Protein biochemistry

Mechanisms of Living Organisms

This six-week theoretical and practical course focuses on the study of the relationships between the structure and function of proteins

Practical information

Institut Pasteur
January 6-February 7, 2020
September 8, 2019
In French


Credits for a Master 2 degree Sorbonne Université
Credits for a Master 2 degree Université de Paris
Institut Pasteur Diploma
University diploma Paris Diderot

The practical part is organized in three complementary modules:

1-The biosynthesis, translation and cellular refolding of proteins are studied through cloning, expression, genetic screening methods and methods for the production and purification of recombinant proteins.

2- The structural and functional characterization of isolated proteins is explored by physico-chemical and enzymatic approaches using spectroscopic, hydrodynamic and thermodynamic methods.

3- The analysis and interpretation of the structure-function relations are approached using computer tools of predictive computations and visualization of the molecular models by the control of the use of databases of sequences and structures.

The theoretical part includes about twenty conferences and discussions with Protéopôle actors of the Institut Pasteur and external specialists. The topics are directly related to the techniques used in the experimental part.

Candidates must have good knowledge of biochemistry, from the first year level of Master. Applications are evaluated by the Course Committee.

More informations


Jean-Michel Betton

Unité de Microbiologie structurale

CNRS, UMR 3528

Institut Pasteur


Nicolas Wolff

Unité Récepteurs-canaux

Institut Pasteur


Thierry Rose

Unité d'Analyse d'images biologiques

Institut Pasteur

Head(s) of laboratory practicals

Celia Caillet-Saguy

Unité Récepteurs-canaux

Institut Pasteur

5 weeks
Members of the Course committee

J.-M. Betton (Institut Pasteur),

T. Foulon* (Sorbonne Université),

B. Friguet (Sorbonne Université),

Y. Gaudin (CNRS),

O. Lequin (Sorbonne Université- LBM)

M. Lucas-Hourani (Institut Pasteur),

S. Malot (Institut Pasteur),

Br. Miroux (Institut de Biochimie et Physico-Chimie)

V. Ponticelli (Institut Pasteur),

F. Rodrigues-Lima* (Université de Paris),

T. Rose (Institut Pasteur),

M. Sala (Institut Pasteur),

J. Vinh (ESPCI),

H. Waxin (Institut Pasteur),

N. Wolff (Institut Pasteur)

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