FT CDS 1..1566 FT /class="II.A.5" FT /colour=2 FT /gene="dnaA" FT /gene="ML0001" FT /note="Similar to M. tuberculosis dnaA, chromosomal FT replication initiator protein, SW:DNAA_MYCTU (P49993) (507 FT aa); Fasta score E(): 0, 87.2% identity in 507 aa overlap FT and Mycobacterium smegmatis dnaA, SW:DNAA_MYCSM (P49992) FT (495 aa); Fasta score E(): 0, 69.2% identity in 441 aa FT overlap. Previously sequenced as SW:DNAA_MYCLE (P46388) FT (521 aa); Fasta score E(): 0, 99.8% identity in 521 aa FT overlap. Contains Pfam match to entry PF00308 bac_dnaA, FT Bacterial dnaA protein. Contains PS00017 ATP/GTP-binding FT site motif A (P-loop). Contains PS01008 DnaA protein FT signature." FT /product="putative chromosomal replication initiator FT protein" FT /tb_orthologue="dnaA" FT misc_feature 544..1482 FT /colour=0 FT /note="Pfam match to entry PF00308 bac_dnaA, Bacterial FT dnaA protein, score 712.20, E-value 8.3e-240" FT misc_feature 664..687 FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT misc_feature 1426..1482 FT /colour=8 FT /note="PS01008 DnaA protein signature" FT CDS 2081..3280 FT /EC_number="2.7.7.7" FT /class="II.A.5" FT /colour=2 FT /gene="dnaN" FT /gene="ML0002" FT /note="Similar to M. tuberculosis dnaN, DNA polymerase FT III, [beta] subunit, SW:DP3B_MYCTU (Q50790) (402 aa); FT Fasta score E(): 0, 80.9% identity in 403 aa overlap and FT Mycobacterium smegmatis dnaN, SW:DP3B_MYCSM (P52851) (397 FT aa); Fasta score E(): 0, 77.3% identity in 397 aa overlap. FT Previously sequenced as SW:DP3B_MYCLE (P46387) (399 aa); FT Fasta score E(): 0, 100.0% identity in 399 aa overlap. FT Contains Pfam match to entry PF00712 DNA_pol3_beta, DNA FT polymerase III beta subunit." FT /product="putative DNA polymerase III, [beta] subunit" FT /tb_orthologue="dnaN" FT misc_feature 2117..3214 FT /colour=0 FT /note="Pfam match to entry PF00712 DNA_pol3_beta, DNA FT polymerase III beta subunit, score 592.70, E-value FT 2.2e-174" FT CDS 3281..4438 FT /class="II.A.5" FT /colour=2 FT /gene="recF" FT /gene="ML0003" FT /note="Similar to M. tuberculosis recF, DNA replication FT and SOS induction protein, SW:RECF_MYCTU (Q59586) (385 FT aa); Fasta score E(): 0, 76.4% identity in 385 aa overlap FT and Mycobacterium smegmatis recF, SW:RECF_MYCSM (P50916) FT (384 aa); Fasta score E(): 0, 70.0% identity in 383 aa FT overlap. Previously sequenced as SW:RECF_MYCLE (P46391) FT (385 aa); Fasta score E(): 0, 99.5% identity in 385 aa FT overlap. Contains 2 Pfam matches to entry PF00470 RecF, FT RecF protein. Contains PS00617 RecF protein signature 1. FT Contains PS00017 ATP/GTP-binding site motif A (P-loop). FT Contains PS00618 RecF protein signature 2." FT /product="putative DNA replication and SOS induction FT protein" FT /tb_orthologue="recF" FT misc_feature 3287..3415 FT /colour=0 FT /note="Pfam match to entry PF00470 RecF, RecF protein, FT score 18.90, E-value 0.00024" FT misc_feature 3368..3391 FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT misc_feature 3614..3691 FT /colour=8 FT /note="PS00617 RecF protein signature 1" FT misc_feature 4100..4423 FT /colour=0 FT /note="Pfam match to entry PF00470 RecF, RecF protein, FT score 5.20, E-value 1.5" FT misc_feature 4244..4297 FT /colour=8 FT /note="PS00618 RecF protein signature 2" FT CDS 4435..5004 FT /class="V" FT /colour=10 FT /gene="ML0004" FT /note="Similar to hypothetical proteins from mycobacteria FT e.g. M. tuberculosis Rv0004, ypothetical protein, FT TR:P71573 (EMBL:AL123456) (187 aa); Fasta score E(): 0, FT 77.2% identity in 167 aa overlap. Previously sequenced as FT TR:Q50181 (EMBL:Z70722) (199 aa); Fasta score E(): 0, FT 99.5% identity in 187 aa overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv0004" FT RBS 5211..5216 FT /note="possible RBS" FT CDS 5229..7265 FT /EC_number="5.99.1.3" FT /class="II.A.5" FT /colour=2 FT /gene="gyrB" FT /gene="ML0005" FT /note="Similar to M. tuberculosis gyrB, DNA gyrase subunit FT B, SW:GYRB_MYCTU (P41514) (686 aa); Fasta score E(): 0, FT 87.5% identity in 679 aa overlap and to Mycobacterium FT smegmatis gyrB, SW:GYRB_MYCSM (P48355) (675 aa); Fasta FT score E(): 0, 85.0% identity in 679 aa overlap. Previously FT sequenced as SW:GYRB_MYCLE (Q59533) (697 aa); Fasta score FT E(): 0, 100.0% identity in 678 aa overlap. Contains Pfam FT match to entry PF00986 DNA_gyraseB_C, DNA gyrase B FT subunit, carboxyl terminus. Contains Pfam match to entry FT PF00204 DNA_topoisoII, DNA topoisomerase II (N-terminal FT region). Contains PS00177 DNA topoisomerase II signature." FT /product="putative DNA gyrase subunit B" FT /tb_orthologue="gyrB" FT misc_feature 5274..7001 FT /colour=0 FT /note="Pfam match to entry PF00204 DNA_topoisoII, DNA FT topoisomerase II (N-terminal region), score 1031.00, FT E-value 0" FT misc_feature 6606..6632 FT /colour=8 FT /note="PS00177 DNA topoisomerase II signature" FT misc_feature 7029..7229 FT /colour=0 FT /note="Pfam match to entry PF00986 DNA_gyraseB_C, DNA FT gyrase B subunit, carboxyl terminus, score 154.30, E-value FT 2.1e-42" FT CDS 7318..11067 FT /EC_number="5.99.1.3" FT /class="II.A.5" FT /colour=2 FT /gene="gyrA" FT /gene="ML0006" FT /note="Similar to M. tuberculosis gyrA, DNA gyrase subunit FT A, SW:GYRA_MYCTU (Q07702) (838 aa); Fasta score E(): 0, FT 91.1% identity in 695 aa overlap and to Mycobacterium FT smegmatis gyrA, SW:GYRA_MYCSM (P48354) (842 aa); Fasta FT score E(): 0, 88.2% identity in 692 aa overlap. Previously FT sequenced as SW:GYRA_MYCLE (Q57532) (1273 aa); Fasta score FT E(): 0, 99.9% identity in 1246 aa overlap. Contains a FT probable intein from aa 131 to 550 inclusive. Contains 2 FT Pfam matches to entry PF00521 DNA_topoisoIV, DNA FT gyrase/topoisomerase IV, subunit A. Contains PS00018 FT EF-hand calcium-binding domain. Contains PS00881 Protein FT splicing signature." FT /product="putative DNA gyrase subunit A" FT /tb_orthologue="gyrA" FT misc_feature 7435..7707 FT /colour=0 FT /note="Pfam match to entry PF00521 DNA_topoisoIV, DNA FT gyrase/topoisomerase IV, subunit A, score 239.20, E-value FT 6e-75" FT misc_feature 8947..8970 FT /colour=8 FT /note="PS00881 Protein splicing signature" FT misc_feature 8968..10053 FT /colour=0 FT /note="Pfam match to entry PF00521 DNA_topoisoIV, DNA FT gyrase/topoisomerase IV, subunit A, score 669.60, E-value FT 7.4e-211" FT misc_feature 10090..10128 FT /colour=8 FT /note="PS00018 EF-hand calcium-binding domain" FT repeat_region complement(11147..11477) FT /note="shares 93% sequence identity with bases FT 3145210..3145541" FT RBS 11184..11189 FT /note="possible RBS" FT CDS 11195..12106 FT /class="II.C.4" FT /colour=3 FT /gene="ML0007" FT /note="Similar to M. tuberculosis Rv0007, hypothetical FT protein, TR:P71575 (EMBL:AL123456) (304 aa); Fasta score FT E(): 0, 53.8% identity in 316 aa overlap. Previously FT sequenced as TR:O32870 (EMBL:Z70722) (303 aa); Fasta score FT E(): 0, 99.7% identity in 303 aa overlap. Contains FT hydrophobic, possible membrane-spanning regions." FT /note="Contains a region identical to ML2630" FT /product="putative membrane protein" FT /tb_orthologue="Rv0007" FT tRNA 12194..12270 FT /colour=4 FT /note="tRNA Ile anticodon GAT, Cove score 88.84" FT tRNA 12404..12479 FT /colour=4 FT /note="tRNA Ala anticodon TGC, Cove score 86.68" FT CDS complement(12856..13230) FT /class="VI" FT /colour=8 FT /gene="ML0008" FT /ml_unique FT /note="Unknown function." FT /product="hypothetical protein" FT RBS 13763..13766 FT /note="possible RBS" FT CDS 13775..13966 FT /class="VI" FT /colour=8 FT /gene="ML0009" FT /ml_unique FT /note="Unknown function." FT /product="hypothetical protein" FT CDS complement(15426..15767) FT /class="V" FT /colour=0 FT /gene="ML0010" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv0008c (Best blastx score 124)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS 15993..16541 FT /EC_number="5.2.1.8" FT /class="II.A.6" FT /colour=2 FT /gene="ppiA" FT /gene="ML0011" FT /note="Similar to M. tuberculosis ppiA, peptidyl-prolyl FT cis-trans isomerase, SW:CYPA_MYCTU (P71578) (182 aa); FT Fasta score E(): 0, 90.1% identity in 182 aa overlap and FT Streptomyces chrysomallus ScCypB, peptidyl-prolyl FT cis-trans isomerase B, TR:P77949 (EMBL:U64692) (175 aa); FT Fasta score E(): 0, 63.3% identity in 166 aa overlap. FT Contains Pfam match to entry PF00160 pro_isomerase, FT Cyclophilin type peptidyl-prolyl cis-trans isomerase." FT /note="Similar to ML0492" FT /product="putative peptidyl-prolyl cis-trans isomerase" FT /tb_orthologue="ppiA" FT misc_feature 16062..16529 FT /colour=0 FT /note="Pfam match to entry PF00160 pro_isomerase, FT Cyclophilin type peptidyl-prolyl cis-trans isomerase, FT score 193.30, E-value 2.8e-55" FT CDS complement(16566..16979) FT /class="II.C.4" FT /colour=3 FT /gene="ML0012" FT /note="Similar to M. tuberculosis Rv0010c, hypothetical FT protein, SW:Y010_MYCTU (P71580) (141 aa); Fasta score E(): FT 0, 71.5% identity in 137 aa overlap. Contains hydrophobic, FT possible membrane-spanning regions." FT /product="putative membrane protein" FT /tb_orthologue="Rv0010c" FT CDS complement(17134..17415) FT /class="V" FT /colour=10 FT /gene="ML0013" FT /note="Similar to M. tuberculosis Rv0011c, hypothetical FT protein, SW:Y011_MYCTU (P71581) (93 aa); Fasta score E(): FT 0, 89.2% identity in 93 aa overlap. Contains hydrophobic, FT possible membrane-spanning regions." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv0011c" FT RBS complement(17421..17424) FT /note="possible RBS" FT CDS 17483..18283 FT /class="III.C" FT /colour=0 FT /gene="ML0014" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv0012 (Best blastx score 411)" FT /product="possible cell division protein (pseudogene)" FT /pseudo FT CDS 18325..19023 FT /EC_number="4.1.3.-" FT /class="I.G.2" FT /colour=7 FT /gene="pabA" FT /gene="ML0015" FT /note="Similar to M. tuberculosis pabA, p-aminobenzoate FT synthase glutamine amidotransferase, TR:Q50183 FT (EMBL:Z70722) (232 aa); Fasta score E(): 0, 100.0% FT identity in 232 aa overlap (EMBL:AL123456) (232 aa); Fasta FT score E(): 0, 100.0% identity in 232 aa overlap and to FT Azospirillum brasilense trpG, anthranilate synthase FT component II, SW:TRPG_AZOBR (P26922) (196 aa); Fasta score FT E(): 0, 56.1% identity in 187 aa overlap. Previously FT sequenced as TR:Q50183. Contains Pfam match to entry FT PF00117 GATase, Glutamine amidotransferase class-I. FT Contains PS00442 Glutamine amidotransferases class-I FT active site." FT /product="putative p-aminobenzoate synthase glutamine FT amidotransferase" FT /tb_orthologue="pabA" FT misc_feature 18334..18900 FT /colour=0 FT /note="Pfam match to entry PF00117 GATase, Glutamine FT amidotransferase class-I, score 264.50, E-value 1.4e-75" FT misc_feature 18556..18591 FT /colour=8 FT /note="PS00442 Glutamine amidotransferases class-I active FT site" FT CDS complement(19001..20869) FT /EC_number="2.7.1.-" FT /class="I.J.3" FT /colour=9 FT /gene="pknB" FT /gene="ML0016" FT /note="Similar to M. tuberculosis pknB, serine/threonine FT protein kinase, SW:PKNB_MYCTU (P71584) (626 aa); Fasta FT score E(): 0, 86.3% identity in 626 aa overlap and to FT Streptomyces coelicolor pksC, serine/threonine protein FT kinase, TR:Q9ZFS8 (EMBL:AF094711) (556 aa); Fasta score FT E(): 0, 47.8% identity in 297 aa overlap. Previously FT sequenced as SW:PKNB_MYCLE (P54744) (622 aa); Fasta score FT E(): 0, 99.7% identity in 622 aa overlap. Contains Pfam FT match to entry PF00069 pkinase, Eukaryotic protein kinase FT domain. Contains PS00108 Serine/Threonine protein kinases FT active-site signature. Contains PS00107 Protein kinases FT ATP-binding region signature." FT /note="Similar to ML0017 and ML0897 and to part of ML0304" FT /product="putative serine/threonine protein kinase" FT /tb_orthologue="pknB" FT misc_feature complement(20054..20839) FT /colour=0 FT /note="Pfam match to entry PF00069 pkinase, Eukaryotic FT protein kinase domain, score 191.50, E-value 1.4e-53" FT misc_feature complement(20432..20470) FT /colour=8 FT /note="PS00108 Serine/Threonine protein kinases FT active-site signature" FT misc_feature complement(20750..20821) FT /colour=8 FT /note="PS00107 Protein kinases ATP-binding region FT signature" FT CDS complement(20879..22192) FT /EC_number="2.7.1.-" FT /class="I.J.3" FT /colour=9 FT /gene="pknA" FT /gene="ML0017" FT /note="Similar to M. tuberculosis pknA, serine/threonine FT protein kinase, SW:PKNA_MYCTU (P71585) (431 aa); Fasta FT score E(): 0, 81.0% identity in 431 aa overlap and to FT Streptomyces coelicolor pksC, serine/threonine protein FT kinase, TR:Q9ZFS8 (EMBL:AF094711) (556 aa); Fasta score FT E(): 2.1e-29, 39.9% identity in 346 aa overlap. Previously FT sequenced as SW:PKNA_MYCLE (P54743) (437 aa); Fasta score FT E(): 0, 84.6% identity in 448 aa overlap. Contains Pfam FT match to entry PF00069 pkinase, Eukaryotic protein kinase FT domain. Contains PS00108 Serine/Threonine protein kinases FT active-site signature." FT /note="Similar to ML0016 and ML0897 and to part of ML0304" FT /product="putative serine/threonine protein kinase" FT /tb_orthologue="pknA" FT misc_feature complement(21410..22156) FT /colour=0 FT /note="Pfam match to entry PF00069 pkinase, Eukaryotic FT protein kinase domain, score 187.90, E-value 1.6e-52" FT misc_feature complement(21746..21784) FT /colour=8 FT /note="PS00108 Serine/Threonine protein kinases FT active-site signature" FT CDS complement(22189..23667) FT /class="II.C.3" FT /colour=3 FT /gene="pbpA" FT /gene="ML0018" FT /note="Similar to M. tuberculosis pbpA, penicillin-binding FT protein, TR:P71586 (EMBL:AL123456) (491 aa); Fasta score FT E(): 0, 85.7% identity in 490 aa overlap. Also similar to FT Bacillus subtilis penicillin-binding protein 3, pbpC, FT SW:PBPC_BACSU (P42971) (668 aa); Fasta score E(): 2.8e-15, FT 24.5% identity in 444 aa overlap. Previously sequenced as FT TR:Q50185 (EMBL:Z70722) (474 aa); Fasta score E(): 0, FT 96.4% identity in 472 aa overlap. Contains a probable FT N-terminal signal sequence. Contains Pfam match to entry FT PF00905 Transpeptidase, Penicillin binding protein FT transpeptidase domain." FT /note="Similar to region of ML1577" FT /product="putative penicillin-binding protein" FT /tb_orthologue="pbpA" FT misc_feature complement(22222..23250) FT /colour=0 FT /note="Pfam match to entry PF00905 Transpeptidase, FT Penicillin binding protein transpeptidase domain, score FT 351.70, E-value 8.2e-102" FT CDS complement(23664..25061) FT /class="II.C.3" FT /colour=3 FT /gene="rodA" FT /gene="ML0019" FT /note="Similar to M. tuberculosis ftsW, cell-division FT protein, SW:FTSW_MYCTU (P71587) (469 aa); Fasta score E(): FT 0, 82.3% identity in 469 aa overlap and to Streptomyces FT coelicolor ftsW, cell-division protein, TR:Q9ZBA6 FT (EMBL:AL109663) (456 aa); Fasta score E(): 3.3e-25, 32.1% FT identity in 377 aa overlap. Previously sequenced as FT SW:FTSW_MYCLE (Q50186) (465 aa); Fasta score E(): 0, 99.6% FT identity in 465 aa overlap. Contains hydrophobic, possible FT membrane-spanning regions. Contains Pfam match to entry FT PF01098 FTSW_RODA_SPOVE, Cell cycle protein. Contains FT PS00428 Cell cycle proteins ftsW / rodA / spoVE FT signature." FT /note="Similar to ML0913" FT /product="putative cell-division protein" FT /tb_orthologue="rodA" FT misc_feature complement(23736..24830) FT /colour=0 FT /note="Pfam match to entry PF01098 FTSW_RODA_SPOVE, Cell FT cycle protein, score 563.80, E-value 1.1e-165" FT misc_feature complement(23796..23870) FT /colour=8 FT /note="PS00428 Cell cycle proteins ftsW / rodA / spoVE FT signature" FT CDS complement(25058..26587) FT /class="V" FT /colour=10 FT /gene="ML0020" FT /note="Similar to M. tuberculosis ppp, putative protein FT phosphatase, TR:P71588 (EMBL:AL123456) (514 aa); Fasta FT score E(): 0, 76.4% identity in 513 aa overlap and to FT Streptomyces coelicolor SCH69.15, possible protein FT phosphatase, TR:Q9XA19 (EMBL:AL079308) (515 aa); Fasta FT score E(): 0, 40.2% identity in 532 aa overlap. Also FT similar to many other proteins of undefined function. FT Previously sequenced as TR:Q50188 (EMBL:Z70722) (509 aa); FT Fasta score E(): 0, 99.6% identity in 509 aa overlap. FT Contains hydrophobic, possible membrane-spanning region. FT Proline-rich C-terminus." FT /product="conserved hypothetical protein" FT /tb_orthologue="ppp" FT CDS complement(26584..27051) FT /class="V" FT /colour=10 FT /gene="ML0021" FT /note="Similar to M. tuberculosis Rv0019c, hypothetical FT protein, TR:P71589 and to Streptomyces coelicolor FT SCH69.14, hypothetical protein, TR:Q9XA20 (EMBL:AL079308) FT (172 aa); Fasta score E(): 2e-21, 44.4% identity in 171 aa FT overlap. Previously sequenced as TR:Q50189 (EMBL:Z70722) FT (155 aa); Fasta score E(): 0, 100.0% identity in 155 aa FT overlap. Contains Pfam match to entry PF00498 FHA, FHA FT domain." FT /note="Similar at the C-terminus to ML2076" FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv0019c" FT misc_feature complement(26611..26805) FT /colour=0 FT /note="Pfam match to entry PF00498 FHA, FHA domain, score FT 74.50, E-value 2.2e-18" FT RBS complement(27058..27063) FT /note="possible RBS" FT CDS complement(27173..28639) FT /class="V" FT /colour=10 FT /gene="ML0022" FT /note="Similar to M. tuberculosis Rv0020c, hypothetical FT protein, TR:P71590 (EMBL:AL123456) (527 aa); Fasta score FT E(): 0, 71.9% identity in 531 aa overlap. Previously FT sequenced as TR:Q50190 (EMBL:Z70722) (463 aa); Fasta score FT E(): 0, 100.0% identity in 463 aa overlap. Contains Pfam FT match to entry PF00498 FHA, FHA domain." FT /note="Similar at the C-terminus to ML2076" FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv0020c" FT misc_feature complement(27203..27394) FT /colour=0 FT /note="Pfam match to entry PF00498 FHA, FHA domain, score FT 89.70, E-value 5.9e-23" FT tRNA 28890..28972 FT /colour=4 FT /note="tRNA Leu anticodon CAG, Cove score 63.58" FT CDS complement(28954..29265) FT /class="VI" FT /colour=8 FT /gene="ML0023" FT /ml_unique FT /note="Improbable CDS, possibly the remains of a disrupted FT gene." FT /product="hypothetical protein" FT CDS 29775..30026 FT /class="VI" FT /colour=8 FT /gene="ML0024" FT /ml_unique FT /note="Unknown function." FT /product="hypothetical protein" FT CDS complement(30293..30583) FT /class="VI" FT /colour=8 FT /gene="ML0025" FT /ml_unique FT /note="Unknown function." FT /product="hypothetical protein" FT CDS complement(31015..31651) FT /class="IV.C.2" FT /colour=0 FT /gene="PPE" FT /gene="ML0026" FT /note="Possible pseudogene of M. tuberculosis orthologue FT PPE (Best blastx score 99)" FT /product="PPE-family protein (pseudogene)" FT /pseudo FT CDS complement(32113..32920) FT /class="II.C.5" FT /colour=0 FT /gene="ML0027" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv0037c (Best blastx score 178)" FT /product="probable membrane protein (pseudogene)" FT /pseudo FT CDS 33100..33705 FT /class="V" FT /colour=10 FT /gene="ML0028" FT /note="Similar to M. tuberculosis Rv0038, hypothetical FT protein, TR:P71608 (EMBL:AL123456) (202 aa); Fasta score FT E(): 0, 88.1% identity in 202 aa overlap. Similar to FT Streptomyces coelicolor SCE59.07C, hypothetical protein, FT TR:CAB72194 (EMBL:AL138851) (193 aa); Fasta score E(): FT 3.5e-28, 45.6% identity in 182 aa overlap and shows weak FT similarity to many other bacterial hypothetical proteins. FT Previously sequenced as TR:Q50191 (EMBL:Z70722) (202 aa); FT Fasta score E(): 0, 100.0% identity in 201 aa overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv0038" FT CDS complement(34033..34638) FT /class="V" FT /colour=10 FT /gene="ML0029" FT /ml_unique FT /note="Contains a region similar to part of several FT bacterial hypothetical membrane proteins e.g. Streptomyces FT coelicolor SC4B5.06, putative transmembrane protein, FT TR:Q9ZBW2 (EMBL:AL034443) (345 aa); Fasta score E(): FT 2.4e-14, 54.1% identity in 109 aa overlap. The coding FT frame predicted by GC frameplot changes to either side of FT this region of similarity, suggesting that an ancestral FT CDS was disrupted. Given this, ML0029 may not be FT functional. Contains hydrophobic, possible FT membrane-spanning regions." FT /product="possible membrane protein" FT CDS complement(34750..35091) FT /class="II.C.4" FT /colour=3 FT /gene="ML0030" FT /note="Similar to M. tuberculosis Rv0039c, hypothetical FT protein, TR:P71696 (EMBL:AL123456) (115 aa); Fasta score FT E(): 2.2e-25, 63.2% identity in 114 aa overlap. Previously FT sequenced as TR:O32871 (EMBL:Z70722) (113 aa); Fasta score FT E(): 0, 100.0% identity in 113 aa overlap. Contains FT hydrophobic, possible membrane-spanning regions." FT /product="putative membrane protein" FT /tb_orthologue="Rv0039c" FT CDS complement(35287..36123) FT /class="II.C.2" FT /colour=3 FT /gene="ML0031" FT /note="Similar to M. tuberculosis MTC28, Rv0040c, proline FT rich 28 KDa antigen precursoR, SW:PR28_MYCTU (P71697) (311 FT aa); Fasta score E(): 0, 65.1% identity in 258 aa overlap. FT Previously sequenced as TR:O33075 (EMBL:Y14967) (278 aa); FT Fasta score E(): 0, 100.0% identity in 278 aa overlap. FT Contains a probable N-terminal signal sequence." FT /note="C-terminal half is similar to a region of ML0246" FT /tb_orthologue="Rv0040c" FT RBS complement(36133..36137) FT /note="possible RBS" FT CDS 36321..39239 FT /EC_number="6.1.1.4" FT /class="II.A.3" FT /colour=2 FT /gene="leuS" FT /gene="ML0032" FT /note="Similar to M. tuberculosis leuS, leucyl-tRNA FT synthetase, SW:SYL_MYCTU (P71698) (969 aa); Fasta score FT E(): 0, 83.6% identity in 972 aa overlap and to Bacillus FT subtilis leuS, leucyl-tRNA synthetase, SW:SYL_BACSU FT (P36430) (804 aa); Fasta score E(): 0, 45.7% identity in FT 941 aa overlap. Previously sequenced as SW:SYL_MYCLE FT (Q50192) (972 aa); Fasta score E(): 0, 99.9% identity in FT 972 aa overlap. Contains Pfam match to entry PF00133 FT tRNA-synt_1, tRNA synthetases class I (I, L, M and V). FT Contains PS00178 Aminoacyl-transfer RNA synthetases FT class-I signature." FT /product="leucyl-tRNA synthase" FT /tb_orthologue="leuS" FT misc_feature 36480..38816 FT /colour=0 FT /note="Pfam match to entry PF00133 tRNA-synt_1, tRNA FT synthetases class I (I, L, M and V), score 241.00, E-value FT 1.7e-68" FT misc_feature 36558..36590 FT /colour=8 FT /note="PS00178 Aminoacyl-transfer RNA synthetases class-I FT signature" FT repeat_region 39179..40156 FT CDS 39947..40211 FT /class="V" FT /colour=0 FT /gene="ML0033" FT /note="Possible pseudogene similar to M. tuberculosis FT paralogue Rv3906c (Best blastx score 95)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS 40825..41032 FT /class="V" FT /colour=0 FT /gene="ML0034" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3890c (Best blastx score 54)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS 42222..43083 FT /class="V" FT /colour=0 FT /gene="ML0035" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3888c (Best blastx score 182)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS 43199..43699 FT /class="II.C.4" FT /colour=0 FT /gene="ML0036" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3887c (Best blastx score 186)" FT /product="probable membrane protein (pseudogene)" FT /pseudo FT CDS 44077..45332 FT /class="II.B.3" FT /colour=0 FT /gene="ML0037" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3886c (Best blastx score 160)" FT /product="protease (pseudogene)" FT /pseudo FT CDS 45397..46827 FT /class="V" FT /colour=0 FT /gene="ML0038" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3885c (Best blastx score 128)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS 47271..48642 FT /class="V" FT /colour=0 FT /gene="ML0039" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3884c (Best blastx score 288)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT repeat_region 49168..50496 FT CDS complement(49689..49946) FT /note="Possible transposase remnant, similar to the FT N-terminus of M.tuberculosis Rv2424c, probable transposase FT for IS1558, TR:P71924" FT /blastp_match="TR:P71924 (EMBL:AL123456)" FT /class="IV.B.1.c" FT /colour=0 FT /gene="ML0040" FT /product="putative transposase remnant" FT /pseudo FT RBS 50644..50647 FT /note="possible RBS" FT CDS 50656..51996 FT /class="II.B.3" FT /colour=7 FT /gene="ML0041" FT /note="Similar to M. tuberculosis putative proteases e.g. FT Rv3883c, probable secreted protease, TR:O05461 FT (EMBL:AL123456) (446 aa); Fasta score E(): 0, 79.1% FT identity in 446 aa overlap. Shows weaker similarity to FT known bacterial proteases e.g. Bacteroides nodosus aprV5, FT acidic protease V5, TR:Q46541 (EMBL:L18984) (595 aa); FT Fasta score E(): 1.8e-07, 28.1% identity in 442 aa FT overlap. Previously sequenced as TR:O33076 (EMBL:Y14967) FT (446 aa); Fasta score E(): 0, 99.8% identity in 446 aa FT overlap. Contains a probable N-terminal signal sequence. FT Contains 2 Pfam matches to entry PF00082 Peptidase_S8, FT Subtilase family." FT /note="Similar to ML1538 and ML2528" FT /product="probable secreted protease" FT /tb_orthologue="Rv3883c" FT misc_feature 50860..51102 FT /colour=0 FT /note="Pfam match to entry PF00082 Peptidase_S8, Subtilase FT family, score 13.30, E-value 0.011" FT misc_feature 51184..51819 FT /colour=0 FT /note="Pfam match to entry PF00082 Peptidase_S8, Subtilase FT family, score 56.80, E-value 8.5e-15" FT CDS 51993..53396 FT /class="II.C.4" FT /colour=3 FT /gene="ML0042" FT /note="Similar to M. tuberculosis Rv3882c, conserved FT hypothetical protein, TR:O05462 (EMBL:AL123456) (462 aa); FT Fasta score E(): 0, 72.1% identity in 462 aa overlap. FT Shows weak similarity to M.tuberculosis Rv3885c, FT hypothetical protein, TR:O05459 (EMBL:AL123456) (537 aa); FT Fasta score E(): 3.1e-09, 28.0% identity in 382 aa FT overlap. Previously sequenced as TR:O33077 (EMBL:Y14967) FT (467 aa); Fasta score E(): 0, 100.0% identity in 467 aa FT overlap. Contains hydrophobic, possible membrane-spanning FT regions near the N-terminus." FT /note="Shows weak similarity to ML1537" FT /product="putative membrane protein" FT /tb_orthologue="Rv3882c" FT CDS 53719..54695 FT /class="V" FT /colour=0 FT /gene="ML0043" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3881c (Best blastx score 163)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT RBS 54684..54689 FT /note="possible RBS" FT CDS 54698..55039 FT /class="VI" FT /colour=10 FT /gene="ML0044" FT /note="Similar to M. tuberculosis Rv3880c, hypothetical FT protein, TR:O69744 (EMBL:AL123456) (115 aa); Fasta score FT E(): 2.6e-21, 56.4% identity in 110 aa overlap. Previously FT sequenced as TR:O33080 (EMBL:Y14967) (113 aa); Fasta score FT E(): 0, 100.0% identity in 113 aa overlap." FT CDS 55581..56699 FT /class="V" FT /colour=0 FT /gene="ML0045" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3879c (Best blastx score 189)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(57567..57922) FT /class="V" FT /colour=0 FT /gene="ML0046" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3878 (Best blastx score 118" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(58020..59558) FT /class="II.C.4" FT /colour=3 FT /gene="ML0047" FT /note="Similar to M. tuberculosis putative membrane FT protein e.g. Rv3877, hypothetical protein, TR:O69741 FT (EMBL:AL123456) (511 aa); Fasta score E(): 0, 74.0% FT identity in 512 aa overlap. Similarity is weaker to FT Rv1795, probable membrane protein, TR:O53944 FT (EMBL:AL123456) (503 aa); Fasta score E(): 2.1e-13, 22.7% FT identity in 519 aa overlap and Rv0290, hydrophobic FT protein, TR:O86362 (EMBL:AL123456) (472 aa); Fasta score FT E(): 2.4e-11, 26.6% identity in 512 aa overlap. Previously FT sequenced as TR:O33081 (EMBL:Y14967) (480 aa); Fasta score FT E(): 0, 100.0% identity in 480 aa overlap. Contains FT hydrophobic, possible membrane-spanning regions." FT /note="Similar to ML1539 and ML2529" FT /product="putative membrane protein" FT /tb_orthologue="Rv3877" FT CDS complement(59555..61315) FT /class="VI" FT /colour=10 FT /gene="ML0048" FT /note="Has a proline-rich N-terminus. Similar to several FT proteins from M. tuberculosis, some of which also contain FT proline-rich regions e.g. M.tuberculosis Rv3876, FT hypothetical protein, TR:O69740 (EMBL:AL123456) (666 aa); FT Fasta score E(): 0, 50.7% identity in 672 aa overlap, and FT Rv0530, hypothetical protein, TR:O06396 (EMBL:AL123456) FT (405 aa); Fasta score E(): 1.3e-19, 30.5% identity in 417 FT aa overlap. Similar to the C-terminal half of Streptomyces FT coelicolor SC3C3.03C, hypothetical protein, TR:O86637 FT (EMBL:AL031231) (1083 aa); Fasta score E(): 5.9e-27, 31.6% FT identity in 580 aa overlap, which contains Pro-Gln FT repeats. Previously sequenced as TR:O33082 (EMBL:Y14967) FT (478 aa); Fasta score E(): 0, 100.0% identity in 478 aa FT overlap." FT /note="C-terminal half is similar to that of ML0798" FT /product="conserved hypothetical protein" FT CDS complement(61406..61693) FT /class="II.C.2" FT /colour=3 FT /gene="ML0049" FT /note="Similar to M. tuberculosis ESAT6, Rv3875, 6 KDa FT early secretory antigenic target, SW:ESA6_MYCTU (Q57165) FT (94 aa); Fasta score E(): 4.2e-10, 36.3% identity in 91 aa FT overlap. Also shows weak similarity to others e.g. FT M.tuberculosis CFP7, Rv0288, 10 KDa antigen, SW:CFP7_MYCTU FT (O53693) (95 aa); Fasta score E(): 0.17, 25.6% identity in FT 78 aa overlap. Other members of this family include FT Rv3019c, hypothetical protein, TR:O53266 and Rv3444c, FT hypothetical protein, TR:O06261. Previously sequenced as FT SW:ESA6_MYCLE (Q50206) (95 aa); Fasta score E(): 0, 100.0% FT identity in 95 aa overlap." FT /product="possible secreted protein" FT RBS complement(61700..61703) FT /note="possible RBS" FT CDS complement(61720..62022) FT /class="II.C.2" FT /colour=3 FT /gene="ML0050" FT /note="Similar to M. tuberculosis lhp, Rv3874, culture FT filtrate protein 10 kDa CFP-10, TR:O69739 (EMBL:AF004671) FT (100 aa); Fasta score E(): 1.8e-10, 40.0% identity in 100 FT aa overlap. Also shows weak similarity to others e.g. M. FT tuberculosis Rv1197, hypothetical protein, TR:O05299 FT (EMBL:AL123456) (98 aa); Fasta score E(): 0.061, 21.9% FT identity in 96 aa overlap, and Rv1038c, hypothetical FT protein, TR:P96363 (EMBL:AL123456) (98 aa); Fasta score FT E(): 0.084, 21.9% identity in 96 aa overlap. These and FT others form a protein family typically found downstream of FT a member of the ESAT6 family. Other members include . FT Previously sequenced as TR:O33084 (EMBL:Y14967) (100 aa); FT Fasta score E(): 0, 100.0% identity in 100 aa overlap." FT /product="possible secreted protein" FT CDS complement(62201..63109) FT /class="IV.C.2" FT /colour=6 FT /gene="ML0051" FT /note="Similar to PPE-family proteins from M. tuberculosis FT e.g. Rv3873, PPE-family protein, TR:O69738 (EMBL:AL123456) FT (368 aa); Fasta score E(): 6.1e-32, 46.2% identity in 288 FT aa overlap. Lacks the PPE motif at the N-terminus. FT Previously sequenced as TR:O33085 (EMBL:Y14967) (302 aa); FT Fasta score E(): 0, 100.0% identity in 302 aa overlap. FT Contains Pfam match to entry PF00823 PPE, PPE family." FT /note="N-terminus is similar to the N-termini of ML0539, FT ML1828 and ML1991" FT /product="PPE-family protein" FT misc_feature complement(62720..63082) FT /colour=0 FT /note="Pfam match to entry PF00823 PPE, PPE family, score FT -37.40, E-value 1e-05" FT RBS complement(63118..63121) FT /note="possible RBS" FT CDS complement(63319..65112) FT /class="V" FT /colour=10 FT /gene="ML0052" FT /note="Similar to putative ATP/GTP-binding proteins from FT M. tuberculosis and Streptomyces coelicolor e.g. M. FT tuberculosis Rv3871, hypothetical protein, TR:O69736 FT (EMBL:AL123456) (591 aa); Fasta score E(): 0, 80.9% FT identity in 596 aa overlap. In several of these cases this FT protein is similar to the C-terminal half of a larger FT protein, while the upstream ML0053 is similar to the FT corresponding N-terminal half e.g. M. tuberculosis FT Rv3447c, unknown membrane protein, TR:O06264 FT (EMBL:AL123456) (1236 aa); Fasta score E(): 0, 35.2% FT identity in 588 aa overlap and Streptomyces coelicolor FT SC3C3.20C, ATP/GTP binding protein, TR:O86653 FT (EMBL:AL031231) (1321 aa); Fasta score E(): 0, 35.0% FT identity in 575 aa overlap. Related proteins are also FT found in Bacillus spp. Previously sequenced as TR:O33086 FT (EMBL:Y14967) (597 aa); Fasta score E(): 0, 99.7% identity FT in 597 aa overlap. Contains 2 Pfam matches to entry FT PF01580 FtsK_SpoIIIE, FtsK/SpoIIIE family. Contains 2 x FT PS00017 ATP/GTP-binding site motif A (P-loop)." FT /note="Similar to the C-terminal halves of ML1543 and FT ML2535" FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv3871" FT misc_feature complement(63559..64119) FT /colour=0 FT /note="Pfam match to entry PF01580 FtsK_SpoIIIE, FT FtsK/SpoIIIE family, score 178.70, E-value 9.4e-50" FT misc_feature complement(63961..63984) FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT misc_feature complement(64234..64992) FT /colour=0 FT /note="Pfam match to entry PF01580 FtsK_SpoIIIE, FT FtsK/SpoIIIE family, score 162.10, E-value 9.2e-45" FT misc_feature complement(64837..64860) FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT RBS complement(65124..65127) FT /note="possible RBS" FT CDS complement(65186..67420) FT /class="II.C.4" FT /colour=3 FT /gene="ML0053" FT /note="Similar to putative ATP/GTP-binding proteins from FT M. tuberculosis and Streptomyces coelicolor e.g. M. FT tuberculosis Rv3870, hypothetical protein, TR:O69735 FT (EMBL:AL123456) (747 aa); Fasta score E(): 0, 85.4% FT identity in 746 aa overlap. In several of these cases this FT protein is similar to the N-terminal half of a larger FT protein, while the downstream ML0052 is similar to the FT corresponding C-terminal half e.g. M.tuberculosis Rv3447c, FT unknown membrane protein, TR:O06264 (EMBL:AL123456) (1236 FT aa); Fasta score E(): 0, 37.2% identity in 732 aa overlap FT and Streptomyces coelicolor SC3C3.20C, ATP/GTP binding FT protein, TR:O86653 (EMBL:AL031231) (1321 aa); Fasta score FT E(): 0, 34.7% identity in 760 aa overlap. Related proteins FT are also found in Bacillus spp. Contains hydrophobic, FT possible membrane-spanning region near the N-terminus. FT Contains Pfam match to entry PF01580 FtsK_SpoIIIE, FT FtsK/SpoIIIE family. Contains PS00017 ATP/GTP-binding site FT motif A (P-loop)." FT /note="Similar to the N-terminal halves of ML1543 and FT ML2535" FT /product="putative membrane protein" FT /tb_orthologue="Rv3870" FT misc_feature complement(65513..66145) FT /colour=0 FT /note="Pfam match to entry PF01580 FtsK_SpoIIIE, FT FtsK/SpoIIIE family, score 250.40, E-value 2.4e-71" FT misc_feature complement(65969..65992) FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT CDS complement(67417..68862) FT /class="V" FT /colour=10 FT /gene="ML0054" FT /note="Similar to several M. tuberculosis hypothetical FT proteins e.g. Rv3869, conserved hypothetical protein, FT TR:O69734 (EMBL:AL123456) (480 aa); Fasta score E(): 0, FT 75.7% identity in 478 aa overlap. Previously sequenced as FT TR:O33088 (EMBL:Y14967) (481 aa); Fasta score E(): 0, FT 100.0% identity in 481 aa overlap. Contains hydrophobic, FT possible membrane-spanning region near the N-terminus." FT /note="Similar to ML1544 and ML2536" FT /product="putative membrane protein" FT /tb_orthologue="Rv3869" FT RBS complement(67428..67431) FT /note="possible RBS" FT CDS complement(68866..70587) FT /class="V" FT /colour=10 FT /gene="ML0055" FT /note="Similar to several M. tuberculosis hypothetical FT proteins e.g. Rv3868, hypothetical protein, SW:Y2G8_MYCTU FT (O69733) (573 aa); Fasta score E(): 0, 89.2% identity in FT 573 aa overlap. C-terminus is similar to many members of FT the CBXX/CFQX family e.g. Bacillus subtilis SpoVK/SpoVJ, FT stage V sporulation protein, SW:SP5K_BACSU (P27643) (322 FT aa); Fasta score E(): 3.5e-26, 33.7% identity in 332 aa FT overlap. Previously sequenced as SW:Y2G8_MYCLE (O33089) FT (573 aa); Fasta score E(): 0, 100.0% identity in 573 aa FT overlap. Contains Pfam match to entry PF00004 AAA, ATPases FT associated with various cellular activities (AAA). FT Contains PS00017 ATP/GTP-binding site motif A (P-loop)." FT /note="Similar to ML1536 and ML2537. Shows weak similarity FT to ML0798" FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv3868" FT misc_feature complement(68872..69603) FT /colour=0 FT /note="Pfam match to entry PF00004 AAA, ATPases associated FT with various cellular activities (AAA), score 15.30, FT E-value 1.7e-06" FT misc_feature complement(69565..69588) FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT CDS complement(70584..71093) FT /class="V" FT /colour=10 FT /gene="ML0056" FT /note="Similar to M. tuberculosis Rv3867, conserved FT hypothetical protein, TR:O06269 (EMBL:AL123456) (184 aa); FT Fasta score E(): 1.1e-12, 38.4% identity in 172 aa overlap FT and to Rv3614c, hypothetical protein, TR:O69732 FT (EMBL:AL123456) (183 aa); Fasta score E(): 1e-20, 48.2% FT identity in 166 aa overlap. Previously sequenced as FT TR:Q49730 (EMBL:U00015) (216 aa); Fasta score E(): FT 1.7e-13, 40.2% identity in 169 aa overlap." FT /note="Similar to ML0407" FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv3867" FT CDS complement(71142..72064) FT /class="V" FT /colour=0 FT /gene="ML0057" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3866 (Best blastx score 427)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT RBS complement(71605..71608) FT /note="possible RBS" FT CDS complement(72683..73686) FT /class="V" FT /colour=0 FT /gene="ML0058" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3864 (Best blastx score 110)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(73832..74366) FT /class="V" FT /colour=0 FT /gene="ML0059" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3863 (Best blastx score 119)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT repeat_region 74237..75125 FT CDS complement(76494..76713) FT /class="V" FT /colour=0 FT /gene="ML0060" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3860 (Best blastx score 354)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS 77709..82292 FT /EC_number="1.4.1.13" FT /class="I.D.1" FT /colour=7 FT /gene="gltB" FT /gene="ML0061" FT /note="Similar to M. tuberculosis Rv3859c, putative FT ferredoxin-dependent glutamate synthase, TR:P96218 FT (EMBL:Al123456) (1527 aa); Fasta score E(): 0, 90.2% FT identity in 1527 aa overlap and to Synechocystis sp. gltB, FT ferredoxin-dependent glutamate synthase 1, SW:GLTB_SYNY3 FT (P55037) (1550 aa); Fasta score E(): 0, 57.2% identity in FT 1525 aa overlap. Contains Pfam match to entry PF01493 FT DUF14, Domain of unknown function. Contains Pfam match to FT entry PF01645 Glu_synthase, Conserved region in glutamate FT synthase. Contains PS00095 C-5 cytosine-specific DNA FT methylases C-terminal signature." FT /product="putative ferredoxin-dependent glutamate FT synthase" FT /tb_orthologue="gltB" FT misc_feature 80163..81278 FT /colour=0 FT /note="Pfam match to entry PF01645 Glu_synthase, Conserved FT region in glutamate synthase, score 778.30, E-value FT 3.1e-230" FT misc_feature 81510..82103 FT /colour=0 FT /note="Pfam match to entry PF01493 DUF14, Domain of FT unknown function, score 324.10, E-value 1.6e-93" FT misc_feature 81627..81683 FT /colour=8 FT /note="PS00095 C-5 cytosine-specific DNA methylases FT C-terminal signature" FT RBS 82274..82277 FT /note="possible RBS" FT CDS 82285..83751 FT /EC_number="1.4.1.13" FT /class="I.D.1" FT /colour=7 FT /gene="gltD" FT /gene="ML0062" FT /note="Similar to M. tuberculosis Rv3858c, gltD, putative FT NADH-dependent glutamate synthase small subunit, TR:P96219 FT (EMBL:Al123456) (488 aa); Fasta score E(): 0, 87.7% FT identity in 488 aa overlap and to many other putative FT glutamate synthase small subunits e.g. Escherichia coli FT gltD, glutamate synthase [NADPH] small chain, FT SW:GLTD_ECOLI (P09832) (471 aa); Fasta score E(): 0, 37.8% FT identity in 474 aa overlap. Contains Pfam match to entry FT PF00070 pyr_redox, Pyridine nucleotide-disulphide FT oxidoreductase." FT /product="NADH-dependent glutamate synthase small subunit" FT /tb_orthologue="gltD" FT misc_feature 82723..83619 FT /colour=0 FT /note="Pfam match to entry PF00070 pyr_redox, Pyridine FT nucleotide-disulphide oxidoreductase, score 114.20, FT E-value 6e-33" FT CDS 84980..85727 FT /class="V" FT /colour=0 FT /gene="ML0063" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3856c (Best blastx score 211)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(85968..86612) FT /class="I.J.1" FT /colour=9 FT /gene="ML0064" FT /note="Similar to M. tuberculosis Rv3855, putative FT transcriptional regulator, TR:P96222 (EMBL:Al123456) (216 FT aa); Fasta score E(): 0, 77.0% identity in 213 aa overlap. FT Contains a probable helix-turn-helix motif at aa 45-66 FT (Score 1261, SD +3.48) Contains Pfam match to entry FT PF00440 tetR, Bacterial regulatory proteins, tetR family." FT /note="Similar to ML1070" FT /product="putative transcriptional regulator" FT /tb_orthologue="Rv3855" FT misc_feature complement(86388..86528) FT /colour=0 FT /note="Pfam match to entry PF00440 tetR, Bacterial FT regulatory proteins, tetR family, score 56.20, E-value FT 5.6e-13" FT CDS 86681..88165 FT /class="I.B.7" FT /colour=7 FT /gene="ML0065" FT /note="Similar to putative monooxygenases from M. FT tuberculosis e.g. Rv3854c, probable monooxygenase, FT TR:P96223 (EMBL:AL123456) (489 aa); Fasta score E(): 0, FT 80.1% identity in 488 aa overlap. Others are Rv0892 and FT Rv1393c. Also similar to the C-terminal half of FT Deinococcus radiodurans plasmid MP1 DRB0033, putative FT arylesterase/monoxygenase, TR:Q9RZT0 (EMBL:AE001826) (833 FT aa); Fasta score E(): 0, 51.5% identity in 480 aa overlap. FT Shows weaker similarity to Rhodococcus rhodochrous steroid FT monooxygenase, TR:O50641 (EMBL:AB010439) (549 aa); Fasta FT score E(): 2e-15, 24.9% identity in 469 aa overlap. FT Contains Pfam match to entry PF00743 FMO-like, FT Flavin-binding monooxygenase-like." FT /note="Similar to pseudogene ML1732" FT /product="putative monooxygenase" FT /tb_orthologue="Rv3854c" FT misc_feature 86714..88111 FT /colour=0 FT /note="Pfam match to entry PF00743 FMO-like, FT Flavin-binding monooxygenase-like, score -279.10, E-value FT 1.7e-07" FT CDS complement(88246..88719) FT /class="I.G.11" FT /colour=7 FT /gene="menG" FT /gene="ML0066" FT /note="Similar to M. tuberculosis Rv3853, menG, putative FT S-adenosylmethionine:2-demethylmenaquinone FT methyltransferase, TR:P96224 (EMBL:AL123456) (157 aa); FT Fasta score E(): 0, 87.1% identity in 155 aa overlap. FT Similar to Escherichia coli menG, FT S-adenosylmethionine:2-demethylmenaquinone FT methyltransferase, SW:MENG_ECOLI (P32165) (161 aa); Fasta FT score E(): 2.4e-20, 45.6% identity in 149 aa overlap." FT /product="putative FT S-adenosylmethionine:2-demethylmenaquinone FT methyltransferase" FT /tb_orthologue="menG" FT CDS complement(88741..89106) FT /class="II.A.4" FT /colour=2 FT /gene="hns" FT /gene="ML0067" FT /note="Similar to M. tuberculosis Rv3852, hns, HU-histone FT protein, TR:P96225 (EMBL:AL123456) (134 aa); Fasta score FT E(): 6.1e-12, 51.5% identity in 134 aa overlap. Contains FT PAKKX repeats similar to those in histone H1 proteins." FT /product="histone-like protein" FT /tb_orthologue="hns" FT RBS complement(89114..89118) FT /note="possible RBS" FT CDS complement(89620..90336) FT /class="V" FT /colour=10 FT /gene="ML0068" FT /note="Similar to M. tuberculosis Rv3850, hypothetical FT protein, TR:P96227 (EMBL:AL123456) (218 aa); Fasta score FT E(): 0, 78.3% identity in 217 aa overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv3850" FT RBS complement(90347..90351) FT /note="possible RBS" FT CDS complement(90521..90919) FT /class="V" FT /colour=10 FT /gene="ML0069" FT /note="Similar to M. tuberculosis Rv3849, hypothetical FT protein, TR:P96228 (EMBL:AL123456) (132 aa); Fasta score FT E(): 0, 84.0% identity in 131 aa overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv3849" FT RBS complement(90927..90932) FT /note="possible RBS" FT CDS complement(91296..91559) FT /class="VI" FT /colour=8 FT /gene="ML0070" FT /ml_unique FT /note="Unknown function." FT /product="hypothetical protein" FT CDS complement(91913..92446) FT /class="V" FT /colour=10 FT /gene="ML0071" FT /note="Similar to M. tuberculosis Rv3847, hypothetical FT protein, TR:P96230 (EMBL:AL123456) (177 aa); Fasta score FT E(): 0, 96.6% identity in 177 aa overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv3847" FT RBS complement(92452..92456) FT /note="possible RBS" FT CDS complement(92647..93270) FT /EC_number="1.15.1.1" FT /class="III.F" FT /colour=0 FT /gene="sodA" FT /gene="ML0072" FT /note="Similar to M. tuberculosis Rv3846, sodA, superoxide FT dismutase, SW:SODF_MYCTU (P17670) (207 aa); Fasta score FT E(): 0, 80.1% identity in 206 aa overlap, and to many FT other mycobacterial superoxide dismutases. Previously FT sequenced as SW:SODM_MYCLE (P13367) (206 aa); Fasta score FT E(): 0, 100.0% identity in 206 aa overlap. Contains Pfam FT match to entry PF00081 sodfe, Iron/manganese superoxide FT dismutases (SODM). Contains PS00088 Manganese and iron FT superoxide dismutases signature." FT /product="superoxide dismutase" FT /tb_orthologue="sodA" FT misc_feature complement(92674..93264) FT /colour=0 FT /note="Pfam match to entry PF00081 sodfe, Iron/manganese FT superoxide dismutases (SODM), score 427.70, E-value FT 1e-124" FT misc_feature complement(92770..92793) FT /colour=8 FT /note="PS00088 Manganese and iron superoxide dismutases FT signature" FT RBS complement(93277..93285) FT /note="possible RBS" FT CDS 94092..95126 FT /class="II.C.4" FT /colour=3 FT /gene="ML0073" FT /note="Similar to M. tuberculosis Rv3843c, putative FT membrane protein, TR:P96235 (EMBL:Z83864) (342 aa); Fasta FT score E(): 0, 63.0% identity in 349 aa overlap. Contains FT hydrophobic, possible membrane-spanning regions." FT /product="putative membrane protein" FT /tb_orthologue="Rv3843c" FT CDS 95132..95947 FT /EC_number="3.1.4.46" FT /class="I.C.1" FT /colour=7 FT /gene="glpQ" FT /gene="ML0074" FT /note="Similar to M. tuberculosis Rv3842c, glpQ1, FT glycerophosphoryl diester phosphodiesterase, TR:P96236 FT (EMBL:AL123456) (274 aa); Fasta score E(): 0, 88.8% FT identity in 269 aa overlap. Similar to Bacillus subtilis FT glpQ, glycerophosphoryl diester phosphodiesterase, FT SW:GLPQ_BACSU (P37965) (293 aa); Fasta score E(): 2.3e-20, FT 32.0% identity in 250 aa overlap." FT /product="putative glycerophosphoryl diester FT phosphodiesterase" FT /tb_orthologue="glpQ1" FT CDS complement(95965..96439) FT /class="I.G.14" FT /colour=0 FT /gene="bfrB" FT /gene="ML0075" FT /note="Possible pseudogene of M. tuberculosis orthologue FT bfrB (Best blastx score 190)" FT /product="bacterioferritin (pseudogene)" FT /pseudo FT CDS complement(97037..97282) FT /colour=0 FT /gene="ML0076" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3840 (Best blastx score 196)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(97832..98119) FT /class="V" FT /colour=0 FT /gene="ML0077" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3839 (Best blastx score 251)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS 98425..99393 FT /EC_number="4.2.1.51" FT /class="I.D.4" FT /colour=7 FT /gene="pheA" FT /gene="ML0078" FT /note="Similar to M. tuberculosis Rv3838c, pheA, FT prephenate dehydratase, TR:P96240 (EMBL:AL123456) (321 FT aa); Fasta score E(): 0, 83.9% identity in 311 aa overlap. FT Similar to Amycolatopsis methanolica pheA, prephenate FT dehydratase, SW:PHEA_AMYME (Q44104) (304 aa); Fasta score FT E(): 0, 44.4% identity in 315 aa overlap. Contains Pfam FT match to entry PF01842 ACT, ACT domain. Contains Pfam FT match to entry PF00800 PDT, Prephenate dehydratase. FT Contains PS00858 Prephenate dehydratase signature 2." FT /product="putative prephenate dehydratase" FT /tb_orthologue="pheA" FT misc_feature 98440..99006 FT /colour=0 FT /note="Pfam match to entry PF00800 PDT, Prephenate FT dehydratase, score 313.20, E-value 3e-90" FT misc_feature 99034..99267 FT /colour=0 FT /note="Pfam match to entry PF01842 ACT, ACT domain, score FT 55.20, E-value 1.4e-12" FT misc_feature 99115..99138 FT /colour=8 FT /note="PS00858 Prephenate dehydratase signature 2" FT CDS 99390..100085 FT /class="I.B.1" FT /colour=7 FT /gene="ML0079" FT /note="Similar to M. tuberculosis Rv3837c, putative FT phosphoglycerate mutase, TR:P96241 (EMBL:AL123456) (232 FT aa); Fasta score E(): 0, 71.6% identity in 232 aa overlap. FT Similar to Amycolatopsis methanolica pgm, 2,3-PDG FT dependent phosphoglycerate mutase, TR:Q9ZAX0 (EMBL:U73808) FT (205 aa); Fasta score E(): 9.3e-24, 39.7% identity in 204 FT aa overlap. Contains Pfam match to entry PF00300 PGAM, FT Phosphoglycerate mutase family. Contains PS00175 FT Phosphoglycerate mutase family phosphohistidine FT signature." FT /note="Similar to ML1452" FT /product="putative phosphoglycerate mutase" FT /tb_orthologue="Rv3837c" FT misc_feature 99405..99869 FT /colour=0 FT /note="Pfam match to entry PF00300 PGAM, Phosphoglycerate FT mutase family, score 27.40, E-value 2e-06" FT misc_feature 99408..99437 FT /colour=8 FT /note="PS00175 Phosphoglycerate mutase family FT phosphohistidine signature" FT CDS complement(100374..100651) FT /class="V" FT /colour=0 FT /gene="ML0080" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3836 (Best blastx score 137)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(100648..102000) FT /class="II.C.4" FT /colour=3 FT /gene="ML0081" FT /note="Similar to M. tuberculosis Rv3835c, hypothetical FT protein, TR:P96243 (EMBL:AL123456) (449 aa); Fasta score FT E(): 0, 69.4% identity in 457 aa overlap. Contains FT hydrophobic, possible membrane-spanning region near the FT N-terminus." FT /product="putative membrane protein" FT /tb_orthologue="Rv3835" FT CDS 102122..103375 FT /EC_number="6.1.1.11" FT /class="II.A.3" FT /colour=2 FT /gene="serS" FT /gene="ML0082" FT /note="Similar to M. tuberculosis Rv3834c, serS, FT seryl-tRNA synthetase, SW:SYS_MYCTU (P96244) (419 aa); FT Fasta score E(): 0, 85.8% identity in 416 aa overlap. FT Similar to Staphylococcus aureus serS, seryl-tRNA FT synthetase, SW:SYS_STAAU (Y09924) (428 aa); Fasta score FT E(): 0, 36.8% identity in 418 aa overlap. Contains Pfam FT match to entry PF00587 tRNA-synt_2b, tRNA synthetase class FT II (G, H, P, S and T). Contains PS00179 Aminoacyl-transfer FT RNA synthetases class-II signature 1." FT /product="putative seryl-tRNA synthase" FT /tb_orthologue="serS" FT misc_feature 102338..103348 FT /colour=0 FT /note="Pfam match to entry PF00587 tRNA-synt_2b, tRNA FT synthetase class II (G, H, P, S and T), score 132.30, FT E-value 8.8e-36" FT misc_feature 102881..102955 FT /colour=8 FT /note="PS00179 Aminoacyl-transfer RNA synthetases class-II FT signature 1" FT CDS complement(105732..106191) FT /class="V" FT /colour=0 FT /gene="ML0083" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3831 (Best blastx score 151)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(106286..106599) FT /class="V" FT /colour=0 FT /gene="ML0084" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3819 (Best blastx score 138)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(106635..108159) FT /class="V" FT /colour=0 FT /gene="ML0085" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3818 (Best blastx score 275)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(108258..108991) FT /class="IV.D" FT /colour=0 FT /gene="ML0086" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3817 (Best blastx score 203)" FT /product="probable aminoglycoside 3'-phosphotransferase FT (pseudogene)" FT /pseudo FT CDS 109049..109822 FT /class="IV.H" FT /colour=7 FT /gene="ML0087" FT /note="Similar to M. tuberculosis Rv3816c, probable FT acyltransferase, TR:O07807 (EMBL:AL123456) (259 aa); Fasta FT score E(): 0, 81.5% identity in 254 aa overlap. Also FT similar to the neighbouring putative acyltransferases in FT M. tuberculosis, Rv3815c and Rv3814c. Shows similarity to FT many putative bacterial acyltransferases. Contains Pfam FT match to entry PF01553 Acyltransferase, Acyltransferase." FT /note="Similar to ML0892" FT /product="putative acyltransferase" FT /tb_orthologue="Rv3816c" FT misc_feature 109136..109678 FT /colour=0 FT /note="Pfam match to entry PF01553 Acyltransferase, FT Acyltransferase, score 170.90, E-value 2.1e-47" FT CDS 109842..110234 FT /class="IV.H" FT /colour=0 FT /gene="ML0088" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3815c (Best blastx score 228)" FT /product="probable acyltransferase (pseudogene)" FT /pseudo FT CDS 110329..111174 FT /class="V" FT /colour=10 FT /gene="ML0089" FT /note="Similar to M. tuberculosis Rv3813c, conserved FT hypothetical protein, TR:O07810 (EMBL:AL123456) (273 aa); FT Fasta score E(): 0, 80.4% identity in 271 aa overlap. FT Similar to many other bacterial hypothetical proteins. FT Contains Pfam match to entry PF00592 DUF3, Cof family." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv3813c" FT misc_feature 110401..111135 FT /colour=0 FT /note="Pfam match to entry PF00592 DUF3, Cof family, score FT 91.10, E-value 2.2e-23" FT CDS complement(111660..112884) FT /class="II.C.2" FT /colour=0 FT /gene="csp" FT /gene="ML0090" FT /note="Possible pseudogene of M. tuberculosis orthologue FT csp (Best blastx score 228)" FT /product="secreted protein (pseudogene)" FT /pseudo FT CDS complement(113153..113863) FT /class="II.C.2" FT /colour=3 FT /gene="ML0091" FT /note="Similar to M. tuberculosis Rv3810, erp, pirG, FT exported repetitive protein precursor, SW:ERP_MYCTU FT (Q50793) (284 aa); Fasta score E(): 1.8e-23, 52.7% FT identity in 281 aa overlap. Previously sequenced as FT SW:28KD_MYCLE (P19361) (236 aa); Fasta score E(): 0, 99.6% FT identity in 236 aa overlap. Contains a probable N-terminal FT signal sequence." FT /product="28 KDa antigen precursor" FT RBS complement(113870..113874) FT /note="possible RBS" FT CDS 114130..115371 FT /class="II.C.3" FT /colour=3 FT /gene="glf" FT /gene="ML0092" FT /note="Similar to M. tuberculosis Rv3809c, glf, FT UDP-galactopyranose mutase, TR:O06934 (EMBL:U96128) (399 FT aa); Fasta score E(): 0, 86.6% identity in 396 aa overlap. FT Similar to e.g. Escherichia coli glf, UDP-galactopyranose FT mutase, SW:GLF_ECOLI (P37747) (367 aa); Fasta score E(): FT 0, 43.1% identity in 378 aa overlap." FT /product="putative UDP-galactopyranose mutase" FT /tb_orthologue="glf" FT CDS 115371..117302 FT /class="V" FT /colour=10 FT /gene="ML0093" FT /note="Similar to M. tuberculosis Rv3808c, hypothetical FT protein, TR:O53585 (EMBL:AL123456) (637 aa); Fasta score FT E(): 0, 85.4% identity in 643 aa overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv3808c" FT CDS 117295..117873 FT /class="II.C.4" FT /colour=3 FT /gene="ML0094" FT /note="Similar to M. tuberculosis Rv3807c, possible FT membrane protein, TR:O53584 (EMBL:AL123456) (165 aa); FT Fasta score E(): 0, 72.8% identity in 151 aa overlap and FT to Streptomyces coelicolor SC5G8.11, putative integral FT membrane protein, TR:CAB89062 (EMBL:AL353872) (169 aa); FT Fasta score E(): 1.7e-15, 45.1% identity in 153 aa FT overlap. Contains hydrophobic, possible membrane-spanning FT region. Contains Pfam match to entry PF01569 PAP2, PAP2 FT superfamily." FT /product="putative membrane protein" FT /tb_orthologue="Rv3807c" FT misc_feature 117409..117825 FT /colour=0 FT /note="Pfam match to entry PF01569 PAP2, PAP2 superfamily, FT score 97.10, E-value 3.4e-25" FT CDS 117870..118778 FT /class="II.C.4" FT /colour=3 FT /gene="ML0095" FT /note="Similar to M. tuberculosis Rv3806c, possible FT integral membrane protein, TR:O53583 (EMBL:AL123456) (302 FT aa); Fasta score E(): 0, 83.8% identity in 302 aa overlap FT and to bacterial putative membrane proteins e.g. FT Streptomyces coelicolor SC5G8.12, putative integral FT membrane protein, TR:CAB89063 (EMBL:AL353872) (322 aa); FT Fasta score E(): 0, 48.6% identity in 290 aa overlap and FT Azorhizobium caulinodans noeC, possible integral membrane FT protein found near nod locus, SW:NOEC_AZOCA (Q43967) (307 FT aa); Fasta score E(): 1.1e-12, 31.4% identity in 191 aa FT overlap. Contains hydrophobic, possible membrane-spanning FT regions." FT /product="putative integral membrane protein" FT /tb_orthologue="Rv3806c" FT CDS 118819..120768 FT /class="II.C.4" FT /colour=3 FT /gene="ML0096" FT /note="similar to M. tuberculosis Rv3805c, possible FT membrane protein, TR:O53582 (EMBL:AL123456) (627 aa); FT Fasta score E(): 0, 80.9% identity in 629 aa overlap. FT Contains hydrophobic, possible membrane-spanning regions." FT /product="putative membrane protein" FT /tb_orthologue="Rv3805c" FT RBS 121138..121145 FT /note="possible RBS" FT CDS 121157..122149 FT /class="I.H.3" FT /colour=1 FT /gene="fbpA" FT /gene="ML0097" FT /note="Similar to mycobacterial fbpA, antigen 85-A FT precursors e.g. M. tuberculosis fbpA, antigen 85-A FT precursor, SW:A85A_MYCTU (P17944) (338 aa); Fasta score FT E(): 0, 83.0% identity in 329 aa overlap. Also similar to FT the B- and C- proteins of the mycobacterial antigen 85 FT complexes. Similar to N-terminal region of Corynebacterium FT glutamicum csp1, PS1 protein precursor, SW:CSP1_CORGL FT (Q01377) (657 aa); Fasta score E(): 5.2e-31, 38.6% FT identity in 290 aa overlap. Previously sequenced as FT SW:A85A_MYCLE (Q05861) (330 aa); Fasta score E(): 0, FT 100.0% identity in 330 aa overlap. Contains probable FT N-terminal signal sequence. Contains Pfam match to entry FT PF00756 Esterase, Putative esterase." FT /note="Similar to ML0098, ML2028 and ML2655" FT /product="antigen 85A, mycolytransferase" FT /tb_orthologue="fbpA" FT misc_feature 121241..121903 FT /colour=0 FT /note="Pfam match to entry PF00756 Esterase, Putative FT esterase, score 381.30, E-value 9.7e-111" FT RBS 122299..122304 FT /note="possible RBS" FT CDS 122310..123215 FT /class="I.H.3" FT /colour=1 FT /gene="fbpC" FT /gene="ML0098" FT /note="Similar to mycobacterial fbpC, antigen 85-C FT precursors e.g. M. tuberculosis fbpC, antigen 85-C FT precursor, SW:A85C_MYCTU (P17944) (340 aa); Fasta score FT E(): 0, 40.7% identity in 305 aa overlap. Also similar to FT the B- and C- proteins of the mycobacterial antigen 85 FT complexes. Similar to N-terminal region of Corynebacterium FT glutamicum csp1, PS1 protein precursor, SW:CSP1_CORGL FT (Q01377) (657 aa); Fasta score E(): 1.4e-14, 31.5% FT identity in 248 aa overlap. Previously sequenced as MPB51 FT precursor, TR:Q48923 (EMBL:D26486) (299 aa); Fasta score FT E(): 0, 77.5% identity in 302 aa overlap. Contains FT probable N-terminal signal sequence. Contains Pfam match FT to entry PF00756 Esterase, Putative esterase." FT /note="Similar to ML0097, ML2028 and ML2655" FT /product="antigen 85C, mycolyltransferase" FT /tb_orthologue="fbpC1" FT misc_feature 122382..122996 FT /colour=0 FT /note="Pfam match to entry PF00756 Esterase, Putative FT esterase, score 305.30, E-value 7.3e-88" FT RBS 123358..123363 FT /note="possible RBS" FT CDS 123370..124380 FT /class="V" FT /colour=10 FT /gene="ML0099" FT /note="Similar to M. tuberculosis Rv3802c, hypothetical FT protein, TR:O53581 (EMBL:AL123456) (336 aa); Fasta score FT E(): 0, 75.5% identity in 335 aa overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv3802c" FT CDS 124599..126506 FT /class="I.A.3" FT /colour=1 FT /gene="fadD32" FT /gene="ML0100" FT /note="Similar to several putative acyl-CoA synthases from FT M. tuberculosis e.g. M. tuberculosis Rv3801c, fadD32, FT putative acyl-CoA synthase, TR:O53580 (EMBL:AL123456) (637 FT aa); Fasta score E(): 0, 93.2% identity in 632 aa overlap. FT Similar to Mycobacterium bovis BCG ORF3, acyl-CoA FT synthase, TR:P71495 (EMBL:U75685) (582 aa); Fasta score FT E(): 0, 36.8% identity in 581 aa overlap and to domains of FT polyketide synthases e.g. Myxococcus xanthus safB, FT saframycin Mx1 synthetase B, TR:Q50857 (EMBL:U24657) (1770 FT aa); Fasta score E(): 0, 36.4% identity in 605 aa overlap. FT Contains Pfam match to entry PF00501 AMP-binding, FT AMP-binding enzyme." FT /note="Similar to ML0132, ML0138, ML1234 and ML2358" FT /product="putative acyl-CoA synthetase" FT /tb_orthologue="fadD32" FT misc_feature 124803..126158 FT /colour=0 FT /note="Pfam match to entry PF00501 AMP-binding, FT AMP-binding enzyme, score -85.00, E-value 1.1e-10" FT CDS 126518..131872 FT /class="I.I" FT /colour=1 FT /gene="pks13" FT /gene="ML0101" FT /note="Similar to many polyketide synthases from M. FT tuberculosis e.g. ppsA, M. tuberculosis phenolpthiocerol FT synthesis polyketide synthase, SW:PPSA_MYCTU (Q10977) FT (1876 aa); Fasta score E(): 0, 36.1% identity in 1277 aa FT overlap. Similar to many others e.g. Streptomyces noursei FT nysI, nystatin biosynthesis polyketide synthase, FT TR:AAF71766 (EMBL:AF263912) (9477 aa); Fasta score E(): 0, FT 33.4% identity in 1215 aa overlap. Contains Pfam match to FT entry PF00109 ketoacyl-synt, Beta-ketoacyl synthase. FT Contains Pfam match to entry PF00698 Acyl_transf, Acyl FT transferase domain. Contains Pfam match to entry PF00975 FT Thioesterase, Thioesterase domain. Contains 2 Pfam matches FT to entry PF00550 pp-binding, Phosphopantetheine attachment FT site. Contains PS00606 Beta-ketoacyl synthases active FT site." FT /note="Similar to ML0135, ML0139, ML1229, ML2353, ML2354, FT ML2355, ML2356, ML2357, and ML2607" FT /product="polyketide synthase" FT /tb_orthologue="pks13" FT misc_feature 126689..126889 FT /colour=0 FT /note="Pfam match to entry PF00550 pp-binding, FT Phosphopantetheine attachment site, score 55.80, E-value FT 8.4e-14" FT misc_feature 126971..128248 FT /colour=0 FT /note="Pfam match to entry PF00109 ketoacyl-synt, FT Beta-ketoacyl synthase, score 596.90, E-value 1.3e-175" FT misc_feature 127448..127498 FT /colour=8 FT /note="PS00606 Beta-ketoacyl synthases active site" FT misc_feature 128747..129730 FT /colour=0 FT /note="Pfam match to entry PF00698 Acyl_transf, Acyl FT transferase domain, score 213.30, E-value 3.7e-60" FT misc_feature 130316..130456 FT /colour=0 FT /note="Pfam match to entry PF00550 pp-binding, FT Phosphopantetheine attachment site, score 23.60, E-value FT 6.6e-05" FT misc_feature 131084..131836 FT /colour=0 FT /note="Pfam match to entry PF00975 Thioesterase, FT Thioesterase domain, score 40.90, E-value 2.8e-08" FT CDS 131869..133422 FT /class="I.H.1" FT /colour=1 FT /gene="accD4" FT /gene="ML0102" FT /note="Similar to many acyl-CoA carboxylase subunits e.g. FT M. tuberculosis pccB, acetyl/propionyl-CoA carboxylase FT [beta] subunit, TR:O53578 (EMBL:AL123456) (518 aa); Fasta FT score E(): 0, 91.2% identity in 512 aa overlap and FT Streptomyces coelicolor pccB, propionyl-CoA carboxylase FT complex B subunit, TR:Q9X4K7 (EMBL:AF113605) (530 aa); FT Fasta score E(): 0, 48.2% identity in 521 aa overlap. FT Contains Pfam match to entry PF01039 Carboxyl_trans, FT Carboxyl transferase domain." FT /note="Similar to ML0731 and ML1657" FT /product="putative acyl CoA carboxylase [beta] subunit" FT /tb_orthologue="accD4" FT misc_feature 131914..133419 FT /colour=0 FT /note="Pfam match to entry PF01039 Carboxyl_trans, FT Carboxyl transferase domain, score 544.70, E-value FT 6.3e-160" FT CDS complement(133849..135036) FT /class="I.A.3" FT /colour=0 FT /gene="fadE35" FT /gene="ML0103" FT /note="Possible pseudogene of M. tuberculosis orthologue FT fadE35 (Best blastx score 159)" FT /product="acyl-CoA dehydrogenase (pseudogene)" FT /pseudo FT CDS complement(136573..139824) FT /class="II.C.2" FT /colour=3 FT /gene="embB" FT /gene="ML0104" FT /note="Similar to M. tuberculosis embB, probable FT arabinosyl transferase, TR:P72030 (EMBL:AL123456) (1098 FT aa); Fasta score E(): 0, 78.0% identity in 1101 aa overlap FT and to other putative mycobacterial FT arabinosyltransferases. Involved in resistance to FT ethambutol in M. tuberculosis. Contains hydrophobic, FT possible membrane-spanning regions." FT /note="Similar to ML0105 and ML0106" FT /product="putative arabinosyl transferase" FT /tb_orthologue="embB" FT CDS complement(139821..143156) FT /class="II.C.2" FT /colour=3 FT /gene="embA" FT /gene="ML0105" FT /note="Similar to M. tuberculosis embA, probable FT arabinosyl transferase, TR:Q50394 (EMBL:AL123456) (1092 FT aa); Fasta score E(): 0, 64.2% identity in 1108 aa overlap FT and to other putative mycobacterial FT arabinosyltransferases. Involved in resistance to FT ethambutol in M. tuberculosis. Contains hydrophobic, FT possible membrane-spanning regions." FT /note="Similar to ML0104 and ML0106" FT /product="putative arabinosyl transferase" FT /tb_orthologue="embA" FT CDS complement(144115..147327) FT /class="II.C.2" FT /colour=3 FT /gene="embC" FT /gene="ML0106" FT /note="Similar to M. tuberculosis embC, probable FT arabinosyl transferase, TR:P72059 (EMBL:AL123456) (1094 FT aa); Fasta score E(): 0, 82.8% identity in 1075 aa overlap FT and to other putative mycobacterial FT arabinosyltransferases. Involved in resistance to FT ethambutol in M. tuberculosis. Contains hydrophobic, FT possible membrane-spanning regions." FT /note="Similar to ML0104 and ML0105" FT /product="putative arabinosyl transferase" FT /tb_orthologue="embC" FT CDS complement(147460..149358) FT /class="II.C.4" FT /colour=3 FT /gene="ML0107" FT /note="Similar to M. tuberculosis Rv3792, unknown membrane FT protein, TR:P72058 (EMBL:AL123456) (643 aa); Fasta score FT E(): 0, 77.5% identity in 631 aa overlap and to the FT C-terminal half of Mycobacterium smegmatis ORF3, FT hypothetical membrane protein, TR:O05765 (EMBL:U46844) FT (603 aa); Fasta score E(): 0, 67.8% identity in 267 aa FT overlap. Contains hydrophobic, possible membrane-spanning FT regions." FT /product="putative membrane protein" FT /tb_orthologue="Rv3792" FT CDS complement(149361..150125) FT /class="I.B.7" FT /colour=7 FT /gene="ML0108" FT /note="Similar To M. tuberculosis Rv3791, putative FT oxidoreductase, SW:Y1J1_MYCTU (P72057) (254 aa); Fasta FT score E(): 0, 89.0% identity in 254 aa overlap and to FT other putative oxidoreductases from the same organism. FT Shows weaker similarity to Alcaligenes eutrophus phbB, FT acetoacetyl-CoA reductase, SW:PHBB_ALCEU (P14697) (246 FT aa); Fasta score E(): 3e-09, 28.3% identity in 191 aa FT overlap. Contains Pfam match to entry PF00106 adh_short, FT short chain dehydrogenase. Contains PS00061 Short-chain FT dehydrogenases/reductases family signature." FT /note="Shows weak similarity to ML0429 and to the FT C-terminal half of ML2565" FT /product="putative oxidoreductase" FT /tb_orthologue="Rv3791" FT misc_feature complement(149538..150098) FT /colour=0 FT /note="Pfam match to entry PF00106 adh_short, short chain FT dehydrogenase, score 83.50, E-value 4.2e-21" FT misc_feature complement(149601..149687) FT /colour=8 FT /note="PS00061 Short-chain dehydrogenases/reductases FT family signature" FT CDS complement(150128..151510) FT /class="I.B.7" FT /colour=7 FT /gene="ML0109" FT /note="Similar to M. tuberculosis Rv3790, putative FT oxidoreductase, TR:P72056 (EMBL:AL123456) (461 aa); Fasta FT score E(): 0, 86.6% identity in 461 aa overlap. Also FT similar to many bacterial putative oxidoreductases. FT Contains Pfam match to entry PF01565 FAD_binding_4, FAD FT binding domain." FT /product="putative FAD-linked oxidoreductase" FT /tb_orthologue="Rv3790" FT RBS complement(150137..150140) FT /note="possible RBS" FT misc_feature complement(150956..151489) FT /colour=0 FT /note="Pfam match to entry PF01565 FAD_binding_4, FAD FT binding domain, score -2.30, E-value 4.3e-06" FT CDS complement(151578..151949) FT /class="II.C.4" FT /colour=3 FT /gene="ML0110" FT /note="Similar to M. tuberculosis Rv3789, hypothetical FT protein, SW:Y1I9_MYCTU (P72055) (121 aa); Fasta score E(): FT 0, 73.0% identity in 122 aa overlap. Contains hydrophobic, FT possible membrane-spanning regions." FT /product="possible membrane protein" FT /tb_orthologue="Rv3789" FT RBS complement(151953..151956) FT /note="possible RBS" FT CDS complement(152204..152371) FT /class="V" FT /colour=0 FT /gene="ML0111" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3788 (Best blastx score 157)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(152783..153613) FT /class="II.C.4" FT /colour=3 FT /gene="ML0112" FT /note="Similar to M. tuberculosis Rv3783, integral FT membrane protein, TR:P72049 (EMBL:AL123456) (280 aa); FT Fasta score E(): 0, 84.3% identity in 280 aa overlap. FT Similar to Yersinia enterocolitica rfbD, O-antigen export FT system permease protein, SW:RFBD_YEREN (Z18920) (259 aa); FT Fasta score E(): 2.9e-32, 28.2% identity in 259 aa overlap FT and to other membrane proteins involved in FT lipopolysaccharide transport. Contains Pfam match to entry FT PF01061 ABC2_membrane, ABC-2 type transporter." FT /product="putative ABC transporter component" FT /tb_orthologue="Rv3783" FT misc_feature complement(152795..153556) FT /colour=0 FT /note="Pfam match to entry PF01061 ABC2_membrane, ABC-2 FT type transporter, score 276.20, E-value 4.3e-79" FT RBS complement(153624..153629) FT /note="possible RBS" FT CDS complement(153663..154514) FT /class="II.C.2" FT /colour=3 FT /gene="rfbE" FT /gene="ML0113" FT /note="Similar to M. tuberculosis Rv3782, putative FT glycosyl transferase, TR:P72048. Shows very weak FT similarity to proteins involved in lipopolysaccharide FT biosynthesis." FT /product="putative glycosyl transferase" FT /tb_orthologue="rfbE" FT CDS complement(154576..155394) FT /class="II.C.4" FT /colour=3 FT /gene="ML0114" FT /note="Similar to M. tuberculosis Rv3781, putative ABC FT transporter component, TR:P72047 (EMBL:AL123456) (273 aa); FT Fasta score E(): 0, 91.4% identity in 267 aa overlap. FT Similar to Yersinia enterocolitica rfbE, O-antigen export FT system ATP-binding protein, SW:RFBE_YEREN (Q56903) (239 FT aa); Fasta score E(): 0, 46.9% identity in 224 aa overlap FT and to other proteins involved in lipopolysaccharide FT transport. Contains Pfam match to entry PF00005 ABC_tran, FT ABC transporter. Contains PS00017 ATP/GTP-binding site FT motif A (P-loop)." FT /product="putative ABC transporter ATP-binding component" FT /tb_orthologue="Rv3781" FT misc_feature complement(154702..155214) FT /colour=0 FT /note="Pfam match to entry PF00005 ABC_tran, ABC FT transporter, score 118.80, E-value 1e-31" FT misc_feature complement(155170..155193) FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT CDS complement(155413..155937) FT /class="V" FT /colour=10 FT /gene="ML0115" FT /note="Similar to M. tuberculosis Rv3780, hypothetical FT protein, SW:Y1I0_MYCTU (P72046) (178 aa); Fasta score E(): FT 0, 82.9% identity in 170 aa overlap and to Streptomyces FT coelicolor SCGD3.23C, hypothetical protein, TR:Q9XA56 FT (EMBL:AL096822) (179 aa); Fasta score E(): 0, 65.7% FT identity in 178 aa overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv3780" FT RBS complement(155949..155954) FT /note="possible RBS" FT CDS complement(155965..157929) FT /class="II.C.4" FT /colour=3 FT /gene="ML0116" FT /note="Similar to M. tuberculosis Rv3779, unknown membrane FT protein, TR:P72045 (EMBL:AL123456) (666 aa); Fasta score FT E(): 0, 66.5% identity in 666 aa overlap. Contains FT hydrophobic, possible membrane-spanning regions." FT /product="putative membrane protein" FT /tb_orthologue="Rv3779" FT RBS 158044..158050 FT /note="possible RBS" FT CDS 158056..159252 FT /class="V" FT /colour=10 FT /gene="ML0117" FT /note="Similar to M. tuberculosis Rv3778c, conserved FT hypothetical protein, TR:P72044 (EMBL:AL123456) (398 aa); FT Fasta score E(): 0, 83.4% identity in 398 aa overlap. Also FT similar to many bacterial hypothetical proteins." FT /note="Similar to ML0596 and to the C-terminal half of FT ML0842" FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv3778c" FT CDS complement(159249..160259) FT /class="I.B.7" FT /colour=7 FT /gene="ML0118" FT /note="Similar to M. tuberculosis Rv3777, putative FT oxidireductase, TR:P72043. Similar to many oxidoreductases FT from both bacteria and higher organisms e.g. Mus musculus FT cryZ, quinone oxidoreductase, SW:QOR_MOUSE (P47199) (331 FT aa); Fasta score E(): 5.5e-21, 31.0% identity in 306 aa FT overlap. Contains Pfam match to entry PF00107 adh_zinc, FT Zinc-binding dehydrogenases." FT /note="Similar to domains of polyketide synthases ML0135, FT ML0139, ML1229 and ML2355" FT /product="putative oxidireductase" FT /tb_orthologue="Rv3777" FT misc_feature complement(159294..160232) FT /colour=0 FT /note="Pfam match to entry PF00107 adh_zinc, Zinc-binding FT dehydrogenases, score 48.50, E-value 2.4e-12" FT tRNA 160312..160401 FT /colour=4 FT /note="tRNA Ser anticodon TGA, Cove score 59.58" FT CDS complement(160495..161730) FT /class="II.B.5" FT /colour=7 FT /gene="lipE" FT /gene="ML0119" FT /note="Similar to M. tuberculosis Rv3775, probable FT hydrolase, TR:P72041 (EMBL:AL123456) (415 aa); Fasta score FT E(): 0, 84.7% identity in 406 aa overlap. Similar to FT bacterial esterases e.g. Streptomyces chrysomallus estA, FT esterase A, TR:O87861 (EMBL:Z15137) (389 aa); Fasta score FT E(): 2.6e-18, 29.9% identity in 348 aa overlap." FT /product="probable hydrolase" FT /tb_orthologue="lipE" FT RBS complement(161739..161742) FT /note="possible RBS" FT CDS complement(161741..162577) FT /class="I.A.3" FT /colour=1 FT /gene="echA1" FT /gene="ML0120" FT /note="Similar to M. tuberculosis Rv3774, putative FT enoyl-CoA hydratase, TR:P75019 (EMBL:AL123456) (274 aa); FT Fasta score E(): 0, 88.3% identity in 274 aa overlap. FT Similar to many e.g. Rattus norvegicus ech1, FT delta3,5-delta2,4-dienoyl-coa isomerase precursor, FT SW:ECH1_RAT (Q62651) (327 aa); Fasta score E(): 3.5e-31, FT 38.8% identity in 276 aa overlap and bacterial putative FT enoyl-CoA hydratases. Contains Pfam match to entry PF00378 FT ECH, Enoyl-CoA hydratase/isomerase family." FT /note="Similar to ML1241, ML1724, ML2118 and ML2402" FT /product="putative enoyl-CoA hydratase" FT /tb_orthologue="echA21" FT misc_feature complement(161975..162517) FT /colour=0 FT /note="Pfam match to entry PF00378 ECH, Enoyl-CoA FT hydratase/isomerase family, score 99.60, E-value 6.2e-26" FT CDS complement(163770..164039) FT /class="VI" FT /colour=10 FT /gene="ML0121" FT /note="Shares region of weak similarity with Streptomyces FT coelicolor SC6A5.32C, hypothetical protein, TR:Q9X7X8 FT (EMBL:AL049485) (155 aa); Fasta score E(): 0.0056, 43.1% FT identity in 58 aa overlap." FT /product="hypothetical protein" FT RBS complement(164048..164051) FT /note="possible RBS" FT CDS complement(164082..164876) FT /class="I.D.5" FT /colour=0 FT /gene="hisC2" FT /gene="ML0122" FT /note="Possible pseudogene of M. tuberculosis orthologue FT hisC2 (Best blastx score 574)" FT /product="histidinol-phosphate aminotransferase FT (pseudogene)" FT /pseudo FT tRNA 165035..165127 FT /colour=4 FT /note="tRNA Ser anticodon GCT, Cove score 56.23" FT tRNA 165147..165219 FT /colour=4 FT /note="tRNA Arg anticodon ACG, Cove score 76.52" FT CDS 166460..166833 FT /class="V" FT /colour=0 FT /gene="ML0123" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3767c (Best blastx score 169)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(166949..167389) FT /class="VI" FT /colour=10 FT /gene="ML0124" FT /note="Shows weak similarity to M. tuberculosis Rv2186c, FT hypothetical protein, TR:O53519 (EMBL:AL123456) (144 aa); FT Fasta score E(): 3.7e-06, 27.5% identity in 131 aa overlap FT and Streptomyces coelicolor SC6G10.02C, hypothetical FT protein, TR:Q9X7Y8 (EMBL:AL049497) (144 aa); Fasta score FT E(): 7e-05, 21.9% identity in 137 aa overlap. Previously FT sequenced as TR:O06090 (EMBL:Z95398) (156 aa); Fasta score FT E(): 0, 70.1% identity in 144 aa overlap." FT /note="Similar to ML2629 and shows weaker similarity to FT ML0889" FT /product="conserved hypothetical protein" FT CDS 167598..168914 FT /class="IV.H" FT /colour=7 FT /gene="ML0125" FT /note="Similar to M. tuberculosis Rv2958c, putative FT glycosyl transferase, TR:P95134 (EMBL:AL123456) (428 aa); FT Fasta score E(): 0, 74.6% identity in 418 aa overlap and FT Rv2962c, putative glycosyl transferase, TR:P95130 FT (EMBL:AL123456) (449 aa); Fasta score E(): 0, 77.5% FT identity in 426 aa overlap. Similar to Streptomyces FT venezuelae desVII, glycosyl transferase from a macrolide FT antibiotic biosynthesis gene cluster, TR:Q9ZGH7 FT (EMBL:AF079762) (426 aa); Fasta score E(): 1e-09, 30.0% FT identity in 237 aa overlap. Shows similarity to bacterial FT and eukaryotic glycosyl transferases." FT /note="Similar to ML0128 and shows weaker similarity to FT ML0985" FT /product="putative glycosyl transferase" FT /tb_orthologue="Rv2958c" FT /tb_orthologue="Rv2962c" FT RBS 169572..169578 FT /note="possible RBS" FT CDS 169585..170406 FT /class="VI" FT /colour=8 FT /gene="ML0126" FT /ml_unique FT /note="Unknown function." FT /product="hypothetical protein" FT RBS 170650..170655 FT /note="possible RBS" FT CDS 170662..171351 FT /class="VI" FT /colour=10 FT /gene="ML0127" FT /note="Similar to M. tuberculosis Rv2959c, hypothetical FT protein, TR:Q50457 (EMBL:AL123456) (245 aa); Fasta score FT E(): 0, 76.1% identity in 226 aa overlap. Shows weak FT similarity to Streptomyces clavuligerus cmcI, FT cephalosporin hydroxylase component of 7 FT alpha-cephem-methoxylase, TR:O85726 (EMBL:AF073896) (236 FT aa); Fasta score E(): 0.0001, 27.6% identity in 163 aa FT overlap and to other hypothetical proteins." FT /product="hypothetical protein" FT /tb_orthologue="Rv2959c" FT CDS 171616..172923 FT /class="IV.H" FT /colour=7 FT /gene="ML0128" FT /note="Similar to M. tuberculosis Rv2958c, putative FT glycosyl transferase, TR:P95134 (EMBL:AL123456) (428 aa); FT Fasta score E(): 0, 75.1% identity in 417 aa overlap and FT Rv2962c, putative glycosyl transferase, TR:P95130 FT (EMBL:AL123456) (449 aa); Fasta score E(): 0, 74.4% FT identity in 425 aa overlap. Similar to Streptomyces FT venezuelae desVII, glycosyl transferase from a macrolide FT antibiotic biosynthesis gene cluster, TR:Q9ZGH7 FT (EMBL:AF079762) (426 aa); Fasta score E(): 1.1e-09, 30.9% FT identity in 223 aa overlap. Shows similarity to bacterial FT and eukaryotic glycosyl transferases." FT /note="Similar to ML0125 and shows weaker similarity to FT ML0985" FT /product="putative glycosyl transferase" FT /tb_orthologue="Rv2958c" FT /tb_orthologue="Rv2962c" FT CDS complement(173170..174426) FT /class="V" FT /colour=10 FT /gene="ML0129" FT /note="Similar to M. tuberculosis Rv2953, conserved FT hypothetical protein, TR:P95139 (EMBL:AL123456) (418 aa); FT Fasta score E(): 0, 86.6% identity in 418 aa overlap and FT to Rv2449c, conserved hypothetical protein, TR:O53176 FT (EMBL:AL123456) (419 aa); Fasta score E(): 0, 64.9% FT identity in 419 aa overlap. Similar to many bacterial FT hypothetical proteins." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv2449c" FT CDS complement(174466..175278) FT /class="V" FT /colour=10 FT /gene="ML0130" FT /note="Similar to M.tuberculosis Rv2952, hypothetical FT protein, TR:Q50464 (EMBL:AL123456) (270 aa); Fasta score FT E(): 0, 83.7% identity in 270 aa overlap. Similar to in FT part to Streptomyces hygroscopicus rapM, methyltransferase FT involved in rapamycin biosynthesis, TR:Q54303 FT (EMBL:X86780) (317 aa); Fasta score E(): 5e-14, 38.6% FT identity in 158 aa overlap and shows weak similarity to FT CDS associated with several gene clusters encoding FT polyketide biosynthesis." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv2952" FT CDS 175441..176589 FT /class="I.B.7" FT /colour=7 FT /gene="ML0131" FT /note="Similar to M. tuberculosis Rv2951c, putative FT oxidoreductase, TR:P95140 (EMBL:AL123456) (381 aa); Fasta FT score E(): 0, 84.8% identity in 382 aa overlap. Similar to FT Archaeoglobus fulgidus AF2411, conserved hypothetical FT protein, TR:O30260 (EMBL:AE001109) (363 aa); Fasta score FT E(): 0, 31.5% identity in 362 aa overlap. Shows weak FT similarity to bacterial oxidoreductases e.g. Mycobacterium FT smegmatis fgd, F420-dependent glucose-6-phosphate FT dehydrogenase, TR:O68447 (EMBL:AF041061) (336 aa); Fasta FT score E(): 1.1e-09, 27.7% identity in 292 aa overlap." FT /note="Shows weak similarity to ML0269" FT /product="putative oxidoreductase" FT /tb_orthologue="Rv2951c" FT CDS 177170..179212 FT /class="I.A.3" FT /colour=1 FT /gene="fadD29" FT /gene="ML0132" FT /note="Similar to several putative acyl-CoA synthases from FT M. tuberculosis e.g. M. tuberculosis Rv2950c, fadD29, FT putative acyl-CoA synthase, TR:P95141 (EMBL:AL123456) (619 FT aa); Fasta score E(): 0, 80.2% identity in 620 aa overlap. FT Similar to Mycobacterium bovis BCG ORF3, acyl-CoA FT synthase, TR:P71495 (EMBL:U75685) (582 aa); Fasta score FT E(): 0, 58.5% identity in 585 aa overlap and to domains of FT polyketide synthases e.g. Myxococcus xanthus safB, FT saframycin Mx1 synthetase B, TR:Q50857 (EMBL:U24657) (1770 FT aa); Fasta score E(): 0, 36.2% identity in 589 aa overlap. FT Contains Pfam match to entry PF00501 AMP-binding, FT AMP-binding enzyme." FT /note="Similar to ML0100, ML0138, ML1234 and ML2358" FT /product="putative acyl-CoA synthetase" FT /tb_orthologue="fadD29" FT misc_feature 177581..178942 FT /colour=0 FT /note="Pfam match to entry PF00501 AMP-binding, FT AMP-binding enzyme, score -14.90, E-value 1.4e-14" FT CDS 179256..179888 FT /class="VI" FT /colour=10 FT /gene="ML0133" FT /note="Similar to M. tuberculosis Rv2949c, hypothetical FT protein, TR:O86325 (EMBL:AL123456) (199 aa); Fasta score FT E(): 0, 62.6% identity in 195 aa overlap. Shows weaker FT similarity to Archaeoglobus fulgidus AF1396, hypothetical FT protein, TR:O28875 (EMBL:AE001008) (162 aa); Fasta score FT E(): 4.2e-05, 26.6% identity in 154 aa overlap. Contains FT Pfam match to entry PF01947 DUF98, Protein of unknown FT function." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv2949c" FT misc_feature 179295..179786 FT /colour=0 FT /note="Pfam match to entry PF01947 DUF98, Protein of FT unknown function, score -37.20, E-value 0.0013" FT CDS 180027..182150 FT /class="I.A.3" FT /colour=1 FT /gene="fadD22" FT /gene="ML0134" FT /note="Similar to M. tuberculosis Rv2948c, acyl-CoA FT synthase, TR:P96283 (EMBL:AL123456) (705 aa); Fasta score FT E(): 0, 75.9% identity in 705 aa overlap. Similar to many FT acyl-CoA synthases e.g. Rhodopseudomonas palustris, badA, FT benzoate-coenzyme A ligase, TR:Q59760 (EMBL:L42322) (521 FT aa); Fasta score E(): 0, 31.4% identity in 494 aa overlap. FT Contains Pfam match to entry PF00550 pp-binding, FT Phosphopantetheine attachment site. Contains Pfam match to FT entry PF00501 AMP-binding, AMP-binding enzyme." FT /note="Similar to ML1051, ML1994, ML2257 and ML2546" FT /product="putative acyl-CoA synthetase" FT /tb_orthologue="fadD22" FT misc_feature 180120..181280 FT /colour=0 FT /note="Pfam match to entry PF00501 AMP-binding, FT AMP-binding enzyme, score 66.50, E-value 4e-19" FT misc_feature 181674..181877 FT /colour=0 FT /note="Pfam match to entry PF00550 pp-binding, FT Phosphopantetheine attachment site, score 43.60, E-value FT 2e-10" FT RBS 182156..182160 FT /note="possible RBS" FT CDS 182173..188484 FT /class="I.I" FT /colour=1 FT /gene="ML0135" FT /note="Similar to many polyketide synthases from M. FT tuberculosis e.g. N-terminus is similar to Rv2947c, pks15, FT probable polyketide synthase, TR:P96284 (EMBL:Z83858) (496 FT aa); Fasta score E(): 0, 90.5% identity in 485 aa overlap FT and the remainder is similar to Rv2946c, pks1, probable FT polyketide synthase, TR:P96285 (EMBL:Z83858) (1616 aa); FT Fasta score E(): 0, 82.5% identity in 1620 aa overlap. FT Similar to many others e.g. Streptomyces noursei NysC, FT nystatin biosynthesis polyketide synthase, TR:AAF71776 FT (EMBL:AF263912) (11096 aa); Fasta score E(): 0, 50.2% FT identity in 2161 aa overlap. Contains Pfam match to entry FT PF00107 adh_zinc, Zinc-binding dehydrogenases. Contains FT Pfam match to entry PF00109 ketoacyl-synt, Beta-ketoacyl FT synthase. Contains Pfam match to entry PF00698 FT Acyl_transf, Acyl transferase domain. Contains Pfam match FT to entry PF00550 pp-binding, Phosphopantetheine attachment FT site. Contains PS00012 Phosphopantetheine attachment site. FT Contains PS00606 Beta-ketoacyl synthases active site." FT /note="Similar to ML0101, ML0139, ML1229, ML2353, ML2354, FT ML2355, ML2356 and ML2357" FT /product="putative polyketide synthase" FT /tb_orthologue="pks1" FT /tb_orthologue="pks15" FT misc_feature 182302..183570 FT /colour=0 FT /note="Pfam match to entry PF00109 ketoacyl-synt, FT Beta-ketoacyl synthase, score 753.90, E-value 6.6e-223" FT misc_feature 182776..182826 FT /colour=8 FT /note="PS00606 Beta-ketoacyl synthases active site" FT misc_feature 183877..184821 FT /colour=0 FT /note="Pfam match to entry PF00698 Acyl_transf, Acyl FT transferase domain, score 226.70, E-value 3.4e-64" FT misc_feature 186370..187281 FT /colour=0 FT /note="Pfam match to entry PF00107 adh_zinc, Zinc-binding FT dehydrogenases, score -67.70, E-value 3.9e-06" FT misc_feature 188185..188388 FT /colour=0 FT /note="Pfam match to entry PF00550 pp-binding, FT Phosphopantetheine attachment site, score 76.30, E-value FT 1.7e-19" FT misc_feature 188263..188310 FT /colour=8 FT /note="PS00012 Phosphopantetheine attachment site" FT RBS 188537..188540 FT /note="possible RBS" FT CDS 188551..189252 FT /class="II.C.1" FT /colour=3 FT /gene="lppX" FT /gene="ML0136" FT /note="Similar to M. tuberculosis lipoproteins e.g. M. FT tuberculosis lppX, putative lipoprotein, SW:LPPX_MYCTU FT (Z83858) (233 aa); Fasta score E(): 0, 76.4% identity in FT 233 aa overlap. Similar to Mycobacterium bovis lprG, 27 FT kDa lipoprotein antigen, SW:LPRG_MYCTU (AJ000500) (236 FT aa); Fasta score E(): 2.5e-13, 31.2% identity in 202 aa FT overlap. Contains a probable N-terminal signal sequence. FT Contains PS00013 Prokaryotic membrane lipoprotein lipid FT attachment site." FT /note="Similar to ML0557" FT /product="putative lipoprotein" FT /tb_orthologue="lppX" FT misc_feature 188575..188607 FT /colour=8 FT /note="PS00013 Prokaryotic membrane lipoprotein lipid FT attachment site" FT CDS complement(189253..191961) FT /class="II.C.4" FT /colour=3 FT /gene="mmpL7" FT /gene="ML0137" FT /note="Similar to members of the M. tuberculosis mmpL FT family of membrane proteins e.g. Rv2942, mmpL7, putative FT membrane protein, SW:MML7_MYCTU (P96289) (920 aa); Fasta FT score E(): 0, 68.6% identity in 881 aa overlap. Contains FT hydrophobic, possible membrane-spanning regions." FT /note="Similar to ML1231 and ML2378. Similar to pseudogene FT ML2375" FT /product="putative membrane protein" FT /tb_orthologue="mmpL7" FT CDS complement(192013..193752) FT /class="I.A.3" FT /colour=1 FT /gene="fadD28" FT /gene="ML0138" FT /note="Similar to several putative acyl-CoA synthases from FT M. tuberculosis e.g. M. tuberculosis Rv2941, fadD29, FT putative acyl-CoA synthase, TR:P96290 (EMBL:AL123456) (580 FT aa); Fasta score E(): 0, 81.9% identity in 580 aa overlap. FT Similar to Mycobacterium bovis BCG ORF3, acyl-CoA FT synthase, TR:P71495 (EMBL:U75685) (582 aa); Fasta score FT E(): 0, 81.4% identity in 580 aa overlap and to domains of FT polyketide synthases e.g. Myxococcus xanthus safB, FT saframycin Mx1 synthetase B, TR:Q50857 (EMBL:U24657) (1770 FT aa); Fasta score E(): 0, 35.8% identity in 590 aa overlap. FT Contains Pfam match to entry PF00501 AMP-binding, FT AMP-binding enzyme." FT /note="Similar to ML0100, ML0132, ML0243, ML1234, ML2358" FT /product="acyl-CoA synthetase" FT /tb_orthologue="fadD28" FT misc_feature complement(192277..193635) FT /colour=0 FT /note="Pfam match to entry PF00501 AMP-binding, FT AMP-binding enzyme, score -8.20, E-value 5.9e-15" FT RBS 194207..194210 FT /note="possible RBS" FT CDS 194221..200571 FT /class="I.I" FT /colour=1 FT /gene="mas" FT /gene="ML0139" FT /note="Similar to many polyketide synthases from M. FT tuberculosis e.g. Rv2940c, mas, mycocerosic acid synthase, FT TR:P96291 (EMBL:AL123456) (2111 aa); Fasta score E(): 0, FT 88.0% identity in 2119 aa overlap. Similar to FT Mycobacterium bovis mas, mycocerosic acid synthase, FT SW:MCAS_MYCBO (Q02251) (2110 aa); Fasta score E(): 0, FT 85.1% identity in 2122 aa overlap. Contains Pfam match to FT entry PF00107 adh_zinc, Zinc-binding dehydrogenases. FT Contains Pfam match to entry PF00109 ketoacyl-synt, FT Beta-ketoacyl synthase. Contains Pfam match to entry FT PF00698 Acyl_transf, Acyl transferase domain. Contains FT Pfam match to entry PF00550 pp-binding, Phosphopantetheine FT attachment site. Contains PS00012 Phosphopantetheine FT attachment site. Contains PS00606 Beta-ketoacyl synthases FT active site." FT /note="Similar to ML0101, ML0135, ML1229, ML2353, ML2354, FT ML2355, ML2356 and ML2357" FT /product="putative mycocerosic synthase" FT /tb_orthologue="mas" FT misc_feature 194242..195510 FT /colour=0 FT /note="Pfam match to entry PF00109 ketoacyl-synt, FT Beta-ketoacyl synthase, score 619.80, E-value 1.6e-182" FT misc_feature 194722..194772 FT /colour=8 FT /note="PS00606 Beta-ketoacyl synthases active site" FT misc_feature 195832..196791 FT /colour=0 FT /note="Pfam match to entry PF00698 Acyl_transf, Acyl FT transferase domain, score 500.60, E-value 1.2e-146" FT misc_feature 198499..199440 FT /colour=0 FT /note="Pfam match to entry PF00107 adh_zinc, Zinc-binding FT dehydrogenases, score -26.80, E-value 2.5e-08" FT misc_feature 200299..200502 FT /colour=0 FT /note="Pfam match to entry PF00550 pp-binding, FT Phosphopantetheine attachment site, score 71.50, E-value FT 3.6e-18" FT misc_feature 200377..200424 FT /colour=8 FT /note="PS00012 Phosphopantetheine attachment site" FT CDS 200930..201848 FT /class="V" FT /colour=0 FT /gene="ML0140" FT /note="Possible pseudogene similar to M. tuberculosis FT paralogue Rv0221 (Best blastx score 157)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(202307..202561) FT /class="VI" FT /colour=8 FT /gene="ML0141" FT /ml_unique FT /note="Improbable CDS suggested by GC frameplot. Possibly FT the remains of a disrupted gene." FT /product="hypothetical protein" FT CDS 204363..204611 FT /class="II.C.5" FT /colour=3 FT /gene="ML0142" FT /note="Unknown function. Contains hydrophobic, possible FT membrane-spanning region." FT /product="possible membrane protein" FT repeat_region complement(204534..207280) FT CDS complement(204890..204988) FT /class="IV.B.1.c" FT /colour=0 FT /gene="ML0143" FT /note="Similar to Agrobacterium tumefaciens transposase FT TR:Q44454 (EMBL:Z18270) (366 aa) fasta scores: E(): FT 0.0002, 51.5% id in 33 aa, and to Pseudomonas putida FT transposase tnpa1 TR:Q9R9U9 (EMBL:AJ245436) (355 aa) fasta FT scores: E(): 0.00088, 48.5% id in 33 aa" FT /pseudo FT CDS complement(205253..206396) FT /class="IV.B" FT /colour=0 FT /gene="ML0144" FT /note="Similar to many e.g. Cryphonectria parasitica FT (Chesnut blight fungus) putative maturase TR:AAF27656 FT (EMBL:AF218567) (778 aa) fasta scores: E(): 7.8e-11, 29.2% FT id in 216 aa" FT /product="putative group II intron maturase-related FT protein" FT /pseudo FT stem_loop complement(205716..205769) FT CDS complement(206601..206740) FT /class="IV.B.1.c" FT /colour=0 FT /gene="ML0145" FT /note="Similar to Pseudomonas putida transposase tnpa1 FT TR:Q9R9U9 (EMBL:AJ245436) (355 aa) fasta scores: E(): FT 3.4e-05, 47.8% id in 46 aa, and to Agrobacterium FT tumefaciens transposase TR:Q44454 (EMBL:Z18270) (366 aa) FT fasta scores: E(): 0.00026, 41.3% id in 46 aa" FT /pseudo FT /product="transposase (pseudogene)" FT CDS complement(207844..207954) FT /class="V" FT /colour=0 FT /gene="ML0146" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv0941c (Best blastx score 108)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT misc_feature 208911..209192 FT /colour=0 FT /note="Pfam match to entry PF00934 PE, PE family, score FT 116.70, E-value 4.4e-31" FT CDS 208911..209279 FT /note="Similar to the N-terminal, non-repetitive domains FT of M. tuberculosis PE-family proteins e.g. Rv1803c, FT PE_family, PGRS-subfamily protein, TR:O53952. The start FT codon appears to have been mutated (ATG to ATA)" FT /class="IV.C.1" FT /colour=0 FT /fasta_match="TR:O53952 (EMBL:AL123456)" FT /gene="ML0147" FT /pseudo FT CDS 209823..210314 FT /class="II.A.5" FT /colour=0 FT /gene="ML0148" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv0944 (Best blastx score 261)" FT /product="possible formamidopyrimidine-DNA glycosylase FT (pseudogene)" FT /pseudo FT CDS 210338..211058 FT /class="I.B.7" FT /colour=0 FT /gene="ML0149" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv0945 (Best blastx score 196)" FT /product="ketoacyl reductase (pseudogene)" FT /pseudo FT CDS complement(211150..212814) FT /EC_number="5.3.1.9" FT /class="I.B.1" FT /colour=7 FT /gene="pgi" FT /gene="ML0150" FT /note="Similar to M. tuberculosis Rv0946c, pgi, FT glucose-6-phosphate isomerase, SW:G6PI_MYCTU (P77895) (553 FT aa); Fasta score E(): 0, 85.5% identity in 552 aa overlap FT and Mycobacterium smegmatis pgi, glucose-6-phosphate FT isomerase, SW:G6PI_MYCSM (P96803) (442 aa); Fasta score FT E(): 0, 84.1% identity in 440 aa overlap. Contains Pfam FT match to entry PF00342 PGI, Phosphoglucose isomerase. FT Contains PS00765 Phosphoglucose isomerase signature 1. FT Contains PS00174 Phosphoglucose isomerase signature 2." FT /product="glucose-6-phosphate isomerase" FT /tb_orthologue="pgi" FT misc_feature complement(211186..212652) FT /colour=0 FT /note="Pfam match to entry PF00342 PGI, Phosphoglucose FT isomerase, score 794.70, E-value 3.5e-235" FT misc_feature complement(211270..211323) FT /colour=8 FT /note="PS00174 Phosphoglucose isomerase signature 2" FT misc_feature complement(211972..212013) FT /colour=8 FT /note="PS00765 Phosphoglucose isomerase signature 1" FT CDS complement(213175..213492) FT /class="V" FT /colour=10 FT /gene="ML0151" FT /note="Similar to M. tuberculosis Rv0948c, hypothetical FT protein, SW:Y948_MYCTU (P71562) (105 aa); Fasta score E(): FT 1.9e-32, 83.8% identity in 105 aa overlap. Also similar to FT Streptomyces coelicolor SCD63.16C, hypothetical protein, FT TR:CAB82023 (EMBL:AL161755) (110 aa); Fasta score E(): FT 3.9e-06, 44.4% identity in 72 aa overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv0948c" FT RBS complement(213504..213507) FT /note="possible RBS" FT CDS 214001..214189 FT /class="VI" FT /colour=8 FT /gene="ML0152" FT /ml_unique FT /note="Improbable CDS suggested by GC frameplot. Possibly FT the remains of a disrupted gene." FT /product="hypothetical protein" FT CDS 214272..216608 FT /class="II.A.5" FT /colour=2 FT /gene="uvrD" FT /gene="ML0153" FT /note="Similar to M. tuberculosis Rv0949, uvrD, putative FT DNA-dependent ATPase I and helicase II, TR:P71561 FT (EMBL:AL123456) (771 aa); Fasta score E(): 0, 81.4% FT identity in 780 aa overlap. Similar to many DNA helicases FT e.g. Bacillus stearothermophilus pcrA, ATP-dependent FT helicase, SW:PCRA_BACST (P56255) (724 aa); Fasta score FT E(): 0, 43.8% identity in 774 aa overlap. Contains Pfam FT match to entry PF00580 UvrD-helicase, UvrD/REP helicase. FT Contains PS00017 ATP/GTP-binding site motif A (P-loop)." FT /note="Similar to ML0637" FT /product="putative ATP-dependent DNA helicase" FT /tb_orthologue="uvrD" FT misc_feature 214335..215804 FT /colour=0 FT /note="Pfam match to entry PF00580 UvrD-helicase, UvrD/REP FT helicase, score 416.10, E-value 3.4e-121" FT misc_feature 214389..214412 FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT CDS complement(217160..218161) FT /class="V" FT /colour=10 FT /gene="ML0154" FT /note="Similar to M. tuberculosis Rv0950c, hypothetical FT protein, TR:P71560 (EMBL:AL123456) (332 aa); Fasta score FT E(): 0, 71.0% identity in 331 aa overlap. Shows weaker FT similarity to many bacterial hypothetical proteins which FT contains a Peptidase family M23/M37 domain. Previously FT sequenced as TR:Q9Z5H9 (EMBL:AL035500) (343 aa); Fasta FT score E(): 0, 100.0% identity in 333 aa overlap. Contains FT Pfam match to entry PF01551 Peptidase_M37, Peptidase FT family M23/M37." