response to a given pathogen. In addition to the antibodies’ potential therapeutic interest, their characterization will lead to a better understanding of human antibody responses to pathogens, and might uncover candidate immunogens for vaccine development.
 
Candidate requirements: Applicants should have a strong background in Human B-cell Biology and Molecular Immunology, be highly motivated to work in a competitive field, and be willing to tackle intellectual/technical challenges. Technical experience in Molecular Biology and Biochemistry is highly recommended.
 
To apply: Applicants should send their CV, a motivation letter and 3 references to hmouquet@rockefeller.edu.
 
 
Research Keywords: Immunology, Adaptive Immunity, Viruses, B-lymphocytes, Antibodies, and HIV.



 16/01/2013 - Identification of novel regulators of senescence

 

Institut Pasteur Unit Oncogenesis and Nuclear Organization, INSERM U993

(Director : Anne DEJEAN ; Principal Investigator : Oliver BISCHOF)

Institut Pasteur, Paris.

Applications are invited to apply for a postdoctoral research position within the Oncogenesis and Nuclear Organization Unit at the Pasteur Institut in Paris. We are looking for a talented and motivated post-doctoral student interested in joining our team. The successful candidate will have the opportunity to work on one of several open projects involving the identification and characterization of the senescence phenotype with an emphasis on chromatin structurefunction and non-coding RNAs.

Candidates should be highly motivated, committed and have clear career goals.

Experience in senescence and cancer research would be an asset but are not crucial. A strong background in molecular and cellular biology techniques with a special emphasis on chromatin structure-function analysis and noncoding RNAs are essential. Moreoever, the candidate should have had significant exposure to the bioinformatic analysis of NGS data and should be familiar with culturing of primary cells, retroviral and lentiviral infections and sh/siRNA-mediated silencing. All applicants should have a Ph.D. degree in life sciences or equivalent experience and minimum one first-author publication published or in press in peerreviewed international journals. Institut Pasteur provides an excellent research infrastructure including many core facilities and support structures. For informal enquiries regarding the posititon, please, contact Dr. Oliver BISCHOF at obischof@pasteur.fr or +33140613307.

Please, send CV, letter of motivation and name/address of 3 referees to Dr. Oliver BISCHOF (obischof@pasteur.fr). or by regular mail to D. Oliver BISCHOF Institut Pasteur, Unité d'Organisation Nucleaire et Oncogenese (ONO), INSERM U993, Bat. Andre Lwoff, 28, rue du Dr Roux, 75724 Paris cedex 15, France.

Closing date : March 2013

Interviews: April 2013.

Recent Publications :

1. A New Player in PML-mediated Cellular Senescence: TBX2 Gets into the Loop (2012). Martin N, Dejean A, Bischof O. Med Sci (Paris). 2012 Mar;28(3):248-50.

2. Benhamed, M., Herbig, U. Dejean, A. and Bischof, O. (2012). Senescence is an Endogenous Trigger for MicroRNA-Mediated Transcriptional Gene Silencing in Human Cells. Nat. Cell Biol. 26;14(3):266-75. doi: 10.1038/ncb2443.

3. Nadine Martin, Moussa Benhamed, Karim Nacerddine, Maud Demarque, Maarten van Lohuizen, Anne Dejean and Oliver Bischof. (2011). Physical and Functional Interaction between PML and TBX2 in Cellular Senescence EMBO J. 14;31(1):95-109.

4. Martin, N., Schwamborn, K., Schreiber, V., Werner, A., Guillier, C., Zhang, X.D., Bischof, O., Seeler, J.S., Dejean, A. (2009). PARP-1 transcriptional activity is regulated by sumoylation upon heat shock. EMBO J. 22:3534-48.

5. Bischof, O., Pineau, P., and Dejean A. (2009). A Re-View of Cellular Senescence : Friend or Foe in Tumor Suppression. Médicine et Sciences, 25 :153-60.

6. Carter, S., Bischof, O., Dejean, A. and Vousden, K. (2007). C-terminal modifications regulate MDM2 dissociation and nuclear export of p53. Nat. Cell Biol., 9: 428-435.

7. Bischof, O. and Dejean, A. (2007). SUMO is Growing Senescent. Cell Cycle 6, 677-681.

8. Kumar, P.*, Bischof, O.*, Purbey, P.K., Notani, D., Urlaub, H., Dejean, A. and Galande, S. (2007).
Functional Interaction between PML and SATB1 Regulates Chromatin Loop Architecture and Transcription of the MHC class I Locus. (* Co-first authors). Nat. Cell Biol., 9:45-56.

9. Seeler J.S., Bischof O., Nacerddine K. and Dejean A. (2007). SUMO, the three Rs and Cancer.Acute Promyelocytic Leukemia: Mechanisms and Targets. Curr. Top. Microbiol. Immunol., 313:49-71.

