This five-week intensive laboratory and lecture course presents advanced studies on the main themes in cellular and molecular immunology.
The course comprises:
LABORATORY PRACTICAL COURSE: Three weeks (full time)
Monitoring human immune responses
Immuno-monitoring is nowadays performed through a variety of molecular and cellular approaches, involving an array of cutting-edge technologies. This is key for human translational medicine studies on infectious diseases, epidemic outbreaks, vaccine trials, vaccination follow up, etc.
The goal of this practical course is to introduce students to investigate a research subject through a variety of state of the art experimental approaches, and to sensitize them to translational medicine studies.
Taking advantage of the New Institut Pasteur Center for Translational Medicine and the Milieu Intérieur Laboratory of Excellence Consortium, this practical course will focus on the characterization of age-dependent differences in the immune response of healthy individuals, in the presence or absence of chronic cytomegalovirus (CMV) infection. The response of each individual to an array of immune stimuli, targeting innate or adaptive immunity, will be investigated by immunophenotyping whole human blood samples and peripheral blood mononuclear cells (PBMC). A battery of approaches will be used, including multi-color flow cytometry, imaging flow cytometry, real-time PCR, RNA-sequencing, simple and multiplex ELISA. In addition, a subset of immune cells will be sorted and analyzed for the influence of immune stimulation on their metabolic activity. The students will have access to cutting-edge technologies available at the Technology Cores of the Center for Translational Medicine (CRT technology Cores) of the Institut Pasteur.
Sponsors: We thank the LabEx Milieu Intérieur for financial support.
LECTURES: two weeks (full time). Lectures will be dealing with advanced studies on major topics in cellular and molecular immunology, such as: development of the immune system, lymphocyte differentiation, molecular basis of antigen recognition by B and T lymphocytes, lymphocyte homeostasis, tolerance, cellular communication, immune responses, innate immunity and various aspects of immunopathology. Lectures are given by internationally renowned speakers.
BIBLIOGRAPHY ANALYSIS SEMINARS : three days of bibliography analysis, on six main immunology subjects will be, given by groups of students and supervised by two senior scientists per subject. These seminars aim at training students to the analysis and public presentation of a scientific work. These seminars are mandatory for students enrolled in Master programs and optional for other participants.
1) Attendance to ALL practical sessions, conferences and bibliography seminars is mandatory
2) Since Type-2 Biosafety laboratory facilities will be used during the course, updated tetanos and hepatitis B vaccination certificates are required for all the students. Positive HBV serology is required.
3) The course requires the students’ personal work during evenings and weekends. Students are asked not to take any engagements in that period of time. For instance, MDs or Pharmacists are asked not to get any hospital duties during the period of the course, including during the bibliography seminars week.
4) Candidates must have a good knowledge in Immunology (1st year master level, or equivalent), Biochemistry, Molecular Biology and Genetics.
5) Students should have good knowledge of English language to be able to understand full lectures, ask questions and deliver the bibliography analysis seminar in English. Understanding of French language is useful, but not mandatory, for daily interactions.
6) Students not enrolled in a M2 program of a partner Parisian university will finish the course on December 20, 2018. These students will have the exam on December 21, 2018.
7) Bibliography seminars will be run from December 19 to 20, 2018.
8) Exams will be January 11, 2019
The committee of the course will evaluate applications.
Lymphocyte cell biology unit
Center for Human Immunology - Institut Pasteur
C. Alcaide* (Université Paris-Diderot)
A. Alcover (Institut Pasteur),
A. Cumano (Institut Pasteur),
M. Hasan (Institut Pasteur),
M. Lucas-Hourani (Institut Pasteur),
S. Malot (Institut Pasteur),
V. Ponticelli (Institut Pasteur),
M. Sala (Institut Pasteur),
A. Six* (Université P. et M. Curie),
E. Tartour* (Université Paris-Descartes),
M. Viguier* (Université Paris-Diderot),
H. Waxin (Institut Pasteur),
A. Zider* (Université Paris-Diderot).
* University Representatives