Culture of freeze-dried bacterial strains
Disinfect the ampoule with alcohol and make a small file mark near the narrow part.
Once the ampoule is broken, resuspend its contents with 0.2 ml of nutritive broth (generally in a BHI agar medium) taken with a sterile Pasteur pipette.
Note about storage and culture conditions of bacterial strains (recto)
Note about storage and culture conditions of bacterial strains (verso)
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Culture of cyanobacterial strains
As soon as a cyanobacterial strain has been received, a direct transfer is recommended, either in a gelose medium or in a liquid medium, according to the supplied strains and by respecting a lower volume than 50 ml. Cultures with large scales must be inoculated only with dense precultures and in a dilution around 1/20 ( v/v ).