10. Bischof, O., Schwamborn, K., Martin, N., Werner, A., Sustmann, C., Grosschedl, R. and Dejean, A. (2006). The E3 SUMO Ligase PIASy is a Novel Regulator of Cellular Senescence and Apoptosis. Mol Cell 22, 783-794.


18/12/2012 - 2-years post-doctoral position in Molecular and Cellular Virology

 

Description

A 2-years post-doctoral position is available at Institut Pasteur, in the group « Arboviral virulence factors and toxins », which is part of the Structural Virology unit. The position is financed in the context of a collaborative program between Institut Pasteur and Institut Mérieux.

 

The aim of the project is to further define the contribution of the dengue virus (DENV) nonstructural protein NS1 to viral pathogenesis. NS1 is a multifunctional protein that localizes to replication factories inside infected cells and is secreted in the extracellular fluid as a soluble hexameric particle (NS1hex). We recently reported that secreted NS1hex is an atypical high density lipoprotein that could possibly interfere with the vascular system during the course of infection (Gutsche et al., PNAS 2011). We also found that DENV NS1hex associates to host proteins during its circulation in the extracellular fluid. We propose to further characterize the nature of these complexes by different approaches (in vitro reconstitution, immunoassays, mass spectrometry, electron microscopy). The role of these complexes will be studied in relevant target cells such as hepatocytes and their prognostic value will be assessed in human patients, in collaboration with the Institut Pasteur International Network.

 

 

Qualification

The applicant should have a PhD with at least 3 years of post-doctoral experience in the field of virology/infectious diseases, and a strong background in biochemistry, molecular and cellular biology. A specific expertise on membrane proteins would be particularly appreciated. Good written and oral communication skills in english are required. The position is expected to start February 1st, 2013 at the latest.

 

Salary will depend on experience and past achievements.

 

 

Application

Applications should include a CV, a summary of previous research activities (2-3 pages) and 2 references (just include name and contact information), to be addressed by e-mail before January 10th, 2013 to

Marie Flamand (marie.flamand@pasteur.fr)


14/12/2012 - CRBIP (Biological Resources Center of Institut Pasteur) is looking for a project manager .

CRBIP is in charge of the valorisation of biological resources of the Institut Pasteur which are available in the collections. CRBIP is involved in national and international projects in order to facilitate access to biological resources of high quality and help in the creation of BRC networks. CRBIP is a member of the team management for these different projects.

Activities :

-     operational management of national and European projects 
-     business plan creation and follow-up of WP regarding MIRRI project and different CRBIP’s projects 
-     participation to national and international coordination
-     writing scientific reports of projects
-     follow-up of financial reports of projects
-     communications (newspapers relations and public relations) bounded to projects : news, information, proposal on communication possibilities
-     participation to national and European meetings 

Skills :

-          Very good knowledge in English (read, written, spoken)
-          Fair knowledge of pack office
-          Experience in project management
-          Good level in writing processes and scientific information
-          Team working
-          Diplomatic behaviour and listening ability
-          Initiatives capacity, reactivity and  adaptability are demanded

Job descriptions :

 -     Post doc
-      Full time
-      Managed by CRBIP’s head office.
-      5 years experience in the same position
-      Position opened in January 2013 for 2 years.
 

 


 

15/10/2012 - Microbes and Host Barriers Group, Institut Pasteur, Inserm, Paris, France

A funded postdoctoral position is available in the "Microbes and Host Barriers group" directed by Marc Lecuit at the Institut Pasteur in Paris.

The project focuses on Chikungunya virus (CHIKV), a recently re-emerged arbovirus transmitted to human by mosquito bite that belongs to the Alphavirus genus. It was responsible in 2006 for a massive outbreak, first in India and the islands of the Indian Ocean including the island of La Réunion, causing more than one million of cases of CHIKV infection. CHIKV is now regarded as the arbovirus most likely to spread globally, given the wide distribution of its mosquito vector. CHIKV induces a relatively mild disease in human, characterized by fever, arthralgia, myalgia and rash. Chronic and relapsing arthralgia is observed in a significant proportion of patients. Cases of severe CHIKV infection with central nervous system involvement have also been described, particularly in neonates born to viremic mothers. Neither vaccine nor therapy against CHIKV is commercially available.

We first developed an animal model to study the pathophysiology of infection, and its cell and tissue tropisms, and the resulting host responses. We now pursue our studies on CHIKV by focusing on the cell biology of this poorly studied Alphavirus.

The objective of the project is to identify the cellular genes involved in CHIKV replication. Genome-wide loss-of-function screens using small interfering RNA (siRNA) libraries and automation technologies have been conducted to discover host factors participating in the lifecycle of several viruses. We have followed a similar functional genome-wide RNAi screen approach to identify the cellular genes required for CHIKV entry and replication as well as those involved in the inhibition or the promotion of viral replication in human cells, in collaboration with Thomas Meyer (Max Planck Institute, Berlin, Germany). The project will consist in investigating in detail the role of genes identified by the whole genome screen. Once their role confirmed by independent experiments with individual siRNAs, their contribution to the infection process will be deciphered: their role in CHIKV infection will be analyzed by biochemical and molecular approaches to reveal their molecular mechanisms of actions. Interactions between cellular and viral gene products implicated in CHIKV replication will be characterized and analyzed using high-resolution imaging. The impact of the identified host gene products on CHIKV infection will be assessed in vivo in mouse models.