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv0950c" FT misc_feature complement(217202..217453) FT /colour=0 FT /note="Pfam match to entry PF01551 Peptidase_M37, FT Peptidase family M23/M37, score 144.20, E-value 2.2e-39" FT CDS 218458..219639 FT /EC_number="6.2.1.5" FT /class="I.B.3" FT /colour=7 FT /gene="sucC" FT /gene="ML0155" FT /note="Similar to M. tuberculosis Rv0951, sucC, putative FT succinyl-CoA synthase [beta] chain, SW:SUCC_MYCTU (P71559) FT (387 aa); Fasta score E(): 0, 86.7% identity in 391 aa FT overlap. Similar to many e.g. Thermus aquaticus sucC, FT succinyl-CoA synthase [beta] chain, SW:SUCC_THEFL (P25126) FT (378 aa); Fasta score E(): 0, 48.7% identity in 384 aa FT overlap. Previously sequenced as TR:Q9Z5H8 (EMBL:AL035500) FT (393 aa); Fasta score E(): 0, 100.0% identity in 393 aa FT overlap. Contains Pfam match to entry PF00549 ligase-CoA, FT CoA-ligases. Contains Pfam match to entry PF02222 FT ATP-grasp, ATP-grasp domain. Contains PS01217 ATP-citrate FT lyase / succinyl-CoA ligases family signature 3." FT /product="succinyl-CoA synthase [beta] chain" FT /tb_orthologue="sucC" FT misc_feature 218542..219027 FT /colour=0 FT /note="Pfam match to entry PF02222 ATP-grasp, ATP-grasp FT domain, score 164.40, E-value 1.9e-45" FT misc_feature 219187..219564 FT /colour=0 FT /note="Pfam match to entry PF00549 ligase-CoA, FT CoA-ligases, score 129.00, E-value 6.8e-35" FT misc_feature 219220..219294 FT /colour=8 FT /note="PS01217 ATP-citrate lyase / succinyl-CoA ligases FT family signature 3" FT RBS 219639..219644 FT /note="possible RBS" FT CDS 219659..220561 FT /EC_number="6.2.1.5" FT /class="I.B.3" FT /colour=7 FT /gene="sucD" FT /gene="ML0156" FT /note="Similar to M. tuberculosis Rv0952, sucD, putative FT succinyl-CoA synthase [alpha] chain, SW:SUCD_MYCTU FT (P71558) (303 aa); Fasta score E(): 0, 86.3% identity in FT 300 aa overlap. Similar to many e.g. Escherichia coli FT sucD, succinyl-CoA synthase [alpha] chain, SW:SUCD_ECOLI FT (P07459) (288 aa); Fasta score E(): 0, 50.3% identity in FT 298 aa overlap. Previously sequenced as TR:Q9S372 FT (EMBL:AL035500) (300 aa); Fasta score E(): 0, 100.0% FT identity in 300 aa overlap. Contains Pfam match to entry FT PF00549 ligase-CoA, CoA-ligases. Contains PS00017 FT ATP/GTP-binding site motif A (P-loop). Contains PS00399 FT ATP-citrate lyase / succinyl-CoA ligases family active FT site. Contains PS01216 ATP-citrate lyase / succinyl-CoA FT ligases family signature 1." FT /product="succinyl-CoA synthase [alpha] chain" FT /tb_orthologue="sucD" FT misc_feature 220088..220525 FT /colour=0 FT /note="Pfam match to entry PF00549 ligase-CoA, FT CoA-ligases, score 115.90, E-value 3.2e-31" FT misc_feature 220145..220228 FT /colour=8 FT /note="PS01216 ATP-citrate lyase / succinyl-CoA ligases FT family signature 1" FT misc_feature 220391..220432 FT /colour=8 FT /note="PS00399 ATP-citrate lyase / succinyl-CoA ligases FT family active site" FT misc_feature 220490..220513 FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT CDS complement(220591..221452) FT /class="I.B.7" FT /colour=0 FT /gene="ML0157" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv0953c (Best blastx score 518)" FT /product="similar to alkanal monooxygenase beta chains FT (pseudogene)" FT /pseudo FT CDS 221657..222601 FT /class="II.C.2" FT /colour=3 FT /gene="ML0158" FT /note="Similar to M. tuberculosis Rv0954, 34 kDa antigenic FT protein homologue, SW:34KD_MYCTU (P71556) (303 aa); Fasta FT score E(): 0, 55.8% identity in 312 aa overlap and to FT Mycobacterium paratuberculosis 34 kDa antigenic protein, FT SW:34KD_MYCPA (Q04959) (298 aa); Fasta score E(): 0, 61.7% FT identity in 311 aa overlap. Previously sequenced as FT TR:O87894 (EMBL:U82111) (336 aa); Fasta score E(): 0, FT 99.7% identity in 314 aa overlap. Contains hydrophobic, FT membrane-spanning regions." FT /product=" 34 kDa antigen" FT RBS 222637..222640 FT /note="possible RBS" FT CDS 222650..223942 FT /class="II.C.4" FT /colour=3 FT /gene="ML0159" FT /note="Similar to M. tuberculosis Rv0955, possible FT membrane protein, SW:Y955_MYCTU (P71555) (455 aa); Fasta FT score E(): 0, 75.9% identity in 419 aa overlap. Previously FT sequenced as TR:Q9Z5H7 (EMBL:AL035500) (430 aa); Fasta FT score E(): 0, 99.8% identity in 430 aa overlap. Contains FT hydrophobic, possible membrane-spanning regions." FT /product="putative membrane protein" FT /tb_orthologue="Rv0955" FT CDS 223972..224619 FT /EC_number="2.1.2.2" FT /class="I.F.1" FT /colour=7 FT /gene="purN" FT /gene="ML0160" FT /note="Similar to M. tuberculosis Rv0956, purN, putative FT phosphoribosylglycinamide formyltransferase, TR:P71554 FT (EMBL:AL123456) (215 aa); Fasta score E(): 0, 79.4% FT identity in 214 aa overlap. Similar to many e.g. FT Mycobacterium avium subsp. paratuberculosis purN, FT phosphoribosylglycinamide formyltransferase I, TR:Q9RAJ6 FT (EMBL:AF191543) (209 aa); Fasta score E(): 0, 81.6% FT identity in 207 aa overlap. Previously sequenced as FT TR:Q9Z5H6 (EMBL:AL035500) (215 aa); Fasta score E(): 0, FT 99.5% identity in 215 aa overlap. Contains Pfam match to FT entry PF00551 formyl_transf, Formyl transferase." FT /product="putative phosphoribosylglycinamide FT formyltransferase" FT /tb_orthologue="purN" FT misc_feature 224008..224544 FT /colour=0 FT /note="Pfam match to entry PF00551 formyl_transf, Formyl FT transferase, score 156.30, E-value 6.8e-44" FT CDS 224616..226199 FT /EC_number="2.1.2.3" FT /EC_number="3.5.4.10" FT /class="I.F.1" FT /colour=7 FT /gene="purH" FT /gene="ML0161" FT /note="Similar to M. tuberculosis Rv0957, purH, putative FT phosphoribosylaminoimidazolecarboxamide formyltransferase FT / IMP cyclohydrolase, SW:PUR9_MYCTU (P71553) (523 aa); FT Fasta score E(): 0, 88.1% identity in 520 aa overlap. FT Similar to many e.g. Bacillus subtilis purN, FT phosphoribosylaminoimidazolecarboxamide formyltransferase FT / IMP cyclohydrolase, SW:PUR9_BACSU (P12048) (512 aa); FT Fasta score E(): 0, 43.2% identity in 519 aa overlap. FT Previously sequenced as SW:PUR9_MYCLE (Q9Z5H5) (527 aa); FT Fasta score E(): 0, 100.0% identity in 527 aa overlap. FT Contains Pfam match to entry PF01808 AICARFT_IMPCHas, FT AICARFT/IMPCHase bienzyme." FT /product="putative phosphoribosylaminoimidazolecarboxamide FT formyltransferase / IMP cyclohydrolase" FT /tb_orthologue="purH" FT misc_feature 225048..225992 FT /colour=0 FT /note="Pfam match to entry PF01808 AICARFT_IMPCHas, FT AICARFT/IMPCHase bienzyme, score 585.50, E-value 3.2e-172" FT CDS complement(227008..227286) FT /class="VI" FT /colour=8 FT /gene="ML0162" FT /ml_unique FT /note="Improbable CDS suggested by GC frameplot. Possibly FT the remains of a disrupted gene. Previously sequenced as FT TR:Q9Z5H3 (EMBL:AL035500) (92 aa); Fasta score E(): 0, FT 98.9% identity in 92 aa overlap." FT /product="hypothetical protein" FT CDS 228661..229628 FT /class="V" FT /colour=0 FT /gene="ML0163" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv0959 (Best blastx score 266)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS 229629..230493 FT /class="I.A.3" FT /colour=0 FT /gene="fadE13" FT /gene="ML0164" FT /note="Possible pseudogene of M. tuberculosis orthologue FT fadE13 (Best blastx score 280)" FT /product="acyl-CoA dehydrogenase (pseudogene)" FT /pseudo FT CDS 230546..231663 FT /class="I.A.3" FT /colour=0 FT /gene="accD2" FT /gene="ML0165" FT /note="Possible pseudogene of M. tuberculosis orthologue FT accD2 (Best blastx score 451)" FT /product="acetyl/propionyl-CoA carboxylase, [beta] subunit FT (pseudogene)" FT /pseudo FT CDS 231672..233398 FT /class="I.A.3" FT /colour=0 FT /gene="accA2" FT /gene="ML0166" FT /note="Possible pseudogene of M. tuberculosis orthologue FT accA2 (Best blastx score 317)" FT /product="acetyl/propionyl-CoA carboxylase, [alpha] FT subunit (pseudogene)" FT /pseudo FT CDS 233449..234077 FT /class="I.A.3" FT /colour=0 FT /gene="fadE12" FT /gene="ML0167" FT /note="Possible pseudogene of M. tuberculosis orthologue FT fadE12 (Best blastx score 396)" FT /product="acyl-CoA dehydrogenase (pseudogene)" FT /pseudo FT CDS 234124..234820 FT /class="I.A.3" FT /colour=0 FT /gene="echA7" FT /gene="ML0168" FT /note="Possible pseudogene of M. tuberculosis orthologue FT echA7 (Best blastx score 174)" FT /product="enoyl-CoA hydratase/isomerase superfamily FT (pseudogene)" FT /pseudo FT CDS 234932..235534 FT /class="V" FT /colour=10 FT /gene="ML0169" FT /note="Similar to M. tuberculosis Rv0966c, hypothetical FT protein, SW:Y966_MYCTU (P71544) (230 aa); Fasta score E(): FT 0, 79.5% identity in 200 aa overlap. Also similar to FT Streptomyces coelicolor SCE6.30C, hypothetical protein, FT TR:CAB88834 (EMBL:AL353832) (277 aa); Fasta score E(): FT 3.3e-20, 41.0% identity in 205 aa overlap. Previously FT sequenced as TR:Q9Z5H1 (EMBL:AL035500) (200 aa); Fasta FT score E(): 0, 100.0% identity in 200 aa overlap." FT /product="conserved hypothetical protein" FT CDS 235667..235822 FT /class="V" FT /colour=0 FT /gene="ML0170" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv0965c (Best blastx score 115)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS 236614..237352 FT /class="V" FT /colour=0 FT /gene="ML0171" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv0963c (Best blastx score 311)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS 237609..238202 FT /class="V" FT /colour=0 FT /gene="ML0172" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv0962c (Best blastx score 310)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT repeat_region 238251..239837 FT CDS complement(240228..240347) FT /class="V" FT /colour=0 FT /gene="ML0173" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv0979c (Best blastx score 105)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT misc_feature 240531..240866 FT /colour=0 FT /note="Pfam match to entry PF00072 response_reg, Response FT regulator receiver domain, score 158.60, E-value 1.1e-43" FT CDS 240531..241217 FT /class="I.J.2" FT /colour=9 FT /gene="ML0174" FT /note="Similar to several putative two-component response FT regulators from M. tuberculosis e.g. Rv0981, putative FT two-component response regulator, TR:O53894 FT (EMBL:AL123456) (230 aa); Fasta score E(): 0, 94.3% FT identity in 228 aa overlap. Similar to many others e.g. FT Pseudomonas syringae copR, transcriptional activator FT protein involved in activation of copper resistance, FT SW:COPR_PSESM (Q02540) (227 aa); Fasta score E(): 6.4e-32, FT 43.9% identity in 223 aa overlap. Previously sequenced as FT TR:Q9Z5G8 (EMBL:AL035500) (253 aa); Fasta score E(): 0, FT 100.0% identity in 228 aa overlap. Contains Pfam match to FT entry PF00486 trans_reg_C, Transcriptional regulatory FT protein, C terminal. Contains Pfam match to entry PF00072 FT response_reg, Response regulator receiver domain." FT /note="Similar to ML0773, ML1286, ML2123 and ML2439" FT /product="putative two-component response regulator" FT /tb_orthologue="Rv0981" FT misc_feature 240975..241190 FT /colour=0 FT /note="Pfam match to entry PF00486 trans_reg_C, FT Transcriptional regulatory protein, C terminal, score FT 85.90, E-value 2.2e-24" FT RBS 241199..241205 FT /note="possible RBS" FT CDS 241217..242776 FT /class="I.J.2" FT /colour=9 FT /gene="ML0175" FT /note="Similar to several putative two-component system FT sensor kinases from M. tuberculosis e.g. Rv0982, putative FT two-component system sensor kinase, TR:O53895 FT (EMBL:AL123456) (504 aa); Fasta score E(): 0, 81.0% FT identity in 521 aa overlap. Similar to many others e.g. FT Pseudomonas syringae copS, sensor protein involved in FT activation of copper resistance, SW:COPS_PSESM (Q02541) FT (487 aa); Fasta score E(): 1.2e-18, 27.9% identity in 427 FT aa overlap. Previously sequenced as TR:Q9Z5G7 FT (EMBL:AL035500) (519 aa); Fasta score E(): 0, 100.0% FT identity in 519 aa overlap. Contains hydrophobic, FT possible membrane-spanning regions. Contains Pfam match to FT entry PF00672 DUF5, Domain found in bacterial signal FT proteins. Contains Pfam match to entry PF00512 signal, FT Histidine kinase. Contains PS00017 ATP/GTP-binding site FT motif A (P-loop)." FT /note="Similar to ML0774, ML2124 and ML2440" FT /product="putative two-component system sensor kinase" FT /tb_orthologue="Rv0982" FT misc_feature 241712..241921 FT /colour=0 FT /note="Pfam match to entry PF00672 DUF5, Domain found in FT bacterial signal proteins, score 65.20, E-value 1.4e-15" FT misc_feature 241961..242605 FT /colour=0 FT /note="Pfam match to entry PF00512 signal, Histidine FT kinase, score 137.60, E-value 2.2e-37" FT misc_feature 242690..242713 FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT CDS 243054..244202 FT /class="II.B.3" FT /colour=7 FT /gene="ML0176" FT /note="Similar to M.tuberculosis Rv0983, probable serine FT protease, TR:O53896 (EMBL:AL123456) (464 aa); Fasta score FT E(): 0, 80.7% identity in 383 aa overlap and in part to FT M.tuberculosis htrA, putative serine protease, TR:O06291 FT (EMBL:AL123456) (549 aa); Fasta score E(): 0, 41.8% FT identity in 347 aa overlap. Similar to many e.g. putative FT serine protease expressed in vivo by Mycobacterium avium FT subsp. paratuberculosis, TR:Q50320 (EMBL:Z23092) (361 aa); FT Fasta score E(): 1.1e-19, 35.4% identity in 362 aa FT overlap. Previously sequenced as TR:Q9Z5G6 (EMBL:AL035500) FT (452 aa); Fasta score E(): 0, 100.0% identity in 382 aa FT overlap. Contains a possible N-terminal signal sequence. FT Contains Pfam match to entry PF00595 PDZ, PDZ domain (Also FT known as DHR or GLGF).. Contains Pfam match to entry FT PF00089 trypsin, Trypsin." FT /note="Similar to ML1078 and ML2659" FT /product="possible secreted serine protease" FT /tb_orthologue="Rv0983" FT misc_feature 243342..243899 FT /colour=0 FT /note="Pfam match to entry PF00089 trypsin, Trypsin, score FT 50.90, E-value 2.7e-15" FT misc_feature 243909..244151 FT /colour=0 FT /note="Pfam match to entry PF00595 PDZ, PDZ domain (Also FT known as DHR or GLGF)., score 16.80, E-value 0.025" FT CDS 244225..244770 FT /class="I.G.4" FT /colour=7 FT /gene="moaB" FT /gene="ML0177" FT /note="Similar to M.tuberculosis moaB2, putative FT molybdenum cofactor biosynthesis protein, TR:O53897 FT (EMBL:AL123456) (181 aa); Fasta score E(): 0, 92.7% FT identity in 179 aa overlap. Similar to many e.g. to the FT C-terminal halves of Arabidopsis thaliana cnx1, FT multifunctional two-domain protein involved in molybdenum FT cofactor biosynthesis, SW:CNX1_ARATH (Q39054) (670 aa); FT Fasta score E(): 7.7e-10, 36.4% identity in 187 aa overlap FT and Synechococcus sp. (strain PCC 7942) moaCB, molybdenum FT cofactor biosynthesis protein, SW:MOCB_SYNP7 (Q56208) (319 FT aa); Fasta score E(): 1.3e-09, 35.9% identity in 142 aa FT overlap. Previously sequenced as TR:Q9Z5G5 (EMBL:AL035500) FT (181 aa); Fasta score E(): 0, 99.4% identity in 181 aa FT overlap." FT /product="putative molybdenum cofactor biosynthesis FT protein" FT /tb_orthologue="moaB2" FT CDS complement(244787..245251) FT /class="II.C.4" FT /colour=3 FT /gene="mscL" FT /gene="ML0178" FT /note="Similar to M.tuberculosis mscL, Rv0985c, putative FT mechanosensitive channel protein, SW:MSCL_MYCTU (O53898) FT (151 aa); Fasta score E(): 0, 71.0% identity in 155 aa FT overlap. Similar to many e.g. Escherichia coli mscL, FT large-conductance mechanosensitive channel, SW:MSCL_ECOLI FT (P23867) (136 aa); Fasta score E(): 1.3e-07, 32.1% FT identity in 134 aa overlap. Previously sequenced as FT SW:MSCL_MYCLE (Q9Z5G4) (154 aa); Fasta score E(): 0, FT 100.0% identity in 154 aa overlap. Contains hydrophobic, FT possible membrane-spanning regions. Contains Pfam match to FT entry PF01741 MscL, Large-conductance mechanosensitive FT channel, MscL. Contains PS01327 Large-conductance FT mechanosensitive channels mscL family signature." FT /product="putative mechanosensitive channel protein" FT /tb_orthologue="Rv0985c" FT misc_feature complement(244868..245191) FT /colour=0 FT /note="Pfam match to entry PF01741 MscL, Large-conductance FT mechanosensitive channel, MscL, score 91.50, E-value FT 1.6e-23" FT misc_feature complement(245180..245221) FT /colour=8 FT /note="PS01327 Large-conductance mechanosensitive channels FT mscL family signature" FT RBS complement(245258..245263) FT /note="possible RBS" FT CDS complement(245324..245983) FT /class="V" FT /colour=0 FT /gene="ML0179" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv0990c (Best blastx score 338)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(246049..246348) FT /class="VI" FT /colour=10 FT /gene="ML0180" FT /note="Similar to M.tuberculosis Rv0991c, hypothetical FT protein with Ser-rich C-terminus, TR:O05574 FT (EMBL:AL123456) (110 aa); Fasta score E(): 1.1e-23, 78.5% FT identity in 93 aa overlap. Similar to other bacterial FT Ser-rich hypothetical proteins e.g. Streptomyces FT coelicolor SCE22.04, hypothetical protein, TR:CAB90971 FT (EMBL:AL355832) (110 aa); Fasta score E(): 2.6e-15, 54.5% FT identity in 99 aa overlap. Previously sequenced as FT TR:Q9Z5G3 (EMBL:AL035500) (120 aa); Fasta score E(): FT 1.1e-32, 100.0% identity in 99 aa overlap." FT /product="conserved hypothetical protein. Ser-rich FT C-terminus" FT /tb_orthologue="Rv0991c" FT RBS complement(246354..246358) FT /note="possible RBS" FT CDS complement(246423..247016) FT /class="V" FT /colour=10 FT /gene="ML0181" FT /note="Similar to M.tuberculosis Rv0992c, conserved FT hypothetical protein, TR:O05575 (EMBL:AL123456) (197 aa); FT Fasta score E(): 0, 72.6% identity in 197 aa overlap. FT Similar to many hypothetical proteins and shows weak FT similarity to methenyltetrahydrofolate synthetases e.g. FT human mthfs, 5-formyltetrahydrofolate cyclo-ligase, FT SW:FTHC_HUMAN (P49914) (202 aa); Fasta score E(): 7e-06, FT 23.3% identity in 189 aa overlap. Previously sequenced as FT TR:Q9Z5G2 (EMBL:AL035500) (197 aa); Fasta score E(): 0, FT 100.0% identity in 197 aa overlap. Contains Pfam match to FT entry PF01812 5-FTHF_cyc-lig, 5-formyltetrahydrofolate FT cyclo-ligase." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv0992c" FT misc_feature complement(246459..246971) FT /colour=0 FT /note="Pfam match to entry PF01812 5-FTHF_cyc-lig, FT 5-formyltetrahydrofolate cyclo-ligase, score 169.90, FT E-value 4.1e-47" FT RBS complement(247034..247037) FT /note="possible RBS" FT CDS 247094..248014 FT /EC_number="2.7.7.9" FT /class="I.A.1" FT /colour=7 FT /gene="galU" FT /gene="ML0182" FT /note="Similar to M.tuberculosis galU, Rv0993, FT UTP-glucose-1-phosphate uridylyltransferase, TR:O05576 FT (EMBL:AL123456) (306 aa); Fasta score E(): 0, 89.7% FT identity in 302 aa overlap. Similar to many e.g. FT Escherichia coli galU, UTP--glucose-1-phosphate FT uridylyltransferase, SW:GALU_ECOLI (P25520) (301 aa); FT Fasta score E(): 5.4e-33, 38.8% identity in 299 aa FT overlap. Previously sequenced as TR:Q9Z5G1 (EMBL:AL035500) FT (306 aa); Fasta score E(): 0, 100.0% identity in 306 aa FT overlap. Contains Pfam match to entry PF00483 FT NTP_transferase, Nucleotidyl transferase." FT /product="putative UTP-glucose-1-phosphate FT uridylyltransferase" FT /tb_orthologue="galU" FT misc_feature 247133..247948 FT /colour=0 FT /note="Pfam match to entry PF00483 NTP_transferase, FT Nucleotidyl transferase, score 54.30, E-value 2.7e-12" FT misc_feature 248102..249361 FT /colour=0 FT /note="Pfam match to entry PF00994 MoCF_biosynth, FT Molybdenum cofactor biosynthesis protein, score 551.80, FT E-value 4.7e-162" FT CDS 248102..249376 FT /class="I.G.4" FT /colour=7 FT /gene="moeA" FT /gene="ML0183" FT /note="Similar to M.tuberculosis moeA, Rv0994, putative FT molybdopterin biosynthesis protein, SW:MOEA_MYCTU (O05577) FT (426 aa); Fasta score E(): 0, 88.3% identity in 426 aa FT overlap. Similar to many e.g. Escherichia coli moeA, FT molybdopterin biosynthesis protein, SW:MOEA_ECOLI (P12281) FT (411 aa); Fasta score E(): 3.3e-23, 31.4% identity in 392 FT aa overlap. Previously sequenced as TR:Q9Z5G0 FT (EMBL:AL035500) (424 aa); Fasta score E(): 0, 99.8% FT identity in 424 aa overlap. Contains Pfam match to entry FT PF00994 MoCF_biosynth, Molybdenum cofactor biosynthesis FT protein." FT /product="putative molybdopterin biosynthesis protein" FT /tb_orthologue="moeA" FT CDS 249407..250051 FT /class="II.A.1" FT /colour=2 FT /gene="rimJ" FT /gene="ML0184" FT /note="Similar to M.tuberculosis rimJ, Rv0995, possible FT acetyltransferase, TR:O05578 (EMBL:AL123456) (203 aa); FT Fasta score E(): 0, 86.0% identity in 200 aa overlap. FT Similar to Escherichia coli rimJ, FT ribosomal-protein-alanine acetyltransferase, SW:RIMJ_ECOLI FT (P09454) (194 aa); Fasta score E(): 9.4e-12, 28.0% FT identity in 189 aa overlap and to other putative FT acetyltransferases. Previously sequenced as TR:Q9Z5F9 FT (EMBL:AL035500) (218 aa); Fasta score E(): 0, 100.0% FT identity in 214 aa overlap. Contains Pfam match to entry FT PF00583 Acetyltransf, Acetyltransferase (GNAT) family." FT /product="possible acetyltransferase" FT /tb_orthologue="rimJ" FT misc_feature 249482..249913 FT /colour=0 FT /note="Pfam match to entry PF00583 Acetyltransf, FT Acetyltransferase (GNAT) family, score 16.40, E-value FT 0.027" FT RBS 250205..250210 FT /note="possible RBS" FT CDS 250221..251249 FT /class="II.C.4" FT /colour=3 FT /gene="ML0185" FT /note="Similar to M.tuberculosis Rv0996, hypothetical FT protein, TR:O05579 (EMBL:AL123456) (358 aa); Fasta score FT E(): 0, 66.4% identity in 360 aa overlap. Previously FT sequenced as TR:Q9Z5F8 (EMBL:AL035500) (342 aa); Fasta FT score E(): 0, 100.0% identity in 342 aa overlap. Contains FT hydrophobic, possible membrane-spanning regions." FT /product="putative membrane protein" FT /tb_orthologue="Rv0996" FT tRNA 251305..251377 FT /colour=4 FT /note="tRNA Ala anticodon CGC, Cove score 82.84" FT CDS 251982..252266 FT /class="V" FT /colour=0 FT /gene="ML0186" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv0997 (Best blastx score 101)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS 252658..253719 FT /class="V" FT /colour=10 FT /gene="ML0187" FT /note="Similar to M. tuberculosis Rv0998, hypothetical FT protein, TR:O05581 (EMBL:AL123456) (333 aa); Fasta score FT E(): 0, 69.7% identity in 330 aa overlap. Contains Pfam FT match to entry PF00027 cNMP_binding, Cyclic FT nucleotide-binding domain. Contains PS00889 Cyclic FT nucleotide-binding domain signature 2." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv0998" FT misc_feature 252817..253098 FT /colour=0 FT /note="Pfam match to entry PF00027 cNMP_binding, Cyclic FT nucleotide-binding domain, score 74.70, E-value 1.9e-18" FT misc_feature 252976..253029 FT /colour=8 FT /note="PS00889 Cyclic nucleotide-binding domain signature FT 2" FT CDS 254028..254291 FT /class="VI" FT /colour=8 FT /gene="ML0188" FT /ml_unique FT /note="Unknown function. Contains PS01047 FT Heavy-metal-associated domain." FT /product="hypothetical protein" FT misc_feature 254181..254273 FT /colour=8 FT /note="PS01047 Heavy-metal-associated domain" FT CDS 254397..255092 FT /class="V" FT /colour=0 FT /gene="ML0189" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv0999 (Best blastx score 400)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS 255245..255751 FT /class="V" FT /colour=0 FT /gene="ML0190" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1000 (Best blastx score = 433)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS 255879..256895 FT /class="I.A.2" FT /colour=0 FT /gene="arcA" FT /gene="ML0191" FT /note="Possible pseudogene of M. tuberculosis orthologue FT arcA (Best blastx score 249)" FT /product="arginine deiminase (pseudogene)" FT /pseudo FT CDS complement(257035..258567) FT /class="II.C.4" FT /colour=3 FT /gene="ML0192" FT /note="Similar to M. tuberculosis Rv1002c, probable FT membrane protein, TR:O05586 (EMBL:AL123456) (503 aa); FT Fasta score E(): 0, 80.4% identity in 510 aa overlap. Also FT similar to Streptomyces coelicolor SCE87.05, putative FT integral membrane protein, TR:Q9RKD3 (EMBL:AL132674) (591 FT aa); Fasta score E(): 0, 36.7% identity in 531 aa overlap. FT Contains hydrophobic, possible membrane-spanning regions." FT /product="putative membrane protein" FT /tb_orthologue="Rv1002c" FT RBS complement(258582..258586) FT /note="possible RBS" FT CDS 258646..259491 FT /class="V" FT /colour=10 FT /gene="ML0193" FT /note="Similar to M. tuberculosis Rv1003, conserved FT hypothetical protein, SW:YA03_MYCTU (O05588) (285 aa); FT Fasta score E(): 0, 74.0% identity in 277 aa overlap. FT Similar to many bacterial hypothetical proteins e.g. FT Streptomyces coelicolor SCE87.04C, conserved hypothetical FT protein, TR:Q9RKD4 (EMBL:AL132674) (286 aa); Fasta score FT E(): 0, 50.5% identity in 277 aa overlap. Contains Pfam FT match to entry PF00590 TP_methylase, Tetrapyrrole FT (Corrin/Porphyrin) Methylases.." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv1003" FT misc_feature 258658..259269 FT /colour=0 FT /note="Pfam match to entry PF00590 TP_methylase, FT Tetrapyrrole (Corrin/Porphyrin) Methylases., score 86.20, FT E-value 6.7e-22" FT repeat_region 259295..260512 FT CDS complement(260507..260906) FT /class="IV.C.1.b" FT /colour=0 FT /gene="PE_PGRS" FT /gene="ML0194" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3653, PE_PGRS (Best blastx score 94)" FT /product="PE_PGRS-family protein (pseudogene)" FT /pseudo FT CDS complement(261342..262320) FT /class="V" FT /colour=0 FT /gene="ML0195" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3651 (Best blastx score 218)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(262754..262939) FT /class="IV.C.1.b" FT /colour=0 FT /gene="PE" FT /gene="ML0196" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3650, PE (Best blastx score 94)" FT /product="PE-family protein (pseudogene)" FT /pseudo FT CDS complement(263238..264560) FT /class="II.A.7" FT /colour=0 FT /gene="ML0197" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3649 (Best blastx score 213)" FT /product="ATP-dependent DNA/RNA helicase (pseudogene)" FT /pseudo FT RBS 264881..264886 FT /note="possible RBS" FT misc_feature 264898..265098 FT /colour=0 FT /note="Pfam match to entry PF00313 CSD, 'Cold-shock' FT DNA-binding domain, score 150.80, E-value 2.4e-41" FT CDS 264898..265101 FT /class="III.E" FT /colour=0 FT /gene="cspA" FT /gene="ML0198" FT /note="Similar to M. tuberculosis cspA, Rv3648c, probable FT cold shock protein, SW:CSPA_MYCTU (O06360) (67 aa); Fasta FT score E(): 3.9e-27, 97.0% identity in 67 aa overlap. FT Similar to many e.g. Arthrobacter globiformis csp, cold FT shock protein, SW:CSP_ARTGO (P54584) (67 aa); Fasta score FT E(): 8.8e-19, 71.6% identity in 67 aa overlap. Previously FT sequenced as TR:O69550 (EMBL:AL023093) (67 aa); Fasta FT score E(): 1.2e-27, 100.0% identity in 67 aa overlap. FT Contains Pfam match to entry PF00313 CSD, 'Cold-shock' FT DNA-binding domain. Contains PS00352 'Cold-shock' FT DNA-binding domain signature." FT /note="Similar to ML2147" FT /product="putative cold shock protein" FT /tb_orthologue="cspA" FT misc_feature 264940..264999 FT /colour=8 FT /note="PS00352 'Cold-shock' DNA-binding domain signature" FT CDS 265213..265815 FT /class="V" FT /colour=10 FT /gene="ML0199" FT /note="Similar to M. tuberculosis Rv3647c, hypothetical FT protein, TR:O06361 (EMBL:AL123456) (192 aa); Fasta score FT E(): 0, 80.4% identity in 199 aa overlap. Previously FT sequenced as TR:O69549 (EMBL:AL023093) (200 aa); Fasta FT score E(): 0, 99.5% identity in 200 aa overlap." FT /tb_orthologue="Rv3647c" FT CDS 266033..268876 FT /EC_number="5.99.1.2" FT /class="II.A.5" FT /colour=2 FT /gene="topA" FT /gene="ML0200" FT /note="Similar to M. tuberculosis topA, DNA topoisomerase FT I, SW:TOP1_MYCTU (Q59567) (934 aa); Fasta score E(): 0, FT 84.3% identity in 942 aa overlap. Previously sequenced as FT SW:TOP1_MYCLE (O69548) (947 aa); Fasta score E(): 0, FT 100.0% identity in 947 aa overlap. Contains Pfam match to FT entry PF01131 Topoisom_bac, Prokaryotic DNA topoisomerase. FT Contains Pfam match to entry PF01751 Toprim, Toprim FT domain. Contains PS00396 Prokaryotic DNA topoisomerase I FT active site." FT /product="putative DNA topoisomerase I" FT /tb_orthologue="topA" FT misc_feature 266078..266446 FT /colour=0 FT /note="Pfam match to entry PF01751 Toprim, Toprim domain, FT score 150.70, E-value 2.6e-41" FT misc_feature 266483..267805 FT /colour=0 FT /note="Pfam match to entry PF01131 Topoisom_bac, FT Prokaryotic DNA topoisomerase, score 657.00, E-value FT 9.7e-194" FT misc_feature 267029..267073 FT /colour=8 FT /note="PS00396 Prokaryotic DNA topoisomerase I active FT site" FT CDS complement(268857..270449) FT /class="II.C.4" FT /colour=3 FT /gene="ML0201" FT /note="Similar to M. tuberculosis Rv3645, probable FT transmembrane protein, TR:O06362 (EMBL:AL123456) (549 aa); FT Fasta score E(): 0, 85.3% identity in 530 aa overlap and FT to several other putative membrane proteins from M. FT tuberculosis. Shares regions of similarity with adenylate FT cyclases e.g. Anabaena sp. (strain PCC 7120) cyaA, FT adenylate cyclase, TR:P94180 (EMBL:D89622) (735 aa); Fasta FT score E(): 1.5e-16, 33.3% identity in 228 aa overlap. FT Previously sequenced as TR:O69547 (EMBL:AL023093) (530 FT aa); Fasta score E(): 0, 99.8% identity in 530 aa overlap. FT Contains hydrophobic, possible membrane-spanning regions. FT Contains Pfam match to entry PF00672 DUF5, Domain found in FT bacterial signal proteins. Contains 2 Pfam matches to FT entry PF00211 guanylate_cyc, Adenylate and Guanylate FT cyclase catalytic domain." FT /product="putative membrane protein" FT /tb_orthologue="Rv3645" FT misc_feature complement(269052..269162) FT /colour=0 FT /note="Pfam match to entry PF00211 guanylate_cyc, FT Adenylate and Guanylate cyclase catalytic domain, score FT 25.40, E-value 1.3e-05" FT misc_feature complement(269247..269450) FT /colour=0 FT /note="Pfam match to entry PF00211 guanylate_cyc, FT Adenylate and Guanylate cyclase catalytic domain, score FT 31.20, E-value 3.4e-07" FT misc_feature complement(269526..269735) FT /colour=0 FT /note="Pfam match to entry PF00672 DUF5, Domain found in FT bacterial signal proteins, score 63.60, E-value 4.3e-15" FT CDS 270605..271822 FT /class="V" FT /colour=10 FT /gene="ML0202" FT /note="Similar to M. tuberculosis Rv3644c, hypothetical FT protein, TR:O06363 (EMBL:AL123456) (401 aa); Fasta score FT E(): 0, 84.4% identity in 404 aa overlap. Similar to the FT N-termini of many DNA polymerase III subunits e.g. FT Escherichia coli holB, DNA polymerase III, delta' subunit, FT SW:HOLB_ECOLI (P28631) (334 aa); Fasta score E(): 2.9e-13, FT 35.1% identity in 205 aa overlap. Previously sequenced as FT TR:O69546 (EMBL:AL023093) (405 aa); Fasta score E(): 0, FT 100.0% identity in 405 aa overlap." FT /note="Similar to the N-terminus of ML2335" FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv3644c" FT tRNA 271901..271976 FT /colour=4 FT /note="tRNA Thr anticodon CGT, Cove score 85.20" FT CDS complement(273290..274999) FT /class="II.C.4" FT /colour=3 FT /gene="ML0203" FT /note="Similar to M. tuberculosis Rv3635, probable FT membrane protein, TR:O06372 (EMBL:AL123456) (591 aa); FT Fasta score E(): 0, 77.2% identity in 569 aa overlap. FT Previously sequenced as TR:O69545 (EMBL:AL023093) (569 FT aa); Fasta score E(): 0, 99.8% identity in 569 aa overlap. FT Contains hydrophobic, possible membrane-spanning regions." FT /product="putative membrane protein" FT /tb_orthologue="Rv3635" FT CDS 275049..276008 FT /class="I.C.3" FT /colour=7 FT /gene="rmlB2" FT /gene="ML0204" FT /note="Similar to M. tuberculosis rmlB2, possible FT dTDP-Glucose 4,6-dehydratase, TR:O06373 (EMBL:AL123456) FT (314 aa); Fasta score E(): 0, 86.1% identity in 309 aa FT overlap. Similar to bacterial sugar-nucleotide FT dehydratases (many putative) e.g. Streptomyces venezuelae FT desIV, TDP-glucose-4,6-dehydratase, TR:Q9ZGH3 FT (EMBL:AF079762) (337 aa); Fasta score E(): 1.4e-24, 34.3% FT identity in 321 aa overlap. Previously sequenced as FT TR:O69544 (EMBL:AL023093) (319 aa); Fasta score E(): 0, FT 100.0% identity in 319 aa overlap. Contains Pfam match to FT entry PF01370 Epimerase, NAD dependent FT epimerase/dehydratase family. Contains PS00061 Short-chain FT dehydrogenases/reductases family signature." FT /note="Similar to ML1964" FT /product="putative sugar-nucleotide dehydratase" FT /tb_orthologue="rmlB2" FT misc_feature 275070..275999 FT /colour=0 FT /note="Pfam match to entry PF01370 Epimerase, NAD FT dependent epimerase/dehydratase family, score 276.90, FT E-value 2.7e-79" FT misc_feature 275460..275546 FT /colour=8 FT /note="PS00061 Short-chain dehydrogenases/reductases FT family signature" FT repeat_region 275744..276923 FT CDS complement(276707..277777) FT /class="II.C.4" FT /colour=3 FT /gene="ML0205" FT /note="Similar to M. tuberculosis Rv3629c, possible FT membrane protein, TR:O06378 (EMBL:AL123456) (365 aa); FT Fasta score E(): 0, 66.2% identity in 361 aa overlap. FT Similar to other bacterial hypothetical membrane proteins FT e.g. Streptomyces coelicolor SCC8A.24C, putative integral FT membrane protein, TR:CAB92842 (EMBL:AL356892) (380 aa); FT Fasta score E(): 1e-21, 47.5% identity in 377 aa overlap. FT Previously sequenced as TR:O69543 (EMBL:AL023093) (356 FT aa); Fasta score E(): 0, 100.0% identity in 356 aa FT overlap. Contains hydrophobic, possible membrane-spanning FT regions." FT /product="putative membrane protein" FT /tb_orthologue="Rv3629c" FT RBS complement(277782..277788) FT /note="possible RBS" FT CDS 277944..279039 FT /class="II.C.4" FT /colour=0 FT /gene="ML0206" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3630 (Best blastx score 392)" FT /product="unknown membrane protein (pseudogene)" FT /pseudo FT CDS 279051..279770 FT /class="IV.H" FT /colour=7 FT /gene="ML0207" FT /note="Similar to M. tuberculosis Rv3631, possible FT glycosyltransferase, TR:O06376 (EMBL:AL123456) (241 aa); FT Fasta score E(): 0, 80.8% identity in 239 aa overlap. FT Similar to many bacterial hypothetical proteins. FT Previously sequenced as TR:O69542 (EMBL:PS50167) (239 aa); FT Fasta score E(): 0, 99.6% identity in 239 aa overlap. FT Contains Pfam match to entry PF00535 Glycos_transf_2, FT Glycosyl transferases." FT /note="Similar to ML1440" FT /product="putative glycosyltransferase" FT /tb_orthologue="Rv3631" FT misc_feature 279081..279572 FT /colour=0 FT /note="Pfam match to entry PF00535 Glycos_transf_2, FT Glycosyl transferases, score 57.10, E-value 3.8e-13" FT repeat_region 279700..281106 FT RBS 279771..279776 FT /note="possible RBS" FT CDS 279784..280125 FT /class="II.C.4" FT /colour=3 FT /gene="ML0208" FT /note="Similar to M. tuberculosis Rv3632, hypothetical FT protein, TR:O06375 (EMBL:AL123456) (114 aa); Fasta score FT E(): 0, 82.0% identity in 111 aa overlap. Previously FT sequenced as TR:O69541 (EMBL:AL023093) (113 aa); Fasta FT score E(): 0, 100.0% identity in 113 aa overlap. Contains FT hydrophobic, possible membrane-spanning regions." FT /product="putative membrane protein" FT /tb_orthologue="Rv3632" FT CDS complement(280783..281282) FT /class="V" FT /colour=0 FT /gene="ML0209" FT /note="Possible pseudogene similar to M. tuberculosis FT paralogue Rv1748 (Best blastx score 146)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(281543..282031) FT /EC_number="3.6.1.1" FT /class="I.A.4" FT /colour=7 FT /gene="ppa" FT /gene="ML0210" FT /note="Similar to M. tuberculosis ppa, Rv3628, probable FT inorganic pyrophosphatase, SW:IPYR_MYCTU (O06379) (162 FT aa); Fasta score E(): 0, 89.5% identity in 162 aa overlap. FT Similar to many e.g. Sulfolobus acidocaldarius ppa, FT inorganic pyrophosphatase, SW:IPYR_SULAC (P50308) (173 FT aa); Fasta score E(): 1.1e-27, 45.9% identity in 159 aa FT overlap. Previously sequenced as SW:IPYR_MYCLE (O69540) FT (162 aa); Fasta score E(): 0, 99.4% identity in 162 aa FT overlap. Contains Pfam match to entry PF00719 FT Pyrophosphatase, Inorganic pyrophosphatase. Contains FT PS00387 Inorganic pyrophosphatase signature." FT /product="putative inorganic pyrophosphatase" FT /tb_orthologue="ppa" FT misc_feature complement(281555..282022) FT /colour=0 FT /note="Pfam match to entry PF00719 Pyrophosphatase, FT Inorganic pyrophosphatase, score 172.10, E-value 9.3e-48" FT misc_feature complement(281858..281878) FT /colour=8 FT /note="PS00387 Inorganic pyrophosphatase signature" FT RBS complement(282040..282045) FT /note="possible RBS" FT RBS 282147..282152 FT /note="possible RBS" FT CDS 282157..283542 FT /class="II.C.3" FT /colour=3 FT /gene="ML0211" FT /note="Similar to M. tuberculosis Rv3627c, possible FT penicillin-binding protein, TR:O06380 (EMBL:AL123456) (461 FT aa); Fasta score E(): 0, 76.0% identity in 462 aa overlap. FT Shows weak similarity to penicillin-binding proteins e.g. FT Neisseria gonorrhoeae pbp3, penicillin binding protein 3, FT TR:O85665 (EMBL:AF071224) (469 aa); Fasta score E(): FT 4.5e-08, 33.1% identity in 163 aa overlap. Similar to FT putative carboxypeptidases from Streptomyces coelicolor FT e.g. SCE9.15C, possible penicillin-binding protein, FT TR:Q9X8I8 (EMBL:AL049841) (459 aa); Fasta score E(): FT 3.8e-32, 36.3% identity in 435 aa overlap. Previously FT sequenced as TR:O69539 (EMBL:AL023093) (461 aa); Fasta FT score E(): 0, 99.8% identity in 461 aa overlap. Contains a FT possible N-terminal signal sequence. Contains Pfam match FT to entry PF02113 Peptidase_S13, D-Ala-D-Ala FT carboxypeptidase 3 (S13) family." FT /product="putative carboxypeptidase" FT /tb_orthologue="Rv3627c" FT misc_feature 282439..283467 FT /colour=0 FT /note="Pfam match to entry PF02113 Peptidase_S13, FT D-Ala-D-Ala carboxypeptidase 3 (S13) family, score 519.60, FT E-value 2.3e-152" FT CDS 283539..284579 FT /class="V" FT /colour=0 FT /gene="ML0212" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3626c (Best blastx score 1003)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS 284580..285551 FT /class="V" FT /colour=10 FT /gene="mesJ" FT /gene="ML0213" FT /note="Similar to M. tuberculosis mesJ, Rv3625c, conserved FT hypothetical protein, SW:Y0C5_MYCTU (O06382) (323 aa); FT Fasta score E(): 0, 78.0% identity in 327 aa overlap. FT Similar to many e.g. Escherichia coli mesJ, putative cell FT cycle protein, SW:MESJ_ECOLI (P52097) (432 aa); Fasta FT score E(): 3.9e-12, 33.0% identity in 279 aa overlap. FT Previously sequenced as SW:Y0C5_MYCLE (O69538) (323 aa); FT Fasta score E(): 0, 100.0% identity in 323 aa overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="mesJ" FT CDS 285584..286225 FT /EC_number="2.4.2.8" FT /class="I.F.4" FT /colour=7 FT /gene="hpt" FT /gene="ML0214" FT /note="Similar to M. tuberculosis hpt, Rv3624c, probable FT hypoxanthine-guanine phosphoribosyltransferase, FT SW:HPRT_MYCTU (O06383) (216 aa); Fasta score E(): 0, 83.1% FT identity in 207 aa overlap. Similar to many e.g. FT Salmonella typhimurium hprt, hypoxanthine FT phosphoribosyltransferase, TR:O33799 (EMBL:AF008931) (178 FT aa); Fasta score E(): 9.6e-32, 50.9% identity in 169 aa FT overlap. Previously sequenced as TR:O69537 (EMBL:AL023093) FT (213 aa); Fasta score E(): 0, 99.5% identity in 213 aa FT overlap. Contains Pfam match to entry PF00156 FT Pribosyltran, Phosphoribosyl transferase domain. Contains FT PS00103 Purine/pyrimidine phosphoribosyl transferases FT signature." FT /product="putative hypoxanthine phosphoribosyltransferase" FT /tb_orthologue="hpt" FT misc_feature 285665..286147 FT /colour=0 FT /note="Pfam match to entry PF00156 Pribosyltran, FT Phosphoribosyl transferase domain, score 84.60, E-value FT 2e-21" FT misc_feature 285965..286003 FT /colour=8 FT /note="PS00103 Purine/pyrimidine phosphoribosyl FT transferases signature" FT CDS complement(286276..286674) FT /class="II.C.1" FT /colour=0 FT /gene="ML0215" FT /note="Possible pseudogene of M. tuberculosis orthologue FT lpqG (Best blastx score 222)" FT /product="putative lipoprotein (pseudogene)" FT /pseudo FT CDS complement(286829..287302) FT /class="V" FT /colour=0 FT /gene="ML0216" FT /note="Possible pseudogene similar to M. tuberculosis FT paralogue Rv0393 (Best blastx score 102)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT repeat_region 288549..289431 FT CDS 288678..288923 FT /class="VI" FT /colour=8 FT /gene="ML0217" FT /ml_unique FT /note="Unknown function. Possibly the remains of a FT disrupted gene." FT /product="hypothetical protein" FT CDS complement(289829..290215) FT /class="VI" FT /colour=8 FT /gene="ML0218" FT /ml_unique FT /note="Improbable CDS suggested by GC frameplot. Possibly FT the remains of a disrupted gene. Previously sequenced as FT TR:O69534 (EMBL:AL023093) (128 aa); Fasta score E(): 0, FT 99.2% identity in 128 aa overlap." FT /product="hypothetical protein" FT CDS complement(290378..290991) FT /class="I.B.7" FT /colour=0 FT /gene="ML0219" FT /note="Possible pseudogene similar to M. tuberculosis FT paralogue Rv3726 (Best blastx score 114)" FT /product="Putative alcohol dehydrogenase, zinc-type FT (pseudogene)" FT /pseudo FT CDS complement(291435..292461) FT /class="I.B.7" FT /colour=0 FT /gene="ML0220" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3618 (Best blastx score 208)" FT /product="similar bacterial luciferase alpha chains FT (pseudogene)" FT /pseudo FT CDS complement(292471..293353) FT /class="IV.I" FT /colour=0 FT /gene="ML0221" FT /note="Possible pseudogene of M. tuberculosis orthologue FT ephA (Best blastx score 177)" FT /product="putative hydrolase (pseudogene)" FT /pseudo FT CDS 293693..296065 FT /class="III.C" FT /colour=3 FT /gene="ftsH" FT /gene="ML0222" FT /note="Similar to M. tuberculosis ftsH, Rv3610c, possible FT cell division protein, SW:FTSH_MYCTU (P96942) (760 aa); FT Fasta score E(): 0, 87.2% identity in 790 aa overlap. FT Similar to many e.g. Escherichia coli ftsH, cell division FT protein, SW:FTSH_ECOLI (P28691) (644 aa); Fasta score E(): FT 0, 49.3% identity in 611 aa overlap. Previously sequenced FT as TR:O69532 (EMBL:AL023093) (787 aa); Fasta score E(): 0, FT 100.0% identity in 787 aa overlap. Contains hydrophobic, FT possible membrane-spanning regions. Contains Pfam match to FT entry PF01434 Peptidase_M41, Peptidase family M41. FT Contains Pfam match to entry PF00004 AAA, ATPases FT associated with various cellular activities (AAA). FT Contains PS00017 ATP/GTP-binding site motif A (P-loop). FT Contains PS00674 AAA-protein family signature." FT /product="putative integral membrane peptidase" FT /tb_orthologue="ftsH" FT misc_feature 294293..294856 FT /colour=0 FT /note="Pfam match to entry PF00004 AAA, ATPases associated FT with various cellular activities (AAA), score 339.00, FT E-value 5.2e-98" FT misc_feature 294308..294331 FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT misc_feature 294605..294661 FT /colour=8 FT /note="PS00674 AAA-protein family signature" FT misc_feature 294872..295504 FT /colour=0 FT /note="Pfam match to entry PF01434 Peptidase_M41, FT Peptidase family M41, score 413.20, E-value 2.4e-120" FT RBS 296069..296075 FT /note="possible RBS" FT CDS 296082..296699 FT /EC_number="3.5.4.16" FT /class="I.G.2" FT /colour=7 FT /gene="folE" FT /gene="ML0223" FT /note="Similar to M. tuberculosis folE, Rv3609c, GTP FT cyclohydrolase I, SW:GCH1_MYCTU (O06273) (202 aa); Fasta FT score E(): 0, 82.0% identity in 205 aa overlap. Similar to FT many e.g. Bacillus subtilis mtrA, GTP cyclohydrolase I, FT SW:GCH1_BACSU (P19465) (190 aa); Fasta score E(): 0, 59.5% FT identity in 190 aa overlap. Previously sequenced as FT SW:GCH1_MYCLE (O69531) (205 aa); Fasta score E(): 0, FT 100.0% identity in 205 aa overlap. Contains Pfam match to FT entry PF01227 GTP_cyclohydroI, GTP cyclohydrolase I. FT Contains PS00859 GTP cyclohydrolase I signature 1. FT Contains PS00860 GTP cyclohydrolase I signature 2." FT /product="putative GTP cyclohydrolase I" FT /tb_orthologue="folE" FT misc_feature 296145..296693 FT /colour=0 FT /note="Pfam match to entry PF01227 GTP_cyclohydroI, GTP FT cyclohydrolase I, score 343.60, E-value 2.2e-99" FT misc_feature 296319..296369 FT /colour=8 FT /note="PS00859 GTP cyclohydrolase I signature 1" FT misc_feature 296469..296501 FT /colour=8 FT /note="PS00860 GTP cyclohydrolase I signature 2" FT CDS 296696..297550 FT /EC_number="2.5.1.15" FT /class="I.G.2" FT /colour=7 FT /gene="folP2" FT /gene="ML0224" FT /note="Similar to M. tuberculosis folP, Rv3608c, FT dihydropteroate synthase, SW:DHP1_MYCTU (O06274) (280 aa); FT Fasta score E(): 0, 76.8% identity in 284 aa overlap. FT Previously sequenced as TR:O69530 (EMBL:AL023093) (284 FT aa); Fasta score E(): 0, 99.6% identity in 284 aa overlap. FT Mutations in this gene result in diaminodiphenylsulfone FT resistance. Contains Pfam match to entry PF00809 DHPS, FT Dihydropteroate synthase. Contains PS00793 Dihydropteroate FT synthase signature 2." FT /note="Similar to ML1063" FT /product="dihydropteroate synthase" FT /tb_orthologue="folP" FT misc_feature 296720..297502 FT /colour=0 FT /note="Pfam match to entry PF00809 DHPS, Dihydropteroate FT synthase, score 428.10, E-value 7.9e-125" FT misc_feature 296819..296860 FT /colour=8 FT /note="PS00793 Dihydropteroate synthase signature 2" FT CDS 297543..297941 FT /EC_number="4.1.2.25" FT /class="I.G.2" FT /colour=7 FT /gene="folB" FT /gene="ML0225" FT /note="Similar to M. tuberculosis folX, Rv3607c, probable FT dihydroneopterin aldolase, SW:FOLB_MYCTU (O06275) (133 FT aa); Fasta score E(): 0, 74.8% identity in 131 aa overlap. FT Similar to many e.g. Staphylococcus aureus folB, FT dihydroneopterin aldolase, SW:FOLB_STAAU (P56740) (121 FT aa); Fasta score E(): 1.9e-09, 33.9% identity in 121 aa FT overlap. Previously sequenced as SW:FOLB_MYCLE (O69529) FT (132 aa); Fasta score E(): 0, 100.0% identity in 132 aa FT overlap. Contains Pfam match to entry PF02152 FolB, FT Dihydroneopterin aldolase." FT /product="putative dihydroneopterin aldolase" FT /tb_orthologue="folX" FT misc_feature 297555..297893 FT /colour=0 FT /note="Pfam match to entry PF02152 FolB, Dihydroneopterin FT aldolase, score 130.10, E-value 1.8e-36" FT CDS 297941..298516 FT /EC_number="2.7.6.3" FT /class="I.G.2" FT /colour=7 FT /gene="folK" FT /gene="ML0226" FT /note="Similar to M. tuberculosis folK, Rv3607c, probable FT 2-amino-4-hydroxy-6-hydroxymethyldihydropterine FT pyrophosphokinase, SW:HPPK_MYCTU (O06276) (188 aa); Fasta FT score E(): 0, 63.2% identity in 190 aa overlap. Similar to FT others e.g. to the C-terminal half of Streptococcus FT pneumoniae sulD, bifunctional folate synthesis protein FT (includes: dihydroneopterin aldolase and FT 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine FT pyrophosphokinase), SW:SULD_STRPN (P22291) (270 aa); Fasta FT score E(): 1.8e-07, 37.8% identity in 135 aa overlap. FT Previously sequenced as SW:HPPK_MYCLE (O69528) (191 aa); FT Fasta score E(): 0, 100.0% identity in 191 aa overlap. FT Contains Pfam match to entry PF01288 HPPK, FT 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase FT (HPPK). Contains PS00794 FT 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase FT signature." FT /product="putative FT 2-amino-4-hydroxy-6-hydroxymethyldihydropterine FT pyrophosphokinase" FT /tb_orthologue="folK" FT misc_feature 297947..298357 FT /colour=0 FT /note="Pfam match to entry PF01288 HPPK, FT 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase FT (HPPK), score 246.10, E-value 1.5e-70" FT misc_feature 298193..298228 FT /colour=8 FT /note="PS00794 FT 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase FT signature" FT CDS 298516..298992 FT /class="II.C.4" FT /colour=3 FT /gene="ML0227" FT /note="Similar to M. tuberculosis Rv3605c, hypothetical FT protein, TR:O06277 (EMBL:AL123456) (158 aa); Fasta score FT E(): 0, 85.4% identity in 158 aa overlap. Previously FT sequenced as TR:O69527 (EMBL:AL023093) (158 aa); Fasta FT score E(): 0, 100.0% identity in 158 aa overlap. Contains FT hydrophobic, possible membrane-spanning regions." FT /product="putative membrane protein" FT /tb_orthologue="Rv3605c" FT CDS 299138..300436 FT /class="II.C.4" FT /colour=3 FT /gene="ML0228" FT /note="Similar to M. tuberculosis Rv3604c, putative FT membrane protein, TR:O06278 (EMBL:AL123456) (462 aa); FT Fasta score E(): 0, 59.7% identity in 432 aa overlap. FT Previously sequenced as TR:O69526 (EMBL:AL023093) (432 FT aa); Fasta score E(): 0, 100.0% identity in 432 aa FT overlap. Contains hydrophobic, possible membrane-spanning FT regions." FT /product="putative membrane protein" FT /tb_orthologue="Rv3604c" FT RBS 300524..300527 FT /note="possible RBS" FT CDS 300537..301466 FT /class="V" FT /colour=10 FT /gene="ML0229" FT /note="Similar to M. tuberculosis Rv3603c, hypothetical FT protein, TR:O06279 (EMBL:AL123456) (303 aa); Fasta score FT E(): 0, 67.8% identity in 311 aa overlap. Similar to the FT N-terminal half of Streptomyces coelicolor SCE126.02C, FT hypothetical protein, TR:Q9X845 (EMBL:AL049630) (420 aa); FT Fasta score E(): 4.1e-24, 36.7% identity in 294 aa FT overlap. Previously sequenced as TR:O69525 (EMBL:AL023093) FT (309 aa); Fasta score E(): 0, 100.0% identity in 309 aa FT overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv3603c" FT CDS 301463..302404 FT /EC_number="6.3.2.1" FT /class="I.G.5" FT /colour=7 FT /gene="panC" FT /gene="ML0230" FT /note="Similar to M. tuberculosis panC, putative FT pantoate-[beta]-alanine ligase, SW:PANC_MYCTU (O06280) FT (309 aa); Fasta score E(): 0, 82.2% identity in 297 aa FT overlap. Similar to many e.g. Escherichia coli FT pantoate--beta-alanine ligase, SW:PANC_ECOL (P31663) (283 FT aa); Fasta score E(): 0, 46.1% identity in 269 aa overlap. FT Previously sequenced as SW:PANC_MYCLE (O69524) (191 aa); FT Fasta score E(): 0, 100.0% identity in 191 aa overlap." FT /product="putative pantoate-[beta]-alanine ligase" FT /tb_orthologue="panC" FT CDS 302404..302832 FT /EC_number="4.1.1.11" FT /class="I.G.5" FT /colour=7 FT /gene="panD" FT /gene="ML0231" FT /note="Similar to M. tuberculosis panD, putative FT aspartate-1-decarboxylase, SW:PAND_MYCTU (Z95557) (139 FT aa); Fasta score E(): 0, 82.9% identity in 140 aa overlap. FT Similar to Corynebacterium glutamicum panD, FT L-aspartate-alpha-decarboxylase precursor, TR:Q9X4N0 FT (EMBL:AF116184) (136 aa); Fasta score E(): 9.3e-29, 68.8% FT identity in 128 aa overlap." FT /product="putative aspartate-1-decarboxylase" FT /tb_orthologue="panD" FT CDS 302835..303659 FT /class="V" FT /colour=10 FT /gene="ML0232" FT /note="Similar to M. tuberculosis Rv3600c, conserved FT hypothetical protein, TR:O06282 (EMBL:AL123456) (272 aa); FT Fasta score E(): 0, 90.5% identity in 274 aa overlap. FT Similar to otehr bacterial hypothetical proteins e.g. FT Streptomyces coelicolor SCE94.31C, hypothetical protein, FT TR:Q9X8N6 (EMBL:AL049628) (265 aa); Fasta score E(): 0, FT 52.0% identity in 269 aa overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv3600c" FT RBS 303717..303720 FT /note="possible RBS" FT CDS 303740..305263 FT /EC_number="6.1.1.6" FT /class="II.A.3" FT /colour=2 FT /gene="lysS" FT /gene="ML0233" FT /note="Similar to M. tuberculosis lysS, Rv3598c, putative FT lysyl-tRNA synthase, SW:SYK_MYCTU (O06284) (505 aa); Fasta FT score E(): 0, 85.4% identity in 501 aa overlap. Similar to FT Escherichia coli lysU, lysyl-tRNA synthase, SW:SYK2_ECOLI FT (P14825) (504 aa); Fasta score E(): 0, 38.8% identity in FT 497 aa overlap. Contains Pfam match to entry PF00152 FT tRNA-synt_2, tRNA synthetases class II (D, K and N). FT Contains PS00179 Aminoacyl-transfer RNA synthetases FT class-II signature 1." FT /note="Similar to the C-terminal half of ML1393" FT /product="putative lysyl-tRNA synthase" FT /tb_orthologue="lysS" FT misc_feature 304211..305251 FT /colour=0 FT /note="Pfam match to entry PF00152 tRNA-synt_2, tRNA FT synthetases class II (D, K and N), score 375.40, E-value FT 5.6e-109" FT misc_feature 304511..304564 FT /colour=8 FT /note="PS00179 Aminoacyl-transfer RNA synthetases class-II FT signature 1" FT RBS 305356..305359 FT /note="possible RBS" FT CDS 305368..305706 FT /class="II.C.2" FT /colour=3 FT /gene="lsr2" FT /gene="ML0234" FT /note="Similar to M. tuberculosis lsr2, putative lsr FT protein, SW:LSR2_MYCTU (O06285) (112 aa); Fasta score E(): FT 0, 92.9% identity in 112 aa overlap. Homologues also occur FT in Streptomyces coelicolor e.g. Streptomyces coelicolor FT SCE94.26C, putative lsr2-like protein, TR:Q9X8N1 FT (EMBL:AL049628) (111 aa); Fasta score E(): 7.3e-18, 56.3% FT identity in 112 aa overlap. Previously sequenced as SW:." FT /product="lsr2 protein (15 kDa antigen)" FT /tb_orthologue="lsr2" FT RBS 305988..305994 FT /note="possible RBS" FT CDS 306003..308549 FT /class="II.B.3" FT /colour=7 FT /gene="clpC" FT /gene="ML0235" FT /note="Similar to M. tuberculosis clpC, ATP-dependent Clp FT protease, SW:CLPC_MYCTU (O06286) (848 aa); Fasta score FT E(): 0, 97.2% identity in 845 aa overlap. Similar to many FT e.g. Bacillus subtilis clpC, negative regulator of genetic FT competence (clpC-family) and Treponema hyodysenteriae FT tlyB, hemolysin B, SW:HLYB_TREHY (Q54316) (828 aa); Fasta FT score E(): 0, 51.4% identity in 812 aa overlap. Contains FT Pfam match to entry PF00495 clpA_B, Chaperonin clpA/B. FT Contains Pfam match to entry PF02151 UVR, UvrB/uvrC motif. FT Contains 2 x PS00017 ATP/GTP-binding site motif A FT (P-loop)." FT /note="Similar to ML2490" FT /product="putative ATP-dependent Clp protease" FT /tb_orthologue="clpC" FT misc_feature 306648..306671 FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT misc_feature 307275..307382 FT /colour=0 FT /note="Pfam match to entry PF02151 UVR, UvrB/uvrC motif, FT score 39.60, E-value 7e-08" FT misc_feature 307434..308408 FT /colour=0 FT /note="Pfam match to entry PF00495 clpA_B, Chaperonin FT clpA/B, score 647.10, E-value 9.5e-191" FT misc_feature 307659..307682 FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT CDS complement(309643..310899) FT /EC_number="4.1.3.-" FT /class="I.G.2" FT /colour=7 FT /gene="pabB" FT /gene="ML0236" FT /note="Similar to M. tuberculosis pabB, Rv1005c, putative FT para-aminobenzoate synthase component I, TR:O05591 FT (EMBL:Z94752) (458 aa); Fasta score E(): 0, 79.1% identity FT in 412 aa overlap. Similar to many e.g. Escherichia coli FT para-aminobenzoate synthase component I, SW:PABB_ECOLI FT (P05041) (453 aa); Fasta score E(): 5.5e-31, 35.2% FT identity in 378 aa overlap. When compared to the predicted FT M. tuberculosis translation, this CDS is missing the FT N-terminal 30 aa, thus it may be a pseudogene. Contains FT Pfam match to entry PF00425 chorismate_bind, chorismate FT binding enzyme." FT /note="Similar to the C-terminal halves of ML0808 and FT ML1269" FT /product="putative para-aminobenzoate synthase component" FT /tb_orthologue="pabB" FT misc_feature complement(309676..310242) FT /colour=0 FT /note="Pfam match to entry PF00425 chorismate_bind, FT chorismate binding enzyme, score 192.30, E-value 6.7e-55" FT CDS 310900..311580 FT /class="V" FT /colour=0 FT /gene="ML0237" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1006 (Best blastx score 389)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(312208..313821) FT /EC_number="6.1.1.10" FT /class="II.A.3" FT /colour=2 FT /gene="metS" FT /gene="ML0238" FT /note="Similar to M. tuberculosis metS, Rv1007c, FT methionyl-tRNA synthetase, SW:SYM_MYCTU (O05593) (519 aa); FT Fasta score E(): 0, 85.7% identity in 516 aa overlap. FT Similar to many e.g. Bacillus stearothermophilus metG FT (metS), methionyl-tRNA synthetase, SW:SYM_BACST (P23920) FT (649 aa); Fasta score E(): 0, 40.9% identity in 550 aa FT overlap. Contains Pfam match to entry PF00133 tRNA-synt_1, FT tRNA synthetases class I (I, L, M and V). Contains PS00178 FT Aminoacyl-transfer RNA synthetases class-I signature." FT /product="putative methionyl-tRNA synthase" FT /tb_orthologue="metS" FT misc_feature complement(312619..313821) FT /colour=0 FT /note="Pfam match to entry PF00133 tRNA-synt_1, tRNA FT synthetases class I (I, L, M and V), score -186.40, FT E-value 1.5e-07" FT misc_feature complement(313756..313785) FT /colour=8 FT /note="PS00178 Aminoacyl-transfer RNA synthetases class-I FT signature" FT misc_feature 313910..313936 FT /colour=8 FT /note="PS01137 Uncharacterized protein family UPF0006 FT signature 1" FT CDS 313910..314698 FT /class="V" FT /colour=10 FT /gene="ML0239" FT /note="Similar to M. tuberculosis ycfH, Rv1008, conserved FT hypothetical protein, TR:O08343 (EMBL:AL123456) (264 aa); FT Fasta score E(): 0, 82.3% identity in 260 aa overlap. FT Similar to many e.g. Bacillus subtilis yabD, conserved FT hypothetical protein, SW:YABD_BACSU (P37545) (255 aa); FT Fasta score E(): 0, 41.0% identity in 261 aa overlap. FT Contains Pfam match to entry PF01026 UPF0006, FT Metalloenzyme of unknown function. Contains PS01137 FT Uncharacterized protein family UPF0006 signature 1. FT Contains PS01091 Uncharacterized protein family UPF0006 FT signature 3." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv1008" FT misc_feature 313925..314683 FT /colour=0 FT /note="Pfam match to entry PF01026 UPF0006, Metalloenzyme FT of unknown function, score 348.40, E-value 7.8e-101" FT misc_feature 314489..314539 FT /colour=8 FT /note="PS01091 Uncharacterized protein family UPF0006 FT signature 3" FT RBS 314928..314932 FT /note="possible RBS" FT CDS 314948..316075 FT /class="V" FT /colour=10 FT /gene="ML0240" FT /note="Similar to M. tuberculosis Rv1009, conserved FT hypothetical protein, TR:O05594 (EMBL:AL123456) (362 aa); FT Fasta score E(): 0, 82.3% identity in 361 aa overlap. The FT C-terminus is similar to a region conserved in many FT bacterial hypothetical proteins e.g. Streptomyces FT coelicolor SCE87.01C, hypothetical protein, TR:Q9RKD7 FT (EMBL:AL132674) (330 aa); Fasta score E(): 3.2e-14, 33.8% FT identity in 225 aa overlap." FT /note="C-terminus is similar to region of ML2030 and FT ML2151" FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv1009" FT RBS 316026..316030 FT /note="possible RBS" FT CDS 316048..316968 FT /EC_number="2.1.1.-" FT /class="II.A.2" FT /colour=2 FT /gene="ksgA" FT /gene="ML0241" FT /note="Similar to M. tuberculosis ksgA, dimethyladenosine FT transferase, TR:O05595 (EMBL:AL123456) (317 aa); Fasta FT score E(): 0, 85.0% identity in 294 aa overlap. Similar to FT many e.g. Escherichia coli ksgA, dimethyladenosine FT transferase, SW:KSGA_ECOLI (M68521) (273 aa); Fasta score FT E(): 8.3e-23, 33.7% identity in 261 aa overlap. Contains FT Pfam match to entry PF00398 RrnaAD, Ribosomal RNA adenine FT dimethylases. Contains PS01131 Ribosomal RNA adenine FT dimethylases signature." FT /product="putative dimethyladenosine transferase" FT /tb_orthologue="ksgA" FT misc_feature 316153..316917 FT /colour=0 FT /note="Pfam match to entry PF00398 RrnaAD, Ribosomal RNA FT adenine dimethylases, score 151.60, E-value 2e-46" FT misc_feature 316225..316308 FT /colour=8 FT /note="PS01131 Ribosomal RNA adenine dimethylases FT signature" FT CDS 317042..317977 FT /EC_number="2.7.1.-" FT /class="I.C.1" FT /colour=7 FT /gene="ML0242" FT /note="Similar to M. tuberculosis ipk, Rv1011, putative FT isopentenyl monophosphate kinase, SW:IPK_MYCTU (O05596) FT (306 aa); Fasta score E(): 0, 87.5% identity in 303 aa FT overlap. Similar to many e.g. Escherichia coli isopentenyl FT monophosphate kinase, SW:IPK_ECOLI (P24209) (283 aa); FT Fasta score E(): 1.3e-13, 35.6% identity in 202 aa FT overlap." FT /product="putative isopentenyl monophosphate kinase" FT /tb_orthologue="Rv1011" FT RBS 318210..318215 FT /note="possible RBS" FT CDS 318220..319854 FT /class="I.I" FT /colour=1 FT /gene="ML0243" FT /note="Similar to several putative acyl-CoA synthases from FT M.tuberculosis e.g. pks16, Rv1013, possible polyketide FT synthase, TR:O05598 (EMBL:AL123456) (544 aa); Fasta score FT E(): 0, 82.2% identity in 544 aa overlap. Similar to FT domains of polyketide synthases e.g. Bacillus subtilis FT mycA, mycosubtilin synthetase, TR:Q9R9J1 (EMBL:AF184956) FT (3971 aa); Fasta score E(): 0, 33.7% identity in 404 aa FT overlap. Contains Pfam match to entry PF00501 AMP-binding, FT AMP-binding enzyme. Contains PS00455 Putative AMP-binding FT domain signature." FT /note="Similar to ML0887, ML0100, ML0132, ML0138, ML1051, FT ML1234, ML1994, ML2358 and ML2546" FT /product="putative acyl-CoA synthetase" FT /tb_orthologue="pks16" FT misc_feature 318310..319623 FT /colour=0 FT /note="Pfam match to entry PF00501 AMP-binding, FT AMP-binding enzyme, score -49.80, E-value 1.2e-12" FT misc_feature 318688..318723 FT /colour=8 FT /note="PS00455 Putative AMP-binding domain signature" FT CDS complement(319965..320564) FT /EC_number="3.1.1.29" FT /class="II.B.1" FT /colour=7 FT /gene="pth" FT /gene="ML0244" FT /note="Similar to M. tuberculosis pth, Rv1014c, FT peptidyl-tRNA hydrolase, SW:PTH_MYCTU (P96386) (191 aa); FT Fasta score E(): 0, 77.7% identity in 188 aa overlap. FT Similar to many e.g. Escherichia coli pth, peptidyl-tRNA FT hydrolase, SW:PTH_ECOLI (P23932) (194 aa); Fasta score FT E(): 3.7e-22, 37.2% identity in 188 aa overlap. Contains FT Pfam match to entry PF01195 Pept_tRNA_hydro, Peptidyl-tRNA FT hydrolase. Contains PS01196 Peptidyl-tRNA hydrolase FT signature 2." FT /product="putative peptidyl-tRNA hydrolase" FT /tb_orthologue="pth" FT misc_feature complement(319974..320534) FT /colour=0 FT /note="Pfam match to entry PF01195 Pept_tRNA_hydro, FT Peptidyl-tRNA hydrolase, score 297.90, E-value 1.2e-85" FT misc_feature complement(320178..320210) FT /colour=8 FT /note="PS01196 Peptidyl-tRNA hydrolase signature 2" FT CDS complement(320577..321224) FT /class="II.A.1" FT /colour=2 FT /gene="rplY" FT /gene="ML0245" FT /note="Similar to M. tuberculosis rplY, 50S ribosomal FT protein L25, SW:RL25_MYCTU (P96385) (215 aa); Fasta score FT E(): 0, 73.9% identity in 218 aa overlap. Similar to many FT e.g. Escherichia coli rplY, 50S ribosomal protein L25, FT SW:RL25_ECOLI (P02426) (94 aa); Fasta score E(): 0.00054, FT 33.7% identity in 89 aa overlap. Contains Pfam match to FT entry PF01386 Ribosomal_L25p, Ribosomal L25p family." FT /product="putative 50S ribosomal protein L25" FT /tb_orthologue="rplY" FT misc_feature complement(320940..321200) FT /colour=0 FT /note="Pfam match to entry PF01386 Ribosomal_L25p, FT Ribosomal L25p family, score 149.60, E-value 5.4e-41" FT RBS complement(321232..321237) FT /note="possible RBS" FT CDS complement(322399..323055) FT /class="II.C.1" FT /colour=3 FT /gene="lpqT" FT /gene="ML0246" FT /note="Similar to M. tuberculosis lpqT, putative FT lipoprotein, SW:LPQT_MYCTU (P96384) (226 aa); Fasta score FT E(): 0, 67.1% identity in 213 aa overlap. Also similar to FT M. tuberculosis MTC28, Rv0040c, proline rich 28 kDa FT antigen precursor, SW:PR28_MYCTU (P71697) (311 aa); Fasta FT score E(): 1.3e-10, 33.3% identity in 159 aa overlap. FT Contains a probable N-terminal signal sequence. Contains FT PS00013 Prokaryotic membrane lipoprotein lipid attachment FT site." FT /note="Similar to ML0031, except at N-terminus" FT /product="putative lipoprotein" FT /tb_orthologue="lpqT" FT misc_feature complement(322984..323016) FT /colour=8 FT /note="PS00013 Prokaryotic membrane lipoprotein lipid FT attachment site" FT CDS complement(323092..323406) FT /class="III.F" FT /colour=0 FT /gene="arsC" FT /gene="ML0247" FT /ml_unique FT /note="Similar to many e.g. Escherichia coli arsC, FT arsenate reductase, SW:ARC1_ECOLI (P08692) (141 aa); Fasta FT score E(): 1.7e-07, 38.8% identity in 103 aa overlap. FT Similar to proteins which expand the substrate specificity FT of arsAB pump." FT /product="putative arsenate reductase" FT CDS complement(323462..324445) FT /class="I.F.1" FT /colour=7 FT /gene="prsA" FT /gene="ML0248" FT /note="Similar to M. tuberculosis prsA, ribose-phosphate FT pyrophosphokinase, TR:P96383 (EMBL:AL123456) (326 aa); FT Fasta score E(): 0, 92.0% identity in 326 aa overlap. FT Similar to many e.g. Bacillus subtilis prs, FT ribose-phosphate pyrophosphokinase, SW:KPRS_BACSU (P14193) FT (317 aa); Fasta score E(): 0, 45.8% identity in 323 aa FT overlap. Contains Pfam match to entry PF00156 FT Pribosyltran, Phosphoribosyl transferase domain. Contains FT PS00103 Purine/pyrimidine phosphoribosyl transferases FT signature." FT /product="putative ribose-phosphate pyrophosphokinase" FT /tb_orthologue="prsA" FT misc_feature complement(323594..324016) FT /colour=0 FT /note="Pfam match to entry PF00156 Pribosyltran, FT Phosphoribosyl transferase domain, score 95.00, E-value FT 1.5e-24" FT misc_feature complement(323729..323767) FT /colour=8 FT /note="PS00103 Purine/pyrimidine phosphoribosyl FT transferases signature" FT RBS complement(324451..324454) FT /note="possible RBS" FT CDS complement(324556..326034) FT /EC_number="2.7.7.23" FT /class="II.C.3" FT /colour=3 FT /gene="glmU" FT /gene="ML0249" FT /note="Similar to M. tuberculosis glmU, putative FT UDP-N-acetylglucosamine pyrophosphorylase, TR:P96382 FT (EMBL:AL123456) (495 aa); Fasta score E(): 0, 82.1% FT identity in 492 aa overlap. Similar to many e.g. Neisseria FT gonorrhoeae glmU, UDP-N-acetylglucosamine FT pyrophosphorylase, SW:GLMU_NEIGO (Q50986) (456 aa); Fasta FT score E(): 0, 37.4% identity in 462 aa overlap. Contains FT Pfam match to entry PF00483 NTP_transferase, Nucleotidyl FT transferase. Contains 5 Pfam matches to entry PF00132 FT hexapep, Bacterial transferase hexapeptide (four FT repeats)." FT /product="putative UDP-N-acetylglucosamine FT pyrophosphorylase" FT /tb_orthologue="glmU" FT misc_feature complement(324715..324768) FT /colour=0 FT /note="Pfam match to entry PF00132 hexapep, Bacterial FT transferase hexapeptide (four repeats), score 9.10, FT E-value 44" FT misc_feature complement(324895..324948) FT /colour=0 FT /note="Pfam match to entry PF00132 hexapep, Bacterial FT transferase hexapeptide (four repeats), score 8.60, FT E-value 53" FT misc_feature complement(325000..325053) FT /colour=0 FT /note="Pfam match to entry PF00132 hexapep, Bacterial FT transferase hexapeptide (four repeats), score 11.30, FT E-value 23" FT misc_feature complement(325099..325152) FT /colour=0 FT /note="Pfam match to entry PF00132 hexapep, Bacterial FT transferase hexapeptide (four repeats), score 13.10, FT E-value 6.6" FT misc_feature complement(325153..325206) FT /colour=0 FT /note="Pfam match to entry PF00132 hexapep, Bacterial FT transferase hexapeptide (four repeats), score 17.00, FT E-value 0.44" FT misc_feature complement(325303..326013) FT /colour=0 FT /note="Pfam match to entry PF00483 NTP_transferase, FT Nucleotidyl transferase, score -0.80, E-value 1.3e-07" FT RBS complement(326040..326044) FT /note="possible RBS" FT tRNA complement(326132..326203) FT /colour=4 FT /note="tRNA Gln anticodon TTG, Cove score 52.56" FT CDS 326387..326995 FT /class="I.J.1" FT /colour=0 FT /gene="ML0250" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1019 (Best blastx score 404)" FT /product="transcriptional regulator (TetR/AcrR family) FT (pseudogene)" FT /pseudo FT CDS complement(327120..327945) FT /class="IV.I" FT /colour=0 FT /gene="ML0251" FT /note="Possible pseudogene similar to M. tuberculosis FT paralogue lysA (Best blastx score 114)" FT /product="putative decarboxylase (pseudogene)" FT /pseudo FT CDS 328067..331741 FT /class="II.A.5" FT /colour=2 FT /gene="mfd" FT /gene="ML0252" FT /note="Similar to M. tuberculosis mfd, FT transcription-repair coupling factor, SW:MFD_MYCTU FT (P96380) (1234 aa); Fasta score E(): 0, 84.3% identity in FT 1229 aa overlap. Similar to many e.g. Escherichia coli FT mfd, transcription-repair coupling factor, SW:MFD_ECOLI FT (P30958) (1148 aa); Fasta score E(): 0, 38.1% identity in FT 1111 aa overlap. Contains Pfam match to entry PF00271 FT helicase_C, Helicases conserved C-terminal domain. FT Contains Pfam match to entry PF00270 DEAD, DEAD/DEAH box FT helicase." FT /product="putative transcription-repair coupling factor" FT /tb_orthologue="mfd" FT misc_feature 329996..330583 FT /colour=0 FT /note="Pfam match to entry PF00270 DEAD, DEAD/DEAH box FT helicase, score 73.60, E-value 2.2e-22" FT misc_feature 330692..330943 FT /colour=0 FT /note="Pfam match to entry PF00271 helicase_C, Helicases FT conserved C-terminal domain, score 63.10, E-value 6.1e-15" FT CDS 331780..332529 FT /class="V" FT /colour=0 FT /gene="ML0253" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1021 (Best blastx score 252)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS 332923..333664 FT /class="II.C.1" FT /colour=0 FT /gene="lpqU" FT /gene="ML0254" FT /note="Possible pseudogene of M. tuberculosis orthologue FT lpqU (Best blastx score 224)" FT /product="lipoprotein (pseudogene)" FT /pseudo FT RBS 333730..333734 FT /note="possible RBS" FT CDS 333751..335094 FT /EC_number="4.2.1.11" FT /class="I.B.1" FT /colour=7 FT /gene="eno" FT /gene="ML0255" FT /note="Similar to M. tuberculosis eno, putative enolase, FT SW:ENO_MYCTU (P96377) (429 aa); Fasta score E(): 0, 88.8% FT identity in 429 aa overlap. Similar to many e.g. Bacillus FT subtilis eno, enolase (2-phosphoglycerate dehydratase), FT SW:ENO_BACSU (P37869) (430 aa); Fasta score E(): 0, 58.8% FT identity in 425 aa overlap. Contains Pfam match to entry FT PF00113 enolase, Enol-ase. Contains PS00164 Enolase FT signature." FT /product="putative enolase" FT /tb_orthologue="eno" FT misc_feature 333808..335076 FT /colour=0 FT /note="Pfam match to entry PF00113 enolase, Enol-ase, FT score 773.50, E-value 8.3e-229" FT misc_feature 334798..334839 FT /colour=8 FT /note="PS00164 Enolase signature" FT RBS 335108..335111 FT /note="possible RBS" FT CDS 335129..335812 FT /class="II.C.4" FT /colour=3 FT /gene="ML0256" FT /note="Similar to M. tuberculosis Rv1024, hypothetical FT protein, TR:P96376 (EMBL:AL123456) (228 aa); Fasta score FT E(): 0, 70.5% identity in 220 aa overlap. Contains FT hydrophobic, possible membrane-spanning region." FT /product="putative membrane protein" FT /tb_orthologue="Rv1024" FT CDS 335805..336308 FT /class="V" FT /colour=10 FT /gene="ML0257" FT /note="Similar to M. tuberculosis Rv1025, hypothetical FT protein, TR:P96375 (EMBL:AL123456) (155 aa); Fasta score FT E(): 0, 85.1% identity in 154 aa overlap. Also similar to FT hypothetical proteins from Thermotoga maritima e.g. FT TM1078, hypothetical protein, TR:Q9X0G7 (EMBL:AE001768) FT (170 aa); Fasta score E(): 2.4e-09, 32.6% identity in 172 FT aa overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv1025" FT RBS 336291..336295 FT /note="possible RBS" FT CDS 336305..337258 FT /class="V" FT /colour=10 FT /gene="ML0258" FT /note="Similar to M. tuberculosis Rv1026, hypothetical FT protein, TR:P96374 (EMBL:AL123456) (319 aa); Fasta score FT E(): 0, 77.6% identity in 321 aa overlap. Similar to many FT bacterial hypothetical proteins and shows weak similarity FT to N-terminal half of Escherichia coli ppx, FT exopolyphosphatase, SW:PPX_ECOLI (P29014) (512 aa); Fasta FT score E(): 6.8e-07, 25.3% identity in 297 aa overlap." FT /note="Shows weak similarity to ML2434" FT /product="hypothetical protein" FT /tb_orthologue="Rv1026" FT CDS 337531..339287 FT /class="II.C.4" FT /colour=0 FT /gene="ML0259" FT /note="Possible pseudogene similar to M. tuberculosis FT paralogue mmpL4 (Best blastx score 308)" FT /product="conserved large membrane protein (pseudogene)" FT /pseudo FT CDS complement(339324..340503) FT /class="I.J.2" FT /colour=0 FT /gene="ML0260" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1032c (Best blastx score 284)" FT /product="sensor histidine kinase (pseudogene)" FT /pseudo FT CDS complement(340549..341173) FT /class="I.J.2" FT /colour=0 FT /gene="ML0261" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1033c (Best blastx score 192)" FT /product="two-component response regulator (pseudogene)" FT /pseudo FT CDS complement(341956..342250) FT /class="V" FT /colour=0 FT /gene="ML0262" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1038c (Best blastx score 310)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(343372..343984) FT /class="IV.C.1.a" FT /colour=0 FT /gene="PE" FT /gene="ML0263" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1040c, PE (Best blastx score 122)" FT /product="PE-family protein (pseudogene)" FT /pseudo FT tRNA 344340..344413 FT /colour=4 FT /note="tRNA Leu anticodon TAA, Cove score 63.13" FT CDS complement(344904..345275) FT /class="V" FT /colour=0 FT /gene="ML0264" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2775 (Best blastx score 95)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS 345708..345920 FT /class="VI" FT /colour=8 FT /gene="ML0265" FT /ml_unique FT /note="Improbable CDS suggested by GC frameplot. Possibly FT the remains of a disrupted gene." FT /product="hypothetical protein" FT CDS 346709..347435 FT /class="V" FT /colour=0 FT /gene="ML0266" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1056 (Best blastx score 289)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT repeat_region 348334..349616 FT CDS complement(350965..351892) FT /class="I.B.6.c" FT /colour=0 FT /gene="ackA" FT /gene="ML0267" FT /note="Possible pseudogene of M. tuberculosis orthologue FT ackA (Best blastx score 257)" FT /product="acetate kinase (pseudogene)" FT /pseudo FT CDS complement(351893..352999) FT /class="I.A.1" FT /colour=0 FT /gene="pta" FT /gene="ML0268" FT /note="Possible pseudogene of M. tuberculosis orthologue FT pta (Best blastx score 310)" FT /product="phosphate acetyltransferase (pseudogene)" FT /pseudo FT CDS complement(353007..354017) FT /class="IV.G" FT /colour=7 FT /gene="ML0269" FT /note="Similar to M. tuberculosis Rv0407, probable FT coenzyme F420-dependent enzyme, TR:P96253 (EMBL:AL123456) FT (336 aa); Fasta score E(): 0, 89.3% identity in 336 aa FT overlap. Similar to mycobacterial G6PDHs e.g. FT Mycobacterium avium fgd, F420-dependent FT glucose-6-phosphate dehydrogenase, TR:Q9XC15 FT (EMBL:AF152394) (336 aa); Fasta score E(): 0, 88.4% FT identity in 336 aa overlap. previously sequenced as FT TR:O69589 (EMBL:AL023514) (336 aa); Fasta score E(): 0, FT 100.0% identity in 336 aa overlap." FT /note="Shows weak similarity to ML0131" FT /product="putative F420-dependent glucose-6-phosphate FT dehydrogenase" FT /tb_orthologue="Rv0407" FT RBS complement(354030..354034) FT /note="possible RBS" FT CDS 354105..354860 FT /class="V" FT /colour=10 FT /gene="ML0270" FT /note="Similar to M. tuberculosis Rv0406c, hypothetical FT protein, TR:O86336 (EMBL:AL123456) (272 aa); Fasta score FT E(): 0, 74.9% identity in 243 aa overlap. Shows similarity FT to members of the metallo-beta-lactamase superfamily. FT Conserved in mycobacteria e.g. Mycobacterium avium bllP, FT beta-lactamase-like protein, TR:Q9XC16 (EMBL:AF152394) FT (247 aa); Fasta score E(): 0, 73.7% identity in 243 aa FT overlap. Previously sequenced as TR:O69590 (EMBL:AL023514) FT (251 aa); Fasta score E(): 0, 100.0% identity in 251 aa FT overlap. Contains Pfam match to entry PF00753 lactamase_B, FT Metallo-beta-lactamase superfamily." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv0406c" FT misc_feature 354150..354785 FT /colour=0 FT /note="Pfam match to entry PF00753 lactamase_B, FT Metallo-beta-lactamase superfamily, score 145.40, E-value FT 9.7e-40" FT CDS complement(354849..355220) FT /class="II.C.4" FT /colour=3 FT /gene="ML0271" FT /note="Similar to M. tuberculosis Rv0401, putative FT membrane protein, TR:P95210 (EMBL:AL123456) (123 aa); FT Fasta score E(): 1.9e-32, 66.9% identity in 121 aa FT overlap. Similar to region of Arabidopsis thaliana FT F27F5.3, hypothetical protein, TR:AAF69175 (EMBL:AC007915) FT (261 aa); Fasta score E(): 1.5e-06, 30.8% identity in 120 FT aa overlap. Previously sequenced as TR:O69591 FT (EMBL:AL023514) (122 aa); Fasta score E(): 0, 100.0% FT identity in 122 aa overlap. Contains hydrophobic, possible FT membrane-spanning regions." FT /product="putative membrane protein" FT /tb_orthologue="Rv0401" FT CDS 355303..355922 FT /class="V" FT /colour=0 FT /gene="ML0272" FT /note="Possible oxidoreductase pseudogene. Similar to FT several M. tuberculosis genes e.g. Rv1941, Rv2214c, FT Rv2857c (Best blastx score 134)" FT /product="putative oxidoreductase (pseudogene)" FT /pseudo FT CDS 355941..356489 FT /class="I.A.3" FT /colour=0 FT /gene="fadE7" FT /gene="ML0273" FT /note="Possible pseudogene of M. tuberculosis orthologue FT fadE7 (Best blastx score 140)" FT /product="acyl-CoA dehydrogenase (pseudogene)" FT /pseudo FT CDS 356520..356618 FT /class="V" FT /colour=0 FT /gene="ML0274" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv0398c (Best blastx score 86)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(357557..358777) FT /EC_number="4.2.99.