Culture of strains

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Culture of freeze-dried bacterial strains

NB: The fundamental rules of manipulation in bacteriology must be applied
Disinfect the ampoule with alcohol and make a small file mark near the narrow part.

Once the ampoule is broken, resuspend its contents with 0.2 ml of nutritive broth (generally in a BHI agar medium) taken with a Pasteur sterile pipette.

Note about storage and culture conditions of bacterial strains (recto)
Note about storage and culture conditions of bacterial strains (verso)

Culture of cyanobacterial strains

As soon as a cyanobacterial strain has been received, a direct transfer is recommended, either in a gelose medium or in a liquid medium, according to the supplied strains and by respecting a lower volume than 50 ml. Cultures with large scales must be inoculated only with dense precultures and in a dilution around 1/20 ( v/v ).

Any question ?

crbip@pasteur.fr

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