Tel + (33) 1 40 61 34 18
Fax+ (33) 1 45 68 80 07
Tel + (33) 1 40 61 39 17
Fax+ (33) 1 40 61 39 70
Tel + (33) 1 40 61 39 17
Fax+ (33) 1 40 61 39 70

Tel + (33) 1 40 61 39 17
Fax+ (33) 1 40 61 39 70

 (Underlined names = external collaborators)

•  Chauhan, A.; Bernardin, A.;  Mussard, W.; Kriegel, I.; Esteve, M.; Ghigo, J.M.; Beloin*, C.; and V. Semetey*. (2014) In vivo inhibition of bacterial biofilm development on totally implantable venous catheters by biomimetic glycocalyx-like polymers. The Journal of Infectious Diseases in press

•  Zaïri, A. ; Ferrières, L.; Latour-Lambert, P. ; Beloin, C. ; Tangy, F. ; Ghigo, J.M. and K. Hani(2014) In vitro activity of Dermaseptins K4S4 and K4K20S4 against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa planktonic growth and biofilm formation. Antimicrobial Agents Chemotherapy in press

Jacquot A., Sakamoto C., Razafitianamaharavo. A., Merlin J., Fahs A., Ghigo J.M., Beloin C.#, Duval J.F.L.# and G. Francius. (2014) Dynamic modulation of fimbrial extension and FimH-Mannose binding force on live bacteria under pH changes: a molecular AFM analysis. J of Biomedicine & NanotechnologyIn press.   equivalent contribution

Zrelli, K.; Galy, O.; Latour-Lambert, P.; Kirwan, L.; Ghigo, J.M.; Beloin, C. and N. Henry (2014) Bacterial biofilm mechanical properties persist upon antibiotic treatment and survive cell death. New Journal of Physics  in press

• Balloy, V.; Deveaux, A.; Lebeaux, D.; Tabary, O.; Le Rouzic, P. ; Ghigo, J.M. ; Boëlle, P.Y., Clement, A. Chignard, M.; Corvol, H. ; Burnet, M. and  L. Guillot. (2014). Azithromycin Analog CSY0073 Attenuates Lung Inflammation induced by Lipopolysaccharide Challenge British Journal of Pharmacology in press 

• Rendueles, O.; Beloin, C.; Latour-Lambert, P. and Ghigo J.M.. (2014). A new biofilm-associated colicin targeting E. coli biofilm bacteria The ISME Journal. in press

• Létoffé, S.; Audrain, B.; Bernier, S. ; Delepierre, M and Ghigo J.M.(2014)Aerial exposure to the bacterial volatile compound trimethylamine modifies antibiotic resistance of physically separated bacteria by raising medium pH. mBio Jan 7;5(1). pii: e00944-13.

• Lebeaux, D.; Fernández-Hidalgo, N. ; Chauhan, A. ; Lee, S. ; Ghigo, J.M. Almirante, B.  and C.Beloin (2014). Management of totally implantable venous access port-related infections: challenges and perspectives in revision for The Lancet Infectious Diseases Feb;14(2):146-59.

Audrain, B.; Ferrières, L.; Zairi, A.; Soubigou G., Dobson, C. ;J.-Y. Coppée; Beloin, C. and J.M. Ghigo. (2013) CpxAR two-component system contributes to resistance against cationic antimicrobial peptides in E. coli. Appl. Environl Microbiol. Dec;79(24):7770-9. doi: 10.1128.

Siriwardena, A.; Pulukuri,K. K; Kandiyal,P.S.; Roy, S.; Bande, O.; Martin, F.A.; Ghigo J.M.; Beloin, C.; Ghosh, S. Ampapathi, S.S. and KK. Chakraborty (2013) Carbohydrate-modified foldamers as conformationally defined and biologically distinct glycopeptide mimics. Angewandte Chemie Int Ed Sep 23;52(39):10221-6

Chauhan* A.; Sakamoto*, S.; Ghigo J.M. and C. Beloin (2013) Did I pick the right colony? Phase variation pitfalls in the study of regulation of phase variable antigen 43 adhesin PLoS One Sep 5;8(9):e73568.* equal contribution

Travier, L., Rendueles, O.; Ferrières, L.; Herry, J.M. and J.M. Ghigo. (2013) Escherichia coli resistance to non-biocidal anti-biofilm polysaccharide is rare and mediated by multiple mutations leading to surface charge modifications. Antimicrobial Agents ChemotherapyAug;57(8):3960-8

Da Re S.; Valle, J.,  Charbonnel, N.; Beloin, C.; Latour-Lambert, P.; Faure, P.; Turlin, E.; Le Bouguenec C; Renauld –Mongénie, G; Forestier, C. and J.M. Ghigo. (2013) Identification of commensal Escherichia coli genes involved in biofilm resistance to pathogen colonization. PloS ONE. May 7;8(5):e61628.