-" FT /class="I.D.2" FT /colour=7 FT /gene="metZ" FT /gene="ML0275" FT /note="Similar to M. tuberculosis metZ, Rv0391, FT o-succinylhomoserine sulfhydrylase, TR:P95199 FT (EMBL:AL123456) (406 aa); Fasta score E(): 0, 87.9% FT identity in 406 aa overlap. Similar to many e.g. FT Pseudomonas aeruginosa metZ, o-succinylhomoserine FT sulfhydrylase, SW:METZ_PSEAE (P55218) (403 aa); Fasta FT score E(): 0, 44.3% identity in 406 aa overlap. Previously FT sequenced as TR:O69592 (EMBL:AL023514) (426 aa); Fasta FT score E(): 0, 100.0% identity in 406 aa overlap. Contains FT Pfam match to entry PF01053 Cys_Met_Meta_PP, Cys/Met FT metabolism PLP-dependent enzyme." FT /note="Similar to ML2394" FT /product="putative o-succinylhomoserine sulfhydrylase" FT /tb_orthologue="metZ" FT misc_feature complement(357560..358720) FT /colour=0 FT /note="Pfam match to entry PF01053 Cys_Met_Meta_PP, FT Cys/Met metabolism PLP-dependent enzyme, score 638.40, FT E-value 3.8e-188" FT RBS complement(358789..358792) FT /note="possible RBS" FT CDS complement(358834..359277) FT /class="V" FT /colour=10 FT /gene="ML0276" FT /note="Similar to M. tuberculosis Rv0390, hypothetical FT protein, TR:P95198 (AL123456) (140 aa); Fasta score E(): FT 0, 79.0% identity in 138 aa overlap. Also similar to FT Rickettsia prowazekii RP600, hypothetical protein, FT TR:Q9ZCV8 (EMBL:AJ235272) (123 aa); Fasta score E(): FT 8.6e-05, 35.1% identity in 134 aa overlap. Previously FT sequenced as TR:O69593 (EMBL:AL023514) (147 aa); Fasta FT score E(): 0, 100.0% identity in 147 aa overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv0390" FT CDS 360202..360638 FT /class="IV.C.2" FT /colour=0 FT /gene="ML0277" FT /note="Possible pseudogene similar to M. tuberculosis FT PPE-family proteins (Best blastx score 74)" FT /product="PPE-family protein (pseudogene)" FT /pseudo FT CDS 360751..361125 FT /class="V" FT /colour=0 FT /gene="ML0278" FT /note="Possible pseudogene similar to M. tuberculosis FT paralogue Rv0387c (Best blastx score 97)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(361297..361953) FT /class="V" FT /colour=10 FT /gene="ML0279" FT /note="Similar to M. tuberculosis Rv0356c, hypothetical FT protein, TR:O06307 (EMBL:AL123456) (214 aa); Fasta score FT E(): 0, 73.4% identity in 214 aa overlap. Previously FT sequenced as TR:O69594 (EMBL:AL023514) (218 aa); Fasta FT score E(): 0, 99.5% identity in 218 aa overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv0356c" FT CDS complement(361950..363248) FT /EC_number="6.3.4.4" FT /class="I.F.1" FT /colour=7 FT /gene="purA" FT /gene="ML0280" FT /note="Similar to M. tuberculosis purA, Rv0357c, FT adenylosuccinate synthase, SW:PURA_MYCTU (O08381) (432 FT aa); Fasta score E(): 0, 87.9% identity in 431 aa overlap. FT Similar to many e.g. Escherichia coli purA, FT adenylosuccinate synthase, SW:PURA_ECOLI (P12283) (431 FT aa); Fasta score E(): 0, 51.1% identity in 425 aa overlap. FT Previously sequenced as SW:PURA_MYCLE (O69595) (432 aa); FT Fasta score E(): 0, 100.0% identity in 432 aa overlap. FT Contains Pfam match to entry PF00709 Adenylsucc_synt, FT Adenylosuccinate synthetase. Contains PS00513 FT Adenylosuccinate synthetase active site. Contains PS01266 FT Adenylosuccinate synthetase GTP-binding site." FT /product="putative adenylosuccinate synthase" FT /tb_orthologue="purA" FT RBS complement(361960..361964) FT /note="possible RBS" FT misc_feature complement(362070..363236) FT /colour=0 FT /note="Pfam match to entry PF00709 Adenylsucc_synt, FT Adenylosuccinate synthetase, score 729.00, E-value FT 2.1e-215" FT misc_feature complement(362820..362855) FT /colour=8 FT /note="PS00513 Adenylosuccinate synthetase active site" FT misc_feature complement(363198..363221) FT /colour=8 FT /note="PS01266 Adenylosuccinate synthetase GTP-binding FT site" FT RBS 363423..363427 FT /note="possible RBS" FT CDS 363432..364121 FT /class="V" FT /colour=10 FT /gene="ML0281" FT /note="Similar to M. tuberculosis Rv0358, hypothetical FT protein, TR:O06308 (EMBL:AL123456) (215 aa); Fasta score FT E(): 0, 62.4% identity in 229 aa overlap. Previously FT sequenced as TR:O69596 (EMBL:AL023514) (229 aa); Fasta FT score E(): 0, 99.6% identity in 229 aa overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv0358" FT CDS 364131..364909 FT /class="V" FT /colour=0 FT /gene="ML0282" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv0359 (Best blastx score 161)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS 364952..365722 FT /class="III.A.2" FT /colour=3 FT /gene="ML0283" FT /ml_unique FT /note="Similar to many cation-efflux transporter FT components e.g. Alcaligenes eutrophus czcD, FT cobalt-zinc-cadmium resistance protein, SW:CZCD_ALCEU FT (P13512) (316 aa); Fasta score E(): 3.8e-22, 36.4% FT identity in 209 aa overlap. Previously sequenced as FT TR:O69597 (EMBL:AL023514) (256 aa); Fasta score E(): 0, FT 100.0% identity in 256 aa overlap. Contains hydrophobic, FT possible membrane-spanning regions. Beyond codon 220 FT (approx.) GC frameplot and codon useage plots indicate a FT possible frame change. Similarity to characterised FT transporter components ends at this point. This suggests FT that the ORF may have suffered a disruption and therefore FT not encode a functional product. Contains Pfam match to FT entry PF01545 Cation_efflux, Cation efflux family." FT /product="putative cation-efflux transporter component" FT misc_feature 365174..365719 FT /colour=0 FT /note="Pfam match to entry PF01545 Cation_efflux, Cation FT efflux family, score 17.10, E-value 0.0001" FT CDS complement(365860..366273) FT /class="V" FT /colour=10 FT /gene="ML0284" FT /note="Similar to M. tuberculosis Rv0360c, hypothetical FT protein, TR:O06310 (EMBL:AL123456) (145 aa); Fasta score FT E(): 0, 85.4% identity in 137 aa overlap and Streptomyces FT coelicolor SCH10.25C, hypothetical protein, TR:Q9X8R4 FT (EMBL:AL049754) (143 aa); Fasta score E(): 8.2e-27, 56.2% FT identity in 130 aa overlap. Previously sequenced as FT TR:O69598 (EMBL:AL023514) (137 aa); Fasta score E(): 0, FT 100.0% identity in 137 aa overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv0360c" FT RBS complement(366277..366283) FT /note="possible RBS" FT RBS 366374..366378 FT /note="possible RBS" FT CDS 366385..367263 FT /class="II.C.4" FT /colour=3 FT /gene="ML0285" FT /note="Similar to M. tuberculosis Rv0361, possible FT membrane protein, TR:O06311 (EMBL:AL123456) (275 aa); FT Fasta score E(): 0, 58.6% identity in 292 aa overlap. FT Previously sequenced as TR:O69599 (EMBL:AL023514) (292 FT aa); Fasta score E(): 0, 100.0% identity in 292 aa FT overlap. Contains hydrophobic, possible membrane-spanning FT region." FT /product="putative membrane protein" FT CDS complement(367279..368316) FT /EC_number="4.1.2.13" FT /class="I.B.1" FT /colour=7 FT /gene="fba" FT /gene="ML0286" FT /note="Similar to M. tuberculosis fba, Rv0363c, fructose FT bisphosphate aldolase, SW:ALF_MYCTU (O06313) (344 aa); FT Fasta score E(): 0, 87.7% identity in 342 aa overlap. FT Similar to many e.g. Corynebacterium glutamicum, fructose FT bisphosphate aldolase, SW:ALF_CORGL (P19537) (343 aa); FT Fasta score E(): 0, 72.8% identity in 342 aa overlap. FT Previously sequenced as SW:ALF_MYCLE (O69600) (345 aa); FT Fasta score E(): 0, 100.0% identity in 345 aa overlap. FT Contains Pfam match to entry PF01116 F_bP_aldolase, FT Fructose-bisphosphate aldolase class-II. Contains PS00806 FT Fructose-bisphosphate aldolase class-II signature 2. FT Contains PS00602 Fructose-bisphosphate aldolase class-II FT signature 1." FT /product="putative fructose bisphosphate aldolase" FT /tb_orthologue="fba" FT misc_feature complement(367294..368304) FT /colour=0 FT /note="Pfam match to entry PF01116 F_bP_aldolase, FT Fructose-bisphosphate aldolase class-II, score 484.30, FT E-value 9.4e-142" FT misc_feature complement(367810..367845) FT /colour=8 FT /note="PS00806 Fructose-bisphosphate aldolase class-II FT signature 2" FT misc_feature complement(368026..368061) FT /colour=8 FT /note="PS00602 Fructose-bisphosphate aldolase class-II FT signature 1" FT RBS complement(368324..368330) FT /note="possible RBS" FT CDS 368449..369117 FT /class="II.C.4" FT /colour=3 FT /gene="ML0287" FT /note="Similar to M. tuberculosis Rv0364, hypothetical FT protein, SW:Y364_MYCTU (O06314) (227 aa); Fasta score E(): FT 0, 66.1% identity in 227 aa overlap. Similar to many FT dedA-family proteins e.g. Streptomyces coelicolor FT SCE9.18C, possible membrane protein, TR:Q9X8J1 FT (EMBL:AL049841) (303 aa); Fasta score E(): 0, 42.8% FT identity in 201 aa overlap. Previously sequenced as FT TR:O69601 (EMBL:AL023514) (222 aa); Fasta score E(): 0, FT 100.0% identity in 222 aa overlap. Contains hydrophobic, FT possible membrane-spanning regions. Contains Pfam match to FT entry PF00597 DedA, DedA family." FT /product="putative membrane protein" FT /tb_orthologue="Rv0364" FT misc_feature 368536..369018 FT /colour=0 FT /note="Pfam match to entry PF00597 DedA, DedA family, FT score 99.60, E-value 6e-26" FT repeat_region 369095..371590 FT CDS 369473..369612 FT /class="IV.B.1.c" FT /colour=0 FT /gene="ML0288" FT /note="Similar to Pseudomonas putida transposase tnpa1 FT TR:Q9R9U9 (EMBL:AJ245436) (355 aa) fasta scores: E(): FT 3.4e-05, 47.8% id in 46 aa, and to Agrobacterium FT tumefaciens transposase TR:Q44454 (EMBL:Z18270) (366 aa) FT fasta scores: E(): 0.00026, 41.3% id in 46 aa" FT /pseudo FT /product="transposase (pseudogene)" FT CDS 369817..370960 FT /class="IV.B" FT /colour=0 FT /gene="ML0289" FT /note="Similar to many e.g. Cryphonectria parasitica FT (Chesnut blight fungus) putative maturase TR:AAF27656 FT (EMBL:AF218567) (778 aa) fasta scores: E(): 7.8e-11, 29.2% FT id in 216 aa" FT /product="putative group II intron maturase-related FT protein" FT /pseudo FT stem_loop 370444..370497 FT CDS 371225..371323 FT /class="IV.B.1.c" FT /colour=0 FT /gene="ML0290" FT /note="Similar to Agrobacterium tumefaciens transposase FT TR:Q44454 (EMBL:Z18270) (366 aa) fasta scores: E(): FT 0.0002, 51.5% id in 33 aa, and to Pseudomonas putida FT transposase tnpa1 TR:Q9R9U9 (EMBL:AJ245436) (355 aa) fasta FT scores: E(): 0.00088, 48.5% id in 33 aa" FT /pseudo FT /product="transposase (pseudogene)" FT CDS complement(372034..372426) FT /class="VI" FT /colour=8 FT /gene="ML0291" FT /ml_unique FT /note="Unknown function. Previously sequenced as TR:O69603 FT (EMBL:AL023514) (130 aa); Fasta score E(): 0, 99.2% FT identity in 130 aa overlap." FT /product="hypothetical protein" FT CDS complement(373605..373844) FT /class="VI" FT /colour=8 FT /gene="ML0292" FT /ml_unique FT /note="Unknown function. Possibly the remains of a FT disrupted gene." FT /product="hypothetical protein" FT RBS complement(373854..373857) FT /note="possible RBS" FT CDS complement(375453..375662) FT /class="VI" FT /colour=8 FT /gene="ML0293" FT /ml_unique FT /note="Unknown function. Possibly the remains of a FT disrupted gene." FT /product="hypothetical protein" FT RBS 377474..377479 FT /note="possible RBS" FT CDS 377487..379130 FT /class="I.G.7" FT /colour=7 FT /gene="thiC" FT /gene="ML0294" FT /note="Similar to M. tuberculosis thiC, Rv0423c, thiamine FT biosynthesis protein, SW:THIC_MYCTU (P96269) (547 aa); FT Fasta score E(): 0, 90.1% identity in 547 aa overlap. FT Similar to many e.g. Escherichia coli thiC, thiamine FT biosynthesis protein, SW:THIC_ECOLI (P30136) (631 aa); FT Fasta score E(): 0, 59.2% identity in 593 aa overlap. FT Previously sequenced as SW:THIC_MYCLE (Q9ZBL0) (547 aa); FT Fasta score E(): 0, 100.0% identity in 547 aa overlap. FT Contains Pfam match to entry PF01964 ThiC, ThiC family." FT /product="putative thiamine biosynthesis protein" FT /tb_orthologue="thiC" FT misc_feature 377742..379013 FT /colour=0 FT /note="Pfam match to entry PF01964 ThiC, ThiC family, FT score 1105.40, E-value 0" FT CDS 379127..379966 FT /EC_number="2.7.4.7" FT /class="I.G.8" FT /colour=7 FT /gene="thiD" FT /gene="ML0295" FT /note="Similar to M. tuberculosis thiD, Rv0422c, FT phosphomethylpyrimidine kinase, SW:THID_MYCTU (P96268) FT (265 aa); Fasta score E(): 0, 77.8% identity in 266 aa FT overlap. Similar to many e.g. Escherichia coli thiD, FT phosphomethylpyrimidine kinase, SW:THID_ECOLI (P76422) FT (266 aa); Fasta score E(): 3.2e-29, 38.4% identity in 263 FT aa overlap. Previously sequenced as SW:THID_MYCLE (Q9ZBL1) FT (279 aa); Fasta score E(): 0, 99.6% identity in 279 aa FT overlap." FT /product="putative phosphomethylpyrimidine kinase" FT /tb_orthologue="thiD" FT CDS 380011..380147 FT /class="V" FT /colour=0 FT /gene="ML0296" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv0421c (Best blastx score 85)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(380645..381430) FT /class="I.G.8" FT /colour=7 FT /gene="thiG" FT /gene="ML0297" FT /note="Similar to M. tuberculosis Rv0417, hypothetical FT protein, TR:P96263 (EMBL:AL123456) (252 aa); Fasta score FT E(): 0, 86.8% identity in 250 aa overlap. Similar to many FT proteins involved in the biosynthesis of the thiazole FT moiety of thiamine e.g. Escherichia coli thiG, thiamine FT biosynthetic protein (thiazole moiety), SW:THIG_ECOLI FT (P30139) (281 aa); Fasta score E(): 0, 51.2% identity in FT 244 aa overlap. Previously sequenced as TR:Q9ZBL2 FT (EMBL:AL035159) (261 aa); Fasta score E(): 0, 99.6% FT identity in 261 aa overlap." FT /product="putative thiamine biosynthesis protein" FT CDS complement(381423..381647) FT /class="V" FT /colour=10 FT /gene="ML0298" FT /note="Similar to M. tuberculosis Rv0416, conserved FT hypothetical protein, TR:P96262 (EMBL:AL123456) (68 aa); FT Fasta score E(): 5.9e-17, 71.6% identity in 74 aa overlap. FT Similar to bacterial hypothetical protein e.g. Escherichia FT coli thiS, hypothetical protein, SW:THIS_ECOLI (O32583) FT (66 aa); Fasta score E(): 0.41, 32.0% identity in 50 aa FT overlap. Previously sequenced as TR:Q9ZBL3 (EMBL:AL035159) FT (74 aa); Fasta score E(): 1.2e-28, 100.0% identity in 74 FT aa overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv0416" FT CDS complement(381655..382677) FT /class="I.B.7" FT /colour=7 FT /gene="ML0299" FT /note="Similar to M. tuberculosis Rv0415, conserved FT hypothetical protein, TR:P96261 (EMBL:AL123456) (340 aa); FT Fasta score E(): 0, 82.0% identity in 338 aa overlap. FT Shows weak similarity to Bacillus subtilis goxB, glycine FT oxidase, SW:GLOX_BACSU (O31616) (369 aa); Fasta score E(): FT 6.6e-10, 28.7% identity in 352 aa overlap. Previously FT sequenced as TR:Q9ZBL4 (EMBL:AL035159) (340 aa); Fasta FT score E(): 0, 100.0% identity in 340 aa overlap. Contains FT Pfam match to entry PF01266 DAO, D-amino acid oxidase." FT /note="Shows weak similarity to ML2011" FT /product="putative oxidoreductase" FT /tb_orthologue="Rv0415" FT misc_feature complement(381664..382656) FT /colour=0 FT /note="Pfam match to entry PF01266 DAO, D-amino acid FT oxidase, score 35.40, E-value 3.5e-09" FT RBS complement(382683..382688) FT /note="possible RBS" FT CDS 382835..383542 FT /EC_number="2.5.1.3" FT /class="I.G.8" FT /colour=7 FT /gene="thiE" FT /gene="ML0300" FT /note="Similar to M. tuberculosis thiE, Rv0414c, putative FT thiamine-phosphate pyrophosphorylase, SW:THIE_MYCTU FT (P96260) (222 aa); Fasta score E(): 0, 78.9% identity in FT 223 aa overlap. Similar to many e.g. Escherichia coli FT thiE, thiamine-phosphate pyrophosphorylase, SW:THIE_ECOLI FT (P30137) (211 aa); Fasta score E(): 5e-10, 38.7% identity FT in 199 aa overlap. Previously sequenced as SW:THIE_MYCLE FT (Q9ZBL5) (235 aa); Fasta score E(): 0, 99.6% identity in FT 235 aa overlap." FT /product="putative thiamine-phosphate pyrophosphorylase" FT /tb_orthologue="thiE" FT CDS complement(383529..384154) FT /class="II.A.5" FT /colour=0 FT /gene="mutT3" FT /gene="ML0301" FT /note="Possible pseudogene of M. tuberculosis orthologue FT mutT3 (Best blastx score 180)" FT /product="MutT homologue (pseudogene)" FT /pseudo FT CDS 384334..385649 FT /class="II.C.4" FT /colour=0 FT /gene="ML0302" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv0412c (Best blastx score 1672)" FT /product="putative membrane protein (pseudogene)" FT /pseudo FT RBS 385638..385642 FT /note="possible RBS" FT CDS 385649..386629 FT /class="III.A.1" FT /colour=3 FT /gene="glnH" FT /gene="ML0303" FT /note="Similar to M. tuberculosis glnH, Rv0411c, putative FT glutamine-binding protein, TR:P96257 (EMBL:AL123456) (328 FT aa); Fasta score E(): 0, 79.3% identity in 328 aa overlap. FT Similar to many bacterial solute-binding proteins e.g. FT Bacillus stearothermophilus glnH, glutamine-binding FT protein, SW:GLNH_BACST (P27676) (262 aa); Fasta score E(): FT 3.7e-23, 38.1% identity in 194 aa overlap. Previously FT sequenced as TR:Q9ZBL7 (EMBL:AL035159) (325 aa); Fasta FT score E(): 0, 100.0% identity in 325 aa overlap. Contains FT a probable N-terminal signal sequence. Contains Pfam match FT to entry PF00497 SBP_bac_3, Bacterial extracellular FT solute-binding proteins, family 3. Contains PS01039 FT Bacterial extracellular solute-binding proteins, family 3 FT signature. Contains PS00013 Prokaryotic membrane FT lipoprotein lipid attachment site." FT /product="putative glutamine-binding protein" FT /tb_orthologue="glnH" FT misc_feature 385670..386578 FT /colour=0 FT /note="Pfam match to entry PF00497 SBP_bac_3, Bacterial FT extracellular solute-binding proteins, family 3, score FT 98.70, E-value 1.2e-25" FT misc_feature 385688..385720 FT /colour=8 FT /note="PS00013 Prokaryotic membrane lipoprotein lipid FT attachment site" FT misc_feature 385991..386032 FT /colour=8 FT /note="PS01039 Bacterial extracellular solute-binding FT proteins, family 3 signature" FT CDS 386629..388932 FT /class="I.J.3" FT /colour=9 FT /gene="pknG" FT /gene="ML0304" FT /note="Similar to M. tuberculosis pknG, Rv0410c, putative FT serine/threonine protein kinase, TR:P96256 (EMBL:AL123456) FT (750 aa); Fasta score E(): 0, 83.1% identity in 756 aa FT overlap. Similar to many bacterial putative FT serine/threonine protein kinases e.g. Streptomyces FT coelicolor SC6D10.09, possible protein kinase, TR:CAB71204 FT (EMBL:AL138538) (903 aa); Fasta score E(): 0, 42.0% FT identity in 793 aa overlap. Similar in part, to protein FT kinases from Myxococcus xanthus e.g. pkn12, FT serine/threonine protein kinase, TR:Q9XBP5 (EMBL:AF159692) FT (465 aa); Fasta score E(): 2.9e-10, 28.4% identity in 292 FT aa overlap. Previously sequenced as TR:Q9ZBL8 FT (EMBL:AL035159) (763 aa); Fasta score E(): 0, 100.0% FT identity in 763 aa overlap. Contains Pfam match to entry FT PF00069 pkinase, Eukaryotic protein kinase domain. FT Contains PS00108 Serine/Threonine protein kinases FT active-site signature." FT /note="Similar to regions of ML0016 and ML0017" FT /product="putative serine-threonine protein kinase" FT /tb_orthologue="pknG" FT misc_feature 387118..387693 FT /colour=0 FT /note="Pfam match to entry PF00069 pkinase, Eukaryotic FT protein kinase domain, score 91.00, E-value 3.9e-24" FT misc_feature 387493..387531 FT /colour=8 FT /note="PS00108 Serine/Threonine protein kinases FT active-site signature" FT repeat_region 389159..390696 FT CDS complement(390457..391901) FT /class="II.C.4" FT /colour=0 FT /gene="ML0305" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv0537c (Best blastx score 503)" FT /product="unknown, possible membrane protein, FT (pseudogene)" FT /pseudo FT CDS 392396..393159 FT /class="II.C.4" FT /colour=0 FT /gene="ML0306" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv0538 (Best blastx score 184)" FT /product="possible transmembrane protein (pseudogene)" FT /pseudo FT CDS 393500..393920 FT /class="V" FT /colour=0 FT /gene="ML0307" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv0434 (Best blastx score 130)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS 394457..395161 FT /class="V" FT /colour=10 FT /gene="ML0308" FT /ml_unique FT /note="Similar to hypothetical proteins from FT methanobacteria e.g. Methanobacterium thermoautotrophicum FT MTH1801, hypothetical protein, TR:O27829 (EMBL:AE000934) FT (224 aa); Fasta score E(): 3.6e-24, 39.3% identity in 219 FT aa overlap and Methanococcus jannaschii MJ0045, FT hypothetical protein, SW:Y045_METJA (Q60353) (221 aa); FT Fasta score E(): 4e-17, 30.5% identity in 220 aa overlap. FT Previously sequenced as TR:Q9ZBM1 (EMBL:AL035159) (234 FT aa); Fasta score E(): 0, 100.0% identity in 234 aa FT overlap." FT /product="conserved hypothetical protein" FT CDS complement(395181..397022) FT /class="III.C" FT /colour=0 FT /gene="ML0309" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv0435c (Best blastx score 329)" FT /product="ATPase of AAA-family (pseudogene)" FT /pseudo FT CDS complement(397046..397948) FT /EC_number="2.7.8.8" FT /class="I.H.3" FT /colour=1 FT /gene="pssA" FT /gene="ML0310" FT /note="Similar to M. tuberculosis pssA, Rv0436c, FT CDP-diacylglycerol-serine o-phosphatidyltransferase, FT SW:PSS_MYCTU (P96282) (286 aa); Fasta score E(): 0, 77.9% FT identity in 285 aa overlap. Similar to many e.g. FT Helicobacter pylori pssA, CDP-diacylglycerol-serine FT o-phosphatidyltransferase, SW:PSS_HELPY (Q48269) (237 aa); FT Fasta score E(): 2.3e-14, 34.6% identity in 234 aa FT overlap. Previously sequenced as TR:Q9ZBM2 (EMBL:AL035159) FT (300 aa); Fasta score E(): 0, 100.0% identity in 300 aa FT overlap. Contains Pfam match to entry PF01066 FT CDP-OH_P_transf, CDP-alcohol phosphatidyltransferase. FT Contains PS00379 CDP-alcohol phosphatidyltransferases FT signature." FT /product="putative CDP-diacylglycerol-serine FT o-phosphatidyltransferase" FT /tb_orthologue="pssA" FT misc_feature complement(397271..397819) FT /colour=0 FT /note="Pfam match to entry PF01066 CDP-OH_P_transf, FT CDP-alcohol phosphatidyltransferase, score -1.50, E-value FT 0.0012" FT misc_feature complement(397712..397780) FT /colour=8 FT /note="PS00379 CDP-alcohol phosphatidyltransferases FT signature" FT CDS complement(397945..398676) FT /class="V" FT /colour=10 FT /gene="ML0311" FT /note="Similar to M. tuberculosis psd, Rv0437c, putative FT phosphatidylserine decarboxylase, TR:O86324 FT (EMBL:AL123456) (231 aa); Fasta score E(): 0, 72.6% FT identity in 241 aa overlap. Similar to many bacterial FT hypothetical proteins e.g. Rickettsia prowazekii RP241, FT hypothetical protein, TR:Q9ZDT4 (EMBL:AJ235271) (231 aa); FT Fasta score E(): 5.2e-31, 43.3% identity in 215 aa overlap FT and Neisseria meningitidis MC58 NMB0963, FT phosphatidylserine decarboxylase precursor-related FT protein, TR:AAF41369 (EMBL:AE002447) (265 aa); Fasta score FT E(): 2.5e-23, 39.2% identity in 217 aa overlap. Previously FT sequenced as TR:Q9ZBM3 (EMBL:AL035159) (202 aa); Fasta FT score E(): 0, 99.5% identity in 202 aa overlap. The start FT codon is uncertain. Codon usage suggests an alternative FT start at codon 63 (approx)." FT /product="conserved hypothetical protein" FT /tb_orthologue="psd" FT CDS complement(398720..399953) FT /class="I.G.4" FT /colour=0 FT /gene="moaA3" FT /gene="ML0312" FT /note="Possible pseudogene of M. tuberculosis orthologue FT moaA3 (Best blastx score 245)" FT /product="molybdenum cofactor biosynthesis, protein A FT (pseudogene)" FT /pseudo FT CDS complement(400042..400675) FT /class="I.B.7" FT /colour=0 FT /gene="ML0313" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv0439c (Best blastx score 248)" FT /product="probable oxidoreductase (pseudogene)" FT /pseudo FT CDS complement(400771..401778) FT /class="II.B.5" FT /colour=7 FT /gene="ML0314" FT /note="Similar to M. tuberculosis lipU, Rv1076, probable FT esterase, TR:O53424 (EMBL:AL123456) (297 aa); Fasta score FT E(): 0, 79.7% identity in 296 aa overlap. Also similar to FT other putative esterases from M. tuberculosis. Similar to FT esterases e.g. Streptomyces hygroscopicus bah, FT acetyl-hydrolase, SW:BAH_STRHY (Q01109) (299 aa); Fasta FT score E(): 6.6e-19, 36.2% identity in 246 aa overlap. FT Previuosly sequenced as TR:Q9ZBM4 (EMBL:AL035159) (335 FT aa); Fasta score E(): 0, 99.7% identity in 335 aa overlap. FT Contains PS01174 Lipolytic enzymes 'G-D-X-G' family, FT putative serine active site." FT /product="putative esterase" FT /tb_orthologue="lipU" FT misc_feature complement(401257..401295) FT /colour=8 FT /note="PS01174 Lipolytic enzymes 'G-D-X-G' family, FT putative serine active site" FT CDS complement(403698..404612) FT /class="I.B.7" FT /colour=7 FT /gene="ML0315" FT /note="Similar to M. tuberculosis Rv0068, putative FT oxidoreductase, TR:O53613 (EMBL:AL123456) (303 aa); Fasta FT score E(): 0, 73.9% identity in 306 aa overlap and to FT other M. tuberculosis putative oxidoreductases (e.g. FT Rv0439c). Similar to many bacterial putative FT oxidoreductases e.g. Streptomyces coelicolor SC7A8.30C, FT putative oxidoreductase, TR:CAB69779 (EMBL:AL137187) (310 FT aa); Fasta score E(): 0, 47.9% identity in 311 aa overlap. FT Previously sequenced as TR:Q9ZBM5 (EMBL:AL035159) (304 FT aa); Fasta score E(): 0, 100.0% identity in 304 aa FT overlap. Contains Pfam match to entry PF00106 adh_short, FT short chain dehydrogenase." FT /product="putative oxidoreductase" FT /tb_orthologue="Rv0068" FT misc_feature complement(403974..404570) FT /colour=0 FT /note="Pfam match to entry PF00106 adh_short, short chain FT dehydrogenase, score 95.90, E-value 7.9e-25" FT RBS complement(404619..404623) FT /note="possible RBS" FT CDS 404707..405309 FT /class="I.J.1" FT /colour=9 FT /gene="ML0316" FT /note="Similar to M. tuberculosis Rv0067c, putative FT transcriptional regulator, TR:O53612 (EMBL:AL123456) (189 FT aa); Fasta score E(): 0, 68.8% identity in 189 aa overlap. FT Similar to many putative transcriptional regulators e.g. FT Streptomyces coelicolor SCF6.16, putative tetR family FT transcriptional regulator, TR:Q9RJL5 (EMBL:AL121849) (194 FT aa); Fasta score E(): 2.5e-18, 39.5% identity in 185 aa FT overlap. Previously sequenced as TR:Q9ZBM6 (EMBL:AL035159) FT (189 aa); Fasta score E(): 0, 100.0% identity in 189 aa FT overlap. Contains a probable helix-turn-helix motif at aa FT 45-66 (Score 1800, SD +5.32) Contains Pfam match to entry FT PF00440 tetR, Bacterial regulatory proteins, tetR family." FT /product="putative TetR/AcrR-family transcriptional FT regulator" FT /tb_orthologue="Rv0067c" FT misc_feature 404794..404931 FT /colour=0 FT /note="Pfam match to entry PF00440 tetR, Bacterial FT regulatory proteins, tetR family, score 62.00, E-value FT 1.3e-14" FT RBS 405501..405505 FT /note="possible RBS" FT CDS 405518..407143 FT /class="III.B" FT /colour=0 FT /gene="groEL2" FT /gene="ML0317" FT /note="Similar to M. tuberculosis groEL2, Rv0440, 60 kDa FT chaperonin 2, SW:CH62_MYCTU (P06806) (539 aa); Fasta score FT E(): 0, 94.8% identity in 541 aa overlap. A major FT immunoreactive protein (65 kDa antigen). Previously FT sequenced as SW:CH62_MYCLE (P09239) (540 aa); Fasta score FT E(): 0, 100.0% identity in 540 aa overlap. Contains Pfam FT match to entry PF00118 cpn60_TCP1, TCP-1/cpn60 chaperonin FT family. Contains PS00296 Chaperonins cpn60 signature." FT /note="Similar to ML0381" FT /product="60 kDa chaperonin 2" FT /tb_orthologue="groEL2" FT misc_feature 405581..407083 FT /colour=0 FT /note="Pfam match to entry PF00118 cpn60_TCP1, TCP-1/cpn60 FT chaperonin family, score 777.80, E-value 4.3e-230" FT misc_feature 406724..406759 FT /colour=8 FT /note="PS00296 Chaperonins cpn60 signature" FT repeat_region 407294..408407 FT CDS complement(408187..409345) FT /class="II.A.5" FT /colour=0 FT /gene="radA" FT /gene="ML0318" FT /note="Possible pseudogene of M. tuberculosis orthologue FT radA (Best blastx score 221)" FT /product="probable DNA repair RadA homologue (pseudogene)" FT /pseudo FT CDS complement(409442..409993) FT /class="II.C.1" FT /colour=3 FT /gene="lpqE" FT /gene="ML0319" FT /note="Similar to M. tuberculosis lpqE, Rv3584, putative FT lipoprotein, TR:O53569 (EMBL:AL123456) (182 aa); Fasta FT score E(): 0, 63.4% identity in 175 aa overlap. Previously FT sequenced as TR:Q9ZBM7 (EMBL:AL035159) (183 aa); Fasta FT score E(): 0, 99.5% identity in 183 aa overlap. Contains a FT probable N-terminal signal sequnce. Contains PS00013 FT Prokaryotic membrane lipoprotein lipid attachment site." FT /product="putative lipoprotein" FT /tb_orthologue="lpqE" FT misc_feature complement(409901..409933) FT /colour=8 FT /note="PS00013 Prokaryotic membrane lipoprotein lipid FT attachment site" FT RBS complement(410000..410005) FT /note="possible RBS" FT CDS 410354..410851 FT /class="I.J.1" FT /colour=9 FT /gene="ML0320" FT /note="Similar to M. tuberculosis Rv3583c, putative FT transcription factor, TR:O53568 (EMBL:AL123456) (162 aa); FT Fasta score E(): 0, 97.5% identity in 162 aa overlap. FT Shows weak similarity to Myxococcus xanthus carD, FT transcription factor, TR:Q50887 (EMBL:Z56280) (316 aa); FT Fasta score E(): 9.1e-13, 31.2% identity in 154 aa overlap FT and to many hypothetical transcription factors." FT /product="putative transcription factor" FT /tb_orthologue="Rv3583c" FT RBS 410859..410862 FT /note="possible RBS" FT CDS 410870..411595 FT /class="I.C.1" FT /colour=7 FT /gene="ML0321" FT /note="Similar to M. tuberculosis Rv3582c, conserved FT hypothetical protein, SW:YZ82_MYCTU (P96864) (231 aa); FT Fasta score E(): 0, 66.0% identity in 241 aa overlap. FT Similar to many bacterial hypothetical proteins e.g. FT Streptomyces coelicolor SCD8A.06, hypothetical protein, FT TR:CAB77327 (EMBL:AL160331) (270 aa); Fasta score E(): FT 4.3e-25, 45.0% identity in 240 aa overlap. Shows weak FT similarity to non-mevalonate pathway enzyme FT 4-diphosphocytidyl-2C-methyl-D-erythritol synthases e.g. FT Escherichia coli ispD, FT 4-diphosphocytidyl-2C-methyl-D-erythritol synthase, FT TR:AAF43207 (EMBL:AF230736) (236 aa); Fasta score E(): FT 0.0016, 36.1% identity in 244 aa overlap. Contains Pfam FT match to entry PF01128 UPF0007, Uncharacterized protein FT family UPF0007." FT /product="putative FT 4-diphosphocytidyl-2C-methyl-D-erythritol synthase" FT /tb_orthologue="Rv3582c" FT misc_feature 410891..411592 FT /colour=0 FT /note="Pfam match to entry PF01128 UPF0007, FT Uncharacterized protein family UPF0007, score 293.70, FT E-value 2.3e-84" FT CDS 411592..412068 FT /class="I.C.1" FT /colour=7 FT /gene="ML0322" FT /note="Similar to M. tuberculosis Rv3581c, hypothetical FT protein, SW:YZ81_MYCTU (P96863) (159 aa); Fasta score E(): FT 0, 79.1% identity in 158 aa overlap. Similar to FT 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthases, FT e.g. Escherichia coli 2-C-methyl-D-erythritol FT 2,4-cyclodiphosphate synthase, TR:BAA95145 (EMBL:AB038256) FT (159 aa); Fasta score E(): 1e-17, 41.3% identity in 155 aa FT overlap. Contains Pfam match to entry PF01128 UPF0007, FT Uncharacterized protein family UPF0007." FT /product="putative 2-C-methyl-D-erythritol FT 2,4-cyclodiphosphate synthase" FT /tb_orthologue="Rv3581c" FT CDS 412123..413544 FT /EC_number="6.1.1.16" FT /class="II.A.3" FT /colour=2 FT /gene="cysS" FT /gene="ML0323" FT /note="Similar to M. tuberculosis cysS, Rv3580c, FT cysteinyl-tRNA synthetase, SW:SYC1_MYCTU (P96862) (469 FT aa); Fasta score E(): 0, 86.5% identity in 467 aa overlap. FT Similar to many e.g. Escherichia coli cysS, cysteinyl-tRNA FT synthetase, SW:SYC_ECOLI (P21888) (461 aa); Fasta score FT E(): 0, 42.0% identity in 460 aa overlap. Contains Pfam FT match to entry PF01406 tRNA-synt_1e, tRNA synthetases FT class I (C)." FT /note="Similar to ML1302" FT /product="putative cysteinyl-tRNA synthase" FT /tb_orthologue="cysS" FT misc_feature 412177..413472 FT /colour=0 FT /note="Pfam match to entry PF01406 tRNA-synt_1e, tRNA FT synthetases class I (C), score 815.60, E-value 1.8e-241" FT CDS 413627..414463 FT /class="IV.H" FT /colour=7 FT /gene="ML0324" FT /note="Similar to M. tuberculosis Rv3579c, putative FT methyltransferase, TR:P96861 (EMBL:AL123456) (322 aa); FT Fasta score E(): 0, 84.1% identity in 277 aa overlap. FT Similar Streptomyces actuosus nhs, 23S rRNA FT methyltransferase and to many hypothetical rRNA FT methyltransferases. Contains Pfam match to entry PF00588 FT SpoU_methylase, SpoU rRNA Methylase family." FT /product="putative methyltransferase" FT /tb_orthologue="Rv3579c" FT misc_feature 414011..414436 FT /colour=0 FT /note="Pfam match to entry PF00588 SpoU_methylase, SpoU FT rRNA Methylase family, score 159.60, E-value 5.2e-44" FT RBS 414843..414848 FT /note="possible RBS" FT CDS 414865..415128 FT /class="VI" FT /colour=8 FT /gene="ML0325" FT /ml_unique FT /note="Unknown function. Possibly the remains of a FT disrupted gene." FT /product="hypothetical protein" FT CDS complement(416569..416845) FT /class="V" FT /colour=0 FT /gene="ML0326" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv0288 (Best blastx score 118)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(416866..417152) FT /class="V" FT /colour=0 FT /gene="ML0327" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv0287 (Best blastx score 124)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(417242..418418) FT /class="IV.C.2" FT /colour=0 FT /gene="PPE" FT /gene="ML0328" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv0286, PPE (Best blastx score 169)" FT /product="PPE-family protein (pseudogene)" FT /pseudo FT CDS complement(420549..420876) FT /class="V" FT /colour=0 FT /gene="ML0329" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1942c (Best blastx score 168)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(420876..420989) FT /class="V" FT /colour=0 FT /gene="ML0330" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1943c (Best blastx score 154)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(421105..422310) FT /class="III.A.4" FT /colour=0 FT /gene="arsB2" FT /gene="ML0331" FT /note="Possible pseudogene of M. tuberculosis orthologue FT arsB2 (Best blastx score 423)" FT /product="probable arsenical pump (pseudogene)" FT /pseudo FT CDS complement(422317..423055) FT /class="V" FT /colour=0 FT /gene="ML0332" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3577 (Best blastx score 257)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(423178..423936) FT /class="V" FT /colour=10 FT /gene="ML0333" FT /ml_unique FT /note="N-terminal half is similar to that of Emericella FT nidulans lamB, lactam utilization protein, SW:LAMB_EMENI FT (P38096) (262 aa); Fasta score E(): 2.6e-15, 38.3% FT identity in 128 aa overlap. Similar to many bacterial FT hypothetical proteins e.g. TR:Q9RL45 (EMBL:AL121596) (250 FT aa); Fasta score E(): 0, 58.1% identity in 248 aa FT overlap." FT /product="conserved hypothetical protein" FT CDS complement(424106..424678) FT /class="V" FT /colour=0 FT /gene="pknM" FT /gene="ML0334" FT /note="Possible pseudogene of M. tuberculosis orthologue FT pknM (Best blastx score 208)" FT /product="similar to ser-thr-protein kinases (pseudogene)" FT /pseudo FT CDS complement(424867..425727) FT /class="III.A" FT /colour=3 FT /gene="ML0335" FT /note="Similar in part to M. tuberculosis Rv2060, FT conserved hypothetical protein, TR:O86339 (EMBL:AL123456) FT (133 aa); Fasta score E(): 0, 80.3% identity in 132 aa FT overlap. Similar, except N-terminal approx. 100 aa to many FT bacterial ABC transporters e.g. Streptococcus pneumoniae FT adcB, putative ABC metal permease, TR:O33704 (EMBL:Z71552) FT (268 aa); Fasta score E(): 2.5e-15, 26.6% identity in 263 FT aa overlap. Contains hydrophobic, possible FT membrane-spanning regions. Contains Pfam match to entry FT PF00950 ABC-3, ABC 3 transport family." FT /product="putative ABC-transporter transmembrane protein" FT /tb_orthologue="Rv2060" FT misc_feature complement(424891..425661) FT /colour=0 FT /note="Pfam match to entry PF00950 ABC-3, ABC 3 transport FT family, score 95.40, E-value 1.1e-24" FT CDS complement(425727..426554) FT /class="III.A" FT /colour=3 FT /gene="ML0336" FT /ml_unique FT /note="Similar to many putative ABC-tranporter ATP-binding FT proteins e.g. Chlamydia trachomatis troB, ABC transporter FT ATPase, TR:O87477 (EMBL:AF077010) (286 aa); Fasta score FT E(): 4.7e-16, 31.4% identity in 223 aa overlap. Contains FT Pfam match to entry PF00005 ABC_tran, ABC transporter. FT Contains PS00017 ATP/GTP-binding site motif A (P-loop). FT Contains PS00211 ABC transporters family signature." FT /product="putative ABC-tranporter ATP-binding protein" FT misc_feature complement(425847..426413) FT /colour=0 FT /note="Pfam match to entry PF00005 ABC_tran, ABC FT transporter, score 148.40, E-value 1.2e-40" FT misc_feature complement(426033..426077) FT /colour=8 FT /note="PS00211 ABC transporters family signature" FT misc_feature complement(426369..426392) FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT CDS complement(426563..427471) FT /class="III.A" FT /colour=3 FT /gene="ML0337" FT /note="Shows weak similarity to periplasmic solute-binding FT proteins e.g. Synechocystis sp. (strain PCC 6803) mntC, FT periplasmic-binding protein, TR:Q55280 (EMBL:L34630) (330 FT aa); Fasta score E(): 0.0001, 26.4% identity in 178 aa FT overlap. Contains a possible N-terminal signal sequence. FT Contains Pfam match to entry PF01297 Lipoprotein_4, FT Adhesion lipoprotein. Contains PS00013 Prokaryotic FT membrane lipoprotein lipid attachment site." FT /product="putative periplasmic solute-binding proteins" FT misc_feature complement(426584..427450) FT /colour=0 FT /note="Pfam match to entry PF01297 Lipoprotein_4, Adhesion FT lipoprotein, score 2.70, E-value 4.5e-10" FT misc_feature complement(427412..427444) FT /colour=8 FT /note="PS00013 Prokaryotic membrane lipoprotein lipid FT attachment site" FT CDS 427651..428675 FT /class="I.J.1" FT /colour=0 FT /gene="ML0338" FT /note="Possible pseudogene similar to M. tuberculosis FT orthologue Rv3575c (Best blastx score 220)" FT /product="transcriptional regulator (LacI family) FT (pseudogene)" FT /pseudo FT CDS complement(428887..429249) FT /class="I.J.1" FT /colour=0 FT /gene="ML0339" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3574 (Best blastx score 269)" FT /product="transcriptional regulator (TetR/AcrR family) FT (pseudogene)" FT /pseudo FT CDS 429653..431495 FT /class="I.A.3" FT /colour=0 FT /gene="fadE34" FT /gene="ML0340" FT /note="Possible pseudogene of M. tuberculosis orthologue FT fadE34 (Best blastx score 539)" FT /product="acyl-CoA dehydrogenase (pseudogene)" FT /pseudo FT CDS complement(431727..432218) FT /class="V" FT /colour=0 FT /gene="ML0341" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3572 (Best blastx score 324)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(432323..433323) FT /class="I.B.7" FT /colour=0 FT /gene="ML0342" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3571 (Best blastx score 484)" FT /product="electron transfer component of (pseudogene)" FT /pseudo FT CDS 433560..434728 FT /class="I.B.7" FT /colour=0 FT /gene="ML0343" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3570c (Best blastx score 413)" FT /product="putative oxidoreductase (pseudogene)" FT /pseudo FT CDS 434746..435270 FT /class="IV.I" FT /colour=0 FT /gene="ML0344" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3569c (Best blastx score 339)" FT /product="probable hydrolase (pseudogene)" FT /pseudo FT CDS complement(435602..436741) FT /class="I.H.3" FT /colour=0 FT /gene="ML0345" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3523 (Best blastx score 319)" FT /product="lipid carrier protein (pseudogene)" FT /pseudo FT CDS complement(436783..437194) FT /class="I.J.1" FT /colour=0 FT /gene="ML0346" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3522 (Best blastx score 167)" FT /product="putative transcriptional regulator (pseudogene)" FT /pseudo FT CDS complement(437391..437981) FT /class="V" FT /colour=0 FT /gene="ML0347" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3521 (Best blastx score 236)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS 438262..439314 FT /class="IV.G" FT /colour=7 FT /gene="ML0348" FT /note="Similar to M. tuberculosis Rv3520c, possible FT coenzyme F420-dependent enzyme, TR:O53565 (EMBL:AL123456) FT (347 aa); Fasta score E(): 0, 86.8% identity in 342 aa FT overlap. Shows weak similarity to Methanobacterium FT thermoautotrophicum mer, methylenetetrahydromethanopterin FT reductase, TR:Q50744 (EMBL:X86477) (321 aa); Fasta score FT E(): 2.8e-05, 27.1% identity in 332 aa overlap." FT /product="possible coenzyme F420-dependent oxidoreductase" FT /tb_orthologue="Rv3520c" FT CDS complement(439393..439704) FT /class="V" FT /colour=0 FT /gene="ML0349" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3519 (Best blastx score 156)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(442415..443185) FT /class="IV.H" FT /colour=0 FT /gene="ML0350" FT /note="Possible pseudogene similar to M. tuberculosis FT paralogue Rv3729 (Best blastx score 294)" FT /product="probable transferase (pseudogene)" FT /pseudo FT CDS complement(444087..444737) FT /class="I.A.3" FT /colour=0 FT /gene="echA19" FT /gene="ML0351" FT /note="Possible pseudogene of M. tuberculosis orthologue FT echA19 (Best blastx score 238)" FT /product="enoyl-CoA hydratase/isomerase superfamily FT (pseudogene)" FT /pseudo FT CDS 444860..445795 FT /class="I.A.3" FT /colour=0 FT /gene="fadD19" FT /gene="ML0352" FT /note="Possible pseudogene of M. tuberculosis orthologue FT fadD19 (Best blastx score 221)" FT /product="acyl-CoA synthase (pseudogene)" FT /pseudo FT CDS 446609..447405 FT /class="V" FT /colour=0 FT /gene="ML0353" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3510c (Best blastx score 461)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT RBS 447402..447406 FT /note="possible RBS" FT CDS 447414..448961 FT /class="I.D.7" FT /colour=7 FT /gene="ilvX" FT /gene="ML0354" FT /note="Similar to M. tuberculosis ilvX, putative FT acetohydroxyacid synthase I large subunit, TR:O53554 FT (EMBL:AL123456) (515 aa); Fasta score E(): 0, 82.9% FT identity in 515 aa overlap. Similar to Escherichia coli FT ilvI, acetolactate synthase isozyme III large subunit, FT SW:ILVI_ECOLI (P00893) (574 aa); Fasta score E(): 1.8e-07, FT 23.7% identity in 540 aa overlap. Also similar to FT Pseudomonas putida mdlC, benzoylformate decarboxylase, FT SW:MDLC_PSEPU (P20906) (528 aa); Fasta score E(): 6.6e-13, FT 29.3% identity in 529 aa overlap. Previously sequenced as FT TR:Q49865 (EMBL:U00020) (515 aa); Fasta score E(): 0, FT 99.8% identity in 515 aa overlap. Contains 2 Pfam matches FT to entry PF00205 TPP_enzymes, Thiamine pyrophosphate FT enzymes." FT /product="putative acetohydroxyacid synthase I large FT subunit" FT /tb_orthologue="ilvX" FT misc_feature 447444..447710 FT /colour=0 FT /note="Pfam match to entry PF00205 TPP_enzymes, Thiamine FT pyrophosphate enzymes, score 47.60, E-value 3.5e-14" FT misc_feature 448551..448937 FT /colour=0 FT /note="Pfam match to entry PF00205 TPP_enzymes, Thiamine FT pyrophosphate enzymes, score 94.50, E-value 9.3e-29" FT CDS complement(449015..450183) FT /class="I.A.3" FT /colour=0 FT /gene="fadD17" FT /gene="ML0355" FT /note="Possible pseudogene of M. tuberculosis orthologue FT fadD17 (Best blastx score 515)" FT /product="acyl-CoA synthase (pseudogene)" FT /pseudo FT CDS complement(450363..451273) FT /class="I.A.3" FT /colour=0 FT /gene="fadE27" FT /gene="ML0356" FT /note="Possible pseudogene of M. tuberculosis orthologue FT fadE27 (Best blastx score 223)" FT /product="acyl-CoA dehydrogenase (pseudogene)" FT /pseudo FT CDS complement(451302..452424) FT /class="I.A.3" FT /colour=0 FT /gene="fadE26" FT /gene="ML0357" FT /note="Possible pseudogene of M. tuberculosis orthologue FT fadE26 (Best blastx score 551)" FT /product="acyl-CoA dehydrogenase (pseudogene)" FT /pseudo FT CDS 452646..452816 FT /class="I.B.6.c" FT /colour=0 FT /gene="ML0358" FT /note="Possible pseudogene of M. tuberculosis orthologue FT fdxD (Best blastx score 212)" FT /product="putative ferredoxin (pseudogene)" FT /pseudo FT CDS 452939..453841 FT /class="I.B.7" FT /colour=0 FT /gene="ML0359" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3502c (Best blastx score 300)" FT /product="putative dehydrogenase (pseudogene)" FT /pseudo FT CDS 454075..454785 FT /class="IV.A" FT /colour=0 FT /gene="ML0360" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3501c (Best blastx score 493)" FT /product="part of mce4 operon (pseudogene)" FT /pseudo FT CDS 454840..455649 FT /class="IV.A" FT /colour=0 FT /gene="ML0361" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3500c (Best blastx score 393)" FT /product="part of mce4 operon (pseudogene)" FT /pseudo FT CDS 455770..456328 FT /class="IV.A" FT /colour=0 FT /gene="mce4" FT /gene="ML0362" FT /note="Possible pseudogene of M. tuberculosis orthologue FT mce4 (Best blastx score 195)" FT /product="cell invasion protein (pseudogene)" FT /pseudo FT CDS 456418..456732 FT /class="II.C.2" FT /colour=3 FT /gene="ML0363" FT /note="Similar to M. tuberculosis Rv3444c, esat6-family FT protein, TR:O06261 (EMBL:Al123456) (100 aa); Fasta score FT E(): 3.3e-20, 71.2% identity in 73 aa overlap and others FT of the esat6 family e.g. esat6 itself, 6 kDa early FT secretory antigenic target, SW:ESA6_MYCTU (Q57165) (94 FT aa); Fasta score E(): 1.5, 28.4% identity in 67 aa FT overlap." FT /product="possible secreted protein" FT RBS 456971..456976 FT /note="possible RBS" FT CDS 456984..457427 FT /class="II.A.1" FT /colour=2 FT /gene="rplM" FT /gene="ML0364" FT /note="Similar to M. tuberculosis rplM, 50S ribosomal FT protein L13, SW:RL13_MYCTU (O06260) (147 aa); Fasta score FT E(): 0, 91.2% identity in 147 aa overlap. Previuosly FT sequenced as SW:RL13_MYCLE (P38014) (147 aa); Fasta score FT E(): 0, 99.3% identity in 147 aa overlap. Contains Pfam FT match to entry PF00572 Ribosomal_L13, Ribosomal protein FT L13. Contains PS00783 Ribosomal protein L13 signature." FT /product="50S ribosomal protein L13" FT /tb_orthologue="rplM" FT misc_feature 457026..457409 FT /colour=0 FT /note="Pfam match to entry PF00572 Ribosomal_L13, FT Ribosomal protein L13, score 289.30, E-value 4.7e-83" FT misc_feature 457296..457364 FT /colour=8 FT /note="PS00783 Ribosomal protein L13 signature" FT CDS 457424..457885 FT /class="II.A.1" FT /colour=2 FT /gene="rpsI" FT /gene="ML0365" FT /note="Similar to M. tuberculosis rpsI, 30S ribosomal FT protein S9, SW:RS9_MYCTU (O06259) (151 aa); Fasta score FT E(): 0, 83.2% identity in 155 aa overlap. Previously FT sequenced as SW:RS9_MYCLE (P40828) (153 aa); Fasta score FT E(): 0, 99.3% identity in 153 aa overlap. Contains Pfam FT match to entry PF00380 Ribosomal_S9, Ribosomal protein FT S9/S16. Contains PS00360 Ribosomal protein S9 signature." FT /product="30S ribosomal protein S9" FT /tb_orthologue="rpsI" FT misc_feature 457520..457882 FT /colour=0 FT /note="Pfam match to entry PF00380 Ribosomal_S9, Ribosomal FT protein S9/S16, score 250.20, E-value 3.2e-77" FT misc_feature 457697..457753 FT /colour=8 FT /note="PS00360 Ribosomal protein S9 signature" FT RBS 457979..457983 FT /note="possible RBS" FT misc_feature 457992..459284 FT /colour=0 FT /note="Pfam match to entry PF00408 PGM_PMM, FT Phosphoglucomutase/phosphomannomutase, score 339.10, FT E-value 4.8e-98" FT CDS 457992..459383 FT /class="I.A.1" FT /colour=7 FT /gene="mrsA" FT /gene="ML0366" FT /note="Similar to M. tuberculosis mrsA, Rv3441c, FT phosphoglucomutase or phosphomannomutase, TR:O06258 FT (EMBL:AL123456) (448 aa); Fasta score E(): 0, 87.6% FT identity in 445 aa overlap. Similar to many e.g. FT Salmonella typhimurium manB, phosphomannomutase, FT SW:MANB_SALTY (P26341) (264 aa); BlastP Expect 9.5. FT Previously sequenced as TR:Q49869 (EMBL:U00020) (463 aa); FT Fasta score E(): 0, 100.0% identity in 463 aa overlap. FT Contains Pfam match to entry PF00408 PGM_PMM, FT Phosphoglucomutase/phosphomannomutase. Contains PS00710 FT Phosphoglucomutase and phosphomannomutase phosphoserine FT signature." FT /note="Similar to ML0706 and ML0763" FT /product="putative phosphoglucomutase/phosphomannomutase" FT /tb_orthologue="mrsA" FT misc_feature 458277..458321 FT /colour=8 FT /note="PS00710 Phosphoglucomutase and phosphomannomutase FT phosphoserine signature" FT CDS 459447..459648 FT /class="V" FT /colour=0 FT /gene="ML0367" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3440c (Best blastx score 104)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS 459675..460544 FT /class="V" FT /colour=0 FT /gene="ML0368" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3439c (Best blastx score 221)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(461185..461541) FT /class="VI" FT /colour=8 FT /gene="ML0369" FT /ml_unique FT /note="Unknown function. Previously sequenced as TR:Q49877 FT (EMBL:U00020) (118 aa); Fasta score E(): 0, 100.0% FT identity in 118 aa overlap." FT /product="hypothetical protein" FT CDS complement(461945..462814) FT /class="V" FT /colour=10 FT /gene="ML0370" FT /note="Similar to M. tuberculosis Rv3438, conserved FT hypothetical protein, TR:O06255 (EMBL:AL123456) (280 aa); FT Fasta score E(): 0, 80.6% identity in 279 aa overlap. FT Previously sequenced as TR:Q49872 (EMBL:U00020) (324 aa); FT Fasta score E(): 0, 100.0% identity in 289 aa overlap. FT Contains PS00107 Protein kinases ATP-binding region FT signature." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv3438" FT misc_feature complement(462365..462442) FT /colour=8 FT /note="PS00107 Protein kinases ATP-binding region FT signature" FT CDS 462979..464856 FT /EC_number="2.6.1.16" FT /class="I.C.4" FT /colour=7 FT /gene="glmS" FT /gene="ML0371" FT /note="Similar to M. tuberculosis glmS, Rv3436c, putative FT glucosamine-fructose-6-phosphate aminotransferase, FT SW:GLMS_MYCTU (O06253) (623 aa); Fasta score E(): 0, 89.3% FT identity in 627 aa overlap. Similar to many e.g. Rhizobium FT leguminosarum nodM, glucosamine--fructose-6-phosphate FT aminotransferase, SW:NODM_RHILV (P08633) (607 aa); Fasta FT score E(): 0, 44.0% identity in 629 aa overlap. Previously FT sequenced as SW:GLMS_MYCLE (P40831) (624 aa); Fasta score FT E(): 0, 99.7% identity in 624 aa overlap. Contains Pfam FT match to entry PF00310 GATase_2, Glutamine FT amidotransferases class-II. Contains 2 Pfam matches to FT entry PF01380 SIS, SIS domain." FT /product="putative glucosamine-fructose-6-phosphate FT aminotransferase" FT /tb_orthologue="glmS" FT misc_feature 462982..463557 FT /colour=0 FT /note="Pfam match to entry PF00310 GATase_2, Glutamine FT amidotransferases class-II, score 213.60, E-value 3e-72" FT misc_feature 463873..464277 FT /colour=0 FT /note="Pfam match to entry PF01380 SIS, SIS domain, score FT 183.00, E-value 4.8e-51" FT misc_feature 464389..464811 FT /colour=0 FT /note="Pfam match to entry PF01380 SIS, SIS domain, score FT 148.10, E-value 1.5e-40" FT CDS complement(464951..465073) FT /class="V" FT /colour=0 FT /gene="ML0372" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv0893c (Best blastx score 92)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS 465202..466623 FT /class="V" FT /colour=10 FT /gene="ML0373" FT /note="Similar to M. tuberculosis Rv3433c, conserved FT hypothetical protein, SW:YY33_MYCTU (O06250) (473 aa); FT Fasta score E(): 0, 84.6% identity in 473 aa overlap. FT Similar to many bacterial hypothetical proteins. FT Previuosly sequenced as SW:YY33_MYCLE (P37391) (392 aa); FT Fasta score E(): 0, 94.5% identity in 381 aa overlap. FT Contains Pfam match to entry PF01256 UPF0031, FT Uncharacterized protein family UPF0031. Contains PS01049 FT Uncharacterized protein family UPF0031 signature 1. FT Contains PS01050 Uncharacterized protein family UPF0031 FT signature 2." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv3433c" FT misc_feature 465898..466596 FT /colour=0 FT /note="Pfam match to entry PF01256 UPF0031, FT Uncharacterized protein family UPF0031, score 319.30, FT E-value 4.6e-92" FT misc_feature 466075..466107 FT /colour=8 FT /note="PS01049 Uncharacterized protein family UPF0031 FT signature 1" FT misc_feature 466423..466455 FT /colour=8 FT /note="PS01050 Uncharacterized protein family UPF0031 FT signature 2" FT CDS 466653..468022 FT /class="I.C.1" FT /colour=0 FT /gene="gadB" FT /gene="ML0374" FT /note="Possible pseudogene of M. tuberculosis orthologue FT gadB (Best blastx score 527)" FT /product="glutamate decarboxylase (pseudogene)" FT /pseudo FT RBS 468117..468121 FT /note="possible RBS" FT CDS 468132..469298 FT /EC_number="5.1.1.1" FT /class="I.D.6" FT /colour=7 FT /gene="alr" FT /gene="ML0375" FT /note="Similar to M. tuberculosis alr, Rv3423c, putative FT alanine racemase, SW:ALR_MYCTU (Q50705) (384 aa); Fasta FT score E(): 0, 84.6% identity in 382 aa overlap. Similar to FT many e.g. Mycobacterium smegmatis alr, alanine racemase, FT SW:ALR_MYCSM (P94967) (389 aa); Fasta score E(): 0, 67.2% FT identity in 378 aa overlap. Previously sequenced as FT SW:ALR_MYCLE (P38056) (388 aa); Fasta score E(): 0, 99.7% FT identity in 388 aa overlap. Contains 2 Pfam matches to FT entry PF00842 Ala_racemase, Alanine racemase." FT /product="putative alanine racemase" FT /tb_orthologue="alr" FT misc_feature 468243..468395 FT /colour=0 FT /note="Pfam match to entry PF00842 Ala_racemase, Alanine FT racemase, score 72.80, E-value 5.6e-19" FT misc_feature 468534..469274 FT /colour=0 FT /note="Pfam match to entry PF00842 Ala_racemase, Alanine FT racemase, score 245.40, E-value 8.1e-70" FT CDS 469288..470289 FT /class="II.C.5" FT /colour=3 FT /gene="ML0376" FT /ml_unique FT /note="Unknown function. Contains hydrophobic, possible FT membrane-spanning region near the N-terminus." FT /product="putative membrane protein" FT RBS 470263..470267 FT /note="possible RBS" FT CDS 470282..470767 FT /class="V" FT /colour=10 FT /gene="ML0377" FT /note="Similar to M. tuberculosis Rv3422c, conserved FT hypothetical protein, SW:YY22_MYCTU (Q50706) (168 aa); FT Fasta score E(): 0, 77.4% identity in 146 aa overlap. FT Similar to many other bacterial hypothetical proteins. FT Previously sequenced as SW:YY22_MYCLE (Q49864) (161 aa); FT Fasta score E(): 0, 100.0% identity in 161 aa overlap. FT Contains PS00017 ATP/GTP-binding site motif A (P-loop)." FT misc_feature 470399..470422 FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT CDS 470764..471843 FT /class="IV.H" FT /colour=7 FT /gene="ML0378" FT /note="Similar to M. tuberculosis rimI, Rv3420c, putative FT ribosomal protein S18 acetyltransferase, TR:Q50708 FT (EMBL:Z77165) (158 aa); Fasta score E(): 0, 72.1% identity FT in 154 aa overlap in the C-terminus and to M. tuberculosis FT Rv3421c hypothetical protein, SW:YY21_MYCTU (Q50707) (211 FT aa); Fasta score E(): 0, 75.6% identity in 205 aa overlap FT in the N-terminus. Appears to be a fusion of two M. FT tuberculosis homologues. Similar to many hypothetical FT acetyltransferases. Previously sequenced as SW:YY21_MYCLE FT (Q49857) (359 aa); Fasta score E(): 0, 100.0% identity in FT 359 aa overlap. Contains Pfam match to entry PF00583 FT Acetyltransf, Acetyltransferase (GNAT) family." FT /product="putative acetyltransferase" FT /tb_orthologue="rimI" FT misc_feature 471403..471768 FT /colour=0 FT /note="Pfam match to entry PF00583 Acetyltransf, FT Acetyltransferase (GNAT) family, score 69.60, E-value FT 6.8e-17" FT RBS 471831..471835 FT /note="possible RBS" FT CDS 471840..472895 FT /EC_number="3.4.24.57" FT /class="II.B.3" FT /colour=7 FT /gene="gcp" FT /gene="ML0379" FT /note="Similar to M. tuberculosis gcp, putative FT O-sialoglycoprotein endopeptidase, SW:GCP_MYCTU (Q50709) FT (344 aa); Fasta score E(): 0, 86.1% identity in 345 aa FT overlap. Similar to many e.g. Pasteurella haemolytica gcp, FT O-sialoglycoprotein endopeptidase, SW:GCP_PASHA (P36175) FT (325 aa); Fasta score E(): 0, 45.8% identity in 319 aa FT overlap. previously sequenced as SW:GCP_MYCLE (P37969) FT (351 aa); Fasta score E(): 0, 100.0% identity in 351 aa FT overlap. Contains Pfam match to entry PF00814 FT Peptidase_M22, Glycoprotease family. Contains PS01016 FT Glycoprotease family signature." FT /product="putative O-sialoglycoprotein endopeptidase" FT /tb_orthologue="gcp" FT misc_feature 471864..472820 FT /colour=0 FT /note="Pfam match to entry PF00814 Peptidase_M22, FT Glycoprotease family, score 601.80, E-value 4e-177" FT misc_feature 472164..472226 FT /colour=8 FT /note="PS01016 Glycoprotease family signature" FT RBS 473163..473167 FT /note="possible RBS" FT CDS 473177..473479 FT /class="III.B" FT /colour=0 FT /gene="groES" FT /gene="ML0380" FT /note="Similar to M. tuberculosis groES, 10 kD chaperonin, FT SW:CH10_MYCTU (P09621) (99 aa); Fasta score E(): 3.6e-32, FT 89.9% identity in 99 aa overlap. Previously sequenced as FT SW:CH10_MYCLE (P24301) (99 aa); Fasta score E(): 0, 100.0% FT identity in 99 aa overlap. Contains Pfam match to entry FT PF00166 cpn10, Chaperonins 10 Kd subunit. Contains PS00681 FT Chaperonins cpn10 signature." FT /product="10 kD chaperonin" FT /tb_orthologue="groES" FT misc_feature 473186..473473 FT /colour=0 FT /note="Pfam match to entry PF00166 cpn10, Chaperonins 10 FT Kd subunit, score 180.70, E-value 2.4e-50" FT misc_feature 473192..473266 FT /colour=8 FT /note="PS00681 Chaperonins cpn10 signature" FT RBS 473547..473553 FT /note="possible RBS" FT CDS 473558..475171 FT /class="III.B" FT /colour=0 FT /gene="groEL1" FT /gene="ML0381" FT /note="Similar to M. tuberculosis groEL1, 60 kDa FT chaperonin 1, SW:CH61_MYCTU (Q59573) (539 aa); Fasta score FT E(): 0, 82.9% identity in 539 aa overlap. Previously FT sequenced as SW:CH61_MYCLE (P37578) (537 aa); Fasta score FT E(): 0, 100.0% identity in 537 aa overlap. Contains Pfam FT match to entry PF00118 cpn60_TCP1, TCP-1/cpn60 chaperonin FT family. Contains PS00296 Chaperonins cpn60 signature." FT /product="60 kDa chaperonin 1" FT /tb_orthologue="groEL1" FT misc_feature 473621..475129 FT /colour=0 FT /note="Pfam match to entry PF00118 cpn60_TCP1, TCP-1/cpn60 FT chaperonin family, score 825.30, E-value 2.2e-244" FT misc_feature 474764..474799 FT /colour=8 FT /note="PS00296 Chaperonins cpn60 signature" FT CDS complement(475771..476079) FT /class="I.J.1" FT /colour=9 FT /gene="whiB3" FT /gene="whmB" FT /gene="ML0382" FT /note="Similar to M. tuberculosis whiB3, Rv3416, putative FT regulatory protein, TR:Q50710 (EMBL:AL123456) (102 aa); FT Fasta score E(): 0, 86.3% identity in 102 aa overlap. FT Similar to many e.g. Streptomyces coelicolor whiD, FT developmental regulatory gene, TR:O88103 (EMBL:AJ010601) FT (112 aa); Fasta score E(): 1.1e-24, 61.8% identity in 102 FT aa overlap. Previously sequenced as TR:Q49871 FT (EMBL:U00015) (102 aa); Fasta score E(): 0, 100.0% FT identity in 102 aa overlap." FT /note="Similar to ML0760, ML0804 and ML2307" FT /product="putative transcriptional regulator" FT /tb_orthologue="whiB3" FT RBS complement(476089..476093) FT /note="possible RBS" FT CDS 476440..477285 FT /class="V" FT /colour=10 FT /gene="ML0383" FT /note="Similar to M. tuberculosis Rv3415c, conserved FT hypothetical protein, TR:Q50711 (EMBL:AL123456) (275 aa); FT Fasta score E(): 0, 73.6% identity in 277 aa overlap. FT Previously sequenced as TR:Q49858 (EMBL:U00020) (264 aa); FT Fasta score E(): 0, 99.6% identity in 263 aa overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv3415c" FT CDS 477371..477955 FT /class="II.A.7" FT /colour=0 FT /gene="sigD" FT /gene="ML0384" FT /note="Possible pseudogene of M. tuberculosis orthologue FT sigD (Best blastx score 312)" FT /product="ECF subfamily sigma subunit (pseudogene)" FT /pseudo FT CDS 477951..478698 FT /class="V" FT /colour=0 FT /gene="ML0385" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3413c (Best blastx score 318)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(480093..480506) FT /class="V" FT /colour=10 FT /gene="ML0386" FT /note="Similar to M. tuberculosis Rv3412, conserved FT hypothetical protein, SW:YY12_MYCTU (Q50714) (136 aa); FT Fasta score E(): 0, 93.4% identity in 136 aa overlap. FT Previously sequenced as SW:YY12_MYCLE (Q49742) (137 aa); FT Fasta score E(): 0, 99.3% identity in 137 aa overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv3412" FT RBS 480647..480651 FT /note="possible RBS" FT CDS 480661..482250 FT /EC_number="1.1.1.205" FT /class="I.F.1" FT /colour=7 FT /gene="guaB2" FT /gene="ML0387" FT /note="Similar to M. tuberculosis guaB2, Rv3411c, putative FT inosine-5'-monophosphate dehydrogenase, SW:IMDH_MYCTU FT (Q50715) (529 aa); Fasta score E(): 0, 92.4% identity in FT 529 aa overlap. Similar to many e.g. Escherichia coli FT guaB, inosine-5'-monophosphate dehydrogenase, FT SW:IMDH_ECOLI (P06981) (488 aa); Fasta score E(): 0, 54.3% FT identity in 490 aa overlap. Previously sequenced as FT SW:IMDH_MYCLE (Q49729) (529 aa); Fasta score E(): 0, FT 100.0% identity in 529 aa overlap. Contains 2 Pfam matches FT to entry PF00571 CBS, CBS domain. Contains Pfam match to FT entry PF00478 IMPDH_C, IMP dehydrogenase / GMP reductase C FT terminus. Contains Pfam match to entry PF01574 IMPDH_N, FT IMP dehydrogenase / GMP reductase N terminus." FT /note="Similar to ML2066 and shows weaker similarity to FT ML0388" FT /product="putative inosine-5'-monophosphate dehydrogenase" FT /tb_orthologue="guaB2" FT misc_feature 480772..481035 FT /colour=0 FT /note="Pfam match to entry PF01574 IMPDH_N, IMP FT dehydrogenase / GMP reductase N terminus, score 193.40, FT E-value 3.5e-54" FT misc_feature 481039..481200 FT /colour=0 FT /note="Pfam match to entry PF00571 CBS, CBS domain, score FT 43.40, E-value 5.1e-09" FT misc_feature 481222..481383 FT /colour=0 FT /note="Pfam match to entry PF00571 CBS, CBS domain, score FT 40.90, E-value 2.9e-08" FT misc_feature 481471..482160 FT /colour=0 FT /note="Pfam match to entry PF00478 IMPDH_C, IMP FT dehydrogenase / GMP reductase C terminus, score 348.80, FT E-value 5.9e-101" FT CDS 482274..483386 FT /class="I.F.1" FT /colour=7 FT /gene="guaB3" FT /gene="ML0388" FT /note="Similar to M. tuberculosis guaB3, Rv3410c, putative FT inosine-5'-monophosphate dehydrogenase, SW:YY10_MYCTU FT (Q50716) (375 aa); Fasta score E(): 0, 90.5% identity in FT 368 aa overlap. This ORF is similar in part to ML0387 and FT ML2066, which more closely resemble the experimentally FT characterised IMP-DH's (e.g. from Escherichia coli). This FT shorter homologue is conserved in several bacterial FT genera. Previously sequenced as SW:YY10_MYCLE (U00015) FT (375 aa); Fasta score E(): 0, 100.0% identity in 370 aa FT overlap. Contains Pfam match to entry PF00478 IMPDH_C, IMP FT dehydrogenase / GMP reductase C terminus." FT /note="Shows weak similarity to ML0387 and ML2066" FT /product="putative inosine-5'-monophosphate dehydrogenase" FT /tb_orthologue="guaB3" FT misc_feature 482712..483368 FT /colour=0 FT /note="Pfam match to entry PF00478 IMPDH_C, IMP FT dehydrogenase / GMP reductase C terminus, score -16.40, FT E-value 3.7e-06" FT CDS 483451..485160 FT /class="I.A.3" FT /colour=1 FT /gene="choD" FT /gene="ML0389" FT /note="Similar to M. tuberculosis choD, Rv3409c, putative FT cholesterol oxidase, TR:Q57307 (EMBL:Al123456) (578 aa); FT Fasta score E(): 0, 88.6% identity in 569 aa overlap. FT Similar to Streptomyces sp. (strain SA-COO) choA, FT cholesterol oxidase precursor, SW:CHOD_STRSQ (P12676) (546 FT aa); Fasta score E(): 0.013, 26.6% identity in 561 aa FT overlap. Previously sequenced as TR:Q59530 (EMBL:U00015) FT (585 aa); Fasta score E(): 0, 100.0% identity in 569 aa FT overlap." FT /product="putative cholesterol oxidase" FT /tb_orthologue="choD" FT CDS complement(485208..485600) FT /class="I.B.7" FT /colour=0 FT /gene="ML0390" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3406 (Best blastx score 382)" FT /product="possibe dioxygenase (pseudogene)" FT /pseudo FT CDS 485789..486124 FT /class="I.J.1" FT /colour=0 FT /gene="ML0391" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3405c (Best blastx score 162)" FT /product="putative transcriptional regulator (pseudogene)" FT /pseudo FT CDS complement(486263..488641) FT /class="IV.H" FT /colour=7 FT /gene="ML0392" FT /note="Similar to M. tuberculosis Rv3401, conserved FT hypothetical protein, SW:YY01_MYCTU (Q50724) (786 aa); FT Fasta score E(): 0, 91.4% identity in 782 aa overlap. FT Shows weak similarity to Lactobacillus sanfranciscensis FT mapA, maltose phosphorylase, TR:O87772 (EMBL:AJ224340) FT (753 aa); Fasta score E(): 1.4e-13, 29.0% identity in 769 FT aa overlap. Similar to many bacterial hypothetical FT proteins e.g. Streptomyces coelicolor SCF42.31C, putative FT glycosyl transferase, TR:CAB69693 (EMBL:AL137165) (792 FT aa); Fasta score E(): 0, 58.9% identity in 790 aa overlap. FT Previously sequenced as SW:YY01_MYCLE (Q49736) (792 aa); FT Fasta score E(): 0, 100.0% identity in 792 aa overlap" FT /product="putative sugar phosphorylase" FT /tb_orthologue="Rv3401" FT CDS complement(488655..489440) FT /class="I.C.3" FT /colour=7 FT /gene="ML0393" FT /note="Similar to M. tuberculosis Rv3400, hypothetical FT protein, SW:YY00_MYCTU (Q50725) (262 aa); Fasta score E(): FT 0, 74.4% identity in 262 aa overlap. Previously sequenced FT as SW:YY00_MYCLE (Q49741) (261 aa); Fasta score E(): 0, FT 100.0% identity in 261 aa overlap. Contains Pfam match to FT entry PF00702 Hydrolase, haloacid dehalogenase-like FT hydrolase." FT /product="putative hydrolase" FT /tb_orthologue="Rv3400" FT misc_feature complement(488733..489377) FT /colour=0 FT /note="Pfam match to entry PF00702 Hydrolase, haloacid FT dehalogenase-like hydrolase, score 145.80, E-value FT 7.9e-40" FT CDS complement(489915..490427) FT /class="VI" FT /colour=8 FT /gene="ML0394" FT /ml_unique FT /note="Unknown function. Possible CDS suggested by GC FT frameplot and codon usage plots." FT /product="hypothetical protein" FT RBS complement(490440..490445) FT /note="possible RBS" FT CDS 491043..492632 FT /EC_number="6.3.5.2" FT /class="I.F.1" FT /colour=7 FT /gene="guaA" FT /gene="ML0395" FT /note="Similar to M. tuberculosis guaA, Rv3396c, putative FT GMP synthase, SW:GUAA_MYCTU (Q50729) (525 aa); Fasta score FT E(): 0, 86.9% identity in 525 aa overlap. Similar to many FT e.g. Bacillus subtilis guaA, GMP synthase FT [glutamine-hydrolyzing], SW:GUAA_BACSU (P29727) (513 aa); FT Fasta score E(): 0, 50.7% identity in 515 aa overlap. FT Previously sequenced as SW:GUAA_MYCLE (P46810) (590 aa); FT Fasta score E(): 0, 100.0% identity in 529 aa overlap. FT Contains Pfam match to entry PF00958 GMP_synt_C, GMP FT synthase C terminal domain. Contains Pfam match to entry FT PF00117 GATase, Glutamine amidotransferase class-I. FT Contains PS00442 Glutamine amidotransferases class-I FT active site." FT /product="putative GMP synthase" FT /tb_orthologue="guaA" FT misc_feature 491094..491639 FT /colour=0 FT /note="Pfam match to entry PF00117 GATase, Glutamine FT amidotransferase class-I, score 195.90, E-value 6.5e-55" FT misc_feature 491304..491339 FT /colour=8 FT /note="PS00442 Glutamine amidotransferases class-I active FT site" FT misc_feature 492318..492629 FT /colour=0 FT /note="Pfam match to entry PF00958 GMP_synt_C, GMP FT synthase C terminal domain, score 209.80, E-value 4.1e-59" FT repeat_region complement(492656..493927) FT CDS complement(492714..493823) FT /class="V" FT /colour=10 FT /gene="ML0396" FT /note="Similar to M. tuberculosis Rv0046c, conserved FT hypothetical protein, TR:P71703 (EMBL:AL123456) (367 aa); FT Fasta score E(): 0, 91.8% identity in 366 aa overlap. FT Similar to other bacterial hypothetical proteins. FT C-terminal half show weak similarity to eukaryotic FT myo-inositol-1-phosphate synthases e.g. Candida albicans FT ino1, myo-inositol-1-phosphate synthase, SW:INO1_CANAL FT (P42800) (520 aa); Fasta score E(): 0.22, 24.4% identity FT in 242 aa overlap. Previously sequenced as TR:Q57240 FT (EMBL:U00015) (369 aa); Fasta score E(): 0, 100.0% FT identity in 369 aa overlap. Contains Pfam match to entry FT PF01658 Inos-1-P_synth, Myo-inositol-1-phosphate FT synthase." FT /note="Identical in sequence to ML2692" FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv0046c" FT misc_feature complement(492723..493781) FT /colour=0 FT /note="Pfam match to entry PF01658 Inos-1-P_synth, FT Myo-inositol-1-phosphate synthase, score 558.20, E-value FT 5.3e-164" FT CDS complement(493939..495747) FT /class="III.A" FT /colour=3 FT /gene="ML0397" FT /ml_unique FT /note="C-terminal half is similar to sugar permeases e.g. FT Escherichia coli AraH, L-arabinose transport system FT permease protein, SW:ARAH_ECOLI (P08532) (329 aa); Fasta FT score E(): 1.1e-26, 36.0% identity in 297 aa overlap. The FT N-terminal half shows weak similarity to other proteins FT containing PAS and DUF9 domains. There is a possiblity FT that this is a signal transduction protein. Previously FT sequenced as TR:Q49739 (EMBL:U00015) (577 aa); Fasta score FT E(): 0, 99.8% identity in 577 aa overlap. Contains FT hydrophobic, possible membrane-spanning regions in the FT C-terminal half. Contains Pfam match to entry PF00989 PAS, FT PAS domain. Contains Pfam match to entry PF00990 DUF9, FT Domain of unknown function. Contains PS01039 Bacterial FT extracellular solute-binding proteins, family 3 FT signature." FT /note="The C-terminal half is similar to ML1419. The FT N-terminal half shows some similarity to ML1750" FT /product="putative transporter protein" FT misc_feature complement(494128..494169) FT /colour=8 FT /note="PS01039 Bacterial extracellular solute-binding FT proteins, family 3 signature" FT misc_feature complement(494854..495348) FT /colour=0 FT /note="Pfam match to entry PF00990 DUF9, Domain of unknown FT function, score 238.20, E-value 1.1e-67" FT misc_feature complement(495592..495717) FT /colour=0 FT /note="Pfam match to entry PF00989 PAS, PAS domain, score FT 32.10, E-value 2.8e-07" FT CDS complement(496060..497097) FT /class="III.A" FT /colour=3 FT /gene="ML0398" FT /ml_unique FT /note="Similar to many D-ribose-binding proteins e.g. FT Bacillus subtilis rbsB, D-ribose-binding protein FT precursor, SW:RBSB_BACSU (P36949) (305 aa); Fasta score FT E(): 2e-16, 32.6% identity in 273 aa overlap. Previously FT sequenced as TR:Q49738 (EMBL:U00015) (345 aa); Fasta score FT E(): 0, 100.0% identity in 345 aa overlap. Contains FT probable N-terminal signal sequence. Contains Pfam match FT to entry PF00532 Peripla_BP_like, Periplasmic binding FT proteins and sugar binding domain of the LacI family.." FT /product="putative D-ribose-binding protein" FT misc_feature complement(496240..496995) FT /colour=0 FT /note="Pfam match to entry PF00532 Peripla_BP_like, FT Periplasmic binding proteins and sugar binding domain of FT the LacI family., score 23.60, E-value 3.4e-07" FT RBS complement(497104..497109) FT /note="possible RBS" FT CDS 497357..498001 FT /class="V" FT /colour=0 FT /gene="ML0399" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3395c (Best blastx score 208)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS 498082..499157 FT /class="V" FT /colour=0 FT /gene="ML0400" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3394c (Best blastx score 268)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(499303..499755) FT /class="III.A.4" FT /colour=0 FT /gene="ML0401" FT /note="Possible pseudogene similar to M. tuberculosis FT paralogue phoY2 (Best blastx score 152)" FT /product="phosphate transport system regulator FT (pseudogene)" FT /pseudo FT CDS complement(499941..500372) FT /class="II.C.2" FT /colour=0 FT /gene="ML0402" FT /note="Possible pseudogene similar to M. tuberculosis FT paralogue Rv1758 (Best blastx score 151)" FT /product="putative cutinase (pseudogene)" FT /pseudo FT CDS complement(500826..501747) FT /class="I.F.4" FT /colour=0 FT /gene="iunH" FT /gene="ML0403" FT /note="Possible pseudogene of M. tuberculosis orthologue FT iunH (Best blastx score 213)" FT /product="probable inosine-uridine preferring nucleoside FT (pseudogene)" FT /pseudo FT CDS 501850..502403 FT /class="I.H.2" FT /colour=0 FT /gene="cmaA1" FT /gene="ML0404" FT /note="Possible pseudogene of M. tuberculosis orthologue FT cmaA1 (Best blastx score 278)" FT /product="cyclopropanemycolic acid synthase (pseudogene)" FT /pseudo FT RBS 503199..503204 FT /note="possible RBS" FT CDS 503217..504401 FT /class="V" FT /colour=10 FT /gene="ML0405" FT /note="Similar to M. tuberculosis Rv3616c, conserved FT hypothetical protein, TR:O06267 (EMBL:AL123456) (392 aa); FT Fasta score E(): 0, 62.7% identity in 394 aa overlap and FT to Rv3864, hypothetical protein, TR:P96213 (EMBL:AL123456) FT (402 aa); Fasta score E(): 2.1e-15, 31.0% identity in 410 FT aa overlap. Previously sequenced as TR:Q49722 FT (EMBL:U00015) (394 aa); Fasta score E(): 0, 100.0% FT identity in 394 aa overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv3616c" FT CDS 504459..504779 FT /class="V" FT /colour=10 FT /gene="ML0406" FT /note="Similar to M. tuberculosis Rv3615c, conserved FT hypothetical protein, TR:O06268 (EMBL:AL123456) (103 aa); FT Fasta score E(): 4.1e-19, 60.9% identity in 92 aa overlap FT and to Rv3865, hypothetical protein, TR:P96212 FT (EMBL:AL123456) (103 aa); Fasta score E(): 0.00022, 31.1% FT identity in 103 aa overlap. Previously sequenced as FT TR:Q49723 (EMBL:U00015) (106 aa); Fasta score E(): 0, FT 100.0% identity in 106 aa overlap." FT /product="conserved hypothetical protein" FT CDS 504793..505443 FT /class="V" FT /colour=10 FT /gene="ML0407" FT /note="Similar to M. tuberculosis Rv3614c, conserved FT hypothetical protein, TR:O06269 (EMBL:AL123456) (184 aa); FT Fasta score E(): 0, 71.5% identity in 186 aa overlap and FT to Rv3867, hypothetical protein, TR:O69732 (EMBL:AL123456) FT (183 aa); Fasta score E(): 2.1e-28, 51.4% identity in 175 FT aa overlap. Previously sequenced as TR:Q49730 FT (EMBL:U00015) (216 aa); Fasta score E(): 0, 100.0% FT identity in 216 aa overlap." FT /note="Similar to ML0056" FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv3614c" FT CDS 505777..506544 FT /class="IV.B.1.b" FT /colour=0 FT /gene="ML0408" FT /note="Possible pseudogene similar to M. tuberculosis FT IS1081 transposase (Best blastx score 140)" FT /product="possible IS1081 transposase (pseudogene)" FT /pseudo FT CDS complement(506861..507532) FT /class="IV.B.1.a" FT /colour=0 FT /gene="ML0409" FT /note="Possible pseudogene similar to M. tuberculosis FT IS6110 transposase (Best blastx score 164)" FT /product="possible IS6110 transposase (pseudogene)" FT /pseudo FT RBS 508310..508315 FT /note="possible RBS" FT misc_feature 508327..508599 FT /colour=0 FT /note="Pfam match to entry PF00934 PE, PE family, score FT 37.90, E-value 4.3e-08" FT CDS 508327..508629 FT /class="IV.C.1.a" FT /colour=6 FT /gene="ML0410" FT /note="Similar to M. tuberculosis PE-family proteins e.g. FT Rv2107, PE-family protein, TR:O33243 (EMBL:AL123456) (98 FT aa); Fasta score E(): 4.7e-08, 44.7% identity in 85 aa FT overlap. Contains Pfam match to entry PF00934 PE, PE FT family." FT /note="Similar to ML0538, ML1183 and ML1414" FT /product="putative PE-family protein" FT RBS 508740..508745 FT /note="possible RBS" FT CDS 508755..509981 FT /class="IV.C.2" FT /colour=6 FT /gene="ML0411" FT /note="Similar to M. tuberculosis PPE-family proteins FT e.g. Rv2108, PPE-family protein, TR:P95315 (EMBL:AL123456) FT (243 aa); Fasta score E(): 5.8e-17, 34.4% identity in 212 FT aa overlap. M. leprae serine-rich antigen (25L) (45 kDa FT protein). Previously sequenced as SW:SRA_MYCLE (Q07297) FT (408 aa); Fasta score E(): 0, 100.0% identity in 408 aa FT overlap. Contains Pfam match to entry PF00823 PPE, PPE FT family." FT /note="Similar to ML0539, ML1182 and ML1991" FT /product="serine-rich antigen" FT misc_feature 508770..509246 FT /colour=0 FT /note="Pfam match to entry PF00823 PPE, PPE family, score FT 281.00, E-value 1.5e-80" FT CDS complement(510369..510981) FT /class="II.C.1" FT /colour=0 FT /gene="lpqD" FT /gene="ML0412" FT /note="Possible pseudogene of M. tuberculosis orthologue FT lpqD (Best blastx score 254)" FT /product="putative lipoprotein (pseudogene)" FT /pseudo FT CDS 511130..511983 FT /class="I.B.7" FT /colour=0 FT /gene="ML0413" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3389c (Best blastx score 301)" FT /product="putative dehydrogenase (pseudogene)" FT /pseudo FT CDS complement(511984..513273) FT /class="III.E" FT /colour=0 FT /gene="otsB2" FT /gene="ML0414" FT /note="Similar to M. tuberculosis otsB2, FT trehalose-6-phosphate phosphatase, TR:O50401 FT (EMBL:AL123456) (391 aa); Fasta score E(): 0, 67.1% FT identity in 425 aa overlap. Similar, except at N-terminus, FT to Arabidopsis thaliana tppB, trehalose-6-phosphate FT phosphatase, TR:O64897 (EMBL:AF007779) (374 aa); Fasta FT score E(): 2.2e-17, 31.1% identity in 315 aa overlap. FT Previously sequenced as TR:Q49734 (EMBL:U00015) (429 aa); FT Fasta score E(): 0, 100.0% identity in 429 aa overlap. FT Contains PS00402 Binding-protein-dependent transport FT systems inner membrane comp sign.." FT /product="putative trehalose-6-phosphate phosphatase" FT /tb_orthologue="otsB2" FT misc_feature complement(512485..512571) FT /colour=8 FT /note="PS00402 Binding-protein-dependent transport systems FT inner membrane comp sign." FT CDS complement(513499..514618) FT /class="V" FT /colour=0 FT /gene="ML0415" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3371c (Best blastx score 169)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS 514919..517123 FT /class="II.A.5" FT /colour=0 FT /gene="dnaE2" FT /gene="ML0416" FT /note="Possible pseudogene of M. tuberculosis orthologue FT dnaE2 (Best blastx score 538)" FT /product="DNA polymerase III [alpha] chain (pseudogene)" FT /pseudo FT CDS complement(517299..517583) FT /class="V" FT /colour=0 FT /gene="ML0417" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3369 (Best blastx score 151)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS 517644..518276 FT /class="I.B.7" FT /colour=7 FT /gene="ML0418" FT /note="Similar to M. tuberculosis Rv3368c, conserved FT hypothetical protein, TR:O50397 (EMBL:AL123456) (214 aa); FT Fasta score E(): 0, 81.9% identity in 210 aa overlap. FT Shows weak similarity Thermus aquaticus nox, NADH FT dehydrogenase, SW:NOX_THETH (X60110) (205 aa); Fasta score FT E(): 0.00023, 28.8% identity in 212 aa overlap. Previously FT sequenced as TR:O07697 (EMBL:Z97179) (210 aa); Fasta score FT E(): 0, 99.5% identity in 210 aa overlap. Contains Pfam FT match to entry PF00881 Nitroreductase, Nitroreductase FT family. Contains PS00402 Binding-protein-dependent FT transport systems inner membrane comp sign.." FT /product="putative oxidoreductase" FT /tb_orthologue="Rv3368c" FT misc_feature 517680..518150 FT /colour=0 FT /note="Pfam match to entry PF00881 Nitroreductase, FT Nitroreductase family, score 146.20, E-value 5.9e-40" FT misc_feature 518109..518195 FT /colour=8 FT /note="PS00402 Binding-protein-dependent transport systems FT inner membrane comp sign." FT CDS complement(518414..518890) FT /class="II.A.7" FT /colour=2 FT /gene="ML0419" FT /note="Similar to M. tuberculosis spoU, Rv3366, possible FT RNA methyltransferase, TR:O50394 (EMBL:AL123456) (154 aa); FT Fasta score E(): 0, 83.8% identity in 154 aa overlap. FT Shows weak similarity to Escherichia coli spoU, tRNA FT (guanosine-2'-o-)-methyltransferase, SW:TRMH_ECOLI FT (P19396) (229 aa); Fasta score E(): 4e-05, 30.4% identity FT in 148 aa overlap. Similar to many hypothetical RNA FT methyltranferases. Previously sequenced as TR:O07698 FT (EMBL:Z97179) (169 aa); Fasta score E(): 0, 100.0% FT identity in 158 aa overlap. Contains Pfam match to entry FT PF00588 SpoU_methylase, SpoU rRNA Methylase family." FT /product="putative tRNA/rRNA methyltransferase" FT /tb_orthologue="spoU" FT misc_feature complement(518453..518878) FT /colour=0 FT /note="Pfam match to entry PF00588 SpoU_methylase, SpoU FT rRNA Methylase family, score 120.50, E-value 3.1e-32" FT CDS 519275..521679 FT /class="V" FT /colour=0 FT /gene="ML0420" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3365c (Best blastx score 540)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS 521734..522126 FT /class="V" FT /colour=0 FT /gene="ML0421" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3364c (Best blastx score 181)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS 522247..522813 FT /class="V" FT /colour=0 FT /gene="ML0422" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3362c (Best blastx score 425)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS 522864..523111 FT /class="V" FT /colour=0 FT /gene="ML0423" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3361c (Best blastx score 162)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT repeat_region 523044..524077 FT CDS complement(523803..524288) FT /class="III.F" FT /colour=0 FT /gene="bcp" FT /gene="ML0424" FT /note="Similar to M. tuberculosis bcp, Rv2521, FT bacterioferritin comigratory protein, TR:O5322 FT (EMBL:AL123456) (157 aa); Fasta score E(): 0, 79.6% FT identity in 157 aa overlap. Similar to many members of the FT AhpC/TSA family, suggesting a protective, antioxidant FT role. e.g. shows similarity to Sedum lineare prxQ, FT peroxiredoxin Q, TR:BAA90524 (EMBL:AB037598) (186 aa); FT Fasta score E(): 3e-17, 40.0% identity in 155 aa overlap FT and weak similarity to Mus musculus tdpX1, thioredoxin FT peroxidase 1, SW:TDX1_MOUSE (Q61171) (343 aa); BlastP FT Expect 8.8. Previously sequenced as TR:O07705 FT (EMBL:Z97179) (161 aa); Fasta score E(): 0, 99.4% identity FT in 161 aa overlap. Contains Pfam match to entry PF00578 FT AhpC-TSA, AhpC/TSA family." FT /product="putative antioxidant protein" FT /tb_orthologue="bcp" FT misc_feature complement(523827..524264) FT /colour=0 FT /note="Pfam match to entry PF00578 AhpC-TSA, AhpC/TSA FT family, score 117.20, E-value 3.1e-31" FT RBS complement(524293..524297) FT /note="possible RBS" FT RBS 524403..524407 FT /note="possible RBS" FT CDS 524416..524643 FT /class="II.C.4" FT /colour=3 FT /gene="ML0425" FT /note="Similar to M. tuberculosis Rv2520c, hypothetical FT protein, TR:O53225 (EMBL:AL123456) (75 aa); Fasta score FT E(): 2.2e-15, 57.3% identity in 75 aa overlap. Previously FT sequenced as TR:O07706 (EMBL:Z97179) (91 aa); Fasta score FT E(): 7.9e-29, 100.0% identity in 75 aa overlap. Contains FT hydrophobic, possible membrane-spanning region." FT /product="putative membrane protein" FT tRNA complement(524675..524750) FT /colour=4 FT /note="tRNA Lys anticodon CTT, Cove score 84.12" FT CDS 524929..526143 FT /class="II.C.2" FT /colour=3 FT /gene="lppS" FT /gene="ML0426" FT /note="Similar to M. tuberculosis lppS, Rv2518c, putative FT lipoprotein, TR:O53223 (EMBL:AL021185) (408 aa); Fasta FT score E(): 0, 82.6% identity in 403 aa overlap. Does not FT contain a predicted membrane lipoprotein lipid attachment FT site (present in lppS), but does contain a possible FT N-terminal signal sequence. Similar to many predicted FT lipoproteins from Streptomyces coelicolor. Previously FT sequenced as TR:O07707 (EMBL:Z97179) (407 aa); Fasta score FT E(): 0, 100.0% identity in 404 aa overlap." FT /note="Similar to ML0569, ML2446 and ML2664" FT /product="putative secreted protein" FT /tb_orthologue="lppS" FT tRNA complement(526168..526243) FT /colour=4 FT /note="tRNA His anticodon GTG, Cove score 82.48" FT CDS complement(526294..526941) FT /EC_number="3.1.-.-" FT /class="II.B.1" FT /colour=7 FT /gene="ML0427" FT /note="Similar to M. tuberculosis orn, Rv2511, putative FT oligoribonuclease, SW:ORN_MYCTU (O06174) (215 aa); Fasta FT score E(): 0, 84.5% identity in 213 aa overlap. Similar to FT many e..g Escherichia coli orn, oligoribonuclease, FT SW:ORN_ECOLI (P39287) (180 aa); Fasta score E(): 8.2e-25, FT 45.3% identity in 172 aa overlap. Previously sequenced as FT SW:ORN_MYCLE (O07708) (215 aa); Fasta score E(): 0, 99.5% FT identity in 215 aa overlap." FT /product="putative oligoribonuclease" FT /tb_orthologue="Rv2511" FT CDS 527082..528557 FT /class="V" FT /colour=0 FT /gene="ML0428" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2510c (Best blastx score 399)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(528621..529427) FT /class="I.B.7" FT /colour=7 FT /gene="ML0429" FT /note="Similar to M. tuberculosis Rv2509, putative FT oxidoreductase, TR:O06172 (EMBL:AL123456) (268 aa); Fasta FT score E(): 0, 88.8% identity in 267 aa overlap. Similar to FT many putative dehydrogenases. Previously sequenced as FT TR:O07709 (EMBL:Z97179) (268 aa); Fasta score E(): 0, FT 100.0% identity in 268 aa overlap. Contains Pfam match to FT entry PF00106 adh_short, short chain dehydrogenase. FT Contains PS00061 Short-chain dehydrogenases/reductases FT family signature." FT /product="putative oxidoreductase" FT /tb_orthologue="Rv2509" FT misc_feature complement(528849..529400) FT /colour=0 FT /note="Pfam match to entry PF00106 adh_short, short chain FT dehydrogenase, score 103.40, E-value 4.3e-27" FT misc_feature complement(528912..528998) FT /colour=8 FT /note="PS00061 Short-chain dehydrogenases/reductases FT family signature" FT CDS 529520..530884 FT /class="II.C.4" FT /colour=3 FT /gene="ML0430" FT /note="Similar to M. tuberculosis Rv2508c, putative FT membrane protein, TR:O06171 (EMBL:AL123456) (445 aa); FT Fasta score E(): 0, 75.7% identity in 441 aa overlap. Also FT similar to Streptomyces coelicolor SC6D7.19C, putative FT integral membrane protein, TR:Q9RKX9 (EMBL:AL133213) (486 FT aa); Fasta score E(): 1.5e-13, 29.7% identity in 445 aa FT overlap. Previously sequenced as TR:O07710 (EMBL:Z97179) FT (464 aa); Fasta score E(): 0, 100.0% identity in 454 aa FT overlap. Contains hydrophobic, possible membrane-spanning FT regions." FT /product="putative membrane protein" FT /tb_orthologue="Rv2508c" FT CDS complement(530916..531695) FT /class="II.C.4" FT /colour=3 FT /gene="ML0431" FT /note="Similar to M. tuberculosis Rv2507, putative FT membrane protein, TR:O06170 (EMBL:AL123456) (273 aa); FT Fasta score E(): 0, 60.4% identity in 275 aa overlap. FT Previously sequenced as TR:O07711 (EMBL:Z97179) (261 aa); FT Fasta score E(): 0, 100.0% identity in 259 aa overlap. FT Contains hydrophobic, possible membrane-spanning regions." FT /product="putative membrane protein" FT /tb_orthologue="Rv2507" FT RBS complement(531699..531704) FT /note="possible RBS" FT CDS complement(531814..532464) FT /class="I.J.1" FT /colour=0 FT /gene="ML0432" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2506 (Best blastx score 173)" FT /product="transcriptional regulator (TetR/AcrR family) FT (pseudogene)" FT /pseudo FT CDS 532614..533912 FT /class="I.A.3" FT /colour=0 FT /gene="fadD35" FT /gene="ML0433" FT /note="Possible pseudogene of M. tuberculosis orthologue FT fadD35 (Best blastx score 403)" FT /product="acyl-CoA synthase (pseudogene)" FT /pseudo FT CDS 534033..534710 FT /class="I.A.3" FT /colour=0 FT /gene="scoA" FT /gene="ML0434" FT /note="Possible pseudogene of M. tuberculosis orthologue FT scoA (Best blastx score 439)" FT /product="3-oxo acid:CoA transferase, [alpha] subunit FT (pseudogene)" FT /pseudo FT CDS 534769..535414 FT /class="I.A.3" FT /colour=0 FT /gene="scoB" FT /gene="ML0435" FT /note="Possible pseudogene of M. tuberculosis orthologue FT scoB (Best blastx score 465)" FT /product="3-oxo acid:CoA transferase, [beta] subunit FT (pseudogene)" FT /pseudo FT CDS 535414..537000 FT /class="I.A.3" FT /colour=0 FT /gene="accD1" FT /gene="ML0436" FT /note="Possible pseudogene of M. tuberculosis orthologue FT accD1 (Best blastx score 396)" FT /product="acetyl/propionyl-CoA carboxylase, [beta] subunit FT (pseudogene)" FT /pseudo FT CDS 537009..538544 FT /class="I.A.3" FT /colour=0 FT /gene="accA1" FT /gene="ML0437" FT /note="Possible pseudogene of M. tuberculosis orthologue FT accA1 (Best blastx score 865)" FT /product="acetyl/propionyl-CoA carboxylase, [alpha] FT subunit (pseudogene)" FT /pseudo FT CDS 538598..539715 FT /class="I.A.3" FT /colour=0 FT /gene="fadE19" FT /gene="ML0438" FT /note="Possible pseudogene of M. tuberculosis orthologue FT fadE19 (Best blastx score 346)" FT /product="acyl-CoA dehydrogenase (aka mmgC) (pseudogene)" FT /pseudo FT CDS 539670..540204 FT /class="I.B.7" FT /colour=0 FT /gene="ML0439" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2499c (Best blastx score 164)" FT /product="putative aldehyde dehydrogenase (pseudogene)" FT /pseudo FT CDS 540204..540782 FT /class="I.B.3" FT /colour=0 FT /gene="citE" FT /gene="ML0440" FT /note="Possible pseudogene of M. tuberculosis orthologue FT citE (Best blastx score 389)" FT /product="citrate lyase [beta] chain (pseudogene)" FT /pseudo FT CDS 540802..541460 FT /class="I.B.2" FT /colour=0 FT /gene="pdhA" FT /gene="ML0441" FT /note="Possible pseudogene of M. tuberculosis orthologue FT pdhA (Best blastx score 325)" FT /product="pyruvate dehydrogenase E1 component [alpha] FT subunit (pseudogene)" FT /pseudo FT CDS 541506..542532 FT /class="I.B.2" FT /colour=0 FT /gene="pdhB" FT /gene="ML0442" FT /note="Possible pseudogene of M. tuberculosis orthologue FT pdhB (Best blastx score 387)" FT /product="pyruvate dehydrogenase E1 component [beta] FT subunit (pseudogene)" FT /pseudo FT CDS 542561..543749 FT /class="I.B.2" FT /colour=0 FT /gene="pdhC" FT /gene="ML0443" FT /note="Possible pseudogene of M. tuberculosis orthologue FT pdhC (Best blastx score 324)" FT /product="dihydrolipoamide acetyltransferase (pseudogene)" FT /pseudo FT CDS complement(544151..545199) FT /class="II.A.5" FT /colour=0 FT /gene="ML0444" FT /note="Possible pseudogene similar to M. tuberculosis FT paralogue Rv0071 (Best blastx score 239)" FT /product="group II intron maturase (pseudogene)" FT /pseudo FT repeat_unit complement(544404..545582) FT CDS complement(546027..546564) FT /class="IV.B.2" FT /colour=0 FT /gene="ML0445" FT /note="Possible pseudogene of M. tuberculosis REP-family FT protein (Best blastx score 218)" FT /product="REP-family protein (pseudogene)" FT /pseudo FT CDS complement(546688..547132) FT /class="I.A.3" FT /colour=0 FT /gene="ML0446" FT /note="Possible pseudogene similar to M. tuberculosis FT paralogue echA14 (Best blastx score 194)" FT /product="enoyl-CoA hydratase/isomerase superfamily FT (pseudogene)" FT /pseudo FT repeat_unit 547210..548062 FT CDS 547312..547788 FT /class="V" FT /colour=10 FT /gene="ML0447" FT /ml_unique FT /note="Similar to region of cytochrome P450s e.g. Bacillus FT megaterium CYP102A1, cytochrome P450(BM-3), SW:CPXB_BACME FT (P14779) (1048 aa); Fasta score E(): 0.00033, 31.8% FT identity in 132 aa overlap. previously sequenced as FT TR:O07142 (EMBL:Z96801) (126 aa); Fasta score E(): 0, FT 99.2% identity in 126 aa overlap." FT /product="hypothetical protein" FT CDS 547992..548264 FT /class="VI" FT /colour=8 FT /gene="ML0448" FT /ml_unique FT /note="Unknown function. Previously sequenced as TR:O07143 FT (EMBL:Z96801) (90 aa); Fasta score E(): 0, 100.0% identity FT in 90 aa overlap." FT /product="hypothetical protein" FT CDS complement(549366..550149) FT /class="I.H.1" FT /colour=0 FT /gene="tesB2" FT /gene="ML0449" FT /note="Possible pseudogene of M. tuberculosis orthologue FT tesB2 (Best blastx score 159)" FT /product="thioesterase II (pseudogene)" FT /pseudo FT CDS complement(550193..551116) FT /class="I.G.6" FT /colour=7 FT /gene="ML0450" FT /note="Similar to M. tuberculosis Rv2606c, hypothetical FT protein, SW:YQ06_MYCTU (O06208) (299 aa); Fasta score E(): FT 0, 89.7% identity in 291 aa overlap. Highly conserved in FT both bacteria and eukaryotes. Similar to Emericella FT nidulans pyroA, pyridoxine biosynthesis protein, TR:Q9UW83 FT (EMBL:AF133101) (304 aa); Fasta score E(): 0, 65.9% FT identity in 299 aa overlap. Previously sequenced as FT SW:YQ06_MYCLE (O07145) (333 aa); Fasta score E(): 0, FT 100.0% identity in 307 aa overlap. Contains Pfam match to FT entry PF01680 UPF0019, Uncharacterized protein family SNZ. FT Contains PS01235 Uncharacterized protein family UPF0019 FT signature." FT /product="putative pyridoxine biosynthesis protein" FT /tb_orthologue="Rv2606c" FT misc_feature complement(550409..550465) FT /colour=8 FT /note="PS01235 Uncharacterized protein family UPF0019 FT signature" FT misc_feature complement(550442..551062) FT /colour=0 FT /note="Pfam match to entry PF01680 UPF0019, FT Uncharacterized protein family SNZ, score 443.10, E-value FT 2.4e-129" FT CDS complement(551191..552240) FT /class="V" FT /colour=10 FT /gene="ML0451" FT /note="Similar to M. tuberculosis Rv2609c, conserved FT hypothetical protein, TR:O06205 (EMBL:AL123456) (351 aa); FT Fasta score E(): 0, 77.7% identity in 336 aa overlap. FT Similar, in part, to hypothetical proteins from FT Streptomyces coelicolor e.g. SC2E1.17, hypothetical FT protein, TR:O69888 (EMBL:AL023797) (172 aa); Fasta score FT E(): 2e-13, 43.3% identity in 150 aa overlap. Previously FT sequenced as TR:O07146 (EMBL:Z96801) (349 aa); Fasta score FT E(): 0, 100.0% identity in 349 aa overlap. Contains FT PS00893 mutT domain signature." