Time-lapse movie processing for DISC analysis.
Following intravital imaging of GFP+ cells in the presence of fluorescent Fab fragments (e.g. in the spleen), time-lapse movies are subjected to 3D cell tracking and cell segmentation. Time-lapse movies can then be further processed by applying a mask corresponding to the segmented cell volume. GFP and Fab fluorescence are then automatically extracted for each cell at each time point.