DEFINITION Vibrio metschnikovii initiation
factor 3 (infC) gene
(partial cds), ribosomal protein L35 (rpmI) gene,
ribosomal
protein L20 (rplT) and super-integron InVme
site-specific recombinase VmeIntIA
(VmeintIA)
genes, complete cds; and ORFs, first gene cassettes.
ACCESSION ;
KEYWORDS .
SOURCE Unknown.
ORGANISM Unknown.
Unclassified.
REFERENCE 1 (bases 1 to 3349)
AUTHORS Rowe-Magnus,D.A., Guerout,A.
and Mazel,D.
TITLE Direct Submission
JOURNAL Submitted (24-NOV-2000)
Biotechnologies, Institut Pasteur, 25 rue
du
Dr Roux, Paris 75724, France
FEATURES Location/Qualifiers
source 1..3349
gene <1..332
/gene="infC"
CDS <1..332
/gene="infC"
/note="initiation
factor 3 [intronless gene]"
/codon_start=3
/transl_table=11
/product="IF3"
/translation="GKFLFEKSKAAEEQKKKQKQVQIKEIKFRPGTDIGDYQVKLRNL
TSFLEEGNKVKVTIRFRGREMAHQEIGVDVLNRLKEDTADIAVVESFPSRIEARQMIM
MLAPKKK"
gene 432..626
/gene="rpmI"
CDS 432..626
/gene="rpmI"
/note="ribosomal
protein (large subunit); [intronless
gene]"
/codon_start=1
/transl_table=11
/product="L35"
/translation="MPKMKTNKGAAKRFKKTAGGIKYKHATKRHILTKRTTKNKRQLR
PNSILPKCEVAGVMRMLPYA"
gene 668..1021
/gene="rplT"
CDS 668..1021
/gene="rplT"
/note="ribosomal
protein (large subunit); [intronless
gene]"
/codon_start=1
/transl_table=11
/product="L20"
/translation="MPRVKRGVQARARHKKILKQAKGYYGARSRVYRVAFQAVTKAGQ
YAYRDRRTKKRQFRQLWIARINAASRQNGLSYSRFINGLKKASIEIDRKILADIAVFD
KAAFSVLVEKAKAAL"
gene complement(1069..2031)
/gene="VmeintIA"
CDS complement(1069..2031)
/gene="VmeintIA"
/note="site-specific
recombinase (Tyrosine recombinase);
V.
metschnikovii super-integron integrase"
/codon_start=1
/transl_table=11
/product="VmeIntIA"
/translation="MKSQFLLSVKEYMQVRYYANKTIESYIHWITQYVVFHNKTHPNL
LGSRDVEQFLTYLAVEKKVATKTQSLAFEALSFLYREIIKEPLTLEMKFQKSQLERKL
PIVMTREEIKRFFNHVDPKYQLHIKLLYGSGLRLMECVRLRVQDIDFDYGALRIWQGK
GGKNRTVTLAKELHPLIKEQISLAKRYYDKDMEAKGYGGVWLPNALNSKYPTAPFEFH
WHYLFPSNALSVDQESNLLRRHHINETSLQRTVKRAVKEAEIKKSVSCHTLRHSFATH
LLESGADIRTVQEQLGHADVSTTQIYTHVLDRGANGVLSPLSRL"
misc_recomb 2247..2248
/note="attI,
cassette integration site"
gene 2299..3177
/gene="ORF1"
CDS 2299..3177
/gene="ORF1"
/note="
[intronless gene]"
/codon_start=1
/transl_table=11
/product="ORFVMe1"
/translation="MECRSCGKSDFKLTIQKNHNGGTTRYRRCVTCNSNIGAALSLTT
FEQLKSSGYSVVDLGAGSQQSLVSNNSLPKSTLFKNEKEVQDWFFNVFDEWFYIAKEV
PGKHLVEDMKVRMDFMLYPRSSLSDMGFYKGWFGVEVKLFPNHREELSAPSKALWQAV
SYNDSEFYLNQSKTANFNVSSIEGATKPFYTMIFSNRSFNKGKNEYLNQNGEKQRNQF
DYIMRTISTVGLYAKVGMVNIIESSDNSNIKKWSFDFGRETYCSFVRETNDTYRQTQF
KLHNEEIIKKIRVGHQ"
repeat_unit 3185..3304
/note="VMeR,
attC site (also called 59 base element)
family"
misc_recomb 3301..3302
/note="attC
site recombination point"
BASE COUNT 1032 a 594
c 685 g 1038 t
ORIGIN
1 atggcaagtt
cctctttgaa aagagtaaag ctgctgaaga gcagaagaag aagcaaaaac
61 aggtccagat
taaggaaata aaattccgtc ctggaactga tatcggagac tatcaggtaa
121 aactacgcaa cctgactagc
ttccttgaag aaggcaacaa agtgaaagta actattcgct
181 tccgtggccg tgagatggcg
caccaagaaa tcggtgttga tgttttgaat cgtttgaaag
241 aagatacggc tgatatcgcg
gtagtagaat ctttcccgag cagaattgaa gcacgccaga
301 tgatcatgat gcttgcccct
aaaaagaagt aattaacggc attgcaagta ataaaaccta
361 ggattaaaag ctgggtttta
ttcgccctaa ttacattgtt attaaactac tacaatgcgg
421 agttattcat catgcctaag
atgaaaacca acaaaggtgc tgctaagcgt tttaagaaaa
481 cagctggtgg tattaagtac
aagcacgcta ctaaacgtca catcctgact aagcgtacta