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv2609c" FT misc_feature complement(551545..551604) FT /colour=8 FT /note="PS00893 mutT domain signature" FT CDS complement(552240..553364) FT /class="IV.H" FT /colour=7 FT /gene="ML0452" FT /note="Similar to M. tuberculosis Rv2610c putative FT glycosyltransferase, TR:O06204 (EMBL:AL123456) (378 aa); FT Fasta score E(): 0, 82.3% identity in 378 aa overlap. FT Similar to Synechococcus sp. sqdX, required for FT biosynthesis of the sulfolipid FT sulfoquinovosyldiacylglycerol, TR:Q9R6U1 (EMBL:U45308) FT (377 aa); Fasta score E(): 6.9e-12, 25.9% identity in 390 FT aa overlap and to CDS from the Bordetella parapertussis FT ipopolysaccharide biosynthesis locus e.g. wlbH, putative FT glcNac transferase, TR:O52848 (EMBL:AJ224768) (390 aa); FT Fasta score E(): 6.1e-12, 27.3% identity in 392 aa FT overlap. Previously sequenced as TR:O07147 (EMBL:Z96801) FT (374 aa); Fasta score E(): 0, 100.0% identity in 374 aa FT overlap. Contains Pfam match to entry PF00534 FT Glycos_transf_1, Glycosyl transferases group 1. Contains FT PS00017 ATP/GTP-binding site motif A (P-loop)." FT /note="Show weak similarity to ML0886, ML1715 and ML2443" FT /product="putative glycosyltransferase" FT /tb_orthologue="Rv2610c" FT misc_feature complement(552336..552800) FT /colour=0 FT /note="Pfam match to entry PF00534 Glycos_transf_1, FT Glycosyl transferases group 1, score 113.20, E-value FT 2.7e-30" FT misc_feature complement(552795..552818) FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT CDS complement(553374..554336) FT /class="V" FT /colour=10 FT /gene="ML0453" FT /note="Similar to M. tuberculosis Rv2611c, conserved FT hypothetical protein, TR:O06203 (EMBL:AL123456) (316 aa); FT Fasta score E(): 0, 71.5% identity in 312 aa overlap. FT Shows weak similarity to putative acyltransferases e.g. FT Campylobacter jejuni lipid A biosynthesis acyltransferase, FT TR:AAF31766 (EMBL:AF130984) (295 aa); Fasta score E(): FT 0.00017, 20.0% identity in 180 aa overlap. previously FT sequenced as TR:O07148 (EMBL:Z96801) (320 aa); Fasta score FT E(): 0, 99.7% identity in 320 aa overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv2611c" FT CDS complement(554333..555052) FT /class="I.H.3" FT /colour=1 FT /gene="pgsA" FT /gene="ML0454" FT /note="Similar to M. tuberculosis pgsA, Rv2612c, putative FT CDP-alcohol phosphatidyltransferases, TR:O06202 FT (EMBL:AL123456) (217 aa); Fasta score E(): 0, 79.3% FT identity in 213 aa overlap. Shows weak similarity to FT Escherichia coli pgsA, FT CDP-diacylglycerol--glycerol-3-phosphate FT 3-phosphatidyltransferase, SW:PGSA_ECOLI (P06978) (738 FT aa); BlastP Expect 8.6. Previously sequenced as TR:O07149 FT (EMBL:Z96801) (239 aa); Fasta score E(): 0, 100.0% FT identity in 239 aa overlap. Contains Pfam match to entry FT PF01066 CDP-OH_P_transf, CDP-alcohol FT phosphatidyltransferase. Contains PS00379 CDP-alcohol FT phosphatidyltransferases signature." FT /product="putative phosphatidyltransferase" FT /tb_orthologue="pgsA" FT misc_feature complement(554447..554881) FT /colour=0 FT /note="Pfam match to entry PF01066 CDP-OH_P_transf, FT CDP-alcohol phosphatidyltransferase, score 103.60, E-value FT 3.9e-27" FT misc_feature complement(554774..554842) FT /colour=8 FT /note="PS00379 CDP-alcohol phosphatidyltransferases FT signature" FT CDS complement(555049..555669) FT /class="V" FT /colour=10 FT /gene="ML0455" FT /note="Similar to M. tuberculosis Rv2613c, conserved FT hypothetical protein, TR:O06201 (EMBL:AL123456) (195 aa); FT Fasta score E(): 0, 84.7% identity in 196 aa overlap. FT Similar to many HIT-family hypothetical proteins. FT Previously sequenced as TR:O07150 (EMBL:Z96801) (186 aa); FT Fasta score E(): 0, 100.0% identity in 186 aa overlap. FT Contains Pfam match to entry PF01230 HIT, HIT family." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv2613c" FT misc_feature complement(555196..555507) FT /colour=0 FT /note="Pfam match to entry PF01230 HIT, HIT family, score FT 45.80, E-value 9.3e-10" FT CDS complement(555662..557770) FT /EC_number="6.1.1.3" FT /class="II.A.3" FT /colour=2 FT /gene="thrS" FT /gene="ML0456" FT /note="Similar to M. tuberculosis thrS, Rv2614c, FT threonyl-tRNA synthetase, SW:SYT_MYCTU (O06200) (692 aa); FT Fasta score E(): 0, 84.0% identity in 702 aa overlap. FT Similar to many e.g. Thermus aquaticus thrS, threonyl-tRNA FT synthetase, SW:SYT_THETH (P56881) (659 aa); Fasta score FT E(): 0, 45.0% identity in 645 aa overlap. Previously FT sequenced as SW:SYT_MYCLE (O07151) (702 aa); Fasta score FT E(): 0, 100.0% identity in 702 aa overlap. Contains Pfam FT match to entry PF00587 tRNA-synt_2b, tRNA synthetase class FT II (G, H, P, S and T). Contains PS00339 Aminoacyl-transfer FT RNA synthetases class-II signature 2. Contains PS00179 FT Aminoacyl-transfer RNA synthetases class-II signature 1." FT /product="threonyl-tRNA synthetase" FT /tb_orthologue="thrS" FT misc_feature complement(555767..556945) FT /colour=0 FT /note="Pfam match to entry PF00587 tRNA-synt_2b, tRNA FT synthetase class II (G, H, P, S and T), score 245.80, FT E-value 5.8e-70" FT misc_feature complement(556046..556075) FT /colour=8 FT /note="PS00339 Aminoacyl-transfer RNA synthetases class-II FT signature 2" FT misc_feature complement(556472..556537) FT /colour=8 FT /note="PS00179 Aminoacyl-transfer RNA synthetases class-II FT signature 1" FT CDS complement(557908..558313) FT /class="V" FT /colour=0 FT /gene="ML0457" FT /note="Possible pseudogene similar to M. tuberculosis FT Rv0356c (Best blastx score 97)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(558363..559268) FT /class="I.B.7" FT /colour=7 FT /gene="ML0458" FT /ml_unique FT /note="Similar to putative oxidoreductases from both FT bacteria and eukaryotes e.g. Schizosaccharomyces pombe FT SPBC215.11C, putative aldo-keto reductase, TR:O94315 FT (EMBL:AL033534) (306 aa); Fasta score E(): 0, 53.5% FT identity in 288 aa overlap. Previously sequenced as FT TR:O07152 (EMBL:Z96801) (306 aa); Fasta score E(): 0, FT 100.0% identity in 301 aa overlap. Contains 2 Pfam matches FT to entry PF00248 aldo_ket_red, Aldo/keto reductase FT family." FT /product="putative oxidoreductase" FT misc_feature complement(558378..558602) FT /colour=0 FT /note="Pfam match to entry PF00248 aldo_ket_red, Aldo/keto FT reductase family, score 14.70, E-value 0.0036" FT misc_feature complement(558741..558779) FT /colour=0 FT /note="Pfam match to entry PF00248 aldo_ket_red, Aldo/keto FT reductase family, score 1.00, E-value 28" FT CDS 559293..559666 FT /class="V" FT /colour=0 FT /gene="ML0459" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2616 (Best blastx score 154)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(559771..559989) FT /class="V" FT /colour=0 FT /gene="ML0460" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2617c (Best blastx score 163)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS 560205..560551 FT /class="V" FT /colour=0 FT /gene="ML0461" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2618 (Best blastx score 222)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(561078..561563) FT /class="V" FT /colour=0 FT /gene="ML0462" FT /note="Possible pseudogene similar to M. tuberculosis FT Rv0021c (Best blastx score 169)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(561579..561828) FT /class="V" FT /colour=0 FT /gene="ML0463" FT /note="Possible pseudogene similar to M. tuberculosis FT Rv2619c (Best blastx score 147)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS 561808..562095 FT /class="VI" FT /colour=8 FT /gene="ML0464" FT /ml_unique FT /note="Unknown function. Previously sequenced as TR:O07155 FT (EMBL:Z96801) (110 aa); Fasta score E(): 0, 100.0% FT identity in 95 aa overlap." FT /product="hypothetical protein" FT CDS 562650..563430 FT /class="IV.J" FT /colour=0 FT /gene="ML0465" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1320c (Best blastx score 85)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS 563632..564537 FT /class="II.C.2" FT /colour=3 FT /gene="ML0466" FT /ml_unique FT /note="Similar to Streptomyces coelicolor SC6A5.23, FT hypothetical protein, TR:Q9X7W9 (EMBL:AL049485) (261 aa); FT Fasta score E(): 9.5e-26, 36.4% identity in 253 aa FT overlap. Previously sequenced as TR:O07156 (EMBL:Z96801) FT (301 aa); Fasta score E(): 0, 99.7% identity in 301 aa FT overlap. Contains a possible N-terminal signal sequence." FT /product="possible secreted protein" FT CDS 564583..565227 FT /class="II.C.4" FT /colour=3 FT /gene="dedA" FT /gene="ML0467" FT /note="Similar to M. tuberculosis dedA, Rv2637, FT dedA-family protein, SW:YQ37_MYCTU (P71936) (218 aa); FT Fasta score E(): 0, 82.8% identity in 209 aa overlap. FT Similar to many dedA-family hypothetical proteins. FT Previously sequenced as SW:YQ37_MYCLE (Q49642) (214 aa); FT Fasta score E(): 0, 100.0% identity in 214 aa overlap. FT Contains hydrophobic, possible membrane-spanning regions. FT Contains Pfam match to entry PF00597 DedA, DedA family." FT /product="putative membrane protein" FT /tb_orthologue="dedA" FT misc_feature 564634..565119 FT /colour=0 FT /note="Pfam match to entry PF00597 DedA, DedA family, FT score 48.10, E-value 2e-10" FT CDS complement(566057..566839) FT /class="II.C.4" FT /colour=3 FT /gene="ML0468" FT /ml_unique FT /note="Similar to bacterial hypothetical proteins e.g. FT Aeropyrum pernix APE0183, hypothetical protein, TR:Q9YFR6 FT (EMBL:AP000058) (270 aa); Fasta score E(): 7.2e-07, 26.2% FT identity in 256 aa overlap. Contains hydrophobic, possible FT membrane-spanning regions." FT /product="putative membrane protein" FT CDS 567986..569438 FT /class="I.A.3" FT /colour=0 FT /gene="ML0469" FT /note="Possible pseudogene similar to M. tuberculosis FT fadD13 (Best blastx score 97)" FT /product="acyl-CoA synthase (pseudogene)" FT /pseudo FT CDS complement(571603..571893) FT /class="VI" FT /colour=8 FT /gene="ML0470" FT /ml_unique FT /note="Unknown function. Previously sequenced as TR:O07161 FT (EMBL:Z96801) (96 aa); Fasta score E(): 0, 100.0% identity FT in 96 aa overlap." FT /product="hypothetical protein" FT CDS 573279..573918 FT /class="III.A.2" FT /colour=0 FT /gene="ML0471" FT /note="Possible ferrous iron transport protein pseudogene" FT /product="ferrous iron transport protein (pseudogene)" FT /pseudo FT CDS 574032..574274 FT /class="VI" FT /colour=8 FT /gene="ML0472" FT /ml_unique FT /note="Improbable CDS suggested by GC frameplot and codon FT usage plots. Possibly the remains of a disrupted gene." FT /product="hypothetical protein" FT CDS 574538..574810 FT /class="VI" FT /colour=8 FT /gene="ML0473" FT /ml_unique FT /note="Improbable CDS suggested by GC frameplot and codon FT usage plots. Possibly the remains of a disrupted gene." FT /product="hypothetical protein" FT CDS 575469..576140 FT /class="V" FT /colour=10 FT /gene="ML0474" FT /note="Similar to M. tuberculosis Rv2604c, conserved FT hypothetical protein, TR:O06210 (EMBL:AL123456) (198 aa); FT Fasta score E(): 0, 82.8% identity in 198 aa overlap. FT Similar to many bacterial hypothetical proteins. Shows FT weak similarity to amidotransferases e.g. Streptomyces FT coelicolor hisH, amidotransferase from histidine FT biosynthetic pathway, SW:HIS5_STRCO (P16249) (213 aa); FT BlastP Expect 9.8. Previously sequenced as TR:Q49637 FT (EMBL:U00011) (219 aa); Fasta score E(): 0, 100.0% FT identity in 219 aa overlap. Contains Pfam match to entry FT PF01174 UPF0030, Uncharacterized protein family UPF0030." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv2604c" FT misc_feature 575565..576131 FT /colour=0 FT /note="Pfam match to entry PF01174 UPF0030, FT Uncharacterized protein family UPF0030, score 244.20, FT E-value 1.8e-69" FT CDS 576361..577116 FT /class="V" FT /colour=10 FT /gene="ML0475" FT /note="Similar to M. tuberculosis Rv2603c, conserved FT hypothetical protein, SW:YQ03_MYCTU (O33214) (251 aa); FT Fasta score E(): 0, 92.4% identity in 251 aa overlap . FT Similar to many bacterial hypothetical proteins. FT Previously sequenced as SW:YQ03_MYCLE (Q49645) (251 aa); FT Fasta score E(): 0, 100.0% identity in 251 aa overlap. FT Contains Pfam match to entry PF01709 DUF28, Domain of FT unknown function." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv2603c" FT misc_feature 576373..577077 FT /colour=0 FT /note="Pfam match to entry PF01709 DUF28, Domain of FT unknown function, score 446.90, E-value 1.7e-130" FT CDS complement(577202..579167) FT /class="I.E" FT /colour=0 FT /gene="speE" FT /gene="ML0476" FT /note="Possible pseudogene of M. tuberculosis orthologue FT speE, interrupted by insertion of an RLEP repeat (Best FT blastx score 229)" FT /product="spermidine synthase (pseudogene)" FT /pseudo FT repeat_region complement(577631..578776) FT CDS complement(579203..579570) FT /class="V" FT /colour=0 FT /gene="ML0477" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2600 (Best blastx score 202)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(579687..580079) FT /class="V" FT /colour=0 FT /gene="ML0478" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2599 (Best blastx score 115)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(580137..580325) FT /class="V" FT /colour=0 FT /gene="ML0479" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2598 (Best blastx score 204)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(580453..581050) FT /class="V" FT /colour=0 FT /gene="ML0480" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2597 (Best blastx score 175)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS 581224..581790 FT /EC_number="3.1.22.4" FT /class="II.A.5" FT /colour=2 FT /gene="ruvC" FT /gene="ML0481" FT /note="Similar to M. tuberculosis ruvC, Rv2594c, crossover FT junction endodeoxyribonuclease, SW:RUVC_MYCTU (Q50627) FT (188 aa); Fasta score E(): 0, 81.5% identity in 184 aa FT overlap. Similar to many e.g. Pseudomonas aeruginosa ruvC, FT crossover junction endodeoxyribonuclease, SW:RUVC_PSEAE FT (Q51424) (174 aa); Fasta score E(): 3.2e-15, 37.8% FT identity in 172 aa overlap. Previously sequenced as FT SW:RUVC_MYCLE (P40834) (188 aa); Fasta score E(): 0, FT 100.0% identity in 188 aa overlap. Contains Pfam match to FT entry PF02075 RuvC, Crossover junction FT endodeoxyribonuclease RuvC. Contains PS01321 Crossover FT junction endodeoxyribonuclease ruvC signature. Contains FT PS00572 Glycosyl hydrolases family 1 active site." FT /product="crossover junction endodeoxyribonuclease" FT /tb_orthologue="ruvC" FT misc_feature 581230..581679 FT /colour=0 FT /note="Pfam match to entry PF02075 RuvC, Crossover FT junction endodeoxyribonuclease RuvC, score 325.40, E-value FT 6.7e-94" FT misc_feature 581266..581292 FT /colour=8 FT /note="PS00572 Glycosyl hydrolases family 1 active site" FT misc_feature 581563..581670 FT /colour=8 FT /note="PS01321 Crossover junction endodeoxyribonuclease FT ruvC signature" FT CDS 581787..582398 FT /class="II.A.5" FT /colour=2 FT /gene="ruvA" FT /gene="ML0482" FT /note="Similar to M. tuberculosis ruvA, Rv2593c, Holliday FT junction DNA helicase, SW:RUVA_MYCTU (Q50628) (196 aa); FT Fasta score E(): 0, 76.4% identity in 203 aa overlap. FT Similar to many e.g. Escherichia coli ruvA, Holliday FT junction DNA helicase, SW:RUVA_ECOLI (P08576) (203 aa); FT Fasta score E(): 7e-14, 32.7% identity in 205 aa overlap. FT Previously sequenced as SW:RUVA_MYCLE (P40832) (203 aa); FT Fasta score E(): 0, 99.5% identity in 203 aa overlap. FT Contains Pfam match to entry PF01330 RuvA, Bacterial DNA FT recombination protein, RuvA." FT /product="Holliday junction DNA helicase component" FT /tb_orthologue="ruvA" FT misc_feature 581820..582386 FT /colour=0 FT /note="Pfam match to entry PF01330 RuvA, Bacterial DNA FT recombination protein, RuvA, score 319.80, E-value FT 3.2e-92" FT CDS 582395..583444 FT /class="II.A.5" FT /colour=2 FT /gene="ruvB" FT /gene="ML0483" FT /note="Similar to M. tuberculosis ruvB, Rv2592c, Holliday FT junction DNA helicase, SW:RUVB_MYCTU (Q50629) (344 aa); FT Fasta score E(): 0, 94.4% identity in 342 aa overlap. FT Similar to many e.g. Escherichia coli ruvB, Holliday FT junction DNA helicase, SW:RUVB_ECOLI (P08577) (336 aa); FT Fasta score E(): 0, 54.1% identity in 329 aa overlap. FT Previously sequenced as SW:RUVB_MYCLE (P40833) (369 aa); FT Fasta score E(): 0, 100.0% identity in 322 aa overlap. FT Contains Pfam match to entry PF00004 AAA, ATPases FT associated with various cellular activities (AAA). FT Contains PS00017 ATP/GTP-binding site motif A (P-loop)." FT /product="Holliday junction helicase component" FT /tb_orthologue="ruvB" FT misc_feature 582569..583156 FT /colour=0 FT /note="Pfam match to entry PF00004 AAA, ATPases associated FT with various cellular activities (AAA), score -14.30, FT E-value 0.00034" FT misc_feature 582584..582607 FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT CDS complement(584381..587947) FT /class="I.A.3" FT /colour=1 FT /gene="fadD9" FT /gene="ML0484" FT /note="Similar to M. tuberculosis fadD9, Rv2590, putative FT acyl-CoA synthetase, TR:Q5063 (EMBL:Al123456) (1168 aa); FT Fasta score E(): 0, 68.0% identity in 1178 aa overlap1. FT Similar to acyl-CoA ligases and to domains of FT polyketide/peptide synthetases e.g. Mycobacterium FT smegmatis mps, peptide synthetase, TR:Q9RLP6 FT (EMBL:AJ238027) (5990 aa); Fasta score E(): 0, 37.2% FT identity in 1168 aa overlap. C-terminal half is similar to FT eukaryotic aminoadipate-semialdehyde dehydrogenase e.g. FT Saccharomyces cerevisiae lys2, aminoadipate-semialdehyde FT dehydrogenase large subunit, SW:LYS2_YEAST (P07702) (1392 FT aa); Fasta score E(): 7.1e-18, 25.1% identity in 734 aa FT overlap. Previously sequenced as TR:O69484 (EMBL:AL023591) FT (1174 aa); Fasta score E(): 0, 100.0% identity in 1174 aa FT overlap. Contains Pfam match to entry PF00550 pp-binding, FT Phosphopantetheine attachment site. Contains Pfam match to FT entry PF00501 AMP-binding, AMP-binding enzyme. Contains FT PS00455 Putative AMP-binding domain signature. Contains FT PS00061 Short-chain dehydrogenases/reductases family FT signature." FT /product="putative acyl-CoA synthetase" FT /tb_orthologue="fadD9" FT misc_feature complement(585002..585088) FT /colour=8 FT /note="PS00061 Short-chain dehydrogenases/reductases FT family signature" FT misc_feature complement(585779..585940) FT /colour=0 FT /note="Pfam match to entry PF00550 pp-binding, FT Phosphopantetheine attachment site, score 33.60, E-value FT 1.2e-07" FT misc_feature complement(586286..587638) FT /colour=0 FT /note="Pfam match to entry PF00501 AMP-binding, FT AMP-binding enzyme, score -100.90, E-value 8.7e-10" FT misc_feature complement(587129..587164) FT /colour=8 FT /note="PS00455 Putative AMP-binding domain signature" FT CDS complement(588043..589383) FT /EC_number="2.6.1.19" FT /class="I.C.1" FT /colour=7 FT /gene="gabT" FT /gene="ML0485" FT /note="Similar to M. tuberculosis gabT, Rv2589, FT 4-aminobutyrate aminotransferase, SW:GABT_MYCTU (Q50632) FT (449 aa); Fasta score E(): 0, 83.7% identity in 449 aa FT overlap. Similar to many e.g. Escherichia coli gabT, FT 4-aminobutyrate aminotransferase, SW:GABT_ECOLI (P22256) FT (426 aa); Fasta score E(): 0, 43.1% identity in 422 aa FT overlap. Previously sequenced as SW:GABT_MYCLE (P40829) FT (446 aa); Fasta score E(): 0, 99.8% identity in 446 aa FT overlap. Contains Pfam match to entry PF00202 aminotran_3, FT Aminotransferases class-III pyridoxal-phosphate. Contains FT PS00600 Aminotransferases class-III pyridoxal-phosphate FT attachment site." FT /note="Similar to ML1216, ML1409 and ML2414" FT /product="putative 4-aminobutyrate aminotransferase" FT /tb_orthologue="gabT" FT misc_feature complement(588109..589245) FT /colour=0 FT /note="Pfam match to entry PF00202 aminotran_3, FT Aminotransferases class-III pyridoxal-phosphate, score FT 639.30, E-value 7.7e-222" FT misc_feature complement(588496..588609) FT /colour=8 FT /note="PS00600 Aminotransferases class-III FT pyridoxal-phosphate attachment site" FT RBS complement(589390..589395) FT /note="possible RBS" FT CDS 589652..589996 FT /class="II.C.2" FT /colour=3 FT /gene="ML0486" FT /note="Similar to M. tuberculosis Rv2588c, hypothetical FT protein, SW:YP88_MYCTU (Q50633) (115 aa); Fasta score E(): FT 7e-29, 77.0% identity in 100 aa overlap. Similar to FT bacterial hypothetical proteins e.g. Streptomyces FT coelicolor SCL2.07C, putative secreted protein, FT TR:CAB70919 (EMBL:AL137778) (169 aa); Fasta score E(): FT 7.3e-08, 35.8% identity in 120 aa overlap. Previously FT sequenced as SW:YP88_MYCLE (Q49647) (114 aa); Fasta score FT E(): 0, 100.0% identity in 114 aa overlap. Contains a FT possible N-terminal signal sequence." FT /product="putative secreted protein" FT CDS 590036..591829 FT /class="III.D" FT /colour=3 FT /gene="secD" FT /gene="ML0487" FT /note="Similar to M. tuberculosis secD, Rv2587c, putative FT protein-export membrane protein, SW:SECD_MYCTU (Q50634) FT (573 aa); Fasta score E(): 0, 80.6% identity in 583 aa FT overlap. C-terminal half is similar to the corresponding FT region of Escherichia coli secD, protein-export membrane FT protein, SW:SECD_ECOLI (P19673) (689 aa); BlastP Expect FT 0.42. Shows full length similarity to M. tuberculosis and FT Streptomyces coelicolor homolgues. Previously sequenced as FT SW:SECD_MYCLE (P38387) (571 aa); Fasta score E(): 0, 99.8% FT identity in 571 aa overlap. Contains hydrophobic, possible FT membrane-spanning regions." FT /product="putative protein-export membrane protein" FT /tb_orthologue="secD" FT RBS 591817..591822 FT /note="possible RBS" FT CDS 591834..593249 FT /class="III.D" FT /colour=3 FT /gene="secF" FT /gene="ML0488" FT /note="Similar to M. tuberculosis secF, Rv2586c, putative FT protein-export membrane protein, SW:SECF_MYCTU (Q50635) FT (442 aa); Fasta score E(): 0, 72.1% identity in 456 aa FT overlap. Similar to Escherichia coli secF, protein-export FT membrane protein, SW:SECF_ECOLI (P19674) (189 aa); BlastP FT Expect 8.5. Previously sequenced as SW:SECF_MYCLE (P38386) FT (394 aa); Fasta score E(): 0, 99.7% identity in 394 aa FT overlap. Contains hydrophobic, possible membrane-spanning FT regions." FT /product="putative protein-export membrane protein" FT /tb_orthologue="secF" FT CDS 593294..594961 FT /class="II.C.1" FT /colour=3 FT /gene="ML0489" FT /note="Similar to M. tuberculosis Rv2585c, hypothetical FT protein, SW:YP85_MYCTU (Q50636) (557 aa); Fasta score E(): FT 0, 78.9% identity in 546 aa overlap. Previously sequenced FT as SW:YP85_MYCLE (Q49646) (426 aa); Fasta score E(): 0, FT 98.8% identity in 332 aa overlap. Contains a probable FT N-terminal signal sequence. Contains PS00013 Prokaryotic FT membrane lipoprotein lipid attachment site." FT /product="putative lipoprotein" FT /tb_orthologue="Rv2585c" FT misc_feature 593348..593380 FT /colour=8 FT /note="PS00013 Prokaryotic membrane lipoprotein lipid FT attachment site" FT CDS 594985..595504 FT /class="I.F.4" FT /colour=0 FT /gene="apt" FT /gene="ML0490" FT /note="Possible pseudogene of M. tuberculosis orthologue FT apt (Best blastx score 222)" FT /product="adenine phosphoribosyltransferases (pseudogene)" FT /pseudo FT RBS 595594..595600 FT /note="possible RBS" FT CDS 595607..597970 FT /EC_number="2.7.6.5" FT /class="I.F.5" FT /colour=7 FT /gene="relA" FT /gene="ML0491" FT /note="Similar to M. tuberculosis relA, Rv2583c, putative FT GTP pyrophosphokinase, SW:RELA_MYCTU (Q50638) (790 aa); FT Fasta score E(): 0, 93.4% identity in 790 aa overlap. FT Similar to many e.g. Corynebacterium glutamicum relA, GTP FT pyrophosphokinase, SW:RELA_CORGL (O87331) (760 aa); Fasta FT score E(): 0, 66.0% identity in 756 aa overlap. Previously FT sequenced as SW:RELA_MYCLE (Q49640) (787 aa); Fasta score FT E(): 0, 99.9% identity in 787 aa overlap. Contains Pfam FT match to entry PF01842 ACT, ACT domain." FT /product="putative GTP pyrophosphokinase" FT /tb_orthologue="relA" FT misc_feature 597734..597952 FT /colour=0 FT /note="Pfam match to entry PF01842 ACT, ACT domain, score FT 49.70, E-value 6.3e-11" FT CDS complement(598029..598916) FT /EC_number="5.2.1.8" FT /class="II.A.6" FT /colour=2 FT /gene="ppiB" FT /gene="ML0492" FT /note="Similar to M. tuberculosis ppiB, Rv2582, putative FT peptidyl-prolyl cis-trans isomerase, SW:CYPB_MYCTU FT (Q50639) (308 aa); Fasta score E(): 0, 72.5% identity in FT 295 aa overlap. Shows weak similarity to part of FT Caenorhabditis elegans cvp-1, peptidyl-prolyl cis-trans FT isomerase 1, SW:CYP1_CAEEL (P52009) (192 aa); Fasta score FT E(): 0.00071, 36.4% identity in 165 aa overlap. Previously FT sequenced as SW:CYPB_MYCLE (P46697) (295 aa); Fasta score FT E(): 0, 99.7% identity in 295 aa overlap. Contains FT hydrophobic, possible membrane-spanning region. FT Alternatively, this may represent an N-terminal signal FT sequence, given teh potential start codon at codon 33. FT Contains Pfam match to entry PF00160 pro_isomerase, FT Cyclophilin type peptidyl-prolyl cis-trans isomerase." FT /product="putative peptidyl-prolyl cis-trans isomerase" FT /tb_orthologue="ppiB" FT misc_feature complement(598197..598538) FT /colour=0 FT /note="Pfam match to entry PF00160 pro_isomerase, FT Cyclophilin type peptidyl-prolyl cis-trans isomerase, FT score 42.80, E-value 2.4e-11" FT RBS complement(598927..598931) FT /note="possible RBS" FT CDS 599080..599736 FT /class="IV.I" FT /colour=7 FT /gene="ML0493" FT /note="Similar to M. tuberculosis Rv2581c, hypothetical FT protein, SW:YP81_MYCTU (Q50640) (224 aa); Fasta score E(): FT 0, 82.0% identity in 222 aa overlap. Similar to many FT bacterial hypothetical proteins. Previously sequenced as FT SW:YP81_MYCLE (Q49649) (218 aa); Fasta score E(): 0, 99.5% FT identity in 218 aa overlap. Contains Pfam match to entry FT PF00753 lactamase_B, Metallo-beta-lactamase superfamily." FT /note="Similar to ML1391 and ML1912" FT /product="putative hydrolase" FT /tb_orthologue="Rv2581c" FT misc_feature 599101..599676 FT /colour=0 FT /note="Pfam match to entry PF00753 lactamase_B, FT Metallo-beta-lactamase superfamily, score 169.30, E-value FT 6.3e-47" FT CDS 599740..601023 FT /EC_number="6.1.1.21" FT /class="II.A.3" FT /colour=2 FT /gene="hisS" FT /gene="ML0494" FT /note="Similar to M. tuberculosis hisS, Rv2580c, FT histidyl-tRNA synthase, SW:SYH_MYCTU (Q50641) (423 aa); FT Fasta score E(): 0, 85.9% identity in 417 aa overlap. FT Similar to many e.g. Escherichia coli hisS, histidyl-tRNA FT synthase, SW:SYH_ECOLI (P04804) (423 aa); Fasta score E(): FT 0, 42.1% identity in 413 aa overlap. Previously sequenced FT as SW:SYH_MYCLE (P46696) (427 aa); Fasta score E(): 0, FT 99.5% identity in 427 aa overlap. Contains Pfam match to FT entry PF00587 tRNA-synt_2b, tRNA synthetase class II (G, FT H, P, S and T). Contains PS00017 ATP/GTP-binding site FT motif A (P-loop). Contains PS00211 ABC transporters family FT signature." FT /product="histidyl-tRNA synthase" FT /tb_orthologue="hisS" FT misc_feature 599917..600966 FT /colour=0 FT /note="Pfam match to entry PF00587 tRNA-synt_2b, tRNA FT synthetase class II (G, H, P, S and T), score 292.60, FT E-value 5e-84" FT misc_feature 600691..600714 FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT misc_feature 600748..600792 FT /colour=8 FT /note="PS00211 ABC transporters family signature" FT CDS 601152..601597 FT /class="IV.C.1.b" FT /colour=0 FT /gene="PE_PGRS" FT /gene="ML0495" FT /note="Possible pseudogene similar to M. tuberculosis FT PE_PGRS genes (Best blastx score 176)" FT /product="PE_PGRS-family protein (pseudogene)" FT /pseudo FT CDS 601750..602325 FT /class="V" FT /colour=0 FT /gene="ML0496" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2578c (Best blastx score 339)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(602342..603849) FT /class="V" FT /colour=0 FT /gene="ML0497" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2577 (Best blastx score 414)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS 604110..604425 FT /class="V" FT /colour=0 FT /gene="ML0498" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2576c (Best blastx score 119)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(605196..606015) FT /class="V" FT /colour=0 FT /gene="ML0499" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2575 (Best blastx score 329)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(606108..606592) FT /class="V" FT /colour=0 FT /gene="ML0500" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2573 (Best blastx score 211)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT RBS 606830..606833 FT /note="possible RBS" FT CDS 606839..608608 FT /EC_number="6.1.1.12" FT /class="II.A.3" FT /colour=2 FT /gene="aspS" FT /gene="ML0501" FT /note="Similar to M. tuberculosis aspS, Rv2572c, FT aspartyl-tRNA synthetase, SW:SYD_MYCTU (Q50649) (596 aa); FT Fasta score E(): 0, 88.0% identity in 592 aa overlap. FT Similar to many e.g. Escherichia coli aspS, aspartyl-tRNA FT synthetase, SW:SYD_ECOLI (P21889) (590 aa); Fasta score FT E(): 0, 46.8% identity in 583 aa overlap. Previously FT sequenced as SW:SYD_MYCLE (P36429) (589 aa); Fasta score FT E(): 0, 99.8% identity in 589 aa overlap. Contains Pfam FT match to entry PF00152 tRNA-synt_2, tRNA synthetases class FT II (D, K and N). Contains PS00179 Aminoacyl-transfer RNA FT synthetases class-II signature 1." FT /product="aspartyl-tRNA synthetase" FT /tb_orthologue="aspS" FT misc_feature 607184..608515 FT /colour=0 FT /note="Pfam match to entry PF00152 tRNA-synt_2, tRNA FT synthetases class II (D, K and N), score -4.30, E-value FT 2.1e-11" FT misc_feature 607481..607534 FT /colour=8 FT /note="PS00179 Aminoacyl-transfer RNA synthetases class-II FT signature 1" FT CDS 608647..609029 FT /class="V" FT /colour=0 FT /gene="ML0502" FT /note="Possible pseudogene similar to M. tuberculosis FT Rv1558 (Best blastx score 72)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS 609984..610358 FT /class="IV.C.2" FT /colour=0 FT /gene="PPE" FT /gene="ML0503" FT /note="Possible pseudogene similar to M. tuberculosis PPE FT genes (Best blastx score 98)" FT /product="PPE-family protein (pseudogene)" FT /pseudo FT CDS complement(611087..612390) FT /class="IV.B.1.c" FT /colour=0 FT /gene="ML0504" FT /note="Possible pseudogene of transposase similar to FT Mycobacterium avium istA (Best blastx score 250)" FT /product="transposase (pseudogene)" FT /pseudo FT CDS complement(612560..613458) FT /class="IV.B.1.c" FT /colour=0 FT /gene="ML0505" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2424c a hypothetical protein found within an IS element FT (Best blastx score 206)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(614636..614999) FT /class="V" FT /colour=0 FT /gene="ML0506" FT /note="Possible pseudogene similar to M. tuberculosis FT Rv2570 (Best blastx score 169)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS 615180..617503 FT /class="V" FT /colour=0 FT /gene="ML0507" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2567 (Best blastx score 542)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS 617501..618442 FT /class="V" FT /colour=10 FT /gene="ML0508" FT /note="Similar to M. tuberculosis Rv2569c, conserved FT hypothetical protein, SW:YP69_MYCTU (Q50652) (314 aa); FT Fasta score E(): 0, 84.4% identity in 301 aa overlap and FT to other hypothetical proteins from M. tuberculosis. FT Contains Pfam match to entry PF01841 Transglut_core, FT Transglutaminase-like superfamily." FT /note="Similar to ML0607" FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv2569c" FT misc_feature 618068..618280 FT /colour=0 FT /note="Pfam match to entry PF01841 Transglut_core, FT Transglutaminase-like superfamily, score 80.40, E-value FT 3.6e-20" FT CDS 618435..619450 FT /class="V" FT /colour=0 FT /gene="ML0509" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2568c (Best blastx score 271)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS 619666..621087 FT /class="V" FT /colour=10 FT /gene="ML0510" FT /note="Similar to M. tuberculosis Rv2559c, conserved FT hypothetical protein, SW:YP59_MYCT (Q50739) (452 aa); FT Fasta score E(): 0, 83.4% identity in 452 aa overlapU. FT Similar to many hypothetical proteins in bacteria and FT eukaryotes e.g. Escherichia coli ycaJ, hypothetical FT protein, SW:YCAJ_ECOLI (P45526) (447 aa); Fasta score E(): FT 0, 42.7% identity in 417 aa overlap. Previously sequenced FT as TR:O69490 (EMBL:AL023591) (447 aa); Fasta score E(): 0, FT 99.8% identity in 447 aa overlap. Contains Pfam match to FT entry PF00004 AAA, ATPases associated with various FT cellular activities (AAA). Contains PS00017 FT ATP/GTP-binding site motif A (P-loop)." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv2559c" FT misc_feature 619930..620508 FT /colour=0 FT /note="Pfam match to entry PF00004 AAA, ATPases associated FT with various cellular activities (AAA), score -4.80, FT E-value 6.2e-05" FT misc_feature 619945..619968 FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT CDS complement(621128..621842) FT /class="V" FT /colour=0 FT /gene="ML0511" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2558 (Best blastx score 199)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS 622159..624885 FT /EC_number="6.1.1.7" FT /class="II.A.3" FT /colour=2 FT /gene="alaS" FT /gene="ML0512" FT /note="Similar to M. tuberculosis alaS, Rv2555c, FT alanyl-tRNA synthetase, SW:SYA_MYCTU (O07438) (904 aa); FT Fasta score E(): 0, 84.7% identity in 907 aa overlap. FT Similar to many e.g. Homo sapiens aarS, alanyl-tRNA FT synthetase, SW:SYA_HUMAN (P49588) (968 aa); Fasta score FT E(): 0, 35.7% identity in 971 aa overlap. Contains Pfam FT match to entry PF01411 tRNA-synt_2c, tRNA synthetases FT class II (A). Contains PS00339 Aminoacyl-transfer RNA FT synthetases class-II signature 2." FT /product="alanyl-tRNA synthetase" FT /tb_orthologue="alaS" FT misc_feature 622171..624849 FT /colour=0 FT /note="Pfam match to entry PF01411 tRNA-synt_2c, tRNA FT synthetases class II (A), score 993.10, E-value 6.5e-295" FT misc_feature 622849..622878 FT /colour=8 FT /note="PS00339 Aminoacyl-transfer RNA synthetases class-II FT signature 2" FT CDS 624886..625440 FT /class="V" FT /colour=10 FT /gene="ML0513" FT /note="Similar to M. tuberculosis Rv2554c, hypothetical FT protein, SW:YP54_MYCTU (P94999) (170 aa); Fasta score E(): FT 0, 72.0% identity in 161 aa overlap. Similar to many FT bacterial hypothetical proteins e.g. Streptomyces FT coelicolor SC9C5.24C, hypothetical protein, TR:CAB93380 FT (EMBL:AL357523) (167 aa); Fasta score E(): 8.9e-25, 54.8% FT identity in 155 aa overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv2554c" FT RBS 625420..625423 FT /note="possible RBS" FT CDS 625433..626698 FT /class="II.C.4" FT /colour=3 FT /gene="ML0514" FT /note="Similar to M. tuberculosis Rv2553c, hypothetical FT protein, TR:P95000 (EMBL:AL123456) (417 aa); Fasta score FT E(): 0, 72.7% identity in 414 aa overlap. C-terminal half FT is similar to e.g. Streptomyces sphaeroides novB, gene FT from novobiocin biosynthetic gene cluster, TR:AAF67495 FT (EMBL:AF170880) (284 aa); Fasta score E(): 2.5e-22, 40.0% FT identity in 210 aa overlap. Shows full-length similarity FT to other hypothetical proteins e.g. Bacillus subtilis FT yrrL, hypothetical protein, TR:O34758 (EMBL:Z99117) (360 FT aa); Fasta score E(): 2.8e-10, 26.3% identity in 358 aa FT overlap. Contains hydrophobic, possible membrane-spanning FT region near the N-terminus." FT /product="putative membrane protein" FT /tb_orthologue="Rv2553c" FT CDS 626676..627512 FT /class="I.D.4" FT /colour=7 FT /gene="aroE" FT /gene="ML0515" FT /note="Similar to M. tuberculosis aroE, Rv2552c, shikimate FT 5-dehydrogenase, TR:P95001 (EMBL:AL123456) (269 aa); Fasta FT score E(): 0, 81.5% identity in 270 aa overlap. Similar to FT many e.g. Neisseria meningitidis aroE, shikimate FT dehydrogenase, TR:P95388 (EMBL:U82841) (269 aa); Fasta FT score E(): 2.5e-12, 29.6% identity in 274 aa overlap. FT Contains Pfam match to entry PF01488 Shikimate_DH, FT Shikimate / quinate 5-dehydrogenase." FT /product="putative shikimate 5-dehydrogenase" FT /tb_orthologue="aroE" FT misc_feature 626760..627464 FT /colour=0 FT /note="Pfam match to entry PF01488 Shikimate_DH, Shikimate FT / quinate 5-dehydrogenase, score 229.00, E-value 6.9e-65" FT RBS 627730..627733 FT /note="possible RBS" FT CDS 627741..628964 FT /EC_number="4.6.1.4" FT /class="I.D.4" FT /colour=7 FT /gene="aroF" FT /gene="ML0516" FT /note="Similar to M. tuberculosis aroF, Rv2540c, FT chorismate synthase, SW:AROC_MYCTU (P95013) (401 aa); FT Fasta score E(): 0, 88.0% identity in 401 aa overlap. FT Similar to many e.g. Staphylococcus aureus aroC, FT chorismate synthase, SW:AROC_STAAU (Q59803) (388 aa); FT Fasta score E(): 0, 43.1% identity in 383 aa overlap. FT Contains Pfam match to entry PF01264 Chorismate_synt, FT Chorismate synthase. Contains PS00788 Chorismate synthase FT signature 2. Contains PS00789 Chorismate synthase FT signature 3. Contains PS00787 Chorismate synthase FT signature 1." FT /product="putative chorismate synthase" FT /tb_orthologue="aroF" FT misc_feature 627744..628850 FT /colour=0 FT /note="Pfam match to entry PF01264 Chorismate_synt, FT Chorismate synthase, score 695.80, E-value 8.2e-212" FT misc_feature 627762..627809 FT /colour=8 FT /note="PS00787 Chorismate synthase signature 1" FT misc_feature 628140..628184 FT /colour=8 FT /note="PS00788 Chorismate synthase signature 2" FT misc_feature 628761..628811 FT /colour=8 FT /note="PS00789 Chorismate synthase signature 3" FT CDS 628972..629571 FT /EC_number="2.7.1.71" FT /class="I.D.4" FT /colour=7 FT /gene="aroK" FT /gene="ML0517" FT /note="Similar to M. tuberculosis aroK, Rv2539c, shikimate FT kinase, SW:AROK_MYCTU (P95014) (176 aa); Fasta score E(): FT 0, 79.6% identity in 167 aa overlap. Similar to many e.g. FT Escherichia coli aroK, shikimate kinase I, SW:AROK_ECOLI FT (P24167) (172 aa); Fasta score E(): 3.5e-13, 38.0% FT identity in 166 aa overlap. Contains Pfam match to entry FT PF01202 SKI, Shikimate kinase. Contains PS00017 FT ATP/GTP-binding site motif A (P-loop). Contains PS01128 FT Shikimate kinase signature." FT /product="putative shikimate kinase" FT /tb_orthologue="aroK" FT misc_feature 628978..629472 FT /colour=0 FT /note="Pfam match to entry PF01202 SKI, Shikimate kinase, FT score 226.20, E-value 4.9e-64" FT misc_feature 628996..629019 FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT misc_feature 629143..629220 FT /colour=8 FT /note="PS01128 Shikimate kinase signature" FT RBS 629557..629561 FT /note="possible RBS" FT CDS 629568..630653 FT /EC_number="4.6.1.3" FT /class="I.D.4" FT /colour=7 FT /gene="aroB" FT /gene="ML0518" FT /note="Similar to M. tuberculosis aroB, Rv2538c, FT 3-dehydroquinate synthase, SW:AROB_MYCTU (P36919) (362 FT aa); Fasta score E(): 0, 87.3% identity in 361 aa overlap. FT Contains Pfam match to entry PF01761 DHQ_synthase, FT 3-dehydroquinate synthase." FT /product="putative 3-dehydroquinate synthase" FT /tb_orthologue="aroB" FT misc_feature 629628..630638 FT /colour=0 FT /note="Pfam match to entry PF01761 DHQ_synthase, FT 3-dehydroquinate synthase, score 601.80, E-value 4.1e-177" FT CDS 630653..631090 FT /EC_number="4.2.1.10" FT /class="I.D.4" FT /colour=7 FT /gene="aroD" FT /gene="ML0519" FT /note="Similar to M. tuberculosis aroD, 3-dehydroquinate FT dehydratase, SW:AROQ_MYCTU (P36918) (146 aa); Fasta score FT E(): 0, 85.9% identity in 142 aa overlap. Similar to many FT e.g. Corynebacterium pseudotuberculosis aroQ, FT 3-dehydroquinate dehydratase, SW:AROQ_CORPS (P96750) (146 FT aa); Fasta score E(): 6.7e-33, 61.0% identity in 136 aa FT overlap. Contains Pfam match to entry PF01220 FT DHquinase_II, Dehydroquinase class II. Contains PS01029 FT Dehydroquinase class II signature." FT /product="3-dehydroquinate dehydratase" FT /tb_orthologue="aroD" FT misc_feature 630656..631087 FT /colour=0 FT /note="Pfam match to entry PF01220 DHquinase_II, FT Dehydroquinase class II, score 300.70, E-value 1.8e-86" FT misc_feature 630671..630724 FT /colour=8 FT /note="PS01029 Dehydroquinase class II signature" FT CDS complement(631179..631787) FT /class="II.C.4" FT /colour=3 FT /gene="ML0520" FT /note="Similar to M. tuberculosis Rv2536, possible FT membrane protein, TR:P95017 (EMBL:AL123456) (230 aa); FT Fasta score E(): 0, 63.2% identity in 201 aa overlap. FT Contains hydrophobic, possible membrane-spanning regions. FT Contains PS00013 Prokaryotic membrane lipoprotein lipid FT attachment site8." FT /product="putative membrane protein" FT misc_feature complement(631395..631427) FT /colour=8 FT /note="PS00013 Prokaryotic membrane lipoprotein lipid FT attachment site8" FT RBS complement(631792..631795) FT /note="possible RBS" FT CDS 631821..632951 FT /class="II.B.3" FT /colour=7 FT /gene="pepQ" FT /gene="ML0521" FT /note="Similar to M. tuberculosis pepQ, Rv2535c, putative FT cytoplasmic peptidase, TR:P95018 (EMBL:AL123456) (372 aa); FT Fasta score E(): 0, 83.0% identity in 376 aa overlap. FT Similar to many e.g. Pyrococcus furiosus cobalt-dependent FT proline dipeptidase, TR:P81535 (EMBL:AF060010) (348 aa); FT Fasta score E(): 1.6e-29, 33.7% identity in 359 aa FT overlap. Contains Pfam match to entry PF00557 FT Peptidase_M24, metallopeptidase family M24. Contains FT PS00491 Aminopeptidase P and proline dipeptidase FT signature. Contains PS00211 ABC transporters family FT signature." FT /product="putative cytoplasmic peptidase" FT /tb_orthologue="pepQ" FT misc_feature 632217..632906 FT /colour=0 FT /note="Pfam match to entry PF00557 Peptidase_M24, FT metallopeptidase family M24, score 200.70, E-value FT 2.3e-56" FT misc_feature 632454..632498 FT /colour=8 FT /note="PS00211 ABC transporters family signature" FT misc_feature 632694..632732 FT /colour=8 FT /note="PS00491 Aminopeptidase P and proline dipeptidase FT signature" FT RBS 632950..632954 FT /note="possible RBS" FT misc_feature 632967..633521 FT /colour=0 FT /note="Pfam match to entry PF01132 EFP, Elongation factor FT P (EF-P), score 438.40, E-value 6.2e-128" FT CDS 632967..633530 FT /class="II.A.6" FT /colour=2 FT /gene="efp" FT /gene="ML0522" FT /note="Similar to M. tuberculosis efp, Rv2534c, elongation FT factor P, SW:EFP_MYCTU (P95019) (187 aa); Fasta score E(): FT 0, 94.1% identity in 186 aa overlap. Similar to many e.g. FT Brevibacterium lactofermentum efp, elongation factor P, FT SW:EFP_BRELA (X99289) (187 aa); Fasta score E(): 0, 68.8% FT identity in 186 aa overlap. Contains Pfam match to entry FT PF01132 EFP, Elongation factor P (EF-P). Contains PS01275 FT Elongation factor P signature." FT /product="elongation factor P" FT /tb_orthologue="efp" FT misc_feature 633414..633473 FT /colour=8 FT /note="PS01275 Elongation factor P signature" FT CDS 633533..634105 FT /class="II.A.7" FT /colour=2 FT /gene="nusB" FT /gene="ML0523" FT /note="Similar to M. tuberculosis nusB, putative FT transcription termination protein, SW:NUSB_MYCTU (P95020) FT (156 aa); Fasta score E(): 0, 75.7% identity in 148 aa FT overlap. Similar to many e.g. Escherichia coli nusB, N FT utilization substance protein B, protein involved in FT regulation of transcription termination, SW:NUSB_ECOLI FT (P04381) (139 aa); Fasta score E(): 7.4e-08, 38.1% FT identity in 139 aa overlap. Contains Pfam match to entry FT PF01029 NusB, NusB family." FT /product="putative transcription termination protein" FT /tb_orthologue="nusB" FT misc_feature 633551..633943 FT /colour=0 FT /note="Pfam match to entry PF01029 NusB, NusB family, FT score 172.50, E-value 7.2e-48" FT RBS 634630..634636 FT /note="possible RBS" FT CDS 634643..637495 FT /class="I.A.2" FT /colour=7 FT /gene="adi" FT /gene="ML0524" FT /note="Similar to M. tuberculosis adi, Rv2531c, putative FT ornithine/arginine decarboxylase, TR:P95022 FT (EMBL:AL123456) (947 aa); Fasta score E(): 0, 86.4% FT identity in 951 aa overlap. Similar to decarboxylases of FT ornithine and lysine e.g. Escherichia coli speC, ornithine FT decarboxylase, constitutive, SW:DCOR_ECOLI (P21169) (731 FT aa); Fasta score E(): 3e-16, 26.8% identity in 650 aa FT overlap. Contains Pfam match to entry PF01276 OKR_DC_1, FT Orn/Lys/Arg decarboxylase." FT /product="putative amino acid decarboxylase" FT /tb_orthologue="adi" FT misc_feature 635513..637351 FT /colour=0 FT /note="Pfam match to entry PF01276 OKR_DC_1, Orn/Lys/Arg FT decarboxylase, score 169.50, E-value 5.4e-47" FT CDS 637492..637668 FT /class="V" FT /colour=10 FT /gene="ML0525" FT /note="Unknown function. Similar to M. tuberculosis FT Rv2493, hypothetical protein, TR:O53218 (EMBL:AL123456) FT (73 aa); Fasta score E(): 0.059, 47.7% identity in 44 aa FT overlap." FT /product="conserved hypothetical protein" FT CDS complement(638094..638979) FT /class="I.B.7" FT /colour=0 FT /gene="ML0526" FT /note="Possible pseudogene similar to M. tuberculosis FT Rv0575c (Best blastx score 73)" FT /product="possible oxidoreductase (pseudogene)" FT /pseudo FT CDS complement(639678..639932) FT /class="VI" FT /colour=8 FT /gene="ML0527" FT /ml_unique FT /note="Unknown function." FT /product="hypothetical protein" FT CDS complement(641810..642795) FT /class="II.C.3" FT /colour=0 FT /gene="ML0528" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1367c (Best blastx score 289)" FT /product="probable penicillin binding protein FT (pseudogene)" FT /pseudo FT CDS complement(642925..643496) FT /class="IV.H" FT /colour=0 FT /gene="ML0529" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1377c (Best blastx score 217)" FT /product="some similarity to methyltransferases FT (pseudogene)" FT /pseudo FT CDS complement(643652..645020) FT /class="V" FT /colour=0 FT /gene="ML0530" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1378c (Best blastx score 627)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS 645041..645572 FT /class="I.J.1" FT /colour=0 FT /gene="pyrR" FT /gene="ML0531" FT /note="Possible pseudogene of M. tuberculosis orthologue FT pyrR (Best blastx score 355)" FT /product="regulatory protein pyrimidine biosynthesis FT (pseudogene)" FT /pseudo FT CDS 645581..646546 FT /EC_number="2.1.3.2" FT /class="I.F.2" FT /colour=7 FT /gene="pyrB" FT /gene="ML0532" FT /note="Similar to M. tuberculosis pyrB, Rv1380, aspartate FT carbamoyltransferase, SW:PYRB_MYCTU (P71808) (319 aa); FT Fasta score E(): 0, 89.9% identity in 318 aa overlap. FT Similar to many e.g. Pseudomonas putida, aspartate FT carbamoyltransferase, SW:PYRB_PSEPU (Q59711) (334 aa); FT Fasta score E(): 0, 47.3% identity in 317 aa overlap. FT Contains 2 Pfam matches to entry PF00185 OTCace, FT Aspartate/ornithine carbamoyltransferase. Contains PS00097 FT Aspartate and ornithine carbamoyltransferases signature." FT /note="Shows weak similarity to ML1410" FT /product="putative aspartate carbamoyltransferase" FT /tb_orthologue="pyrB" FT misc_feature 645590..646183 FT /colour=0 FT /note="Pfam match to entry PF00185 OTCace, FT Aspartate/ornithine carbamoyltransferase, score 173.40, FT E-value 3.7e-48" FT misc_feature 645731..645754 FT /colour=8 FT /note="PS00097 Aspartate and ornithine FT carbamoyltransferases signature" FT misc_feature 646328..646507 FT /colour=0 FT /note="Pfam match to entry PF00185 OTCace, FT Aspartate/ornithine carbamoyltransferase, score 31.40, FT E-value 8.4e-08" FT RBS 646530..646535 FT /note="possible RBS" FT CDS 646543..647835 FT /EC_number="3.5.2.3" FT /class="I.F.2" FT /colour=7 FT /gene="pyrC" FT /gene="ML0533" FT /note="Similar to M. tuberculosis pyrC, Rv1381, FT dihydroorotase, TR:P71809 (EMBL:AL123456) (430 aa); Fasta FT score E(): 0, 88.8% identity in 427 aa overlap. Similar to FT many e.g. Bacillus caldolyticus pyrC, dihydroorotase, FT SW:PYRC_BACCL (P46538) (427 aa); Fasta score E(): 0, 40.0% FT identity in 407 aa overlap. Contains Pfam match to entry FT PF00744 Dihydrooratase, Dihydroorotase-like." FT /product="putative dihydroorotase" FT /tb_orthologue="pyrC" FT misc_feature 646660..647808 FT /colour=0 FT /note="Pfam match to entry PF00744 Dihydrooratase, FT Dihydroorotase-like, score 266.90, E-value 2.7e-76" FT CDS 647832..648321 FT /class="V" FT /colour=0 FT /gene="ML0534" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1382 (Best blastx score 331)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT RBS 648407..648413 FT /note="possible RBS" FT CDS 648422..649549 FT /EC_number="6.3.5.5" FT /class="I.F.2" FT /colour=7 FT /gene="carA" FT /gene="ML0535" FT /note="Similar to M. tuberculosis carA, Rv1383, FT carbamoyl-phosphate synthase subunit, TR:P71811 FT (EMBL:AL123456) (376 aa); Fasta score E(): 0, 85.4% FT identity in 377 aa overlap. Similar to many e.g. FT Escherichia coli carA, carbamoyl-phosphate synthase FT subunit, SW:CARA_ECOLI (P00907) (382 aa); Fasta score E(): FT 0, 43.2% identity in 389 aa overlap. Contains Pfam match FT to entry PF00988 CPSase_sm_chain, Carbamoyl-phosphate FT synthase small chain, CPSase domain. Contains Pfam match FT to entry PF00117 GATase, Glutamine amidotransferase FT class-I. Contains PS00442 Glutamine amidotransferases FT class-I active site." FT /product="putative carbamoyl-phosphate synthase subunit" FT /tb_orthologue="carA" FT misc_feature 648431..648928 FT /colour=0 FT /note="Pfam match to entry PF00988 CPSase_sm_chain, FT Carbamoyl-phosphate synthase small chain, CPSase domain, FT score 282.20, E-value 6.7e-81" FT misc_feature 648977..649531 FT /colour=0 FT /note="Pfam match to entry PF00117 GATase, Glutamine FT amidotransferase class-I, score 185.60, E-value 7.8e-52" FT misc_feature 649184..649219 FT /colour=8 FT /note="PS00442 Glutamine amidotransferases class-I active FT site" FT RBS 649596..649603 FT /note="possible RBS" FT CDS 649608..652997 FT /EC_number="6.3.5.5" FT /class="I.F.2" FT /colour=7 FT /gene="carB" FT /gene="ML0536" FT /note="Similar to M. tuberculosis carB, Rv1384, FT carbamoyl-phosphate synthase subunit. Similar to many e.g. FT Escherichia coli carB, carbamoyl-phosphate synthase FT subunit, SW:CARB_ECOLI (P00968) (1072 aa); Fasta score FT E(): 0, 51.4% identity in 1116 aa overlap. Contains 2 Pfam FT matches to entry PF00289 CPSase_L_chain, FT Carbamoyl-phosphate synthase (CPSase). Contains 2 x FT PS00867 Carbamoyl-phosphate synthase subdomain signature FT 2. Contains PS00013 Prokaryotic membrane lipoprotein lipid FT attachment site. Contains PS00866 Carbamoyl-phosphate FT synthase subdomain signature 1." FT /product="putative carbamoyl-phosphate synthase subunit" FT /tb_orthologue="carB" FT misc_feature 649656..650864 FT /colour=0 FT /note="Pfam match to entry PF00289 CPSase_L_chain, FT Carbamoyl-phosphate synthase (CPSase), score 551.50, FT E-value 5.7e-162" FT misc_feature 649671..649703 FT /colour=8 FT /note="PS00013 Prokaryotic membrane lipoprotein lipid FT attachment site" FT misc_feature 650535..650558 FT /colour=8 FT /note="PS00867 Carbamoyl-phosphate synthase subdomain FT signature 2" FT misc_feature 651330..652307 FT /colour=0 FT /note="Pfam match to entry PF00289 CPSase_L_chain, FT Carbamoyl-phosphate synthase (CPSase), score 188.30, FT E-value 5.9e-53" FT misc_feature 651801..651845 FT /colour=8 FT /note="PS00866 Carbamoyl-phosphate synthase subdomain FT signature 1" FT misc_feature 652188..652211 FT /colour=8 FT /note="PS00867 Carbamoyl-phosphate synthase subdomain FT signature 2" FT RBS 652986..652990 FT /note="possible RBS" FT CDS 652994..653842 FT /EC_number="4.1.1.23" FT /class="I.F.2" FT /colour=7 FT /gene="pyrF" FT /gene="ML0537" FT /note="Similar to M. tuberculosis pyrF, Rv1385, orotidine FT 5'-phosphate decarboxylase, SW:DCOP_MYCTU (P77898) (274 FT aa); Fasta score E(): 0, 77.3% identity in 282 aa overlap. FT Similar to many e.g. Mycobacterium smegmatis pyrF, FT orotidine 5'-phosphate decarboxylase, SW:DCOP_MYCSM FT (O08323) (276 aa); Fasta score E(): 0, 72.5% identity in FT 280 aa overlap. Contains Pfam match to entry PF00215 FT OMPdecase, Orotidine 5'-phosphate decarboxylases. Contains FT PS00156 Orotidine 5'-phosphate decarboxylase active site." FT /product="orotidine 5'-phosphate decarboxylase" FT /tb_orthologue="pyrF" FT misc_feature 653003..653839 FT /colour=0 FT /note="Pfam match to entry PF00215 OMPdecase, Orotidine FT 5'-phosphate decarboxylases, score 188.80, E-value FT 9.5e-54" FT misc_feature 653261..653302 FT /colour=8 FT /note="PS00156 Orotidine 5'-phosphate decarboxylase active FT site" FT RBS 654117..654120 FT /note="possible RBS" FT CDS 654129..654437 FT /class="IV.C.1.a" FT /colour=6 FT /gene="PE" FT /gene="ML0538" FT /note="Similar to M. tuberculosis PE, Rv1386, PE-family FT protein, SW:YD86_MYCTU (P71656) (102 aa); Fasta score E(): FT 2.8e-25, 72.5% identity in 102 aa overlap." FT /note="Similar to ML452, ML1183 and ML2129" FT /product="PE-family protein" FT /tb_orthologue="PE" FT CDS 654434..656050 FT /class="IV.C.2" FT /colour=6 FT /gene="PPE" FT /gene="ML0539" FT /note="Similar to M. tuberculosis PPE, Rv1387, PPE-family FT protein, TR:P71657 (EMBL:AL123456) (539 aa); Fasta score FT E(): 0, 69.5% identity in 544 aa overlap. Contains Pfam FT match to entry PF00823 PPE, PPE family." FT /note="Similar to ML0411, ML1182, ML1828 and ML1991" FT /product="PPE-family protein" FT /tb_orthologue="PPE" FT misc_feature 654452..654928 FT /colour=0 FT /note="Pfam match to entry PF00823 PPE, PPE family, score FT 202.80, E-value 5.3e-57" FT RBS 656886..656890 FT /note="possible RBS" FT CDS 656895..657212 FT /class="II.A.5" FT /colour=2 FT /gene="mihF" FT /gene="ML0540" FT /note="Similar to M. tuberculosis mihF, Rv1388, possible FT integration host factor, TR:P71658 (EMBL:AL123456) (190 FT aa); Fasta score E(): 0, 98.1% identity in 105 aa overlap. FT Similar to Mycobacterium smegmatis mihF, integration host FT factor, TR:P96802 (EMBL:U75344) (105 aa); Fasta score E(): FT 7.5e-32, 94.1% identity in 102 aa overlap." FT /product="putative integration host factor" FT /tb_orthologue="mihF" FT RBS 657248..657252 FT /note="possible RBS" FT CDS 657266..657898 FT /EC_number="2.7.4.8" FT /class="I.F.1" FT /colour=7 FT /gene="gmk" FT /gene="ML0541" FT /note="Similar to M. tuberculosis gmk, Rv1389, putative FT guanylate kinase, SW:KGUA_MYCTU (P71659) (208 aa); Fasta FT score E(): 0, 79.1% identity in 201 aa overlap. Similar to FT many e.g. Saccharomyces cerevisiae guk1, guanylate kinase, FT SW:KGUA_YEAST (P15454) (186 aa); Fasta score E(): 7.1e-22, FT 42.8% identity in 180 aa overlap. Contains Pfam match to FT entry PF00625 Guanylate_kin, Guanylate kinase. Contains FT PS00017 ATP/GTP-binding site motif A (P-loop). Contains FT PS00856 Guanylate kinase signature." FT /product="putative guanylate kinase" FT /tb_orthologue="gmk" FT misc_feature 657353..657376 FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT misc_feature 657434..657487 FT /colour=8 FT /note="PS00856 Guanylate kinase signature" FT misc_feature 657437..657757 FT /colour=0 FT /note="Pfam match to entry PF00625 Guanylate_kin, FT Guanylate kinase, score 104.00, E-value 3e-27" FT RBS 657965..657971 FT /note="possible RBS" FT CDS 657980..658312 FT /class="V" FT /colour=10 FT /gene="ML0542" FT /note="Similar to M. tuberculosis Rv1390, conserved FT hypothetical protein, SW:YD90_MYCTU (P71660) (110 aa); FT Fasta score E(): 0, 90.0% identity in 110 aa overlap. Also FT similar to Streptomyces coelicolor SC9C5.02C, hypothetical FT protein, TR:CAB93358 (EMBL:AL357523) (90 aa); Fasta score FT E(): 2e-18, 71.3% identity in 80 aa overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv1390" FT RBS 658326..658332 FT /note="possible RBS" FT CDS 658333..659592 FT /class="I.B.7" FT /colour=7 FT /gene="dfp" FT /gene="ML0543" FT /note="Similar to M. tuberculosis dfp, Rv1391, putative FT flavoprotein, SW:DFP_MYCTU (P71661) (418 aa); Fasta score FT E(): 0, 87.0% identity in 409 aa overlap. Similar to many FT e.g. Escherichia coli dfp, DNA/pantothenate metabolism FT flavoprotein, SW:DFP_ECOLI (P24285) (430 aa); Fasta score FT E(): 0, 39.7% identity in 408 aa overlap." FT /product="putative flavoprotein" FT /tb_orthologue="dfp" FT RBS 659704..659709 FT /note="possible RBS" FT CDS 659721..660932 FT /EC_number="2.5.1.6" FT /class="I.D.2" FT /colour=7 FT /gene="metK" FT /gene="ML0544" FT /note="Similar to M. tuberculosis metK, Rv1392, putative FT S-adenosylmethionine synthase, SW:METK_MYCTU (P77899) (403 FT aa); Fasta score E(): 0, 91.3% identity in 403 aa overlap. FT Similar to Streptomyces spectabilis metK, FT S-adenosylmethionine synthase, SW:METK_STRST (Q9X4Q2) (411 FT aa); Fasta score E(): 0, 73.6% identity in 398 aa overlap. FT Contains Pfam match to entry PF00438 S-AdoMet_synt, FT S-adenosylmethionine synthetase. Contains PS00377 FT S-adenosylmethionine synthetase signature 2. Contains FT PS00376 S-adenosylmethionine synthetase signature 1." FT /product="putative S-adenosylmethionine synthase" FT /tb_orthologue="metK" FT misc_feature 659736..660893 FT /colour=0 FT /note="Pfam match to entry PF00438 S-AdoMet_synt, FT S-adenosylmethionine synthetase, score 693.20, E-value FT 3.2e-287" FT misc_feature 660111..660143 FT /colour=8 FT /note="PS00376 S-adenosylmethionine synthetase signature FT 1" FT misc_feature 660555..660581 FT /colour=8 FT /note="PS00377 S-adenosylmethionine synthetase signature FT 2" FT CDS complement(661595..662509) FT /class="II.B.5" FT /colour=0 FT /gene="lipH" FT /gene="ML0545" FT /note="Possible pseudogene of M. tuberculosis orthologue FT lipH (Best blastx score 213)" FT /product="probable lipase (pseudogene)" FT /pseudo FT CDS complement(662674..663497) FT /class="II.B.5" FT /colour=0 FT /gene="lipI" FT /gene="ML0546" FT /note="Possible pseudogene of M. tuberculosis orthologue FT lipI (Best blastx score 262)" FT /product="probable lipase (pseudogene)" FT /pseudo FT CDS 663496..663987 FT /class="II.C.4" FT /colour=0 FT /gene="ML0547" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1401 (Best blastx score 114)" FT /product="possible membrane protein (pseudogene)" FT /pseudo FT CDS 664260..666215 FT /class="II.A.5" FT /colour=2 FT /gene="priA" FT /gene="ML0548" FT /note="Similar to M. tuberculosis priA, Rv1402, putative FT primosomal protein N', SW:PRIA_MYCTU (P71670) (655 aa); FT Fasta score E(): 0, 80.7% identity in 658 aa overlap. FT Similar to many e.g. Escherichia coli priA, primosomal FT protein N', SW:PRIA_ECOLI (P17888) (732 aa); Fasta score FT E(): 1.2e-16, 28.1% identity in 445 aa overlap." FT /product="putative primosomal protein N'" FT /tb_orthologue="priA" FT CDS complement(666224..667066) FT /class="IV.H" FT /colour=0 FT /gene="ML0549" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1403c (Best blastx score 822)" FT /product="similar to phosphatidylethanolamine FT N-methyltransferase (pseudogene)" FT /pseudo FT CDS 667331..667816 FT /class="I.J.1" FT /colour=9 FT /gene="ML0550" FT /note="Similar to M. tuberculosis Rv1404, putative FT marR-family transcriptional regulator, TR:P71672 FT (EMBL:AL123456) (160 aa); Fasta score E(): 0, 79.1% FT identity in 158 aa overlap. Shows weak similarity to marR FT family e.g. Salmonella typhimurium marR, multiple FT antibiotic resistance protein, SW:MARR_SALTY (Q56069) (144 FT aa); Fasta score E(): 0.0027, 29.2% identity in 106 aa FT overlap. Contains Pfam match to entry PF01047 MarR, MarR FT family." FT /product="putative marR-family transcriptional regulator" FT /tb_orthologue="Rv1404" FT misc_feature 667454..667765 FT /colour=0 FT /note="Pfam match to entry PF01047 MarR, MarR family, FT score 99.80, E-value 5.2e-26" FT CDS complement(667998..668822) FT /class="IV.H" FT /colour=7 FT /gene="ML0551" FT /note="Similar to M. tuberculosis Rv1405c, hypothetical FT protein, SW:YE05_MYCTU (P71673) (274 aa); Fasta score E(): FT 0, 78.0% identity in 273 aa overlap. Shows weak similarity FT to methyltransferses e.g. Rhodobacter sphaeroides pmtA, FT phosphatidylethanolamine N-methyltransferase, FT SW:PMTA_RHOSH (Q05197) (203 aa); Fasta score E(): 7.9e-08, FT 31.3% identity in 144 aa overlap and Escherichia coli FT ubiE, ubiquinone/menaquinone biosynthesis FT methyltransferase, SW:UBIE_ECOLI (P27851) (251 aa); Fasta FT score E(): 9.1e-06, 28.0% identity in 118 aa overlap." FT /product="putative methyltransferase" FT /tb_orthologue="Rv1405c" FT RBS complement(668828..668832) FT /note="possible RBS" FT RBS 668985..668988 FT /note="possible RBS" FT CDS 669003..669959 FT /EC_number="2.1.2.9" FT /class="II.A.3" FT /colour=2 FT /gene="fmt" FT /gene="ML0552" FT /note="Similar to M. tuberculosis fmt, Rv1406, putative FT methionyl-tRNA formyltransferase, SW:FMT_MYCTU (P71674) FT (312 aa); Fasta score E(): 0, 81.0% identity in 316 aa FT overlap. Similar to many e.g. Thermus aquaticus fmt, FT methionyl-tRNA formyltransferase, SW:FMT_THETH (P43523) FT (305 aa); Fasta score E(): 8.1e-31, 41.3% identity in 315 FT aa overlap. Contains Pfam match to entry PF00551 FT formyl_transf, Formyl transferase." FT /product="putative methionyl-tRNA formyltransferase" FT /tb_orthologue="fmt" FT misc_feature 669009..669551 FT /colour=0 FT /note="Pfam match to entry PF00551 formyl_transf, Formyl FT transferase, score 105.10, E-value 3.9e-29" FT CDS 670028..671362 FT /class="II.A.4" FT /colour=0 FT /gene="fmu" FT /gene="ML0553" FT /note="Possible pseudogene of M. tuberculosis orthologue FT fmu (Best blastx score 1324)" FT /product="similar to Fmu protein (pseudogene)" FT /pseudo FT CDS 671416..672090 FT /EC_number="5.1.3.1" FT /class="I.B.5" FT /colour=7 FT /gene="rpe" FT /gene="ML0554" FT /note="Similar to M. tuberculosis rpe, Rv1408, FT ribulose-phosphate 3-epimerase, SW:RPE_MYCTU (P71676) (232 FT aa); Fasta score E(): 0, 91.0% identity in 221 aa overlap. FT Similar to many e.g. Oryza sativa D-ribulose-5-phosphate FT 3-epimerase, TR:Q9SE42 (EMBL:AF189365) (228 aa); Fasta FT score E(): 0, 47.0% identity in 215 aa overlap. Contains FT Pfam match to entry PF00834 Ribul_P_3_epim, FT Ribulose-phosphate 3 epimerase family. Contains PS01085 FT Ribulose-phosphate 3-epimerase family signature 1. FT Contains PS01086 Ribulose-phosphate 3-epimerase family FT signature 2." FT /product="putatibe ribulose-phosphate 3-epimerase" FT /tb_orthologue="rpe" FT misc_feature 671425..672021 FT /colour=0 FT /note="Pfam match to entry PF00834 Ribul_P_3_epim, FT Ribulose-phosphate 3 epimerase family, score 410.10, FT E-value 2e-119" FT misc_feature 671503..671547 FT /colour=8 FT /note="PS01085 Ribulose-phosphate 3-epimerase family FT signature 1" FT misc_feature 671809..671877 FT /colour=8 FT /note="PS01086 Ribulose-phosphate 3-epimerase family FT signature 2" FT CDS 672087..673106 FT /EC_number="3.5.4.26" FT /EC_number="1.1.1.193" FT /class="I.G.9" FT /colour=7 FT /gene="ribG" FT /gene="ML0555" FT /note="Similar to M. tuberculosis ribG, Rv1409, putative FT riboflavin biosynthesis protein, SW:RIBD_MYCTU (P71677) FT (339 aa); Fasta score E(): 0, 78.2% identity in 339 aa FT overlap. Similar to many e.g. Bacillus subtilis ribD, FT diaminohydroxyphosphoribosylaminopyrimidine deaminase / FT 5-amino-6-(5-phosphoribosylamino)-uracil reductase, FT SW:RIBD_BACSU (P17618) (361 aa); Fasta score E(): 7.9e-33, FT 39.2% identity in 357 aa overlap. Contains Pfam match to FT entry PF00383 dCMP_cyt_deam, Cytidine and deoxycytidylate FT deaminase zinc-binding region. Contains Pfam match to FT entry PF01872 RibD_C, RibD C-terminal domain. Contains FT PS00903 Cytidine and deoxycytidylate deaminases FT zinc-binding region signature." FT /product="putative bifunctional riboflavin-specific FT deaminase/reductase" FT /tb_orthologue="ribG" FT misc_feature 672162..672395 FT /colour=0 FT /note="Pfam match to entry PF00383 dCMP_cyt_deam, Cytidine FT and deoxycytidylate deaminase zinc-binding region, score FT 114.30, E-value 2.4e-30" FT misc_feature 672255..672371 FT /colour=8 FT /note="PS00903 Cytidine and deoxycytidylate deaminases FT zinc-binding region signature" FT misc_feature 672549..673058 FT /colour=0 FT /note="Pfam match to entry PF01872 RibD_C, RibD C-terminal FT domain, score 258.40, E-value 9.8e-74" FT CDS complement(673150..674679) FT /class="III.A.6" FT /colour=3 FT /gene="ML0556" FT /note="Similar to M. tuberculosis Rv1410c, probable drug FT resistance transporter, TR:P71678 (EMBL:AL123456) (518 FT aa); Fasta score E(): 0, 82.0% identity in 506 aa overlap. FT Similar to many e.g. Bacillus subtilis bmr3, multidrug FT transporter, TR:P96712 (EMBL:D50098) (512 aa); Fasta score FT E(): 5.4e-24, 27.1% identity in 446 aa overlap. Contains FT hydrophobic, probable membrane-spanning regions. Contains FT Pfam match to entry PF00083 sugar_tr, Sugar (and other) FT transporter. Contains PS00216 Sugar transport proteins FT signature 1." FT /note="Similar to ML1562" FT /product="putative integral membrane drug transport FT protein" FT /tb_orthologue="Rv1410c" FT misc_feature complement(673459..674640) FT /colour=0 FT /note="Pfam match to entry PF00083 sugar_tr, Sugar (and FT other) transporter, score -86.10, E-value 0.00086" FT misc_feature complement(674431..674481) FT /colour=8 FT /note="PS00216 Sugar transport proteins signature 1" FT CDS complement(674679..675395) FT /class="II.C.1" FT /colour=3 FT /gene="lprG" FT /gene="ML0557" FT /note="Similar to M. tuberculosis lprG, Rv1411c, putative FT lipoprotein, SW:LPRG_MYCTU (P71679) (236 aa); Fasta score FT E(): 0, 68.1% identity in 238 aa overlap and to other M. FT tuberculosis lipoproteins. 27 kDa antigen (Mycobacterium FT bovis). Contains probable N-terminal signal sequence. FT Contains PS00013 Prokaryotic membrane lipoprotein lipid FT attachment site." FT /note="Similar to ML0136" FT /product="putative lipoprotein" FT /tb_orthologue="lprG" FT misc_feature complement(675315..675347) FT /colour=8 FT /note="PS00013 Prokaryotic membrane lipoprotein lipid FT attachment site" FT CDS 675552..676175 FT /EC_number="2.5.1.9" FT /class="I.G.9" FT /colour=7 FT /gene="ribC" FT /gene="ML0558" FT /note="Similar to M. tuberculosis ribC, Rv1412, riboflavin FT synthase [alpha] chain, SW:RISA_MYCTU (P71680) (201 aa); FT Fasta score E(): 0, 82.4% identity in 199 aa overlap. FT Similar to many e.g. Bacillus subtilis ribE, riboflavin FT synthase alpha chain, SW:RISA_BACSU (P16440) (215 aa); FT Fasta score E(): 9.5e-28, 43.4% identity in 198 aa FT overlap. Contains 2 Pfam matches to entry PF00677 FT Lum_binding, Lumazine binding domain. Contains 2 x PS00693 FT Riboflavin synthase alpha chain family signature." FT /product="putative riboflavin synthase [alpha] chain" FT /tb_orthologue="ribC" FT misc_feature 675585..675812 FT /colour=0 FT /note="Pfam match to entry PF00677 Lum_binding, Lumazine FT binding domain, score 59.90, E-value 5.6e-14" FT misc_feature 675768..675806 FT /colour=8 FT /note="PS00693 Riboflavin synthase alpha chain family FT signature" FT misc_feature 675849..676112 FT /colour=0 FT /note="Pfam match to entry PF00677 Lum_binding, Lumazine FT binding domain, score 77.10, E-value 3.7e-19" FT misc_feature 676068..676106 FT /colour=8 FT /note="PS00693 Riboflavin synthase alpha chain family FT signature" FT CDS 676302..677609 FT /EC_number="3.5.4.25" FT /class="I.G.9" FT /colour=7 FT /gene="ribA" FT /gene="ML0559" FT /note="Similar to M. tuberculosis ribA2, Rv1415, putative FT GTP cyclohydrolase II, SW:GCH2_MYCTU (P71684) (425 aa); FT Fasta score E(): 0, 93.4% identity in 425 aa overlap. FT Similar to many e.g. Actinobacillus pleuropneumoniae ribA, FT riboflavin biosynthesis protein, SW:GCH2_ACTPL (P50855) FT (401 aa); Fasta score E(): 0, 52.4% identity in 401 aa FT overlap. Contains Pfam match to entry PF00926 FT DHBP_synthase, 3,4-dihydroxy-2-butanone 4-phosphate FT synthase. Contains Pfam match to entry PF00925 FT GTP_cyclohydro2, GTP cyclohydrolase II." FT /product="putative GTP cyclohydrolase FT II/3,4-dihydroxy-2-butanone-4-phosphate synthase" FT /tb_orthologue="ribA2" FT misc_feature 676350..676943 FT /colour=0 FT /note="Pfam match to entry PF00926 DHBP_synthase, FT 3,4-dihydroxy-2-butanone 4-phosphate synthase, score FT 455.00, E-value 6.3e-133" FT misc_feature 676950..677477 FT /colour=0 FT /note="Pfam match to entry PF00925 GTP_cyclohydro2, GTP FT cyclohydrolase II, score 402.80, E-value 3.3e-117" FT CDS 677606..678088 FT /EC_number="2.5.1.9" FT /class="I.G.9" FT /colour=7 FT /gene="ribH" FT /gene="ML0560" FT /note="Similar to M. tuberculosis ribH, Rv1416, riboflavin FT synthase [beta] chain, SW:RISB_MYCTU (P71685) (154 aa); FT Fasta score E(): 0, 78.9% identity in 152 aa overlap. FT Similar to many e.g. Actinobacillus pleuropneumoniae ribH, FT 6,7-dimethyl-8-ribityllumazine synthase, SW:RISB_ACTPL FT (P50856) (153 aa); Fasta score E(): 1.7e-15, 44.3% FT identity in 140 aa overlap. Contains Pfam match to entry FT PF00885 DMRL_synthase, 6,7-dimethyl-8-ribityllumazine FT synthase." FT /product="6,7-dimethyl-8-ribityllumazine synthase" FT /tb_orthologue="ribH" FT misc_feature 677648..678073 FT /colour=0 FT /note="Pfam match to entry PF00885 DMRL_synthase, FT 6,7-dimethyl-8-ribityllumazine synthase, score 250.90, FT E-value 1.7e-71" FT CDS 678085..678555 FT /class="II.C.4" FT /colour=3 FT /gene="ML0561" FT /note="Similar to M. tuberculosis Rv1417, hypothetical FT protein, SW:YE17_MYCTU (P71686) (154 aa); Fasta score E(): FT 0, 75.5% identity in 143 aa overlap. Similar to other FT hypothetical proteins e.g. Corynebacterium ammoniagenes FT ribX, hypothetical protein, TR:O24754 (EMBL:AB003693) (184 FT aa); Fasta score E(): 2.1e-15, 34.5% identity in 148 aa FT overlap. Contains hydrophobic, possible membrane-spanning FT regions." FT /product="putative membrane protein" FT /tb_orthologue="Rv1417" FT CDS 678870..680813 FT /class="II.A.5" FT /colour=2 FT /gene="uvrC" FT /gene="ML0562" FT /note="Similar to M. tuberculosis uvrC, Rv1420, FT excinuclease ABC subunit C, SW:UVRC_MYCTU (P71689) (646 FT aa); Fasta score E(): 0, 87.6% identity in 646 aa overlap. FT Similar to many e.g. Escherichia coli uvrC, excinuclease FT ABC subunit C, SW:UVRC_ECOLI (P07028) (231 aa); BlastP FT Expect 8.9. Contains Pfam match to entry PF01541 FT Exci_endo_N, Endo/excinuclease amino terminal domain. FT Contains Pfam match to entry PF02151 UVR, UvrB/uvrC motif. FT Contains Pfam match to entry PF00633 HHH, FT Helix-hairpin-helix motif.." FT /product="putative excinuclease ABC subunit C" FT /tb_orthologue="uvrC" FT misc_feature 678918..679190 FT /colour=0 FT /note="Pfam match to entry PF01541 Exci_endo_N, FT Endo/excinuclease amino terminal domain, score 55.10, FT E-value 1.5e-12" FT misc_feature 679491..679598 FT /colour=0 FT /note="Pfam match to entry PF02151 UVR, UvrB/uvrC motif, FT score 42.80, E-value 7.7e-09" FT misc_feature 680691..680780 FT /colour=0 FT /note="Pfam match to entry PF00633 HHH, FT Helix-hairpin-helix motif., score 28.10, E-value 0.00021" FT CDS 680810..681706 FT /class="V" FT /colour=10 FT /gene="ML0563" FT /note="Similar to M. tuberculosis Rv1421, conserved FT hypothetical protein, SW:YE21_MYCTU (P71690) (301 aa); FT Fasta score E(): 0, 92.3% identity in 285 aa overlap. FT Conserved in many bacteria." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv1421" FT CDS 681703..682782 FT /class="V" FT /colour=10 FT /gene="ML0564" FT /note="Similar to M. tuberculosis Rv1422, conserved FT hypothetical protein, SW:YE22_MYCTU (P71691) (342 aa); FT Fasta score E(): 0, 85.7% identity in 350 aa overlap. FT Conserved in many bacteria." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv1422" FT CDS 682779..683756 FT /class="I.J.1" FT /colour=9 FT /gene="ML0565" FT /note="Similar to M. tuberculosis Rv1423, putative FT transcriptional regulator, TR:P71692 (EMBL:AL123456) (325 FT aa); Fasta score E(): 0, 92.0% identity in 325 aa overlap. FT Similar to Streptomyces coelicolor whiA, sporulation FT regulatory protein, TR:Q9S4Y1 (EMBL:AF106003) (330 aa); FT Fasta score E(): 0, 67.6% identity in 324 aa overlap." FT /product="putative transcriptional regulator" FT /tb_orthologue="Rv1423" FT CDS 683866..684275 FT /class="V" FT /colour=0 FT /gene="ML0566" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1425 (Best blastx score 264)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS 684845..686398 FT /class="II.C.4" FT /colour=0 FT /gene="ML0567" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1431 (Best blastx score 550)" FT /product="possible transporter (pseudogene)" FT /pseudo FT RBS 686803..686806 FT /note="possible RBS" FT CDS 686813..687406 FT /class="V" FT /colour=10 FT /gene="ML0568" FT /note="Similar to M. tuberculosis Rv1435c, hypothetical FT protein, TR:O06823 (EMBL:AL123456) (202 aa); Fasta score FT E(): 1.7e-07, 38.9% identity in 203 aa overlap. Pro- and FT Val-rich protein." FT /product="hypothetical protein" FT repeat_region 690412..691732 FT CDS complement(693611..694426) FT /class="II.C.4" FT /colour=3 FT /gene="ML0569" FT /note="Similar to M. tuberculosis Rv1433, putative FT membrane protein, TR:O06825 (EMBL:AL123456) (271 aa); FT Fasta score E(): 0, 68.3% identity in 271 aa overlap. FT Shows weak similarity to other hypothetical proteins in M. FT tuberculosis. Previously sequenced as TR:Q49706 FT (EMBL:U00013) (271 aa); Fasta score E(): 0, 100.0% FT identity in 271 aa overlap." FT /note="Similar to ML0426, ML2446 and ML2664" FT /product="putative membrane protein" FT /tb_orthologue="Rv1433" FT RBS 694876..694882 FT /note="possible RBS" FT CDS 694889..695908 FT /EC_number="1.2.1.12" FT /class="I.B.1" FT /colour=7 FT /gene="gap" FT /gene="ML0570" FT /note="Similar to M. tuberculosis gap, Rv1436, FT glyceraldehyde 3-phosphate dehydrogenase, SW:G3P_MYCTU FT (O06822) (339 aa); Fasta score E(): 0, 89.1% identity in FT 339 aa overlap. Similar to many e.g. Mycobacterium avium FT gap, glyceraldehyde 3-phosphate dehydrogenase, FT SW:G3P_MYCAV (P94915) (339 aa); Fasta score E(): 0, 87.9% FT identity in 339 aa overlap. Previously sequenced as FT SW:G3P_MYCLE (P46713) (339 aa); Fasta score E(): 0, 99.7% FT identity in 339 aa overlap. Contains Pfam match to entry FT PF00044 gpdh, glyceraldehyde 3-phosphate dehydrogenases. FT Contains PS00071 Glyceraldehyde 3-phosphate dehydrogenase FT active site." FT /product="glyceraldehyde 3-phosphate dehydrogenase" FT /tb_orthologue="gap" FT misc_feature 694892..695902 FT /colour=0 FT /note="Pfam match to entry PF00044 gpdh, glyceraldehyde FT 3-phosphate dehydrogenases, score 733.80, E-value FT 7.9e-219" FT misc_feature 695354..695377 FT /colour=8 FT /note="PS00071 Glyceraldehyde 3-phosphate dehydrogenase FT active site" FT misc_feature 695911..697140 FT /colour=0 FT /note="Pfam match to entry PF00162 PGK, Phosphoglycerate FT kinases, score 757.00, E-value 7.9e-224" FT CDS 695911..697161 FT /EC_number="2.7.2.3" FT /class="I.B.1" FT /colour=7 FT /gene="pgk" FT /gene="ML0571" FT /note="Similar to M. tuberculosis pgk, Rv1437, FT phosphoglycerate kinase, SW:PGK_MYCTU (O06821) (412 aa); FT Fasta score E(): 0, 80.6% identity in 412 aa overlap. FT Similar to many e.g. Corynebacterium glutamicum pgk, FT phosphoglycerate kinase, SW:PGK_CORGL (Q01655) (403 aa); FT Fasta score E(): 0, 59.2% identity in 407 aa overlap. FT Previously sequenced as SW:PGK_MYCLE (P46712) (416 aa); FT Fasta score E(): 0, 99.8% identity in 416 aa overlap. FT Contains Pfam match to entry PF00162 PGK, Phosphoglycerate FT kinases. Contains PS00111 Phosphoglycerate kinase FT signature." FT /product="phosphoglycerate kinase" FT /tb_orthologue="pgk" FT misc_feature 695962..695994 FT /colour=8 FT /note="PS00111 Phosphoglycerate kinase signature" FT RBS 697149..697153 FT /note="possible RBS" FT CDS 697158..697943 FT /EC_number="5.3.1.1" FT /class="I.B.1" FT /colour=7 FT /gene="tpi" FT /gene="ML0572" FT /note="Similar to M. tuberculosis tpi, Rv1438, FT triosephosphate isomerase, SW:TPIS_MYCTU (O08408) (261 FT aa); Fasta score E(): 0, 83.9% identity in 261 aa overlap. FT Similar to many e.g. Corynebacterium glutamicum tpi, FT triosephosphate isomerase, SW:TPIS_CORGL (P19583) (259 FT aa); Fasta score E(): 0, 64.5% identity in 256 aa overlap. FT Previously sequenced as SW:TPIS_MYCLE (P46711) (261 aa); FT Fasta score E(): 0, 99.6% identity in 261 aa overlap. FT Contains Pfam match to entry PF00121 TIM, Triosephosphate FT isomerase. Contains PS00171 Triosephosphate isomerase FT active site." FT /product="triosephosphate isomerase" FT /tb_orthologue="tpi" FT misc_feature 697164..697931 FT /colour=0 FT /note="Pfam match to entry PF00121 TIM, Triosephosphate FT isomerase, score 524.60, E-value 3.7e-169" FT misc_feature 697665..697697 FT /colour=8 FT /note="PS00171 Triosephosphate isomerase active site" FT CDS 697927..698202 FT /class="VI" FT /colour=8 FT /gene="ML0573" FT /ml_unique FT /note="Unknown function. Previously sequenced as TR:Q49684 FT (EMBL:U00013) (91 aa); Fasta score E(): 0, 98.9% identity FT in 91 aa overlap. Contains Pfam match to entry PF00121 FT TIM, Triosephosphate isomerase." FT /product="hypothetical protein" FT CDS 698199..698516 FT /class="VI" FT /colour=8 FT /gene="ML0574" FT /ml_unique FT /note="Unknown function. Previously sequenced as TR:Q49680 FT (EMBL:U00013) (105 aa); Fasta score E(): 0, 99.0% identity FT in 105 aa overlap." FT /product="hypothetical protein" FT CDS 698674..698892 FT /class="VI" FT /colour=8 FT /gene="ML0575" FT /ml_unique FT /note="Unknown function." FT /product="hypothetical protein" FT CDS 699708..699938 FT /class="VI" FT /colour=8 FT /gene="ML0576" FT /ml_unique FT /note="Unknown function. Previously sequenced as TR:Q49681 FT (EMBL:U00013) (76 aa); Fasta score E(): 1.5e-31, 100.0% FT identity in 76 aa overlap." FT /product="hypothetical protein" FT CDS 699950..700183 FT /class="III.D" FT /colour=3 FT /gene="ML0577" FT /note="Similar to M. tuberculosis secG, Rv1440, probable FT protein-export membrane protein, SW:SECG_MYCTU (O06819) FT (77 aa); Fasta score E(): 1.1e-25, 96.1% identity in 77 aa FT overlap. Similar to many e.g. Escherichia coli secG, FT protein-export membrane protein, SW:SECG_ECOLI (P33582) FT (110 aa); Fasta score E(): 0.06, 29.0% identity in 69 aa FT overlap. Previously sequenced as SW:SECG_MYCLE (P38388) FT (77 aa); Fasta score E(): 3.1e-26, 100.0% identity in 77 FT aa overlap. Contains hydrophobic, possible FT membrane-spanning regions." FT /product="putative protein-export membrane protein" FT CDS 700214..703018 FT /EC_number="4.1.1.31" FT /class="I.C.1" FT /colour=7 FT /gene="ppc" FT /gene="ML0578" FT /ml_unique FT /note="Similar to many e.g. Rhodopseudomonas palustris FT ppc, phosphoenolpyruvate carboxylase, SW:CAPP_RHOPA FT (O32483) (936 aa); Fasta score E(): 0, 45.7% identity in FT 936 aa overlap. Previously sequenced as SW:CAPP_MYCLE FT (P46710) (907 aa); Fasta score E(): 0, 100.0% identity in FT 907 aa overlap. Contains 2 Pfam matches to entry PF00311 FT PEPcase, Phosphoenolpyruvate carboxylase. Contains PS00781 FT Phosphoenolpyruvate carboxylase active site 1. Contains FT PS00393 Phosphoenolpyruvate carboxylase active site 2." FT /product="putative phosphoenolpyruvate carboxylase" FT misc_feature 700454..701158 FT /colour=0 FT /note="Pfam match to entry PF00311 PEPcase, FT Phosphoenolpyruvate carboxylase, score 49.50, E-value FT 1.2e-14" FT misc_feature 700682..700717 FT /colour=8 FT /note="PS00781 Phosphoenolpyruvate carboxylase active site FT 1" FT misc_feature 701390..702934 FT /colour=0 FT /note="Pfam match to entry PF00311 PEPcase, FT Phosphoenolpyruvate carboxylase, score 347.00, E-value FT 1.2e-103" FT misc_feature 701963..702001 FT /colour=8 FT /note="PS00393 Phosphoenolpyruvate carboxylase active site FT 2" FT CDS complement(703242..703985) FT /EC_number="3.1.1.31" FT /class="I.B.5" FT /colour=7 FT /gene="pgl" FT /gene="ML0579" FT /note="Similar to M. tuberculosis devB, Rv1445c, FT 6-phosphogluconolactonase, SW:6PGL_MYCTU (O06814) (247 FT aa); Fasta score E(): 0, 72.8% identity in 246 aa overlap. FT Similar to many e.g. C-terminal half of Homo sapiens FT gdh/6pgl endoplasmic bifunctional protein precursor FT (6-phosphogluconolactonase), SW:. Previously sequenced as FT SW:6PGL_MYCLE (Q49700) (247 aa); Fasta score E(): 0, 99.6% FT identity in 247 aa overlap. Contains Pfam match to entry FT PF01182 Glucosamine_iso, Glucosamine-6-phosphate FT isomerase." FT /product="putative 6-phosphogluconolactonase" FT /tb_orthologue="devB" FT misc_feature complement(703269..703913) FT /colour=0 FT /note="Pfam match to entry PF01182 Glucosamine_iso, FT Glucosamine-6-phosphate isomerase, score 4.50, E-value FT 1.4e-06" FT CDS complement(704001..704798) FT /class="V" FT /colour=10 FT /gene="ML0580" FT /note="Similar to M. tuberculosis Rv1446c, hypothetical FT protein, TR:O06813 (EMBL:AL123456) (303 aa); Fasta score FT E(): 0, 70.3% identity in 239 aa overlap. Similar to FT hypothetical proteins from Streptomyces coelicolor e.g. FT SCC22.20, hypothetical protein, TR:Q9XAB8 (EMBL:AL096839) FT (351 aa); Fasta score E(): 7.1e-21, 36.0% identity in 203 FT aa overlap, although these have a short N-terminal FT extension relative to this homologue. Previously sequencd FT as TR:Q49699 (EMBL:U00013) (265 aa); Fasta score E(): 0, FT 100.0% identity in 265 aa overlap." FT /product="conserved hypothetical protein" FT RBS complement(704813..704818) FT /note="possible RBS" FT CDS complement(704918..706434) FT /class="I.B.5" FT /colour=0 FT /gene="zwf2" FT /gene="ML0581" FT /note="Possible pseudogene of M. tuberculosis orthologue FT zwf2 (Best blastx score 638)" FT /product="glucose-6-phosphate 1-dehydrogenase FT (pseudogene)" FT /pseudo FT CDS complement(706461..707588) FT /EC_number="2.2.1.2" FT /class="I.B.5" FT /colour=7 FT /gene="tal" FT /gene="ML0582" FT /note="Similar to M. tuberculosis tal, Rv1448c, FT transaldolase, SW:TAL_MYCTU (O06812) (373 aa); Fasta score FT E(): 0, 78.9% identity in 370 aa overlap. Similar to many FT e.g. Escherichia coli tal, transaldolase, SW:TALA_ECOLI FT (P78258) (316 aa); Fasta score E(): 7e-05, 29.6% identity FT in 270 aa overlap. Previously sequenced as SW:TAL_MYCLE FT (P55193) (375 aa); Fasta score E(): 0, 100.0% identity in FT 375 aa overlap. Contains Pfam match to entry PF00923 FT Transaldolase, Transaldolase. Contains PS01054 FT Transaldolase signature 1. Contains PS00958 Transaldolase FT active site." FT /product="putative transaldolase" FT /tb_orthologue="tal" FT misc_feature complement(706746..707522) FT /colour=0 FT /note="Pfam match to entry PF00923 Transaldolase, FT Transaldolase, score 413.80, E-value 1.6e-120" FT misc_feature complement(707112..707165) FT /colour=8 FT /note="PS00958 Transaldolase active site" FT misc_feature complement(707427..707453) FT /colour=8 FT /note="PS01054 Transaldolase signature 1" FT CDS complement(707605..709704) FT /EC_number="2.2.1.1" FT /class="I.B.5" FT /colour=7 FT /gene="tkt" FT /gene="ML0583" FT /note="Similar to M. tuberculosis tkt, Rv1449c, FT transketolase, SW:TKT_MYCTU (O06811) (700 aa); Fasta score FT E(): 0, 89.1% identity in 700 aa overlap. Similar to many FT e.g. Saccharomyces cerevisiae tkl2, transketolase 2, FT SW:TKT2_YEAST (P33315) (681 aa); Fasta score E(): 0, 42.8% FT identity in 684 aa overlap. Previously sequenced as FT SW:TKT_MYCLE (P46708) (699 aa); Fasta score E(): 0, 99.9% FT identity in 699 aa overlap. Contains Pfam match to entry FT PF00456 transketolase, Transketolase. Contains PS00801 FT Transketolase signature 1. Contains PS00802 Transketolase FT signature 2." FT /product="transketolase" FT /tb_orthologue="tkt" FT misc_feature complement(707638..709611) FT /colour=0 FT /note="Pfam match to entry PF00456 transketolase, FT Transketolase, score 1459.70, E-value 0" FT misc_feature complement(708166..708216) FT /colour=8 FT /note="PS00802 Transketolase signature 2" FT misc_feature complement(709552..709614) FT /colour=8 FT /note="PS00801 Transketolase signature 1" FT RBS complement(709712..709716) FT /note="possible RBS" FT CDS 709904..710869 FT /EC_number="2.5.1.-" FT /class="I.B.6.a" FT /colour=7 FT /gene="ctaB" FT /gene="ML0584" FT /note="Similar to M. tuberculosis ctaB, Rv1451, cytochrome FT c oxidase assembly factor, TR:O06809 (EMBL:AL123456) (308 FT aa); Fasta score E(): 0, 82.7% identity in 307 aa overlap. FT Similar to many e.g. Bacillus firmus ctaB, protoheme IX FT farnesyltransferase, SW:COXX_BACFI (Q04444) (312 aa); FT Fasta score E(): 7.8e-24, 33.6% identity in 283 aa FT overlap. Previously sequenced as TR:Q49685 (EMBL:U00013) FT (300 aa); Fasta score E(): 0, 99.7% identity in 300 aa FT overlap. Contains hydrophobic, possible membrane-spanning FT regions. Contains Pfam match to entry PF01040 UbiA, UbiA FT prenyltransferase family." FT /product="putative protoheme IX farnesyltransferase" FT /tb_orthologue="ctaB" FT misc_feature 710036..710845 FT /colour=0 FT /note="Pfam match to entry PF01040 UbiA, UbiA FT prenyltransferase family, score 360.30, E-value 2e-104" FT CDS complement(710904..711810) FT /class="I.B.7" FT /colour=0 FT /gene="qor" FT /gene="ML0585" FT /note="Possible pseudogene of M. tuberculosis orthologue FT qor (Best blastx score 228)" FT /product="Probable quinone oxidoreductase (pseudogene)" FT /pseudo FT CDS 711832..712694 FT /class="V" FT /colour=0 FT /gene="ML0586" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1455 (Best blastx score 414)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(712668..713603) FT /class="II.C.4" FT /colour=3 FT /gene="ML0587" FT /note="Similar to M. tuberculosis Rv1456c, putative FT membrane protein, TR:O53148 (EMBL:AL123456) (310 aa); FT Fasta score E(): 0, 83.3% identity in 300 aa overlap. FT Similar to bacterial hypothetical proteins e.g. FT Mycobacterium smegmatis fxtF, hypothetical protein, FT TR:O87318 (EMBL:AF027770) (236 aa); Fasta score E(): 0, FT 67.5% identity in 228 aa overlap. Previously sequenced as FT TR:O33142 (EMBL:Z99125) (311 aa); Fasta score E(): 0, FT 99.7% identity in 311 aa overlap. Contains hydrophobic, FT possible membrane-spanning regions." FT /product="putative membrane protein" FT /tb_orthologue="Rv1456c" FT CDS 713858..714100 FT /class="VI" FT /colour=8 FT /gene="ML0588" FT /ml_unique FT /note="Unknown function. Previously sequenced as TR:Q49686 FT (EMBL:U00013) (80 aa); Fasta score E(): 0, 98.8% identity FT in 80 aa overlap." FT /product="hypothetical protein" FT CDS complement(714234..715031) FT /class="II.C.4" FT /colour=3 FT /gene="ML0589" FT /note="Similar to M. tuberculosis Rv1457c, putative FT membrane protein, TR:O86349 (EMBL:AL123456) (261 aa); FT Fasta score E(): 0, 83.1% identity in 260 aa overlap. FT Similar to bacterial hypothetical membrane proteins e.g. FT Mycobacterium smegmatis fxtE, hypothetical membrane FT protein, TR:O87317 (EMBL:AF027770) (236 aa); Fasta score FT E(): 0, 77.1% identity in 236 aa overlap. Previously FT sequenced as TR:Q49703 (EMBL:U00013) (265 aa); Fasta score FT E(): 0, 99.6% identity in 265 aa overlap. Contains FT hydrophobic, probable membrane-spanning regions." FT /product="putative membrane protein" FT /tb_orthologue="Rv1457c" FT CDS complement(715031..715978) FT /class="III.A" FT /colour=3 FT /gene="ML0590" FT /note="Similar to M. tuberculosis Rv1458c, ABC-transporter FT ATP-binding protein, TR:O53149 (EMBL:AL123456) (313 aa); FT Fasta score E(): 0, 88.0% identity in 308 aa overlap. FT Similar to many e.g. Rhizobium sp. (strain NGR234). nodI, FT nodulation ATP-binding protein I, SW:NODI_RHISN (P55476) FT (343 aa); Fasta score E(): 3.4e-28, 39.4% identity in 317 FT aa overlap. Previously sequenced as TR:Q49707 FT (EMBL:U00013) (315 aa); Fasta score E(): 0, 100.0% FT identity in 315 aa overlap. Contains Pfam match to entry FT PF00005 ABC_tran, ABC transporter. Contains PS00017 FT ATP/GTP-binding site motif A (P-loop). Contains PS00211 FT ABC transporters family signature." FT /product="putative ABC transporter ATP-binding protein" FT /tb_orthologue="Rv1458c" FT RBS complement(715039..715045) FT /note="possible RBS" FT misc_feature complement(715331..715861) FT /colour=0 FT /note="Pfam match to entry PF00005 ABC_tran, ABC FT transporter, score 168.90, E-value 8.6e-47" FT misc_feature complement(715514..715558) FT /colour=8 FT /note="PS00211 ABC transporters family signature" FT misc_feature complement(715817..715840) FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT CDS complement(715975..717756) FT /class="II.C.4" FT /colour=3 FT /gene="ML0591" FT /note="Similar to M. tuberculosis Rv1459c, conserved FT hypothetical protein, TR:O53150 (EMBL:AL123456) (591 aa); FT Fasta score E(): 0, 78.6% identity in 585 aa overlap and FT to Rv2174, putative membrane protein, TR:O53508 FT (EMBL:AL123456) (516 aa); Fasta score E(): 2.2e-26, 31.6% FT identity in 513 aa overlap. Previously sequenced as FT TR:Q49695 (EMBL:U00013) (593 aa); Fasta score E(): 0, FT 100.0% identity in 593 aa overlap. Contains hydrophobic, FT possible membrane-spanning regions." FT /note="Similar to ML0899" FT /product="putative membrane protein" FT /tb_orthologue="Rv1459c" FT CDS 717872..718636 FT /class="I.J.1" FT /colour=9 FT /gene="ML0592" FT /note="Similar to M. tuberculosis Rv1460, hypothetical FT protein, TR:O53151 (EMBL:AL123456) (278 aa); Fasta score FT E(): 0, 79.6% identity in 250 aa overlap. Similar to other FT bacterial hypothetical proteins e.g. Streptomyces FT coelicolor SCC22.08C, putative DNA-binding protein, FT TR:Q9XAD0 (EMBL:AL096839) (252 aa); Fasta score E(): FT 5.6e-30, 45.4% identity in 216 aa overlap. Previously FT sequenced as TR:Q49688 (EMBL:U00013) (254 aa); Fasta score FT E(): 0, 99.6% identity in 254 aa overlap. Contains a FT probable helix-turn-helix motif at aa 215-236 (Score 1054, FT SD +2.78)" FT /product="putative DNA-binding protein" FT RBS 718674..718681 FT /note="possible RBS" FT CDS 718688..721297 FT /class="V" FT /colour=10 FT /gene="ML0593" FT /note="Similar to M. tuberculosis Rv1461, hypothetical FT protein, SW:YE61_MYCTU (O53152) (846 aa); Fasta score E(): FT 0, 54.3% identity in 920 aa overlap. N- and C-termini are FT similar to various bacterial hypothetical proteins e.g. FT Streptomyces coelicolor SCC22.07C, conserved hypothetical FT protein, TR:Q9XAD1 (EMBL:AL096839) (473 aa); Fasta score FT E(): 0, 45.5% identity in 853 aa overlap. Contains an FT intein from amino acids 202-587 inclusive. The intein is FT different to the one in the M. tuberculosis orthologue and FT at a different relative position. Previously sequenced as FT SW:YE61_MYCLE (Q49689) (869 aa); Fasta score E(): 0, 99.9% FT identity in 869 aa overlap. Contains Pfam match to entry FT PF01458 UPF0051, Uncharacterized protein family (UPF0051). FT Contains PS00881 Protein splicing signature." FT /note="C-terminal half is similar to ML0594" FT /product="conserved hypothetical protein" FT misc_feature 720428..720451 FT /colour=8 FT /note="PS00881 Protein splicing signature" FT misc_feature 720458..721219 FT /colour=0 FT /note="Pfam match to entry PF01458 UPF0051, FT Uncharacterized protein family (UPF0051), score 503.40, FT E-value 1.7e-147" FT RBS 721279..721283 FT /note="possible RBS" FT CDS 721294..722472 FT /class="V" FT /colour=10 FT /gene="ML0594" FT /note="Similar to M. tuberculosis Rv1462, conserved FT hypothetical protein, SW:YE62_MYCTU (O53153) (397 aa); FT Fasta score E(): 0, 80.4% identity in 392 aa overlap. FT Similar to many bacterial hypothetical proteins e.g. FT Streptomyces coelicolor SCC22.06C, conserved hypothetical FT protein, TR:Q9XAD2 (EMBL:AL096839) (394 aa); Fasta score FT E(): 0, 58.6% identity in 365 aa overlap. Previously FT sequenced as SW:YE62_MYCLE (Q49682) (392 aa); Fasta score FT E(): 0, 100.0% identity in 392 aa overlap. Contains Pfam FT match to entry PF01458 UPF0051, Uncharacterized protein FT family (UPF0051)." FT /note="Similar to to the C-terminal half of ML0593" FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv1462" FT misc_feature 721588..722376 FT /colour=0 FT /note="Pfam match to entry PF01458 UPF0051, FT Uncharacterized protein family (UPF0051), score 370.50, FT E-value 1.7e-107" FT CDS 722469..723251 FT /class="III.A" FT /colour=3 FT /gene="ML0595" FT /note="Similar to M. tuberculosis Rv1463, putative ABC FT transporter ATP-binding protein, TR:O53154 (EMBL:AL123456) FT (266 aa); Fasta score E(): 0, 85.8% identity in 267 aa FT overlap. Similar to many members of the ABC transporter FT family, including members found in chloroplast genomes FT e.g. Cyanophora paradoxa ycf16, probable ATP-dependent FT transporter, SW:ABCX_CYAPA (P48255) (259 aa); Fasta score FT E(): 0, 54.2% identity in 227 aa overlap. Previously FT sequenced as TR:Q49693 (EMBL:U00013) (260 aa); Fasta score FT E(): 0, 100.0% identity in 260 aa overlap. Contains Pfam FT match to entry PF00005 ABC_tran, ABC transporter. Contains FT PS00017 ATP/GTP-binding site motif A (P-loop)." FT /product="putative ABC transporter ATP-binding protein" FT /tb_orthologue="Rv1463" FT misc_feature 722580..723158 FT /colour=0 FT /note="Pfam match to entry PF00005 ABC_tran, ABC FT transporter, score 89.10, E-value 9e-23" FT misc_feature 722601..722624 FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT RBS 723243..723246 FT /note="possible RBS" FT CDS 723254..724510 FT /class="IV.H" FT /colour=7 FT /gene="ML0596" FT /note="Similar to M. tuberculosis Rv1464, NifS-like FT protein, TR:O53155 (EMBL:AL123456) (417 aa); Fasta score FT E(): 0, 85.4% identity in 417 aa overlap. Similar to many FT putative aminotransferases and to Anabaena sp. (strain PCC FT 7120) nifS, SW:NIFS_ANASP (P12623) (621 aa); BlastP Expect FT 9.9. Previously sequenced as TR:Q49690 (EMBL:U00013) (418 FT aa); Fasta score E(): 0, 100.0% identity in 418 aa FT overlap. Contains Pfam match to entry PF00266 aminotran_5, FT Aminotransferases class-V. Contains PS00595 FT Aminotransferases class-V pyridoxal-phosphate attachment FT site." FT /note="Similar to part of ML0842 and to ML0117 and ML1708" FT /product="putative aminotransferase" FT /tb_orthologue="Rv1464" FT misc_feature 723374..724465 FT /colour=0 FT /note="Pfam match to entry PF00266 aminotran_5, FT Aminotransferases class-V, score 5.00, E-value 1.6e-07" FT misc_feature 723926..723985 FT /colour=8 FT /note="PS00595 Aminotransferases class-V FT pyridoxal-phosphate attachment site" FT RBS 724500..724503 FT /note="possible RBS" FT CDS 724507..725004 FT /class="V" FT /colour=10 FT /gene="ML0597" FT /note="Similar to M. tuberculosis Rv1465, conserved FT hypothetical protein, TR:O53156 (EMBL:AL123456) (162 aa); FT Fasta score E(): 0, 79.4% identity in 165 aa overlap. FT Similar to many hypothetical proteins and shows very weak FT similarity to proteins from N-fixation loci e.g. Anabaena FT azollae nifU, nitrogen fixation protein, SW:NIFU_ANAAZ FT (Q43885) (300 aa); Fasta score E(): 1.9, 24.4% identity in FT 90 aa overlap. Previously sequenced as TR:Q49683 FT (EMBL:U00013) (165 aa); Fasta score E(): 0, 100.0% FT identity in 165 aa overlap. Contains Pfam match to entry FT PF01883 DUF59, Domain of unknown function. Contains Pfam FT match to entry PF01592 NifU_N, NifU-like N terminal FT domain." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv1465" FT misc_feature 724528..724902 FT /colour=0 FT /note="Pfam match to entry PF01592 NifU_N, NifU-like N FT terminal domain, score 152.10, E-value 9.8e-42" FT RBS 724967..724973 FT /note="possible RBS" FT CDS 724979..725326 FT /class="V" FT /colour=10 FT /gene="ML0598" FT /note="Similar to M. tuberculosis Rv1466, conserved FT hypothetical protein, TR:O53157 (EMBL:AL123456) (115 aa); FT Fasta score E(): 0, 82.6% identity in 115 aa overlap. FT Similar to hypothetical proteins located downstream of FT sigma factor genes in both Streptococcus spp. Previously FT sequenced as TR:Q49691 (EMBL:U00013) (115 aa); Fasta score FT E(): 0, 100.0% identity in 115 aa overlap. Contains Pfam FT match to entry PF01883 DUF59, Domain of unknown function." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv1466" FT misc_feature 724985..725296 FT /colour=0 FT /note="Pfam match to entry PF01883 DUF59, Domain of FT unknown function, score 69.30, E-value 7.9e-17" FT CDS complement(725440..727009) FT /class="I.A.3" FT /colour=0 FT /gene="fadE15" FT /gene="ML0599" FT /note="Possible pseudogene of M. tuberculosis orthologue FT fadE15 (Best blastx score 267)" FT /product="acyl-CoA dehydrogenase (pseudogene)" FT /pseudo FT repeat_region 727200..729132 FT CDS complement(731675..732618) FT /class="I.A.3" FT /colour=0 FT /gene="ML0600" FT /note="Possible pseudogene of an acyl-CoA dehydrogenase FT homolgous to those in M. tuberculosis (Best blastx score FT 114)" FT /product="acyl-CoA dehydrogenase (pseudogene)" FT /pseudo FT CDS 733140..733879 FT /class="V" FT /colour=0 FT /gene="ML0601" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2415c (Best blastx score 191)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS 733927..734848 FT /class="V" FT /colour=0 FT /gene="ML0602" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2414c (Best blastx score 167)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS 735002..736117 FT /class="II.C.2" FT /colour=3 FT /gene="ML0603" FT /note="Similar to M. tuberculosis Rv2413c, conserved FT hypothetical protein, TR:P71730 (EMBL:AL123456) (316 aa); FT Fasta score E(): 0, 83.5% identity in 316 aa overlap. Also FT similar to Streptomyces coelicolor SCC123.02C, putative FT DNA-binding protein, TR:Q9RDM2 (EMBL:AL136518) (336 aa); FT Fasta score E(): 0, 39.3% identity in 326 aa overlap. FT Previously sequenced as TR:Q49756 (EMBL:U00016) (389 aa); FT Fasta score E(): 0, 100.0% identity in 371 aa overlap. FT Contains a possible N-terminal signal sequence. Contains FT PS00013 Prokaryotic membrane lipoprotein lipid attachment FT site." FT /product="putative lipoprotein" FT /tb_orthologue="Rv2413c" FT misc_feature 735035..735067 FT /colour=8 FT /note="PS00013 Prokaryotic membrane lipoprotein lipid FT attachment site" FT CDS complement(736154..736414) FT /class="II.A.1" FT /colour=2 FT /gene="rpsT" FT /gene="ML0604" FT /note="Similar to M. tuberculosis rpsT, 30S ribosomal FT protein S20, SW:RS20_MYCTU (P71731) (86 aa); Fasta score FT E(): 8.8e-27, 87.2% identity in 86 aa overlap. Similar to FT many e.g. Escherichia coli rpsT, 30S ribosomal protein FT S20, SW:RS20_ECOLI (P02378) (86 aa); Fasta score E(): FT 1.5e-08, 41.7% identity in 84 aa overlap. Previously FT sequenced as SW:RS20_MYCLE (O33132) (86 aa); Fasta score FT E(): 2.1e-30, 98.8% identity in 86 aa overlap. Contains FT Pfam match to entry PF01649 Ribosomal_S20p, Ribosomal FT protein S20." FT /product="30S ribosomal protein S20" FT /tb_orthologue="rpsT" FT misc_feature complement(736160..736411) FT /colour=0 FT /note="Pfam match to entry PF01649 Ribosomal_S20p, FT Ribosomal protein S20, score 151.30, E-value 1.7e-41" FT RBS complement(736424..736429) FT /note="possible RBS" FT RBS 736541..736544 FT /note="possible RBS" FT CDS 736549..738234 FT /class="V" FT /colour=10 FT /gene="ML0605" FT /note="Similar to M. tuberculosis Rv2411c, conserved FT hypothetical protein, SW:YO11_MYCTU (P71732) (551 aa); FT Fasta score E(): 0, 87.4% identity in 554 aa overlap. FT Similar to other bacterial hypothetical proteins. This CDS FT and the downstream CDS are also similar to the N- and FT C-terminal regions respectively, of longer hypothetical FT proteins from M. tuberculosis and M. leprae e.g. M. FT tuberculosis Rv2567, SW:YP67_MYCTU (Q50654) (884 aa); FT Fasta score E(): 0, 32.2% identity in 537 aa overlap. FT Contains a Gln-rich region at the C-terminus. Previously FT sequnced as SW:YO11_MYCLE (Q49755) (561 aa); Fasta score FT E(): 0, 100.0% identity in 561 aa overlap." FT /note="Similar to the N-terminal region of ML2679" FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv2411c" FT CDS 738234..739211 FT /class="V" FT /colour=10 FT /gene="ML0606" FT /note="Similar to M. tuberculosis Rv2410c, conserved FT hypothetical protein, TR:P71733 (EMBL:AL123456) (325 aa); FT Fasta score E(): 0, 90.8% identity in 325 aa overlap. FT Similar to other bacterial hypothetical proteins. This CDS FT and the upstream CDS are also similar to the C- and FT N-terminal regions respectively, of longer hypothetical FT proteins from M. tuberculosis and M. leprae e.g. M. FT tuberculosis Rv2567, SW:YP67_MYCTU. Previously sequnced as FT TR:Q49770 (EMBL:U00016) (325 aa); Fasta score E(): 0, FT 100.0% identity in 325 aa overlap." FT /note="Similar to the C-terminal region of ML2679" FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv2410c" FT RBS 739200..739203 FT /note="possible RBS" FT CDS 739211..740050 FT /class="V" FT /colour=10 FT /gene="ML0607" FT /note="Similar to M. tuberculosis Rv2409c, conserved FT hypothetical protein, TR:P71734 (EMBL:AL123456) (279 aa); FT Fasta score E(): 0, 82.1% identity in 279 aa overlap and FT to Rv2569c, hypothetical protein, SW:YP69_MYCTU (Q50652) FT (314 aa); Fasta score E(): 4.1e-13, 33.0% identity in 300 FT aa overlap. Similar to other bacterial hypothetical FT proteins e.g. Deinococcus radiodurans DR1993, conserved FT hypothetical protein, TR:Q9RSX6 (EMBL:AE002037) (274 aa); FT Fasta score E(): 8.2e-25, 33.6% identity in 280 aa FT overlap. Previously sequnced as SW:YP69_MYCLE (U00016) FT (279 aa); Fasta score E(): 0, 100.0% identity in 279 aa FT overlap. Contains Pfam match to entry PF01841 FT Transglut_core, Transglutaminase-like superfamily." FT /note="Similar to ML0508" FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv2409c" FT misc_feature 739718..739909 FT /colour=0 FT /note="Pfam match to entry PF01841 Transglut_core, FT Transglutaminase-like superfamily, score 78.40, E-value FT 1.4e-19" FT CDS complement(740723..741531) FT /class="V" FT /colour=0 FT /gene="ML0608" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2407 (Best blastx score 558)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS 741778..741991 FT /class="V" FT /colour=0 FT /gene="ML0609" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2406c (Best blastx score 134)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(742052..742614) FT /class="V" FT /colour=0 FT /gene="ML0610" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2405 (Best blastx score 219)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS 742716..744656 FT /class="I.F.5" FT /colour=7 FT /gene="lepA" FT /gene="ML0611" FT /note="Similar to M. tuberculosis lepA, Rv2404c, putative FT GTP-binding protein, SW:LEPA_MYCTU (P71739) (653 aa); FT Fasta score E(): 0, 88.0% identity in 649 aa overlap. FT Similar to many e.g. Bacillus subtilis lepA, GTP-binding FT protein, SW:LEPA_BACSU (P37949) (612 aa); Fasta score E(): FT 0, 56.8% identity in 613 aa overlap. Previously sequenced FT as SW:LEPA_MYCLE (P53530) (646 aa); Fasta score E(): 0, FT 99.8% identity in 646 aa overlap. Contains Pfam match to FT entry PF00009 GTP_EFTU, Elongation factor Tu family. FT Contains PS00301 GTP-binding elongation factors signature. FT Contains PS00017 ATP/GTP-binding site motif A (P-loop)." FT /note="Show weak imilarity to ML1498, ML1877 and ML1878" FT /product="putative GTP-binding protein" FT /tb_orthologue="lepA" FT misc_feature 742839..743975 FT /colour=0 FT /note="Pfam match to entry PF00009 GTP_EFTU, Elongation FT factor Tu family, score 325.50, E-value 6.1e-94" FT misc_feature 742866..742889 FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT misc_feature 742959..743006 FT /colour=8 FT /note="PS00301 GTP-binding elongation factors signature" FT CDS complement(744738..746654) FT /class="V" FT /colour=0 FT /gene="ML0612" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2402 (Best blastx score 1589)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(746875..747774) FT /class="I.B.7" FT /colour=0 FT /gene="ML0613" FT /note="Possible pseudogene similar to M. tuberculosis FT Rv1751 (Best blastx score 134)" FT /product="possible hydroxylase (pseudogene)" FT /pseudo FT CDS 747777..748064 FT /class="VI" FT /colour=8 FT /gene="ML0614" FT /ml_unique FT /note="Unknown function. Previously sequenced as TR:Q49760 FT (EMBL:U00016) (95 aa); Fasta score E(): 0, 100.0% identity FT in 95 aa overlap." FT /product="hypothetical protein" FT CDS 748382..749428 FT /class="III.A.4" FT /colour=3 FT /gene="subI" FT /gene="ML0615" FT /note="Similar to M. tuberculosis subI, Rv2400c, putative FT sulphate binding protein, TR:P71744 (EMBL:AL123456) (356 FT aa); Fasta score E(): 0, 76.5% identity in 340 aa overlap. FT Similar to many e.g. Escherichia coli sbp, sulfate-binding FT protein precursor, SW:SUBI_ECOLI (P06997) (329 aa); Fasta FT score E(): 1.2e-28, 35.7% identity in 291 aa overlap. FT Previously sequenced as TR:Q49748 (EMBL:U00016) (358 aa); FT Fasta score E(): 0, 100.0% identity in 348 aa overlap. FT Contains Pfam match to entry PF01100 Sulphate_bind, FT Prokaryotic sulphate- and thiosulphate-binding protein." FT /product="putative sulphate-binding protein" FT /tb_orthologue="subI" FT misc_feature 748406..749416 FT /colour=0 FT /note="Pfam match to entry PF01100 Sulphate_bind, FT Prokaryotic sulphate- and thiosulphate-binding protein, FT score 211.20, E-value 1.6e-59" FT CDS 749509..750255 FT /class="III.A.4" FT /colour=0 FT /gene="cysT" FT /gene="ML0616" FT /note="Possible pseudogene of M. tuberculosis orthologue FT cysT (Best blastx score 386)" FT /product="sulphate transport system permease protein FT (pseudogene)" FT /pseudo FT CDS 750258..750925 FT /class="III.A.4" FT /colour=0 FT /gene="cysW" FT /gene="ML0617" FT /note="Possible pseudogene of M. tuberculosis orthologue FT cysW (Best blastx score 281)" FT /product="sulphate transport system permease protein FT (pseudogene)" FT /pseudo FT CDS 751047..751488 FT /class="III.A.4" FT /colour=0 FT /gene="cysA" FT /gene="ML0618" FT /note="Possible pseudogene of M. tuberculosis orthologue FT cysA (Best blastx score 153)" FT /product="sulphate transport ATP-binding protein FT (pseudogene)" FT /pseudo FT CDS complement(751913..752428) FT /class="V" FT /colour=0 FT /gene="ML0619" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2387 (Best blastx score 213)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS 753532..754035 FT /class="II.C.2" FT /colour=3 FT /gene="mtb12" FT /gene="ML0620" FT /note="Similar to M. tuberculosis mtb12, Rv2376c, low FT molecular weight antigen, SW:MB12_MYCTU (O05822) (168 aa); FT Fasta score E(): 0, 65.5% identity in 165 aa overlap. FT Previously sequenced as SW:MB12_MYCLE (Q49771) (165 aa); FT Fasta score E(): 0, 100.0% identity in 165 aa overlap. M. FT tuberculosis mtb12 is secreted, therefore this protein may FT also be secreted." FT /product="putative secreted protein" FT /tb_orthologue="Rv2376c" FT CDS complement(754090..754773) FT /class="II.A.7" FT /colour=0 FT /gene="ML0621" FT /note="Possible pseudogene of gene similar to M. FT tuberculosis sigI gene (Best blastx score 110)" FT /product="ECF family sigma factor (pseudogene)" FT /pseudo FT CDS complement(754965..755390) FT /class="IV.B.1.c" FT /colour=0 FT /gene="ML0622" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3327 (Best blastx score 106)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(755666..755984) FT /class="V" FT /colour=0 FT /gene="ML0623" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2375 (Best blastx score 230)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT RBS 756753..756761 FT /note="possible RBS" FT CDS 756770..757801 FT /class="III.B" FT /colour=0 FT /gene="hrcA" FT /gene="ML0624" FT /note="Similar to M. tuberculosis hrcA, Rv2374c, FT heat-inducible transcription repressor, SW:HRCA_MYCTU FT (O05824) (343 aa); Fasta score E(): 0, 89.5% identity in FT 343 aa overlap. Similar to many e.g. Bacillus FT stearothermophilus, hcrA, heat-inducible transcription FT repressor, SW:HRCA_BACST (Q45550) (344 aa); Fasta score FT E(): 3.5e-26, 28.3% identity in 336 aa overlap. Previously FT sequenced as TR:Q49749 (EMBL:U00016) (197 aa); Fasta score FT E(): 0, 99.5% identity in 196 aa overlap. Contains Pfam FT match to entry PF01628 HrcA, HrcA protein." FT /product="putative heat-inducible transcription repressor" FT /tb_orthologue="hrcA" FT misc_feature 756776..757768 FT /colour=0 FT /note="Pfam match to entry PF01628 HrcA, HrcA protein, FT score 472.40, E-value 3.6e-138" FT RBS 757860..757865 FT /note="possible RBS" FT CDS 757873..759009 FT /class="III.B" FT /colour=0 FT /gene="dnaJ2" FT /gene="ML0625" FT /note="Similar to M. tuberculosis dnaJ2, Rv2373c, DnaJ2 FT protein, TR:O05825 (EMBL:AL123456) (382 aa); Fasta score FT E(): 0, 88.0% identity in 382 aa overlap. Similar to many FT e.g. Escherichia coli dnaJ, Dna protein, SW:DNAJ_ECOLI FT (P08622) (375 aa); Fasta score E(): 0, 40.6% identity in FT 382 aa overlap. Previously sequenced as TR:Q49762 FT (EMBL:U00016) (378 aa); Fasta score E(): 0, 99.7% identity FT in 378 aa overlap. Contains Pfam match to entry PF01556 FT DnaJ_C, DnaJ C terminal region. Contains Pfam match to FT entry PF00684 DnaJ_CXXCXGXG, DnaJ central domain (4 FT repeats). Contains Pfam match to entry PF00226 DnaJ, DnaJ FT domain." FT /note="Similar to ML2494" FT /product="DnaJ homologue" FT /tb_orthologue="dnaJ2" FT misc_feature 757882..758076 FT /colour=0 FT /note="Pfam match to entry PF00226 DnaJ, DnaJ domain, FT score 131.40, E-value 1.6e-35" FT misc_feature 758254..758508 FT /colour=0 FT /note="Pfam match to entry PF00684 DnaJ_CXXCXGXG, DnaJ FT central domain (4 repeats), score 70.80, E-value 2.9e-17" FT misc_feature 758545..758913 FT /colour=0 FT /note="Pfam match to entry PF01556 DnaJ_C, DnaJ C terminal FT region, score 229.20, E-value 6.1e-65" FT CDS 759041..759814 FT /class="V" FT /colour=10 FT /gene="ML0626" FT /note="Similar to M. tuberculosis Rv2372c, conserved FT hypothetical protein, TR:O05826 (EMBL:AL123456) (262 aa); FT Fasta score E(): 0, 76.9% identity in 255 aa overlap. FT Similar to many bascterial hypothetical proteins e.g. FT Streptomyces coelicolor sdrD, hypothetical protein, FT TR:Q9S3Y4 (EMBL:AF170561) (216 aa); Fasta score E(): FT 1.2e-28, 44.8% identity in 223 aa overlap. Previously FT sequenced as TR:Q49763 (EMBL:U00016) (128 aa); Fasta score FT E(): 0, 100.0% identity in 126 aa overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv2372c" FT CDS 760114..761163 FT /class="I.A.4" FT /colour=7 FT /gene="phoH" FT /gene="ML0627" FT /note="Similar to M. tuberculosis phoH, Rv2372c, FT phoH-family protein, SW:PHOL_MYCTU (O05830) (352 aa); FT Fasta score E(): 0, 88.9% identity in 352 aa overlap. FT Similar to many hypothetical proteins and to Escherichia FT coli phoH, phosphate starvation-inducible protein, FT SW:PHOH_ECOLI (P31544) (354 aa); Fasta score E(): 1.2e-29, FT 36.8% identity in 304 aa overlap. Previously sequenced as FT SW:PHOL_MYCLE (Q49751) (349 aa); Fasta score E(): 0, 99.7% FT identity in 349 aa overlap. Contains PS00017 FT ATP/GTP-binding site motif A (P-loop)." FT /note="Similar to ML1951, except and N- and C-termini" FT /product="phoH-family protein" FT /tb_orthologue="phoH" FT misc_feature 760552..760575 FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT CDS 761194..761730 FT /class="V" FT /colour=10 FT /gene="ML0628" FT /note="Similar to M. tuberculosis Rv2367c, hypothetical FT protein, SW:YN67_MYCTU (O05831) (182 aa); Fasta score E(): FT 0, 89.1% identity in 175 aa overlap. Similar to many FT bacterial hypothetical proteins e.g. Streptomyces FT coelicolor SCC117.06, hypothetical protein, TR:CAB66427 FT (EMBL:AL136534) (165 aa); Fasta score E(): 1e-31, 54.5% FT identity in 154 aa overlap. Similar to Streptococcus FT mutans dgk, diacyglycerol kinase, TR:O51806 FT (EMBL:AF000954) (164 aa); Fasta score E(): 3.8e-07, 30.9% FT identity in 162 aa overlap. Previously sequenced as FT SW:YN67_MYCLE (Q49752) (178 aa); Fasta score E(): 0, FT 100.0% identity in 178 aa overlap. Contains Pfam match to FT entry PF02130 UPF0054, Uncharacterized protein family FT UPF0054. Contains PS01306 Uncharacterized protein family FT UPF0054 signature." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv2367c" FT misc_feature 761311..761631 FT /colour=0 FT /note="Pfam match to entry PF02130 UPF0054, FT Uncharacterized protein family UPF0054, score 235.30, FT E-value 8.7e-67" FT misc_feature 761545..761577 FT /colour=8 FT /note="PS01306 Uncharacterized protein family UPF0054 FT signature" FT CDS 761727..763012 FT /class="V" FT /colour=0 FT /gene="ML0629" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2366c (Best blastx score 651)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT RBS 763019..763023 FT /note="possible RBS" FT CDS 763032..763358 FT /class="V" FT /colour=10 FT /gene="ML0630" FT /note="Similar to M. tuberculosis Rv2365c, hypothetical FT protein, TR:O05833 (EMBL:AL123456) (113 aa); Fasta score FT E(): 1.5e-22, 67.9% identity in 106 aa overlap. Similar to FT Streptomyces coelicolor SCC77.05, hypothetical protein, FT TR:Q9RDF3 (EMBL:AL136503) (132 aa); Fasta score E(): FT 3.3e-06, 39.4% identity in 99 aa overlap. Previously FT sequenced as TR:Q49767 (EMBL:U00016) (108 aa); Fasta score FT E(): 0, 100.0% identity in 108 aa overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv2365c" FT CDS 763476..764384 FT /class="I.F.5" FT /colour=7 FT /gene="era" FT /gene="ML0631" FT /note="Similar to M. tuberculosis era, Rv2364c, FT GTP-binding protein Era homolog, SW:ERA_MYCTU (O05834) FT (300 aa); Fasta score E(): 0, 81.4% identity in 301 aa FT overlap. Similar to many bacterial era-homologues and to FT Escherichia coli era, GTP-binding protein, SW:ERA_ECOLI FT (P06616) (301 aa); Fasta score E(): 3.3e-30, 34.3% FT identity in 297 aa overlap. Previously sequenced as FT SW:ERA_MYCLE (Q49768) (302 aa); Fasta score E(): 0, 99.7% FT identity in 302 aa overlap. Contains PS00017 FT ATP/GTP-binding site motif A (P-loop)." FT /product="putative Era-family GTP-binding protein" FT /tb_orthologue="era" FT misc_feature 763518..763541 FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT CDS complement(764433..765812) FT /class="IV.I" FT /colour=0 FT /gene="amiA2" FT /gene="ML0632" FT /note="Possible pseudogene of M. tuberculosis orthologue FT amiA2 (Best blastx score 975)" FT /product="putative amidase (pseudogene)" FT /pseudo FT CDS 765911..766717 FT /class="V" FT /colour=10 FT /gene="ML0633" FT /note="Similar to M. tuberculosis Rv2362c, conserved FT hypothetical protein, TR:O05836 (EMBL:AL123456) (265 aa); FT Fasta score E(): 0, 86.6% identity in 268 aa overlap. FT Similar to Streptomyces coelicolor SCC121.13C, FT hypothetical protein, TR:CAB69731 (EMBL:AL137166) (251 FT aa); Fasta score E(): 0, 52.8% identity in 248 aa overlap. FT Shows weak similarity, except at C-terminus to Bacillus FT subtilis recO, DNA repair protein, SW:RECO_BACSU (P42095) FT (255 aa); Fasta score E(): 5.3e-11, 27.3% identity in 176 FT aa overlap. Previously sequenced as TR:Q49754 FT (EMBL:U00016) (269 aa); Fasta score E(): 0, 100.0% FT identity in 268 aa overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv2362c" FT CDS 766710..767600 FT /EC_number="2.5.1.31" FT /class="II.C.3" FT /colour=3 FT /gene="ML0634" FT /note="Similar to M. tuberculosis Rv2361c, putative FT undecaprenyl pyrophosphate synthetase, SW:UPPS_MYCTU FT (O05837) (296 aa); Fasta score E(): 0, 86.5% identity in FT 296 aa overlap. Similar to many e.g. Micrococcus luteus FT uppS, undecaprenyl pyrophosphate synthetase, SW:UPPS_MICLU FT (O82827) (249 aa); Fasta score E(): 5.4e-30, 39.4% FT identity in 246 aa overlap. Previously sequenced as FT SW:UPPS_MYCLE (P38119) (296 aa); Fasta score E(): 0, 99.7% FT identity in 296 aa overlap. Contains Pfam match to entry FT PF01255 UPP_synthetase, Putative undecaprenyl diphosphate FT synthase. Contains PS01066 Undecaprenyl pyrophosphate FT synthetase family signature." FT /note="Similar to ML2467" FT /product="putative undecaprenyl pyrophosphate synthetase" FT /tb_orthologue="Rv2361c" FT misc_feature 766929..767594 FT /colour=0 FT /note="Pfam match to entry PF01255 UPP_synthetase, FT Putative undecaprenyl diphosphate synthase, score 487.70, FT E-value 8.8e-143" FT misc_feature 767427..767480 FT /colour=8 FT /note="PS01066 Undecaprenyl pyrophosphate synthetase FT family signature" FT CDS 767600..767932 FT /class="V" FT /colour=0 FT /gene="ML0635" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2360c (Best blastx score 192)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(767995..768473) FT /class="II.C.1" FT /colour=0 FT /gene="ML0636" FT /note="Possible pseudogene similar to M. tuberculosis lpqQ FT (Best blastx score 118)" FT /product="lipoprotein (pseudogene)" FT /pseudo FT CDS 769118..771271 FT /class="II.A.5" FT /colour=2 FT /gene="uvrD2" FT /gene="ML0637" FT /note="Similar to M. tuberculosis uvrD2, Rv3198c, putative FT ATP-dependent DNA helicase, SW:UVRD_MYCTU (O53344) (700 FT aa); Fasta score E(): 0, 82.9% identity in 706 aa overlap. FT Similar to many e.g. Escherichia coli uvrD, DNA helicase FT II, SW:UVRD_ECOLI (P03018) (720 aa); Fasta score E(): FT 1.1e-20, 32.6% identity in 660 aa overlap. Previously FT sequenced as SW:UVRD_MYCLE (P53528) (457 aa); Fasta score FT E(): 0, 100.0% identity in 439 aa overlap. Contains Pfam FT match to entry PF00570 HRDC, HRDC domain. Contains Pfam FT match to entry PF00580 UvrD-helicase, UvrD/REP helicase. FT Contains PS00017 ATP/GTP-binding site motif A (P-loop)." FT /note="ML0153" FT /product="putative ATP-dependent DNA helicase" FT /tb_orthologue="uvrD2" FT misc_feature 769163..770626 FT /colour=0 FT /note="Pfam match to entry PF00580 UvrD-helicase, UvrD/REP FT helicase, score 191.00, E-value 1.9e-53" FT misc_feature 769217..769240 FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT misc_feature 771023..771268 FT /colour=0 FT /note="Pfam match to entry PF00570 HRDC, HRDC domain, FT score 54.00, E-value 3.4e-12" FT RBS 771412..771416 FT /note="possible RBS" FT CDS 771426..771728 FT /class="II.C.2" FT /colour=3 FT /gene="ML0638" FT /note="Unknown function. Previously sequenced as TR:Q49769 FT (EMBL:U00016) (100 aa); Fasta score E(): 0, 99.0% identity FT in 100 aa overlap. Contains a possible N-terminal signal FT sequence." FT /product="putative secreted protein" FT RBS 771715..771720 FT /note="possible RBS" FT CDS 771722..771991 FT /class="I.J.1" FT /colour=9 FT /gene="whmC" FT /gene="ML0639" FT /note="Similar to M. tuberculosis Jv1021c, probable FT DNA-binding protein, TR:Q9S4Q3 (EMBL:AF121014) (134 aa); FT Fasta score E(): 9.6e-25, 69.3% identity in 88 aa overlap FT (from mRNA). Shows weak similarity to members of the FT whiB-family of transcriptional regulators e.g. FT Streptomyces coelicolor whiD, WhiD protein, TR:O88103 FT (EMBL:AJ010601) (112 aa); Fasta score E(): 0.0045, 34.7% FT identity in 72 aa overlap. Previously sequenced as FT TR:Q49765 (EMBL:U00016) (89 aa); Fasta score E(): 0, FT 100.0% identity in 89 aa overlap." FT /note="Similar to ML0382, ML0760, ML0804 and ML2307" FT /product="putative transcriptional regulator" FT CDS complement(772068..773489) FT /class="V" FT /colour=10 FT /gene="ML0640" FT /note="Similar to M. tuberculosis Rv3197, putative ABC FT transporter, TR:O53343 (EMBL:AL123456) (447 aa); Fasta FT score E(): 0, 83.0% identity in 447 aa overlap. Similar to FT many ABC1-like hypothetical proteins from bacteria and FT eukaryotes. Similar to Saccharomyces cerevisia ABC1, ABC1 FT protein precursor, SW:ABC1_YEAST (P27697) (501 aa); Fasta FT score E(): 7.3e-19, 24.9% identity in 382 aa overlap. FT Previously sequenced as TR:Q49747 (EMBL:U00016) (267 aa); FT Fasta score E(): 0, 99.6% identity in 248 aa overlap. FT Contains PS00017 ATP/GTP-binding site motif A (P-loop)." FT /note="Similar to ML1898" FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv3197" FT misc_feature complement(773274..773297) FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT CDS complement(773607..774443) FT /class="V" FT /colour=0 FT /gene="ML0641" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3196 (Best blastx score 289)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(774506..775945) FT /class="V" FT /colour=10 FT /gene="ML0642" FT /note="Similar to M. tuberculosis Rv3195, conserved FT hypothetical protein, TR:O53341 (EMBL:AL123456) (472 aa); FT Fasta score E(): 0, 79.2% identity in 475 aa overlap. FT Similar to Streptomyces coelicolor SCE9.14C, hypothetical FT protein, TR:Q9X8I7 (EMBL:AL049841) (375 aa); Fasta score FT E(): 4.9e-12, 24.9% identity in 305 aa overlap. previously FT sequenced as TR:Q49746 (EMBL:U00016) (479 aa); Fasta score FT E(): 0, 99.8% identity in 479 aa overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv3195" FT CDS 776008..777030 FT /class="II.C.2" FT /colour=3 FT /gene="ML0643" FT /note="Similar to M. tuberculosis Rv3194c, conserved FT hypothetical protein, TR:O53340 (EMBL:AL123456) (340 aa); FT Fasta score E(): 0, 80.3% identity in 340 aa overlap. FT Similar to bacterial hypothetical proteins e.g. FT Streptomyces coelicolor sdrC, hypothetical protein, FT TR:Q9S3Y5 (EMBL:AF170560) (241 aa); Fasta score E(): FT 5.5e-18, 34.9% identity in 318 aa overlap. Contains a FT possible N-terminal signal sequence." FT /product="putative secreted protein" FT /tb_orthologue="Rv3194c" FT RBS 777164..777167 FT /note="possible RBS" FT CDS 777176..780127 FT /class="II.C.4" FT /colour=3 FT /gene="ML0644" FT /note="Similar to M. tuberculosis Rv3193c, putative FT integral membrane protein, TR:O53339 (EMBL:AL123456) (992 FT aa); Fasta score E(): 0, 86.1% identity in 989 aa overlap FT and to Rv0064, putative membrane protein, TR:O53609 FT (EMBL:AL123456) (979 aa); Fasta score E(): 0, 49.3% FT identity in 984 aa overlap. Similar to bacterial FT hypothetical membrane proteins. Previously sequenced as FT TR:O32904 (EMBL:Z98271) (277 aa); Fasta score E(): 0, FT 100.0% identity in 277 aa overlap. Contains hydrophobic, FT possible membrane-spanning regions." FT /product="putative integral membrane protein" FT /tb_orthologue="Rv3193c" FT tRNA 780284..780360 FT /colour=4 FT /note="tRNA Met anticodon CAT, Cove score 88.77" FT CDS 782071..782519 FT /class="V" FT /colour=0 FT /gene="ML0645" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3172c (Best blastx score 176)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS 783177..783857 FT /class="III.F" FT /colour=0 FT /gene="hpx" FT /gene="ML0646" FT /note="Possible pseudogene of M. tuberculosis orthologue FT hpx (Best blastx score 187)" FT /product="probable non-heme haloperoxidase (pseudogene)" FT /pseudo FT CDS complement(783902..785173) FT /class="I.B.7" FT /colour=0 FT /gene="ML0647" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3170 (Best blastx score 441)" FT /product="Probable flavin-containing monoamine oxidase FT (pseudogene)" FT /pseudo FT CDS complement(786098..787165) FT /class="V" FT /colour=0 FT /gene="ML0648" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3169 (Best blastx score 168)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(787162..788296) FT /class="V" FT /colour=0 FT /gene="ML0649" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3168 (Best blastx score 320)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS 788362..788662 FT /class="I.J.1" FT /colour=0 FT /gene="ML0650" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3167c (Best blastx score 210)" FT /product="putative transcriptional regulator (pseudogene)" FT /pseudo FT CDS complement(788694..789822) FT /class="IV.B.2" FT /colour=0 FT /gene="ML0651" FT /note="Possible pseudogene similar to M. tuberculosis FT REP-family proteins (Best blastx score 93)" FT /product="REP-family protein (pseudogene)" FT /pseudo FT CDS 790108..791036 FT /class="V" FT /colour=0 FT /gene="ML0652" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3166c (Best blastx score 276)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS 791048..791528 FT /class="V" FT /colour=0 FT /gene="ML0653" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3165c (Best blastx score 151)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS 791657..792392 FT /class="I.J.1" FT /colour=0 FT /gene="moxR3" FT /gene="ML0654" FT /note="Possible pseudogene of M. tuberculosis orthologue FT moxR3 (Best blastx score 249)" FT /product="transcriptional regulator, MoxR homologue FT (pseudogene)" FT /pseudo FT CDS 792430..793542 FT /class="II.C.4" FT /colour=0 FT /gene="ML0655" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3163c (Best blastx score 376)" FT /product="possible membrane protein (pseudogene)" FT /pseudo FT CDS complement(793592..793873) FT /class="VI" FT /colour=8 FT /gene="ML0656" FT /ml_unique FT /note="Unknown function. Previously sequenced as TR:O32893 FT (EMBL:Z98271) (93 aa); Fasta score E(): 0, 100.0% identity FT in 93 aa overlap." FT /product="hypothetical protein" FT CDS complement(794101..794681) FT /class="I.B.6.a" FT /colour=0 FT /gene="nuoN" FT /gene="ML0657" FT /note="Possible pseudogene of M. tuberculosis orthologue FT nuoN (Best blastx score 137)" FT /product="NADH dehydrogenase chain N (pseudogene)" FT /pseudo FT CDS complement(794994..795483) FT /class="I.A.3" FT /colour=0 FT /gene="fadB4" FT /gene="ML0658" FT /note="Possible pseudogene of M. tuberculosis orthologue FT fadB4 (Best blastx score 267)" FT /product="3-hydroxyacyl-CoA dehydrogenase (pseudogene)" FT /pseudo FT CDS complement(795537..795863) FT /class="VI" FT /colour=8 FT /gene="ML0659" FT /ml_unique FT /note="Unknown function. Previously sequenced as TR:O32892 FT (EMBL:Z98271) (108 aa); Fasta score E(): 0, 100.0% FT identity in 108 aa overlap." FT /product="hypothetical protein" FT RBS complement(795874..795877) FT /note="possible RBS" FT CDS complement(796047..797249) FT /class="I.A.3" FT /colour=1 FT /gene="fadE23" FT /gene="ML0660" FT /note="Similar to M. tuberculosis fadE23, Rv3140, acyl-CoA FT dehydrogenase, TR:P95186 (EMBL:AL123456) (401 aa); Fasta FT score E(): 0, 89.5% identity in 401 aa overlap. Similar to FT many e.g. Homo sapiens acadS, acyl-coa dehydrogenase, FT short-chain specific precursor, SW:ACDS_HUMAN (P16219) FT (412 aa); Fasta score E(): 4.1e-31, 31.8% identity in 393 FT aa overlap. Previously sequenced as TR:O32891 FT (EMBL:Z98271) (400 aa); Fasta score E(): 0, 100.0% FT identity in 400 aa overlap. Contains 2 Pfam matches to FT entry PF00441 Acyl-CoA_dh, Acyl-CoA dehydrogenase." FT /note="Similar to ML0737" FT /product="putative acyl-CoA dehydrogenase" FT /tb_orthologue="fadE23" FT misc_feature complement(796083..796349) FT /colour=0 FT /note="Pfam match to entry PF00441 Acyl-CoA_dh, Acyl-CoA FT dehydrogenase, score 65.60, E-value 2e-18" FT misc_feature complement(796437..796868) FT /colour=0 FT /note="Pfam match to entry PF00441 Acyl-CoA_dh, Acyl-CoA FT dehydrogenase, score 162.30, E-value 8.6e-47" FT CDS complement(797292..798689) FT /class="I.A.3" FT /colour=1 FT /gene="fadE24" FT /gene="ML0661" FT /note="Similar to M. tuberculosis fadE24, Rv3139, acyl-CoA FT dehydrogenase, TR:P95187 (EMBL:AL123456) (468 aa); Fasta FT score E(): 0, 83.6% identity in 464 aa overlap. Similar to FT many e.g. Streptomyces lividans fadE, fatty acid acyl-CoA FT dehydrogenase, TR:Q9RIQ5 (EMBL:AJ250495) (385 aa); Fasta FT score E(): 5.1e-27, 28.5% identity in 376 aa overlap. FT Previously sequenced as TR:O32890 (EMBL:Z98271) (465 aa); FT Fasta score E(): 0, 100.0% identity in 465 aa overlap. FT Contains 2 Pfam matches to entry PF00441 Acyl-CoA_dh, FT Acyl-CoA dehydrogenase. Contains PS00073 Acyl-CoA FT dehydrogenases signature 2." FT /note="Similar to ML0737" FT /product="putative acyl-CoA dehydrogenase" FT /tb_orthologue="fadE24" FT misc_feature complement(797328..797672) FT /colour=0 FT /note="Pfam match to entry PF00441 Acyl-CoA_dh, Acyl-CoA FT dehydrogenase, score 116.20, E-value 2.9e-33" FT misc_feature complement(797334..797393) FT /colour=8 FT /note="PS00073 Acyl-CoA dehydrogenases signature 2" FT misc_feature complement(798216..798368) FT /colour=0 FT /note="Pfam match to entry PF00441 Acyl-CoA_dh, Acyl-CoA FT dehydrogenase, score 15.70, E-value 0.00088" FT CDS complement(798766..799533) FT /class="IV.I" FT /colour=7 FT /gene="ML0662" FT /note="Similar to M. tuberculosis Rv3137, putative FT monophosphatase, TR:P95189 (EMBL:AL123456) (260 aa); Fasta FT score E(): 0, 81.8% identity in 253 aa overlap. Similar to FT putative N-amidino-scyllo-inosamine-4-phosphate FT phosphatases from Streptomyces spp. e.g. Streptomyces FT glaucescens strO, TR:P72460 (EMBL:Y08764) (256 aa); Fasta FT score E(): 9.8e-19, 36.2% identity in 229 aa overlap. FT Previously sequenced as TR:O32889 (EMBL:Z98271) (255 aa); FT Fasta score E(): 0, 100.0% identity in 255 aa overlap. FT Contains 2 Pfam matches to entry PF00459 inositol_P, FT Inositol monophosphatase family. Contains PS00629 Inositol FT monophosphatase family signature 1." FT /note="Similar to ML1024" FT /product="putative monophosphatase" FT /tb_orthologue="Rv3137" FT misc_feature complement(798865..798984) FT /colour=0 FT /note="Pfam match to entry PF00459 inositol_P, Inositol FT monophosphatase family, score 24.20, E-value 8.5e-06" FT misc_feature complement(799150..799362) FT /colour=0 FT /note="Pfam match to entry PF00459 inositol_P, Inositol FT monophosphatase family, score 69.30, E-value 1.6e-18" FT misc_feature complement(799273..799314) FT /colour=8 FT /note="PS00629 Inositol monophosphatase family signature FT 1" FT CDS 799661..799975 FT /class="VI" FT /colour=8 FT /gene="ML0663" FT /ml_unique FT /note="Unknown function. Previously sequenced as TR:O32888 FT (EMBL:Z98271) (104 aa); Fasta score E(): 0, 99.0% identity FT in 104 aa overlap." FT /product="hypothetical protein" FT CDS 800116..800379 FT /class="VI" FT /colour=8 FT /gene="ML0664" FT /ml_unique FT /note="Unknown function." FT /product="hypothetical protein" FT CDS 800705..801966 FT /class="I.B.7" FT /colour=0 FT /gene="ML0665" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3107c (Best blastx score 234)" FT /product="Some similarity to D-lactate dehydrogenase FT (pseudogene)" FT /pseudo FT CDS complement(801976..803346) FT /class="I.B.6.c" FT /colour=7 FT /gene="fprA" FT /gene="ML0666" FT /note="Similar to M. tuberculosis fprA, Rv3106, putative FT ferredoxin reductase, TR:O05783 (EMBL:AL123456) (456 aa); FT Fasta score E(): 0, 80.8% identity in 459 aa overlap. FT Similar to adrenodoxin reductases e.g. Bos taurus fdxR, FT NADPH:adrenodoxin oxidoreductase precursor, SW:ADRO_BOVIN FT (P08165) (492 aa); Fasta score E(): 0, 40.9% identity in FT 465 aa overlap. Previously sequenced as TR:O32886 FT (EMBL:Z98271) (456 aa); Fasta score E(): 0, 99.8% identity FT in 456 aa overlap." FT /note="Similar to ML2134" FT /product="putative NADPH-ferredoxin reductase" FT /tb_orthologue="fprA" FT CDS 803526..804650 FT /class="II.A.6" FT /colour=2 FT /gene="prfB" FT /gene="ML0667" FT /note="Similar to M. tuberculosis prfB, Rv3105c, peptide FT chain release factor 2, SW:RF2_MYCTU (O05782) (371 aa); FT Fasta score E(): 0, 90.1% identity in 372 aa overlap. FT Similar to many e.g. Streptomyces coelicolor prfB, peptide FT chain release factor 2, SW:RF2_STRCO (Q53915) (368 aa); FT Fasta score E(): 0, 66.9% identity in 369 aa overlap. FT Previously sequenced as SW:RF2_MYCLE (O32885) (374 aa); FT Fasta score E(): 0, 99.7% identity in 374 aa overlap. FT Contains Pfam match to entry PF00472 RF-1, Peptidyl-tRNA FT hydrolase domain. Contains PS00745 Prokaryotic-type class FT I peptide chain release factors signature." FT /note="Similar to ML1134" FT /product="putative peptide chain release factor 2" FT /tb_orthologue="prfB" FT misc_feature 804210..804548 FT /colour=0 FT /note="Pfam match to entry PF00472 RF-1, Peptidyl-tRNA FT hydrolase domain, score 216.20, E-value 4.8e-61" FT misc_feature 804270..804320 FT /colour=8 FT /note="PS00745 Prokaryotic-type class I peptide chain FT release factors signature" FT CDS 804680..805479 FT /class="V" FT /colour=0 FT /gene="ML0668" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3104c (Best blastx score 303)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS 805920..806609 FT /class="III.C" FT /colour=3 FT /gene="ftsE" FT /gene="ML0669" FT /note="Similar to M. tuberculosis ftsE, Rv3102c, cell FT division ATP-binding protein, TR:O05779 (EMBL:AL123456) FT (229 aa); Fasta score E(): 0, 91.7% identity in 229 aa FT overlap. Similar to many e.g. Escherichia coli ftsE, cell FT division ATP-binding protein, SW:FTSE_ECOLI (P10115) (222 FT aa); Fasta score E(): 0, 49.1% identity in 216 aa overlap. FT Previously sequenced as TR:O32883 (EMBL:Z98271) (229 aa); FT Fasta score E(): 0, 100.0% identity in 229 aa overlap. FT Contains Pfam match to entry PF00005 ABC_tran, ABC FT transporter. Contains PS00017 ATP/GTP-binding site motif A FT (P-loop). Contains PS00211 ABC transporters family FT signature." FT /product="putative cell division ATP-binding protein" FT /tb_orthologue="ftsE" FT misc_feature 806004..806561 FT /colour=0 FT /note="Pfam match to entry PF00005 ABC_tran, ABC FT transporter, score 216.00, E-value 5.6e-61" FT misc_feature 806025..806048 FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT misc_feature 806334..806378 FT /colour=8 FT /note="PS00211 ABC transporters family signature" FT CDS 806610..807503 FT /class="II.C.4" FT /colour=3 FT /gene="ftsX" FT /gene="ML0670" FT /note="Similar to M. tuberculosis ftsX, Rv3101c, cell FT division protein ftsX homolog, SW:FTSX_MYCTU (P96293) (297 FT aa); Fasta score E(): 0, 80.8% identity in 297 aa overlap. FT Similar to many ftsX homologues e.g. Bacillus subtilis FT ftsX, cell division protein ftsX homolog, SW:FTSX_BACSU FT (O34876) (296 aa); Fasta score E(): 2.5e-14, 25.8% FT identity in 302 aa overlap. Previously sequenced as FT SW:FTSX_MYCLE (O32882) (287 aa); Fasta score E(): 0, 99.7% FT identity in 287 aa overlap. Contains hydrophobic, possible FT membrane-spanning regions." FT /product="putative membrane protein" FT /tb_orthologue="ftsX" FT CDS 807506..807988 FT /class="II.B.3" FT /colour=7 FT /gene="smpB" FT /gene="ML0671" FT /note="Similar to M. tuberculosis smpB, Rv3100c, FT SsrA-binding protein, SW:SMPB_MYCTU (P96294) (160 aa); FT Fasta score E(): 0, 84.9% identity in 159 aa overlap. FT Similar to many e.g. Escherichia coli smpB, SSRA-binding FT protein essential for the peptide-tagging activity of SsrA FT (tmRNA), SW:SMPB_ECOLI (P32052) (159 aa); Fasta score E(): FT 4.1e-19, 41.8% identity in 146 aa overlap. Previously FT sequenced as SW:SMPB_MYCLE (O32881) (160 aa); Fasta score FT E(): 0, 99.4% identity in 160 aa overlap. Contains Pfam FT match to entry PF01668 SmpB, SmpB protein. Contains FT PS01317 Protein smpB signature." FT /product="putative SsrA-binding protein" FT /tb_orthologue="smpB" FT misc_feature 807539..807736 FT /colour=0 FT /note="Pfam match to entry PF01668 SmpB, SmpB protein, FT score 145.70, E-value 8.1e-40" FT misc_feature 807593..807631 FT /colour=8 FT /note="PS01317 Protein smpB signature" FT CDS 808148..808841 FT /class="V" FT /colour=0 FT /gene="ML0672" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3099c (Best blastx score 271)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT misc_RNA 809055..809422 FT /colour=4 FT /note="tmRNA (10Sa RNA or SsrA) gene (see FT http://www.indiana.edu/~tmrna/)" FT /label=tmRNA FT CDS complement(809936..810483) FT /class="I.B.7" FT /colour=0 FT /gene="ML0673" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3359 (Best blastx score 307)" FT /product="probable oxidoreductase (pseudogene)" FT /pseudo FT CDS 810692..811540 FT /EC_number="1.5.1.5" FT /EC_number="3.5.4.9" FT /class="I.G.2" FT /colour=7 FT /gene="folD" FT /gene="ML0674" FT /note="Similar to M. tuberculosis folD, Rv3356c, putative FT methylenetetrahydrofolate dehydrogenase, TR:O50385 FT (EMBL:AL123456) (281 aa); Fasta score E(): 0, 86.5% FT identity in 281 aa overlap. Similar to many e.g. FT Escherichia coli folD, bifunctional FT methylenetetrahydrofolate dehydrogenase / FT methenyltetrahydrofolate cyclohydrolase, SW:FOLD_ECOLI FT (P24186) (287 aa); Fasta score E(): 0, 40.8% identity in FT 277 aa overlap. Previously sequenced as TR:O32879 FT (EMBL:Z98271) (282 aa); Fasta score E(): 0, 99.6% identity FT in 282 aa overlap. Contains Pfam match to entry PF00763 FT THF_DHG_CYH, Tetrahydrofolate FT dehydrogenase/cyclohydrolase." FT /product="putative methylenetetrahydrofolate FT dehydrogenase/methenyltetrahydrofolate cyclohydrolase" FT /tb_orthologue="folD" FT misc_feature 810734..811525 FT /colour=0 FT /note="Pfam match to entry PF00763 THF_DHG_CYH, FT Tetrahydrofolate dehydrogenase/cyclohydrolase, score FT 547.30, E-value 1.1e-160" FT CDS 811543..811818 FT /class="V" FT /colour=10 FT /gene="ML0675" FT /note="Similar to M. tuberculosis Rv3346c, conserved FT hypothetical protein, TR:O50384 (EMBL:AL123456) (97 aa); FT Fasta score E(): 1.2e-24, 77.9% identity in 95 aa overlap FT (EMBL:AL123456) (97 aa); Fasta score E(): 1.2e-24, 77.9% FT identity in 95 aa overlap and Rv3355c, conserved FT hypothetical protein, TR:O50384. Previously sequenced as FT TR:O32878 (EMBL:Z98271) (91 aa); Fasta score E(): 0, 98.9% FT identity in 91 aa overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv3346c" FT CDS complement(811815..812291) FT /class="V" FT /colour=10 FT /gene="ML0676" FT /note="Similar to M. tuberculosis Rv3354, conserved FT hypothetical protein, TR:O50383 (EMBL:AL123456) (129 aa); FT Fasta score E(): 6.2e-24, 55.9% identity in 127 aa overlap FT and Rv1690, hypothetical protein, TR:O33192 FT (EMBL:AL123456) (127 aa); Fasta score E(): 2e-09, 33.3% FT identity in 105 aa overlap. Previously sequenced as FT TR:O32877 (EMBL:Z98271) (168 aa); Fasta score E(): 0, FT 100.0% identity in 158 aa overlap." FT /product="hypothetical protein" FT CDS 812527..813381 FT /class="I.B.7" FT /colour=0 FT /gene="ML0677" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv0063 (Best blastx score 243)" FT /product="probable oxidoreductase (pseudogene)" FT /pseudo FT CDS complement(813432..813959) FT /class="VI" FT /colour=8 FT /gene="ML0678" FT /ml_unique FT /note="Unknown function. Previously sequenced as TR:O32876 FT (EMBL:Z98271) (175 aa); Fasta score E(): 0, 99.4% identity FT in 175 aa overlap." FT /product="hypothetical protein" FT CDS 814145..814372 FT /class="VI" FT /colour=8 FT /gene="ML0679" FT /ml_unique FT /note="Unknown function." FT /product="hypothetical protein" FT CDS 814992..816275 FT /class="IV.B.2" FT /colour=0 FT /gene="ML0680" FT /note="Possible pseudogene similar to M. tuberculosis FT REP-family proteins (Best blastx score 392)" FT /product="REP-family protein (pseudogene)" FT /pseudo FT CDS complement(816353..817055) FT /class="V" FT /colour=0 FT /gene="ML0681" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3342 (Best blastx score 374)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(817109..818257) FT /class="I.D.2" FT /colour=7 FT /gene="metA" FT /gene="ML0682" FT /note="Similar to M. tuberculosis metA, Rv3341, homoserine FT o-acetyltransferase, TR:O53391 (EMBL:AL123456) (379 aa); FT Fasta score E(): 0, 85.0% identity in 380 aa overlap. FT Similar to many e.g. Leptospira meyeri metX, homoserine FT o-acetyltransferase, TR:P94891 (EMBL:Y10744) (379 aa); FT Fasta score E(): 0, 36.4% identity in 382 aa overlap. FT Previously sequenced as TR:O32874 (EMBL:Z98271) (382 aa); FT Fasta score E(): 0, 100.0% identity in 382 aa overlap. FT Contains Pfam match to entry PF00561 abhydrolase, FT alpha/beta hydrolase fold." FT /product="putative homoserine o-acetyltransferase" FT /tb_orthologue="metA" FT misc_feature complement(817154..817972) FT /colour=0 FT /note="Pfam match to entry PF00561 abhydrolase, alpha/beta FT hydrolase fold, score 36.40, E-value 6.6e-07" FT CDS complement(818272..819512) FT /class="I.D.2" FT /colour=0 FT /gene="metC" FT /gene="ML0683" FT /note="Possible pseudogene of M. tuberculosis orthologue FT metC (Best blastx score 582)" FT /product="cystathionine [beta]-lyase (pseudogene)" FT /pseudo FT CDS 819971..821100 FT /class="I.B.3" FT /colour=0 FT /gene="icd1" FT /gene="ML0684" FT /note="Possible pseudogene of M. tuberculosis orthologue FT icd1 (Best blastx score 480)" FT /product="isocitrate dehydrogenase (pseudogene)" FT /pseudo FT CDS complement(821179..822090) FT /class="V" FT /colour=10 FT /gene="ML0685" FT /note="Similar to M. tuberculosis Rv3337, conserved FT hypothetical protein, TR:O53387 (EMBL:AL123456) (82 aa); FT Fasta score E(): 2.8e-17, 74.3% identity in 70 aa overlap FT and Rv3338, conserved hypothetical protein, TR:O53388 FT (EMBL:AL123456) (214 aa); Fasta score E(): 0, 65.9% FT identity in 214 aa overlap which are similar to the FT N-terminus and remainder respectively. Show weak FT similarity to various hydrolases e.g. Rhodococcus sp. FT etbD1, aromatic compound hydrolase, TR:O82828 FT (EMBL:AB004320) (274 aa); Fasta score E(): 1.8e-05, 25.8% FT identity in 267 aa overlap. Previously sequenced as FT TR:Q49926 (EMBL:U00022) (303 aa); Fasta score E(): 0, FT 99.7% identity in 303 aa overlap. Contains Pfam match to FT entry PF00561 abhydrolase, alpha/beta hydrolase fold." FT /product="putative hydrolase" FT /tb_orthologue="Rv3338" FT misc_feature complement(821224..821886) FT /colour=0 FT /note="Pfam match to entry PF00561 abhydrolase, alpha/beta FT hydrolase fold, score 38.00, E-value 2.1e-07" FT CDS 822126..823157 FT /EC_number="6.1.1.2" FT /class="II.A.3" FT /colour=2 FT /gene="trpS" FT /gene="ML0686" FT /note="Similar to M. tuberculosis trpS, Rv3336c, FT tryptophanyl-tRNA synthetase, SW:SYW_MYCTU (O53386) (336 FT aa); Fasta score E(): 0, 83.8% identity in 339 aa overlap. FT Similar to many e.g. Escherichia coli trpS, tryptophanyl FT tRNA synthetase, SW:SYW_ECOLI (P00954) (334 aa); Fasta FT score E(): 0, 49.6% identity in 335 aa overlap. Previously FT sequenced as SW:SYW_MYCLE (Q49901) (343 aa); Fasta score FT E(): 0, 100.0% identity in 343 aa overlap. Contains Pfam FT match to entry PF00579 tRNA-synt_1b, tRNA synthetases FT class I (W and Y). Contains PS00017 ATP/GTP-binding site FT motif A (P-loop). Contains PS00178 Aminoacyl-transfer RNA FT synthetases class-I signature." FT /product="tryptophanyl tRNA synthetase" FT /tb_orthologue="trpS" FT misc_feature 822153..822839 FT /colour=0 FT /note="Pfam match to entry PF00579 tRNA-synt_1b, tRNA FT synthetases class I (W and Y), score 334.40, E-value FT 1.3e-96" FT misc_feature 822171..822203 FT /colour=8 FT /note="PS00178 Aminoacyl-transfer RNA synthetases class-I FT signature" FT misc_feature 823086..823109 FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT CDS 823169..824110 FT /class="II.C.4" FT /colour=3 FT /gene="ML0687" FT /note="Similar to M. tuberculosis Rv3335c, conserved FT hypothetical protein, TR:O53385 (EMBL:AL123456) (289 aa); FT Fasta score E(): 0, 68.8% identity in 288 aa overlap. FT Similar to bacterial hypothetical proteins e.g. FT Escherichia coli yhjD, hypothetical protein, SW:YHJD_ECOLI FT (P37642) (337 aa); Fasta score E(): 5e-30, 34.9% identity FT in 278 aa overlap. Previously sequenced as TR:Q49909 FT (EMBL:U00022) (313 aa); Fasta score E(): 0, 100.0% FT identity in 313 aa overlap. Contains hydrophobic, possible FT membrane-spanning regions." FT /product="putative membrane protein" FT /tb_orthologue="Rv3335c" FT CDS complement(824241..825376) FT /class="II.C.3" FT /colour=0 FT /gene="nagA" FT /gene="ML0688" FT /note="Possible pseudogene of M. tuberculosis orthologue FT nagA (Best blastx score 253)" FT /product="N-acetylglucosamine-6-P-deacetylase FT (pseudogene)" FT /pseudo FT CDS complement(825391..826048) FT /class="IV.H" FT /colour=0 FT /gene="ML0689" FT /note="Possible pseudogene of M. tuberculosis paralog glmS FT (Best blastx score 90)" FT /product="putative aminotransferase (pseudogene)" FT /pseudo FT CDS complement(826327..827763) FT /class="III.A.3" FT /colour=0 FT /gene="sugI" FT /gene="ML0690" FT /note="Possible pseudogene of M. tuberculosis orthologue FT sugI (Best blastx score 360)" FT /product="probable sugar transport protein (pseudogene)" FT /pseudo FT CDS complement(827877..829112) FT /class="II.C.3" FT /colour=3 FT /gene="ML0691" FT /note="Similar to M. tuberculosis Rv3330, putative FT penicillin binding protein, TR:O53380 (EMBL:AL123456) (405 FT aa); Fasta score E(): 0, 77.2% identity in 416 aa overlap. FT Similar to PBP/D-alanyl-D-alanine carboxypeptidases e.g. FT Bacillus subtilis dacB, penicillin-binding protein 5* FT precursor, SW:DACB_BACSU (P35150) (382 aa); Fasta score FT E(): 2.2e-15, 31.3% identity in 243 aa overlap. Previously FT sequenced as TR:Q49917 (EMBL:U00022) (228 aa); Fasta score FT E(): 0, 100.0% identity in 228 aa overlap. Contains a FT probable N-terminal signal sequence. Contains Pfam match FT to entry PF00768 Peptidase_S11, D-alanyl-D-alanine FT carboxypeptidase." FT /product="putative D-alanyl-D-alanine carboxypeptidase" FT /tb_orthologue="Rv3330" FT misc_feature complement(828078..828842) FT /colour=0 FT /note="Pfam match to entry PF00768 Peptidase_S11, FT D-alanyl-D-alanine carboxypeptidase, score 179.80, E-value FT 4.3e-50" FT CDS complement(830042..831172) FT /class="IV.H" FT /colour=0 FT /gene="ML0692" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3329 (Best blastx score 840)" FT /product="probable aminotransferase (pseudogene)" FT /pseudo FT CDS 831183..831978 FT /class="II.A.7" FT /colour=0 FT /gene="sigJ" FT /gene="ML0693" FT /note="Possible pseudogene of M. tuberculosis orthologue FT sigJ (Best blastx score 342)" FT /product="similar to SigI, ECF family (pseudogene)" FT /pseudo FT CDS complement(832026..832536) FT /class="IV.B.1.c" FT /colour=0 FT /gene="ML0694" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3327 (Best blastx score 175)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS 833165..833501 FT /class="V" FT /colour=0 FT /gene="ML0695" FT /note="Possible pseudogene similar to M. tuberculosis FT Rv2893 (Best blastx score 106)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(833872..834666) FT /EC_number="1.3.99.1" FT /class="I.B.3" FT /colour=7 FT /gene="sdhB" FT /gene="ML0696" FT /note="Similar to M. tuberculosis sdhB, Rv3319, succinate FT dehydrogenase subunit B, TR:O53371 (EMBL:AL123456) (263 FT aa); Fasta score E(): 0, 89.8% identity in 264 aa overlap. FT Similar to many e.g. Escherichia coli sdhB, succinate FT dehydrogenase iron-sulfur protein, SW:DHSB_ECOLI (P07014) FT (238 aa); Fasta score E(): 0, 42.9% identity in 240 aa FT overlap. Previously sequenced as TR:Q49916 (EMBL:U00022) FT (264 aa); Fasta score E(): 0, 100.0% identity in 264 aa FT overlap. Contains Pfam match to entry PF00111 fer2, 2Fe-2S FT iron-sulfur cluster binding domains. Contains PS00198 FT 4Fe-4S ferredoxins, iron-sulfur binding region signature." FT /product="succinate dehydrogenase iron-sulfur protein" FT /tb_orthologue="sdhB" FT misc_feature complement(834103..834138) FT /colour=8 FT /note="PS00198 4Fe-4S ferredoxins, iron-sulfur binding FT region signature" FT misc_feature complement(834349..834516) FT /colour=0 FT /note="Pfam match to entry PF00111 fer2, 2Fe-2S FT iron-sulfur cluster binding domains, score 5.80, E-value FT 1.8" FT CDS complement(834666..836420) FT /EC_number="1.3.99.1" FT /class="I.B.3" FT /colour=7 FT /gene="sdhA" FT /gene="ML0697" FT /note="Similar to M. tuberculosis sdhA, Rv3318, succinate FT dehydrogenase subunit A, TR:O53370 (EMBL:AL123456) (590 FT aa); Fasta score E(): 0, 92.5% identity in 590 aa overlap. FT Similar to many e.g. Escherichia coli sdhA, succinate FT dehydrogenase flavoprotein subunit, SW:DHSA_ECOLI (P10444) FT (588 aa); Fasta score E(): 0, 48.6% identity in 589 aa FT overlap. Previously sequenced as TR:Q49924 (EMBL:U00022) FT (401 aa); Fasta score E(): 0, 97.2% identity in 395 aa FT overlap. Contains Pfam match to entry PF00890 FT FAD_binding_2, FAD binding domain. Contains PS00504 FT Fumarate reductase / succinate dehydrogenase FAD-binding FT site." FT /product="succinate dehydrogenase flavoprotein subunit" FT /tb_orthologue="sdhA" FT misc_feature complement(835116..836105) FT /colour=0 FT /note="Pfam match to entry PF00890 FAD_binding_2, FAD FT binding domain, score 590.20, E-value 1.2e-173" FT misc_feature complement(836271..836300) FT /colour=8 FT /note="PS00504 Fumarate reductase / succinate FT dehydrogenase FAD-binding site" FT CDS complement(836441..836932) FT /class="I.B.3" FT /colour=7 FT /gene="sdhD" FT /gene="ML0698" FT /note="Similar to M. tuberculosis sdhD, Rv3317, putative FT membrane anchor of succinate dehydrogenase, TR:O53369 FT (EMBL:AL123456) (144 aa); Fasta score E(): 0, 85.2% FT identity in 142 aa overlap. Shows weak similarity to e.g. FT Rickettsia prowazekii sdhD, succinate dehydrogenase FT hydrophobic membrane anchor protein, SW:DHSD_RICPR FT (P41086) (125 aa); Fasta score E(): 0.24, 26.7% identity FT in 105 aa overlap. Previously sequenced as TR:Q49915 FT (EMBL:U00022) (163 aa); Fasta score E(): 0, 100.0% FT identity in 163 aa overlap.Contains hydrophobic, possible FT membrane-spanning regions." FT /product="putative succinate dehydrogenase hydrophobic FT membrane anchor protei" FT /tb_orthologue="sdhD" FT CDS complement(836929..837390) FT /class="I.B.3" FT /colour=7 FT /gene="sdhC" FT /gene="ML0699" FT /note="Similar to M. tuberculosis sdhC, Rv3316, putative FT membrane anchor of succinate dehydrogenase, TR:O53368 FT (EMBL:AL123456) (112 aa); Fasta score E(): 0, 88.4% FT identity in 112 aa overlap. Similar to many e.g. FT Escherichia coli sdhC, succinate dehydrogenase cytochrome FT B-556, SW:DHSC_ECOLI (P10446) (129 aa); Fasta score E(): FT 0.074, 24.3% identity in 136 aa overlap. Previously FT sequenced as TR:Q49919 (EMBL:U00022) (244 aa); Fasta score FT E(): 0, 100.0% identity in 153 aa overlap. Contains Pfam FT match to entry PF01127 Sdh_cyt, Succinate dehydrogenase FT cytochrome b subunit." FT /product="putative succinate dehydrogenase cytochrome FT B-556 subunit" FT /tb_orthologue="sdhC" FT misc_feature complement(837001..837330) FT /colour=0 FT /note="Pfam match to entry PF01127 Sdh_cyt, Succinate FT dehydrogenase cytochrome b subunit, score -28.10, E-value FT 0.051" FT CDS 837679..838767 FT /class="I.F.4" FT /colour=7 FT /gene="add" FT /gene="ML0700" FT /note="Similar to M. tuberculosis add, Rv3313c, probable FT adenosine deaminase, TR:O53365 (EMBL:AL123456) (365 aa); FT Fasta score E(): 0, 88.2% identity in 356 aa overlap. FT Similar to many e.g. Escherichia coli add, adenosine FT deaminase, SW:ADD_ECOLI (P22333) (333 aa); Fasta score FT E(): 6.8e-19, 29.7% identity in 340 aa overlap. Previously FT sequenced as TR:Q49907 (EMBL:U00022) (332 aa); Fasta score FT E(): 0, 99.7% identity in 332 aa overlap. Contains Pfam FT match to entry PF00962 A_deaminase, Adenosine/AMP FT deaminase." FT /product="putative adenosine deaminase" FT /tb_orthologue="add" FT misc_feature 837691..838731 FT /colour=0 FT /note="Pfam match to entry PF00962 A_deaminase, FT Adenosine/AMP deaminase, score 395.60, E-value 4.8e-115" FT CDS 838834..839852 FT /class="II.C.4" FT /colour=0 FT /gene="ML0701" FT /note="Possible pseudogene of M. tuberculosis paralog FT Rv1481 (Best blastx score 222)" FT /product="possible membrane protein (pseudogene)" FT /pseudo FT CDS complement(839856..840813) FT /class="II.C.4" FT /colour=0 FT /gene="ML0702" FT /note="Possible pseudogene of M. tuberculosis paralog FT Rv1481 (Best blastx score 92)" FT /product="possible membrane protein (pseudogene)" FT /pseudo FT CDS complement(840882..842153) FT /class="V" FT /colour=10 FT /gene="ML0703" FT /note="Similar to M. tuberculosis Rv3311, conserved FT hypothetical protein, TR:O53362 (EMBL:AL123456) (420 aa); FT Fasta score E(): 0, 77.5% identity in 423 aa overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv3311" FT CDS complement(842232..843069) FT /class="IV.I" FT /colour=0 FT /gene="ML0704" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3310 (Best blastx score 491)" FT /product="probable acid phosphatase (pseudogene)" FT /pseudo FT CDS 843205..843831 FT /class="I.F.4" FT /colour=0 FT /gene="upp" FT /gene="ML0705" FT /note="Possible pseudogene of M. tuberculosis orthologue FT upp (Best blastx score 251)" FT /product="uracil phophoribosyltransferase (pseudogene)" FT /pseudo FT CDS complement(843847..845463) FT /class="I.F.4" FT /colour=7 FT /gene="pmmB" FT /gene="ML0706" FT /note="Similar to M. tuberculosis pmmB, putative FT phosphomannomutase, TR:O53360 (EMBL:AL123456) (534 aa); FT Fasta score E(): 0, 76.8% identity in 538 aa overlap. FT Similar to many putative phospho-sugar mutases and to FT Streptococcus pneumoniae pgm, phosphoglucomutase involved FT in type 3 capsule production and basic metabolism, FT TR:Q9RP94 (EMBL:AF165218) (572 aa); Fasta score E(): FT 1.4e-19, 32.6% identity in 549 aa overlap. Previously FT sequenced as TR:Q49913 (EMBL:U00022) (534 aa); Fasta score FT E(): 0, 99.8% identity in 534 aa overlap. Contains Pfam FT match to entry PF00408 PGM_PMM, FT Phosphoglucomutase/phosphomannomutase. Contains PS00710 FT Phosphoglucomutase and phosphomannomutase phosphoserine FT signature." FT /note="Shows weak similarity to ML0366 and ML0763" FT /product="putative phospho-sugar mutase" FT /tb_orthologue="pmmB" FT misc_feature complement(843916..845355) FT /colour=0 FT /note="Pfam match to entry PF00408 PGM_PMM, FT Phosphoglucomutase/phosphomannomutase, score 109.30, FT E-value 7.4e-29" FT misc_feature complement(845014..845058) FT /colour=8 FT /note="PS00710 Phosphoglucomutase and phosphomannomutase FT phosphoserine signature" FT CDS complement(845460..846266) FT /EC_number="2.4.2.1" FT /class="I.F.4" FT /colour=7 FT /gene="deoD" FT /gene="ML0707" FT /note="Similar to M. tuberculosis deoD, Rv3307, probable FT purine nucleoside phosphorylase, SW:PUNA_MYCTU (O53359) FT (268 aa); Fasta score E(): 0, 82.1% identity in 262 aa FT overlap. Similar to many e.g. Bacillus stearothermophilus FT deoD, purine nucleoside phosphorylase I, SW:PUNA_BACST FT (P77834) (274 aa); Fasta score E(): 1.3e-16, 43.1% FT identity in 246 aa overlap. Previously sequenced as FT SW:PUNA_MYCLE (P46862) (268 aa); Fasta score E(): 0, 99.6% FT identity in 268 aa overlap. Contains Pfam match to entry FT PF00896 Mtap_PNP, phosphorylases family 2. Contains FT PS01240 Purine and other phosphorylases family 2 FT signature." FT /product="putative purine nucleoside phosphorylase" FT /tb_orthologue="deoD" FT misc_feature complement(845574..846179) FT /colour=0 FT /note="Pfam match to entry PF00896 Mtap_PNP, FT phosphorylases family 2, score 357.90, E-value 1e-103" FT misc_feature complement(845895..846020) FT /colour=8 FT /note="PS01240 Purine and other phosphorylases family 2 FT signature" FT CDS 846400..847610 FT /class="II.B.3" FT /colour=0 FT /gene="amiB" FT /gene="ML0708" FT /note="Possible pseudogene of M. tuberculosis orthologue FT amiB (Best blastx score 355)" FT /product="probable aminohydrolase (pseudogene)" FT /pseudo FT CDS 847645..848747 FT /class="II.B.3" FT /colour=0 FT /gene="amiA" FT /gene="ML0709" FT /note="Possible pseudogene of M. tuberculosis orthologue FT amiA (Best blastx score 489)" FT /product="probable aminohydrolase (pseudogene)" FT /pseudo FT CDS 848943..849542 FT /class="V" FT /colour=0 FT /gene="ML0710" FT /note="Possible pseudogene of M. tuberculosis paralog FT Rv2248 (Best blastx score 255)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(849828..850307) FT /class="V" FT /colour=10 FT /gene="ML0711" FT /note="Similar to M. tuberculosis Rv3304, conserved FT hypothetical protein, TR:O53356 (EMBL:AL123456) (159 aa); FT Fasta score E(): 0, 91.8% identity in 159 aa overlap. FT Shows weak similarity to Azotobacter vinelandii FT hypothetical protein, TR:Q44546 (EMBL:M23528) (183 aa); FT Fasta score E(): 1.3e-06, 30.1% identity in 146 aa overlap FT and Homo sapiens WUGSC:H_DJ0777O23.1, hypothetical FT protein, TR:O75223 (EMBL:AC005154) (188 aa); Fasta score FT E(): 4.9e-06, 26.8% identity in 149 aa overlap. Previously FT sequenced as TR:Q49927 (EMBL:U00022) (174 aa); Fasta score FT E(): 0, 98.7% identity in 149 aa overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv3304" FT CDS 850436..851854 FT /class="I.B.2" FT /colour=0 FT /gene="lpdA" FT /gene="ML0712" FT /note="Possible pseudogene of M. tuberculosis orthologue FT lpdA (Best blastx score 410)" FT /product="dihydrolipoamide dehydrogenase (pseudogene)" FT /pseudo FT RBS 851933..851937 FT /note="possible RBS" FT CDS 851948..853705 FT /class="I.B.6.a" FT /colour=7 FT /gene="glpD" FT /gene="ML0713" FT /note="Similar to M. tuberculosis glpD2, Rv3302c, FT glycerol-3-phosphate dehydrogenase, TR:O07168 FT (EMBL:AL123456) (585 aa); Fasta score E(): 0, 90.8% FT identity in 584 aa overlap. Similar to many e.g. FT Escherichia coli, glpD, glycerol-3-phosphate FT dehydrogenase, SW:GLPD_ECOLI (P13035) (501 aa); Fasta FT score E(): 0, 35.2% identity in 492 aa overlap. Also FT similar to Brucella abortus eriB, erythritol phosphate FT dehydrogenase, TR:Q9ZB31 (EMBL:U57100) (502 aa); Fasta FT score E(): 0, 35.4% identity in 483 aa overlap. Previously FT sequenced as SW:GLPD_MYCLE (P53435) (585 aa); Fasta score FT E(): 0, 99.8% identity in 585 aa overlap. Contains Pfam FT match to entry PF01224 FAD_Gly3P_dh, FAD-dependent FT glycerol-3-phosphate dehydrogenase. Contains PS00978 FT FAD-dependent glycerol-3-phosphate dehydrogenase signature FT 2. Contains PS00977 FAD-dependent glycerol-3-phosphate FT dehydrogenase signature 1." FT /product="putative glycerol-3-phosphate dehydrogenase" FT /tb_orthologue="glpD2" FT misc_feature 852044..853639 FT /colour=0 FT /note="Pfam match to entry PF01224 FAD_Gly3P_dh, FT FAD-dependent glycerol-3-phosphate dehydrogenase, score FT 994.40, E-value 2.7e-295" FT misc_feature 852068..852121 FT /colour=8 FT /note="PS00977 FAD-dependent glycerol-3-phosphate FT dehydrogenase signature 1" FT misc_feature 853100..853132 FT /colour=8 FT /note="PS00978 FAD-dependent glycerol-3-phosphate FT dehydrogenase signature 2" FT CDS 853693..854513 FT /class="I.G.9" FT /colour=0 FT /gene="ML0714" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3300c (Best blastx score 559)" FT /product="probable deaminase, riboflavin synthesis FT (pseudogene)" FT /pseudo FT CDS 854776..855690 FT /class="II.C.2" FT /colour=3 FT /gene="lpqC" FT /gene="ML0715" FT /note="Similar to M. tuberculosis lpqC, Rv3298c, FT hypothetical protein, TR:P96903 (EMBL:AL123456) (304 aa); FT Fasta score E(): 0, 72.0% identity in 300 aa overlap. FT Shows weak similarity to hydrolases e.g. Piromyces equi FT estA, cinnamoyl ester hydrolase, TR:Q9Y871 (EMBL:AF164516) FT (536 aa); Fasta score E(): 4.8e-12, 27.9% identity in 265 FT aa overlap and Pseudomonas lemoignei phaZ1, secreted FT poly(3-hydroxyalkanoate) depolymerase C precursor, FT SW:PHA1_PSELE (P52090) (414 aa); Fasta score E(): 2.5e-06, FT 25.6% identity in 320 aa overlap. Previously sequenced as FT TR:Q49658 (EMBL:U00012) (302 aa); Fasta score E(): 0, FT 99.7% identity in 302 aa overlap. Contains a possible FT N-terminal signal sequence." FT /product="putative secreted hydrolase" FT /tb_orthologue="lpqC" FT CDS complement(856124..859813) FT /class="II.A.5" FT /colour=0 FT /gene="lhr" FT /gene="ML0716" FT /note="Possible pseudogene of M. tuberculosis orthologue FT lhr (Best blastx score 824)" FT /product="ATP-dependent helicase (pseudogene)" FT /pseudo FT CDS complement(859917..860588) FT /class="I.J.1" FT /colour=9 FT /gene="ML0717" FT /note="Similar to M. tuberculosis Rv3295, putative FT TetR-family transcriptional regulator, TR:P96900 FT (EMBL:AL123456) (221 aa); Fasta score E(): 0, 85.5% FT identity in 220 aa overlap. Similar to many putative FT transcriptional regulators. Similar to Streptomyces tendae FT tarA, gamma-butyrolactone-binding protein, TR:Q9RPK9 FT (Expect = 5.0e-05). Previously sequenced as TR:Q49662 FT (EMBL:U00012) (242 aa); Fasta score E(): 0, 100.0% FT identity in 223 aa overlap. Contains a probable FT helix-turn-helix motif at aa 33-54 (Score 1535, SD +4.42) FT Contains Pfam match to entry PF00440 tetR, Bacterial FT regulatory proteins, tetR family." FT /product="putative TetR-family transcriptional regulator" FT /tb_orthologue="Rv3295" FT misc_feature complement(860400..860540) FT /colour=0 FT /note="Pfam match to entry PF00440 tetR, Bacterial FT regulatory proteins, tetR family, score 61.40, E-value FT 2e-14" FT CDS complement(860656..861952) FT /class="I.B.7" FT /colour=0 FT /gene="aldB" FT /gene="ML0718" FT /note="Possible pseudogene of M. tuberculosis orthologue FT aldB (Best blastx score 421)" FT /product="aldehyde dehydrogenase (pseudogene)" FT /pseudo FT CDS complement(862129..863299) FT /class="V" FT /colour=0 FT /gene="ML0719" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3292 (Best blastx score 499)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS 863378..863778 FT /class="I.J.1" FT /colour=0 FT /gene="ML0720" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3291c (Best blastx score 358)" FT /product="transcriptional regulator (Lrp/AsnC family) FT (pseudogene)" FT /pseudo FT CDS 863877..865225 FT /class="IV.D" FT /colour=0 FT /gene="lat" FT /gene="ML0721" FT /note="Possible pseudogene of M. tuberculosis orthologue FT lat (Best blastx score 509)" FT /product="lysine-[epsilon] aminotransferase (pseudogene)" FT /pseudo FT CDS 865371..865700 FT /class="V" FT /colour=0 FT /gene="ML0722" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3289c (Best blastx score 261)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS 865725..866096 FT /class="V" FT /colour=0 FT /gene="ML0723" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3288c (Best blastx score 210)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS 866036..866821 FT /class="II.A.7" FT /colour=0 FT /gene="rsbW" FT /gene="ML0724" FT /note="Possible pseudogene of M. tuberculosis orthologue FT rsbW (Best blastx score 286)" FT /product="anti-sigma B factor (pseudogene)" FT /pseudo FT CDS 866917..867685 FT /class="II.A.7" FT /colour=0 FT /gene="sigF" FT /gene="ML0725" FT /note="Possible pseudogene of M. tuberculosis orthologue FT sigF (Best blastx score 406)" FT /product="ECF subfamily sigma subunit (pseudogene)" FT /pseudo FT CDS complement(867696..869492) FT /EC_number="6.3.4.14" FT /class="I.H.1" FT /colour=1 FT /gene="bccA" FT /gene="ML0726" FT /note="Similar to M. tuberculosis accA3, Rv3285, FT acetyl/propionyl CoA carboxylase [alpha] subunit, FT TR:P96890 (EMBL:AL123456) (600 aa); Fasta score E(): 0, FT 88.7% identity in 601 aa overlap. Previously sequenced as FT SW:BCCA_MYCLE (P46392) (598 aa); Fasta score E(): 0, 99.7% FT identity in 598 aa overlap. Contains Pfam match to entry FT PF00364 biotin_lipoyl, Biotin-requiring enzymes. Contains FT Pfam match to entry PF00289 CPSase_L_chain, FT Carbamoyl-phosphate synthase (CPSase). Contains PS00867 FT Carbamoyl-phosphate synthase subdomain signature 2. FT Contains PS00866 Carbamoyl-phosphate synthase subdomain FT signature 1. Contains PS00188 Biotin-requiring enzymes FT attachment site." FT /product="acetyl/propionyl CoA carboxylase [alpha] FT subunit" FT /tb_orthologue="accA3" FT misc_feature complement(867702..867905) FT /colour=0 FT /note="Pfam match to entry PF00364 biotin_lipoyl, FT Biotin-requiring enzymes, score 88.00, E-value 1.9e-22" FT misc_feature complement(867780..867833) FT /colour=8 FT /note="PS00188 Biotin-requiring enzymes attachment site" FT misc_feature complement(868362..869462) FT /colour=0 FT /note="Pfam match to entry PF00289 CPSase_L_chain, FT Carbamoyl-phosphate synthase (CPSase), score 668.20, FT E-value 4.1e-197" FT misc_feature complement(868593..868616) FT /colour=8 FT /note="PS00867 Carbamoyl-phosphate synthase subdomain FT signature 2" FT misc_feature complement(868971..869015) FT /colour=8 FT /note="PS00866 Carbamoyl-phosphate synthase subdomain FT signature 1" FT RBS complement(869497..869502) FT /note="possible RBS" FT CDS complement(869612..870011) FT /class="V" FT /colour=0 FT /gene="ML0727" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3284 (Best blastx score 203)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(870028..870918) FT /EC_number="2.8.1.1" FT /class="I.C.5" FT /colour=7 FT /gene="sseA" FT /gene="ML0728" FT /note="Similar to M. tuberculosis sseA, Rv3283, putative FT thiosulfate sulfurtransferase, SW:THT2_MYCTU (P96888) (297 FT aa); Fasta score E(): 0, 83.4% identity in 296 aa overlap. FT Similar to many e.g. Azotobacter vinelandii rhdA, FT thiosulfate sulfurtransferase, SW:THTR_AZOVI (P52197) (271 FT aa); Fasta score E(): 1.1e-18, 33.5% identity in 269 aa FT overlap. Previously sequenced as SW:THT2_MYCLE (P46700) FT (296 aa); Fasta score E(): 0, 99.7% identity in 296 aa FT overlap. Contains 2 Pfam matches to entry PF00581 FT Rhodanese, Rhodanese-like domain. Contains PS00380 FT Rhodanese signature 1. Contains PS00683 Rhodanese FT C-terminal signature." FT /note="Similar to ML2198" FT /product="putative thiosulfate sulfurtransferase" FT /tb_orthologue="sseA" FT misc_feature complement(870079..870438) FT /colour=0 FT /note="Pfam match to entry PF00581 Rhodanese, FT Rhodanese-like domain, score 108.90, E-value 9.9e-29" FT misc_feature complement(870088..870120) FT /colour=8 FT /note="PS00683 Rhodanese C-terminal signature" FT misc_feature complement(870523..870855) FT /colour=0 FT /note="Pfam match to entry PF00581 Rhodanese, FT Rhodanese-like domain, score 109.10, E-value 8.5e-29" FT misc_feature complement(870745..870780) FT /colour=8 FT /note="PS00380 Rhodanese signature 1" FT RBS complement(870934..870939) FT /note="possible RBS" FT CDS complement(871119..871760) FT /class="V" FT /colour=10 FT /gene="ML0729" FT /note="Similar to M. tuberculosis Rv3282, conserved FT hypothetical protein, TR:P96887 (EMBL:AL123456) (222 aa); FT Fasta score E(): 0, 68.5% identity in 213 aa overlap. FT Similar to maf-family proteins e.g. Bacillus subtilis maf, FT protein involved in septum formation, SW:MAF_BACSU FT (Q02169) (189 aa); Fasta score E(): 4.8e-08, 29.1% FT identity in 196 aa overlap. Also similar to the N-terminal FT half of Homo sapiens asmtl, acetylserotonin FT methytransferase-like gene, TR:O95671 (EMBL:Y15521) (629 FT aa); Fasta score E(): 2.1e-09, 31.6% identity in 193 aa FT overlap. Previously sequenced as TR:Q49670 (EMBL:U00012) FT (213 aa); Fasta score E(): 0, 100.0% identity in 213 aa FT overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv3282" FT CDS complement(871757..872011) FT /class="VI" FT /colour=10 FT /gene="ML0730" FT /note="Similar to Mycobacterium tuberculosis hypothetical FT 19.0 kda protein RV3281 OR MTCY71.21 TR:P96886 FT (EMBL:Z92771) fasta scores: E(): 2.4e-22, 69.0% id in 87 FT aa. Previously sequenced as TR:Q49671 (EMBL:U00012). FT Contains Pfam match to entry PF01039 Carboxyl_trans, FT Carboxyl transferase domain." FT /tb_orthologue="Rv3281" FT CDS complement(872008..873657) FT /EC_number="6.4.1.3" FT /class="I.H.1" FT /colour=1 FT /gene="accD5" FT /gene="ML0731" FT /note="Similar to Mycobacterium tuberculosis pccb PCCB OR FT RV3280 OR MTCY71.20 TR:P96885 (EMBL:Z92771) fasta scores: FT E(): 0, 88.7% id in 549 aa, and to Saccharopolyspora FT erythraea propionyl-CoA carboxylase beta chain PCCB FT SW:PCCB_SACER (P53003) fasta scores: E(): 0, 69.1% id in FT 530 aa. Previously sequenced as SW:PCCB_MYCLE (P53002). FT Contains Pfam match to entry PF01039 Carboxyl_trans, FT Carboxyl transferase domain." FT /note="Similar to ML0102 and ML1657" FT /product="acetyl/propionyl CoA carboxylase [beta] subunit" FT /tb_orthologue="accD5" FT misc_feature complement(872011..873555) FT /colour=0 FT /note="Pfam match to entry PF01039 Carboxyl_trans, FT Carboxyl transferase domain, score 915.00, E-value FT 2.1e-271" FT RBS 873710..873714 FT /note="possible RBS" FT CDS 873720..874544 FT /class="I.G.1" FT /colour=7 FT /gene="birA" FT /gene="ML0732" FT /note="Similar to Mycobacterium tuberculosis birA OR FT RV3279C OR MTCY71.19C TR:P96884 (EMBL:Z92771) fasta FT scores: E(): 0, 71.2% id in 271 aa, and to Escherichia FT coli birA bifunctional protein [includes: biotin operon FT repressor; biotin--[acetyl-coa-carboxylase] synthetase FT birA OR bioR OR dhbB SW:BIRA_ECOLI (P06709) fasta scores: FT E(): 2e-10, 31.3% id in 256 aa. Lacks the N-terminal FT helix-turn-helix domain, so may not act as a repressor. FT Contains Pfam match to entry PF01317 BPL, Biotin protein FT ligase." FT /product="biotin apo-protein ligase" FT /tb_orthologue="birA" FT misc_feature 873792..874538 FT /colour=0 FT /note="Pfam match to entry PF01317 BPL, Biotin protein FT ligase, score 265.60, E-value 6.4e-76" FT CDS 874677..875195 FT /class="II.C.4" FT /colour=3 FT /gene="ML0733" FT /note="Similar to Mycobacterium tuberculosis hypothetical FT 19.8 kda protein RV3278C OR MTCY71.18C TR:P96883 FT (EMBL:Z92771) fasta scores: E(): 0, 83.1% id in 172 aa. FT Previously sequenced as TR:Q49672 (EMBL:U00012). Contains FT hydrophobic, possible membrane-spanning regions." FT /product="putative membrane protein" FT /tb_orthologue="Rv3278c" FT CDS complement(875150..875836) FT /class="II.C.4" FT /colour=3 FT /gene="ML0734" FT /note="Similar to Mycobacterium tuberculosis hypothetical FT 30.1 kDa protein RV3277 OR MTCY71.17 TR:P96882 FT (EMBL:Z92771) fasta scores: E(): 0, 84.6% id in 228 aa. FT Previously sequenced as TR:Q49673 (EMBL:U00012). Contains FT hydrophobic, possible membrane-spanning regions." FT /product="putative membrane protein" FT /tb_orthologue="Rv3277" FT CDS 875924..877243 FT /EC_number="4.1.1.21" FT /class="I.F.1" FT /colour=7 FT /gene="purK" FT /gene="ML0735" FT /note="Similar to Mycobacterium tuberculosis FT phosphoribosylaminoimidazole carboxylase atpase subunit FT purK OR RV3276C OR MTCY71.16C SW:PURK_MYCTU (P96881) fasta FT scores: E(): 0, 76.1% id in 444 aa, and to Corynebacterium FT ammoniagenes phosphoribosylaminoimidazole carboxylase FT ATPase subunit purK SW:PURK_CORAM (Q44678) fasta scores: FT E(): 0, 48.3% id in 404 aa. Previously sequenced as FT SW:PURK_MYCLE (P46701). Contains Pfam match to entry FT PF02222 ATP-grasp, ATP-grasp domain." FT /product="phosphoribosylaminoimidazole carboxylase ATPase" FT /tb_orthologue="purK" FT misc_feature 876047..876556 FT /colour=0 FT /note="Pfam match to entry PF02222 ATP-grasp, ATP-grasp FT domain, score 145.20, E-value 1.2e-39" FT RBS 877230..877234 FT /note="possible RBS" FT CDS 877243..877758 FT /EC_number="4.1.1.21" FT /class="I.F.1" FT /colour=7 FT /gene="purE" FT /gene="ML0736" FT /note="Similar to Mycobacterium tuberculosis FT phosphoribosylaminoimidazole carboxylase catalytic subunit FT purE or Rv3275c or MTCY71.15C SW:PUR6_MYCTU (P96880) (174 FT aa) fasta scores: E(): 0, 81.5% id in 168 aa and to FT Corynebacterium ammoniagenes phosphoribosylaminoimidazole FT carboxylase catalytic subunit purE SW:PUR6_CORAM (Q44679) FT (177 aa) fasta scores: E(): 0, 66.2% id in 148 aa. FT Previously sequenced as SW:PUR6_MYCLE (P46702). Contains FT Pfam match to entry PF00731 AIRC, AIR carboxylase." FT /product="phosphoribosylaminoimidazole carboxylase" FT /tb_orthologue="purE" FT misc_feature 877258..877701 FT /colour=0 FT /note="Pfam match to entry PF00731 AIRC, AIR carboxylase, FT score 322.30, E-value 5.6e-93" FT RBS 877842..877847 FT /note="possible RBS" FT CDS 877853..879022 FT /class="I.A.3" FT /colour=1 FT /gene="fadE25" FT /gene="ML0737" FT /note="Similar to Mycobacterium tuberculosis RV3274C OR FT MTCY71.14C, putative acyl-CoA dehydrogenase, TR:P96879 FT (EMBL:Z92771) fasta scores: E(): 0, 92.0% id in 389 aa, FT and to Streptomyces lividans fatty acid acyl-CoA FT dehydrogenase fadE TR:Q9RIQ5 (EMBL:AJ250495) fasta scores: FT E(): 0, 67.6% id in 383 aa. Previously sequenced as FT SW:ACD_MYCLE (P46703). Contains Pfam match to entry FT PF00441 Acyl-CoA_dh, Acyl-CoA dehydrogenase. Contains FT PS00073 Acyl-CoA dehydrogenases signature 2. Contains FT PS00072 Acyl-CoA dehydrogenases signature 1." FT /note="Similar to ML0660, ML0661 and shows weaker FT similarity to ML2563" FT /product="putative acyl-CoA dehydrogenase" FT /tb_orthologue="fadE25" FT misc_feature 877895..879007 FT /colour=0 FT /note="Pfam match to entry PF00441 Acyl-CoA_dh, Acyl-CoA FT dehydrogenase, score 798.10, E-value 3.4e-236" FT misc_feature 878249..878287 FT /colour=8 FT /note="PS00072 Acyl-CoA dehydrogenases signature 1" FT misc_feature 878888..878947 FT /colour=8 FT /note="PS00073 Acyl-CoA dehydrogenases signature 2" FT CDS complement(879312..879458) FT /class="III.A.4" FT /colour=0 FT /gene="pstA2" FT /gene="ML0738" FT /note="Possible pseudogene of M. tuberculosis orthologue FT pstA2 (Best blastx score 95)" FT /product="PstA component of phosphate uptake (pseudogene)" FT /pseudo FT CDS complement(879534..880518) FT /class="III.A.4" FT /colour=0 FT /gene="pstC" FT /gene="ML0739" FT /note="Possible pseudogene of M. tuberculosis orthologue FT pstC (Best blastx score 346)" FT /product="PstC component of phosphate uptake (pseudogene)" FT /pseudo FT CDS complement(881071..881654) FT /class="III.A.4" FT /colour=0 FT /gene="phoS1" FT /gene="ML0740" FT /note="Possible pseudogene of M. tuberculosis orthologue FT phoS1 (Best blastx score 238)" FT /product="PstS component of phosphate uptake (pseudogene)" FT /pseudo FT CDS complement(881783..882588) FT /class="III.A.4" FT /colour=0 FT /gene="pstB" FT /gene="ML0741" FT /note="Possible pseudogene of M. tuberculosis orthologue FT pstB (Best blastx score 307)" FT /product="ABC transport component of phosphate uptake FT (pseudogene)" FT /pseudo FT CDS 882887..883982 FT /class="III.A.4" FT /colour=0 FT /gene="ML0742" FT /note="Possible pseudogene of M. tuberculosis paralog pstS FT (Best blastx score 440)" FT /product="PstS component of phosphate uptake (pseudogene)" FT /pseudo FT CDS 884241..885771 FT /class="I.J.3" FT /colour=0 FT /gene="pknD" FT /gene="ML0743" FT /note="Possible pseudogene of M. tuberculosis orthologue FT pknD (Best blastx score 458)" FT /product="serine-threonine protein kinase (pseudogene)" FT /pseudo FT CDS complement(885848..886981) FT /class="V" FT /colour=0 FT /gene="ML0744" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3272 (Best blastx score 782)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(887113..887966) FT /class="V" FT /colour=0 FT /gene="ML0745" FT /note="Possible pseudogene similar to M. tuberculosis FT paralogue Rv1729c (Best blastx score 318)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS 888118..888693 FT /class="V" FT /colour=0 FT /gene="ML0746" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3271c (Best blastx score 151)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(888793..890970) FT /class="III.A.2" FT /colour=3 FT /gene="ctpC" FT /gene="ML0747" FT /note="Similar to Mycobacterium tuberculosis probable FT cation-transporting p-type ATPase C ctpC OR RV3270 OR FT MTCY71.10 SW:CTPC_MYCTU (P96875) fasta scores: E(): 0, FT 86.0% id in 713 aa, and to Mycobacterium tuberculosis FT metal transporting atpase mta72 mtaA TR:O66027 FT (EMBL:U82820) fasta scores: E(): 0, 79.4% id in 688 aa. FT Contains hydrophobic, possible membrane-spanning region. FT Contains 2 Pfam matches to entry PF00122 E1-E2_ATPase, FT E1-E2 ATPase. Contains PS00017 ATP/GTP-binding site motif FT A (P-loop). Contains PS00154 E1-E2 ATPases phosphorylation FT site. Contains PS01229 Hypothetical cof family signature FT 2." FT /product="putative cation transport ATPase" FT /tb_orthologue="ctpC" FT misc_feature complement(888823..889374) FT /colour=0 FT /note="Pfam match to entry PF00122 E1-E2_ATPase, E1-E2 FT ATPase, score 216.20, E-value 4.9e-61" FT misc_feature complement(889057..889125) FT /colour=8 FT /note="PS01229 Hypothetical cof family signature 2" FT misc_feature complement(889552..890406) FT /colour=0 FT /note="Pfam match to entry PF00122 E1-E2_ATPase, E1-E2 FT ATPase, score 246.10, E-value 4.7e-70" FT misc_feature complement(889705..889725) FT /colour=8 FT /note="PS00154 E1-E2 ATPases phosphorylation site" FT misc_feature complement(890476..890499) FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT CDS complement(890981..891259) FT /class="VI" FT /colour=10 FT /gene="ML0748" FT /note="Similar to Mycobacterium tuberculosis hypothetical FT 9.8 kDa protein irpA OR RV3269 OR MTCY71.09 TR:P96874 FT (EMBL:Z92771) fasta scores: E(): 5.5e-23, 73.6% id in 91 FT aa, to Mycobacterium tuberculosis hypothetical 9.4 kDa FT protein RV1993C OR MTCY39.26 SW:YJ93_MYCTU (Q10865) fasta FT scores: E(): 6.3e-14, 60.8% id in 79 aa and to FT Mycobacterium tuberculosis hypothetical 10.3 kDa protein FT RV0968 OR MTCY10D7.06C SW:Y968_MYCTU (P71542) fasta FT scores: E(): 4.4e-11, 52.1% id in 96 aa." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv3269" FT CDS complement(891408..892024) FT /class="V" FT /colour=0 FT /gene="ML0749" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3268 (Best blastx score 385)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(892063..893532) FT /class="V" FT /colour=10 FT /gene="ML0750" FT /note="Similar to Mycobacterium tuberculosis hypothetical FT 51.3 kDa protein RV3267 OR MTCY71.07 TR:P96872 FT (EMBL:Z92771) fasta scores: E(): 0, 78.9% id in 498 aa, FT and to Mycobacterium tuberculosis cpsA OR RV3484 OR FT MTCY13E12.37 TR:O06347 (EMBL:Z95390) fasta scores: E(): 0, FT 34.5% id in 502 aa. Also similar to hypothetical proteins FT from capsular polysaccharide biosynthesis loci (homologues FT of Streptococcus agalactiae cpsX, which may have a FT regulatory function) and to frnA from a Streptomyces FT roseofulvus polyketide biosynthesis locus." FT /note="Similar to ML2247" FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv3267" FT CDS 893621..894556 FT /class="I.C.3" FT /colour=7 FT /gene="rmlD" FT /gene="ML0751" FT /note="Similar to Mycobacterium tuberculosis rfbD OR FT RV3266C OR MTCY71.06C TR:P96871 (EMBL:Z92771) fasta FT scores: E(): 0, 74.4% id in 312 aa, to Saccharopolyspora FT erythraea thymidine diphospho-4-keto-6-deoxyglucose 3, FT 5-epimerase kde TR:Q54117 (EMBL:L37354) fasta scores: E(): FT 0, 49.2% id in 301 aa and to Streptomyces griseus FT dTDP-4-dehydrorhamnose reductase strL SW:STRL_STRGR FT (P29781) fasta scores: E(): 1.6e-32, 44.4% id in 311 aa." FT /product="putative dTDP-rhamnose modification protein" FT /tb_orthologue="rmlD" FT CDS 894549..895475 FT /class="I.C.3" FT /colour=7 FT /gene="wbbL" FT /gene="ML0752" FT /note="Similar to Mycobacterium tuberculosis putative FT dTDP-rhamnosyl transferase RV3265C OR MTCY71.05C TR:P96870 FT (EMBL:Z92771) fasta scores: E(): 0, 84.7% id in 301 aa, FT and to Mycobacterium smegmatis FT dTDP-rha:A-D-GlcNAc-diphosphoryl polyprenol, FT A-3-L-rhamnosyl transferase wbbL TR:Q9RN50 (EMBL:AF187550) FT fasta scores: E(): 0, 69.9% id in 296 aa. Contains Pfam FT match to entry PF00535 Glycos_transf_2, Glycosyl FT transferases." FT /product="putative dTDP-rhamnosyl transferase" FT /tb_orthologue="wbbL" FT misc_feature 894564..895076 FT /colour=0 FT /note="Pfam match to entry PF00535 Glycos_transf_2, FT Glycosyl transferases, score 21.90, E-value 0.0029" FT CDS 895477..896553 FT /class="I.C.3" FT /colour=7 FT /gene="rmlA2" FT /gene="ML0753" FT /note="Similar to Mycobacterium tuberculosis hypothetical FT protein RV3264C OR MTCY71.04C TR:P96869 (EMBL:Z92771) FT fasta scores: E(): 0, 86.9% id in 359 aa. Similar to FT Saccharomyces cerevisiae mpg1, mannose-1-phosphate FT guanyltransferase SW:MPG1_YEAST (P41940) blastp score: FT 141.5 bits, 29% identity in 349 aa. Contains Pfam match to FT entry PF00483 NTP_transferase, Nucleotidyl transferase. FT Contains 3 Pfam matches to entry PF00132 hexapep, FT Bacterial transferase hexapeptide (four repeats)." FT /note="ML2503" FT /product="putative sugar-phosphate nucleotidyl FT transferase" FT /tb_orthologue="rmlA2" FT misc_feature 895495..896190 FT /colour=0 FT /note="Pfam match to entry PF00483 NTP_transferase, FT Nucleotidyl transferase, score 227.00, E-value 2.7e-64" FT misc_feature 896281..896334 FT /colour=0 FT /note="Pfam match to entry PF00132 hexapep, Bacterial FT transferase hexapeptide (four repeats), score 16.60, FT E-value 0.59" FT misc_feature 896383..896436 FT /colour=0 FT /note="Pfam match to entry PF00132 hexapep, Bacterial FT transferase hexapeptide (four repeats), score 5.90, FT E-value 1.2e+02" FT misc_feature 896470..896523 FT /colour=0 FT /note="Pfam match to entry PF00132 hexapep, Bacterial FT transferase hexapeptide (four repeats), score 5.10, FT E-value 1.6e+02" FT CDS 896665..897426 FT /class="V" FT /colour=0 FT /gene="ML0754" FT /note="Possible pseudogene similar to M. tuberculosis FT paralogue Rv3714c (Best blastx score 231)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(897493..897753) FT /class="VI" FT /colour=8 FT /gene="ML0755" FT /ml_unique FT /note="Unknown function." FT /product="hypothetical protein" FT CDS complement(897971..899638) FT /class="II.A.5" FT /colour=2 FT /gene="ML0756" FT /note="Similar to Mycobacterium tuberculosis putative DNA FT methylase RV3263 OR MTCY71.03 TR:P96868 (EMBL:Z92771) FT fasta scores: E(): 0, 81.9% id in 541 aa and to FT Escherichia coli modification methylase eco57IM FT SW:MT57_ECOLI (P25240) fasta scores: E(): 0, 31.4% id in FT 525 aa. Contains PS00092 N-6 Adenine-specific DNA FT methylases signature." FT /product="probable DNA methylase" FT /tb_orthologue="Rv3263" FT misc_feature complement(899279..899299) FT /colour=8 FT /note="PS00092 N-6 Adenine-specific DNA methylases FT signature" FT CDS complement(899660..900352) FT /class="VI" FT /colour=8 FT /gene="ML0757" FT /ml_unique FT /note="Unknown function." FT /product="hypothetical protein" FT CDS complement(900393..901766) FT /class="I.B.7" FT /colour=7 FT /gene="ML0758" FT /note="Similar to Mycobacterium tuberculosis conserved FT hypothetical protein RV3262 OR MTCY71.02 TR:P96867 FT (EMBL:Z92771) fasta scores: E(): 0, 82.2% id in 445 aa, FT and to Streptomyces coelicolor putative oxidoreductase FT SCE34.18 TR:CAB88921 (EMBL:AL353862) fasta scores: E(): 0, FT 50.8% id in 455 aa. Contains Pfam match to entry PF00881 FT Nitroreductase, Nitroreductase family. Contains Pfam match FT to entry PF01996 DUF129, Protein of unknown function." FT /product="putative oxidoreductase" FT /tb_orthologue="Rv3262" FT misc_feature complement(900501..900986) FT /colour=0 FT /note="Pfam match to entry PF00881 Nitroreductase, FT Nitroreductase family, score 23.70, E-value 6.3e-05" FT misc_feature complement(901011..901706) FT /colour=0 FT /note="Pfam match to entry PF01996 DUF129, Protein of FT unknown function, score 329.50, E-value 3.9e-95" FT CDS complement(901763..902902) FT /class="V" FT /colour=10 FT /gene="ML0759" FT /note="Similar to Mycobacterium tuberculosis hypothetical FT 35.3 kDa protein RV3261 OR MTCY71.01 TR:P96866 FT (EMBL:Z92771) fasta scores: E(): 0, 79.0% id in 333 aa and FT to Streptomyces coelicolor hypothetical 33.6 kDa protein FT SCE34.17 TR:CAB88920 (EMBL:AL353862) fasta scores: E(): 0, FT 52.7% id in 334 aa. Contains Pfam match to entry PF01933 FT UPF0052, Uncharacterised protein family." