> Pathogénie microbienne moléculaire - INSERM U389
• Summary
• Saga Shigella
• Genetics
• Cell Biology
• Inflammation
• Immunology
• Vaccines
A GLOBAL ANALYSIS OF THE MOLECULAR PATHOGENESIS OF SHIGELLA INFECTION: FROM CELLULAR MICROBIOLOGY TO TISSUE MICROBIOLOGY (1990-NOW).
The creation of “Unité de Pathogénie Microbienne Moléculaire” in 1989, with Nancy Guillen, Armelle Phalipon, Claude Parsot and Guy Tran Van Nhieu, was an opportunity to focus research objectives on Shigella and Entamoeba histolytica, an enteroinvasive protozoan parasite as a model microorganisms to study the rupture, invasion and inflammatory destruction of an epithelial barrier byl pathogen. The work on E.histolytica will not be mentioned here since Nancy Guillen has recently taken the lead of her own new “Unité de Biologie Cellulaire du Parasitisme”(Please refer to this web site for informations).
The major research aims of PMM have been (i) to identify the repertoire of Shigella genes encoding the invasive phenotype, their regulation and the functional organisation of the corresponding proteins in an “invasive apparatus”; (ii) to decipher the molecular cross talks occurring between the bacterium and the mammalian cell targets that lead to its internalisation, escape into the cytoplasm, intracellular motility and cell-to-cell spread; (iii) to establish in vitro and in vivo models to study how bacteria invade, disrupt and cause the inflammatory destruction of the intestinal epithelium; and (iv) to identify the inductive and effective mechanisms of the innate and adaptive immune responses against Shigella.
Genetic, molecular and cellular bases of Shigella invasion of epithelial cells and cell-to-cell spread. Shigella has become one of the paradigms to study entry of a bacterial pathogen into a non-phagocytic cell.