Bacterial Artificial Chromosome Libraries of
Mycobacterium tuberculosis H37Rv and M. bovis BCG Pasteur
Ordered M. tuberculosis H37Rv and M. bovis BCG Pasteur 1173
Bacterial Artificial Chromosome (BAC) maps, originally overlaid on the M. tuberculosis H37Rv DraI and AsnI physical/integrated map, (established by Philipp et al., 1996). 'Rv' clones are M. tuberculosis pBeloBAC11 clones, 'X' clones indicate M. bovis BCG pBeloBAC11 clones, 'XE' clones are M. bovis BCG pBACe3.6 clones. The location of each BAC clone relative to the genomic position on the M. tuberculosis genome (in kilobases) is marked by small numbers.
The library contains about 5,000 BAC clones, with inserts ranging in size from 25 to 104 kb, representing theoretically a 70-fold coverage of
the M. tuberculosis genome (4.4 Mb). A seleted set of clones covering almost all of the genome of M. tuberculosis is shown in the BAC-MAP above.
This set of clones was also used for the development of BAC arrays, in an attempt to identify polymorphic regions in the genomes of tubercle bacilli.
These clones are listed with their coordinates on the H37Rv genome below.
They are also shown, superimposed on a physical DraI map in the figure below.
In addition, a second BAC library with inserts from the vaccine strain
of Mycobacterium bovis BCG Pasteur was constructed and used for
comparative genomics of members of the M. tuberculosis
complex.(Mol. Microbiol. 32: 643-656).
BAC-Clone numbers and their position on the M. tuberculosis genome
(MTBH37RV)