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv3261" FT misc_feature complement(901769..902758) FT /colour=0 FT /note="Pfam match to entry PF01933 UPF0052, FT Uncharacterised protein family, score 638.90, E-value FT 2.8e-188" FT RBS 903119..903123 FT /note="possible RBS" FT CDS 903134..903496 FT /class="I.J.1" FT /colour=9 FT /gene="whiB2" FT /gene="whmD" FT /gene="ML0760" FT /note="Similar to Mycobacterium tuberculosis regulatory FT protein whiB2 OR RV3260C OR MTV015.05C TR:O53353 FT (EMBL:AL021840) fasta scores: E(): 0, 85.4% id in 89 aa, FT to Mycobacterium smegmatis whmD TR:Q9S426 (EMBL:AF164439) FT fasta scores: E(): 1.6e-29, 84.1% id in 82 aa and to FT Streptomyces coelicolor regulatory protein WHIB TR:Q53963 FT (EMBL:X62287) fasta scores: E(): 4.8e-26, 85.5% id in 69 FT aa." FT /note="Similar to ML0382, ML0639, ML0804 and ML2307" FT /product="putative transcriptional regulator" FT /tb_orthologue="whiB2" FT CDS complement(903525..904028) FT /class="V" FT /colour=10 FT /gene="ML0761" FT /note="Similar to Mycobacterium tuberculosis hypothetical FT 15.6 kDa protein RV3259 OR MTV015.04 TR:O53352 FT (EMBL:AL021840) fasta scores: E(): 0, 89.2% id in 139 aa FT and to Mycobacterium smegmatis hypothetical 6.0 kDa FT protein (partial CDS) TR:Q9S425 (EMBL:AF164439) fasta FT scores: E(): 1e-10, 75.5% id in 53 aa." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv3259" FT CDS 904068..904565 FT /class="V" FT /colour=10 FT /gene="ML0762" FT /note="Similar to Mycobacterium tuberculosis hypothetical FT 16.8 kDa protein RV3258C OR MTV015.03C TR:O53351 FT (EMBL:AL021840) fasta scores: E(): 0, 76.9% id in 169 aa, FT and to Streptomyces coelicolor hypothetical 15.0 kDa FT protein SCE34.11C TR:CAB88914 (EMBL:AL353862) fasta FT scores: E(): 4.8e-16, 47.0% id in 151 aa." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv3258c" FT RBS 904664..904669 FT /note="possible RBS" FT CDS 904675..906081 FT /class="I.A.1" FT /colour=7 FT /gene="pmmA" FT /gene="ML0763" FT /note="Similar to Mycobacterium tuberculosis pmmA, FT RV3257C, putative phosphomannomutase, TR:O86374 FT (EMBL:Z95121) fasta scores: E(): 0, 83.1% id in 468 aa and FT to Escherichia coli phosphomannomutase manB SW:MANB_ECOLI FT (P24175) fasta scores: E(): 0, 39.0% id in 451 aa. FT Contains Pfam match to entry PF00408 PGM_PMM, FT Phosphoglucomutase/phosphomannomutase." FT /note="Similar to ML0366 and ML0706" FT /product="putative phosphomannomutase" FT /tb_orthologue="pmmA" FT misc_feature 904696..906015 FT /colour=0 FT /note="Pfam match to entry PF00408 PGM_PMM, FT Phosphoglucomutase/phosphomannomutase, score 385.40, FT E-value 5.5e-112" FT RBS 906066..906070 FT /note="possible RBS" FT CDS 906078..907175 FT /class="V" FT /colour=10 FT /gene="ML0764" FT /note="Similar to Mycobacterium tuberculosis hypothetical FT 35.3 kDa protein RV3256C OR MTV015.01C-MTCY20B11.31C FT TR:O05899 (EMBL:Z95121) fasta scores: E(): 0, 75.3% id in FT 365 aa." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv3256c" FT CDS 907183..908415 FT /class="I.A.1" FT /colour=7 FT /gene="manA" FT /gene="ML0765" FT /note="Similar to Mycobacterium tuberculosis manA OR FT RV3255C OR MTCY20B11.30C, putative mannose-6-phosphate FT isomerase, TR:O05898 (EMBL:Z95121) fasta scores: E(): 0, FT 84.4% id in 411 aa and to Homo sapiens mannose-6-phosphate FT isomerase mpi SW:MANA_HUMAN (P34949) fasta scores: E(): FT 1.2e-27, 31.0% id in 432 aa. Contains Pfam match to entry FT PF01238 PMI_typeI, Phosphomannose isomerase type I." FT /product="putative mannose-6-phosphate isomerase" FT /tb_orthologue="manA" FT misc_feature 907189..908304 FT /colour=0 FT /note="Pfam match to entry PF01238 PMI_typeI, FT Phosphomannose isomerase type I, score 209.40, E-value FT 5.5e-59" FT CDS complement(908396..909753) FT /class="I.B.7" FT /colour=0 FT /gene="ML0766" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3254 (Best blastx score 402)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS 909846..911322 FT /class="III.A.1" FT /colour=0 FT /gene="ML0767" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3253c (Best blastx score 1109)" FT /product="probable cationic amino acid transporter FT (pseudogene)" FT /pseudo FT CDS 911577..912770 FT /class="I.B.7" FT /colour=0 FT /gene="ML0768" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3252c (Best blastx score 358)" FT /product="possible alkane-1 monooxygenase (pseudogene)" FT /pseudo FT CDS 912779..912895 FT /class="I.B.6.c" FT /colour=0 FT /gene="ML0769" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3251c (Best blastx score 105)" FT /product="possible rubredoxin (pseudogene)" FT /pseudo FT CDS 912998..913508 FT /class="I.J.1" FT /colour=0 FT /gene="ML0770" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3249c (Best blastx score 273)" FT /product="transcriptional regulator (TetR/AcrR family) FT (pseudogene)" FT /pseudo FT RBS 913613..913616 FT /note="possible RBS" FT CDS 913623..915101 FT /class="I.C.5" FT /colour=7 FT /gene="sahH" FT /gene="ML0771" FT /note="Similar to Mycobacterium tuberculosis FT adenosylhomocysteinase achY OR sahH OR RV3248C OR FT MTCY20B11.23C SW:SAHH_MYCTU (O08364) fasta scores: E(): 0, FT 91.4% id in 489 aa, and to Mycobacterium bovis FT S-adenosyl-L-homocysteine hydrolase TR:AAF72670 FT (EMBL:AF262755) fasta scores: E(): 0, 91.4% id in 489 aa, FT and to Trichomonas vaginalis adenosylhomocysteinase FT SW:SAHH_TRIVA (P51540) fasta scores: E(): 0, 62.3% id in FT 478 aa. Contains Pfam match to entry PF00670 AdoHcyase, FT S-adenosyl-L-homocysteine hydrolase. Contains PS00739 FT S-adenosyl-L-homocysteine hydrolase signature 2. Contains FT PS00738 S-adenosyl-L-homocysteine hydrolase signature 1." FT /product="putative S-adenosyl-L-homocysteine hydrolase" FT /tb_orthologue="sahH" FT misc_feature 913677..914927 FT /colour=0 FT /note="Pfam match to entry PF00670 AdoHcyase, FT S-adenosyl-L-homocysteine hydrolase, score 943.80, E-value FT 4.6e-280" FT misc_feature 913887..913931 FT /colour=8 FT /note="PS00738 S-adenosyl-L-homocysteine hydrolase FT signature 1" FT misc_feature 914436..914486 FT /colour=8 FT /note="PS00739 S-adenosyl-L-homocysteine hydrolase FT signature 2" FT CDS 915144..915776 FT /class="I.F.3" FT /colour=7 FT /gene="tmk" FT /gene="ML0772" FT /note="Similar to Mycobacterium tuberculosis putative FT thymidylate kinase MTCY20B11 OR RV3247C OR MTCY20B11.22C FT TR:O05891 (EMBL:Z95121) fasta scores: E(): 0, 77.3% id in FT 207 aa, and to Homo sapiens thymidylate kinase tymK OR FT tmpK SW:KTHY_HUMAN (P23919) fasta scores: E(): 0.005, FT 29.1% id in 196 aa. Contains Pfam match to entry PF02223 FT Thymidylate_kin, Thymidylate kinase. Contains PS01331 FT Thymidylate kinase signature. Contains PS00017 FT ATP/GTP-binding site motif A (P-loop). Contains PS00013 FT Prokaryotic membrane lipoprotein lipid attachment site." FT /product="putative thymidylate kinase" FT /tb_orthologue="tmk" FT misc_feature 915156..915764 FT /colour=0 FT /note="Pfam match to entry PF02223 Thymidylate_kin, FT Thymidylate kinase, score 160.70, E-value 2.5e-44" FT misc_feature 915210..915233 FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT misc_feature 915417..915455 FT /colour=8 FT /note="PS01331 Thymidylate kinase signature" FT misc_feature 915741..915773 FT /colour=8 FT /note="PS00013 Prokaryotic membrane lipoprotein lipid FT attachment site" FT CDS 915869..916555 FT /class="I.J.2" FT /colour=9 FT /gene="mtrA" FT /gene="ML0773" FT /note="Similar to Mycobacterium tuberculosis mtra protein FT mtrA OR RV3246C OR MTCY20B11.21C TR:Q50447 (EMBL:Z95121) FT fasta scores: E(): 0, 98.7% id in 228 aa, to Mycobacterium FT bovis sensory transduction protein regX3 SW:RGX3_MYCBO FT (O07130) fasta scores: E(): 0, 50.2% id in 221 aa, and to FT Streptomyces coelicolor transcriptional regulatory protein FT afsQ1 SW:AFQ1_STRCO (Q04942) fasta scores: E(): 0, 47.1% FT id in 221 aa. Contains Pfam match to entry PF00486 FT trans_reg_C, Transcriptional regulatory protein, C FT terminal. Contains Pfam match to entry PF00072 FT response_reg, Response regulator receiver domain." FT /note="Similar to ML0174, ML1286, ML2123 and ML2439" FT /product="putative two-component response regulator" FT /tb_orthologue="mtrA" FT misc_feature 915884..916216 FT /colour=0 FT /note="Pfam match to entry PF00072 response_reg, Response FT regulator receiver domain, score 129.60, E-value 5.8e-35" FT misc_feature 916316..916534 FT /colour=0 FT /note="Pfam match to entry PF00486 trans_reg_C, FT Transcriptional regulatory protein, C terminal, score FT 111.50, E-value 4.7e-32" FT CDS 916683..918371 FT /class="I.J.2" FT /colour=9 FT /gene="mtrB" FT /gene="ML0774" FT /note="Similar to Mycobacterium tuberculosis mtrB, FT probable sensor histidine kinase, TR:Q50496 (EMBL:U14909) FT fasta scores: E(): 0, 88.3% id in 566 aa. Contains FT hydrophobic, possible membrane-spanning region. Contains FT Pfam match to entry PF00672 DUF5, Domain found in FT bacterial signal proteins. Contains Pfam match to entry FT PF00512 signal, Histidine kinase." FT /note="Similar to ML0175, ML2124 and ML2440" FT /product="putative two-component system sensor kinase" FT /tb_orthologue="mtrB" FT misc_feature 917325..917534 FT /colour=0 FT /note="Pfam match to entry PF00672 DUF5, Domain found in FT bacterial signal proteins, score 69.10, E-value 9.1e-17" FT misc_feature 917595..918230 FT /colour=0 FT /note="Pfam match to entry PF00512 signal, Histidine FT kinase, score 189.50, E-value 5.2e-53" FT RBS 918360..918364 FT /note="possible RBS" FT CDS 918368..920137 FT /class="II.C.1" FT /colour=3 FT /gene="lpqB" FT /gene="ML0775" FT /note="Similar to Mycobacterium tuberculosis putative FT lipoprotein RV3244C OR MTCY20B11.19C TR:O05889 FT (EMBL:Z95121) fasta scores: E(): 0, 87.9% id in 579 aa, FT and to Streptomyces coelicolor putative lipoprotein FT SCE33.13C TR:CAB90922 (EMBL:AL355774) fasta scores: E(): FT 0.00039, 24.4% id in 624 aa. Contains a probable FT N-terminal signal sequence. Contains PS00013 Prokaryotic FT membrane lipoprotein lipid attachment site." FT /product="putative lipoprotein" FT /tb_orthologue="lpqB" FT misc_feature 918398..918430 FT /colour=8 FT /note="PS00013 Prokaryotic membrane lipoprotein lipid FT attachment site" FT CDS 920258..920515 FT /class="V" FT /colour=10 FT /gene="ML0776" FT /note="Similar to the N-terminal half of Mycobacterium FT tuberculosis hypothetical 22.5 kDa protein RV3242C OR FT MTCY20B11.17C TR:O05887 (EMBL:Z95121) fasta scores: E(): FT 6.8e-18, 78.1% id in 64 aa." FT CDS 920655..920849 FT /class="VI" FT /colour=8 FT /gene="ML0777" FT /ml_unique FT /note="Unknown function." FT /product="hypothetical protein" FT CDS 920953..921642 FT /class="V" FT /colour=10 FT /gene="ML0778" FT /note="Similar to Mycobacterium tuberculosis hypothetical FT 24.5 kDa protein RV3241C OR MTCY20B11.16C TR:O05886 FT (EMBL:Z95121) fasta scores: E(): 0, 89.3% id in 206 aa and FT to Spinacia oleracea 30s ribosomal protein S30, FT chloroplast precursor RPS30 OR RPS22 SW:RR30_SPIOL FT (P19954) fasta scores: E(): 1.3e-10, 26.8% id in 213 aa." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv3241c" FT CDS 921722..924544 FT /class="III.D" FT /colour=3 FT /gene="secA" FT /gene="ML0779" FT /note="Similar to Mycobacterium tuberculosis preprotein FT translocase secA 1 subunit OR SECA OR RV3240C OR FT MTCY20B11.15C SW:SEA1_MYCTU (O05885) fasta scores: E(): 0, FT 87.5% id in 949 aa and to Mycobacterium bovis preprotein FT translocase secA subunit SECA SW:SECA_MYCBO (P71494) fasta FT scores: E(): 0, 85.9% id in 949 aa. Contains Pfam match to FT entry PF01043 SecA_protein, SecA protein, amino terminal FT region. Contains PS01312 Protein secA signatures." FT /note="Similar to ML2082" FT /product="putative preprotein translocase subunit" FT /tb_orthologue="secA" FT misc_feature 921725..922978 FT /colour=0 FT /note="Pfam match to entry PF01043 SecA_protein, SecA FT protein, amino terminal region, score 1050.40, E-value 0" FT misc_feature 923162..923209 FT /colour=8 FT /note="PS01312 Protein secA signatures" FT CDS 924814..925329 FT /class="II.C.4" FT /colour=0 FT /gene="ML0780" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3238c (Best blastx score 169)" FT /product="putative membrane protein (pseudogene)" FT /pseudo FT CDS 925681..926163 FT /class="V" FT /colour=10 FT /gene="ML0781" FT /note="Similar to Mycobacterium tuberculosis hypothetical FT 17.2 kDa protein RV3237C OR MTCY20B11.12C TR:O05882 FT (EMBL:Z95121) fasta scores: E(): 0, 80.6% id in 160 aa, to FT Deinococcus radiodurans conserved hypothetical protein FT DR1148 TR:Q9RV81 (EMBL:AE001964) fasta scores: E(): FT 1.1e-19, 38.0% id in 158 aa and to Streptomyces coelicolor FT hypothetical 17.1 kDa protein SC66T3.12 TR:Q9XAK1 FT (EMBL:AL079348) fasta scores: E(): 5.5e-14, 37.9% id in FT 153 aa." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv3237c" FT CDS 926168..927325 FT /class="III.A.2" FT /colour=3 FT /gene="ML0782" FT /note="Similar to Mycobacterium tuberculosis putative FT transmembrane transport protein RV3236C OR MTCY20B11.11C FT TR:O05881 (EMBL:Z95121) fasta scores: E(): 0, 81.8% id in FT 385 aa, and to Escherichia coli glutathione-regulated FT potassium-efflux system protein kefB OR trkB SW:KEFB_ECOLI FT (P45522) fasta scores: E(): 4.7e-13, 26.0% id in 362 aa. FT Contains hydrophobic, possible membrane-spanning regions." FT /product="putative transmembrane transport protein" FT /tb_orthologue="kefB" FT CDS complement(927401..927982) FT /class="V" FT /colour=0 FT /gene="ML0783" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3235 (Best blastx score 155)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS 928181..928874 FT /class="V" FT /colour=0 FT /gene="ML0784" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3234c (Best blastx score 286)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS 928967..929496 FT /class="V" FT /colour=0 FT /gene="ML0785" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3233c (Best blastx score 202)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS 929727..930223 FT /class="II.A.7" FT /colour=0 FT /gene="ML0786" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3232c (Best blastx score 85)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS 930316..930630 FT /class="V" FT /colour=0 FT /gene="ML0787" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3231c (Best blastx score 184)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS 930894..931942 FT /class="I.B.7" FT /colour=0 FT /gene="ML0788" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3230c (Best blastx score 278)" FT /product="putative oxidoreductase (pseudogene)" FT /pseudo FT CDS 932051..933307 FT /class="I.H.2" FT /colour=0 FT /gene="desA3" FT /gene="ML0789" FT /note="Possible pseudogene of M. tuberculosis orthologue FT desA3 (Best blastx score 343)" FT /product="acyl-[ACP] desaturase (pseudogene)" FT /pseudo FT CDS complement(933510..934326) FT /class="I.A.3" FT /colour=0 FT /gene="ML0790" FT /note="Possible pseudogene similar to M. tuberculosis FT putative beta-ketoadipyl CoA thiolases (Best blastx score FT 143)" FT /product="beta-ketoadipyl-CoA thiolase (pseudogene)" FT /pseudo FT CDS complement(934528..935511) FT /class="V" FT /colour=10 FT /gene="ML0791" FT /note="Similar to Mycobacterium tuberculosis hypothetical FT 34.9 kDa protein RV3228 OR MTCY20B11.03 TR:O05873 FT (EMBL:Z95121) fasta scores: E(): 0, 84.0% id in 331 aa. FT Contains PS00017 ATP/GTP-binding site motif A (P-loop)." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv3228" FT misc_feature complement(934876..934899) FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT CDS complement(935508..936800) FT /EC_number="2.5.1.19" FT /class="I.D.4" FT /colour=7 FT /gene="aroA" FT /gene="ML0792" FT /note="Similar to Mycobacterium tuberculosis FT 3-phosphoshikimate 1-carboxyvinyltransferase aroA OR FT RV3227 OR MTCY20B11.02 SW:AROA_MYCTU (P22487) fasta FT scores: E(): 0, 79.0% id in 428 aa, and to Yersinia FT enterocolitica 3-phosphoshikimate FT 1-carboxyvinyltransferase aroA SW:AROA_YEREN (P19688) FT fasta scores: E(): 1.2e-31, 33.1% id in 432 aa. Contains FT Pfam match to entry PF00275 EPSP_syntase, EPSP synthase FT (3-phosphoshikimate 1-carboxyvinyltransferase). Contains FT PS00885 EPSP synthase signature 2." FT /product="putative 3-phosphoshikimate 1-carboxyvinyl FT transferase" FT /tb_orthologue="aroA" FT RBS complement(935519..935525) FT /note="possible RBS" FT misc_feature complement(935538..936776) FT /colour=0 FT /note="Pfam match to entry PF00275 EPSP_syntase, EPSP FT synthase (3-phosphoshikimate 1-carboxyvinyltransferase), FT score 608.10, E-value 5.3e-179" FT misc_feature complement(935727..935783) FT /colour=8 FT /note="PS00885 EPSP synthase signature 2" FT RBS complement(936806..936812) FT /note="possible RBS" FT CDS 936847..937605 FT /class="IV.B.3" FT /colour=12 FT /gene="ML0793" FT /note="Similar to Mycobacterium tuberculosis hypothetical FT 27.6 kDa protein RV3226C OR MTCY20B11.01C TR:O05872 FT (EMBL:Z95121) fasta scores: E(): 0, 70.6% id in 252 aa, FT and to Bacteriophage SPBc2 yoqW protein TR:O64131 FT (EMBL:AF020713) fasta scores: E(): 4.8e-21, 36.5% id in FT 244 aa." FT /product="putative bacteriophage protein" FT /tb_orthologue="Rv3226c" FT CDS complement(937636..938913) FT /class="IV.B.2" FT /colour=0 FT /gene="ML0794" FT /note="Possible pseudogene of M. tuberculosis REP-family FT proteins (Best blastx score 135)" FT /product="REP-family protein (pseudogene)" FT /pseudo FT CDS complement(939222..941360) FT /class="I.C.5" FT /colour=0 FT /gene="atsD" FT /gene="ML0795" FT /note="Possible pseudogene of M. tuberculosis orthologue FT atsD (Best blastx score 485)" FT /product="putative arylsulfatase (pseudogene)" FT /pseudo FT CDS 942266..942442 FT /class="V" FT /colour=10 FT /gene="ML0796" FT /note="Similar to the N-termini of Mycobacterium FT tuberculosis putative response regulator RV0844C OR FT MTV043.37C TR:O53856 (EMBL:AL022004) fasta scores: E(): FT 0.2, 39.7% id in 63 aa and Streptomyces coelicolor absA2 FT OR SCE8.19 TR:Q53894 (EMBL:U51332) fasta scores: E(): 4.3, FT 38.5% id in 52 aa. May be a on ORF within a regulator FT protein pseudogene." FT /product="hypothetical protein" FT CDS complement(943529..944968) FT /class="IV.C.2" FT /colour=0 FT /gene="PPE" FT /gene="ML0797" FT /note="Possible pseudogene similar to M. tuberculosis FT PPE-family proteins (Best blastx score 193)" FT /product="PPE-family protein (pseudogene)" FT /pseudo FT CDS complement(946524..948302) FT /class="V" FT /colour=10 FT /gene="ML0798" FT /note="Similar to Mycobacterium tuberculosis ATP/GTP FT binding protein Rv2787 or MTV002.52 TR:O33329 FT (EMBL:AL008967) fasta scores: E(): 0, 76.5% id in 587 aa FT and to other M. tuberculosis hypothetical proteins. FT Contains PS00017 ATP/GTP-binding site motif A (P-loop)." FT /note="C-terminal half is similar to that of ML0048. Also FT similar to ML1536" FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv2787" FT misc_feature complement(947298..947321) FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT RBS complement(948312..948316) FT /note="possible RBS" FT CDS complement(949041..949448) FT /class="V" FT /colour=10 FT /gene="ML0799" FT /ml_unique FT /note="Similar to Mycobacterium smegmatis hypothetical FT 19.1 kDa protein TR:Q9XCD9 (EMBL:AF144091) fasta scores: FT E(): 1e-15, 60.2% id in 93 aa and to other hypothetical FT proteins e.g. Salmonella typhimurium 17.0 kDa protein in FT ushA 3'region ybaK SW:YBAK_SALTY (P37174) fasta scores: FT E(): 6e-08, 40.9% id in 88 aa." FT /product="conserved hypothetical protein" FT CDS complement(949526..950328) FT /class="I.B.7" FT /colour=0 FT /gene="ML0800" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3224 (Best blastx score 426)" FT /product="putative oxidoreductase (pseudogene)" FT /pseudo FT CDS 950848..951067 FT /class="II.A.7" FT /colour=0 FT /gene="sigH" FT /gene="ML0801" FT /note="Possible pseudogene of M. tuberculosis orthologue FT sigH (Best blastx score 162)" FT /product="ECF subfamily sigma subunit (pseudogene)" FT /pseudo FT CDS 951814..952053 FT /class="V" FT /colour=10 FT /gene="ML0802" FT /note="Similar to Mycobacterium tuberculosis hypothetical FT 7.3 kDa protein Rv3221c or MTCY07d11.05 TR:O05845 FT (EMBL:Z95120) fasta scores: E(): 6.2e-19, 90.1% id in 71 FT aa and to the C-terminal half of Propionigenium modestum FT methylmalonyl-CoA decarboxylase, gamma-subunit mmdC (134 FT aa) TR:O54030 (EMBL:AJ002015) fasta scores: E(): 3.4e-06, FT 42.9% id in 70 aa. Contains Pfam match to entry PF00364 FT biotin_lipoyl, Biotin-requiring enzymes. Contains PS00445 FT FGGY family of carbohydrate kinases signature 2." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv3221c" FT misc_feature 951817..951882 FT /colour=8 FT /note="PS00445 FGGY family of carbohydrate kinases FT signature 2" FT misc_feature 951844..952047 FT /colour=0 FT /note="Pfam match to entry PF00364 biotin_lipoyl, FT Biotin-requiring enzymes, score 59.20, E-value 9.2e-14" FT RBS 952093..952098 FT /note="possible RBS" FT CDS 952103..953605 FT /class="I.J.2" FT /colour=9 FT /gene="ML0803" FT /note="Similar to Mycobacterium tuberculosis hypothetical FT 54.0 kDa protein Rv3220c or MTCY07D11.06 TR:O05846 FT (EMBL:Z95120) fasta scores: E(): 0, 81.8% id in 501 aa and FT shows weak similarity to Pseudomonas aeruginosa alginate FT biosynthesis sensor protein kinB TR:O34206 (EMBL:U97063) FT fasta scores: E(): 1.3e-06, 26.9% id in 346 aa. Contains FT Pfam match to entry PF00512 signal, Histidine kinase." FT /product="putative two-component system sensor kinase" FT /tb_orthologue="Rv3220c" FT misc_feature 953006..953575 FT /colour=0 FT /note="Pfam match to entry PF00512 signal, Histidine FT kinase, score -32.30, E-value 0.0027" FT CDS complement(953665..953919) FT /class="I.J.1" FT /colour=9 FT /gene="whiB1" FT /gene="whmE" FT /gene="ML0804" FT /note="Similar to Mycobacterium tuberculosis hypothetical FT 9.3 kDa protein Rv3219 or MTCY07D11.07c TR:O05847 FT (EMBL:Z95120) fasta scores: E(): 0, 95.2% id in 84 aa, to FT Mycobacterium smegmatis whiB3 TR:Q9Z6E9 (EMBL:AF073300) FT fasta scores: E(): 2e-10, 43.2% id in 81 aa and to FT Streptomyces coelicolor WhiD protein whiD or SC6G4.45c or FT wblB TR:O88103 (EMBL:AJ010601) fasta scores: E(): 2.7e-10, FT 50.7% id in 75 aa." FT /note="Similar to ML0382, ML0639, ML0760 and ML2307" FT /product="putative transcriptional regulator" FT /tb_orthologue="whiB1" FT RBS complement(953928..953934) FT /note="possible RBS" FT CDS complement(954248..955184) FT /class="V" FT /colour=0 FT /gene="ML0805" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3218 (Best blastx score 324)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS 955206..955727 FT /class="II.C.4" FT /colour=3 FT /gene="ML0806" FT /note="Similar to Mycobacterium tuberculosis hypothetical FT 14.3 kDa protein Rv3217c or MTCY07D11.09 TR:O05849 FT (EMBL:Z95120) fasta scores: E(): 0, 77.1% id in 140 aa. FT Contains hydrophobic, possible membrane-spanning regions." FT /product="putative membrane protein" FT /tb_orthologue="Rv3217c" FT CDS complement(955682..955997) FT /class="IV.H" FT /colour=0 FT /gene="ML0807" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3216 (Best blastx score 171)" FT /product="possible acyltransferase (pseudogene)" FT /pseudo FT CDS complement(956155..957888) FT /class="I.G.14" FT /colour=7 FT /gene="entC" FT /gene="ML0808" FT /note="Similar to Mycobacterium tuberculosis putative FT isochorismate synthase entC or Rv3215 or MTCY07D11.11c FT (372 aa) TR:O05851 (EMBL:Z95120) fasta scores: E(): 0, FT 73.5% id in 366 aa and to Bacillus subtilis isochorismate FT synthase dhbC (398 aa) SW:DHBC_BACSU (P45744) fasta FT scores: E(): 0, 37.9% id in 396 aa. The predicted CDS has FT an N-terminal extension of approx. 210 aa not present in FT known isochorismate synthases. Contains Pfam match to FT entry PF00425 chorismate_bind, chorismate binding enzyme." FT /note="Similar to ML0236 and ML1269" FT /product="putative isochorismate synthase" FT /tb_orthologue="entC" FT misc_feature complement(956170..956991) FT /colour=0 FT /note="Pfam match to entry PF00425 chorismate_bind, FT chorismate binding enzyme, score 237.20, E-value 6.5e-68" FT CDS 958210..958641 FT /class="V" FT /colour=0 FT /gene="ML0809" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3213c (Best blastx score 239)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(958882..960105) FT /class="II.C.4" FT /colour=3 FT /gene="ML0810" FT /note="Similar to Mycobacterium tuberculosis hypothetical FT 42.5 kDa protein Rv3212 or MTCY07D11.14c (407 aa) FT TR:O05854 (EMBL:Z95120) fasta scores: E(): 0, 79.9% id in FT 407 aa. Contains hydrophobic, possible membrane-spanning FT region." FT /product="putative membrane protein" FT /tb_orthologue="Rv3212" FT CDS complement(960117..961751) FT /class="II.A.7" FT /colour=2 FT /gene="rhlE" FT /gene="ML0811" FT /note="Similar to Mycobacterium tuberculosis putative FT ATP-dependent RNA helicase Rv3211 or MTCY07D11.15c (407 FT aa) TR:O05855 (EMBL:Z95120) fasta scores: E(): 0, 74.8% id FT in 531 aa, and to Klebsiella pneumoniae ATP-dependent RNA FT helicase deaD SW:DEAD_KLEPN (P33906) fasta scores: E(): 0, FT 43.2% id in 380 aa. Contains Pfam match to entry PF00271 FT helicase_C, Helicases conserved C-terminal domain. FT Contains Pfam match to entry PF00270 DEAD, DEAD/DEAH box FT helicase. Contains PS00017 ATP/GTP-binding site motif A FT (P-loop). Contains PS00039 DEAD-box subfamily FT ATP-dependent helicases signature." FT /product="putative ATP-dependent RNA helicase" FT /tb_orthologue="rhlE" FT misc_feature complement(960612..960857) FT /colour=0 FT /note="Pfam match to entry PF00271 helicase_C, Helicases FT conserved C-terminal domain, score 119.80, E-value FT 5.3e-32" FT misc_feature complement(960966..961619) FT /colour=0 FT /note="Pfam match to entry PF00270 DEAD, DEAD/DEAH box FT helicase, score 200.20, E-value 2.2e-62" FT misc_feature complement(961152..961178) FT /colour=8 FT /note="PS00039 DEAD-box subfamily ATP-dependent helicases FT signature" FT misc_feature complement(961503..961526) FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT CDS 961966..962652 FT /class="V" FT /colour=0 FT /gene="ML0812" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3210c (Best blastx score 523)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(962707..963294) FT /class="II.C.4" FT /colour=3 FT /gene="ML0813" FT /note="Similar to Mycobacterium tuberculosis hypothetical FT 19.1 kDa protein Rv3209 or MTCY07D11.17c (186 aa) FT TR:O05857 (EMBL:Z95120) fasta scores: E(): 8.5e-23, 58.4% FT id in 185 aa and to Mycobacterium tuberculosis putative FT membrane protein mmpS3 or Rv2198c or MTCY190.09c (299 aa) FT SW:MMS3_MYCTU (Q10390) fasta scores: E(): 9.4e-08, 31.0% FT id in 203 aa. Contains hydrophobic, possible FT membrane-spanning region." FT /note="Similar to ML0431 and ML0877" FT /product="putative membrane protein" FT CDS complement(963428..963751) FT /class="VI" FT /colour=8 FT /gene="ML0814" FT /ml_unique FT /note="Unknown function." FT /product="hypothetical protein" FT CDS complement(963828..964514) FT /class="I.J.1" FT /colour=9 FT /gene="ML0815" FT /note="Similar to Mycobacterium tuberculosis putative FT TetR-family transcriptional regulator Rv3208 or FT MTCY07D11.18c (228 aa) TR:O05858 (EMBL:Z95120) fasta FT scores: E(): 0, 82.4% id in 227 aa. Contains a probable FT helix-turn-helix motif at aa 60-81 (Score 1517, SD +4.35) FT Contains Pfam match to entry PF00440 tetR, Bacterial FT regulatory proteins, tetR family." FT /product="putative TetR-family transcriptional regulator" FT /tb_orthologue="Rv3208" FT misc_feature complement(964245..964385) FT /colour=0 FT /note="Pfam match to entry PF00440 tetR, Bacterial FT regulatory proteins, tetR family, score 71.30, E-value FT 2.1e-17" FT RBS complement(964519..964523) FT /note="possible RBS" FT CDS 964701..965726 FT /class="V" FT /colour=10 FT /gene="ML0816" FT /note="Similar to Mycobacterium tuberculosis hypothetical FT 31.0 kDa protein Rv3207c or MTCY07D11.19 (285 aa) FT TR:O05859 (EMBL:Z95120) fasta scores: E(): 0, 80.6% id in FT 284 aa, and to Streptomyces coelicolor putative membrane FT protein SC2H12.28c (314 aa) TR:CAB94652 (EMBL:AL359215) FT fasta scores: E(): 1e-13, 30.2% id in 331 aa." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv3207c" FT RBS 965916..965920 FT /note="possible RBS" FT CDS 965930..967117 FT /class="I.G.4" FT /colour=7 FT /gene="moeZ" FT /gene="ML0817" FT /note="Similar to M. tuberculosis moeZ, Rv3206c, moeB FT homologue, TR:O05860 (EMBL:AL123456) (392 aa); Fasta score FT E(): 0, 86.4% identity in 391 aa overlap and to M. FT tuberculosis moeB, Rv3116, putative molybdopterin FT biosynthesis protein, TR:O05792 (EMBL:AL123456) (389 aa); FT Fasta score E(): 0, 57.8% identity in 384 aa overlap. FT Similar to many e.g. Arabidopsis thaliana cnx5, FT molybdopterin synthase sulphurylase, TR:Q9ZNW0 FT (EMBL:AF124160) (464 aa); Fasta score E(): 0, 34.5% FT identity in 406 aa overlap. Contains Pfam match to entry FT PF00581 Rhodanese, Rhodanese-like domain. Contains Pfam FT match to entry PF00899 ThiF_family, ThiF family." FT /product="probably involved in molybdopterin biosynthesis" FT /tb_orthologue="moeZ" FT misc_feature 966059..966469 FT /colour=0 FT /note="Pfam match to entry PF00899 ThiF_family, ThiF FT family, score 220.20, E-value 3e-62" FT misc_feature 966806..967090 FT /colour=0 FT /note="Pfam match to entry PF00581 Rhodanese, FT Rhodanese-like domain, score 49.80, E-value 6.2e-11" FT CDS 967172..968065 FT /class="V" FT /colour=10 FT /gene="ML0818" FT /note="Similar to M. tuberculosis Rv3205c, hypothetical FT protein, TR:O05861 (EMBL:AL123456) (292 aa); Fasta score FT E(): 0, 87.3% identity in 291 aa overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv3205c" FT CDS complement(968156..968444) FT /class="IV.H" FT /colour=0 FT /gene="ML0819" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3204 (Best blastx score 245)" FT /product="putative methyltransferase (pseudogene)" FT /pseudo FT CDS complement(968478..969131) FT /class="II.B.5" FT /colour=0 FT /gene="lipV" FT /gene="ML0820" FT /note="Possible pseudogene of M. tuberculosis orthologue FT lipV (Best blastx score 236)" FT /product="probable lipase (pseudogene)" FT /pseudo FT CDS 969793..972899 FT /class="II.A.5" FT /colour=0 FT /gene="ML0821" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3202c (Best blastx score 725)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS 972938..975898 FT /class="II.A.5" FT /colour=0 FT /gene="ML0822" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3201c (Best blastx score 510)" FT /product="probable ATP-dependent DNA helicase FT (pseudogene)" FT /pseudo FT CDS 976026..977052 FT /class="III.A.2" FT /colour=0 FT /gene="ML0823" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3200c (Best blastx score 571)" FT /product="putative potassium channel (pseudogene)" FT /pseudo FT repeat_region 976796..977949 FT CDS complement(977783..978178) FT /class="I.J.1" FT /colour=9 FT /gene="furB" FT /gene="ML0824" FT /note="Similar to Mycobacterium tuberculosis cosmid y27 FT fur or Rv2359 or MTCY27.21c TR:O05839 (EMBL:Z95208) (130 FT aa) fasta scores: E(): 0, 86.9% id in 130 aa, and to FT Escherichia coli ferric uptake regulation protein fur FT SW:FUR_ECOLI (P06975) (148 aa) fasta scores: E(): 2.1e-13, FT 37.2% id in 129 aa. Contains Pfam match to entry PF01475 FT FUR, Ferric uptake regulator family." FT /product="putative ferric uptake regulatory protein" FT /tb_orthologue="furB" FT misc_feature complement(977810..978169) FT /colour=0 FT /note="Pfam match to entry PF01475 FUR, Ferric uptake FT regulator family, score 185.20, E-value 1e-51" FT CDS complement(978252..978674) FT /class="I.J.1" FT /colour=9 FT /gene="ML0825" FT /note="Similar to Mycobacterium tuberculosis putative FT transcriptional regulator Rv2358 or MTCY27.22c TR:O05840 FT (EMBL:Z95208) (135 aa) fasta scores: E(): 0, 72.9% id in FT 140 aa. Contains a probable helix-turn-helix motif at aa FT 77-98 (Score 1179, SD +3.20) Contains Pfam match to entry FT PF01022 HTH_5, Bacterial regulatory protein, arsR family." FT /product="putative ArsR-family transcriptional regulator" FT /tb_orthologue="Rv2358" FT misc_feature complement(978273..978506) FT /colour=0 FT /note="Pfam match to entry PF01022 HTH_5, Bacterial FT regulatory protein, arsR family, score 79.00, E-value FT 1e-19" FT CDS 978841..980232 FT /class="II.A.3" FT /colour=2 FT /gene="glyS" FT /gene="ML0826" FT /note="Similar to Mycobacterium tuberculosis glycyl-tRNA FT synthetase glyS or Rv2357c or MTCY27.23 or MTCY98.26 FT SW:SYG_MYCTU (O65932) (463 aa) fasta scores: E(): 0, 90.2% FT id in 459 aa and to Thermus aquaticus glycyl-tRNA FT synthetase glyS SW:SYG_THETH (P56206) (505 aa) fasta FT scores: E(): 0, 43.6% id in 512 aa. Contains Pfam match to FT entry PF00587 tRNA-synt_2b, tRNA synthetase class II (G, FT H, P, S and T). Contains PS00017 ATP/GTP-binding site FT motif A (P-loop). Contains PS00179 Aminoacyl-transfer RNA FT synthetases class-II signature 1." FT /product="putative glycyl-tRNA synthase" FT /tb_orthologue="glyS" FT misc_feature 979090..980175 FT /colour=0 FT /note="Pfam match to entry PF00587 tRNA-synt_2b, tRNA FT synthetase class II (G, H, P, S and T), score 6.50, FT E-value 0.00073" FT misc_feature 979402..979425 FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT misc_feature 979426..979488 FT /colour=8 FT /note="PS00179 Aminoacyl-transfer RNA synthetases class-II FT signature 1" FT repeat_region complement(980403..981393) FT CDS complement(981136..981417) FT /class="IV.B.1.c" FT /colour=0 FT /gene="ML0827" FT /note="Possible pseudogene of a Tn3-type element FT transposase" FT /product="putative transposase (pseudogene)" FT /pseudo FT CDS 982489..983339 FT /class="IV.C.2" FT /colour=0 FT /gene="ML0828" FT /note="Possible pseudogene of M. tuberculosis PPE-family FT protein (Best blastx score 102)" FT /product="PPE-family protein (pseudogene)" FT /pseudo FT CDS 983746..984267 FT /class="II.B.5" FT /colour=0 FT /gene="plcB" FT /gene="ML0829" FT /note="Possible pseudogene of M. tuberculosis plcA, B or C FT (Best blastx score 175)" FT /product="phospholipase C precursor (pseudogene)" FT /pseudo FT CDS complement(984296..986298) FT /class="II.C.2" FT /colour=0 FT /gene="ML0830" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2345 (Best blastx score 503)" FT /product="precursor of probable membrane protein FT (pseudogene)" FT /pseudo FT CDS 986474..987763 FT /class="I.F.5" FT /colour=7 FT /gene="dgt" FT /gene="ML0831" FT /note="Similar to Mycobacterium tuberculosis FT deoxyguanosinetriphosphate triphosphohydrolase dgt or FT Rv2344c or MTCY98.13c SW:DGTP_MYCTU (P95240) (431 aa) FT fasta scores: E(): 0, 83.8% id in 421 aa and to many FT hypothetical deoxyguanosine hydrolases. Contains Pfam FT match to entry PF01966 HD, HD domain." FT /product="putative deoxyguanosine triphosphate hydrolase" FT /tb_orthologue="dgt" FT misc_feature 986699..987151 FT /colour=0 FT /note="Pfam match to entry PF01966 HD, HD domain, score FT 6.80, E-value 0.048" FT CDS 987874..988327 FT /class="III.F" FT /colour=0 FT /gene="ML0832" FT /note="Possible pseudogene similar to M. tuberculosis FT paralogue sodC (Best blastx score 163)" FT /product="superoxide dismutase precursor (Cu-Zn) FT (pseudogene)" FT /pseudo FT CDS 988458..990386 FT /EC_number="2.7.7.-" FT /class="II.A.5" FT /colour=2 FT /gene="dnaG" FT /gene="ML0833" FT /note="Similar to Mycobacterium tuberculosis DNA primase FT SW:PRIM_MYCTU (P95239) (639 aa) fasta scores: E(): 0, FT 80.4% id in 642 aa, and to Mycobacterium smegmatis primase FT dnaG TR:O52200 (EMBL:AF027507) (636 aa) fasta scores: E(): FT 0, 76.2% id in 642 aa. Contains Pfam match to entry FT PF01751 Toprim, Toprim domain. Contains Pfam match to FT entry PF01807 zf-CHC2, CHC2 zinc finger." FT /product="DNA primase" FT /tb_orthologue="dnaG" FT misc_feature 988566..988736 FT /colour=0 FT /note="Pfam match to entry PF01807 zf-CHC2, CHC2 zinc FT finger, score 130.30, E-value 3.6e-35" FT misc_feature 989241..989501 FT /colour=0 FT /note="Pfam match to entry PF01751 Toprim, Toprim domain, FT score 65.00, E-value 1.6e-15" FT CDS complement(990401..990703) FT /class="V" FT /colour=10 FT /gene="ML0834" FT /note="Similar to Mycobacterium tuberculosis hypothetical FT protein Rv2342 or MTCY98.11 TR:P95238 (EMBL:Z83860) (85 FT aa) fasta scores: E(): 3e-21, 76.9% id in 78 aa and shows FT weak similarity to Streptomyces coelicolor putative FT secreted protein SCC24.32 TR:CAB86126 (EMBL:AL163003) (108 FT aa) fasta scores: E(): 0.035, 30.5% id in 95 aa." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv2342" FT tRNA 990844..990916 FT /colour=4 FT /note="tRNA Asn anticodon GTT, Cove score 78.91" FT CDS complement(990964..992727) FT /class="I.B.7" FT /colour=0 FT /gene="mez" FT /gene="ML0835" FT /note="Possible pseudogene of M. tuberculosis orthologue FT mez (Best blastx score 299)" FT /product="probable malate oxidoreductase (pseudogene)" FT /pseudo FT CDS 993461..993727 FT /class="V" FT /colour=0 FT /gene="ML0836" FT /note="Possible pseudogene similar to bacterial FT hypothetical proteins e.g. Thermotoga maritima TM0911 FT TR:Q9X013 (EMBL:AE001755)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS 993882..994265 FT /class="V" FT /colour=10 FT /gene="ML0837" FT /ml_unique FT /note="Similar to part of Streptomyces coelicolor putative FT aldolase SCI8.29C TR:Q9RJ24 (EMBL:AL132644) (240 aa) fasta FT scores: E(): 0.001, 32.5% id in 120 aa." FT /product="hypothetical protein" FT CDS complement(995670..996353) FT /EC_number="2.3.1.30" FT /class="I.D.3" FT /colour=7 FT /gene="cysE" FT /gene="ML0838" FT /note="Similar to Mycobacterium tuberculosis putative FT serine acetyltransferase cysE or Rv2335 or MTCY98.0031C FT TR:P95231 (EMBL:Z83860) (229 aa) fasta scores: E(): 0, FT 76.4% id in 229 aa, and to Bacillus subtilis serine FT acetyltransferase cysE or cysA SW:CYSE_BACSU (Q06750) (217 FT aa) fasta scores: E(): 0, 47.1% id in 204 aa. Previously FT sequenced as TR:O32979 (EMBL:Z98741). Contains 4 Pfam FT matches to entry PF00132 hexapep, Bacterial transferase FT hexapeptide (four repeats). Contains PS00101 FT Hexapeptide-repeat containing-transferases signature." FT /product="putative serine acetyltransferase" FT /tb_orthologue="cysE" FT misc_feature complement(995892..995978) FT /colour=8 FT /note="PS00101 Hexapeptide-repeat containing-transferases FT signature" FT misc_feature complement(995898..995951) FT /colour=0 FT /note="Pfam match to entry PF00132 hexapep, Bacterial FT transferase hexapeptide (four repeats), score 7.30, FT E-value 78" FT misc_feature complement(995952..996005) FT /colour=0 FT /note="Pfam match to entry PF00132 hexapep, Bacterial FT transferase hexapeptide (four repeats), score 11.60, FT E-value 19" FT misc_feature complement(996030..996083) FT /colour=0 FT /note="Pfam match to entry PF00132 hexapep, Bacterial FT transferase hexapeptide (four repeats), score 15.90, FT E-value 0.98" FT misc_feature complement(996126..996179) FT /colour=0 FT /note="Pfam match to entry PF00132 hexapep, Bacterial FT transferase hexapeptide (four repeats), score 7.00, FT E-value 86" FT CDS complement(996356..997288) FT /EC_number="4.2.99.8" FT /class="I.D.3" FT /colour=7 FT /gene="cysK" FT /gene="ML0839" FT /note="Similar to Mycobacterium tuberculosis cysteine FT synthase A cysK or Rv2334 or MTCY98.03 SW:CYSK_MYCTU FT (P95230) (310 aa) fasta scores: E(): 0, 85.8% id in 310 FT aa, and to Spinacia oleracea cysteine synthase, FT chloroplast precursor cysK SW:CYSL_SPIOL (P32260) (383 aa) FT fasta scores: E(): 0, 59.2% id in 309 aa. Previously FT sequenced as SW:CYSK_MYCLE (O32978). Contains Pfam match FT to entry PF00291 S_T_dehydratase, Pyridoxal-phosphate FT dependent enzyme. Contains PS00901 Cysteine FT synthase/cystathionine beta-synthase P-phosphate FT attachment site." FT /note="Similar to ML2396" FT /product="putative cysteine synthase" FT /tb_orthologue="cysK" FT misc_feature complement(996404..997270) FT /colour=0 FT /note="Pfam match to entry PF00291 S_T_dehydratase, FT Pyridoxal-phosphate dependent enzyme, score 447.00, FT E-value 1.6e-130" FT misc_feature complement(997136..997192) FT /colour=8 FT /note="PS00901 Cysteine synthase/cystathionine FT beta-synthase P-phosphate attachment site" FT CDS complement(997285..998589) FT /class="VI" FT /colour=8 FT /gene="ML0840" FT /ml_unique FT /note="Unknown function. Previously sequenced as TR:O32977 FT (EMBL:Z98741)." FT /product="hypothetical protein" FT RBS complement(997296..997302) FT /note="possible RBS" FT RBS complement(998600..998606) FT /note="possible RBS" FT RBS 998962..998966 FT /note="possible RBS" FT CDS 998972..999895 FT /class="II.C.2" FT /colour=3 FT /gene="ML0841" FT /ml_unique FT /note="Similar to Mycobacterium paratuberculosis 35 kDa FT protein TR:Q9RAJ4 (EMBL:AJ250887) (307 aa) fasta scores: FT E(): 0, 92.2% id in 307 aa and to Mycobacterium avium FT major membrane protein I mmpI SW:MMP1_MYCAV (Q48899) (307 FT aa) fasta scores: E(): 0, 89.6% id in 308 aa. Previously FT sequenced as SW:MMP1_MYCLE (P46841)." FT /product="major membrane protein I" FT CDS 1000551..1001786 FT /class="V" FT /colour=10 FT /gene="ML0842" FT /note="Similar to Mycobacterium tuberculosis hypothetical FT 44.6 kDa protein Rv1464 or MTV007.11 TR:O53155 FT (EMBL:AL021184) (417 aa) fasta scores: E(): 0, 43.6% id in FT 408 aa and to many other bacterial nifS-homologues, e.g. FT Bacillus subtilis YurW protein yurW TR:O32164 FT (EMBL:Z99120) (406 aa) fasta scores: E(): 0, 46.7% id in FT 405 aa. Previously sequenced as TR:O32975 (EMBL:Z98741). FT Contains Pfam match to entry PF00266 aminotran_5, FT Aminotransferases class-V." FT /note="Similar to ML0117, ML0596 and ML1708" FT /product="conserved hypothetical protein" FT misc_feature 1000653..1001738 FT /colour=0 FT /note="Pfam match to entry PF00266 aminotran_5, FT Aminotransferases class-V, score 23.70, E-value 1.7e-08" FT CDS 1001908..1002395 FT /class="II.C.1" FT /colour=0 FT /gene="lppP" FT /gene="ML0843" FT /note="Possible pseudogene of M. tuberculosis orthologue FT lppP (Best blastx score 294)" FT /product="lipoprotein (pseudogene)" FT /pseudo FT RBS 1002549..1002555 FT /note="possible RBS" FT CDS 1002559..1004112 FT /class="III.A.4" FT /colour=3 FT /gene="narK" FT /gene="ML0844" FT /note="Similar to Mycobacterium tuberculosis putative FT nitrite extrusion protein narK1 or Rv2329c or MTCY3G12.05 FT TR:P71883 (EMBL:Z79702) (515 aa) fasta scores: E(): 0, FT 69.3% id in 488 aa and to Escherichia coli nitrite FT extrusion protein 2 narU SW:NARU_ECOLI (P37758; P77696) FT (462 aa) fasta scores: E(): 0, 36.6% id in 459 aa. There FT is a frameshift near the C-terminus relative to the M. FT tuberculosis homologue. Previously sequenced as TR:O32974 FT (EMBL:Z98741). Contains hydrophobic, probable FT membrane-spanning regions." FT /product="putative nitrite extrusion protein" FT /tb_orthologue="narK1" FT CDS complement(1004100..1004681) FT /class="V" FT /colour=10 FT /gene="ML0845" FT /ml_unique FT /note="Unknown function. Similar to part of some acyl-CoA FT oxidases e.g. Arabidopsis thaliana acyl-CoA oxidase cx2 FT TR:O65201 (EMBL:AF057043) (692 aa) fasta scores: E(): FT 1.5e-06, 35.2% id in 125 aa. Previously sequenced as FT TR:O32973 (EMBL:Z98741) (193 aa) fasta scores: E(): 0, FT 99.5% id in 193 aa." FT /product="hypothetical protein" FT CDS complement(1004959..1005480) FT /class="III.A" FT /colour=0 FT /gene="ML0846" FT /note="Possible pseudogene similar to M. tuberculosis FT paralogue Rv1747 (Best blastx score 127)" FT /product="ABC transporter (pseudogene)" FT /pseudo FT CDS complement(1005522..1005848) FT /class="V" FT /colour=0 FT /gene="ML0847" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2327 (Best blastx score 184)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS 1005906..1008080 FT /class="II.C.4" FT /colour=3 FT /gene="ML0848" FT /note="Similar to Mycobacterium tuberculosis hypothetical FT ABC transporter ATP-binding protein Rv2326c or MTCY3G12.08 FT SW:YN26_MYCTU (P71886) (697 aa) fasta scores: E(): 0, FT 76.9% id in 697 aa. Shares similar domains with many FT ABC-type transporters e.g. Streptomyces roseofulvus ATPase FT component of putative ABC transporter frnD TR:O68910 FT (EMBL:AF058302) (524 aa) fasta scores: E(): 8.4e-14, 31.5% FT id in 495 aa and Synechococcus sp. nitrate transport FT ATP-binding protein NrtD nrtD SW:NRTD_SYNP7(P38046) (274 FT aa) fasta scores: E(): 1.6e-12, 34.8% id in 198 aa. FT Previously sequenced as TR:O32971 (EMBL:Z98741). Contains FT hydrophobic, possible membrane-spanning regions. Contains FT 2 Pfam matches to entry PF00005 ABC_tran, ABC transporter. FT Contains 2 x PS00017 ATP/GTP-binding site motif A FT (P-loop). Contains 2 x PS00211 ABC transporters family FT signature." FT /product="ABC transporter" FT /tb_orthologue="Rv2326c" FT misc_feature 1006821..1007336 FT /colour=0 FT /note="Pfam match to entry PF00005 ABC_tran, ABC FT transporter, score 112.30, E-value 9e-30" FT misc_feature 1006842..1006865 FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT misc_feature 1007109..1007153 FT /colour=8 FT /note="PS00211 ABC transporters family signature" FT misc_feature 1007508..1008038 FT /colour=0 FT /note="Pfam match to entry PF00005 ABC_tran, ABC FT transporter, score 125.00, E-value 1.4e-33" FT misc_feature 1007529..1007552 FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT misc_feature 1007808..1007852 FT /colour=8 FT /note="PS00211 ABC transporters family signature" FT CDS 1008077..1008928 FT /class="V" FT /colour=10 FT /gene="ML0849" FT /note="Similar to Mycobacterium tuberculosis hypothetical FT 30.0 kDa protein Rv2325c or MTCY3G12.09 SW:YN25_MYCTU FT (P71887) (282 aa) fasta scores: E(): 0, 77.3% id in 282 FT aa. Similar other bacterial hypothetical proteins e.g. to FT Bacillus halodurans YbaF protein ybaF TR:Q9Z9J1 FT (EMBL:AB017508) (265 aa) fasta scores: E(): 3.4e-10, 24.5% FT id in 249 aa. Previously sequenced as TR:O32970 FT (EMBL:Z98741)." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv2325c" FT repeat_region 1008755..1009985 FT CDS 1010010..1011174 FT /class="III.A.5" FT /colour=0 FT /gene="ltp1" FT /gene="ML0850" FT /note="Possible pseudogene of M. tuberculosis orthologue FT ltp1 (Best blastx score 347)" FT /product="non-specific lipid transport protein FT (pseudogene)" FT /pseudo FT CDS complement(1011353..1011963) FT /class="I.J.1" FT /colour=0 FT /gene="sirR" FT /gene="ML0851" FT /note="Possible pseudogene of M. tuberculosis orthologue FT sirR (Best blastx score 192)" FT /product="iron-dependent transcriptional repressor FT (pseudogene)" FT /pseudo FT CDS 1012135..1013130 FT /EC_number="2.7.1.26" FT /EC_number="2.7.7.2" FT /class="I.G.9" FT /colour=7 FT /gene="ribF" FT /gene="ML0852" FT /note="Similar to Mycobacterium tuberculosis riboflavin FT kinase ribF or Rv2786c or MTV002.51C TR:O33328 FT (EMBL:AL008967) (331 aa) fasta scores: E(): 0, 87.5% id in FT 327 aa and to many others e.g. Corynebacterium FT ammoniagenes riboflavin biosynthesis protein RibF FT (includes riboflavin kinase) ribF SW:RIBF_CORAM (Q59263) FT (338 aa) fasta scores: E(): 0, 47.7% id in 321 aa. FT Previously sequenced as TR:O32968 (EMBL:Z98741). Contains FT Pfam match to entry PF01687 FAD_Synth, Riboflavin kinase / FT FAD synthetase." FT /product="putative riboflavin kinase" FT /tb_orthologue="ribF" FT misc_feature 1012717..1013124 FT /colour=0 FT /note="Pfam match to entry PF01687 FAD_Synth, Riboflavin FT kinase / FAD synthetase, score 250.80, E-value 1.8e-71" FT RBS 1013236..1013242 FT /note="possible RBS" FT CDS 1013248..1013517 FT /class="II.A.1" FT /colour=2 FT /gene="rpsO" FT /gene="ML0853" FT /note="Similar to Mycobacterium tuberculosis 30S ribosomal FT protein S15 rpsO or Rv2785c or MTV002.50CSW:RS15_MYCTU FT (O33327) (89 aa) fasta scores: E(): 1.1e-32, 92.1% id in FT 89 aa and to Bacillus stearothermophilus 30S ribosomal FT protein S15 rpsO SW:RS15_BACST (P05766) (88 aa) fasta FT scores: E(): 7.7e-22, 62.5% id in 88 aa. Previously FT sequenced as SW:RS15_MYCLE (O32967). Contains Pfam match FT to entry PF00312 Ribosomal_S15, Ribosomal protein S15. FT Contains PS00362 Ribosomal protein S15 signature." FT /product="30S ribosomal protein S15" FT /tb_orthologue="rpsO" FT misc_feature 1013314..1013511 FT /colour=0 FT /note="Pfam match to entry PF00312 Ribosomal_S15, FT Ribosomal protein S15, score 102.30, E-value 1.6e-27" FT misc_feature 1013362..1013454 FT /colour=8 FT /note="PS00362 Ribosomal protein S15 signature" FT CDS 1013839..1016160 FT /EC_number="2.7.7.8" FT /class="II.A.7" FT /colour=2 FT /gene="gpsI" FT /gene="ML0854" FT /note="Similar to Mycobacterium tuberculosis guanosine FT pentaphosphate synthetase / polyribonucleotide FT nucleotidyltransferase gpsI or Rv2783c or MTV002.48C FT TR:O33325 (EMBL:AL008967) (752 aa) fasta scores: E(): 0, FT 90.0% id in 757 aa, to Streptomyces antibioticus guanosine FT pentaphosphate synthetase gpsI TR:Q53597 (EMBL:U19858) FT (740 aa) fasta scores: E(): 0, 67.2% id in 751 aa and to FT Bacillus subtilis polyribonucleotide FT nucleotidyltransferase pnpA or comR SW:PNPA_BACSU (P50849) FT (704 aa) fasta scores: E(): 0, 45.0% id in 723 aa. FT Previously sequenced as TR:O32966 (EMBL:Z98741). Contains FT Pfam match to entry PF00013 KH-domain, KH domain. Contains FT 2 Pfam matches to entry PF01138 RNase_PH, 3' FT exoribonuclease family. Contains Pfam match to entry FT PF00575 S1, S1 RNA binding domain." FT /product="putative polyribonucleotide phosphorylase / FT guanosine pentaphosphate synthetase" FT /tb_orthologue="gpsI" FT misc_feature 1013884..1014525 FT /colour=0 FT /note="Pfam match to entry PF01138 RNase_PH, 3' FT exoribonuclease family, score 157.30, E-value 2.7e-43" FT misc_feature 1014895..1015563 FT /colour=0 FT /note="Pfam match to entry PF01138 RNase_PH, 3' FT exoribonuclease family, score 286.90, E-value 2.6e-82" FT misc_feature 1015642..1015782 FT /colour=0 FT /note="Pfam match to entry PF00013 KH-domain, KH domain, FT score 38.10, E-value 3.4e-10" FT misc_feature 1015831..1016043 FT /colour=0 FT /note="Pfam match to entry PF00575 S1, S1 RNA binding FT domain, score 50.40, E-value 2.7e-12" FT CDS 1016249..1017523 FT /EC_number="3.4.99.-" FT /class="II.B.3" FT /colour=7 FT /gene="pepR" FT /gene="ML0855" FT /note="Similar to Mycobacterium tuberculosis hypothetical FT zinc protease Rv2782c or MTV002.47C SW:YR82_MYCTU (O33324) FT (438 aa) fasta scores: E(): 0, 84.3% id in 421 aa and to FT others, both bacterial and eukaryotic e.g. Homo sapiens FT mitochondrial processing peptidase beta subunit precursor FT pmpcB or mppB SW:MPPB_HUMAN (O75439) (489 aa) fasta FT scores: E(): 0, 30.6% id in 434 aa. Previously sequenced FT as SW:YR82_MYCLE (O32965). Contains Pfam match to entry FT PF00675 Peptidase_M16, Insulinase (Peptidase family M16). FT Contains PS00143 Insulinase family, zinc-binding region FT signature." FT /product="putative zinc protease" FT /tb_orthologue="pepR" FT misc_feature 1016276..1016725 FT /colour=0 FT /note="Pfam match to entry PF00675 Peptidase_M16, FT Insulinase (Peptidase family M16), score 171.90, E-value FT 1e-47" FT misc_feature 1016342..1016413 FT /colour=8 FT /note="PS00143 Insulinase family, zinc-binding region FT signature" FT CDS 1017528..1018537 FT /class="I.B.7" FT /colour=0 FT /gene="ML0856" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2781c (Best blastx score 337)" FT /product="probable oxidoreductase (pseudogene)" FT /pseudo FT repeat_region 1018826..1019844 FT CDS complement(1019690..1020442) FT /class="II.C.4" FT /colour=3 FT /gene="ML0857" FT /note="Similar to Mycobacterium tuberculosis hypothetical FT 26.9 kDa protein Rv2219 or MTCY190.30 SW:YM19_MYCTU FT (Q10405) (250 aa) fasta scores: E(): 0, 80.8% id in 250 FT aa. Similar to Streptomyces coelicolor putative integral FT membrane protein SC3H12.04 TR:CAB90843 (EMBL:AL355740) FT (234 aa) fasta scores: E(): 1.2e-26, 39.6% id in 230 aa FT and to Streptomyces coelicolor putative integral membrane FT protein SC5F7.05 TR:Q9S2P0 (EMBL:AL096872) (234 aa) fasta FT scores: E(): 1.2e-26, 39.6% id in 230 aa. Previously FT sequenced as TR:O32963 (EMBL:Z98741). Contains FT hydrophobic, possible membrane-spanning regions." FT /product="putative membrane protein" FT /tb_orthologue="Rv2219" FT CDS complement(1020469..1021413) FT /class="I.G.3" FT /colour=7 FT /gene="lipA" FT /gene="ML0858" FT /note="Similar to Mycobacterium tuberculosis lipoic acid FT synthetase lipA or Rv2218 or MTCY190.29 SW:LIPA_MYCTU FT (Q10380) (311 aa) fasta scores: E(): 0, 86.5% id in 310 FT aa. Similar to many e.g. Escherichia coli lipoic acid FT synthetase lipA or lip SW:LIPA_ECOLI (P25845; P77595) (321 FT aa) fasta scores: E(): 0, 42.5% id in 294 aa. Previously FT sequenced as SW:LIPA_MYCLE (O32962)." FT /product="putative lipoic acid synthetase" FT /tb_orthologue="lipA" FT CDS complement(1021410..1022117) FT /EC_number="6.-.-.-" FT /class="I.G.3" FT /colour=7 FT /gene="lipB" FT /gene="ML0859" FT /note="Similar to Mycobacterium tuberculosis FT lipoate-protein ligase B lipB or Rv2217 or MTCY190.28 FT SW:LIPB_MYCTU (Q10404) (230 aa) fasta scores: E(): 0, FT 78.4% id in 218 aa. Similar to Escherichia coli FT lipoate-protein ligase B lipB SW:LIPB_ECOLI (P30976) (191 FT aa) blastp score: 84.1 bits, 55% id in 163 aa. Previously FT sequenced as SW:LIPB_MYCLE (O32961). Contains Pfam match FT to entry PF02111 Lipoate_B, Lipoate-protein ligase B. FT Contains PS01313 Lipoate-protein ligase B signature." FT /product="putative lipoate-protein ligase B" FT /tb_orthologue="lipB" FT misc_feature complement(1021572..1022021) FT /colour=0 FT /note="Pfam match to entry PF02111 Lipoate_B, FT Lipoate-protein ligase B, score 323.90, E-value 2.5e-128" FT misc_feature complement(1021839..1021886) FT /colour=8 FT /note="PS01313 Lipoate-protein ligase B signature" FT CDS complement(1022182..1023105) FT /class="V" FT /colour=10 FT /gene="ML0860" FT /note="Similar to Mycobacterium tuberculosis hypothetical FT 31.7 kDa protein Rv2216 or MTCY190.27 SW:YM16_MYCTU FT (Q10403) (301 aa) fasta scores: E(): 0, 84.2% id in 297 FT aa. Similar to many hypothetical proteins from both FT bacteria and eukaryotes e.g. to Drosophila melanogaster FT CG8768 TR:Q9V6F9 (EMBL:AE003821) (297 aa) fasta scores: FT E(): 6.3e-26, 31.3% id in 304 aa. Previously sequenced as FT SW:YM16_MYCLE (O32960)." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv2216" FT RBS complement(1023117..1023122) FT /note="possible RBS" FT CDS complement(1023121..1024713) FT /class="I.B.3" FT /colour=7 FT /gene="ML0861" FT /note="Similar to Mycobacterium tuberculosis FT dihydrolipoamide succinyltransferase component of FT 2-oxoglutarate dehydrogenase complex sucB or Rv2215 or FT MTCY190.26 SW:ODO2_MYCTU (Q10381) (553 aa) fasta scores: FT E(): 0, 81.8% id in 555 aa. Similar to Streptomyces FT seoulensis dihydrolipoamide acetyltransferase pdhB FT TR:Q9Z6I4 (EMBL:AF047034) (612 aa) fasta scores: E(): 0, FT 51.6% id in 614 aa and to many succinyltransferases e.g. FT Escherichia coli dihydrolipoamide succinyltransferase FT component of 2-oxoglutarate dehydrogenase complex sucB FT SW:ODO2_ECOLI (P07016) (404 aa) fasta scores: E(): 0, FT 38.4% id in 409 aa. Previously sequenced s TR:O32959 FT (EMBL:Z98741. Contains 2 Pfam matches to entry PF00364 FT biotin_lipoyl, Biotin-requiring enzymes. Contains Pfam FT match to entry PF00198 2-oxoacid_dh, 2-oxo acid FT dehydrogenases acyltransferase (catalytic domain). FT Contains 2 x PS00189 2-oxo acid dehydrogenases FT acyltransferase component lipoyl binding site." FT /product="putative dihydrolipoamide acyltransferase" FT /tb_orthologue="sucB" FT misc_feature complement(1023139..1023810) FT /colour=0 FT /note="Pfam match to entry PF00198 2-oxoacid_dh, 2-oxo FT acid dehydrogenases acyltransferase (catalytic domain), FT score 253.30, E-value 3.4e-72" FT misc_feature complement(1024138..1024359) FT /colour=0 FT /note="Pfam match to entry PF00364 biotin_lipoyl, FT Biotin-requiring enzymes, score 106.40, E-value 5.6e-28" FT misc_feature complement(1024198..1024287) FT /colour=8 FT /note="PS00189 2-oxo acid dehydrogenases acyltransferase FT component lipoyl binding site" FT misc_feature complement(1024486..1024707) FT /colour=0 FT /note="Pfam match to entry PF00364 biotin_lipoyl, FT Biotin-requiring enzymes, score 110.00, E-value 4.6e-29" FT misc_feature complement(1024546..1024635) FT /colour=8 FT /note="PS00189 2-oxo acid dehydrogenases acyltransferase FT component lipoyl binding site" FT RBS complement(1024723..1024727) FT /note="possible RBS" FT RBS 1024944..1024949 FT /note="possible RBS" FT CDS 1024953..1026743 FT /class="I.B.7" FT /colour=7 FT /gene="ephD" FT /gene="ML0862" FT /note="Similar to Mycobacterium tuberculosis probable FT oxidoreductase ephD or Rv2214c or MTCY190.25C FT SW:EPHD_MYCTU (Q10402) (592 aa) fasta scores: E(): 0, FT 80.4% id in 596 aa. Similar to many putative FT oxidorecuctases e.g. Streptomyces coelicolor putative FT oxidoreductase SCD78.21C TR:Q9ZBX8 (EMBL:AL034355) (585 FT aa) fasta scores: E(): 0, 42.4% id in 582 aa. C-terminal FT half is similar to Pseudomonas paucimobilis C FT alpha-dehydrogenase ligD SW:LIGD_PSEPA (Q01198) (305 aa) FT fasta scores: E(): 1.1e-15, 26.8% id in 269 aa. Previously FT sequenced as TR:O32958 (EMBL:Z98741). Contains Pfam match FT to entry PF00561 abhydrolase, alpha/beta hydrolase fold. FT Contains Pfam match to entry PF00106 adh_short, short FT chain dehydrogenase. Contains PS00061 Short-chain FT dehydrogenases/reductases family signature." FT /product="putative oxidoreductase" FT /tb_orthologue="ephD" FT misc_feature 1025130..1025858 FT /colour=0 FT /note="Pfam match to entry PF00561 abhydrolase, alpha/beta FT hydrolase fold, score 110.40, E-value 3.4e-29" FT misc_feature 1025937..1026494 FT /colour=0 FT /note="Pfam match to entry PF00106 adh_short, short chain FT dehydrogenase, score 191.70, E-value 1.2e-53" FT misc_feature 1026345..1026431 FT /colour=8 FT /note="PS00061 Short-chain dehydrogenases/reductases FT family signature" FT CDS 1026901..1027215 FT /class="VI" FT /colour=8 FT /gene="ML0863" FT /ml_unique FT /note="Unknown function. Previously sequenced as TR:O32957 FT (EMBL:Z98741)." FT /product="hypothetical protein" FT CDS complement(1027286..1028860) FT /EC_number="3.4.11.1" FT /class="II.B.3" FT /colour=7 FT /gene="pepA" FT /gene="ML0864" FT /note="Similar to Mycobacterium tuberculosis probable FT cytosol aminopeptidase pepA or pepB or Rv2213 or FT MTCY190.24 SW:AMPA_MYCTU (Q10401) (515 aa) fasta scores: FT E(): 0, 83.0% id in 522 aa. Similar to many e.g. FT Rickettsia prowazekii cytosol aminopeptidase pepA or rp142 FT SW:AMPA_RICPR (P27888) (500 aa) fasta scores: E(): 0, FT 39.1% id in 384 aa. Previously sequenced as TR:O32956 FT (EMBL:Z98741). Contains Pfam match to entry PF00883 FT Peptidase_M17, Cytosol aminopeptidase family. Contains FT PS00631 Cytosol aminopeptidase signature." FT /product="putative aminopeptidase" FT /tb_orthologue="pepB" FT misc_feature complement(1027406..1028644) FT /colour=0 FT /note="Pfam match to entry PF00883 Peptidase_M17, Cytosol FT aminopeptidase family, score 721.10, E-value 5.2e-213" FT misc_feature complement(1027736..1027759) FT /colour=8 FT /note="PS00631 Cytosol aminopeptidase signature" FT CDS 1029021..1030124 FT /class="I.C.1" FT /colour=7 FT /gene="gcvT" FT /gene="ML0865" FT /note="Similar to Mycobacterium tuberculosis probable FT aminomethyltransferase gcvT or Rv2211c or MTCY190.22 FT SW:GCST_MYCTU (Q10376) (379 aa) fasta scores: E(): 0, FT 84.7% id in 367 aa. Similar to many e.g. Escherichia coli FT aminomethyltransferase gcvT SW:GCST_ECOLI (P27248) (363 FT aa) fasta scores: E(): 0, 39.0% id in 364 aa. Previously FT sequenced as TR:O32955 (EMBL:Z98741). Contains Pfam match FT to entry PF01571 GCV_T, Glycine cleavage T-protein FT (aminomethyl transferase)." FT /product="putative aminomethyltransferase" FT /tb_orthologue="gcvT" FT misc_feature 1029171..1030112 FT /colour=0 FT /note="Pfam match to entry PF01571 GCV_T, Glycine cleavage FT T-protein (aminomethyl transferase), score 500.30, E-value FT 1.5e-146" FT CDS 1030181..1031287 FT /EC_number="2.6.1.42" FT /class="I.D.7" FT /colour=7 FT /gene="ilvE" FT /gene="ML0866" FT /note="Similar to Mycobacterium tuberculosis probable FT branched-chain amino acid aminotransferase ilvE or Rv2210c FT or MTCY190.21C SW:ILVE_MYCTU (Q10399) (368 aa) fasta FT scores: E(): 0, 87.2% id in 368 aa. Similar to many e.g. FT Mus musculus branched-chain amino acid aminotransferase FT (cytosolic) bcat1 or eca39 SW:BCAT_MOUSE (P24288) (386 aa) FT fasta scores: E(): 0, 41.0% id in 329 aa. Previously FT sequenced as SW:ILVE_MYCLE (O32954). Contains Pfam match FT to entry PF01063 aminotran_4, Aminotransferase class IV. FT Contains PS00770 Aminotransferases class-IV signature." FT /product="putative branched-chain-amino-acid transaminase" FT /tb_orthologue="ilvE" FT misc_feature 1030334..1031230 FT /colour=0 FT /note="Pfam match to entry PF01063 aminotran_4, FT Aminotransferase class IV, score 493.50, E-value 1.6e-144" FT misc_feature 1030898..1030999 FT /colour=8 FT /note="PS00770 Aminotransferases class-IV signature" FT CDS complement(1031317..1032059) FT /class="I.G.13" FT /colour=0 FT /gene="cobS" FT /gene="ML0867" FT /note="Possible pseudogene of M. tuberculosis orthologue FT cobS (Best blastx score 348)" FT /product="cobalamin (5'-phosphate) synthase (pseudogene)" FT /pseudo FT CDS complement(1032056..1033111) FT /EC_number="2.4.2.21" FT /class="I.G.13" FT /colour=7 FT /gene="cobT" FT /gene="ML0868" FT /note="Similar to Mycobacterium tuberculosis putative FT nicotinate-nucleotide--dimethylbenzimidazole FT phosphoribosyltransferase cobT or Rv2207 or MTCY190.18 FT SW:COBT_MYCTU (Q10396) (361 aa) fasta scores: E(): 0, FT 81.4% id in 355 aa. Similar to many e.g. Salmonella FT typhimurium nicotinate-nucleotide--dimethylbenzimidazole FT phosphoribosyltransferase cobT SW:COBT_SALTY (Q05603) (356 FT aa) fasta scores: E(): 5.9e-32, 34.1% id in 340 aa. FT Previously sequenced as :O32953 (EMBL:Z98741)." FT /product="putative FT nicotinate-nucleotide-dimethylbenzimidazole FT phosphoribosyltransferase" FT /tb_orthologue="cobT" FT CDS complement(1033201..1033575) FT /class="V" FT /colour=10 FT /gene="ML0869" FT /note="Similar to Mycobacterium tuberculosis hypothetical FT 14.0 kDa protein Rv2206 or MTCY190.17 SW:YM06_MYCTU FT (Q10395) (124 aa) fasta scores: E(): 0, 76.6% id in 124 aa FT and to part of Streptomyces coelicolor putative integral FT membrane protein SC5F7.32 TR:Q9S2R7 (EMBL:AL096872) (251 FT aa) fasta scores: E(): 3.5e-14, 34.7% id in 219 aa. FT Previously sequenced as TR:O32952 (EMBL:Z98741)." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv2206" FT CDS 1033725..1035170 FT /class="V" FT /colour=0 FT /gene="ML0870" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2205c (Best blastx score 131)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT RBS 1035214..1035218 FT /note="possible RBS" FT CDS 1035224..1035580 FT /class="V" FT /colour=10 FT /gene="ML0871" FT /note="Similar to Mycobacterium tuberculosis hypothetical FT 12.5 kDa protein Rv2204c or MTCY190.15C SW:YM04_MYCTU FT (Q10393) (118 aa) fasta scores: E(): 0, 92.4% id in 118 FT aa. Similar to many hypothetical proteins e.g. FT Streptomyces coelicolor hypothetical 12.4 kDa protein FT SC6G10.34C TR:Q9X819 (EMBL:AL049497) (118 aa) fasta FT scores: E(): 0, 73.7% id in 118 aa. Contains Pfam match to FT entry PF01521 HesB-like, HesB-like domain. Contains FT PS01152 Hypothetical hesB/yadR/yfhF family signature." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv2204c" FT misc_feature 1035260..1035577 FT /colour=0 FT /note="Pfam match to entry PF01521 HesB-like, HesB-like FT domain, score 182.00, E-value 9.4e-51" FT misc_feature 1035521..1035574 FT /colour=8 FT /note="PS01152 Hypothetical hesB/yadR/yfhF family FT signature" FT CDS complement(1035615..1036130) FT /class="II.C.4" FT /colour=3 FT /gene="ML0872" FT /note="Similar to Mycobacterium tuberculosis hypothetical FT 24.4 kDa protein Rv2203 or MYCY190.14 SW:YM03_MYCTU FT (Q10392) (230 aa) fasta scores: E(): 0, 61.7% id in 230 FT aa. Contains hydrophobic, possible membrane-spanning FT region." FT /product="putative membrane protein" FT /tb_orthologue="Rv2203" FT CDS 1036449..1037423 FT /class="I.A.1" FT /colour=7 FT /gene="ML0873" FT /note="Similar to Mycobacterium tuberculosis hypothetical FT sugar kinase Rv2202c or MTCY190.13C SW:YM02_MYCTU (Q10391) FT (324 aa) fasta scores: E(): 0, 83.3% id in 324 aa, and to FT Streptomyces coelicolor putative kinase SC6G10.31C FT TR:Q9X816 (EMBL:AL049497) (338 aa) fasta scores: E(): 0, FT 58.9% id in 319 aa. Shows weak similarity to known kinases FT e.g. Bacillus subtilis ribokinase rbsK SW:RBSK_BACSU FT (P36945; P96733) (293 aa) fasta scores: E(): 1.2e-07, FT 26.1% id in 283 aa. Contains Pfam match to entry PF00294 FT pfkB, pfkB family carbohydrate kinase. Contains PS00583 FT pfkB family of carbohydrate kinases signature 1." FT /product="putative carbohydrate kinase" FT /tb_orthologue="cbhK" FT misc_feature 1036587..1036661 FT /colour=8 FT /note="PS00583 pfkB family of carbohydrate kinases FT signature 1" FT misc_feature 1036587..1036670 FT /colour=0 FT /note="Pfam match to entry PF00294 pfkB, pfkB family FT carbohydrate kinase, score 20.30, E-value 1.9e-05" FT CDS complement(1037606..1039639) FT /EC_number="6.3.5.4" FT /class="I.D.2" FT /colour=7 FT /gene="asnB" FT /gene="ML0874" FT /note="Similar to Mycobacterium tuberculosis putative FT asparagine synthetase [glutamine-hydrolyzing] Rv2201 or FT mtcy190.12 SW:ASNH_MYCTU (Q10374) (652 aa) fasta scores: FT E(): 0, 83.3% id in 659 aa and to Bacillus subtilis FT asparagine synthetase [glutamine-hydrolyzing] 1 asnB or FT asn SW:ASNB_BACSU (P54420; O34902) (632 aa) fasta scores: FT E(): 0, 49.0% id in 614 aa. Contains Pfam match to entry FT PF00310 GATase_2, Glutamine amidotransferases class-II. FT Contains Pfam match to entry PF00733 Asn_synthase, FT Asparagine synthase." FT /product="putative asparagine synthetase" FT /tb_orthologue="asnB" FT misc_feature complement(1037657..1038898) FT /colour=0 FT /note="Pfam match to entry PF00733 Asn_synthase, FT Asparagine synthase, score -160.90, E-value 2.9e-07" FT misc_feature complement(1039070..1039417) FT /colour=0 FT /note="Pfam match to entry PF00310 GATase_2, Glutamine FT amidotransferases class-II, score 56.70, E-value 1e-18" FT RBS 1039769..1039774 FT /note="possible RBS" FT CDS 1039782..1040843 FT /EC_number="1.9.3.1" FT /class="I.B.6.a" FT /colour=7 FT /gene="ctaC" FT /gene="ML0875" FT /note="Similar to Mycobacterium tuberculosis probable FT cytochrome C oxidase polypeptide II precursor ctaC or FT Rv2200c or MTCY190.11C SW:COX2_MYCTU (Q10375) (363 aa) FT fasta scores: E(): 0, 85.3% id in 340 aa. Shows weak FT similarity to many cytochrome oxidases e.g. to part of FT Acanthamoeba castellanii cytochrome C oxidase polypeptide FT I+II cox1/2 SW:COX1_ACACA (Q37370) (873 aa) fasta scores: FT E(): 7.1e-10, 28.5% id in 221 aa. Contains hydrophobic, FT probable membrane-spanning regions. Contains Pfam match to FT entry PF00116 COX2, Cytochrome C oxidase subunit II. FT Contains PS00078 CO II and nitrous oxide reductase FT dinuclear copper centers signature." FT /product="putative cytochrome C oxidase chain II" FT /tb_orthologue="ctaC" FT misc_feature 1040460..1040720 FT /colour=0 FT /note="Pfam match to entry PF00116 COX2, Cytochrome C FT oxidase subunit II, score 39.60, E-value 7.8e-10" FT misc_feature 1040511..1040675 FT /colour=8 FT /note="PS00078 CO II and nitrous oxide reductase dinuclear FT copper centers signature" FT CDS 1040896..1041315 FT /class="II.C.4" FT /colour=3 FT /gene="ML0876" FT /note="Similar to Mycobacterium tuberculosis hypothetical FT 14.9 kDa protein Rv2199c or MTCY190.10C SW:YL99_MYCTU FT (Q10406) (139 aa) fasta scores: E(): 0, 91.4% id in 139 aa FT and to Streptomyces coelicolor hypothetical proteins e.g. FT putative integral membrane protein SC6G10.27C TR:Q9X812 FT (EMBL:AL049497) (132 aa) fasta scores: E(): 6.2e-15, 38.8% FT id in 139 aa." FT /product="putative membrane protein" FT /tb_orthologue="Rv2199c" FT CDS 1041502..1042383 FT /class="II.C.4" FT /colour=3 FT /gene="mmpS3" FT /gene="ML0877" FT /note="Similar to Mycobacterium tuberculosis putative FT membrane protein mmpS3 or Rv2198c or MTCY190.09C FT SW:MMS3_MYCTU (Q10390) (299 aa) fasta scores: E(): 0, FT 69.8% id in 308 aa. Contains hydrophobic, possible FT membrane-spanning region." FT /product="putative membrane protein" FT /tb_orthologue="mmpS3" FT CDS 1042383..1043021 FT /class="V" FT /colour=10 FT /gene="ML0878" FT /note="Similar to Mycobacterium tuberculosis hypothetical FT 15.7 kDa protein Rv0008c or MTCY10H4.07C SW:Y008_MYCTU FT (P71577) (214 aa) fasta scores: E(): 0, 62.6% id in 211 aa FT and to Mycobacterium tuberculosis hypothetical 22.5 kDa FT protein Rv2197c or MTCY190.08C SW:YL97_MYCTU (Q10389) (214 FT aa) fasta scores: E(): 0, 62.6% id in 211 aa." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv2197c" FT CDS complement(1043155..1044810) FT /class="I.B.6.a" FT /colour=7 FT /gene="qcrB" FT /gene="ML0879" FT /note="Similar to Mycobacterium tuberculosis FT ubiquinol-cytochrome C reductase cytochrome B subunit qcrB FT or Rv2196 or MTCY190.07 SW:QCRB_MYCTU (Q10388) (549 aa) FT fasta scores: E(): 0, 92.5% id in 548 aa and to FT Streptomyces lividans ubiquinol-cytochrome C reductase FT cytochrome b subunit qcrB SW:QCRB_STRLI (Q9ZFB6) (549 aa) FT fasta scores: E(): 0, 44.8% id in 533 aa. N-terminal half FT is similar to some cytochomes e.g. Bacillus FT stearothermophilus menaquinol-cytochrome C reductase FT cytochrome B subunit qcrB SW:QCRB_BACST (Q45658) (224 aa) FT fasta scores: E(): 1.1e-13, 27.1% id in 207 aa. Contains FT hydrophobic, probable membrane-spanning regions. Contains FT Pfam match to entry PF00033 cytochrome_b_N, Cytochrome FT b(N-terminal)/b6/petB." FT /product="putative cytochrome B" FT /tb_orthologue="qcrB" FT misc_feature complement(1044097..1044747) FT /colour=0 FT /note="Pfam match to entry PF00033 cytochrome_b_N, FT Cytochrome b(N-terminal)/b6/petB, score 32.60, E-value FT 3.5e-08" FT CDS complement(1044807..1045991) FT /class="I.B.6.a" FT /colour=7 FT /gene="qcrA" FT /gene="ML0880" FT /note="Similar to Mycobacterium tuberculosis putative FT ubiquinol-cytochrome C reductase iron-sulfur subunit qcrA FT or Rv2195 or MTCY190.06 SW:QCRA_MYCTU (Q10387) (429 aa) FT fasta scores: E(): 0, 80.9% id in 398 aa. Contains FT hydrophobic, probable membrane-spanning regions. Contains FT PS00200 Rieske iron-sulfur protein signature 2." FT /product="putative Rieske iron-sulphur reductase FT component" FT /tb_orthologue="qcrA" FT misc_feature complement(1044963..1044980) FT /colour=8 FT /note="PS00200 Rieske iron-sulfur protein signature 2" FT CDS complement(1045988..1046857) FT /class="I.B.6.a" FT /colour=7 FT /gene="qcrC" FT /gene="ML0881" FT /note="Similar to Mycobacterium tuberculosis FT ubiquinol-cytochrome C reductase cytochrome C subunit qcrC FT or Rv2194 or MTCY190.05 SW:QCRC_MYCTU (Q10386) (280 aa) FT fasta scores: E(): 0, 84.4% id in 289 aa and to FT Streptomyces coelicolor cytochrome C heme-binding subunit FT SC6G10.23C TR:Q9X808 (EMBL:AL049497) (269 aa) fasta FT scores: E(): 0, 48.9% id in 266 aa. Previously sequenced FT as SW:QCRC_MYCLE (O69583). Contains Pfam match to entry FT PF00034 cytochrome_c, Cytochrome c. Contains 2 x PS00190 FT Cytochrome C family heme-binding site signature." FT /product="putative cytochrome C reductase cytochrome C FT component" FT /tb_orthologue="qcrC" FT misc_feature complement(1046114..1046350) FT /colour=0 FT /note="Pfam match to entry PF00034 cytochrome_c, FT Cytochrome c, score 58.10, E-value 1.9e-13" FT misc_feature complement(1046294..1046311) FT /colour=8 FT /note="PS00190 Cytochrome C family heme-binding site FT signature" FT misc_feature complement(1046597..1046614) FT /colour=8 FT /note="PS00190 Cytochrome C family heme-binding site FT signature" FT CDS complement(1046919..1047527) FT /class="I.B.6.a" FT /colour=7 FT /gene="ctaE" FT /gene="ML0882" FT /note="Similar to Mycobacterium tuberculosis probable FT cytochrome C oxidase polypeptide III ctaE or Rv2193 or FT MTCY190.04 SW:COX3_MYCTU (Q10385) (203 aa) fasta scores: FT E(): 0, 90.1% id in 203 aa. Similar to many e.g. FT Synechococcus vulcanus cytochrome C oxidase polypeptide FT III ctaE SW:COX3_SYNVU (P50677) (201 aa) fasta scores: FT E(): 1.2e-23, 39.0% id in 200 aa. Previously sequenced as FT TR:O69582 (EMBL:AL022602). Contains hydrophobic, probable FT membrane-spanning regions. Contains Pfam match to entry FT PF00510 COX3, Cytochrome c oxidase subunit III." FT /product="cytochrome C oxidase subunit III" FT misc_feature complement(1046928..1047458) FT /colour=0 FT /note="Pfam match to entry PF00510 COX3, Cytochrome c FT oxidase subunit III, score -100.70, E-value 5.1e-10" FT CDS 1047664..1048764 FT /EC_number="2.4.2.18" FT /class="I.D.4" FT /colour=7 FT /gene="trpD" FT /gene="ML0883" FT /note="Similar to Mycobacterium tuberculosis anthranilate FT phosphoribosyltransferase trpD or Rv2192c or MTCY190.03C FT SW:TRPD_MYCTU (Q10382) (370 aa) fasta scores: E(): 0, FT 80.1% id in 362 aa. Similar to many e.g. Streptomyces FT coelicolor anthranilate phosphoribosyltransferase 1 trpD1 FT or SC6G10.20C SW:TRD1_STRCO (O68608) (354 aa) fasta FT scores: E(): 0, 48.8% id in 363 aa. Previously sequenced FT as SW:TRPD_MYCLE (O69581). Contains Pfam match to entry FT PF00591 Glycos_transf_3, glycosyl transferase family." FT /product="putative anthranilate phosphoribosyltransferase" FT /tb_orthologue="trpD" FT misc_feature 1047727..1048191 FT /colour=0 FT /note="Pfam match to entry PF00591 Glycos_transf_3, FT glycosyl transferase family, score 251.50, E-value FT 1.1e-71" FT CDS complement(1048642..1050388) FT /class="II.A.5" FT /colour=0 FT /gene="ML0884" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2191 (Best blastx score 194)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT RBS 1050952..1050956 FT /note="possible RBS" FT CDS 1050963..1052087 FT /class="IV.A" FT /colour=0 FT /gene="ML0885" FT /note="Similar to Mycobacterium tuberculosis putative FT secreted protein Rv2190c or MTCY190.01c or MTV021.23C FT SW:YL90_MYCTU (Q10383; O53524) (385 aa) fasta scores: E(): FT 0, 69.2% id in 386 aa. Similar to many putative secreted FT proteins from Streptomyces coelicolor e.g. putative FT NPL/P60 family secreted protein SCD63A.04C TR:CAB92656 FT (EMBL:AL356832) (347 aa) fasta scores: E(): 1.4e-13, 31.7% FT id in 357 aa. Previously sequenced as TR:O69580 FT (EMBL:AL022602). Contains a probable N-terminal signal FT sequence. Contains Pfam match to entry PF00877 NLPC_P60, FT NLP/P60 family." FT /product="putative secreted protein" FT /tb_orthologue="Rv2190c" FT misc_feature 1051767..1052084 FT /colour=0 FT /note="Pfam match to entry PF00877 NLPC_P60, NLP/P60 FT family, score 125.30, E-value 1.1e-33" FT CDS 1052132..1053286 FT /class="IV.H" FT /colour=7 FT /gene="ML0886" FT /note="Previously sequenced Mycobacterium leprae FT hypothetical protein TR:O69579 (EMBL:AL022602) (384 aa); FT Fasta score E(): 0, 99.7% identity in 384 aa FT overlap(EMBL:AL022602). Also similar to Mycobacterium FT tuberculosis hypothetical protein TR:O53522 FT (EMBL:AL021957) (399 aa); Fasta score E(): 0, 81.5% FT identity in 383 aa overlap(EMBL:AL021957) and several FT Streptomyces coelicolor putative glycosyl transferases FT e.g. TR:Q9X7Z1 (EMBL:AL049497) (412 aa); Fasta score E(): FT 0, 52.2% identity in 387 aa overlap(EMBL:AL049497). FT Contains Pfam match to entry PF00534 Glycos_transf_1, FT Glycosyl transferases group 1." FT /product="possible glycosyl transferase." FT /tb_orthologue="Rv2188c" FT misc_feature 1052681..1053211 FT /colour=0 FT /note="Pfam match to entry PF00534 Glycos_transf_1, FT Glycosyl transferases group 1, score 180.10, E-value FT 3.7e-50" FT CDS complement(1053283..1055085) FT /class="I.A.3" FT /colour=1 FT /gene="ML0887" FT /note="The C-terminus of the predicted product of this CDS FT is identical to the previously sequenced Mycobacterium FT leprae putative long-chain- fatty-acid-CoA ligase FT TR:O69578 (EMBL:AL022602) (351 aa); Fasta score E(): 0, FT 100.0% identity in 351 aa overlap(EMBL:AL022602). Also FT similar to Mycobacterium tuberculosis putative FT long-chain-fatty-acid-CoA ligase, fadD15, TR:O53521 FT (EMBL:AL021957) (600 aa); Fasta score E(): 0, 80.3% FT identity in 600 aa overlap(EMBL:AL021957) and Haemophilus FT influenzae putative long-chain-fatty-acid-CoA ligase (EC FT 6.2.1.3) SW:LCFH_HAEIN (SW:P44446) (607 aa); Fasta score FT E(): 0, 30.3% identity in 587 aa overlap(SW:P44446). FT Contains Pfam match to entry PF00501 AMP-binding, FT AMP-binding enzyme. Contains PS00455 Putative AMP-binding FT domain signature." FT /product="putative long-chain-fatty-acid-CoA ligase" FT /tb_orthologue="fadD15" FT misc_feature complement(1053607..1054947) FT /colour=0 FT /note="Pfam match to entry PF00501 AMP-binding, FT AMP-binding enzyme, score -44.40, E-value 6.1e-13" FT misc_feature complement(1054495..1054530) FT /colour=8 FT /note="PS00455 Putative AMP-binding domain signature" FT RBS complement(1055093..1055097) FT RBS 1055253..1055256 FT CDS 1055260..1055667 FT /class="V" FT /colour=10 FT /gene="ML0888" FT /note="Previously sequenced Mycobacterium leprae FT hypothetical protein TR:O69576 (EMBL:AL022602) (135 aa); FT Fasta score E(): 0, 100.0% identity in 135 aa FT overlap(EMBL:AL022602). Also similar to Mycobacterium FT tuberculosis hypothetical protein TR:O53520 FT (EMBL:AL021957) (129 aa); Fasta score E(): 0, 80.0% FT identity in 130 aa overlap(EMBL:AL021957)." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv2186c" FT CDS 1055786..1056220 FT /class="V" FT /colour=10 FT /gene="ML0889" FT /note="Previously sequenced Mycobacterium leprae FT hypothetical protein TR:O53519 (EMBL:AL021957) (144 aa); FT Fasta score E(): 0, 84.7% identity in 144 aa overlap FT (EMBL:AL021957) (144 aa); Fasta score E(): 0, 84.7% FT identity in 144 aa overlap(EMBL:AL021957). Also highly FT similar to Mycobacterium tuberculosis hypothetical protein FT TR:O53519(EMBL:AL021957)." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv2185c" FT CDS 1056241..1057488 FT /class="III.A.4" FT /colour=3 FT /gene="ML0890" FT /note="Previously sequenced Mycobacterium leprae FT hypothetical protein TR:O69574 (EMBL:AL022602) (415 aa); FT Fasta score E(): 0, 99.8% identity in 415 aa FT overlap(EMBL:AL022602). Also highly similar, in parts, to FT Mycobacterium tuberculosis hypothetical protein TR:O53518 FT (EMBL:AL021957) (379 aa); Fasta score E(): 0, 79.4% FT identity in 378 aa overlap(EMBL:AL021957) and weakly FT similar to many anion-transporting ATPases e.g. FT SW:ARA2_ECOLI (SW:P52145) (583 aa); Fasta score E(): FT 5.2e-07, 29.6% identity in 314 aa overlap(SW:P52145). FT Contains PS00017 ATP/GTP-binding site motif A (P-loop)." FT /product="possible membrane transport ATPase" FT /tb_orthologue="Rv2184c" FT misc_feature 1056274..1056297 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT CDS 1057485..1057877 FT /class="V" FT /colour=10 FT /gene="ML0891" FT /note="Previously sequenced Mycobacterium leprae FT hypothetical protein TR:O69573 (EMBL:AL022602) (130 aa); FT Fasta score E(): 0, 100.0% identity in 130 aa overlap. FT Also highly similar to Mycobacterium tuberculosis FT hypothetical protein Rv2183c TR:O53517 (EMBL:AL021957) FT (131 aa); Fasta score E(): 1.3e-30, 62.6% identity in 131 FT aa overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv2183c" FT RBS 1057956..1057959 FT CDS 1057963..1058697 FT /class="IV.H" FT /colour=7 FT /gene="ML0892" FT /note="Identical to the previously sequenced Mycobacterium FT leprae hypothetical protein TR:O69572 (EMBL:AL022602) (244 FT aa); Fasta score E(): 0, 100.0% identity in 244 aa FT overlap. Also highly similar to Mycobacterium tuberculosis FT hypothetical protein Rv2182c TR:O53516 (EMBL:AL021957) FT (247 aa); Fasta score E(): 0, 79.8% identity in 247 aa FT overlap and several Streptomyces coelicolor putative FT acyltransferases e.g. TR:CAB76086 (EMBL:AL157956) (223 FT aa); Fasta score E(): 0, 51.1% identity in 221 aa overlap. FT Contains Pfam match to entry PF01553 Acyltransferase, FT Acyltransferase." FT /product="possible acyltransferase" FT /tb_orthologue="Rv2182c" FT misc_feature 1058038..1058583 FT /colour=0 FT /note="Pfam match to entry PF01553 Acyltransferase, FT Acyltransferase, score 154.60, E-value 1.6e-42" FT CDS complement(1058705..1059991) FT /class="II.C.4" FT /colour=3 FT /gene="ML0893" FT /note="Identical to the previously sequenced Mycobacterium FT leprae putative integral membrane protein TR:O69571 FT (EMBL:AL022602) (428 aa); Fasta score E(): 0, 100.0% FT identity in 428 aa overlap(EMBL:AL022602). Also highly FT similar to Mycobacterium tuberculosis putative membrane FT protein TR:O53515 (EMBL:AL021957) (427 aa); Fasta score FT E(): 0, 80.0% identity in 431 aa overlap(EMBL:AL021957). FT Contains multiple possible membrane spanning hydrophobic FT domains and a possible N-terminal signal sequence." FT /product="probable conserved integral membrane protein" FT /tb_orthologue="Rv2181" FT CDS 1060118..1060871 FT /class="II.C.4" FT /colour=0 FT /gene="ML0894" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2180c (Best blastx score 217)" FT /product="probable integral membrane protein (pseudogene)" FT /pseudo FT CDS 1061008..1061523 FT /class="V" FT /colour=10 FT /gene="ML0895" FT /note="Identical to the previously sequenced Mycobacterium FT leprae hypothetical protein TR:O69570 (EMBL:AL022602) (171 FT aa); Fasta score E(): 0, 100.0% identity in 171 aa FT overlap(EMBL:AL022602) and highly similar to Mycobacterium FT tuberculosis hypothetical protein TR:O53513 FT (EMBL:AL021957) (168 aa); Fasta score E(): 0, 83.1% FT identity in 166 aa overlap(EMBL:AL021957)." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv2179c" FT CDS 1061587..1062975 FT /class="I.D.4" FT /colour=7 FT /gene="aroG" FT /gene="ML0896" FT /note="Identical to the previously sequenced Mycobacterium FT leprae 3-deoxy-D-arabino-heptulosonate 7-phosphate FT synthase TR:O69569 (EMBL:AL022602) (462 aa); Fasta score FT E(): 0, 100.0% identity in 462 aa overlap(EMBL:AL022602). FT Also highly similar to many others e.g. Mycobacterium FT tuberculosis 3-deoxy-D-arabino-heptulosonate 7-phosphate FT synthase TR:O53512 (EMBL:AL021957) (462 aa); Fasta score FT E(): 0, 89.7% identity in 465 aa overlap(EMBL:AL021957) FT and similar to many plant DAHP synthetase family proteins FT e.g. Arabidopsis thaliana SW:AROF_ARATH (SW:P29976) (525 FT aa); Fasta score E(): 0, 49.0% identity in 441 aa FT overlap(SW:P29976). Contains Pfam match to entry PF01474 FT DAHP_synth_2, Class-II DAHP synthetase family." FT /product="3-deoxy-D-arabino-heptulosonate 7-phosphate FT synthase (DAHP synthetase)." FT /tb_orthologue="aroG" FT misc_feature 1061644..1062957 FT /colour=0 FT /note="Pfam match to entry PF01474 DAHP_synth_2, Class-II FT DAHP synthetase family, score 1026.80, E-value 0" FT CDS complement(1062988..1064190) FT /class="I.J.3" FT /colour=9 FT /gene="ML0897" FT /note="Identical to the previously sequenced Mycobacterium FT leprae putative serine/threonine protein kinase TR:O69568 FT (EMBL:AL022602) (400 aa); Fasta score E(): 0, 99.8% FT identity in 400 aa overlap(EMBL:AL022602). Also highly FT similar to many other putative serine/threonine protein FT kinases e.g. from Mycobacterium tuberculosis: TR:O53510 FT (EMBL:AL021957) (399 aa); Fasta score E(): 0, 75.3% FT identity in 400 aa overlap(EMBL:AL021957) and FT SW:PKNB_MYCTU (SW:P71584) (626 aa); Fasta score E(): FT 2e-31, 39.5% identity in 311 aa overlap(SW:P71584). FT Contains Pfam match to entry PF00069 pkinase, Eukaryotic FT protein kinase domain. Contains PS00108 Serine/Threonine FT protein kinases active-site signature. Contains PS00107 FT Protein kinases ATP-binding region signature." FT /note="Similar to ML0016 and ML0017" FT /product="serine-threonine protein kinase" FT /tb_orthologue="pknL" FT misc_feature complement(1063576..1064175) FT /colour=0 FT /note="Pfam match to entry PF00069 pkinase, Eukaryotic FT protein kinase domain, score 183.90, E-value 2.5e-51" FT misc_feature complement(1063780..1063818) FT /colour=8 FT /note="PS00108 Serine/Threonine protein kinases FT active-site signature" FT misc_feature complement(1064086..1064157) FT /colour=8 FT /note="PS00107 Protein kinases ATP-binding region FT signature" FT CDS 1064343..1064747 FT /class="I.J.1" FT /colour=9 FT /gene="ML0898" FT /note="Identical to the previously sequenced Mycobacterium FT leprae putative transcriptional regulator TR:O69567 FT (EMBL:AL022602) (134 aa); Fasta score E(): 0, 99.3% FT identity in 134 aa overlap(EMBL:AL022602). Also highly FT similar to Mycobacterium tuberculosis hypothetical protein FT TR:O5350 (EMBL:AL021957) (146 aa); Fasta score E(): 0, FT 82.1% identity in 134 aa overlap(EMBL:AL021957). Contains FT a probable helix-turn-helix motif at aa 18-39 (Score 1581, FT SD +4.57)" FT /product="putative DNA-binding protein" FT /tb_orthologue="Rv2175c" FT CDS complement(1064744..1066261) FT /class="II.C.4" FT /colour=3 FT /gene="ML0899" FT /note="Identical to the previously sequenced Mycobacterium FT leprae putative integral membrane protein TR:O69566 FT (EMBL:AL022602) (505 aa); Fasta score E(): 0, 100.0% FT identity in 505 aa overlap(EMBL:AL022602). Also highly FT similar to Mycobacterium tuberculosis putative membrane FT protein TR:O53508 (EMBL:AL021957) (516 aa); Fasta score FT E(): 0, 78.9% identity in 502 aa overlap(EMBL:AL021957). FT Contains multiple possible membrane spanning hydrophobic FT domains." FT /product="probable integral-membrane protein" FT /tb_orthologue="Rv2174" FT CDS complement(1066278..1067324) FT /class="I.G.11" FT /colour=0 FT /gene="idsA2" FT /gene="ML0900" FT /note="Possible pseudogene of M. tuberculosis orthologue FT idsA2 (Best blastx score 561)" FT /product="geranylgeranyl pyrophosphate synthase FT (pseudogene)" FT /pseudo FT misc_feature complement(1066608..1066646) FT /colour=8 FT /note="PS00444 Polyprenyl synthetases signature 2" FT RBS 1067591..1067595 FT CDS 1067600..1068514 FT /class="V" FT /colour=10 FT /gene="ML0901" FT /note="Identical to the previously sequenced Mycobacterium FT leprae hypothetical protein TR:O69565 (EMBL:AL022602) (304 FT aa); Fasta score E(): 0, 99.7% identity in 304 aa FT overlap(EMBL:AL022602). Also highly similar to FT Mycobacterium tuberculosis hypothetical protein TR:O53506 FT (EMBL:AL021957) (301 aa); Fasta score E(): 0, 80.9% FT identity in 299 aa overlap(EMBL:AL021957)." