A detailed protocol for thermosensitive mutants generation by PCR (used for yeast Mak11).

Preparing total RNA from S. cerevisiae cells and performing Real-Time PCR reactions is relatively straightforward (protocol page).

Performing a polysome profile experiment.

Two-hybrid screens in yeast.

Primer extension for mapping 5′ RNA ends.

More protocols will become available as soon as we can put them in the right format.

2013-10-03 10:20:00