Culture of strains

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Culture of freeze-dried bacterial strains

NB: The fundamental rules of manipulation in bacteriology must be applied
Disinfect the ampoule with alcohol and make a small file mark near the narrow part.

Once the ampoule is broken, resuspend its contents with 0.2 ml of nutritive broth (generally in a BHI agar medium) taken with a sterile Pasteur pipette.

Note about storage and culture conditions of bacterial strains (recto)
Note about storage and culture conditions of bacterial strains (verso)

For comments and questions, you should write to: crbip@pasteur.fr

Culture of cyanobacterial strains

As soon as a cyanobacterial strain has been received, a direct transfer is recommended, either in a gelose medium or in a liquid medium, according to the supplied strains and by respecting a lower volume than 50 ml. Cultures with large scales must be inoculated only with dense precultures and in a dilution around 1/20 ( v/v ).

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