541 ctaagaacaa gcgtcaacta
cgccctaact caatccttcc gaaatgtgaa gtggctggtg
601 ttatgcgcat gttgccatac
gcttaattct ttttagttta tttatcgttt agtttaggag
661 agacataatg cctcgcgtaa
aacgtggtgt acaagctcgt gcacgtcata agaagattct
721 aaaacaagct aaaggttact
atggtgcacg ttcacgtgtt taccgcgtag ctttccaagc
781 cgttactaaa gcaggccaat
atgcttaccg tgaccgtcgc actaaaaagc gtcagttccg
841 tcagctatgg atcgcgcgta
ttaacgctgc atctcgtcaa aatggtctat cttacagccg
901 tttcatcaat ggtcttaaga
aagcatctat cgagatcgat cgtaagatcc tcgcagatat
961 cgcagtattc gacaaagcag
cattttctgt tcttgttgag aaagcgaaag ctgctctgta
1021 atagagtttt aactgtttaa
tcaaaaggag ggcttaggct ctcctttttt ataagcgaga
1081 taaagggctc aataccccgt
ttgctcctct atccaataca tgagtataaa tttgtgtcgt
1141 tgagacgtca gcatgaccaa
gctgctcttg taccgttcga atatcagctc ctgattcgag
1201 aagatgggtc gcaaatgagt
gtcttagcgt atggcaagat actgattttt taatttcagc
1261 ttcttttact gctcttttta
cggttcgttg taatgaggtt tcattaatat gatgccgacg
1321 taataagttg ctttcctgat
caaccgataa tgcgttagat ggaaataagt aatgccaatg
1381 aaattcgaat ggtgctgtcg
gatatttgct atttaatgcg ttaggcaacc atacgccacc
1441 ataacctttc gcttccatat
ctttatcgta gtatcgtttt gcgagtgata tttgttcctt
1501 gattaaaggg tgtaactctt
tggccagtgt tactgttcga tttttccctc cttttccctg
1561 ccatatcctc aaagctccat
aatcaaaatc gatatcttgt acacgtaagc gaacgcattc
1621 catcaatctt agtcctgaac
cataaagtag tttgatgtga agctgatatt ttggatcaac
1681 atgattaaag aagcgtttta
tttcttctcg cgtcatgacg atcggtagct ttctttccaa
1741 ttgagatttt tggaatttca
tttccagtgt caatggctct ttgatgatct ccctatacaa
1801 aaaactcagt gcttcaaaag
ccaatgattg tgttttggtt gctacttttt tttcaacagc
1861 taagtaggtt aaaaactgtt
cgacatcacg agagcctaat agattagggt gagttttatt
1921 atgaaagacc acatattgcg
ttatccaatg tatataagat tctatagttt tgttagcata
1981 atacctgact tgcatatatt
cctttacgct aagcagaaat tgagatttca tacataaatc
2041 ctttgctgtt tttatataca
gtgatgtggg ttttataggc tgtcaataac taagtgacat
2101 tttgccgttt atttagatct
gcttctcatt gcgatggtgt ataacatgat gaatcagatg
2161 gttattatgt tacgattgtt
cgacctttgc tctaaaaagg cggcggtagc gtggaagaaa
2221 agacggcatg tttcagtgta
ataagcgtta ggtgaaagtt ttggcaatag aattgtgatt
2281 gatgatttaa gggtaaaaat
ggaatgtaga agctgtggaa aaagtgactt caagctcact
2341 attcaaaaaa atcataatgg
cgggactact aggtatcgta gatgtgtaac ttgcaactcg
2401 aatattggcg cagcattgag
cctaactact ttcgaacaac taaaaagtag tggttattca
2461 gtcgttgatt taggggctgg
ttcacaacaa agcttagtca gcaacaatag tttgccaaaa
2521 tcaaccttgt tcaagaatga
aaaagaagtc caagattggt tttttaatgt ttttgatgaa
2581 tggttctata ttgcaaaaga
agttccaggt aagcaccttg ttgaggacat gaaagtccgt
2641 atggacttta tgctttaccc
aagatcatca ttatctgata tgggctttta taaaggctgg
2701 tttggagtag aagttaaact
atttccaaat catcgagaag aattatctgc tcccagtaaa
2761 gctttatggc aagcagttag
ttataacgat agtgagttct atttaaatca atcaaaaaca
2821 gcaaacttta atgtttcaag
tattgaaggt gctacaaaac cattctacac aatgattttt
2881 agtaatcgca gttttaataa
aggtaaaaat gagtacctta accaaaatgg agaaaaacag
2941 agaaatcaat tcgattatat
aatgcgtaca attagtactg tcgggctata tgcaaaagtt
3001 ggaatggtta atattatcga
atctagtgat aactctaaca tcaaaaagtg gagctttgat
3061 tttggtagag aaacatattg
ctcctttgta cgagaaacta atgatactta cagacaaacc
3121 caatttaaat tgcataatga
agaaataatt aaaaaaataa gagttgggca ccagtaattt
3181 cacctaacaa ggcgttaaag
agggattcac aacgcgcggc attttcattg tgcgttgatt
3241 ttattggttt tagtgctatg
cggtgagtta gtagttgcgt tgctcacccc ttaacgcggc
3301 gttaaatgct ttcagatgta
cgcgaaaatc tgagtatgtg acctttaaa
// |