This study of the cell biology of this recently re-emerged virus will not only contribute to a better understanding of the basic biology of this virus, but will also lead to the identification of novel targets for the development of new antiviral drugs.

Candidates profile:

PhD, virology, cell biology.
Skills in molecular biology and biochemistry. Experience in in vitro culture and microscopy wouldbe appreciated.
Applicants should ideally possess a strong background in molecular biology, biochemistry and virology, be highly motivated and show an ability to work within a team. Our group enjoys numerous collaborations with researchers on campus and beyond. The Institut Pasteur campus hosts expert teams in cell biology, virology, molecular biology and microbial pathogenesis, as well as cutting-edge technical platforms, all of which will be essential for the success of the project.

 

 Website: http://www.pasteur.fr/research/mbh

Please send your application letter and a copy of your CV to marc.lecuit@pasteur.fr or therese.couderc@pasteur.fr


26/09/2012 - Membrane Traffic and Cell Division Laboratory

Institut PASTEUR, Paris, FRANCE

A funded postdoctoral position is currently available in the "Membrane Traffic and Cell Division laboratory" of Dr. Echard at the Institut Pasteur in Paris. This position has no nationality restriction and the working language is English.

Our research focuses on the role of membrane trafficking in lipid and cytoskeleton remodeling at each step of animal cell division, under normal and pathological conditions.  We are particularly interested in cytokinesis, the final step of cell division leading to the physical separation of the daughter cells. Using cells, high-content RNAi-based screens, state-of-the-art live-cell imaging and advanced microscopy techniques, we are exploring the novel and promising interfac between cytokinesis and membrane traffic at the cell and tissue level. Our recent work revealed unexpected connections between the human Lowe syndrome, endocytosis, cytoskeleton and cell division.

We are seeking a highly motivated candidate for joining our dynamic and international team with:
- a PhD and 0-3 years of relevant postdoctoral experience
- a strong track-record of publications in peer-reviewed journals
- validated experience in cell biology, RNAi-based screens, mouse models, lipid biology and/or cell imaging.

Please send your application as a single PDF document containing a cover letter, a concise summary of previous research, a Curriculum Vitae, a publication list and contact details for at least 2 references to arnaud.echard@pasteur.fr

For further information, please contact arnaud.echard@pasteur.fr and visit our website: http://www.pasteur.fr/ip/easysite/pasteur/en/research/scientific-departments/cell-biology-andinfection/

Selected Recent Publications:

An ARF6/Rab35 GTPase Cascade for Endocytic Recycling and Successful Cytokinesis.
Chesneau L, Dambournet D, Machicoane M, Kouranti I, Fukuda M, Goud B, Echard A.
Current Biology: 22, 147-53 (2012)

Rab35 GTPase and OCRL phosphatase remodel lipids and F-actin for successful cytokinesis.
Dambournet D., Machicoane M., Chesneau L., Rocancourt M., Formstecher E., Salomon R., Goud B. and Echard A.
Nature Cell Biology: 13, 981-8 (2011)

Rab and actomyosin-dependent fission of transport vesicles at the Golgi complex.
Miserey-Lenkei S, Chalancon G, Bardin S, Formstecher E, Goud B, Echard A.
Nature Cell Biology: 12, 645-54 (2010)


11/09/2012 Postdoctoral position in Virology Pasteur Institute

Description:
A 48 month postdoctoral position is available starting December 2012 to join a newly established research program within the Department of Virology of the Pasteur Institute, Paris.

Project outline:
Very early upon virus infection of mammalian cells, many anti-viral genes, collectively called as "viral stress-inducible genes' are activated directly by pathogen-associated molecular patterns (PAMPs) without the need of Interferon (IFN) signaling. The project aims at understanding the molecular mechanisms and the dynamics of this IFN-independent pathway in the context of infection by medically relevant viruses. A better understanding of this arm of the innate immune response should shed some light on the control of virus replication by the host cell, the cell tropism of the virus and the development of disease.

Key words:
dynamics of virus-host interaction, innate immunity, viral stress-inducible genes.

Candidate requirements:
Candidates must hold a PhD degree in Biological Sciences since less than 2 years. They must have experience in the domain of mammalian virology, innate immunity and cell biology. Experience in live cell microscopy will be advantageous. Candidates should have strong communication and organization skills and willing to working independently. French language is not required.

To apply:
Applicants should send a cover letter and a C.V (including a list of publications as well as names and contact information for up to 3 academic references)to Nolwenn Jouvenet (nolwennjouvenet@gmail.com).