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv2172c" FT CDS complement(1068511..1069230) FT /class="II.C.1" FT /colour=3 FT /gene="ML0902" FT /note="Identical to the previously sequenced Mycobacterium FT leprae putative lipoprotein TR:O69564 (EMBL:AL022602) (239 FT aa); Fasta score E(): 0, 99.6% identity in 239 aa FT overlap(EMBL:AL022602) and also highly similar to another FT putative lipoprotein from Mycobacterium tuberculosis FT TR:O53505 (EMBL:AL021957) (227 aa); Fasta score E(): 0, FT 75.4% identity in 224 aa overlap(EMBL:AL021957). Contains FT a possible N-terminal signal sequence. Contains PS00013 FT Prokaryotic membrane lipoprotein lipid attachment site." FT /product="probable lipoprotein" FT /tb_orthologue="lppM" FT misc_feature complement(1069120..1069152) FT /colour=8 FT /note="PS00013 Prokaryotic membrane lipoprotein lipid FT attachment site" FT CDS complement(1069302..1069934) FT /class="V" FT /colour=10 FT /gene="ML0903" FT /note="Identical to the previously sequenced Mycobacterium FT leprae hypothetical protein TR:O69563 (EMBL:AL022602) (210 FT aa); Fasta score E(): 0, 99.5% identity in 210 aa FT overlap(EMBL:AL022602). Also highly similar to another FT protein of undefined function from Mycobacterium FT tuberculosis TR:O53504 (EMBL:AL021957) (206 aa); Fasta FT score E(): 0, 76.7% identity in 210 aa FT overlap(EMBL:AL021957)." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv2170" FT RBS complement(1069945..1069950) FT RBS 1070241..1070245 FT CDS 1070251..1070655 FT /class="II.C.4" FT /colour=3 FT /gene="ML0904" FT /note="Identical to the previously sequenced Mycobacterium FT leprae hypothetical protein TR:O69562 (EMBL:AL022602) (134 FT aa); Fasta score E(): 0, 100.0% identity in 134 aa FT overlap(EMBL:AL022602). Also highly similar to several FT others e.g. Mycobacterium tuberculosis hypothetical FT protein TR:O53503 (EMBL:AL021957) (134 aa); Fasta score FT E(): 0, 86.6% identity in 134 aa overlap(EMBL:AL021957) FT and Streptomyces coelicolor putative membrane protein FT TR:Q9XAJ8 (EMBL:AL079348) (96 aa); Fasta score E(): 0.22, FT 38.3% identity in 60 aa overlap(EMBL:AL079348). Contains a FT large central possible membrane spanning hydrophobic FT domain." FT /product="probable membrane protein" FT /tb_orthologue="Rv2169c" FT RBS 1071016..1071021 FT CDS 1071028..1071459 FT /class="V" FT /colour=10 FT /gene="ML0905" FT /note="Identical to the previously sequenced Mycobacterium FT leprae hypothetical protein SW:YL66_MYCLE (SW:O69561) (143 FT aa); Fasta score E(): 0, 99.3% identity in 143 aa FT overlap(SW:O69561). Also highly similar to many other FT proteins of undefined function including: Mycobacterium FT tuberculosis SW:YL66_MYCTU (SW:O06211) (143 aa); Fasta FT score E(): 0, 92.3% identity in 142 aa overlap(SW:O06211) FT and Escherichia coli SW:YABB_ECOLI (SW:P22186) (152 aa); FT Fasta score E(): 3.8e-11, 32.0% identity in 147 aa FT overlap(SW:P22186)." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv2166c" FT CDS 1071587..1072705 FT /class="V" FT /colour=10 FT /gene="ML0906" FT /note="Identical over 340 aa to the previously sequenced FT Mycobacterium leprae hypothetical protein SW:YL65_MYCLE FT (SW:O69560) (340 aa); Fasta score E(): 0, 99.7% identity FT in 340 aa overlap(SW:O69560). Also highly similar to many FT other proteins of undefined function including: FT Mycobacterium tuberculosis TR:O06212 (EMBL:Z95388) (396 FT aa); Fasta score E(): 0, 74.2% identity in 376 aa FT overlap(EMBL:Z95388) and Escherichia coli SW:YABC_ECOLI FT (SW:P18595) (313 aa); Fasta score E(): 0, 41.6% identity FT in 298 aa overlap(SW:P18595). Contains Pfam match to entry FT PF01795 DUF36, Domain of unknown function." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv2165c" FT misc_feature 1071704..1072657 FT /colour=0 FT /note="Pfam match to entry PF01795 DUF36, Domain of FT unknown function, score 560.70, E-value 9.8e-165" FT RBS 1072688..1072693 FT CDS 1072702..1073835 FT /class="II.C.4" FT /colour=3 FT /gene="ML0907" FT /note="Identical to the previously sequenced Mycobacterium FT leprae hypothetical protein TR:O69559 (EMBL:AL022602) (377 FT aa); Fasta score E(): 0, 100.0% identity in 377 aa FT overlap(EMBL:AL022602). Also highly similar to FT Mycobacterium tuberculosis TR:O06213 (EMBL:Z95388) (384 FT aa); Fasta score E(): 0, 62.2% identity in 397 aa FT overlap(EMBL:Z95388). Contains a possible membrane FT spanning hydrophobic domain and a possible coiled-coil FT between residues 155..168." FT /product="putative conserved membrane protein" FT /tb_orthologue="Rv2164c" FT CDS 1073832..1075859 FT /class="II.C.3" FT /colour=3 FT /gene="pbpB" FT /gene="ML0908" FT /note="Identical to the previously sequenced Mycobacterium FT leprae penicillin binding protein 2 TR:O69558 FT (EMBL:AL022602) (675 aa); Fasta score E(): 0, 99.9% FT identity in 675 aa overlap(EMBL:AL022602). Also highly FT similar to Mycobacterium tuberculosis hypothetical protein FT TR:O06214 (EMBL:Z95388) (679 aa); Fasta score E(): 0, FT 80.6% identity in 677 aa overlap(EMBL:Z95388) and to many FT other penicillin-binding proteins e.g. Neisseria FT meningitidis SW:PBP2_NEIME (P11882) (581 aa); Fasta score FT E(): 4.3e-29, 32.4% identity in 590 aa overlap(P11882). FT Contains Pfam match to entry PF00905 Transpeptidase, FT Penicillin binding protein transpeptidase domain. Contains FT PS00017 ATP/GTP-binding site motif A (P-loop)." FT /product="penicillin-binding protein 2" FT /tb_orthologue="pbpB" FT misc_feature 1074765..1075778 FT /colour=0 FT /note="Pfam match to entry PF00905 Transpeptidase, FT Penicillin binding protein transpeptidase domain, score FT 417.90, E-value 9.6e-122" FT misc_feature 1075575..1075598 FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT RBS 1075995..1076001 FT CDS 1076001..1077593 FT /class="II.C.3" FT /colour=3 FT /gene="murE" FT /gene="ML0909" FT /note="Identical to the previously sequenced Mycobacterium FT leprae FT UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate FT ligase (EC 6.3.2.13) SW:MURE_MYCLE (AL022602) (530 aa); FT Fasta score E(): 0, 99.8% identity in 530 aa overlap. Also FT highly similar to MurE orthologues from Mycobacterium FT tuberculosis SW:MURE_MYCTU (O06219) (535 aa); Fasta score FT E(): 0, 74.4% identity in 519 aa overlap and Escherichia FT coli SW:MURE_ECOLI (P22188) (494 aa); Fasta score E(): 0, FT 39.1% identity in 493 aa overlap. Contains Pfam match to FT entry PF01225 Mur_ligase, Mur ligase family." FT /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diami FT nopime ligase (meso-diaminopimelate-adding enzyme)." FT /tb_orthologue="murE" FT misc_feature 1076400..1077149 FT /colour=0 FT /note="Pfam match to entry PF01225 Mur_ligase, Mur ligase FT family, score 297.90, E-value 1.3e-85" FT CDS 1077590..1079143 FT /class="II.C.3" FT /colour=3 FT /gene="murF" FT /gene="ML0910" FT /note="Identical to the previously sequenced Mycobacterium FT leprae hypothetical protein FT UDP-N-acetylmuramoylalanyl-D-glutamyl FT -2,6-diaminopimelate--D-alanyl-D-alanyl ligase TR:O69556 FT (EMBL:AL022602) (517 aa); Fasta score E(): 0, 100.0% FT identity in 517 aa overlap(EMBL:AL022602). Also highly FT similar to MurF orthologues from Mycobacterium FT tuberculosis SW:MURF_MYCTU (SW:O06220) (510 aa); Fasta FT score E(): 0, 75.5% identity in 531 aa overlap(SW:O06220) FT and Escherichia coli SW:MURF_ECOLI (SW:P11880) (452 aa); FT Fasta score E(): 1.7e-09, 31.0% identity in 519 aa FT overlap(SW:P11880). Contains Pfam match to entry PF01225 FT Mur_ligase, Mur ligase family." FT /product="UDP-N-acetylmuramoylalanyl-D-glutamyl FT -2,6-diaminopimelate--D-alanyl-D-alanyl ligase FT (D-alanine:D-alanine-adding enzyme)" FT /tb_orthologue="murF" FT misc_feature 1077986..1078726 FT /colour=0 FT /note="Pfam match to entry PF01225 Mur_ligase, Mur ligase FT family, score 267.70, E-value 1.6e-76" FT CDS 1079140..1080219 FT /class="II.C.3" FT /colour=3 FT /gene="mraY" FT /gene="ML0911" FT /note="Identical to the previously sequenced Mycobacterium FT leprae phospho-N-acetylmuramoyl-pentapeptide-transferase FT (EC 2.7.8.13) SW:MRAY_MYCLE (SW:O69555) (359 aa); Fasta FT score E(): 0, 100.0% identity in 359 aa FT overlap(SW:O69555). Also highly similar to FT phospho-N-acetylmuramoyl-pentapeptide-transferases from FT Mycobacterium tuberculosis SW:MRAY_MYCTU (SW:O06221) (359 FT aa); Fasta score E(): 0, 91.1% identity in 359 aa FT overlap(SW:O06221) and Escherichia coli SW:MRAY_ECOLI FT (SW:P15876) (360 aa); Fasta score E(): 6.2e-29, 35.9% FT identity in 343 aa overlap(SW:P15876). Contains multiple FT possible membrane spanning hydrophobic domains and a FT possible N-terminal signal sequence. Contains Pfam match FT to entry PF00953 Glycos_transf_4, Glycosyl transferase." FT /product="phospho-N-acetylmuramoyl-pentapeptidetransferase" FT /tb_orthologue="mraY or murX" FT misc_feature 1079389..1079985 FT /colour=0 FT /note="Pfam match to entry PF00953 Glycos_transf_4, FT Glycosyl transferase, score 274.30, E-value 1.6e-78" FT CDS 1080221..1081693 FT /class="II.C.3" FT /colour=3 FT /gene="murD" FT /gene="ML0912" FT /note="Identical to the previously sequenced Mycobacterium FT leprae UDP-N-acetylmuramoylalanine-D-glutamate ligase FT TR:O69554 (EMBL:AL022602) (490 aa); Fasta score E(): 0, FT 99.8% identity in 490 aa overlap(EMBL:AL022602). Also FT highly similar to orthologues from Mycobacterium FT tuberculosis SW:MURD_MYCTU (SW:O06222) (486 aa); Fasta FT score E(): 0, 75.9% identity in 493 aa overlap(SW:O06222) FT and Bacillus subtilis SW:MURD_BACSU (SW:Q03522) (451 aa); FT Fasta score E(): 7.1e-24, 28.4% identity in 493 aa FT overlap(SW:Q03522). Contains Pfam match to entry PF01225 FT Mur_ligase, Mur ligase family." FT /product="UDP-N-acetylmuramoylalanine-D-glutamate ligase" FT /tb_orthologue="murD" FT misc_feature 1080575..1081243 FT /colour=0 FT /note="Pfam match to entry PF01225 Mur_ligase, Mur ligase FT family, score 251.30, E-value 1.3e-71" FT RBS 1081692..1081696 FT CDS 1081700..1083304 FT /class="III.C" FT /colour=3 FT /gene="ftsW" FT /gene="ML0913" FT /note="Identical to the previously sequenced Mycobacterium FT leprae cell division protein, FtsW, TR:O69553 FT (EMBL:AL022602) (534 aa); Fasta score E(): 0, 99.8% FT identity in 534 aa overlap(EMBL:AL022602). Also highly FT similar many other FtsW-family proteins involved in cell FT wall formation e.g. from Mycobacterium tuberculosis FT FtsW-like protein SW:FTWH_MYCTU (SW:O06223) (524 aa); FT Fasta score E(): 0, 76.5% identity in 528 aa FT overlap(SW:O06223) and Escherichia coli FtsW SW:FTSW_ECOLI FT (SW:P16457) (414 aa); Fasta score E(): 3.6e-31, 37.0% FT identity in 370 aa overlap(SW:P16457). Contains multiple FT possible membrane spanning hydrophobic domains. Contains FT Pfam match to entry PF01098 FTSW_RODA_SPOVE, Cell cycle FT protein. Contains PS00428 Cell cycle proteins ftsW / rodA FT / spoVE signature. Contains PS00041 Bacterial regulatory FT proteins, araC family signature." FT /product="putative cell division protein FtsW" FT /tb_orthologue="ftsW" FT misc_feature 1081910..1082983 FT /colour=0 FT /note="Pfam match to entry PF01098 FTSW_RODA_SPOVE, Cell FT cycle protein, score 564.50, E-value 7e-166" FT misc_feature 1082798..1082926 FT /colour=8 FT /note="PS00041 Bacterial regulatory proteins, araC family FT signature" FT misc_feature 1082849..1082923 FT /colour=8 FT /note="PS00428 Cell cycle proteins ftsW / rodA / spoVE FT signature" FT CDS 1083301..1084524 FT /class="II.C.3" FT /colour=3 FT /gene="murG" FT /gene="ML0914" FT /note="Identical to the previously sequenced Mycobacterium FT leprae FT UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) FT pyrophosphoryl-undecaprenol N-acetylglucosamine FT transferase (EC 2.4.1.-) SW:MURG_MYCLE (SW:O69552) (407 FT aa); Fasta score E(): 0, 99.8% identity in 407 aa FT overlap(SW:O69552). Also highly similar to orthologues FT from Mycobacterium tuberculosis SW:MURG_MYCTU (SW:O06224) FT (410 aa); Fasta score E(): 0, 76.3% identity in 401 aa FT overlap(SW:O06224) and Escherichia coli SW:MURG_ECOLI FT (SW:P17443) (354 aa); Fasta score E(): 4.2e-21, 38.1% FT identity in 365 aa overlap(SW:P17443). Contains a possible FT membrane spanning hydrophobic domain." FT /product="UDP-N-acetylglucosamine--N-acetylmuramyl-(pentape FT pt pyrophosphoryl-undecaprenol N-acetylglucosamine FT transferase" FT /tb_orthologue="murG" FT RBS 1084510..1084514 FT CDS 1084521..1086008 FT /class="II.C.3" FT /colour=3 FT /gene="murC" FT /gene="ML0915" FT /note="Identical, over available sequence, to the FT previously sequenced Mycobacterium leprae FT UDP-N-acetylmuramate-alanine ligase (fragment) TR:O69551 FT (EMBL:AL022602) (235 aa); Fasta score E(): 0, 99.6% FT identity in 235 aa overlap(EMBL:AL022602). Also highly FT similar to UDP-N-acetylmuramate-alanine ligases from FT Mycobacterium tuberculosis SW:MURC_MYCTU (SW:O06225) (494 FT aa); Fasta score E(): 0, 79.8% identity in 494 aa FT overlap(SW:O06225) and Escherichia coli SW:MURC_ECOLI FT (SW:P17952) (491 aa); Fasta score E(): 0, 35.2% identity FT in 477 aa overlap(SW:P17952). Contains Pfam match to entry FT PF01225 Mur_ligase, Mur ligase family." FT /product="UDP-N-acetyl-muramate-alanine ligase" FT /tb_orthologue="murC" FT misc_feature 1084869..1085591 FT /colour=0 FT /note="Pfam match to entry PF01225 Mur_ligase, Mur ligase FT family, score 213.20, E-value 3.8e-60" FT RBS 1085994..1085997 FT CDS 1086005..1087030 FT /class="III.C" FT /colour=3 FT /gene="ML0916" FT /note="Similar to many FtsQ-family proteins thought to be FT involved in septum formation e.g. Mycobacterium FT tuberculosis SW:FTSQ_MYCTU (SW:O06226) (314 aa); Fasta FT score E(): 0, 75.4% identity in 297 aa overlap(SW:O06226) FT and in parts to Corynebacterium glutamicum SW:FTSQ_CORGL FT (SW:O24745) (222 aa); Fasta score E(): 2.3e-13, 26.6% FT identity in 203 aa overlap(SW:O24745). Contains a possible FT membrane spanning hydrophobic domain." FT /product="putative FtsQ-family protein" FT /tb_orthologue="ftsQ" FT RBS 1087281..1087285 FT CDS 1087296..1088435 FT /class="III.C" FT /colour=3 FT /gene="ftsZ" FT /gene="ML0917" FT /note="Highly similar to many proteins essential for cell FT division including: Mycobacterium tuberculosis FT SW:FTSZ_MYCTU (O08378) (379 aa); Fasta score E(): 0, 96.6% FT identity in 379 aa overlap and Streptomyces griseus FT SW:FTSZ_STRGR (P45501) (407 aa); Fasta score E(): 0, 68.7% FT identity in 390 aa overlap. Contains Pfam match to entry FT PF00091 tubulin, Tubulin/FtsZ family. Contains PS01134 FT FtsZ protein signature 1. Contains PS01135 FtsZ protein FT signature 2." FT /product="cell division protein" FT /tb_orthologue="ftsZ" FT misc_feature 1087416..1087520 FT /colour=8 FT /note="PS01134 FtsZ protein signature 1" FT misc_feature 1087467..1087928 FT /colour=0 FT /note="Pfam match to entry PF00091 tubulin, Tubulin/FtsZ FT family, score 62.60, E-value 3.5e-15" FT misc_feature 1087575..1087640 FT /colour=8 FT /note="PS01135 FtsZ protein signature 2" FT CDS 1088458..1089207 FT /class="V" FT /colour=10 FT /gene="ML0918" FT /note="Similar to many proteins of unknown function FT including: Mycobacterium tuberculosis TR:O06227 FT (EMBL:Z95388) (250 aa); Fasta score E(): 0, 60.2% identity FT in 244 aa overlap and Streptomyces griseus SW:YFIH_STRGR FT (P45496) (246 aa); Fasta score E(): 5.8e-21, 38.0% FT identity in 245 aa overlap. Note both of these examples FT are also found downstream of FtsZ." FT /product="conserved hypothetical protein" FT /tb_orthologue="yfiH" FT CDS 1089204..1089986 FT /class="I.J.1" FT /colour=9 FT /gene="ML0919" FT /note="Similar to many proteins of unknown function FT including: Mycobacterium tuberculosis SW:YL48_MYCTU FT (O06228) (258 aa); Fasta score E(): 0, 73.6% identity in FT 258 aa overlap and Corynebacterium glutamicum FT SW:YFZ1_CORGL (O24748) (221 aa); Fasta score E(): 7e-22, FT 41.6% identity in 231 aa overlap. Contains a probable FT helix-turn-helix motif at aa 78-99 (Score 1061, SD +2.80) FT Contains Pfam match to entry PF01168 UPF0001, FT Uncharacterized protein family UPF0001. Contains PS01211 FT Uncharacterized protein family UPF0001 signature." FT /product="possible DNA-binding protein" FT /tb_orthologue="Rv2148c" FT misc_feature 1089261..1089974 FT /colour=0 FT /note="Pfam match to entry PF01168 UPF0001, FT Uncharacterized protein family UPF0001, score 84.00, FT E-value 3.2e-21" FT misc_feature 1089498..1089542 FT /colour=8 FT /note="PS01211 Uncharacterized protein family UPF0001 FT signature" FT CDS 1090054..1090686 FT /class="V" FT /colour=10 FT /gene="ML0920" FT /note="Highly similar to Mycobacterium tuberculosis FT hypothetical protein TR:O06229 (EMBL:Z95388) (241 aa); FT Fasta score E(): 0, 83.5% identity in 218 aa overlap. Note FT the Mycobacterium leprae protein is 23 aa shorter." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv2147c" FT CDS 1090811..1091101 FT /class="II.C.4" FT /colour=3 FT /gene="ML0921" FT /note="Similar to several proteins of unknown function FT e.g. Mycobacterium tuberculosis TR:O06230 (EMBL:Z95388) FT (96 aa); Fasta score E(): 2.8e-32, 88.5% identity in 96 aa FT overlap and Streptomyces coelicolor TR:Q9S2X3 FT (EMBL:AL109663) (94 aa); Fasta score E(): 2.9e-12, 40.7% FT identity in 86 aa overlap. Contains possible membrane FT spanning hydrophobic domains." FT /product="possible membrane protein" FT /tb_orthologue="Rv2146c" FT CDS 1091348..1092148 FT /class="II.C.2" FT /colour=3 FT /gene="ag84" FT /gene="ML0922" FT /note="Identical to the previously sequenced and FT characterised Mycobacterium leprae antigen 84 FT SW:AG84_MYCLE (P46815) (266 aa); Fasta score E(): 0, FT 100.0% identity in 266 aa overlap. Also highly similar to FT Mycobacterium tuberculosis SW:AG84_MYCTU (P46816) (260 FT aa); Fasta score E(): 0, 83.1% identity in 266 aa overlap. FT Contains several possible coiled-coil regions." FT /product="immunogenic protein, antigen 84" FT /tb_orthologue="ag84 or wag31" FT RBS 1092209..1092213 FT CDS 1092221..1092613 FT /class="II.C.4" FT /colour=3 FT /gene="ML0923" FT /note="Highly similar to Mycobacterium tuberculosis FT hypothetical protein TR:O06231 (EMBL:Z95388) (118 aa); FT Fasta score E(): 1.3e-12, 50.4% identity in 129 aa FT overlap. Contains a possible membrane spanning hydrophobic FT domain." FT /product="possible membrane protein" FT CDS complement(1092696..1093050) FT /class="V" FT /colour=0 FT /gene="ML0924" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2143 (Best blastx score 106)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT repeat_region 1092785..1094016 FT RBS 1094364..1094369 FT CDS 1094376..1095812 FT /class="I.D.1" FT /colour=7 FT /gene="glnA" FT /gene="ML0925" FT /note="Highly similar to many glutamine synthetases (EC FT 6.3.1.2) including examples from: Mycobacterium FT tuberculosis SW:GLN1_MYCTU (Q10377) (478 aa); Fasta score FT E(): 0, 91.8% identity in 478 aa overlap and Salmonella FT typhimurium SW:GLNA_SALTY (P06201) (468 aa); Fasta score FT E(): 0, 51.5% identity in 474 aa overlap. Contains Pfam FT match to entry PF00120 gln-synt, Glutamine synthetase. FT Contains PS00182 Glutamine synthetase class-I adenylation FT site. Contains PS00180 Glutamine synthetase signature 1. FT Contains PS00181 Glutamine synthetase putative ATP-binding FT region signature." FT /note="Similar to ML1631" FT /product="glutamine synthase class I (EC 6.3.1.2)." FT /tb_orthologue="glnA1" FT misc_feature 1094430..1095515 FT /colour=0 FT /note="Pfam match to entry PF00120 gln-synt, Glutamine FT synthetase, score 700.60, E-value 1.4e-222" FT misc_feature 1094532..1094588 FT /colour=8 FT /note="PS00180 Glutamine synthetase signature 1" FT misc_feature 1095168..1095215 FT /colour=8 FT /note="PS00181 Glutamine synthetase putative ATP-binding FT region signature" FT misc_feature 1095555..1095593 FT /colour=8 FT /note="PS00182 Glutamine synthetase class-I adenylation FT site" FT CDS complement(1096064..1096432) FT /class="VI" FT /colour=8 FT /gene="ML0926" FT /ml_unique FT /note="Weakly similar, in parts, to Mycobacterium FT tuberculosis SW:Y940_MYCTU (P71569) (288 aa); Fasta score FT E(): 0.0071, 30.4% identity in 112 aa overlap." FT /product="hypothetical protein" FT CDS complement(1096524..1096832) FT /class="VI" FT /colour=8 FT /gene="ML0927" FT /ml_unique FT /note="unknown." FT /product="hypothetical protein" FT repeat_region 1096810..1097839 FT RBS complement(1096839..1096842) FT CDS 1098053..1098337 FT /class="VI" FT /colour=8 FT /gene="ML0928" FT /ml_unique FT /note="unknown function." FT /product="hypothetical protein" FT CDS 1098587..1099186 FT /class="III.A.2" FT /colour=0 FT /gene="mgtC" FT /gene="ML0929" FT /note="Possible pseudogene of M. tuberculosis orthologue FT mgtC (Best blastx score 171)" FT /product="probable magnesium transport ATPase protein C FT (pseudogene)" FT /pseudo FT CDS complement(1099217..1100061) FT /class="I.B.7" FT /colour=0 FT /gene="ML0930" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1812c (Best blastx score 260)" FT /product="probable dehydrogenase (pseudogene)" FT /pseudo FT CDS 1100314..1101169 FT /class="I.H.3" FT /colour=0 FT /gene="ML0931" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1814 (Best blastx score 379)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS 1101352..1101937 FT /class="V" FT /colour=0 FT /gene="ML0932" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1815 (Best blastx score 237)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS 1102568..1103266 FT /class="I.J.1" FT /colour=0 FT /gene="ML0933" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1816 (Best blastx score 526)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS 1103994..1105279 FT /class="I.B.7" FT /colour=0 FT /gene="ML0934" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1817 (Best blastx score 395)" FT /product="flavoprotein (pseudogene)" FT /pseudo FT repeat_region 1105962..1108706 FT CDS complement(1106312..1106410) FT /class="IV.B.1.c" FT /colour=0 FT /gene="ML0935" FT /note="Similar to Agrobacterium tumefaciens transposase FT TR:Q44454 (EMBL:Z18270) (366 aa) fasta scores: E(): FT 0.0002, 51.5% id in 33 aa, and to Pseudomonas putida FT transposase tnpa1 TR:Q9R9U9 (EMBL:AJ245436) (355 aa) fasta FT scores: E(): 0.00088, 48.5% id in 33 aa" FT /pseudo FT /product="transposase (pseudogene)" FT CDS complement(1106679..1107822) FT /class="IV.B" FT /colour=0 FT /gene="ML0936" FT /note="Similar to many e.g. Cryphonectria parasitica FT (Chesnut blight fungus) putative maturase TR:AAF27656 FT (EMBL:AF218567) (778 aa) fasta scores: E(): 7.8e-11, 29.2% FT id in 216 aa" FT /product="putative group II intron maturase-related FT protein" FT /pseudo FT stem_loop complement(1107142..1107195) FT CDS complement(1108027..1108166) FT /class="IV.B.1.c" FT /colour=0 FT /gene="ML0937" FT /note="Similar to Pseudomonas putida transposase tnpa1 FT TR:Q9R9U9 (EMBL:AJ245436) (355 aa) fasta scores: E(): FT 3.4e-05, 47.8% id in 46 aa, and to Agrobacterium FT tumefaciens transposase TR:Q44454 (EMBL:Z18270) (366 aa) FT fasta scores: E(): 0.00026, 41.3% id in 46 aa" FT /pseudo FT /product="transposase (pseudogene)" FT CDS 1109892..1110188 FT /class="VI" FT /colour=8 FT /gene="ML0938" FT /ml_unique FT /note="unknown." FT /product="hypothetical protein" FT RBS 1110178..1110183 FT CDS 1110185..1110427 FT /class="VI" FT /colour=8 FT /gene="ML0939" FT /ml_unique FT /note="unknown." FT /product="hypothetical protein" FT CDS 1110639..1110821 FT /class="V" FT /colour=0 FT /gene="ML0940" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1115 (Best blastx score 139)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS 1111222..1111510 FT /class="V" FT /colour=0 FT /gene="ML0941" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1117 (Best blastx score 151)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(1111588..1112380) FT /class="V" FT /colour=0 FT /gene="ML0942" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1118c (Best blastx score 257)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS 1112745..1114125 FT /class="I.B.5" FT /colour=0 FT /gene="zwf" FT /gene="ML0943" FT /note="Possible pseudogene of M. tuberculosis orthologue FT zwf (Best blastx score 420)" FT /product="glucose-6-phosphate 1-dehydrogenase FT (pseudogene)" FT /pseudo FT CDS 1114160..1115169 FT /class="I.B.5" FT /colour=0 FT /gene="gnd2" FT /gene="ML0944" FT /note="Possible pseudogene of M. tuberculosis orthologue FT gnd2 (Best blastx score 395)" FT /product="6-phosphogluconate dehydrogenase (Gram +) FT (pseudogene)" FT /pseudo FT CDS complement(1115197..1116007) FT /class="III.F" FT /colour=0 FT /gene="ML0945" FT /note="Possible pseudogene of M. tuberculosis orthologue FT bpoB (Best blastx score 308)" FT /product="possible non-heme bromoperoxidase (pseudogene)" FT /pseudo FT RBS 1116225..1116229 FT CDS 1116230..1116553 FT /class="V" FT /colour=10 FT /gene="ML0946" FT /note="Identical to the previously sequenced Mycobacterium FT leprae hypothetical protein TR:O05568 (EMBL:Z94723) (107 FT aa); Fasta score E(): 0, 100.0% identity in 107 aa FT overlap. Also similar to Mycobacterium tuberculosis FT TR:O06818 (EMBL:Z95844) (491 aa); Fasta score E(): FT 0.00066, 38.7% identity in 106 aa overlap, a proposed FT member of the PGRS-family. Contains Pfam match to entry FT PF00934 PE, PE family." FT /product="conserved hypothetical protein" FT misc_feature 1116236..1116526 FT /colour=0 FT /note="Pfam match to entry PF00934 PE, PE family, score FT 0.70, E-value 6.2e-05" FT CDS complement(1117520..1117777) FT /class="VI" FT /colour=8 FT /gene="ML0947" FT /ml_unique FT /note="unknown function." FT /product="hypothetical protein" FT CDS 1118089..1118537 FT /class="V" FT /colour=0 FT /gene="ML0948" FT /note="Possible pseudogene of a protein of unknown FT function from Streptomyces coelicolor FT TR:Q9X7R8(EMBL:AL049863)." FT /product="hypothetical protein (pseudogene)." FT /pseudo FT CDS complement(1118730..1119143) FT /class="VI" FT /colour=8 FT /gene="ML0949" FT /ml_unique FT /note="unknown function." FT /product="hypothetical protein" FT CDS complement(1119155..1119355) FT /class="VI" FT /colour=8 FT /gene="ML0950" FT /ml_unique FT /note="unknown function." FT /product="hypothetical protein" FT CDS 1119349..1120281 FT /class="III.F" FT /colour=0 FT /gene="ML0951" FT /note="Possible pseudogene of M. tuberculosis orthologue FT ephC (Best blastx score 389)" FT /product="possible epoxide hydrolase (pseudogene)" FT /pseudo FT CDS 1120289..1121493 FT /class="V" FT /colour=0 FT /gene="ML0952" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1125 (Best blastx score 383)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS 1121893..1122132 FT /class="VI" FT /colour=8 FT /gene="ML0953" FT /ml_unique FT /note="unknown function." FT /product="hypothetical protein" FT CDS complement(1122419..1123020) FT /class="V" FT /colour=0 FT /gene="ML0954" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1126c (Best blastx score 364). Note the N-terminal FT region of this putative pseudogene was previously FT published TR:O05567." FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(1123017..1124822) FT /class="I.C.1" FT /colour=7 FT /gene="ppdK" FT /gene="ML0955" FT /note="Identical to the previously sequenced Mycobacterium FT leprae pyruvate, phosphate dikinase TR:O05566 FT (EMBL:Z94723) (601 aa); Fasta score E(): 0, 99.8% identity FT in 601 aa overlap. Also highly similar to pyruvate, FT phosphate dikinases from Mycobacterium tuberculosis FT TR:O06579 (EMBL:Z95585) (490 aa); Fasta score E(): 0, FT 71.8% identity in 478 aa overlap and Clostridium FT symbiosumSW:PODK_CLOSY (P22983) (873 aa); Fasta score E(): FT 0, 35.2% identity in 537 aa overlap. Contains Pfam match FT to entry PF00391 PEP-utilizers, PEP-utilizing enzymes. FT Contains Pfam match to entry PF01326 PPDK_N_term, Pyruvate FT phosphate dikinase, PEP/pyruvate binding domain." FT /product="pyruvate, phosphate dikinase" FT /tb_orthologue="ppdK" FT misc_feature complement(1123278..1123484) FT /colour=0 FT /note="Pfam match to entry PF00391 PEP-utilizers, FT PEP-utilizing enzymes, score 57.50, E-value 5.9e-16" FT misc_feature complement(1123659..1124621) FT /colour=0 FT /note="Pfam match to entry PF01326 PPDK_N_term, Pyruvate FT phosphate dikinase, PEP/pyruvate binding domain, score FT 484.20, E-value 1.1e-141" FT CDS 1124867..1125376 FT /class="II.C.4" FT /colour=3 FT /gene="ML0956" FT /note="Almost identical to the previously sequenced FT Mycobacterium leprae hypothetical protein TR:O05565 FT (EMBL:Z94723) (214 aa); Fasta score E(): 0, 100.0% FT identity in 169 aa overlap. Also highly similar to FT Streptomyces coelicolor putative membrane protein FT TR:Q9RKN9 (EMBL:AL133220) (180 aa); Fasta score E(): FT 2.8e-13, 43.8% identity in 137 aa overlap. Contains FT multiple possible membrane spanning hydrophobic domains FT and a possible N-terminal signal sequence." FT /product="putative integral membrane protein." FT CDS complement(1125854..1126228) FT /class="VI" FT /colour=8 FT /gene="ML0957" FT /ml_unique FT /note="unknown function." FT /product="hypothetical protein" FT RBS complement(1126231..1126237) FT CDS 1126239..1126670 FT /class="II.C.5" FT /colour=3 FT /gene="ML0958" FT /note="possible membrane protein which contains a FT potential membrane spanning hydrophobic domain." FT /product="putative membrane protein" FT CDS 1126912..1127265 FT /class="VI" FT /colour=8 FT /gene="ML0959" FT /ml_unique FT /note="unknown function." FT /product="hypothetical protein" FT CDS 1127277..1127736 FT /class="III.A" FT /colour=0 FT /gene="ML0960" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1132 (Best blastx score 183)" FT /product="possible membrane transporter (pseudogene)" FT /pseudo FT CDS complement(1127777..1130059) FT /class="I.D.2" FT /colour=7 FT /gene="metE" FT /gene="ML0961" FT /note="Identical to the previously sequenced Mycobacterium FT leprae 5-methyltetrahydropteroyltriglutamate--homocysteine FT methyltransferase (EC 2.1.1.14) SW:METE_MYCLE (O05564) FT (760 aa); Fasta score E(): 0, 100.0% identity in 760 aa FT overlap. Also highly similar to MetE orthologues from FT Mycobacterium tuberculosis SW:METE_MYCTU (O06584) (759 FT aa); Fasta score E(): 0, 85.0% identity in 759 aa overlap FT and Escherichia coli SW:METE_ECOLI (P25665) (752 aa); FT Fasta score E(): 0, 47.5% identity in 754 aa overlap. FT Contains Pfam match to entry PF01717 Methionine_synt, FT Methionine synthase, vitamin-B12 independent." FT /product="5-methyltetrahydropteroyltriglutamate-homocystein FT methyltransferase." FT /tb_orthologue="metE" FT misc_feature complement(1127795..1128766) FT /colour=0 FT /note="Pfam match to entry PF01717 Methionine_synt, FT Methionine synthase, vitamin-B12 independent, score FT 798.90, E-value 1.9e-236" FT CDS complement(1130782..1131101) FT /class="IV.B.1.c" FT /colour=0 FT /gene="ML0962" FT /note="Possible pseudogene of M. tuberculosis orthologue FT putative transposase Rv1313c (Best blastx score 152)" FT /product="possible transposase (pseudogene)" FT /pseudo FT CDS 1131298..1131762 FT /class="VI" FT /colour=8 FT /gene="ML0963" FT /ml_unique FT /note="unknown function." FT /product="hypothetical protein" FT CDS 1131800..1132057 FT /class="VI" FT /colour=8 FT /gene="ML0964" FT /ml_unique FT /note="unknown function." FT /product="hypothetical protein" FT CDS complement(1133793..1134785) FT /class="V" FT /colour=0 FT /gene="ML0965" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1138c (Best blastx score 217)" FT /product="possible conserved hypothetical protein FT (pseudogene)" FT /pseudo FT CDS complement(1134796..1135289) FT /class="V" FT /colour=0 FT /gene="ML0966" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1139c (Best blastx score 225)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(1135310..1136331) FT /class="I.I" FT /colour=0 FT /gene="ML0967" FT /note="Possible pseudogene of M. tuberculosis orthologue FT pks10 and pks11 (Best blastx score 288)" FT /product="polyketide synthase (chalcone synthase-like) FT (pseudogene)" FT /pseudo FT CDS 1136837..1137335 FT /class="V" FT /colour=0 FT /gene="ML0968" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1140 (Best blastx score 297)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS 1137505..1138598 FT /class="I.A.3" FT /colour=0 FT /gene="ML0969" FT /note="Possible pseudogene of M. tuberculosis orthologue FT mcr (Best blastx score 1092)" FT /product="[alpha]-methyl acyl-CoA racemase (pseudogene)" FT /pseudo FT CDS 1138624..1139418 FT /class="I.B.7" FT /colour=0 FT /gene="ML0970" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1144 (Best blastx score 282)" FT /product="short-chain alcohol dehydrogenase (pseudogene)" FT /pseudo FT CDS 1140053..1140906 FT /class="III.A.6" FT /colour=0 FT /gene="ML0971" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1145 (Best blastx score 305)" FT /product="probable drug transport protein (pseudogene)" FT /pseudo FT CDS 1140947..1141214 FT /class="III.A.6" FT /colour=0 FT /gene="ML0972" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1146 (Best blastx score 131)" FT /product="probable drug transporter (pseudogene)" FT /pseudo FT CDS 1141223..1141417 FT /class="V" FT /colour=0 FT /gene="ML0973" FT /note="Similar to Mycobacterium tuberculosis hypothetical FT protein Rv1147 TR:O06547." FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1147." FT /product="hypothetical protein (pseudogene)." FT /pseudo FT repeat_region 1141351..1142669 FT CDS complement(1142459..1143286) FT /class="V" FT /colour=0 FT /gene="ML0974" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2751 (Best blastx score 348)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(1143307..1144159) FT /class="I.B.7" FT /colour=0 FT /gene="ML0975" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2750 (Best blastx score 216)" FT /product="putative dehydrogenase (pseudogene)" FT /pseudo FT CDS complement(1144200..1144442) FT /class="V" FT /colour=0 FT /gene="ML0976" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2749 (Best blastx score 131)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS 1144496..1147156 FT /class="III.C" FT /colour=3 FT /gene="ftsK" FT /gene="ML0977" FT /note="Identical to the previously sequenced Mycobacterium FT leprae hypothetical protein TR:O05560 (EMBL:Z94723) (886 FT aa); Fasta score E(): 0, 100.0% identity in 886 aa FT overlap. Also highly similar to many proteins involved in FT cell division e.g. Escherichia coli SW:FTSK_ECOLI (P46889) FT (1329 aa); Fasta score E(): 0, 43.9% identity in 531 aa FT overlap and Mycobacterium tuberculosis TR:O33290 FT (EMBL:AL008967) (883 aa); Fasta score E(): 0, 78.1% FT identity in 885 aa overlap. Contains multiple possible FT membrane spanning hydrophobic domains. Contains Pfam match FT to entry PF01580 FtsK_SpoIIIE, FtsK/SpoIIIE family. FT Contains PS00017 ATP/GTP-binding site motif A (P-loop)." FT /product="Cell division protein" FT /tb_orthologue="ftsK" FT misc_feature 1145987..1146592 FT /colour=0 FT /note="Pfam match to entry PF01580 FtsK_SpoIIIE, FT FtsK/SpoIIIE family, score 317.70, E-value 1.4e-91" FT misc_feature 1146119..1146142 FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT CDS complement(1147166..1147708) FT /class="IV.H" FT /colour=7 FT /gene="ML0978" FT /note="Identical to the previously sequenced Mycobacterium FT leprae hypothetical protein TR:O05559 (EMBL:Z94723) (180 FT aa); Fasta score E(): 0, 100.0% identity in 180 aa FT overlap. Also similar to several acetyltransferases FT including: Escherichia coli amino-acid acetyltransferase FT (EC 2.3.1.1) SW:ARGA_ECOLI (P08205) (443 aa); Fasta score FT E(): 0.0013, 26.8% identity in 123 aa overlap and FT Streptomyces coelicolor putative acetyltransferase FT TR:Q9X8N2 (EMBL:AL049628) (169 aa); Fasta score E(): 0, FT 59.8% identity in 164 aa overlap. Highly similar to FT Mycobacterium tuberculosis hypothetical protein Rv2747 FT TR:O33289 (EMBL:AL008967) (174 aa); Fasta score E(): 0, FT 86.2% identity in 174 aa overlap . Contains Pfam match to FT entry PF00583 Acetyltransf, Acetyltransferase (GNAT) FT family." FT /product="putative acetyltransferase" FT /tb_orthologue="Rv2747" FT misc_feature complement(1147307..1147657) FT /colour=0 FT /note="Pfam match to entry PF00583 Acetyltransf, FT Acetyltransferase (GNAT) family, score 36.30, E-value FT 6.8e-07" FT CDS 1147775..1148356 FT /class="I.H.3" FT /colour=1 FT /gene="ML0979" FT /note="Identical to the previously sequenced Mycobacterium FT leprae TR:Q49839 (EMBL:U00019) (193 aa); Fasta score E(): FT 0, 100.0% identity in 193 aa overlap. Also highly similar FT to many phosphatidylglycerophosphate synthases including: FT Mycobacterium tuberculosis Rv2746c TR:O33288 FT (EMBL:AL008967) (209 aa); Fasta score E(): 0, 77.1% FT identity in 188 aa overlap and Bacillus subtilis FT SW:PGSA_BACSU (P46322) (193 aa); Fasta score E(): 5.6e-17, FT 35.8% identity in 193 aa overlap. Contains multiple FT possible membrane spanning hydrophobic domains. Contains FT Pfam match to entry PF01066 CDP-OH_P_transf, CDP-alcohol FT phosphatidyltransferase. Contains PS00379 CDP-alcohol FT phosphatidyltransferases signature." FT /product="CDP-diacylglycerol--glycerol-3-phosphate FT 3-phosphatidyltransferase (EC FT 2.7.8.5)(phosphatidylglycerophosphate synthase)" FT misc_feature 1147904..1148326 FT /colour=0 FT /note="Pfam match to entry PF01066 CDP-OH_P_transf, FT CDP-alcohol phosphatidyltransferase, score 159.90, E-value FT 4.3e-44" FT misc_feature 1147943..1148011 FT /colour=8 FT /note="PS00379 CDP-alcohol phosphatidyltransferases FT signature" FT CDS 1148468..1148751 FT /class="V" FT /colour=0 FT /gene="ML0980" FT /product="hypothetical protein (pseudogene)." FT /pseudo FT CDS 1148891..1149719 FT /class="V" FT /colour=0 FT /gene="ML0981" FT /note="Possible pseudogene of M. tuberculosis orthologue FT 35kd immunogenic protein 35kd_ag (Best blastx score 624)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS 1149754..1150602 FT /class="V" FT /colour=0 FT /gene="ML0982" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2743c (Best blastx score 792)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS 1150700..1150910 FT /class="V" FT /colour=0 FT /gene="ML0983" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2742c (Best blastx score 144)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(1151024..1151473) FT /class="V" FT /colour=10 FT /gene="ML0984" FT /note="Almost identical to the previously sequenced FT Mycobacterium leprae hypothetical protein TR:Q49850 FT (EMBL:U00019) (178 aa); Fasta score E(): 0, 100.0% FT identity in 149 aa overlap. Also highly similar to FT Mycobacterium tuberculosis hypothetical protein TR:O33283 FT (EMBL:AL008967) (149 aa); Fasta score E(): 3e-28, 52.0% FT identity in 150 aa overlap." FT /product="conserved hypothetical protein." FT CDS 1151510..1152688 FT /class="IV.H" FT /colour=7 FT /gene="ML0985" FT /note="Identical to the previously sequenced Mycobacterium FT leprae possible glycosyl transferase TR:Q49841 FT (EMBL:Z94723) (392 aa); Fasta score E(): 0, 100.0% FT identity in 392 aa overlap. Also highly similar to FT Mycobacterium tuberculosis probable transferase Rv2739c FT TR:O33282 (EMBL:AL008967) (388 aa); Fasta score E(): 0, FT 80.9% identity in 388 aa overlap and Erwinia herbicola FT SW:CRTX_ERWHE (Q01330) (413 aa); Fasta score E(): 7.5e-07, FT 26.1% identity in 410 aa overlap." FT /product="possible glycosyltransferase" FT /tb_orthologue="Rv2739c" FT CDS 1152702..1152905 FT /class="V" FT /colour=10 FT /gene="ML0986" FT /note="Highly similar to proteins of undefined function FT from Mycobacterium tuberculosis Rv2738c TR:O33281 FT (EMBL:AL008967) (68 aa); Fasta score E(): 7.9e-24, 85.1% FT identity in 67 aa overlap and Streptomyces coelicolor FT TR:O50484 (EMBL:AL020958) (64 aa); Fasta score E(): FT 2.5e-08, 44.4% identity in 63 aa overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv2738c" FT CDS 1153056..1155191 FT /class="II.A.5" FT /colour=2 FT /gene="recA" FT /gene="ML0987" FT /note="Identical to the previously sequenced and FT characterised Mycobacterium leprae RecA protein involved FT in DNA recombination and the SOS response SW:RECA_MYCLE FT (P35901) (711 aa); Fasta score E(): 0, 100.0% identity in FT 711 aa overlap. Also highly similar to many others e.g. FT Mycobacterium tuberculosis SW:RECA_MYCTU (P26345) (790 FT aa); Fasta score E(): 0, 46.7% identity in 814 aa overlap. FT Note the Mycobacterium leprae RecA protein is known to FT possess an intron which is self-spliced at the FT post-translational level. Contains 2 Pfam matches to entry FT PF00154 recA, recA bacterial DNA recombination proteins. FT Contains PS00321 recA signature. Contains PS00017 FT ATP/GTP-binding site motif A (P-loop). Contains PS00881 FT Protein splicing signature." FT /product="RecA protein" FT misc_feature 1153080..1153670 FT /colour=0 FT /note="Pfam match to entry PF00154 recA, recA bacterial FT DNA recombination proteins, score 495.50, E-value 5e-146" FT misc_feature 1153254..1153277 FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT misc_feature 1154745..1154768 FT /colour=8 FT /note="PS00881 Protein splicing signature" FT misc_feature 1154748..1155140 FT /colour=0 FT /note="Pfam match to entry PF00154 recA, recA bacterial FT DNA recombination proteins, score 263.10, E-value 3.6e-77" FT misc_feature 1154793..1154819 FT /colour=8 FT /note="PS00321 recA signature" FT CDS 1155157..1155672 FT /class="I.J.1" FT /colour=9 FT /gene="recX" FT /gene="ML0988" FT /note="Identical to the previously sequenced Mycobacterium FT leprae putative regulatory protein RecX, though to FT interact with RecA SW:RECX_MYCLE (P37859) (171 aa); Fasta FT score E(): 0, 100.0% identity in 171 aa overlap. Also FT highly similar to RecX from Mycobacterium tuberculosis FT Rv2736c SW:RECX_MYCTU (O33280) (174 aa); Fasta score E(): FT 0, 77.0% identity in 174 aa overlap and several others. FT Note the overlap with upstream recA." FT /product="putative regulatory protein RecX" FT CDS 1155731..1157284 FT /class="V" FT /colour=10 FT /gene="ML0989" FT /note="Identical to the previously sequenced Mycobacterium FT leprae hypothetical protein SW:YR33_MYCLE (Q49842) (516 FT aa); Fasta score E(): 0, 99.8% identity in 516 aa overlap. FT Also highly similar to many other proteins of undefined FT function belonging to the the Pfam UPF0004 family, FT including: Mycobacterium tuberculosis Rv2733c FT SW:YR33_MYCTU (O33238) (512 aa); Fasta score E(): 0, 80.4% FT identity in 509 aa overlap and Bacillus subtilis FT YMCB_BACSU. Contains Pfam match to entry PF00919 UPF0004, FT Uncharacterized protein family UPF0004. Contains PS01278 FT Uncharacterized protein family UPF0004 signature." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv2733c" FT misc_feature 1155785..1157137 FT /colour=0 FT /note="Pfam match to entry PF00919 UPF0004, FT Uncharacterized protein family UPF0004, score 795.30, FT E-value 5e-250" FT misc_feature 1156232..1156294 FT /colour=8 FT /note="PS01278 Uncharacterized protein family UPF0004 FT signature" FT CDS 1157281..1157910 FT /class="II.C.4" FT /colour=3 FT /gene="ML0990" FT /note="Identical to the previously sequenced Mycobacterium FT leprae hypothetical protein TR:Q49834 (EMBL:U00019) (209 FT aa); Fasta score E(): 0, 100.0% identity in 209 aa FT overlap. Also highly similar to Mycobacterium tuberculosis FT Rv2732c TR:O33237 (EMBL:Z98209) (204 aa); Fasta score E(): FT 0, 70.6% identity in 201 aa overlap. Contains possible FT membrane spanning hydrophobic domains." FT /product="possible conserved membrane protein" FT /tb_orthologue="Rv2732c" FT CDS complement(1157913..1159287) FT /class="V" FT /colour=0 FT /gene="ML0991" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2731 (Best blastx score 491)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(1159846..1160079) FT /class="I.J.3" FT /colour=0 FT /gene="ML0992" FT /note="Possible pseudogene of M. tuberculosis orthologue FT pknE (Best blastx score 104)" FT /product="serine-threonine protein kinase (pseudogene)" FT /pseudo FT CDS 1160766..1162375 FT /class="V" FT /colour=0 FT /gene="ML0993" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2729c (Best blastx score 226)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT RBS 1162614..1162619 FT CDS 1162620..1163318 FT /class="V" FT /colour=10 FT /gene="ML0994" FT /note="Identical to the previously sequenced Mycobacterium FT leprae hypothetical protein TR:Q49835 (EMBL:U00019) (232 FT aa); Fasta score E(): 0, 99.6% identity in 232 aa overlap. FT Also highly similar to proteins of undefined function from FT Mycobacterium tuberculosis Rv2728c TR:O33233 (EMBL:Z98209) FT (231 aa); Fasta score E(): 0, 68.5% identity in 232 aa FT overlap and Streptomyces coelicolor TR:O69964 FT (EMBL:AL022268) (237 aa); Fasta score E(): 1.3e-13, 32.9% FT identity in 243 aa overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv2728c" FT CDS 1163315..1164250 FT /class="I.F.5" FT /colour=7 FT /gene="miaA" FT /gene="ML0995" FT /note="Identical to the previously sequenced Mycobacterium FT leprae tRNA delta(2)-isopentenylpyrophosphate transferase FT (EC 2.5.1.8) SW:MIAA_MYCLE (P46811) (311 aa); Fasta score FT E(): 0, 100.0% identity in 311 aa overlap, as well as FT several other IPP transferases e.g. from Mycobacterium FT tuberculosis SW:MIAA_MYCTU (O33232) (314 aa); Fasta score FT E(): 0, 82.2% identity in 314 aa overlap and Escherichia FT coli SW:MIAA_ECOLI (P16384) (316 aa); Fasta score E(): FT 2.2e-27, 35.5% identity in 293 aa overlap. Contains Pfam FT match to entry PF01715 IPPT, IPP transferase." FT /product="tRNA [delta](2)-isopentenylpyrophosphate FT transferase" FT /tb_orthologue="miaA" FT misc_feature 1163429..1164190 FT /colour=0 FT /note="Pfam match to entry PF01715 IPPT, IPP transferase, FT score 478.50, E-value 5.4e-140" FT RBS 1164300..1164305 FT misc_feature 1164317..1165174 FT /colour=0 FT /note="Pfam match to entry PF01678 DAP_epimerase, FT Diaminopimelate epimerase, score 498.80, E-value 4.3e-146" FT CDS 1164317..1165207 FT /class="I.D.2" FT /colour=7 FT /gene="dapF" FT /gene="ML0996" FT /note="Identical to the previously sequenced Mycobacterium FT leprae diaminopimelate epimerase (EC 5.1.1.7) (DAP FT epimerase) involved in the biosynthesis of lysine from FT aspartate semialdehyde SW:DAPF_MYCLE (P46814) (296 aa); FT Fasta score E(): 0, 100.0% identity in 296 aa overlap. FT Also highly similar to DAP epimerases from Mycobacterium FT tuberculosis SW:DAPF_MYCTU (O33231) (289 aa); Fasta score FT E(): 0, 76.0% identity in 292 aa overlap and Escherichia FT coli SW:DAPF_ECOLI (P08885) (274 aa); Fasta score E(): FT 4.2e-13, 32.3% identity in 294 aa overlap. Contains Pfam FT match to entry PF01678 DAP_epimerase, Diaminopimelate FT epimerase. Contains PS01326 Diaminopimelate epimerase FT signature." FT /product="diaminopimelate epimerase" FT /tb_orthologue="dapF" FT misc_feature 1164548..1164592 FT /colour=8 FT /note="PS01326 Diaminopimelate epimerase signature" FT CDS 1165270..1166736 FT /class="II.B.3" FT /colour=7 FT /gene="ML0997" FT /note="Identical to the previously sequenced Mycobacterium FT leprae HflX protein TR:Q49843 (EMBL:U00019) (518 aa); FT Fasta score E(): 0, 100.0% identity in 488 aa overlap. FT Also highly similar to Mycobacterium tuberculosis FT hypothetical protein TR:O33230 (EMBLZ98209:) (495 aa); FT Fasta score E(): 0, 84.5% identity in 485 aa overlap and FT Escherichia coli GTP-binding protein HflX, located within FT the hflA (high frequency of lysogenization) locus FT governing lysis and lysogeny of bacteriophage lambda, FT SW:HFLX_ECOLI (P25519) (426 aa); Fasta score E(): 0, 38.3% FT identity in 428 aa overlap. Contains PS00017 FT ATP/GTP-binding site motif A (P-loop)." FT /product="possible ATP/GTP-binding protein" FT /tb_orthologue="hflX" FT misc_feature 1166068..1166091 FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT CDS 1166930..1168051 FT /class="I.A.3" FT /colour=0 FT /gene="ML0998" FT /note="Possible pseudogene of M. tuberculosis orthologue FT fadE13 (Best blastx score 104)" FT /product="possible acyl-CoA dehydrogenase (pseudogene)" FT /pseudo FT CDS complement(1168380..1168733) FT /class="IV.B.1.c" FT /colour=0 FT /gene="ML0999" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1369c (Best blastx score 128). Similar to several other FT Mycobacterium tuberculosis transposases e.g. FT SW:YK87_MYCTU." FT /product="possible transposase (pseudogene)" FT /pseudo FT CDS 1170353..1170596 FT /class="V" FT /colour=0 FT /gene="ML1000" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2346c (Best blastx score 132)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(1172599..1172874) FT /class="VI" FT /colour=8 FT /gene="ML1001" FT /ml_unique FT /note="Doubful CDS. Similar in the N-terminus to FT Mycobacterium tuberculosis TR:O33227 (EMBL:Z98209) (82 FT aa); Fasta score E(): 4.4e-05, 37.5% identity in 88 aa FT overlap." FT /product="hypothetical protein" FT RBS complement(1172876..1172880) FT RBS 1172911..1172915 FT CDS 1172919..1174982 FT /class="II.C.4" FT /colour=3 FT /gene="ML1002" FT /note="Identical to the previously sequenced Mycobacterium FT leprae hypothetical protein TR:Q49837 (EMBL:U00019) (687 FT aa); Fasta score E(): 0, 99.9% identity in 687 aa overlap. FT Also highly similar to Mycobacterium tuberculosis FT hypothetical protein Rv2721c TR:O07219. Contains a FT possible membrane spanning hydrophobic domain." FT /product="possible conserved membrane protein" FT /tb_orthologue="Rv2721c" FT CDS complement(1175021..1175728) FT /class="I.J.1" FT /colour=9 FT /gene="lexA" FT /gene="ML1003" FT /note="Identical to the previously sequenced Mycobacterium FT leprae LexA protein TR:Q49848 (EMBL:U00019) (235 aa); FT Fasta score E(): 0, 100.0% identity in 235 aa overlap. FT Also highly similar to many other LexA proteins known to FT repress genes in response to DNA damage, as part of the FT SOS response e.g. from Mycobacterium tuberculosis FT TR:Q50765 (EMBL:X91407) (217 aa); Fasta score E(): 0, FT 89.4% identity in 218 aa overlap and Escherichia coli FT SW:LEXA_ECOLI (P03033) (202 aa); Fasta score E(): 2.3e-10, FT 33.5% identity in 215 aa overlap. Note codon 19 represents FT an alternative possible translational start site. Contains FT Pfam match to entry PF00717 Peptidase_S24, Peptidase FT family S24. Contains Pfam match to entry PF01726 FT LexA_DNA_bind, LexA DNA binding domain." FT /product="LexA, SOS repressor protein" FT /tb_orthologue="lexA" FT misc_feature complement(1175060..1175425) FT /colour=0 FT /note="Pfam match to entry PF00717 Peptidase_S24, FT Peptidase family S24, score 104.50, E-value 2e-27" FT misc_feature complement(1175477..1175671) FT /colour=0 FT /note="Pfam match to entry PF01726 LexA_DNA_bind, LexA DNA FT binding domain, score 136.80, E-value 3.8e-37" FT RBS 1175996..1176000 FT CDS 1176008..1176502 FT /class="II.C.4" FT /colour=3 FT /gene="ML1004" FT /note="Identical to the previously sequenced Mycobacterium FT leprae hypothetical protein TR:Q49846 (EMBL:U00019) (164 FT aa); Fasta score E(): 0, 100.0% identity in 164 aa FT overlap. Also highly similar to Mycobacterium tuberculosis FT hypothetical protein Rv2719c TR:O07218 (EMBL:Z96072) (165 FT aa); Fasta score E(): 1.8e-23, 55.2% identity in 163 aa FT overlap. Contains a possible membrane spanning hydrophobic FT domain." FT /product="possible conserved membrane protein" FT CDS 1176664..1177128 FT /class="V" FT /colour=10 FT /gene="ML1005" FT /note="Identical to the previously sequenced Mycobacterium FT leprae hypothetical protein TR:Q49844 (EMBL:U00019) (154 FT aa); Fasta score E(): 0, 100.0% identity in 154 aa FT overlap. Also highly similar to a number of other proteins FT of unknown function e.g. Mycobacterium tuberculosis FT Rv2718c TR:O07217 (EMBL:Z96072) (154 aa); Fasta score E(): FT 0, 92.7% identity in 151 aa overlap and Escherichia coli FT SW:YBAD_ECOLI (P25538) (149 aa); Fasta score E(): 9.7e-21, FT 45.6% identity in 147 aa overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv2718c" FT CDS 1177170..1177655 FT /class="V" FT /colour=10 FT /gene="ML1006" FT /note="Identical to the previously sequenced Mycobacterium FT leprae hypothetical protein TR:Q49838 (EMBL:U00019) (138 FT aa); Fasta score E(): 0, 100.0% identity in 138 aa FT overlap. Note this CDS is 23 aa longer than the previously FT published sequence due to the assignment of the FT translational start site. Also highly similar to a number FT of other proteins of unknown function e.g. Mycobacterium FT tuberculosis Rv2717c TR:O07216 (EMBL:Z96072) (164 aa); FT Fasta score E(): 0, 73.8% identity in 164 aa overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv2717c" FT CDS complement(1177749..1178392) FT /class="V" FT /colour=0 FT /gene="ML1007" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2716 (Best blastx score 190)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(1178457..1179491) FT /class="IV.I" FT /colour=0 FT /gene="ML1008" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2715 (Best blastx score 361)" FT /product="possible hydrolase (pseudogene)" FT /pseudo FT CDS complement(1179519..1180499) FT /class="V" FT /colour=10 FT /gene="ML1009" FT /note="Identical to the previously sequenced Mycobacterium FT leprae hypothetical protein TR:Q49847 (EMBL:U00019) (326 FT aa); Fasta score E(): 0, 100.0% identity in 326 aa FT overlap. Also highly similar to a number of other proteins FT of unknown function e.g. Mycobacterium tuberculosis Rv2714 FT TR:O07213 (EMBL:Z96072) (324 aa); Fasta score E(): 0, FT 89.7% identity in 320 aa overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv2714" FT CDS complement(1180776..1181018) FT /class="VI" FT /colour=8 FT /gene="ML1010" FT /ml_unique FT /note="function unknown." FT /product="hypothetical protein" FT CDS complement(1181058..1181480) FT /class="VI" FT /colour=8 FT /gene="ML1011" FT /ml_unique FT /note="function unknown." FT /product="hypothetical protein" FT CDS complement(1181548..1182968) FT /class="I.B.7" FT /colour=0 FT /gene="ML1012" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2713 (Best blastx score 507)" FT /product="possible dehydrogenase (pseudogene)" FT /pseudo FT CDS complement(1183481..1184173) FT /class="I.J.1" FT /colour=9 FT /gene="ML1013" FT /note="Highly similar to multiple iron-binding regulatory FT proteins such as Mycobacterium tuberculosis SW:IDER_MYCTU FT (Q50495) (230 aa); Fasta score E(): 0, 90.0% identity in FT 230 aa overlap and Corynebacterium diphtheriae FT SW:DTXR_CORDI (P33120) (226 aa); Fasta score E(): 0, 56.1% FT identity in 230 aa overlap. Contains Pfam match to entry FT PF01325 Fe_dep_repress, Iron dependent repressor." FT /product="iron dependent repressor" FT /tb_orthologue="ideR" FT misc_feature complement(1183775..1184161) FT /colour=0 FT /note="Pfam match to entry PF01325 Fe_dep_repress, Iron FT dependent repressor, score 248.00, E-value 1.3e-70" FT RBS complement(1184178..1184185) FT CDS complement(1184330..1185289) FT /class="II.A.7" FT /colour=2 FT /gene="ML1014" FT /note="Identical to the previously sequenced Mycobacterium FT leprae RNA polymerase sigma factor TR:Q59531 (EMBL:U15181) FT (319 aa); Fasta score E(): 0, 100.0% identity in 319 aa FT overlap. Also highly similar to many Sigma-70 family FT proteins e.g. Mycobacterium tuberculosis TR:Q59563 FT (EMBL:U10059) (323 aa); Fasta score E(): 0, 96.2% identity FT in 316 aa overlap and Streptomyces coelicolor FT SW:HRDB_STRCO (P18183) (442 aa); Fasta score E(): 0, 60.7% FT identity in 303 aa overlap. Contains a putative FT helix-turn-helix motif situated between residues 278..299 FT (+5.61 SD). Contains a probable helix-turn-helix motif at FT aa 278-299 (Score 1887, SD +5.61) Contains Pfam match to FT entry PF00140 sigma70, Sigma-70 factor. Contains PS00716 FT Sigma-70 factors family signature 2. Contains PS00715 FT Sigma-70 factors family signature 1." FT /note="Highly similar to the C-terminus of ML1022" FT /product="RNA polymerase sigma factor" FT /tb_orthologue="sigB" FT misc_feature complement(1184369..1185052) FT /colour=0 FT /note="Pfam match to entry PF00140 sigma70, Sigma-70 FT factor, score 388.60, E-value 6e-113" FT misc_feature complement(1184375..1184455) FT /colour=8 FT /note="PS00716 Sigma-70 factors family signature 2" FT misc_feature complement(1184921..1184962) FT /colour=8 FT /note="PS00715 Sigma-70 factors family signature 1" FT RBS complement(1185295..1185299) FT CDS complement(1185456..1185875) FT /class="II.C.4" FT /colour=3 FT /gene="ML1015" FT /note="Almost identical to the previously sequenced FT Mycobacterium leprae hypothetical protein TR:Q49983 FT (EMBL:U15181) (162 aa); Fasta score E(): 0, 100.0% FT identity in 139 aa overlap. Also highly similar to FT Mycobacterium tuberculosis Rv2709 TR:O07210 (EMBL:Z96072) FT (148 aa); Fasta score E(): 0, 70.7% identity in 123 aa FT overlap. Contains possible membrane spanning hydrophobic FT domains." FT /product="possible conserved membrane protein" FT CDS 1185903..1186226 FT /class="V" FT /colour=10 FT /gene="ML1016" FT /note="Identical to the previously sequenced Mycobacterium FT leprae TR:Q49984 (EMBL:U15181) (107 aa); Fasta score E(): FT 0, 99.1% identity in 107 aa overlap. Also highly similar FT to several proteins of undefined function e.g. FT Mycobacterium tuberculosis Rv2707 possible membrane FT protein TR:O07209 (EMBL:Z96072) (82 aa); Fasta score E(): FT 9.2e-29, 87.8% identity in 82 aa overlap." FT /product="conserved hypothetical protein" FT CDS complement(1186213..1187205) FT /class="II.C.4" FT /colour=3 FT /gene="ML1017" FT /note="Identical to the previously sequenced Mycobacterium FT leprae TR:Q49985 (EMBL:U15181) (330 aa); Fasta score E(): FT 0, 100.0% identity in 330 aa overlap. Also highly similar FT to several proteins of undefined function e.g. FT Mycobacterium tuberculosis Rv2707 possible membrane FT protein TR:O07208 (EMBL:Z96072) (324 aa); Fasta score E(): FT 0, 75.4% identity in 325 aa overlap." FT /product="possible conserved integral membrane protein" FT /tb_orthologue="Rv2707" FT CDS complement(1187210..1187425) FT /class="VI" FT /colour=8 FT /gene="ML1018" FT /ml_unique FT /note="unknown function." FT /product="hypothetical protein" FT CDS 1187881..1188089 FT /class="V" FT /colour=0 FT /gene="ML1019" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2706c (Best blastx score 69)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS 1188114..1188447 FT /class="V" FT /colour=0 FT /gene="ML1020" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2705c (Best blastx score 206)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(1188440..1188858) FT /class="V" FT /colour=0 FT /gene="ML1021" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2704 (Best blastx score 153)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(1189185..1190909) FT /class="II.A.7" FT /colour=2 FT /gene="rpoT" FT /gene="ML1022" FT /note="Identical to the previously sequenced Mycobacterium FT leprae RNA polymerase sigma factor, rpoT TR:Q59532 FT (EMBL:U15181) (574 aa); Fasta score E(): 0, 99.8% identity FT in 574 aa overlap. Also highly similar to many other FT proposed sigma factors e.g. Mycobacterium tuberculosis FT SigA (RpoD), RNA polymerase sigma factor Rv2703 FT SW:RPSA_MYCTU (Q60162) (528 aa); Fasta score E(): 0, 80.5% FT identity in 533 aa overlap. Contains a probable FT helix-turn-helix motif at aa 533-554 (Score 1603, SD FT +4.65) Contains Pfam match to entry PF00140 sigma70, FT Sigma-70 factor. Contains PS00716 Sigma-70 factors family FT signature 2. Contains PS00715 Sigma-70 factors family FT signature 1." FT /note="The C-terminus is highly similar to ML1014" FT /product="RNA polymerase sigma factor" FT /tb_orthologue="sigB" FT misc_feature complement(1189224..1189907) FT /colour=0 FT /note="Pfam match to entry PF00140 sigma70, Sigma-70 FT factor, score 464.90, E-value 6.8e-136" FT misc_feature complement(1189230..1189310) FT /colour=8 FT /note="PS00716 Sigma-70 factors family signature 2" FT misc_feature complement(1189776..1189817) FT /colour=8 FT /note="PS00715 Sigma-70 factors family signature 1" FT CDS complement(1190920..1191894) FT /class="I.A.1" FT /colour=7 FT /gene="ppgK" FT /gene="ML1023" FT /note="Identical to the previously sequenced Mycobacterium FT leprae polyphosphate glucokinase (EC 2.7.1.63) FT SW:PPGK_MYCLE (Q49988) (324 aa); Fasta score E(): 0, 99.7% FT identity in 324 aa overlap catalysing the phosphorylation FT of glucose thereby generating D-glucose 6-phosphate. Also FT highly similar to Mycobacterium tuberculosis SW:PPGK_MYCTU FT (Q59568) (265 aa); Fasta score E(): 0, 82.8% identity in FT 262 aa overlap. Note the predicted translational start FT site for this CDS maybe incorrect due to the overlap with FT CDS ML1024." FT /product="polyphosphate glucokinase" FT /tb_orthologue="ppgK" FT CDS 1191843..1192718 FT /class="IV.I" FT /colour=7 FT /gene="suhB" FT /gene="ML1024" FT /note="Identical to the previously sequenced Mycobacterium FT leprae extragenic suppressor protein SuhB homologue FT SW:SUHB_MYCLE (P46813) (291 aa); Fasta score E(): 0, 99.7% FT identity in 291 aa overlap. Also highly similar to many FT other members of the inositol monophosphatase family FT including: Mycobacterium tuberculosis, Rv2701c or ShuB, FT SW:SUHB_MYCTU (O07203) (290 aa); Fasta score E(): 0, 77.6% FT identity in 294 aa overlap and Escherichia coli ShuB, FT SW:SUHB_ECOLI (P22783) (267 aa); Fasta score E(): 1.6e-18, FT 34.7% identity in 239 aa overlap, shown to suppress the FT effect of a temperature-sensitive mutation in E. coli. FT Note the predicted translational start site for this CDS FT maybe incorrect due to the overlap with CDS ML1023. FT Contains Pfam match to entry PF00459 inositol_P, Inositol FT monophosphatase family. Contains PS00629 Inositol FT monophosphatase family signature 1. Contains PS00630 FT Inositol monophosphatase family signature 2." FT /product="putative inositol monophosphatase." FT /tb_orthologue="suhB" FT misc_feature 1192080..1192598 FT /colour=0 FT /note="Pfam match to entry PF00459 inositol_P, Inositol FT monophosphatase family, score 266.10, E-value 4.7e-76" FT misc_feature 1192143..1192184 FT /colour=8 FT /note="PS00629 Inositol monophosphatase family signature FT 1" FT misc_feature 1192542..1192586 FT /colour=8 FT /note="PS00630 Inositol monophosphatase family signature FT 2" FT CDS complement(1192722..1193372) FT /class="II.C.2" FT /colour=3 FT /gene="ML1025" FT /note="Identical to the previously sequenced Mycobacterium FT leprae hypothetical protein TR:Q49989 (EMBL:U15181) (216 FT aa); Fasta score E(): 0, 99.5% identity in 216 aa overlap. FT Also highly similar to Mycobacterium tuberculosis FT hypothetical protein Rv2700 TR:O07202 (EMBL:Z96072) (216 FT aa); Fasta score E(): 0, 82.4% identity in 216 aa overlap. FT Contains a possible N-terminal signal sequence." FT /product="possible secreted protein" FT /tb_orthologue="Rv2700" FT CDS 1193568..1193870 FT /class="V" FT /colour=10 FT /gene="ML1026" FT /note="Identical to the previously sequenced Mycobacterium FT leprae hypothetical protein TR:Q4998. Also highly similar FT to hypothetical proteins from Mycobacterium tuberculosis FT Rv2699c TR:O07201 (EMBL:Z96072) (100 aa); Fasta score E(): FT 0, 96.0% identity in 100 aa overlap and Streptomyces FT coelicolor TR:O54130 (EMBL:AL021530) (98 aa); Fasta score FT E(): 3.4e-26, 70.4% identity in 98 aa overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv2699c" FT CDS complement(1193875..1194348) FT /class="II.C.4" FT /colour=3 FT /gene="ML1027" FT /note="Identical to the previously sequenced Mycobacterium FT leprae hypothetical protein TR:Q49991 (EMBL:U15181) (157 FT aa); Fasta score E(): 0, 99.4% identity in 157 aa overlap. FT Also highly similar to proteins of unknown function from FT Mycobacterium tuberculosis Rv2698 TR:O07200 (EMBL:Z96072) FT (161 aa); Fasta score E(): 0, 78.9% identity in 161 aa FT overlap and Streptomyces coelicolor TR:O54132 FT (EMBL:AL021530) (154 aa); Fasta score E(): 1.1e-08, 33.6% FT identity in 149 aa overlap. Contains possible membrane FT spanning hydrophobic domains. Contains a probable FT helix-turn-helix motif at aa 87-108 (Score 1009, SD FT +2.62)" FT /product="possible membrane protein" FT /tb_orthologue="Rv2698" FT CDS 1194374..1194838 FT /class="I.F.3" FT /colour=7 FT /gene="dut" FT /gene="ML1028" FT /note="Identical to the previously sequenced Mycobacterium FT leprae deoxyuridine 5'-triphosphate nucleotidohydrolase FT (EC 3.6.1.23) SW:DUT_MYCLE (Q49992) (155 aa); Fasta score FT E(): 0, 100.0% identity in 154 aa overlap. Also highly FT similar to many other dUTPases, involved in nucleotide FT metabolism, including Mycobacterium tuberculosis FT SW:DUT_MYCTU (O07199) (154 aa); Fasta score E(): 0, 90.3% FT identity in 154 aa overlap and Escherichia coli FT SW:DUT_ECOLI (P06968) (461 aa); BlastP Expect 8.8. FT Contains Pfam match to entry PF00692 dUTPase, dUTPase." FT /product="probable deoxyuridine triphosphatase" FT /tb_orthologue="dut" FT misc_feature 1194407..1194820 FT /colour=0 FT /note="Pfam match to entry PF00692 dUTPase, dUTPase, score FT 103.50, E-value 4.2e-27" FT CDS 1194835..1195656 FT /class="V" FT /colour=10 FT /gene="ML1029" FT /note="Identical to the previously sequenced Mycobacterium FT leprae hypothetical protein TR:Q49993 (EMBL:U15181) (273 FT aa); Fasta score E(): 0, 99.6% identity in 273 aa overlap. FT Also highly similar to Mycobacterium tuberculosis FT hypothetical protein Rv2696c TR:O07198 (EMBL:Z96072) (259 FT aa); Fasta score E(): 0, 70.6% identity in 262 aa FT overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv2696c" FT CDS complement(1195701..1196399) FT /class="V" FT /colour=10 FT /gene="ML1030" FT /note="Identical to the previously sequenced Mycobacterium FT leprae hypothetical protein TR:Q49994 (EMBL:U15181) (232 FT aa); Fasta score E(): 0, 99.6% identity in 232 aa overlap. FT Also highly similar to Mycobacterium tuberculosis FT hypothetical protein Rv2695 TR:O07197 (EMBL:Z96072) (235 FT aa); Fasta score E(): 0, 76.5% identity in 230 aa FT overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv2695" FT CDS 1196608..1196934 FT /class="V" FT /colour=0 FT /gene="ML1031" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2694c (Best blastx score 124)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS 1197131..1197553 FT /class="V" FT /colour=0 FT /gene="ML1032" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2693c (Best blastx score 118)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(1197735..1198009) FT /class="III.A.2" FT /colour=0 FT /gene="ML1033" FT /note="Possible pseudogene of M. tuberculosis orthologue FT trkB (Best blastx score 154)" FT /product="putative potassium uptake protein (pseudogene)" FT /pseudo FT CDS 1198007..1198482 FT /class="V" FT /colour=0 FT /gene="ML1034" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2687c (Best blastx score 207)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS 1198510..1199220 FT /class="II.C.4" FT /colour=0 FT /gene="ML1035" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2686c (Best blastx score 229)" FT /product="possible membrane protein (pseudogene)" FT /pseudo FT CDS complement(1199237..1200526) FT /class="III.A.4" FT /colour=3 FT /gene="ML1036" FT /note="Identical to the previously sequenced Mycobacterium FT leprae probable membrane transport protein SW:AG45_MYCLE FT (P46838) (429 aa); Fasta score E(): 0, 99.8% identity in FT 429 aa overlap. Also highly similar to Mycobacterium FT tuberculosis probable integral-membrane transport protein FT SW:YQ84_MYCTU (Z96072) (429 aa); Fasta score E(): 0, 74.3% FT identity in 428 aa overlap and Escherichia coli arsenical FT pump membrane protein SW:ARSB_ECOLI (P37310) (429 aa); FT Fasta score E(): 1.2e-17, 23.2% identity in 435 aa FT overlap. Contains multiple possible membrane spanning FT hydrophobic domains." FT /product="probable membrane transport protein" FT /tb_orthologue="arsB" FT CDS complement(1200579..1201133) FT /class="V" FT /colour=10 FT /gene="ML1037" FT /note="Identical to the previously sequenced Mycobacterium FT leprae hypothetical protein TR:Q49999 (EMBL:U15181) (184 FT aa); Fasta score E(): 0, 99.5% identity in 184 aa overlap. FT Also highly similar to Mycobacterium tuberculosis FT hypothetical protein Rv2683 TR:O07185 (EMBL:Z96072) (165 FT aa); Fasta score E(): 0, 73.8% identity in 164 aa overlap. FT Contains 2 Pfam matches to entry PF00571 CBS, CBS domain." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv2683" FT misc_feature complement(1200588..1200746) FT /colour=0 FT /note="Pfam match to entry PF00571 CBS, CBS domain, score FT 31.90, E-value 1.5e-05" FT misc_feature complement(1200852..1201013) FT /colour=0 FT /note="Pfam match to entry PF00571 CBS, CBS domain, score FT 27.80, E-value 0.00025" FT CDS 1201215..1203146 FT /class="I.G.11" FT /colour=7 FT /gene="dxs" FT /gene="ML1038" FT /note="Identical to the previously sequenced Mycobacterium FT leprae probable 1-deoxyxylulose-5-phosphate synthase (DXP FT synthase) SW:DXS_MYCL (Q50000) (643 aa); Fasta score E(): FT 0, 100.0% identity in 643 aa overlapE. Also highly similar FT to many members of the transketolase family including DXP FT synthases from Mycobacterium tuberculosis Rv2682c FT SW:DXS_MYCTU (O07184) (638 aa); Fasta score E(): 0, 86.4% FT identity in 632 aa overlap and Escherichia coli FT SW:DXS_ECOLI (P77488) (619 aa); Fasta score E(): 0, 39.2% FT identity in 625 aa overlap. Contains PS00801 Transketolase FT signature 1. Contains PS00802 Transketolase signature 2." FT /product="1-deoxy-D-xylulose 5-phosphate synthase (DXP FT synthase)" FT /tb_orthologue="dxs" FT misc_feature 1201293..1201352 FT /colour=8 FT /note="PS00801 Transketolase signature 1" FT misc_feature 1202472..1202522 FT /colour=8 FT /note="PS00802 Transketolase signature 2" FT CDS 1203549..1205226 FT /class="I.B.7" FT /colour=0 FT /gene="ML1039" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3727 (Best blastx score 386)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT stem_loop 1204301..1204350 FT /note="possible stem loop structure with 100% identity FT over 48 bases and a loop of 2 bases" FT CDS complement(1205247..1206536) FT /class="V" FT /colour=10 FT /gene="ML1040" FT /note="Identical to the previously sequenced Mycobacterium FT leprae hypothetical protein TR:Q50004 (EMBL:U15181) (429 FT aa); Fasta score E(): 0, 100.0% identity in 429 aa FT overlap. Also highly similar to Mycobacterium tuberculosis FT hypothetical protein Rv2681 TR:O07183 (EMBL:Z96072) (438 FT aa); Fasta score E(): 0, 77.4% identity in 416 aa overlap. FT The predicted product of this CDS is also weakly similar FT to several nucleases e.g. Escherichia coli SW:RND_ECOLI FT (P09155) (375 aa); Fasta score E(): 2.4e-08, 26.7% FT identity in 371 aa overlap ribonuclease D (EC 3.1.26.3). FT Contains Pfam match to entry PF01612 3_5_exonuclease, FT 3'-5' exonuclease." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv2681" FT misc_feature complement(1205940..1206401) FT /colour=0 FT /note="Pfam match to entry PF01612 3_5_exonuclease, 3'-5' FT exonuclease, score 175.90, E-value 6.6e-49" FT CDS complement(1206538..1207128) FT /class="V" FT /colour=10 FT /gene="ML1041" FT /note="Identical to the previously sequenced Mycobacterium FT leprae hypothetical protein TR:Q50005 (EMBL:U15181) (196 FT aa); Fasta score E(): 0, 99.5% identity in 196 aa overlap. FT Also highly similar to hypothetical proteins from FT Mycobacterium tuberculosis Rv2680 TR:O86317 (EMBL:Z96072) FT (210 aa); Fasta score E(): 0, 83.9% identity in 193 aa FT overlap and Streptomyces coelicolor TR:O69860 FT (EMBL:AL023702) (238 aa); Fasta score E(): 2.9e-25, 43.5% FT identity in 186 aa overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv2680" FT RBS complement(1207130..1207135) FT CDS complement(1207289..1208087) FT /class="I.A.3" FT /colour=0 FT /gene="ML1042" FT /note="Possible pseudogene of M. tuberculosis orthologue FT echA15 (Best blastx score 306)" FT /product="enoyl-CoA hydratase/isomerase superfamily FT (pseudogene)" FT /pseudo FT CDS 1208217..1209290 FT /class="I.G.12" FT /colour=7 FT /gene="hemE" FT /gene="ML1043" FT /note="Identical to the previously sequenced Mycobacterium FT leprae uroporphyrinogen decarboxylase (EC 4.1.1.37) FT SW:DCUP_MYCLE (P46809) (357 aa); Fasta score E(): 0, FT 100.0% identity in 357 aa overlap. Also highly similar to FT many others including: Mycobacterium tuberculosis FT SW:DCUP_MYCTU (O53231) (357 aa); Fasta score E(): 0, 83.8% FT identity in 357 aa overlap and Bacillus subtilis FT SW:DCUP_BACSU (P32395) (353 aa); Fasta score E(): 0, 43.5% FT identity in 354 aa overlap. Contains Pfam match to entry FT PF01208 URO-D, Uroporphyrinogen decarboxylase (URO-D). FT Contains PS00907 Uroporphyrinogen decarboxylase signature FT 2. Contains PS00906 Uroporphyrinogen decarboxylase FT signature 1." FT /product="uroporphyrinogen decarboxylase" FT /tb_orthologue="hemE" FT misc_feature 1208223..1209287 FT /colour=0 FT /note="Pfam match to entry PF01208 URO-D, Uroporphyrinogen FT decarboxylase (URO-D), score 824.40, E-value 4e-244" FT misc_feature 1208289..1208318 FT /colour=8 FT /note="PS00906 Uroporphyrinogen decarboxylase signature 1" FT misc_feature 1208643..1208690 FT /colour=8 FT /note="PS00907 Uroporphyrinogen decarboxylase signature 2" FT CDS 1209287..1210642 FT /class="I.G.12" FT /colour=7 FT /gene="hemY'" FT /gene="ML1044" FT /note="Identical to the previously sequenced Mycobacterium FT leprae protoporphyrinogen oxidase (EC 1.3.3.4) FT SW:PPOX_MYCLE (Q50008) (451 aa); Fasta score E(): 0, FT 100.0% identity in 451 aa overlap. Also highly similar to FT many others including: Mycobacterium tuberculosis FT SW:PPOX_MYCTU (O53230) (426 aa); Fasta score E(): 0, 75.8% FT identity in 429 aa overlap and Bacillus subtilis FT SW:PPOX_BACSU (P32397) (470 aa); Fasta score E(): 2.2e-13, FT 27.1% identity in 458 aa overlap. Contains Pfam match to FT entry PF01208 URO-D, Uroporphyrinogen decarboxylase FT (URO-D)." FT /product="protoporphyrinogen oxidase" FT /tb_orthologue="hemY'" FT CDS 1210648..1211343 FT /class="V" FT /colour=10 FT /gene="ML1045" FT /note="Identical, apart from N-terminus, to the previously FT sequenced Mycobacterium leprae hypothetical protein FT TR:Q50009 (EMBL:U15181) (297 aa); Fasta score E(): 0, FT 100.0% identity in 231 aa overlap. Also highly similar to FT proteins of undefined function from Mycobacterium FT tuberculosis Rv2676c TR:P71973 (EMBL:Z80225) (231 aa); FT Fasta score E(): 0, 87.4% identity in 231 aa overlap and FT Streptomyces coelicolor TR:O69830 (EMBL:AL023517) (243 FT aa); Fasta score E(): 0, 61.3% identity in 222 aa FT overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv2676c" FT CDS 1211366..1212072 FT /class="IV.H" FT /colour=0 FT /gene="ML1046" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2675c (Best blastx score 246)" FT /product="putative methyltransferase (pseudogene)" FT /pseudo FT repeat_unit 1212137..1214522 FT CDS complement(1212194..1212938) FT /class="V" FT /colour=0 FT /gene="ML1047" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3714c (Best blastx score 211)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(1214207..1215448) FT /class="IV.B.1.c" FT /colour=0 FT /gene="ML1048" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv2885c (Best blastx score 388)" FT /product="transposase (pseudogene)" FT /pseudo FT CDS complement(1215472..1216054) FT /class="IV.B.1.c" FT /colour=0 FT /gene="ML1049" FT /note="Possible pseudogene of M. tuberculosis orthologue FT resolvase Rv0605 (Best blastx score 351)" FT /product="resolvase (pseudogene)" FT /pseudo FT CDS 1216310..1217032 FT /class="V" FT /colour=0 FT /gene="ML1050" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1191 (Best blastx score 177)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS 1217109..1218539 FT /class="I.A.3" FT /colour=1 FT /gene="xclC" FT /gene="ML1051" FT /note="Identical to the previously sequenced Mycobacterium FT leprae XclC TR:Q50017 (EMBL:U15181) (476 aa); Fasta score FT E(): 0, 99.8% identity in 476 aa overlap. Also highly FT similar to many others including: Mycobacterium FT tuberculosis acyl-CoA synthase Rv1193 fadD36 TR:O05295 FT (EMBL:Z93777) (473 aa); Fasta score E(): 0, 81.3% identity FT in 476 aa overlap and Escherichia coli FT long-chain-fatty-acid--coa ligase (EC 6.2.1.3) FT SW:LCFA_ECOLI (P29212) (561 aa); Fasta score E(): 1.3e-28, FT 31.7% identity in 378 aa overlap. Contains Pfam match to FT entry PF00501 AMP-binding, AMP-binding enzyme. Contains FT PS00455 Putative AMP-binding domain signature. Contains FT PS00038 Myc-type, 'helix-loop-helix' dimerization domain FT signature." FT /product="acyl-CoA synthase" FT /tb_orthologue="fadD36" FT misc_feature 1217190..1217237 FT /colour=8 FT /note="PS00038 Myc-type, 'helix-loop-helix' dimerization FT domain signature" FT misc_feature 1217190..1218335 FT /colour=0 FT /note="Pfam match to entry PF00501 AMP-binding, FT AMP-binding enzyme, score 65.80, E-value 4.4e-19" FT misc_feature 1217517..1217552 FT /colour=8 FT /note="PS00455 Putative AMP-binding domain signature" FT CDS complement(1218562..1219827) FT /class="V" FT /colour=10 FT /gene="ML1052" FT /note="Previously sequenced Mycobacterium leprae FT hypothetical protein TR:Q50018 (EMBL:U15181) (517 aa); FT Fasta score E(): 0, 100.0% identity in 421 aa overlap. FT Also highly similar to several hypothetical proteins from FT Mycobacterium tuberculosis e.g. Rv1194c TR:O05296 FT (EMBL:Z93777) (421 aa); Fasta score E(): 0, 70.1% identity FT in 421 aa overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv1453" FT /tb_orthologue="Rv2370c" FT repeat_unit 1220160..1222101 FT RBS 1220362..1220367 FT CDS 1220374..1220673 FT /class="V" FT /colour=10 FT /gene="ML1053" FT /note="Identical to the previously sequenced Mycobacterium FT leprae hypothetical protein TR:Q49943 (EMBL:U15180) (99 FT aa); Fasta score E(): 0, 100.0% identity in 99 aa overlap. FT Also highly similar to the whole or just the N-terminus of FT many Mycobacterium tuberculosis PE-family proteins e.g. FT TR:O53938 (EMBL:AL022021) (99 aa); Fasta score E(): 2e-11, FT 46.7% identity in 92 aa overlap and TR:P71664 FT (EMBL:Z80108) (576 aa); Fasta score E(): 3.2e-10, 44.6% FT identity in 92 aa overlap." FT /product="conserved hypothetical protein" FT CDS 1220730..1221367 FT /class="V" FT /colour=0 FT /gene="ML1054" FT /note="Similar to Mycobacterium leprae PPE-family protein FT TR:Q9Z5K0." FT /product="possible PPE-family protein (pseudogene)" FT /pseudo FT CDS 1221433..1221735 FT /class="V" FT /colour=10 FT /gene="ML1055" FT /note="Identical to the previously sequenced Mycobacterium FT leprae hypothetical protein TR:Q49945 (EMBL:U15180) (100 FT aa); Fasta score E(): 0, 100.0% identity in 100 aa FT overlap. Also similar to another Mycobacterium leprae FT hypothetical protein TR:Q9S382 (EMBL:AL049191) (94 aa); FT Fasta score E(): 0, 100.0% identity in 94 aa overlap and FT several proteins of undefined function from Mycobacterium FT tuberculosis e.g. TR:O05299 (EMBL:Z93777) (98 aa); Fasta FT score E(): 1.7e-20, 58.3% identity in 96 aa overlap." FT /product="conserved hypothetical protein" FT RBS 1221772..1221776 FT CDS 1221787..1222074 FT /class="V" FT /colour=10 FT /gene="ML1056" FT /note="Identical to the previously sequenced Mycobacterium FT leprae hypothetical protein TR:Q49946 (EMBL:U15180) (95 FT aa); Fasta score E(): 0, 100.0% identity in 95 aa overlap. FT Also highly similar to several proteins of undefined FT function from Mycobacterium tuberculosis e.g. Rv1793 FT TR:O53942 (EMBL:AL022021) (94 aa); Fasta score E(): FT 4.3e-23, 65.2% identity in 92 aa overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv2346c" FT CDS 1222249..1222602 FT /class="VI" FT /colour=8 FT /gene="ML1057" FT /ml_unique FT /note="Identical to the previously sequenced Mycobacterium FT leprae hypothetical protein TR:Q49947 (EMBL:U15180) (117 FT aa); Fasta score E(): 0, 99.1% identity in 117 aa FT overlap." FT /product="hypothetical protein" FT CDS complement(1222693..1223646) FT /class="IV.H" FT /colour=7 FT /gene="ML1058" FT /note="Identical to the previously sequenced Mycobacterium FT leprae hypothetical protein TR:Q49948 (EMBL:U15180) (317 FT aa); Fasta score E(): 0, 99.7% identity in 317 aa overlap FT and highly similar to Mycobacterium tuberculosis TR:O05302 FT (EMBL:Z93777) (317 aa); Fasta score E(): 0, 84.9% identity FT in 317 aa overlap. The C-terminus of this protein is also FT similar to many different transferases e.g. Haemophilus FT influenzae SW:DAPD_HAEIN (P45284) (275 aa); Fasta score FT E(): 0.00048, 26.7% identity in 131 aa overlap FT 2,3,4,5-tetrahydropyridine-2-carboxylate FT N-succinyltransferase (EC 2.3.1.117)." FT /product="possible tranferase" FT /tb_orthologue="Rv1201c" FT CDS 1223700..1224764 FT /class="I.D.2" FT /colour=7 FT /gene="dapE" FT /gene="ML1059" FT /note="Identical to the previously sequenced Mycobacterium FT leprae TR:Q49949 (EMBL:U15180) (400 aa); Fasta score E(): FT 0, 100.0% identity in 354 aa overlap. Also highly similar FT to succinyl-diaminopimelate desuccinylases from FT Mycobacterium tuberculosis Rv1202 TR:O05303 (EMBL:Z93777) FT (354 aa); Fasta score E(): 0, 86.7% identity in 354 aa FT overlap and Corynebacterium glutamicum SW:DAPE_CORG FT (Q59284) (369 aa); Fasta score E(): 0, 56.3% identity in FT 359 aa overlapL. Contains Pfam match to entry PF01546 FT Peptidase_M20, Peptidase family M20/M25/M40. Contains FT PS00758 ArgE / dapE / ACY1 / CPG2 / yscS family signature FT 1." FT /product="succinyl-diaminopimelate desuccinylase" FT /tb_orthologue="dapE" FT misc_feature 1223733..1224617 FT /colour=0 FT /note="Pfam match to entry PF01546 Peptidase_M20, FT Peptidase family M20/M25/M40, score 208.80, E-value 6e-59" FT misc_feature 1223889..1223918 FT /colour=8 FT /note="PS00758 ArgE / dapE / ACY1 / CPG2 / yscS family FT signature 1" FT CDS complement(1224793..1226253) FT /class="V" FT /colour=0 FT /gene="ML1060" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1204c (Best blastx score 256)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS 1226442..1227005 FT /class="V" FT /colour=10 FT /gene="ML1061" FT /note="Identical to the previously sequenced Mycobacterium FT leprae hypothetical protein TR:Q49952 (EMBL:U15180) (187 FT aa); Fasta score E(): 0, 100.0% identity in 187 aa FT overlap. Also highly similar to Mycobacterium tuberculosis FT TR:O05306 (EMBL:Z93777) (187 aa); Fasta score E(): 0, FT 72.4% identity in 174 aa overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv1205" FT CDS 1227056..1228573 FT /class="I.A.3" FT /colour=0 FT /gene="fadD6" FT /gene="ML1062" FT /note="Possible pseudogene of M. tuberculosis orthologue FT fadD6 (Best blastx score 281)" FT /product="acyl CoA-ligase (pseudogene)" FT /pseudo FT CDS 1228776..1229651 FT /class="I.G.2" FT /colour=7 FT /gene="folP" FT /gene="ML1063" FT /note="Identical to the previously sequenced Mycobacterium FT leprae dihydropteroate synthase (EC 2.5.1.15) FT SWDHPS_MYCLE:. Also highly similar to dihydropteroate FT synthases from Mycobacterium tuberculosis Rv1207 FT SW:DHP2_MYCTU (O05308) (318 aa); Fasta score E(): 0, 86.2% FT identity in 290 aa overlap and Escherichia coli FT SW:DHPS_ECOLI (P26282) (282 aa); Fasta score E(): 7.2e-24, FT 34.4% identity in 270 aa overlap. Contains Pfam match to FT entry PF00809 DHPS, Dihydropteroate synthase. Contains FT PS00793 Dihydropteroate synthase signature 2. Contains FT PS00792 Dihydropteroate synthase signature 1." FT /note="Similar to ML0224" FT /product="dihydropteroate synthase" FT /tb_orthologue="folP2" FT misc_feature 1228824..1228871 FT /colour=8 FT /note="PS00792 Dihydropteroate synthase signature 1" FT misc_feature 1228827..1229603 FT /colour=0 FT /note="Pfam match to entry PF00809 DHPS, Dihydropteroate FT synthase, score 457.60, E-value 1e-133" FT misc_feature 1228926..1228967 FT /colour=8 FT /note="PS00793 Dihydropteroate synthase signature 2" FT CDS 1229648..1230628 FT /class="V" FT /colour=10 FT /gene="ML1064" FT /note="Identical to the previously sequenced Mycobacterium FT leprae hypothetical protein TR:Q49955 (EMBL:U15180) (318 FT aa); Fasta score E(): 0, 100.0% identity in 317 aa FT overlap. Also highly similar to Mycobacterium tuberculosis FT hypothetical protein Rv1208 TR:O05309 (EMBL:Z93777) (324 FT aa); Fasta score E(): 0, 79.8% identity in 327 aa FT overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv1208" FT CDS 1230644..1230988 FT /class="V" FT /colour=10 FT /gene="ML1065" FT /note="Identical to the previously sequenced Mycobacterium FT leprae hypothetical protein TR:Q49955. Also highly similar FT to Mycobacterium tuberculosis hypothetical protein Rv1209 FT TR:O05310 (EMBL:Z93777) (122 aa); Fasta score E(): FT 1.3e-27, 78.6% identity in 112 aa overlap. Note the FT N-terminus of this protein is rich in the amino acid FT valine producing a possible membrane spanning hydrophobic FT domain." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv1209" FT CDS 1230985..1231563 FT /class="II.A.5" FT /colour=2 FT /gene="tagA" FT /gene="ML1066" FT /note="Identical to the previously sequenced Mycobacterium FT leprae TR:Q49957 (EMBL:U15180) (192 aa); Fasta score E(): FT 0, 99.5% identity in 192 aa overlap. Also highly similar FT to DNA-3-methyladenine glycosidases from Mycobacterium FT tuberculosis Rv1210 TR:O05311 (EMBL:Z93777) (204 aa); FT Fasta score E(): 0, 80.2% identity in 192 aa overlap and FT Escherichia coli SW:3MG1_ECOLI (P05100) (187 aa); Fasta FT score E(): 1.8e-27, 43.8% identity in 185 aa overlap." FT /product="DNA-3-methyladenine glycosidase I" FT /tb_orthologue="tagA" FT RBS 1231769..1231772 FT CDS 1231777..1232004 FT /class="V" FT /colour=10 FT /gene="ML1067" FT /note="Identical to the previously sequenced Mycobacterium FT leprae hypothetical protein TR:Q49958 (EMBL:U15180) (75 FT aa); Fasta score E(): 1.9e-29, 100.0% identity in 75 aa FT overlap. Also highly similar to Mycobacterium tuberculosis FT hypothetical protein Rv1211 TR:O05312 (EMBL:Z93777) (75 FT aa); Fasta score E(): 1.6e-26, 90.7% identity in 75 aa FT overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv1211" FT CDS complement(1232039..1233169) FT /class="V" FT /colour=0 FT /gene="ML1068" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1212c (Best blastx score 834)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS 1233349..1234563 FT /class="I.C.1" FT /colour=7 FT /gene="glgC" FT /gene="ML1069" FT /note="Identical to the previously sequenced Mycobacterium FT leprae GlgC TR:Q49961 (EMBL:U15180) (419 aa); Fasta score FT E(): 0, 100.0% identity in 404 aa overlap. Also highly FT similar to many glucose-1-phosphate adenylyltransferases FT involved in glycogen biosynthesis e.g. from Mycobacterium FT tuberculosis Rv1213 SW:GLGC_MYCTU (O05314) (404 aa); Fasta FT score E(): 0, 92.6% identity in 404 aa overlap and FT Escherichia coli SW:GLGC_ECOLI (P00584) (430 aa); Fasta FT score E(): 0, 39.6% identity in 407 aa overlap. Contains FT Pfam match to entry PF00483 NTP_transferase, Nucleotidyl FT transferase. Contains PS00809 ADP-glucose FT pyrophosphorylase signature 2. Contains PS00810 FT ADP-glucose pyrophosphorylase signature 3. Contains FT PS00808 ADP-glucose pyrophosphorylase signature 1." FT /product="probable glucose-1-phosphate FT adenylyltransferase" FT /tb_orthologue="glgC" FT misc_feature 1233370..1234185 FT /colour=0 FT /note="Pfam match to entry PF00483 NTP_transferase, FT Nucleotidyl transferase, score 324.90, E-value 9.4e-94" FT misc_feature 1233385..1233444 FT /colour=8 FT /note="PS00808 ADP-glucose pyrophosphorylase signature 1" FT misc_feature 1233640..1233666 FT /colour=8 FT /note="PS00809 ADP-glucose pyrophosphorylase signature 2" FT misc_feature 1233934..1233966 FT /colour=8 FT /note="PS00810 ADP-glucose pyrophosphorylase signature 3" FT CDS 1234637..1235290 FT /class="I.J.1" FT /colour=9 FT /gene="ML1070" FT /note="Identical to the previously sequenced Mycobacterium FT leprae TR:Q49962 (EMBL:U15180) (217 aa); Fasta score E(): FT 0, 100.0% identity in 217 aa overlap. Also highly similar FT to many proteins of undefined function e.g. Mycobacterium FT tuberculosis possible regulatory protein Rv3855 TR:P96222 FT (EMBL:Z83864) (216 aa); Fasta score E(): 0, 60.4% identity FT in 202 aa overlap. Contains a probable helix-turn-helix FT motif at aa 49-70 (Score 1321, SD +3.69) Contains Pfam FT match to entry PF00440 tetR, Bacterial regulatory FT proteins, tetR family." FT /note="Similar to ML0064" FT /product="putative TetR-family transcriptional regulator" FT /tb_orthologue="Rv3855" FT misc_feature 1234733..1234873 FT /colour=0 FT /note="Pfam match to entry PF00440 tetR, Bacterial FT regulatory proteins, tetR family, score 66.90, E-value FT 4.2e-16" FT CDS complement(1235298..1235645) FT /class="V" FT /colour=0 FT /gene="ML1071" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1216c (Best blastx score 244)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(1235874..1236692) FT /class="II.C.4" FT /colour=0 FT /gene="ML1072" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1217c (Best blastx score 362)" FT /product="probable integral membrane protein (pseudogene)" FT /pseudo FT CDS complement(1236830..1237668) FT /class="III.A" FT /colour=0 FT /gene="ML1073" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1218c (Best blastx score 278)" FT /product="probable ABC transmembrane transport protein FT (pseudogene)" FT /pseudo FT CDS complement(1237768..1238309) FT /class="I.J.1" FT /colour=0 FT /gene="ML1074" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1219c (Best blastx score 232)" FT /product="putative transcriptional regulator (pseudogene)" FT /pseudo FT CDS complement(1238386..1239060) FT /class="IV.H" FT /colour=7 FT /gene="ML1075" FT /note="Previously sequenced Mycobacterium leprae TR:Q49969 FT (EMBL:U15180) (136 aa); Fasta score E(): 0, 100.0% FT identity in 136 aa overlap. Also highly similar to FT Mycobacterium tuberculosis possible methyltransferase FT Rv1220c TR:O33219 (EMBL:Z98260) (215 aa); Fasta score E(): FT 0, 85.5% identity in 214 aa overlap and Streptomyces FT mycarofaciens O-methyltransferase (EC 2.1.1.