LOCUS       AF324945     1283 bp    DNA             BCT       07-FEB-2001
DEFINITION  Vibrio cholerae super-integron periplasmic sulfate binding protein
            SBP (sbp) gene, complete cds.
ACCESSION   AF324945
KEYWORDS    .
SOURCE      Vibrio cholerae.
  ORGANISM  Vibrio cholerae
            Bacteria; Proteobacteria; gamma subdivision; Vibrionaceae; Vibrio.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Rowe-Magnus,D.A., Guerout,A.-M., Ploncard,P., Dychinco,B.,
            Davies,J. and Mazel,D.
  TITLE     The evolutionary history of chromosomal super-integrons
provides an
            ancestry for multi-resistant integrons
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 98 (2), 652-657 (2001)
REFERENCE   2  (bases 1 to 1283)
  AUTHORS   Rowe-Magnus,D.A. and Mazel,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-NOV-2000) Biotechnologies, Institut Pasteur, 25 rue
            du Dr Roux, Paris 75724, France
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Vibrio cholerae"
                     /strain="CIP 62.14"
                     /db_xref="taxon:666"
                     /note="super-integron cassette"
     gene            complement(67..1053)
                     /gene="sbp"
     CDS             complement(67..1053)
                     /gene="sbp"
                     /note="complements Escherichia coli mutant"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic sulfate binding protein SBP"
 
/translation="MQKLLLVLTVLTFGSTALANDSTLLNVSYDPTRELYQEYNQAFT
TYWASQKKQHIKINQSHGGSGKQAITVINGIESDVVTLALAQDIDAIASSGKIAKDWQ
SRLPHNSAPYTSTIVFLVRKGNPKNIQDWDDLTRENIEVITPNPKTSGGARWNYLAAL
GYALTKSNNDEVYAQQFVKSIYSNVKVLDTGARGSSNTFIEREIGDVLITWENEALLA
INKIENHNVEIIIPSTSILAEPTVSIVDKVVDKKGTRAMAEAYLEYLYSPVGQEIAAK
NFYRPRDKNIAIKYSKQFPELNLFTIDKFSGWDEAHKKHFSQGGIFDQLSKR"
     misc_recomb     1165..1283
                     /note="VCR; attC site; 59 base element"
BASE COUNT      337 a    242 c    250 g    454 t
ORIGIN
        1 ttaggattta ctcaagttga cagaagcaga aagctataga tttcagattt ctgcctcatt
       61 tttaaactac cttttactta actgatcgaa tattccgcct tgcgaaaaat gtttcttatg
      121 ggcttcatcc catccagaaa acttatctat agtaaataag tttagttctg gaaactgctt
      181 ggaatattta atggctatgt ttttatcccg aggtctataa aagtttttgg ctgcaatttc
      241 ctgtcctaca ggagaataaa gatactcaag atatgcttca gccatagcgc gagttccttt
      301 tttatcaacg actttatcta ctatcgatac tgttggttcc gctaatatcg acgtgctcgg
      361 aattattatt tctacattgt gattttcgat cttattaata gccaataacg cttcattttc
      421 ccatgtaatc agaacatcac caatttctcg ttcaataaag gtatttgatg aacctctagc
      481 accagtatct aggactttaa cattgctata gattgatttt acaaattgtt gggcatagac
      541 ttcgtcgtta ttactttttg tcagtgcgta gcccaaagct gctaggtaat tccaacgagc
      601 tcctccagaa gttttcggat ttggtgtgat gacttcaata ttctctctgg ttaaatcatc
      661 ccaatcttga atgttttttg ggttgccctt acggacaaga aaaacaatag tggatgtgta
      721 tggagccgaa ttatgtggta gccgagattg ccagtccttt gctatttttc ctgagctagc
      781 gattgcatca atatcttgag ccaatgccaa agttaccaca tcagattcaa taccattaat
      841 aactgtaatt gcctgtttac cagagcctcc atgagattga tttatcttga tgtgttgttt
      901 tttttggctt gcccaatatg tagtaaatgc ctgattatac tcttggtata gctcacgagt
      961 aggatcgtat gagacattga gtaaagtgga atcgtttgct agggctgtgc ttccgaaagt
     1021 gagcactgtt aaaactaaaa gtaatttctg catcttaata tccttatgtg cgttgggttc
     1081 acataaatta gcatggtgct tatgtattaa ttacttattt tatctgattt catattcact
     1141 ttggttatat tgatgccgta aacctaacaa acgcctcaag agggactgtc aacgcgtggc
     1201 gtttccagtc ccattgagcc gtggtggttt cggttgttgt gtttgagttt agtgttatgc
     1261 gttgtcagcc ccttaggcgg gcg
//