Related articles:

• Moreau H, Lemaître F, Terriac E, Azar G, Piel M, Lennon-Dumenil A, Bousso P. (2012). Dynamic In Situ Cytometry uncovers T cell receptor signaling during immunological synapses and kinapses in vivo. Immunity, 37(2);351-363.

• Beuneu H, Deguine J, Bouvier I, Di Santo JP, Albert ML, Bousso P. (2011). Cutting Edge: A dual role for type I IFNs during polyinosinic-polycytidylic acid-induced NK cell activation: J Immunol. 187 (5):2084-2088.

• Beuneu H, Lemaître F, Deguine J, Moreau H, Bouvier I, Garcia Z, Albert ML, Bousso P. (2010). Visualizing the functional diversification of CD8+ T cell responses in lymph nodes. Immunity, 33(3);412-423.

• Azar GA, Lemaître F, Robey EA, Bousso P (2010). Subcellular dynamics of T cell immunological synapses and kinapses in lymph nodes. Proc. Natl. Acad. Sci. USA. 107(8):3675-3680.

• Beuneu H, Deguine J, Breart B, Mandelboim O, Di Santo JP, Bousso P (2009). Dynamic behavior of NK cells during activation in lymph nodes. Blood 114(15):3227-3234.

• Celli S, Lemaitre F, Bousso P (2007). Real-time manipulation of T cell-dendritic cell interactions in vivo reveals the importance of prolonged contacts for CD4+ T cell activation. Immunity 27(4):625-634.

• Garcia Z, Pradelli E, Celli S, Beuneu H, Simon A, Bousso P (2007). Competition for antigen determines the stability of T cell-dendritic cell interactions during clonal expansion. Proc. Natl. Acad. Sci. USA. 104(11):4553-4558.

• Beuneu H, Garcia Z, Bousso P (2006). Cutting edge: Cognate CD4 help promotes recruitment of antigen-specific CD8 T cells around dendritic cells. J. Immunol. 177(3):1406-1410.

• Celli S, Garcia Z, Bousso P (2005). CD4 T cells integrate signals delivered during successive DC encounters in vivo. J. Exp. Med. 202(9):1271-1278.

• Bousso P, Robey EA (2003). Dynamics of CD8+ T cell priming by dendritic cells in intact lymph nodes. Nat. Immunol. 4(6):579-585.

Dynamics of Immune Responses Lab

Adaptive immune responses are initiated in secondary lymphoid organs through cellular interactions between antigen-specific T cells and antigen-presenting dendritic cells. One of the aim of our lab is to better understand how T cells collect activation signals in vivo. Using intravital multiphoton imaging, we are therefore studying how T cell-DC contacts are regulated and how activation signals are delivered during individual interactions. These studies help identify fundamental mechanisms shaping the efficacy of T cell activation and differentiation. Recently, we have extended these questions to natural killer cells.

T cells forming stables interactions with dendritic cells in the lymph node upon antigen recognition.
T cells (red) and dendritic cells (green) are imaged in the popliteal lymph node of a live mouse. Antigen is delivered to DCs at t=0. Click here to see the movie.

Dynamics of the immunological synapse during T cell-B cell interactions in the lymph node.
T cells expressed a LAT-GFP fusion protein. B cells are shown in red. Click here to see the movie.

T cell functional heterogeneity is initiated prior to the first cell division.
T cells isolated from an IFN-g YFP reporter mice and labeled with ta red are imaged during their first division after activation. Yellow and green T cells have activated the IFN-g gene at the time of division, whereas red T cells have not. Click here to see the movie.

In vivo imaging TCR signaling in real-time using CD62L shedding.
GFP-expressing T cells (green) are labeled in vivo with fluorescent anti-CD62L Fab (red membrane staining). Antigen is delivered at t=0 resulting in T cell stop and progressive decrease of CD62L staining. Intravital imaging was performed in the spleen. Click here to see the movie.

NK cell motility in lymph nodes.
NK cells (red), T cells (blue) and DCs (green) are imaged at steady state in the lymph node. Click here to see the movie.