-) involved in FT the synthesis of the macrolide antibiotic midecamycin FT SW:MDMC_STRMY (Q00719) (221 aa); Fasta score E(): 2.3e-11, FT 30.5% identity in 223 aa overlap. Contains Pfam match to FT entry PF01596 Methyltransf_3, O-methyltransferase." FT /product="probable O-methyltransferase" FT /tb_orthologue="Rv1220c" FT misc_feature complement(1238389..1238985) FT /colour=0 FT /note="Pfam match to entry PF01596 Methyltransf_3, FT O-methyltransferase, score 16.80, E-value 3e-10" FT CDS 1239340..1240131 FT /class="II.A.7" FT /colour=2 FT /gene="sigE" FT /gene="ML1076" FT /note="Highly similar to many proposed ECF-family FT RNA-polymerase sigma factors including: Mycobacterium FT tuberculosis SigE TR:O06289 (EMBL:U87242) (257 aa); Fasta FT score E(): 0, 82.1% identity in 252 aa overlap, FT Mycobacterium avium SigE TR:O05735 (EMBL:U87308) (251 aa); FT Fasta score E(): 0, 80.6% identity in 247 aa overlap and FT Bacillus subtilis SW:SIGW_BACSU (Q45585) (187 aa); Fasta FT score E(): 2.9e-16, 35.7% identity in 171 aa overlap. FT Contains a probable helix-turn-helix motif at aa 213-234 FT (Score 1147, SD +3.09). Contains Pfam match to entry FT PF00776 Sigma70_ECF, Sigma-70 factor (ECF subfamily)." FT /product="ECF subfamily sigma subunit" FT /tb_orthologue="sigE" FT misc_feature 1239640..1239804 FT /colour=0 FT /note="Pfam match to entry PF00776 Sigma70_ECF, Sigma-70 FT factor (ECF subfamily), score 97.30, E-value 3.1e-25" FT CDS 1240278..1240697 FT /class="V" FT /colour=10 FT /gene="ML1077" FT /note="Highly similar to several proteins of undefined FT function e.g. from Mycobacterium avium TR:O05736 FT (EMBL:U87308) (133 aa); Fasta score E(): 0, 71.7% identity FT in 138 aa overlap, Mycobacterium smegmatis TR:O05768 FT (EMBL:U87307) (145 aa); Fasta score E(): 0, 66.2% identity FT in 139 aa overlap and Mycobacterium tuberculosis Rv1222 FT TR:O06290 (EMBL:U87242) (193 aa); Fasta score E(): 0, FT 70.7% identity in 150 aa overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv1222" FT CDS 1240772..1242373 FT /class="II.B.3" FT /colour=7 FT /gene="htrA" FT /gene="ML1078" FT /note="Identical to the previously sequenced Mycobacterium FT leprae TR:Q49972 (EMBL:U15180) (533 aa); Fasta score E(): FT 0, 99.8% identity in 533 aa overlap. Also highly similar FT to Mycobacterium tuberculosis possible serine protease FT HtrA TR:O06291 (EMBL:Z98260) (549 aa); Fasta score E(): 0, FT 81.8% identity in 533 aa overlap. The C-terminus of this FT CDS is similar to several proven proteases e.g. FT Escherichia coli SW:HTRA_ECOLI (P09376) (474 aa); Fasta FT score E(): 2e-26, 35.3% identity in 283 aa overlap. FT Contains a possible membrane spanning hydrophobic domain. FT Contains Pfam match to entry PF00595 PDZ, PDZ domain (Also FT known as DHR or GLGF).. Contains Pfam match to entry FT PF00089 trypsin, Trypsin. Contains PS00017 ATP/GTP-binding FT site motif A (P-loop)." FT /product="possinble serine protease" FT /tb_orthologue="htrA" FT misc_feature 1241456..1242070 FT /colour=0 FT /note="Pfam match to entry PF00089 trypsin, Trypsin, score FT 95.30, E-value 2.1e-29" FT misc_feature 1241984..1242007 FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT misc_feature 1242077..1242325 FT /colour=0 FT /note="Pfam match to entry PF00595 PDZ, PDZ domain (Also FT known as DHR or GLGF)., score 33.30, E-value 5.5e-06" FT CDS 1242373..1242735 FT /class="II.C.2" FT /colour=3 FT /gene="ML1079" FT /note="Identical to the previously sequenced Mycobacterium FT leprae hypothetical protein TR:Q49973 (EMBL:U15180) (120 FT aa); Fasta score E(): 0, 98.3% identity in 120 aa overlap. FT Also highly similar to Mycobacterium tuberculosis FT hypothetical protein Rv1224 TR:O33220 (EMBL:Z98260) (131 FT aa); Fasta score E(): 0, 74.0% identity in 131 aa overlap. FT Also similar, in parts, to Escherichia coli TatB or MttA2, FT Sec-independent protein translocase, TR:O69415 FT (EMBL:AJ005830) (171 aa); Fasta score E(): 0.00022, 29.8% FT identity in 121 aa overlap. Contains a possible N-terminal FT signal sequence." FT /product="possible secreted protein" FT /tb_orthologue="Rv1224" FT repeat_unit 1242595..1243819 FT CDS complement(1243755..1244906) FT /class="V" FT /colour=10 FT /gene="mrp" FT /gene="ML1080" FT /note="Previously sequenced Mycobacterium leprae FT SW:MRP_MYCLE (P53382) (435 aa); Fasta score E(): 0, 100.0% FT identity in 383 aa overlap. Similar to many other FT MRP-family proteins, the function of which is unknown, FT e.g. Mycobacterium tuberculosis Rv1229c SW:MRP_MYCTU FT (O33225) (390 aa); Fasta score E(): 0, 82.1% identity in FT 374 aa overlap and Escherichia coli SW:MRP_ECOLI (P21590) FT (379 aa); Fasta score E(): 8.3e-30, 33.3% identity in 360 FT aa overlap. Contains Pfam match to entry PF01883 DUF59, FT Domain of unknown function. Contains PS00017 FT ATP/GTP-binding site motif A (P-loop). Contains PS01215 FT Mrp family signature." FT /product="MRP-family ATP-binding protein" FT /tb_orthologue="mrp" FT misc_feature complement(1244190..1244240) FT /colour=8 FT /note="PS01215 Mrp family signature" FT misc_feature complement(1244505..1244528) FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT misc_feature complement(1244610..1244879) FT /colour=0 FT /note="Pfam match to entry PF01883 DUF59, Domain of FT unknown function, score 22.70, E-value 6.2e-06" FT CDS complement(1245006..1245560) FT /class="II.C.4" FT /colour=0 FT /gene="ML1081" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1230c (Best blastx score 149)" FT /product="possible membrane protein (pseudogene)" FT /pseudo FT CDS complement(1245744..1246192) FT /class="V" FT /colour=0 FT /gene="ML1082" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1231c (Best blastx score 179)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(1246217..1246767) FT /class="V" FT /colour=0 FT /gene="ML1083" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1232c (Best blastx score 228)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(1246851..1247465) FT /class="V" FT /colour=0 FT /gene="ML1084" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1233c (Best blastx score 522)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS 1247769..1248296 FT /class="V" FT /colour=0 FT /gene="ML1085" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1234 (Best blastx score 132)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT RBS 1248317..1248324 FT CDS 1248325..1249731 FT /class="II.C.1" FT /colour=3 FT /gene="ML1086" FT /note="Previously sequenced Mycobacterium leprae TR:Q49975 FT (EMBL:U15180) (469 aa); Fasta score E(): 0, 100.0% FT identity in 468 aa overlap. Also similar to many FT solute-binding transport proteins e.g. from Mycobacterium FT tuberculosis Rv1235 TR:O31095 (EMBL:AF028830) (468 aa); FT Fasta score E(): 0, 77.6% identity in 469 aa overlap and FT Escherichia coli SW:MALE_ECOLI (P02928) (396 aa); Fasta FT score E(): 0.00081, 23.4% identity in 295 aa overlap. FT Contains a possible N-terminal signal sequence and an FT appropriately positioned Prokaryotic membrane lipoprotein FT lipid attachment site. Contains Pfam match to entry FT PF01547 SBP_bacterial_1, Bacterial extracellular FT solute-binding protein. Contains PS00013 Prokaryotic FT membrane lipoprotein lipid attachment site." FT /product="probable solute-binding transport lipoprotein" FT /tb_orthologue="lpqY" FT misc_feature 1248370..1248402 FT /colour=8 FT /note="PS00013 Prokaryotic membrane lipoprotein lipid FT attachment site" FT misc_feature 1248742..1249596 FT /colour=0 FT /note="Pfam match to entry PF01547 SBP_bacterial_1, FT Bacterial extracellular solute-binding protein, score FT 193.60, E-value 3e-54" FT CDS 1249760..1250674 FT /class="III.A.3" FT /colour=3 FT /gene="ML1087" FT /note="Previously sequenced Mycobacterium leprae MalF FT TR:Q49976 (EMBL:U15180) (310 aa); Fasta score E(): 0, FT 100.0% identity in 304 aa overlap. Also highly similar to FT many putative membrane transport proteins including: FT Mycobacterium tuberculosis probable sugar transport FT protein Rv1236 TR:O50452 (EMBL:AL021006) (307 aa); Fasta FT score E(): 0, 81.8% identity in 292 aa overlap and FT Thermococcus litoralis inner membrane protein MalF FT TR:O51924 (EMBL:AF012836) (300 aa); Fasta score E(): 0, FT 39.1% identity in 279 aa overlap. Contains multiple FT possible membrane spanning hydrophobic domains. Contains FT Pfam match to entry PF00528 BPD_transp, FT Binding-protein-dependent transport systems inner membrane FT component." FT /product="probable ABC-transport protein, inner membrane FT component" FT /tb_orthologue="sugA" FT misc_feature 1250318..1250545 FT /colour=0 FT /note="Pfam match to entry PF00528 BPD_transp, FT Binding-protein-dependent transport systems inner membrane FT component, score 38.40, E-value 1.6e-07" FT RBS 1250651..1250657 FT CDS 1250664..1251527 FT /class="III.A.3" FT /colour=3 FT /gene="ML1088" FT /note="Previously sequenced Mycobacterium leprae MalG FT TR:Q49977 (EMBL:U15180) (296 aa); Fasta score E(): 0, FT 100.0% identity in 287 aa overlap. Also highly similar to FT many putative membrane transport proteins including: FT Mycobacterium tuberculosis probable sugar transport FT protein Rv1237 TR:O50453 (EMBL:AL021006) (274 aa); Fasta FT score E(): 0, 89.4% identity in 274 aa overlap and FT Thermococcus litoralis inner membrane protein MalG FT TR:O51925 (EMBL:AF012836) (278 aa); Fasta score E(): FT 4.1e-31, 35.9% identity in 270 aa overlap. Contains FT multiple possible membrane spanning hydrophobic domains. FT Contains Pfam match to entry PF00528 BPD_transp, FT Binding-protein-dependent transport systems inner membrane FT component." FT /product="probable ABC-transport protein, inner membrane FT component" FT /tb_orthologue="sugB" FT misc_feature 1251168..1251398 FT /colour=0 FT /note="Pfam match to entry PF00528 BPD_transp, FT Binding-protein-dependent transport systems inner membrane FT component, score 59.10, E-value 9.8e-14" FT CDS 1251532..1252710 FT /class="III.A.3" FT /colour=3 FT /gene="ML1089" FT /note="Identical to the previously sequenced Mycobacterium FT leprae TR:Q49978 (EMBL:U15180) (392 aa); Fasta score E(): FT 0, 100.0% identity in 392 aa overlap. Also highly similar FT to many ABC-transport proteins including: Mycobacterium FT tuberculosis Rv1238 TR:O50454 (EMBL:AL021006) (393 aa); FT Fasta score E(): 0, 79.9% identity in 389 aa overlap and FT Agrobacterium radiobacter SW:LACK_AGRRD (Q01937) (363 aa); FT Fasta score E(): 0, 53.1% identity in 339 aa overlap FT lactose transport ATP-binding protein. Contains Pfam match FT to entry PF00005 ABC_tran, ABC transporter. Contains FT PS00017 ATP/GTP-binding site motif A (P-loop). Contains FT PS00211 ABC transporters family signature." FT /note="Similar to ML1424" FT /product="probable ABC-transport protein, ATP-binding FT component" FT /tb_orthologue="sugC" FT misc_feature 1251619..1252164 FT /colour=0 FT /note="Pfam match to entry PF00005 ABC_tran, ABC FT transporter, score 200.60, E-value 2.5e-56" FT misc_feature 1251640..1251663 FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT misc_feature 1251934..1251978 FT /colour=8 FT /note="PS00211 ABC transporters family signature" FT CDS complement(1253339..1254448) FT /class="III.A.2" FT /colour=3 FT /gene="ML1090" FT /note="Previously sequenced Mycobacterium leprae TR:Q49979 FT (EMBL:U15180) (373 aa); Fasta score E(): 0, 100.0% FT identity in 369 aa overlap. Highly similar to FT Mycobacterium tuberculosis putative magnesium and cobalt FT transport protein, CorA (Rv1239c) TR:O50455 FT (EMBL:AL021006) (366 aa); Fasta score E(): 0, 79.4% FT identity in 369 aa overlap. Also weakly similar to FT Haemophilus influenzae SW:CORA_HAEIN (P44998) (315 aa); FT Fasta score E(): 2.8e-07, 24.1% identity in 323 aa FT overlap, CorA transporter. Contains possible membrane FT spanning hydrophobic domains. Contains Pfam match to entry FT PF01544 CorA, CorA-like Mg2+ transporter protein." FT /product="probable membrane transport protein" FT /tb_orthologue="corA" FT misc_feature complement(1253342..1254247) FT /colour=0 FT /note="Pfam match to entry PF01544 CorA, CorA-like Mg2+ FT transporter protein, score 487.20, E-value 1.3e-142" FT RBS 1254658..1254662 FT CDS 1254671..1255660 FT /class="I.B.3" FT /colour=7 FT /gene="mdh" FT /gene="ML1091" FT /note="Identical to the previously sequenced Mycobacterium FT leprae malate dehydrogenase (EC 1.1.1.37) SW:MDH_MYCLE FT (P50917) (329 aa); Fasta score E(): 0, 99.7% identity in FT 329 aa overlap. Also highly similar to many other malate FT dehydrogenases including: Mycobacterium tuberculosis FT Rv1240 SW:MDH_MYCTU (O54592) (329 aa); Fasta score E(): 0, FT 89.1% identity in 329 aa overlap and Thermus aquaticus FT SW:MDH_THEFL (P10584) (327 aa); Fasta score E(): 0, 63.7% FT identity in 325 aa overlap. Contains Pfam match to entry FT PF00056 ldh, lactate/malate dehydrogenase. Contains FT PS00687 Aldehyde dehydrogenases glutamic acid active site. FT Contains PS00068 Malate dehydrogenase active site FT signature." FT /product="malate dehydrogenase" FT /tb_orthologue="mdh" FT misc_feature 1254689..1255642 FT /colour=0 FT /note="Pfam match to entry PF00056 ldh, lactate/malate FT dehydrogenase, score 299.00, E-value 5.6e-86" FT misc_feature 1255136..1255174 FT /colour=8 FT /note="PS00068 Malate dehydrogenase active site signature" FT misc_feature 1255265..1255288 FT /colour=8 FT /note="PS00687 Aldehyde dehydrogenases glutamic acid FT active site" FT CDS complement(1255675..1256444) FT /class="IV.C.1.b" FT /colour=0 FT /gene="ML1092" FT /note="Possible pseudogene of M. tuberculosis orthologue FT PE_PGRS (Best blastx score 184)" FT /product="PE_PGRS-family protein (pseudogene)" FT /pseudo FT CDS 1257729..1258586 FT /class="II.C.1" FT /colour=3 FT /gene="ML1093" FT /note="Identical to the previously sequenced Mycobacterium FT leprae hypothetical protein TR:Q49982 (EMBL:U15180) (227 FT aa); Fasta score E(): 0, 100.0% identity in 227 aa FT overlap. Also highly similar to Mycobacterium tuberculosis FT lipoprotein Rv1244, TR:O50459 (EMBL:AL021006) (286 aa); FT Fasta score E(): 0, 73.5% identity in 287 aa overlap and FT weakly similar to several others e.g. Streptomyces FT coelicolor TR:Q9X832 (EMBL:AL049727) (320 aa); Fasta score FT E(): 2.9, 26.1% identity in 299 aa overlap putative FT secreted substrate-binding protein. Contains a possible FT N-terminal signal sequence and an appropriately positioned FT Prokaryotic membrane lipoprotein lipid attachment site. FT Contains PS00013 Prokaryotic membrane lipoprotein lipid FT attachment site." FT /product="lipoprotein" FT /tb_orthologue="lpqZ" FT misc_feature 1257762..1257794 FT /colour=8 FT /note="PS00013 Prokaryotic membrane lipoprotein lipid FT attachment site" FT CDS complement(1259132..1259965) FT /class="I.B.7" FT /colour=7 FT /gene="ML1094" FT /note="Highly similar to several Mycobacterium FT tuberculosis putative oxidoreductases e.g. Rv1245c FT TR:O50460 (EMBL:AL021006) (276 aa); Fasta score E(): 0, FT 78.3% identity in 277 aa overlap. Also similar to many FT others including: Pseudomonas paucimobilis SW:LINX_PSEPA FT (P50198) (250 aa); Fasta score E(): 9.3e-17, 35.6% FT identity in 194 aa overlap FT 2,5-dichloro-2,5-cyclohexadiene-1,4-diol dehydrogenase. FT Contains Pfam match to entry PF00106 adh_short, short FT chain dehydrogenase. Contains PS00061 Short-chain FT dehydrogenases/reductases family signature." FT /product="short chain alcohol dehydrogenase" FT /tb_orthologue="Rv3085" FT misc_feature complement(1259387..1259947) FT /colour=0 FT /note="Pfam match to entry PF00106 adh_short, short chain FT dehydrogenase, score 203.70, E-value 2.7e-57" FT misc_feature complement(1259456..1259542) FT /colour=8 FT /note="PS00061 Short-chain dehydrogenases/reductases FT family signature" FT RBS complement(1259972..1259975) FT CDS complement(1260028..1263810) FT /class="I.B.3" FT /colour=7 FT /gene="odhA" FT /gene="ML1095" FT /note="This CDS appears to constitute the E1 and E2 FT components of the 2-oxoglutarate dehydrogenase complex. FT The N-terminus of this protein is similar to the FT dihydrolipoamide succinyltransferase component E2 and the FT C-terminus is similar to 2-oxoglutarate dehydrogenase FT components E1 e.g. Escherichia coli SW:ODO2_ECOLI (J01619) FT (179 aa); BlastP Expect 3.9 and Escherichia coli FT SW:ODO1_ECOLI (J01619) (179 aa); BlastP Expect 3.9, FT respectively. The predicted product of this CDS is also FT similar, in its entirety, to Corynebacterium glutamicum FT 2-oxoglutarate dehydrogenase (EC 1.2.4.2) TR:P96746 FT (EMBL:D84102) (1257 aa); Fasta score E(): 0, 58.9% FT identity in 1253 aa overlap and Mycobacterium tuberculosis FT 2-oxoglutarate dehydrogenase Rv1248c TR:O50463 FT (EMBL:AL021006) (1214 aa); Fasta score E(): 0, 89.0% FT identity in 1224 aa overlap. Contains Pfam match to entry FT PF00198 2-oxoacid_dh, 2-oxo acid dehydrogenases FT acyltransferase (catalytic domain). Contains Pfam match to FT entry PF00676 E1_dehydrog, Dehydrogenase E1 component." FT /product="2-oxoglutarate dehydrogenase, E1 and E2 FT components" FT /tb_orthologue="sucA" FT misc_feature complement(1261261..1262262) FT /colour=0 FT /note="Pfam match to entry PF00676 E1_dehydrog, FT Dehydrogenase E1 component, score 169.00, E-value 8.1e-47" FT misc_feature complement(1262704..1263387) FT /colour=0 FT /note="Pfam match to entry PF00198 2-oxoacid_dh, 2-oxo FT acid dehydrogenases acyltransferase (catalytic domain), FT score 10.60, E-value 6.2e-10" FT CDS complement(1263932..1264606) FT /class="II.C.4" FT /colour=3 FT /gene="ML1096" FT /note="Highly similar to Mycobacterium tuberculosis FT Rv1249c TR:O50464 (EMBL:AL021006) (262 aa); Fasta score FT E(): 0, 67.3% identity in 223 aa overlap. Contains FT multiple possible membrane spanning hydrophobic domains." FT /product="putative membrane protein" FT CDS 1265104..1266377 FT /class="III.A.6" FT /colour=0 FT /gene="ML1097" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1250 (Best blastx score 225)" FT /product="probable drug efflux protein (pseudogene)" FT /pseudo FT CDS complement(1266648..1270106) FT /class="V" FT /colour=10 FT /gene="ML1098" FT /note="Highly similar to Mycobacterium tuberculosis FT hypothetical protein Rv1251c TR:O50466 (EMBL:AL021006) FT (1139 aa); Fasta score E(): 0, 75.5% identity in 1141 aa FT overlap. Contains PS00017 ATP/GTP-binding site motif A FT (P-loop)." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv1251c" FT misc_feature complement(1267776..1267799) FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT CDS complement(1270157..1270765) FT /class="II.C.1" FT /colour=3 FT /gene="ML1099" FT /note="Highly similar to Mycobacterium tuberculosis FT putative lipoprotein SW:LPRE_MYCTU (Q11065) (202 aa); FT Fasta score E(): 0, 71.3% identity in 202 aa overlap. FT Contains a possible N-terminal signal sequence and an FT appropriately positioned Prokaryotic membrane lipoprotein FT lipid attachment site. Contains PS00013 Prokaryotic FT membrane lipoprotein lipid attachment site." FT /product="putative lipoprotein" FT /tb_orthologue="lprE" FT misc_feature complement(1270679..1270711) FT /colour=8 FT /note="PS00013 Prokaryotic membrane lipoprotein lipid FT attachment site" FT RBS complement(1270769..1270775) FT CDS 1271263..1272632 FT /class="II.A.7" FT /colour=0 FT /gene="ML1100" FT /note="Possible pseudogene of M. tuberculosis orthologue FT deaD (Best blastx score 769)" FT /product="possible ATP-dependent DNA/RNA helicase FT (pseudogene)" FT /pseudo FT CDS 1272632..1273771 FT /class="IV.H" FT /colour=7 FT /gene="ML1101" FT /note="Similar to several acyltransferases including: FT Streptomyces thermotolerans macrolide antibiotics FT 3-O-acyltransferase TR:Q56074 (EMBLD30759:) (389 aa); FT Fasta score E(): 1.3e-14, 29.4% identity in 377 aa overlap FT and Mycobacterium tuberculosis probable acyltransferase FT Rv1254 TR:Q11064 (EMBL:Z77137) (383 aa); Fasta score E(): FT 0, 79.6% identity in 378 aa overlap. Contains multiple FT possible membrane spanning hydrophobic domains." FT /product="possible acyltransferase" FT /tb_orthologue="Rv1254" FT CDS complement(1275354..1276450) FT /class="IV.F" FT /colour=0 FT /gene="ML1102" FT /note="Possible pseudogene of M. tuberculosis orthologue FT probable cytochrome P-450 Rv1256c (Best blastx score 356)" FT /product="possible cytochrome P-450 (pseudogene)" FT /pseudo FT CDS complement(1276458..1277819) FT /class="I.B.7" FT /colour=7 FT /gene="ML1103" FT /note="Similar to many putative oxidoreductase subunits FT including: Escherichia coli glycolate oxidase subunit GlcD FT SW:GLCD_ECOLI (P52075) (499 aa); Fasta score E(): 0, 38.8% FT identity in 456 aa overlap and Mycobacterium tuberculosis FT probable oxidoreductase Rv1257c TR:Q11061 (EMBL:Z77137) FT (455 aa); Fasta score E(): 0, 85.8% identity in 451 aa FT overlap. Contains Pfam match to entry PF01565 FT FAD_binding_4, FAD binding domain." FT /product="possible oxidoreductase subunit" FT /tb_orthologue="Rv1257c" FT misc_feature complement(1277205..1277807) FT /colour=0 FT /note="Pfam match to entry PF01565 FAD_binding_4, FAD FT binding domain, score 321.80, E-value 8.1e-93" FT CDS complement(1277824..1278919) FT /class="III.A.6" FT /colour=0 FT /gene="ML1104" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1258c (Best blastx score 234)" FT /product="probable multidrug resistance pump (pseudogene)" FT /pseudo FT CDS 1279262..1279951 FT /class="V" FT /colour=10 FT /gene="ML1105" FT /note="Similar to many proteins of undefined function FT including: Mycobacterium tuberculosis Rv1259 SW:YC59_MYCTU FT (Q11059) (299 aa); Fasta score E(): 0, 85.5% identity in FT 228 aa overlap and Streptomyces coelicolor TR:Q9S2L3 FT (EMBL:AL109732) (237 aa); Fasta score E(): 0, 54.5% FT identity in 231 aa overlap." FT /product="conserved hypothetical protein." FT stem_loop 1280327..1280369 FT /colour=2 FT /note="possible stem loop structure with 100% matches over FT 21 bases and a 1 nt loop." FT CDS 1280426..1280767 FT /class="VI" FT /colour=8 FT /gene="ML1106" FT /ml_unique FT /note="unknown function." FT /product="hypothetical protein" FT CDS complement(1280738..1281174) FT /class="V" FT /colour=0 FT /gene="ML1107" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1262c (Best blastx score 270)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(1281251..1282166) FT /class="IV.H" FT /colour=0 FT /gene="ML1108" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv0075 (Best blastx score 290)" FT /product="probable aminotransferase (pseudogene)" FT /pseudo FT CDS 1282281..1283491 FT /class="IV.I" FT /colour=0 FT /gene="ML1109" FT /note="Possible pseudogene of M. tuberculosis orthologue FT amiB2 (Best blastx score 454)" FT /product="putative amidase AMI2_MYCTU Q11056 (pseudogene)" FT /pseudo FT CDS 1284313..1285503 FT /class="V" FT /colour=0 FT /gene="ML1110" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv0074 (Best blastx score 257)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS 1285578..1286609 FT /class="IV.J" FT /colour=0 FT /gene="ML1111" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1264 (Best blastx score 409)" FT /product="similar to adenylate cyclases (pseudogene)" FT /pseudo FT CDS 1287170..1287430 FT /class="V" FT /colour=0 FT /gene="ML1112" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1265 (Best blastx score 242)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(1287737..1289626) FT /class="II.C.4" FT /colour=3 FT /gene="ML1113" FT /note="Highly similar to many proposed ABC transporter FT proteins including: Mycobacterium tuberculosis Rv1272c FT SW:YC72_MYCTU (Q11047) (631 aa); Fasta score E(): 0, 75.4% FT identity in 623 aa overlap and Bacillus subtilis FT hypothetical ABC transporter ATP-binding protein FT SW:YFIC_BACSU (P54719) (604 aa); Fasta score E(): 0, 43.2% FT identity in 532 aa overlap. Contains possible membrane FT spanning hydrophobic domains. Contains Pfam match to entry FT PF00664 ABC_membrane, ABC transporter transmembrane FT region. Contains Pfam match to entry PF00005 ABC_tran, ABC FT transporter. Contains PS00017 ATP/GTP-binding site motif A FT (P-loop). Contains PS00211 ABC transporters family FT signature." FT /note="Similar to the adjoining CDS Rv1244" FT /product="probable ABC transporter, ATP-binding component" FT /tb_orthologue="Rv1272c" FT misc_feature complement(1287812..1288366) FT /colour=0 FT /note="Pfam match to entry PF00005 ABC_tran, ABC FT transporter, score 195.20, E-value 1e-54" FT misc_feature complement(1287989..1288033) FT /colour=8 FT /note="PS00211 ABC transporters family signature" FT misc_feature complement(1288322..1288345) FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT misc_feature complement(1288595..1289533) FT /colour=0 FT /note="Pfam match to entry PF00664 ABC_membrane, ABC FT transporter transmembrane region., score 192.50, E-value FT 6.6e-54" FT CDS complement(1289623..1291377) FT /class="II.C.4" FT /colour=3 FT /gene="ML1114" FT /note="Highly similar to many proposed ABC transporter FT proteins including: Mycobacterium tuberculosis Rv1273c FT SW:YC73_MYCTU (Q11046) (582 aa); Fasta score E(): 0, 78.4% FT identity in 583 aa overlap and Bacillus subtilis FT hypothetical ABC transporter ATP-binding protein FT SW:YFIB_BACSU (P54718) (573 aa); Fasta score E(): 0, 34.4% FT identity in 569 aa overlap. Contains possible membrane FT spanning hydrophobic domains. Contains Pfam match to entry FT PF00664 ABC_membrane, ABC transporter transmembrane FT region.. Contains Pfam match to entry PF00005 ABC_tran, FT ABC transporter. Contains PS00017 ATP/GTP-binding site FT motif A (P-loop). Contains PS00211 ABC transporters family FT signature." FT /note="Similar to the adjoining CDS Rv1243" FT /product="probable ABC transporter protein, ATP-binding FT component" FT /tb_orthologue="Rv1273c" FT misc_feature complement(1289731..1290294) FT /colour=0 FT /note="Pfam match to entry PF00005 ABC_tran, ABC FT transporter, score 174.40, E-value 1.8e-48" FT misc_feature complement(1289908..1289952) FT /colour=8 FT /note="PS00211 ABC transporters family signature" FT misc_feature complement(1290250..1290273) FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT misc_feature complement(1290514..1291329) FT /colour=0 FT /note="Pfam match to entry PF00664 ABC_membrane, ABC FT transporter transmembrane region., score 174.40, E-value FT 1.9e-48" FT RBS 1291550..1291553 FT CDS 1291563..1292129 FT /class="II.C.1" FT /colour=3 FT /gene="ML1115" FT /note="Highly similar to Mycobacterium tuberculosis FT possible lipoprotein Rv1274 SW:LPRB_MYCTU (Q11045) (185 FT aa); Fasta score E(): 0, 78.2% identity in 188 aa overlap. FT Contains a possible N-terminal signal sequence and an FT appropriately positioned Prokaryotic membrane lipoprotein FT lipid attachment site. Contains PS00013 Prokaryotic FT membrane lipoprotein lipid attachment site." FT /product="possible lipoprotein" FT /tb_orthologue="lprB" FT misc_feature 1291614..1291646 FT /colour=8 FT /note="PS00013 Prokaryotic membrane lipoprotein lipid FT attachment site" FT RBS 1292131..1292134 FT CDS 1292138..1292701 FT /class="II.C.1" FT /colour=3 FT /gene="ML1116" FT /note="Highly similar to Mycobacterium tuberculosis FT putative lipoprotein LrpC Rv1275 TR:O86337 (EMBL:Z77137) FT (180 aa); Fasta score E(): 0, 82.7% identity in 179 aa FT overlap. Contains a possible N-terminal signal sequence FT and an appropriately positioned Prokaryotic membrane FT lipoprotein lipid attachment site. Contains PS00013 FT Prokaryotic membrane lipoprotein lipid attachment site." FT /product="lipoprotein" FT /tb_orthologue="lprC" FT misc_feature 1292189..1292221 FT /colour=8 FT /note="PS00013 Prokaryotic membrane lipoprotein lipid FT attachment site" FT CDS complement(1292709..1293194) FT /class="V" FT /colour=10 FT /gene="ML1117" FT /note="Highly similar to Mycobacterium tuberculosis FT Rv1276c SW:YC76_MYCTU (Q11043) (158 aa); Fasta score E(): FT 0, 65.4% identity in 162 aa overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv1276c" FT CDS complement(1293281..1294512) FT /class="V" FT /colour=0 FT /gene="ML1118" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1128c (Best blastx score 268)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT RBS 1294754..1294757 FT CDS 1294763..1295914 FT /class="V" FT /colour=10 FT /gene="ML1119" FT /note="Highly similar, in parts,to Mycobacterium FT tuberculosis hypothetical protein Rv1277 TR:Q50699 FT (EMBL:Z77137) (417 aa); Fasta score E(): 0, 82.0% identity FT in 383 aa overlap." FT /product="hypothetical protein" FT /tb_orthologue="Rv1277" FT RBS 1295903..1295906 FT CDS 1295911..1298532 FT /class="V" FT /colour=10 FT /gene="ML1120" FT /note="Highly similar to Mycobacterium tuberculosis FT hypothetical protein Rv1278 SW:YC78_MYCTU (Q11042) (875 FT aa); Fasta score E(): 0, 69.5% identity in 872 aa overlap. FT Contains multiple possible coiled-coils. Contains PS00017 FT ATP/GTP-binding site motif A (P-loop)." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv1278" FT misc_feature 1296001..1296024 FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT CDS complement(1299720..1301393) FT /class="III.A.1" FT /colour=3 FT /gene="ML1121" FT /note="Similar to several including: Mycobacterium FT tuberculosis extracellular solute-binding Rv1280c FT SW:YC80_MYCTU (Q11041) (591 aa); Fasta score E(): 0, 80.3% FT identity in 548 aa overlap and Escherichia coli putative FT binding protein YddS precursor SW:YDDS_ECOLI (P76128) FT (516 aa); Fasta score E(): 2.3e-10, 22.5% identity in 480 FT aa overlap. Contains a possible N-terminal signal sequence FT and an appropriately positioned Prokaryotic membrane FT lipoprotein lipid attachment site. Contains Pfam match to FT entry PF00496 SBP_bac_5, Bacterial extracellular FT solute-binding proteins, family 5. Contains PS00013 FT Prokaryotic membrane lipoprotein lipid attachment site." FT /product="probable extracellular solute-binding dependent FT transport lipoprotein." FT /tb_orthologue="oppA" FT misc_feature complement(1299732..1300688) FT /colour=0 FT /note="Pfam match to entry PF00496 SBP_bac_5, Bacterial FT extracellular solute-binding proteins, family 5, score FT 61.50, E-value 2.3e-16" FT misc_feature complement(1301331..1301363) FT /colour=8 FT /note="PS00013 Prokaryotic membrane lipoprotein lipid FT attachment site" FT CDS complement(1301390..1303219) FT /class="III.A.1" FT /colour=3 FT /gene="ML1122" FT /note="Highly similar to many putative ABC transport FT proteins e.g. Escherichia coli SW:YLIA_ECOLI (P75796) (269 FT aa); BlastP Expect 4.0e-28 Mycobacterium tuberculosis FT Rv1281c SW:YC81_MYCTU (Q11040 (269 aa); BlastP Expect FT 4.0e-28. Note, that the N- and C-termini of this CDS are FT possible duplicates. Contains 2 Pfam matches to entry FT PF00005 ABC_tran, ABC transporter. Contains 2 x PS00017 FT ATP/GTP-binding site motif A (P-loop). Contains 2 x FT PS00211 ABC transporters family signature." FT /product="Probable ABC transport protein, ATP-binding FT component" FT /tb_orthologue="oppD" FT RBS complement(1301397..1301401) FT misc_feature complement(1301492..1302061) FT /colour=0 FT /note="Pfam match to entry PF00005 ABC_tran, ABC FT transporter, score 212.90, E-value 5e-60" FT misc_feature complement(1301678..1301722) FT /colour=8 FT /note="PS00211 ABC transporters family signature" FT misc_feature complement(1302017..1302040) FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT misc_feature complement(1302527..1303120) FT /colour=0 FT /note="Pfam match to entry PF00005 ABC_tran, ABC FT transporter, score 185.80, E-value 6.9e-52" FT misc_feature complement(1302713..1302757) FT /colour=8 FT /note="PS00211 ABC transporters family signature" FT misc_feature complement(1303076..1303099) FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT CDS complement(1303216..1304115) FT /class="III.A.1" FT /colour=3 FT /gene="ML1123" FT /note="Highly similar to many membrane transport proteins FT e.g. Mycobacterium tuberculosis putative peptide transport FT permease Rv1282c SW:YC82_MYCTU (Q10623) (291 aa); Fasta FT score E(): 0, 82.6% identity in 287 aa overlap and FT Escherichia coli SW:OPPC_ECOLI (P77664) (302 aa); Fasta FT score E(): 0, 38.8% identity in 289 aa overlap FT oligopeptide transport system permease protein. Contains FT multiple possible membrane spanning hydrophobic domains. FT Contains Pfam match to entry PF00528 BPD_transp, FT Binding-protein-dependent transport systems inner membrane FT component. Contains PS00402 Binding-protein-dependent FT transport systems inner membrane comp sign.." FT /product="probable binding-protein dependent transport FT protein" FT /tb_orthologue="oppC" FT misc_feature complement(1303348..1303578) FT /colour=0 FT /note="Pfam match to entry PF00528 BPD_transp, FT Binding-protein-dependent transport systems inner membrane FT component, score 29.30, E-value 9.1e-05" FT misc_feature complement(1303489..1303575) FT /colour=8 FT /note="PS00402 Binding-protein-dependent transport systems FT inner membrane comp sign." FT CDS complement(1304112..1305089) FT /class="III.A.1" FT /colour=3 FT /gene="ML1124" FT /note="Highly similar to many membrane transport proteins FT e.g. Mycobacterium tuberculosis putative peptide transport FT permease protein Rv1283c SW:YC83_MYCTU (Q10611) (325 aa); FT Fasta score E(): 0, 83.4% identity in 325 aa overlap and FT Bacillus subtilis oligopeptide transport system permease FT SW:APPB_BACSU (P42062) (317 aa); Fasta score E(): 4.5e-27, FT 34.1% identity in 328 aa overlap. Contains possible FT membrane spanning hydrophobic domains and a possible FT N-terminal signal sequence. Contains Pfam match to entry FT PF00528 BPD_transp, Binding-protein-dependent transport FT systems inner membrane component. Contains PS00402 FT Binding-protein-dependent transport systems inner membrane FT comp sign.." FT /product="probable binding-protein dependent transport FT protein" FT /tb_orthologue="oppB" FT misc_feature complement(1304232..1304447) FT /colour=0 FT /note="Pfam match to entry PF00528 BPD_transp, FT Binding-protein-dependent transport systems inner membrane FT component, score 28.60, E-value 0.00014" FT misc_feature complement(1304358..1304444) FT /colour=8 FT /note="PS00402 Binding-protein-dependent transport systems FT inner membrane comp sign." FT CDS 1305487..1305648 FT /class="V" FT /colour=0 FT /gene="ML1125" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1284 (Best blastx score 190)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(1309374..1309917) FT /class="II.C.1" FT /colour=0 FT /gene="ML1126" FT /note="Possible pseudogene of M. tuberculosis orthologue FT lpqQ (Best blastx score 222)" FT /product="lipoprotein (pseudogene)" FT /pseudo FT tRNA complement(1316300..1316375) FT /colour=4 FT /note="tRNA Arg anticodon CCG, Cove score 91.71" FT RBS 1316558..1316561 FT CDS 1316571..1318223 FT /class="II.A.3" FT /colour=2 FT /gene="argS" FT /gene="ML1127" FT /note="Identical to the previously sequenced Mycobacterium FT leprae arginyl-tRNA synthetase (EC 6.1.1.19) FT (arginine--tRNA ligase) SW:SYR_MYCLE (P45840) (550 aa); FT Fasta score E(): 0, 99.6% identity in 550 aa overlap. Also FT highly similar to many other arginyl-tRNA synthetases FT including: Mycobacterium tuberculosis SW:SYR_MYCTU FT (Q10609) (550 aa); Fasta score E(): 0, 84.9% identity in FT 550 aa overlap and Brevibacterium lactofermentum FT SW:SYR_BRELA (P41253) (550 aa); Fasta score E(): 0, 63.8% FT identity in 550 aa overlap. Contains Pfam match to entry FT PF00750 tRNA-synt_1d, tRNA synthetases class I (R). FT Contains PS00178 Aminoacyl-transfer RNA synthetases FT class-I signature." FT /product="arginyl-tRNA synthase" FT /tb_orthologue="argS" FT misc_feature 1316580..1318220 FT /colour=0 FT /note="Pfam match to entry PF00750 tRNA-synt_1d, tRNA FT synthetases class I (R), score 1082.30, E-value 0" FT misc_feature 1316964..1316993 FT /colour=8 FT /note="PS00178 Aminoacyl-transfer RNA synthetases class-I FT signature" FT CDS 1318220..1319638 FT /class="I.D.2" FT /colour=7 FT /gene="lysA" FT /gene="ML1128" FT /note="Previously sequenced Mycobacterium leprae TR:Q50140 FT (EMBL:U15186) (495 aa); Fasta score E(): 0, 100.0% FT identity in 472 aa overlap. Highly similar to many FT diaminopimelate decarboxylases (EC 4.1.1.20) involved in FT the last step of lysine biosynthesis e.g. Mycobacterium FT tuberculosis, LysA, SW:DCDA_MYCTU (P31848) (447 aa); Fasta FT score E(): 0, 86.8% identity in 446 aa overlap. Contains FT Pfam match to entry PF00750 tRNA-synt_1d, tRNA synthetases FT class I (R). Contains Pfam match to entry PF00278 FT Orn_DAP_Arg_deC, Pyridoxal-dependent decarboxylase. FT Contains PS00878 Orn/DAP/Arg decarboxylases family 2 FT pyridoxal-P attachment site." FT /product="diaminopimelate decarboxylase" FT /tb_orthologue="lysA" FT misc_feature 1318418..1319542 FT /colour=0 FT /note="Pfam match to entry PF00278 Orn_DAP_Arg_deC, FT Pyridoxal-dependent decarboxylase, score 337.70, E-value FT 3.8e-100" FT misc_feature 1318499..1318555 FT /colour=8 FT /note="PS00878 Orn/DAP/Arg decarboxylases family 2 FT pyridoxal-P attachment site" FT RBS 1319632..1319637 FT CDS 1319642..1320967 FT /class="I.D.2" FT /colour=7 FT /gene="hom" FT /gene="ML1129" FT /note="Identical to the previously sequenced Mycobacterium FT leprae homoserine dehydrogenase (EC 1.1.1.3) SW:DHOM_MYCLE FT (P46806) (441 aa); Fasta score E(): 0, 99.8% identity in FT 441 aa overlap. Also highly similar to orthologues from FT Mycobacterium tuberculosis SW:DHOM_MYCTU (Q10601) (441 FT aa); Fasta score E(): 0, 89.5% identity in 438 aa overlap FT and Corynebacterium glutamicum SW:DHOM_CORGL (P08499) (445 FT aa); Fasta score E(): 0, 60.3% identity in 431 aa overlap. FT Contains Pfam match to entry PF00742 Homoserine_dh, FT Homoserine dehydrogenase. Contains Pfam match to entry FT PF01842 ACT, ACT domain. Contains PS01042 Homoserine FT dehydrogenase signature." FT /product="homoserine dehydrogenase" FT /tb_orthologue="thrA" FT misc_feature 1319696..1320619 FT /colour=0 FT /note="Pfam match to entry PF00742 Homoserine_dh, FT Homoserine dehydrogenase, score 554.10, E-value 9e-163" FT misc_feature 1320194..1320262 FT /colour=8 FT /note="PS01042 Homoserine dehydrogenase signature" FT misc_feature 1320704..1320940 FT /colour=0 FT /note="Pfam match to entry PF01842 ACT, ACT domain, score FT 47.40, E-value 3.2e-10" FT CDS 1320964..1322046 FT /class="I.D.2" FT /colour=7 FT /gene="thrC" FT /gene="ML1130" FT /note="Identical to the previously sequenced Mycobacterium FT leprae probable threonine synthase (EC 4.2.99.2) FT SW:THRC_MYCLE (P45837;) (360 aa); Fasta score E(): 0, FT 99.7% identity in 360 aa overlap. Also highly similar to FT many other threonine synthases e.g. Mycobacterium FT tuberculosis SW:THRC_MYCTU (Q10610) (360 aa); Fasta score FT E(): 0, 94.9% identity in 354 aa overlap and FT Brevibacterium lactofermentum SW:THRC_BRELA (P09123) (352 FT aa); Fasta score E(): 0, 57.9% identity in 337 aa overlap. FT Contains Pfam match to entry PF00291 S_T_dehydratase, FT Pyridoxal-phosphate dependent enzyme. Contains PS00165 FT Serine/threonine dehydratases pyridoxal-phosphate FT attachment site." FT /product="threonine synthase" FT /tb_orthologue="thrC" FT misc_feature 1321057..1321947 FT /colour=0 FT /note="Pfam match to entry PF00291 S_T_dehydratase, FT Pyridoxal-phosphate dependent enzyme, score 330.20, FT E-value 2.3e-95" FT misc_feature 1321141..1321182 FT /colour=8 FT /note="PS00165 Serine/threonine dehydratases FT pyridoxal-phosphate attachment site" FT CDS 1322114..1323061 FT /class="I.D.2" FT /colour=7 FT /gene="thrB" FT /gene="ML1131" FT /note="Identical to the previously sequenced Mycobacterium FT leprae homoserine kinase (EC 2.7.1.39) SW:KHSE_MYCLE FT (P45836) (314 aa); Fasta score E(): 0, 99.7% identity in FT 314 aa overlap. Also highly similar to many other FT homoserine kinases including: Mycobacterium tuberculosis FT SW:KHSE_MYCTU (Q10603) (316 aa); Fasta score E(): 0, 82.0% FT identity in 311 aa overlap and Corynebacterium glutamicum FT SW:KHSE_CORGL (P08210) (308 aa); Fasta score E(): 0, 48.7% FT identity in 312 aa overlap. Contains Pfam match to entry FT PF00288 GHMP_kinases, GHMP kinases putative ATP-binding FT proteins. Contains PS00627 GHMP kinases putative FT ATP-binding domain." FT /product="homoserine kinase" FT /tb_orthologue="thrB" FT misc_feature 1322333..1322536 FT /colour=0 FT /note="Pfam match to entry PF00288 GHMP_kinases, GHMP FT kinases putative ATP-binding proteins, score 76.30, FT E-value 3.8e-20" FT misc_feature 1322396..1322431 FT /colour=8 FT /note="PS00627 GHMP kinases putative ATP-binding domain" FT CDS 1323331..1325163 FT /class="II.A.7" FT /colour=2 FT /gene="rho" FT /gene="ML1132" FT /note="Identical to the previously sequenced Mycobacterium FT leprae transcription termination factor Rho SW:RHO_MYCLE FT (P45835) (610 aa); Fasta score E(): 0, 99.8% identity in FT 610 aa overlap. Also highly similar to many other FT transcriptional terminators ihncluding: Mycobacterium FT tuberculosis SW:RHO_MYCTU (Q10607) (602 aa); Fasta score FT E(): 0, 81.5% identity in 616 aa overlap and Micrococcus FT luteus SW:RHO_MICLU (P52154) (690 aa); Fasta score E(): 0, FT 66.0% identity in 467 aa overlap. Contains Pfam match to FT entry PF00006 ATP-synt_ab, ATP synthase alpha/beta FT family." FT /product="transcription termination factor Rho" FT /tb_orthologue="rho" FT misc_feature 1324030..1324977 FT /colour=0 FT /note="Pfam match to entry PF00006 ATP-synt_ab, ATP FT synthase alpha/beta family, score -74.70, E-value 9.9e-06" FT misc_feature 1325474..1325677 FT /colour=0 FT /note="Pfam match to entry PF01197 Ribosomal_L31, FT Ribosomal protein L31, score 160.50, E-value 2.9e-44" FT CDS 1325474..1325728 FT /class="II.A.1" FT /colour=2 FT /gene="rpmE" FT /gene="ML1133" FT /note="Identical to the previously sequenced Mycobacterium FT leprae 50s ribosomal protein L31 SW:RL31_MYCLE (P45834) FT (84 aa); Fasta score E(): 0, 100.0% identity in 84 aa FT overlap, as well as being highly similar to many others FT e.g. Mycobacterium tuberculosis SW:RL31_MYCTU (Q10608) (80 FT aa); Fasta score E(): 5.8e-27, 89.6% identity in 77 aa FT overlap and Escherichia coli SW:RL31_ECOLI (P02432) (70 FT aa); Fasta score E(): 3.4e-11, 53.8% identity in 65 aa FT overlap. Contains Pfam match to entry PF01197 FT Ribosomal_L31, Ribosomal protein L31. Contains PS00190 FT Cytochrome c family heme-binding site signature. Contains FT PS01143 Ribosomal protein L31 signature." FT /product="50S ribosomal protein L31" FT /tb_orthologue="rpmE" FT misc_feature 1325585..1325602 FT /colour=8 FT /note="PS00190 Cytochrome c family heme-binding site FT signature" FT misc_feature 1325597..1325653 FT /colour=8 FT /note="PS01143 Ribosomal protein L31 signature" FT RBS 1325809..1325813 FT CDS 1325819..1326904 FT /class="II.A.6" FT /colour=2 FT /gene="prfA" FT /gene="ML1134" FT /note="Identical to the previously sequenced Mycobacterium FT leprae peptide chain release factor 1 (RF-1), directing FT the termination of translation, SW:RF1_MYCLE (P45833) (357 FT aa); Fasta score E(): 0, 99.7% identity in 357 aa overlap. FT Also highly similar to many other Prokaryotic and FT Eukaryotic chain release factors e.g. from Mycobacterium FT tuberculosis SW:RF1_MYCTU (Q10605) (357 aa); Fasta score FT E(): 0, 89.4% identity in 360 aa overlap and Kluyveromyces FT lactis (Yeast) SW:RF1M_KLULA (P41767) (396 aa); Fasta FT score E(): 0, 39.9% identity in 368 aa overlap. Contains FT Pfam match to entry PF00472 RF-1, Peptidyl-tRNA hydrolase FT domain. Contains PS00745 Prokaryotic-type class I peptide FT chain release factors signature." FT /note="ML0667" FT /product="peptide chain release factor 1" FT /tb_orthologue="prfA" FT misc_feature 1326455..1326796 FT /colour=0 FT /note="Pfam match to entry PF00472 RF-1, Peptidyl-tRNA FT hydrolase domain, score 238.90, E-value 7.4e-68" FT misc_feature 1326515..1326565 FT /colour=8 FT /note="PS00745 Prokaryotic-type class I peptide chain FT release factors signature" FT CDS 1326901..1327767 FT /class="I.G.12" FT /colour=7 FT /gene="ML1135" FT /note="Highly similar to numerous members of the HemK FT family of modification methylases such as Mycobacterium FT tuberculosis, Rv1300, SW:HEMK_MYCTU (Q10602) (325 aa); FT Fasta score E(): 0, 73.9% identity in 284 aa overlap and FT Escherichia coli SW:HEMK_ECOLI (P37186) (277 aa); Fasta FT score E(): 2.4e-17, 32.5% identity in 277 aa overlap." FT /product="possible protoporphyrinogen oxidase" FT RBS 1327800..1327804 FT CDS 1327807..1328469 FT /class="V" FT /colour=10 FT /gene="ML1136" FT /note="Identical to the previously sequenced Mycobacterium FT leprae hypothetical protein SW:YD01_MYCLE (P45831) (220 FT aa); Fasta score E(): 0, 99.5% identity in 220 aa overlap. FT Also highly similar to several other proteins of undefined FT function e.g. Mycobacterium tuberculosis Rv1301 FT SW:YD01_MYCTU (Q10618) (217 aa); Fasta score E(): 0, 83.3% FT identity in 215 aa overlap. Contains Pfam match to entry FT PF01300 Sua5_yciO_yrdC, SUA5/yciO/yrdC family. Contains FT PS01147 SUA5/yciO/yrdC family signature." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv1301" FT misc_feature 1327876..1328406 FT /colour=0 FT /note="Pfam match to entry PF01300 Sua5_yciO_yrdC, FT SUA5/yciO/yrdC family, score 268.20, E-value 1.1e-76" FT misc_feature 1327900..1327938 FT /colour=8 FT /note="PS01147 SUA5/yciO/yrdC family signature" FT CDS 1328500..1329699 FT /class="IV.H" FT /colour=7 FT /gene="ML1137" FT /note="Previously sequenced Mycobacterium leprae FT SW:RFE_MYCLE (P45830) (398 aa); Fasta score E(): 0, 99.7% FT identity in 398 aa overlap. Similar to many including: FT Mycobacterium tuberculosis putative undecaprenyl-phosphate FT alpha-N-acetylglucosaminyltransferase SW:RFE_MYCTU FT (Q10606) (404 aa); Fasta score E(): 0, 89.2% identity in FT 398 aa overlap and Bacillus subtilis FT phospho-N-acetylmuramoyl-pentapeptide-transferase FT SW:MRAY_BACSU (Q03521) (324 aa); Fasta score E(): 1.9e-07, FT 28.6% identity in 297 aa overlap. Contains multiple FT possible membrane spanning hydrophobic domains. Contains FT Pfam match to entry PF00953 Glycos_transf_4, Glycosyl FT transferase." FT /product="possible glycosyltransferase" FT /tb_orthologue="rfe" FT misc_feature 1328827..1329345 FT /colour=0 FT /note="Pfam match to entry PF00953 Glycos_transf_4, FT Glycosyl transferase, score 211.60, E-value 1.2e-59" FT RBS 1329951..1329955 FT CDS 1329962..1330423 FT /class="II.C.4" FT /colour=3 FT /gene="ML1138" FT /note="Identical to the previously sequenced Mycobacterium FT leprae SW:YD03_MYCLE (P53431) (153 aa); Fasta score E(): FT 0, 99.3% identity in 153 aa overlap. Also highly similar FT to Mycobacterium tuberculosis hypothetical protein Rv1303 FT SW:YD03_MYCTU (Q10619) (161 aa); Fasta score E(): 0, 69.8% FT identity in 149 aa overlap. Contains possible membrane FT spanning hydrophobic domains." FT /product="possible integral membrane protein" FT CDS 1330420..1331175 FT /class="I.B.8" FT /colour=7 FT /gene="atpB" FT /gene="ML1139" FT /note="Identical to the previously sequenced Mycobacterium FT leprae ATP synthase A chain (EC 3.6.1.34) SW:ATP6_MYCLE FT (P45829) (251 aa); Fasta score E(): 0, 100.0% identity in FT 251 aa overlap. Also highly similar to many other FT Prokaryotic and Eukaryotic proposed ATPase A chain family FT proteins e.g. Mycobacterium tuberculosis Rv1304 FT SW:ATP6_MYCTU (Q10591) (250 aa); Fasta score E(): 0, 84.0% FT identity in 250 aa overlap and Nicotiana tabacum (Common FT tobacco) SW:ATPI_TOBAC (P06288) (247 aa); Fasta score E(): FT 3.3e-18, 32.5% identity in 246 aa overlap. Contains FT possible membrane spanning hydrophobic domains. Contains FT Pfam match to entry PF00119 ATP-synt_A, ATP synthase A FT chain. Contains PS00449 ATP synthase a subunit signature." FT /product="probable ATP synthase A chain protein" FT /tb_orthologue="atpB" FT misc_feature 1330672..1331145 FT /colour=0 FT /note="Pfam match to entry PF00119 ATP-synt_A, ATP FT synthase A chain, score 210.10, E-value 3.4e-59" FT misc_feature 1330966..1330995 FT /colour=8 FT /note="PS00449 ATP synthase a subunit signature" FT RBS 1331259..1331263 FT CDS 1331271..1331516 FT /class="I.B.8" FT /colour=7 FT /gene="atpE" FT /gene="ML1140" FT /note="Identical to the previously sequenced Mycobacterium FT leprae ATP synthase C chain protein (EC 3.6.1.34) FT SW:ATPL_MYCLE (P45828) (81 aa); Fasta score E(): 1.1e-25, FT 100.0% identity in 81 aa overlap. Also highly similar to FT many other Prokaryotic and Eukaryotic proposed ATPase C FT chain family proteins e.g. Mycobacterium tuberculosis FT Rv1305 SW:ATPL_MYCTU (Q10598) (81 aa); Fasta score E(): FT 6.4e-24, 92.6% identity in 81 aa overlap and Euglena FT gracilis SW:ATPH_EUGGR (P10603) (81 aa); Fasta score E(): FT 4.8e-06, 36.7% identity in 79 aa overlap. Contains FT possible membrane spanning hydrophobic domains. Contains FT Pfam match to entry PF00137 ATP-synt_C, ATP synthase FT subunit C. Contains PS00605 ATP synthase c subunit FT signature." FT /product="probable ATP synthase C chain" FT /tb_orthologue="atpE" FT misc_feature 1331292..1331501 FT /colour=0 FT /note="Pfam match to entry PF00137 ATP-synt_C, ATP FT synthase subunit C, score 93.30, E-value 4.7e-24" FT misc_feature 1331388..1331453 FT /colour=8 FT /note="PS00605 ATP synthase c subunit signature" FT CDS 1331538..1332050 FT /class="I.B.8" FT /colour=7 FT /gene="atpF" FT /gene="ML1141" FT /note="Identical to the previously sequenced Mycobacterium FT leprae ATP synthase B chain (EC 3.6.1.34) SW:ATPF_MYCLE FT (P45827) (170 aa); Fasta score E(): 0, 100.0% identity in FT 170 aa overlap. Also highly similar to many other proposed FT ATPase B chain family proteins e.g. Mycobacterium FT tuberculosis Rv1306 SW:ATPF_MYCTU (Q10596) (171 aa); Fasta FT score E(): 0, 79.5% identity in 171 aa overlap and FT Bacillus PS3 (Thermophilic bacterium PS-3) SW:ATPF_BACP3 FT (P09221) (163 aa); Fasta score E(): 0.00044, 22.5% FT identity in 142 aa overlap. Contains possible membrane FT spanning hydrophobic domains. Contains Pfam match to entry FT PF00430 ATP-synt_B, ATP synthase B/B' CF(0)." FT /product="probable ATP synthase B chain" FT /tb_orthologue="atpF" FT misc_feature 1331622..1332032 FT /colour=0 FT /note="Pfam match to entry PF00430 ATP-synt_B, ATP FT synthase B/B' CF(0), score 137.60, E-value 4.5e-38" FT CDS 1332056..1333396 FT /class="I.B.8" FT /colour=7 FT /gene="atpH" FT /gene="ML1142" FT /note="Previously sequenced Mycobacterium leprae FT SW:ATPD_MYCLE (P53006) (446 aa); Fasta score E(): 0, FT 100.0% identity in 446 aa overlap. The N-terminus of this FT protein shares high sequence identity with many ATP FT synthase B chain proteins e.g. Haemophilus influenzae FT SW:ATPF_HAEIN (P43720) (156 aa); Fasta score E(): 4.8e-05, FT 24.8% identity in 153 aa overlap and the C-terminus shares FT high homology with many ATP synthase [delta] chain FT proteins e.g. Streptomyces lividans SW:ATPD_STRLI (P50008) FT (272 aa); Fasta score E(): 3.4e-20, 33.1% identity in 266 FT aa overlap_ATP. Also highly similar over the entire range FT to Mycobacterium tuberculosis Rv1307 SW:ATPD_MYCTU FT (Q10594) (446 aa); Fasta score E(): 0, 77.8% identity in FT 446 aa overlap. Contains possible coiled-coils and a FT possible N-terminal signal sequence. Contains Pfam match FT to entry PF00213 OSCP, ATP synthase delta (OSCP) subunit. FT Contains Pfam match to entry PF00430 ATP-synt_B, ATP FT synthase B/B' CF(0). Contains PS01094 Uncharacterized FT protein family UPF0076 signature." FT /product="bi-domained ATP synthase B, [Delta] chain FT protein" FT /tb_orthologue="atpH" FT misc_feature 1332062..1332526 FT /colour=0 FT /note="Pfam match to entry PF00430 ATP-synt_B, ATP FT synthase B/B' CF(0), score 58.70, E-value 1.6e-15" FT misc_feature 1332746..1332811 FT /colour=8 FT /note="PS01094 Uncharacterized protein family UPF0076 FT signature" FT misc_feature 1333166..1333360 FT /colour=0 FT /note="Pfam match to entry PF00213 OSCP, ATP synthase FT delta (OSCP) subunit, score 39.40, E-value 1.4e-10" FT CDS 1333458..1335134 FT /class="I.B.8" FT /colour=7 FT /gene="atpA" FT /gene="ML1143" FT /note="Identical to the previously sequenced Mycobacterium FT leprae ATP synthase alpha chain (EC 3.6.1.34) FT SW:ATPA_MYCLE (P45825) (558 aa); Fasta score E(): 0, FT 100.0% identity in 558 aa overlap. Also highly similar FT tomany otherproposed ATPase alpha chain family proteins FT e.g. Mycobacterium tuberculosis Rv1308 TR:ATPA_MYCTU and FT Streptomyces lividans SW:ATPA_STRLI (P50001) (528 aa); FT Fasta score E(): 0, 69.0% identity in 516 aa overlap. FT Contains Pfam match to entry PF00006 ATP-synt_ab, ATP FT synthase alpha/beta family. Contains Pfam match to entry FT PF00422 ATP-synt_A-c, ATP synthase Alpha chain, C FT terminal. Contains PS00017 ATP/GTP-binding site motif A FT (P-loop). Contains PS00152 ATP synthase alpha and beta FT subunits signature." FT /product="ATP synthase [alpha] chain" FT /tb_orthologue="atpA" FT misc_feature 1333545..1334585 FT /colour=0 FT /note="Pfam match to entry PF00006 ATP-synt_ab, ATP FT synthase alpha/beta family, score 570.00, E-value FT 1.5e-167" FT misc_feature 1333971..1333994 FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT misc_feature 1334553..1334582 FT /colour=8 FT /note="PS00152 ATP synthase alpha and beta subunits FT signature" FT misc_feature 1334586..1334990 FT /colour=0 FT /note="Pfam match to entry PF00422 ATP-synt_A-c, ATP FT synthase Alpha chain, C terminal, score 317.90, E-value FT 1.4e-107" FT RBS 1335146..1335151 FT CDS 1335152..1336048 FT /class="I.B.8" FT /colour=7 FT /gene="atpG" FT /gene="ML1144" FT /note="Identical to the previously sequenced Mycobacterium FT leprae ATP synthase gamma chain (EC 3.6.1.34) FT SW:ATPG_MYCLE (P45824) (298 aa); Fasta score E(): 0, FT 100.0% identity in 298 aa overlap. Also highly similar to FT many other proposed ATPase gamma chain family proteins FT e.g. Mycobacterium tuberculosis Rv1309 SW:ATPG_MYCTU FT (Q10597) (305 aa); Fasta score E(): 0, 83.9% identity in FT 305 aa overlap and Streptomyces lividans SW:ATPG_STRLI FT (P50007) (302 aa); Fasta score E(): 0, 48.2% identity in FT 301 aa overlap. Contains Pfam match to entry PF00231 FT ATP-synt, ATP synthase. Contains PS00153 ATP synthase FT gamma subunit signature." FT /product="ATP synthase [gamma] chain" FT /tb_orthologue="atpG" FT misc_feature 1335194..1336033 FT /colour=0 FT /note="Pfam match to entry PF00231 ATP-synt, ATP synthase, FT score 478.40, E-value 5.5e-140" FT misc_feature 1335989..1336030 FT /colour=8 FT /note="PS00153 ATP synthase gamma subunit signature" FT CDS 1336075..1337532 FT /class="I.B.8" FT /colour=7 FT /gene="atpD" FT /gene="ML1145" FT /note="Identical to the previously sequenced Mycobacterium FT leprae ATP synthase beta chain (EC 3.6.1.34) SW:ATPB_MYCLE FT (P45823) (485 aa); Fasta score E(): 0, 100.0% identity in FT 485 aa overlap. Also highly similar to many other proposed FT ATPase beta chain family proteins e.g. Mycobacterium FT tuberculosis Rv1310 SW:ATPB_MYCTU (Q10593) (486 aa); Fasta FT score E(): 0, 92.6% identity in 484 aa overlap and FT Streptomyces lividan SW:ATPB_STRLI (P50004) (477 aa); FT Fasta score E(): 0, 70.3% identity in 478 aa overlap. FT Contains Pfam match to entry PF00006 ATP-synt_ab, ATP FT synthase alpha/beta family. Contains Pfam match to entry FT PF00306 ATP-synt_ab_C, ATP synthase ab C terminal. FT Contains PS00017 ATP/GTP-binding site motif A (P-loop). FT Contains PS00152 ATP synthase alpha and beta subunits FT signature." FT /product="ATP synthase [beta] chain" FT /tb_orthologue="atpD" FT misc_feature 1336147..1337166 FT /colour=0 FT /note="Pfam match to entry PF00006 ATP-synt_ab, ATP FT synthase alpha/beta family, score 526.60, E-value FT 1.8e-154" FT misc_feature 1336582..1336605 FT /colour=8 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)" FT misc_feature 1337134..1337163 FT /colour=8 FT /note="PS00152 ATP synthase alpha and beta subunits FT signature" FT misc_feature 1337230..1337526 FT /colour=0 FT /note="Pfam match to entry PF00306 ATP-synt_ab_C, ATP FT synthase ab C terminal, score 185.20, E-value 1e-51" FT CDS 1337565..1337930 FT /class="I.B.8" FT /colour=7 FT /gene="atpC" FT /gene="ML1146" FT /note="Identical to the previously sequenced Mycobacterium FT leprae ATP synthase epsilon chain (EC 3.6.1.34) FT SW:ATPE_MYCLE (P45822) (124 aa); Fasta score E(): 0, FT 100.0% identity in 121 aa overlap. Also highly similar to FT many other proposed ATPase epsilon chain family proteins FT e.g. Mycobacterium tuberculosis Rv1311 SW:ATPE_MYCTU FT (Q10595) (121 aa); Fasta score E(): 0, 87.6% identity in FT 121 aa overlap and Bacillus firmus SW:ATPE_BACFI (P22480) FT (133 aa); Fasta score E(): 6.3e-09, 41.3% identity in 104 FT aa overlap. Contains Pfam match to entry PF00401 FT ATP-synt_DE, ATP synthase, Delta/Epsilon chain." FT /product="ATP synthase [epsilon] chain" FT /tb_orthologue="atpC" FT misc_feature 1337568..1337906 FT /colour=0 FT /note="Pfam match to entry PF00401 ATP-synt_DE, ATP FT synthase, Delta/Epsilon chain, score 154.00, E-value FT 2.6e-42" FT CDS 1337937..1338380 FT /class="II.C.2" FT /colour=3 FT /gene="ML1147" FT /note="Identical to the previously sequenced Mycobacterium FT leprae hypothetical protein SW:YD12_MYCLE (P53432) (147 FT aa); Fasta score E(): 0, 100.0% identity in 147 aa FT overlap. Also highly similar to Mycobacterium tuberculosis FT hypothetical protein Rv1312 TR:YD12_MYCTU. Contains a FT possible N-terminal signal sequence." FT /product="possible secreted protein" FT /tb_orthologue="Rv1312" FT CDS complement(1338519..1338674) FT /class="VI" FT /colour=8 FT /gene="ML1148" FT /ml_unique FT /note="Identical to the previously sequenced Mycobacterium FT leprae TR:Q50137 (EMBL:U15186) (51 aa); Fasta score E(): FT 4.9e-22, 100.0% identity in 51 aa overlap." FT /product="doubtful CDS" FT CDS complement(1338887..1339462) FT /class="V" FT /colour=10 FT /gene="ML1149" FT /note="Identical to the previously sequenced Mycobacterium FT leprae hypothetical protein SW:YD14_MYCLE (P53523) (191 FT aa); Fasta score E(): 0, 100.0% identity in 191 aa FT overlap. Also similar to many other proteins of undefined FT function e.g. Mycobacterium tuberculosis hypothetical FT protein Rv1314c SW:YD14_MYCTU (Q10622) (193 aa); Fasta FT score E(): 0, 81.2% identity in 191 aa overlap. Contains FT Pfam match to entry PF01923 DUF80, Protein of unknown FT function." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv1314c" FT misc_feature complement(1338920..1339441) FT /colour=0 FT /note="Pfam match to entry PF01923 DUF80, Protein of FT unknown function, score 379.20, E-value 4.3e-110" FT CDS 1339580..1340836 FT /class="II.C.3" FT /colour=3 FT /gene="murA" FT /gene="ML1150" FT /note="Identical to the previously sequenced Mycobacterium FT leprae UDP-N-acetylglucosamine 1-carboxyvinyltransferase FT (EC 2.5.1.7) SW:MURA_MYCLE (P45821) (418 aa); Fasta score FT E(): 0, 99.8% identity in 418 aa overlap representing the FT first step in peptidoglycan biosynthesis. Also highly FT similar to many other UDP-N-acetylglucosamine FT 1-carboxyvinyltransferases e.g. Mycobacterium tuberculosis FT Rv1315 SW:MURA_MYCTU (Q10604) (418 aa); Fasta score E(): FT 0, 95.9% identity in 417 aa overlap and Escherichia coli FT SW:MURA_ECOLI (P2890) (419 aa); Fasta score E(): 0, 45.7% FT identity in 416 aa overlap. Contains Pfam match to entry FT PF00275 EPSP_syntase, EPSP synthase (3-phosphoshikimate FT 1-carboxyvinyltransferase)." FT /product="UDP-N-acetylglucosamine-1-carboxyvinyltransferase" FT /tb_orthologue="murA" FT misc_feature 1339598..1340797 FT /colour=0 FT /note="Pfam match to entry PF00275 EPSP_syntase, EPSP FT synthase (3-phosphoshikimate 1-carboxyvinyltransferase), FT score -118.90, E-value 1.3e-07" FT rRNA 1341144..1342692 FT /class="II.A.6" FT /colour=4 FT /gene="16SrRNA" FT /note="16S ribosomal RNA gene. As assigned by Sela S., FT Clark-Curtiss J.E. Gene 98:123-127 (1991) (EMBL:M58020)" FT rRNA 1342976..1346100 FT /class="II.A.6" FT /colour=4 FT /gene="23SrRNA" FT /note="23S ribosomal RNA gene as assigned by Liesack W.C FT et al., (1991) FEBS Lett. 281:114-118" FT rRNA 1346186..1346301 FT /class="II.A.6" FT /colour=4 FT /gene="5SrRNA" FT /note="5S ribosomal RNA gene as assigned by Liesack W.C et FT al., (1991) FEBS Lett. 281:114-118" FT CDS complement(1346360..1346857) FT /class="II.A.5" FT /colour=2 FT /gene="ogt" FT /gene="ML1151" FT /note="Identical to the previously sequenced Mycobacterium FT leprae putative methylated-DNA--protein-cysteine FT methyltransferase (EC 2.1.1.63) SW:OGT_MYCLE (P52982) (165 FT aa); Fasta score E(): 0, 100.0% identity in 165 aa FT overlap. Also highly similar to many others e.g. FT Mycobacterium tuberculosis Rv1316c SW:OGT_MYCTU (Q10627) FT (165 aa); Fasta score E(): 0, 78.2% identity in 165 aa FT overlap and Bacillus subtilis SW:OGT_BACSU (P11742) (165 FT aa); Fasta score E(): 1e-20, 44.7% identity in 159 aa FT overlap. Contains Pfam match to entry PF01035 FT Methyltransf_1, 6-O-methylguanine DNA methyltransferase. FT Contains PS00374 Methylated-DNA--protein-cysteine FT methyltransferase active site." FT /product="probable methylated-DNA-protein-cysteine FT methyltransferase" FT /tb_orthologue="ogt" FT misc_feature complement(1346384..1346716) FT /colour=0 FT /note="Pfam match to entry PF01035 Methyltransf_1, FT 6-O-methylguanine DNA methyltransferase, score 243.10, FT E-value 3.8e-69" FT misc_feature complement(1346468..1346488) FT /colour=8 FT /note="PS00374 Methylated-DNA--protein-cysteine FT methyltransferase active site" FT CDS complement(1346877..1348313) FT /class="II.A.5" FT /colour=0 FT /gene="alkA" FT /gene="ML1152" FT /note="Possible pseudogene of M. tuberculosis orthologue FT alkA (Best blastx score 233)" FT /product="DNA-3-methyladenine glycosidase II (pseudogene)" FT /pseudo FT CDS 1348500..1349669 FT /class="I.B.7" FT /colour=0 FT /gene="ML1153" FT /note="Possible pseudogene similar to M. tuberculosis FT paralogue Rv0063 (Best blastx score 343)" FT /product="probable oxidoreductase (pseudogene)" FT /pseudo FT CDS complement(1349723..1350952) FT /class="V" FT /colour=0 FT /gene="ML1154" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1318c (Best blastx score 336)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT misc_feature 1351359..1352018 FT /colour=0 FT /note="Pfam match to entry PF01939 DUF91, Protein of FT unknown function, score 445.00, E-value 6.6e-130" FT CDS 1351359..1352021 FT /class="V" FT /colour=10 FT /gene="ML1155" FT /note="Previously sequenced Mycobacterium leprae FT hypothetical protein SW:YD21_MYCLE (P53524) (201 aa); FT Fasta score E(): 0, 99.5% identity in 193 aa overlap. Also FT highly similar to Mycobacterium tuberculosis hypothetical FT protein Rv1321 SW:YD21_MYCTU (Q10634) (226 aa); Fasta FT score E(): 0, 88.2% identity in 220 aa overlap. Contains FT Pfam match to entry PF01939 DUF91, Protein of unknown FT function." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv1321" FT CDS 1352045..1352328 FT /class="V" FT /colour=0 FT /gene="ML1156" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1322 (Best blastx score 222)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS complement(1352353..1352820) FT /class="V" FT /colour=10 FT /gene="ML1157" FT /ml_unique FT /note="Identical to the previously sequenced Mycobacterium FT leprae TR:Q49717 (EMBL:U00014) (155 aa); Fasta score E(): FT 0, 99.4% identity in 155 aa overlap. Also similar to FT Streptomyces coelicolor conserved hypothetical protein FT TR:CAB70628 (EMBL:AL137242) (146 aa); Fasta score E(): FT 1.5e-19, 42.1% identity in 140 aa overlap and Haemophilus FT influenzae lactoylglutathione lyase (EC 4.4.1.5) FT SW:LGUL_HAEIN (P44638) (135 aa); Fasta score E(): 0.69, FT 27.5% identity in 138 aa overlap. Contains Pfam match to FT entry PF01626 4HPPD_C, 4-hydroxyphenylpyruvate dioxygenase FT C terminal domain." FT /product="conserved hypothetical protein" FT misc_feature complement(1352362..1352820) FT /colour=0 FT /note="Pfam match to entry PF01626 4HPPD_C, FT 4-hydroxyphenylpyruvate dioxygenase C terminal domain, FT score 179.90, E-value 4e-50" FT misc_feature 1352912..1354090 FT /colour=0 FT /note="Pfam match to entry PF00108 thiolase, Thiolase, FT score 760.80, E-value 5.8e-225" FT CDS 1352912..1354093 FT /class="I.A.3" FT /colour=1 FT /gene="fadA4" FT /gene="ML1158" FT /note="Identical to the previously sequenced Mycobacterium FT leprae probable acetyl-CoA acetyltransferase (EC 2.3.1.9) FT SW:THIL_MYCLE (P46707) (393 aa); Fasta score E(): 0, FT 100.0% identity in 393 aa overlap. Also highly similar to FT many others including: acetyl-CoA acetyltransferases from FT Alcaligenes eutrophus SW:THIL_ALCEU (P14611) (393 aa); FT Fasta score E(): 0, 50.4% identity in 389 aa overlap and FT Mycobacterium tuberculosis (FadA4) Rv1323 SW:THIL_MYCTU FT (Q10629) (389 aa); Fasta score E(): 0, 86.9% identity in FT 388 aa overlap. Contains Pfam match to entry PF00108 FT thiolase, Thiolase. Contains PS00098 Thiolases acyl-enzyme FT intermediate signature. Contains PS00099 Thiolases active FT site. Contains PS00737 Thiolases signature 2." FT /note="ML2162" FT /product="possible acetyl-CoA C-acetyltransferase" FT /tb_orthologue="fadA4" FT misc_feature 1353161..1353217 FT /colour=8 FT /note="PS00098 Thiolases acyl-enzyme intermediate FT signature" FT misc_feature 1353926..1353976 FT /colour=8 FT /note="PS00737 Thiolases signature 2" FT misc_feature 1354031..1354072 FT /colour=8 FT /note="PS00099 Thiolases active site" FT CDS 1354280..1355185 FT /class="V" FT /colour=10 FT /gene="ML1159" FT /note="Previously sequenced Mycobacterium leprae TR:Q49716 FT (EMBL:U00014) (255 aa); Fasta score E(): 0, 100.0% FT identity in 217 aa overlap. Highly similar to FT Mycobacterium tuberculosis Rv1324 SW:YD24_MYCTU (Q10636) FT (304 aa); Fasta score E(): 0, 72.4% identity in 301 aa FT overlap. Also weakly similar in parts to several putative FT thioredoxins e.g. Alicyclobacillus acidocaldarius FT SW:THIO_ALIAC (P80579) (105 aa); Fasta score E(): 0.00045, FT 27.1% identity in 107 aa overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv1324" FT CDS complement(1355241..1357418) FT /class="II.A.8" FT /colour=0 FT /gene="ML1160" FT /note="Possible pseudogene of M. tuberculosis orthologue FT glgB (Best blastx score 959)" FT /product="1,4-[alpha]-glucan branching enzyme FT (pseudogene)" FT /pseudo FT CDS complement(1357444..1359540) FT /class="V" FT /colour=0 FT /gene="ML1161" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1327c (Best blastx score 650)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS 1360092..1362290 FT /class="II.A.8" FT /colour=0 FT /gene="ML1162" FT /note="Possible pseudogene of M. tuberculosis orthologue FT glgP (Best blastx score 579)" FT /product="possible glycogen phosphorylase (pseudogene)" FT /pseudo FT CDS complement(1363261..1364085) FT /class="IV.D" FT /colour=0 FT /gene="ML1163" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3239c (Best blastx score 223)" FT /product="possible antibiotic efflux protein (pseudogene)" FT /pseudo FT CDS complement(1364066..1364506) FT /class="V" FT /colour=0 FT /gene="ML1164" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1330c (Best blastx score 280)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS 1364612..1364938 FT /class="V" FT /colour=10 FT /gene="ML1165" FT /note="Previously sequenced Mycobacterium leprae FT hypothetical protein SW:YD31_MYCLE (P53423) (94 aa); Fasta FT score E(): 0, 98.9% identity in 94 aa overlap. Also highly FT similar to proteins of undefined function from FT Mycobacterium tuberculosis Rv1331 SW:YD31_MYCTU (Q10642) FT (101 aa); Fasta score E(): 0, 84.2% identity in 101 aa FT overlap and Streptomyces coelicolor TR:Q9S2G5 FT (EMBL:AL096852) (105 aa); Fasta score E(): 1.4e-20, 56.7% FT identity in 104 aa overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv1331" FT RBS 1364954..1364957 FT CDS 1364964..1365551 FT /class="V" FT /colour=10 FT /gene="ML1166" FT /note="Previously sequenced Mycobacterium leprae FT hypothetical protein SW:YD32_MYCLE (P53424) (222 aa); FT Fasta score E(): 0, 100.0% identity in 195 aa overlap. FT Also highly similar to proteins of undefined function from FT Mycobacterium tuberculosis Rv1332 SW:YD32_MYCTU (Q10643) FT (218 aa); Fasta score E(): 0, 74.4% identity in 195 aa FT overlap and Streptomyces coelicolor TR:Q9S2G6 FT (EMBL:AL096852) (202 aa); Fasta score E(): 1.5e-05, 34.6% FT identity in 188 aa overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv1332" FT CDS 1365551..1366639 FT /class="II.B.4" FT /colour=7 FT /gene="ML1167" FT /note="Previously sequenced Mycobacterium leprae FT hypothetical protein SW:YD33_MYCLE (P53425) (362 aa); FT Fasta score E(): 0, 100.0% identity in 362 aa overlap. FT Similar to Flavobacterium sp. TR:Q57326 (EMBL:D26094) (355 FT aa); Fasta score E(): 1.4e-11, 31.6% identity in 351 aa FT overlap endo-type 6-aminohexanoate oligomer hydrolase. FT Also similar to proteins of undefined function from FT Mycobacterium tuberculosis Rv1333 TR:YD33_MYCTU and FT Streptomyces coelicolor TR:CAB90977 (EMBL:AL355832) (348 FT aa); Fasta score E(): 0, 48.2% identity in 365 aa FT overlap." FT /product="possible hydrolase" FT /tb_orthologue="Rv1333" FT CDS 1366632..1366995 FT /class="V" FT /colour=0 FT /gene="ML1168" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1334 (Best blastx score 127)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS 1367134..1367390 FT /class="V" FT /colour=0 FT /gene="ML1169" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1335. Highly similar to Mycobacterium tuberculosis FT hypothetical protein Rv1335 9.5 KDa antigenic protein FT SW:CF10_MYCTU." FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT CDS 1367411..1368397 FT /class="I.D.3" FT /colour=0 FT /gene="cysM" FT /gene="ML1170" FT /note="Possible pseudogene of M. tuberculosis orthologue FT cysM (Best blastx score 302)" FT /product="cysteine synthase B (pseudogene)" FT /pseudo FT RBS 1368477..1368488 FT CDS 1368492..1369208 FT /class="II.C.4" FT /colour=3 FT /gene="ML1171" FT /note="Previously sequenced Mycobacterium leprae FT hypothetical protein SW:YD37_MYCLE (P53426) (251 aa); FT Fasta score E(): 0, 100.0% identity in 238 aa overlap. FT Also highly similar to other proteins of undefined FT function e.g. Mycobacterium tuberculosis hypothetical FT protein Rv1337 SW:YD37_MYCTU (Q10647) (240 aa); Fasta FT score E(): 0, 73.2% identity in 231 aa overlap. Contains FT possible membrane spanning hydrophobic domains. Contains FT Pfam match to entry PF01694 Rhomboid, Rhomboid family." FT /product="possible conserved integral membrane protein" FT /tb_orthologue="Rv1337" FT misc_feature 1368699..1369154 FT /colour=0 FT /note="Pfam match to entry PF01694 Rhomboid, Rhomboid FT family, score 158.10, E-value 1.6e-43" FT CDS 1369205..1370023 FT /class="II.C.3" FT /colour=3 FT /gene="murI" FT /gene="ML1172" FT /note="Identical to the previously sequenced Mycobacterium FT leprae probable glutamate racemase (EC 5.1.1.3) FT SW:MURI_MYCLE (P46705) (272 aa); Fasta score E(): 0, FT 100.0% identity in 272 aa overlap. Also highly similar to FT many others involved in peptidoglycan biosynthesis FT including glutamate racemases from Mycobacterium FT tuberculosis Rv1338 SW:MURI_MYCTU (Q10626) (271 aa); Fasta FT score E(): 0, 88.9% identity in 271 aa overlap and FT Bacillus subtilis SW:MURI_BACSU (P94556) (272 aa); Fasta FT score E(): 0, 42.2% identity in 268 aa overlap. Contains FT Pfam match to entry PF01177 Asp_Glu_race, Aspartate / FT glutamate racemase. Contains PS00924 Aspartate and FT glutamate racemases signature 2. Contains PS00923 FT Aspartate and glutamate racemases signature 1." FT /product="glutamate racemase" FT /tb_orthologue="murI" FT misc_feature 1369226..1369867 FT /colour=0 FT /note="Pfam match to entry PF01177 Asp_Glu_race, Aspartate FT / glutamate racemase, score 396.40, E-value 2.8e-115" FT misc_feature 1369418..1369444 FT /colour=8 FT /note="PS00923 Aspartate and glutamate racemases signature FT 1" FT misc_feature 1369745..1369777 FT /colour=8 FT /note="PS00924 Aspartate and glutamate racemases signature FT 2" FT CDS 1370096..1370878 FT /class="V" FT /colour=10 FT /gene="ML1173" FT /note="Previously sequenced Mycobacterium leprae FT hypothetical protein SW:YD39_MYCLE (P50474) (284 aa); FT Fasta score E(): 0, 100.0% identity in 260 aa overlap. FT Also highly similar to several other proteins of unknown FT function e.g. Mycobacterium tuberculosis Rv1339 FT SW:YD39_MYCTU and Streptomyces coelicolor TR:Q9S2H3 FT (EMBL:AL096852) (250 aa); Fasta score E(): 0, 43.0% FT identity in 256 aa overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv1339" FT CDS 1370968..1371747 FT /class="II.B.1" FT /colour=7 FT /gene="rphA" FT /gene="ML1174" FT /note="Identical to the previously sequenced Mycobacterium FT leprae probable ribonuclease PH (EC 2.7.7.56) FT SW:RNPH_MYCLE (P37939) (259 aa); Fasta score E(): 0, 99.6% FT identity in 259 aa overlap. Also highly similar to many FT other ribonucleases including: Mycobacterium tuberculosis FT SW:RNPH_MYCTU (Q10628) (259 aa); Fasta score E(): 0, 88.8% FT identity in 259 aa overlap and Escherichia coli FT SW:RNPH_ECOLI (P03842) (238 aa); Fasta score E(): 0, 55.7% FT identity in 237 aa overlap. Contains Pfam match to entry FT PF01138 RNase_PH, 3' exoribonuclease family. Contains FT PS01277 Ribonuclease PH signature." FT /product="ribonuclease PH" FT /tb_orthologue="rphA" FT misc_feature 1370977..1371648 FT /colour=0 FT /note="Pfam match to entry PF01138 RNase_PH, 3' FT exoribonuclease family, score 286.30, E-value 3.9e-82" FT misc_feature 1371319..1371357 FT /colour=8 FT /note="PS01277 Ribonuclease PH signature" FT CDS 1371782..1372444 FT /class="V" FT /colour=10 FT /gene="ML1175" FT /note="Previously sequenced Mycobacterium leprae FT hypothetical protein TR:Q9S383 (EMBL:AL049191) (220 aa); FT Fasta score E(): 0, 99.5% identity in 220 aa overlap. Also FT highly similar to many other proteins of undefined FT function including: Mycobacterium tuberculosis Rv1341 FT SW:YD41_MYCTU (Q10649) (204 aa); Fasta score E(): 0, 82.4% FT identity in 205 aa overlap and Escherichia coli FT SW:YGGV_ECOLI (P52061) (197 aa); Fasta score E(): 1e-23, FT 44.8% identity in 203 aa overlap. Contains Pfam match to FT entry PF01725 Ham1p_like, Ham1 family." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv1341" FT misc_feature 1371836..1372420 FT /colour=0 FT /note="Pfam match to entry PF01725 Ham1p_like, Ham1 FT family, score 337.60, E-value 1.4e-97" FT CDS complement(1372485..1372844) FT /class="II.C.4" FT /colour=3 FT /gene="ML1176" FT /note="Identical to the previously sequenced Mycobacterium FT leprae hypothetical protein SW:YD42_MYCLE (P54133) (119 FT aa); Fasta score E(): 0, 100.0% identity in 119 aa FT overlap. Also highly similar to hypothetical proteins from FT Mycobacterium tuberculosis Rv1342c TR:YD42_MYCTU and FT Streptomyces coelicolor TR:Q9X948 (EMBL:AJ237976) (109 FT aa); Fasta score E(): 7.7e-11, 36.5% identity in 96 aa FT overlap. Contains possible membrane spanning hydrophobic FT domains." FT /product="possible conserved membrane protein" FT /tb_orthologue="Rv1342c" FT CDS complement(1372841..1373221) FT /class="II.C.1" FT /colour=3 FT /gene="ML1177" FT /note="Identical to the previously sequenced Mycobacterium FT leprae hypothetical protein SW:YD43_MYCLE (P54134) (126 FT aa); Fasta score E(): 0, 99.2% identity in 126 aa overlap. FT Also highly similar to Mycobacterium tuberculosis Rv1343c FT SW:YD43_MYCTU (Q11013) (126 aa); Fasta score E(): 0, 78.4% FT identity in 125 aa overlap. Contains a possible N-terminal FT signal sequence and an appropriately positioned FT Prokaryotic membrane lipoprotein lipid attachment site. FT Contains PS00013 Prokaryotic membrane lipoprotein lipid FT attachment site." FT /product="possible lipoprotein" FT misc_feature complement(1373162..1373194) FT /colour=8 FT /note="PS00013 Prokaryotic membrane lipoprotein lipid FT attachment site" FT tRNA 1374076..1374158 FT /colour=4 FT /note="tRNA Leu anticodon TAG, Cove score 55.29" FT CDS 1374947..1375675 FT /class="I.H.1" FT /colour=0 FT /gene="ML1178" FT /note="Possible pseudogene of M. tuberculosis orthologue FT fabG2 (Best blastx score 176)" FT /product="possible 3-oxoacyl-[ACP] reductase (pseudogene)" FT /pseudo FT CDS 1377207..1378301 FT /class="I.B.7" FT /colour=0 FT /gene="ML1179" FT /product="possible acyl-CoA dehydrogenase (pseudogene)" FT /pseudo FT repeat_unit complement(1380273..1381197) FT /note="97 % identical to bases 1221172..1222101" FT CDS complement(1380300..1380587) FT /class="V" FT /colour=10 FT /gene="ML1180" FT /note="Identical to the previously sequenced Mycobacterium FT leprae hypothetical protein TR:Q49946 (EMBL:U15180) (95 FT aa); Fasta score E(): 0, 100.0% identity in 95 aa overlap. FT Also similar to many Mycobacterium tuberculosis proteins FT with undefined function e.g. RV1793 TR:O53942 FT (EMBL:AL022021) (94 aa); Fasta score E(): 4.3e-23, 65.2% FT identity in 92 aa overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv2346c" FT RBS complement(1380598..1380603) FT CDS complement(1380639..1380941) FT /class="V" FT /colour=10 FT /gene="ML1181" FT /note="Identical to the previously sequenced Mycobacterium FT leprae hypothetical protein TR:Q49945 (EMBL:U15180) (100 FT aa); Fasta score E(): 0, 100.0% identity in 100 aa FT overlap. Also similar to many Mycobacterium tuberculosis FT proteins with undefined function e.g. RV1197 TR:O05299 FT (EMBL:Z93777) (98 aa); Fasta score E(): 1.7e-20, 58.3% FT identity in 96 aa overlap." FT /product="conserved hypothetical protein" FT CDS complement(1381004..1382269) FT /class="V" FT /colour=6 FT /gene="ML1182" FT /note="Identical to the previously sequenced Mycobacterium FT leprae putative PPE protein TR:Q9Z5K0 (EMBL:AL049191) (421 FT aa); Fasta score E(): 0, 100.0% identity in 421 aa FT overlap. Also highly similar to many members of the FT Mycobacterium tuberculosis PPE-family of proteins e.g. FT Rv2892c SW:YS92_MYCTU (Q10813) (408 aa); Fasta score E(): FT 1.2e-25, 34.0% identity in 429 aa overlap. Contains Pfam FT match to entry PF00823 PPE, PPE family." FT /product="PPE-family protein" FT repeat_unit complement(1381745..1382838) FT /note="99 % identical to bases 1220160..1221253" FT misc_feature complement(1381781..1382254) FT /colour=0 FT /note="Pfam match to entry PF00823 PPE, PPE family, score FT 312.90, E-value 3.8e-90" FT CDS complement(1382326..1382625) FT /class="V" FT /colour=10 FT /gene="ML1183" FT /note="Identical to the previously sequenced Mycobacterium FT leprae hypothetical protein TR:Q49943 (EMBL:AL049191) (99 FT aa); Fasta score E(): 0, 100.0% identity in 99 aa overlap. FT Also highly similar to many Mycobacterium tuberculosis FT hypothetical proteins e.g. TR:O05297 (EMBL:Z93777) (99 FT aa); Fasta score E(): 1.3e-12, 48.9% identity in 92 aa FT overlap and the N-terminus of several PGRS-family FT proteins, also from M. tuberculosis e.g. RV0742 TR:O53805 FT (EMBL:AL021958) (175 aa); Fasta score E(): 8.8e-09, 39.6% FT identity in 96 aa overlap. Contains Pfam match to entry FT PF00934 PE, PE family." FT /product="conserved hypothetical protein" FT misc_feature complement(1382341..1382622) FT /colour=0 FT /note="Pfam match to entry PF00934 PE, PE family, score FT 155.60, E-value 8.6e-43" FT CDS complement(1383635..1384713) FT /class="I.B.7" FT /colour=0 FT /gene="ML1184" FT /note="Possible pseudogene of M. tuberculosis orthologue FT adhD (Best blastx score 217)" FT /product="zinc-containing alcohol dehydrogenase FT (pseudogene)" FT /pseudo FT CDS complement(1384839..1385327) FT /class="IV.F" FT /colour=0 FT /gene="ML1185" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv1777 (Best blastx score 111)" FT /product="probable cytochrome p450 (pseudogene)" FT /pseudo FT CDS complement(1386776..1387078) FT /class="VI" FT /colour=8 FT /gene="ML1186" FT /ml_unique FT /note="unknown function." FT /product="hypothetical protein" FT CDS complement(1387515..1388406) FT /class="I.A.3" FT /colour=0 FT /gene="ML1187" FT /note="Possible pseudogene of M. tuberculosis orthologue FT fadD8 (Best blastx score 98)" FT /product="acyl-CoA synthase (pseudogene)" FT /pseudo FT CDS complement(1390595..1390855) FT /class="VI" FT /colour=8 FT /gene="ML1188" FT /ml_unique FT /note="unknown function." FT /product="hypothetical protein" FT CDS complement(1392493..1392876) FT /class="VI" FT /colour=8 FT /gene="ML1189" FT /ml_unique FT /note="unknown function, identical to the previously FT sequenced Mycobacterium leprae hypothetical protein FT TR:Q9X7D8." FT /product="hypothetical protein" FT CDS 1395291..1396010 FT /class="V" FT /colour=10 FT /gene="ML1190" FT /note="Identical to the previously sequenced Mycobacterium FT leprae hypothetical protein TR:Q9X7E1 (EMBL:AL049478) (239 FT aa); Fasta score E(): 0, 100.0% identity in 239 aa FT overlap. Also highly similar to Mycobacterium tuberculosis FT Rv2525c TR:P95028 (EMBL:Z83863) (240 aa); Fasta score E(): FT 0, 82.2% identity in 241 aa overlap." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv2525c" FT CDS 1396622..1405852 FT /class="I.H.1" FT /colour=1 FT /gene="fas" FT /gene="ML1191" FT /note="Identical to the previously sequenced Mycobacterium FT leprae putative type I fatty acid synthase TR:Q9X7E2 FT (EMBL:AL049478) (3076 aa); Fasta score E(): 0, 100.0% FT identity in 3076 aa overlap. Also highly similar to many FT other fatty acid synthases including: Mycobacterium FT tuberculosis Rv2524c TR:P95029 (EMBL:Z83863) (3069 aa); FT Fasta score E(): 0, 85.8% identity in 3081 aa overlap and FT Corynebacterium ammoniagenes fatty-acid synthase (EC FT 2.3.1.85) TR:Q59497 (EMBL:X87822) (3063 aa); Fasta score FT E(): 0, 44.3% identity in 3106 aa overlap. Contains Pfam FT match to entry PF00109 ketoacyl-synt, Beta-ketoacyl FT synthase. Contains Pfam match to entry PF00698 FT Acyl_transf, Acyl transferase domain. Contains Pfam match FT to entry PF01575 MaoC_dehydratas, MaoC like domain. FT Contains PS00013 Prokaryotic membrane lipoprotein lipid FT attachment site. Contains PS00606 Beta-ketoacyl synthases FT active site." FT /product="fatty acid synthase" FT /tb_orthologue="fas" FT misc_feature 1400222..1400590 FT /colour=0 FT /note="Pfam match to entry PF01575 MaoC_dehydratas, MaoC FT like domain, score 136.40, E-value 5e-37" FT misc_feature 1400621..1401502 FT /colour=0 FT /note="Pfam match to entry PF00698 Acyl_transf, Acyl FT transferase domain, score 19.00, E-value 4.2e-11" FT misc_feature 1404284..1405615 FT /colour=0 FT /note="Pfam match to entry PF00109 ketoacyl-synt, FT Beta-ketoacyl synthase, score -125.00, E-value 3.1e-06" FT misc_feature 1404773..1404823 FT /colour=8 FT /note="PS00606 Beta-ketoacyl synthases active site" FT CDS 1405881..1406273 FT /class="I.H.1" FT /colour=1 FT /gene="acpS" FT /gene="ML1192" FT /note="Identical to the previously sequenced Mycobacterium FT leprae holo-[acyl-carrier protein] synthase (ACP)(EC FT 2.7.8.7) SW:ACPS_MYCLE (Q9X7E3) (130 aa); Fasta score E(): FT 0, 99.2% identity in 130 aa overlap. Also highly similar FT to ACPs from Mycobacterium tuberculosis SW:ACPS_MYCTU FT (O53228) (130 aa); Fasta score E(): 0, 86.7% identity in FT 128 aa overlap and Escherichia coli SW:ACPS_ECOLI (P24224) FT (125 aa); Fasta score E(): 0.00025, 35.1% identity in 131 FT aa overlap. Contains Pfam match to entry PF01648 ACPS, FT 4'-phosphopantetheinyl transferase superfamily." FT /product="holo-[acyl-carrier protein] synthase" FT /tb_orthologue="acpS" FT misc_feature 1405893..1406252 FT /colour=0 FT /note="Pfam match to entry PF01648 ACPS, FT 4'-phosphopantetheinyl transferase superfamily, score FT 126.70, E-value 4.3e-34" FT CDS 1406363..1407691 FT /class="V" FT /colour=10 FT /gene="ML1193" FT /note="Identical to the previously sequenced Mycobacterium FT leprae hypothetical protein TR:Q9X7E4 (EMBL:AL049478) (442 FT aa); Fasta score E(): 0, 100.0% identity in 442 aa overlap FT and highly similar to Mycobacterium tuberculosis FT hypothetical protein Rv2522c TR:O53227 (EMBL:AL021185) FT (470 aa); Fasta score E(): 0, 86.4% identity in 440 aa FT overlap. Contains Pfam match to entry PF01546 FT Peptidase_M20, Peptidase family M20/M25/M40." FT /product="conserved hypothetical protein" FT /tb_orthologue="Rv2522c" FT misc_feature 1406417..1407523 FT /colour=0 FT /note="Pfam match to entry PF01546 Peptidase_M20, FT Peptidase family M20/M25/M40, score 193.20, E-value FT 1.8e-54" FT repeat_unit 1407616..1408469 FT CDS complement(1408807..1410072) FT /class="V" FT /colour=0 FT /gene="ML1194" FT /note="Possible pseudogene of M. tuberculosis orthologue FT papA3 (Best blastx score 212)" FT /product="conserved hypothetical protein (pseudogene)" FT /pseudo FT RBS 1410776..1410780 FT CDS 1410785..1413964 FT /class="II.A.3" FT /colour=2 FT /gene="ileS" FT /gene="ML1195" FT /note="Identical to the previously sequenced Mycobacterium FT leprae isoleucyl-tRNA synthetase (EC 6.1.1.5) SW:SYI_MYCLE FT (Q9X7E5) (1059 aa); Fasta score E(): 0, 100.0% identity in FT 1059 aa overlap. Also highly similar to many Prokaryotic FT and Eukaryotic tRNA synthases e.g. Mycobacterium FT tuberculosis RV1536 SW:SYI_MYCTU (Q10765) (1041 aa); Fasta FT score E(): 0, 84.4% identity in 1059 aa overlap and FT Schizosaccharomyces pombe SW:SYIC_SCHPO (O13651) (1064 FT aa); Fasta score E(): 0, 39.5% identity in 1041 aa FT overlap. Contains Pfam match to entry PF00133 tRNA-synt_1, FT tRNA synthetases class I (I, L, M and V). Contains PS00178 FT Aminoacyl-transfer RNA synthetases class-I signature." FT /note="Similar to ML1472 (valyl-tRNA synthase)" FT /product="isoleucyl-tRNA synthase" FT /tb_orthologue="ileS" FT misc_feature 1410866..1412986 FT /colour=0 FT /note="Pfam match to entry PF00133 tRNA-synt_1, tRNA FT synthetases class I (I, L, M and V), score 690.10, E-value FT 1.1e-203" FT misc_feature 1410959..1410994 FT /colour=8 FT /note="PS00178 Aminoacyl-transfer RNA synthetases class-I FT signature" FT CDS complement(1414046..1414552) FT /class="V" FT /colour=0 FT /gene="ML1196" FT /note="Possible pseudogene of M. tuberculosis orthologue FT Rv3832c (Best blastx score 159)" FT /product="hypothetical protein (pseudogene)" FT /pseudo FT CDS 1414678..1416054 FT /class="II.A.5" FT /colour=0 FT /gene="dinX" FT /gene="ML1197" FT /note="Possible pseudogene of M. tuberculosis orthologue FT dinX (Best blastx score 489)" FT /product="probable DNA-damage-inducible protein FT (pseudogene)" FT /pseudo FT CDS complement(1416022..1416954) FT /class="I.A.2" FT /colour=7 FT /gene="ansA" FT /gene="ML1198" FT /note="Identical to the previously sequenced Mycobacterium FT leprae probable L-asparaginase (EC 3.5.1.1) SW:ASPG_MYCLE FT (Q9X7E6) (310 aa); Fasta score E(): 0, 100.0% identity in FT 31