The Collection de l’Institut Pasteur (CIP), created in 1890 by Dr. Binot, is one of the first Biological Resources Centers (BRCs) established in the world. For more than a century now, the premises of the CIP are on the campus of the Institut Pasteur. Its missions are to ensure both the maintenance and the enrichment of its bacterial collection, on the one hand through collaborations with the scientists of the Institut Pasteur and on the other hand, by the deposition of strains of French and foreign researchers, and also the distribution of strains and associated information (properties, conservation, identification ...) as well as the development of a research activity.
|Strain collection at the time of Louis Pasteur||Strain collection today|
CIP is rich in about 20,000 bacterial strains belonging to 4,200 different species. The base of enrichment has been historically since the 1950s with:
- clinical strains (due to the proximity of Pasteur Hospital and Paris hospitals),
- strains from various National Reference Centers after the retirement of the responsible person: Streptococci, Staphylococci, Anaerobes, antibiotic-resistant strains, etc.
- recently described type strains of species,
- strains of the environment.
Characterization of resources
Characterization and identification of bacteria have long relied on phenotype. With the advent of the molecular biology, the bacterial identification was carried out using the gene that encodes the 16S rRNA and its routine sequencing was set up in 2006. Access to whole genomes is now possible for bacterial strains deposited since 2016 but also for those whose distribution batch is renewed, thanks to the collaboration of the CIP with the P2M platform of the Institut Pasteur.
Preservation of bacterial strains
The strains are preserved by lyophilization or freezing at -80 ° C or at -196 ° C in liquid nitrogen. As much as possible, two different methods of preservation and two types of storage for each strain are carried out in two different places.
The work that can be undertaken with the CIP is varied because of the diversity of resources maintained in the collection. The CIP has been able to constantly adapt to meet evolving user needs and current regulatory requirements. The importance of microbial resources for the present and the future is undeniable in view of their use in a wide variety of fields such as agronomy, food security, the environment, health, and biotechnology. Ensuring biosecurity and ensuring trade traceability are key elements of the future bio-economy as defined in 2007 in the OECD Best Practice Guidelines for Biological Resource Centers. To meet these challenges, the CIP has been successively certified ISO 9001, ISO 17025 and now NF S96-900, the specific standard for BRCs that has become an ISO standard (ISO 20387). It is part of the global network of micro-organism collections (WFCC), the World Data Center for Microorganisms (WDCM) and the ECCO (Organization of European Collections) office. Together with CRBIP, CIP has been involved in various European infrastructure projects, firstly EMBaRC (European Consortium of Microbial Resource Centers), a European program for the conservation and valorization of microbiological resources, then MIRRI (Infrastructure Pan-European Research Center for Microbial Resources) and EMBRIC (European Marine Biotechnology Research Infrastructure Group). At the national level, the CIP is one of the first CRBs to have been labeled IBiSA.
Lines of research
CIP is developing a research activity on identification, taxonomy (description of new species), bacterial strain preservation, antibiotic resistance and metabolomics. It is involved in collaborative projects with different funding at national and European level (ANR, One Health EJP ...).
Head of the CIP:
S. Coutellier - O. Mwana
M.G Goly - S. Hamon - E. Muhle - S. Favre-Rochex - P. Charles
For your comments and questions about the CIP, write to email@example.com
Strains are named according to the most recent valid names in the list of bacterial names with standing in nomenclatures (J.P. Euzeby) and on the DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen) site. http://www.dsmz.de
The names used (Skerman et al., 1980) have all been approved and published since 1980 in the International Journal of Systematic Bacteriology (Int. J. Syst. Bacteriol.) and since 2000 in the International Journal of Systematic and Evolutionary Microbiology (Int. J. Syst. Evol. Microbiol.)
If the strain has not yet been validated or published in the International Journal of Systematic Bacteriology, the Latin name assigned to the strain is placed in inverted commas.
Example : "Haemophilus agni" Kennedy et al.
Following informations is available:
The year :
It corresponds to the year of validation of the species.
This is the last denomination before the change of nomenclature.
For further information concerning changes of nomenclature, please consult the following sites: List of Bacterial Names with Standing in Nomenclatures (J.P. Euzeby) and DSMZ: bacterial nomenclature. http://ijs.sgmjournals.org
Group of risk:
This group of risk is based on the list published in the Official Journal of the European Communities, Directive 2000/54/CE of the European Parliament and the Council dated September 18, 2000.
The Recording number in the collection:
The letter "T" following the collection number of the strain indicates that the strain concerned is a standard strain.
Special conditions are applied for the distribution of strains marked ’ X’ and ’ XX’.