This one-week course is designed to evaluate diagnostic tests, from validation of tests in the laboratory to their use in the field.
The objective of this course is to provide science, medicine and public health students with training in diagnostic test evaluation. The knowledge must be supplemented by statistical bases (CESAM type or introduction to biostatistics) and notions in the biomedical field.
The themes that are developed concern:
- The different stages of validation of a diagnostic test: from the laboratory validation to the use in the field.
- Protocol development with identification and discussion of biases.
- The main measures for evaluating the accuracy and reproducibility of the tests (sensitivity, specificity, ROC curves, kappa, Bland-Altman methods, etc.).
- Models used for imperfect or non-existent “gold standard” tests.
- The quality criteria of a diagnostic study.
- Cost-effectiveness comparisons.
- All these theoretical notions will be accompanied by practical examples using software.
- Introduction to the evaluation of diagnostic tests.
- The different protocols: phase 1 (proof of concept), phase 2 (case-control), phase 3 (prospective study).
- The different biases.
- The quality of diagnostic studies; the STARD and QUADAS tools.
- The main evaluation measures: qualitative tests (sensitivity, specificity, VPP, VPN, Kappa coefficient) and quantitative tests (ROC curves, Bland and Altman diagram, intra-class correlation coefficient, coefficient of variation).
- The question of "gold standard" and its modeling (latent class analysis).
- Evaluation of the cost-effectiveness of different diagnostic algorithms.
Applications are evaluated by the Course Committee.
Institut de médecine tropicale
Unit of epidemiology of infectious diseases
Institut Pasteur of Dakar
Institut de Médecine Tropicale
Lenaig Le Fouler
Unit of Pharmacoepidemiology & Infectious Diseases
M. Boelaert (Institut Médecine Tropicale, Anvers),
E. Coeffier-Vicart (Institut Pasteur),
L. Le Fouler (Institut Pasteur)
S. Malot (Institut Pasteur),
J. Menten (Institut de Médecine Tropicale, Anvers, Belgique)
V. Ponticelli (Institut Pasteur),
M. Sala (Institut Pasteur),
M. Vray (Institut Pasteur),
H. Waxin (Institut Pasteur).