• F.A.Martin* ; A.Barras*; O. Bande; J.M. Ghigo ; Boukherroub, R. ; C. Beloin ; A. Siriwardena, S. Szunerits. (2013) Glyconanodialond as type 1 fimbriae inhibitors to limit bacterial adhesion. Nanoscale. Feb 28;5(6):2307-16.* equal contribution

• Travier, L., Guadagnini, S.; Gouin, E.; Dufour, A.; Chenal-Francisque V.; Leclercq, A.; Cossart, Ghigo, J.M.; P., Disson, O. and Marc Lecuit.(2013) ActA promotes Listeria monocytogenes aggregation, intestinal colonization and transmission, independently of its role in virulence. PLoS Pathogens.Jan;9(1):e1003131.

• Bernier, S.; Lebeaux, D.; DeFrancesco, A Valomon, A.; Soubigou, G.; , Coppee, J.Y.; Ghigo, J.M. and C. Beloin (2013) Starvation associated with the SOS response mediate high and biofilm-specific tolerance to the fluoroquinolone ofloxacin PLoS Genetics Jan;9(1):e1003144.

• Chauhan A.; Lebeaux, D.; Ghigo J.M. and C. Beloin. (2012) Full and broad-spectrum eradication of catheter-associated in vivo biofilms using Gentamicin-EDTA antibiotic lock therapy. Submitted to Antimicrobial Agents Chemotherapy 56(12):6310-8.

Allsopp, L.P.; Beloin, C.; Totsika, M.;  Ghigo, J.M. and M.A. Schembri.(2012) Functional heterogeneity of the UpaH autotransporter protein from uropathogenic Escherichia coli. J. Bacteriol 194(21):5769-82.

Galy, O. ; Latour-Lambert, P. ; Zrelli, K. ; Ghigo, J.M.; Beloin, C. and N.Henry.(2012) Mapping of bacterial biofilm local mechanics using magnetic microparticles The Biophysical Journal19;103(6):1400-8.

Rendueles, O.; Ferrieres, L.; Fretaud, M.; Begaud, E.; Hebomel, P.; Levraud, J.P. and J.M. Ghigo. (2012) A new zebrafish model of oro-intestinal pathogen colonization reveals a key role for adhesion in protection by probiotic bacteria. PLoS Pathogens.Jul;8(7):e1002815..

• Rendueles, O; Kaplan, JB; Ghigo, J.M. (2012) Antibiofilm polysaccharides Minireview. Environmental Microbiology Feb;15(2):334-46..

Prigent-Combaret, C. ;Sanguin, H. ; Champier, L. ; Bertrand, C. ; Monnez, C. ;Colinon, C. ; Blaha, D. ; Ghigo, J.M. and B. Cournoyer (2012) The bacterial thiopurine methyltransferase tellurite resistance process is highly dependent upon aggregation properties and oxidative stress response Environmental Microbiology14(10):2645-60

• Lebeaux, D. et J.M. Ghigo. (2012) Infections associées aux biofilms : quelles perspectives thérapeutiques issues de la recherche fondamentale? (In french) Médecine/Sciences Aug-Sep;28(8-9):727-39

• Chauhan A.; Lebeaux, D.; Decante, B.;  Kriegel, I.; Escande, M.C.; Ghigo J.M. and C. Beloin. (2012) A rat model of long-term indwelling device reveals the importance of immune system in controlling biofilm establishment and related infections.PloS ONE. 2012;7(5):e37281

• Totsika, M.; Wells, T.J.; Beloin, C.;Valle, J ; Allsopp, L.P.; King, N.   Ghigo, J.M. and M.A. Schembri. (2012) Molecular characterisation of the EhaG and UpaG trimeric autotransporter proteins from pathogenic Escherichia coli. Appl Environ Microbiol.78(7):2179-89.

• Rendueles, O. and J.M. Ghigo Multi-species biofilms: How to avoid unfriendly neighbors. (2012). FEMS Microbiology Reviews Epub ahaed of print

(• Allsopp, L.P. ; Beloin, C., Ulett, G.C. ;Valle, J ; Totsika, M. ; Sherlock, O., Ghigo, J.M. and M.A. Schembri. (2012) Molecular Characterisation of UpaB and UpaC - two new Autotransporter Proteins of Uropathogenic Escherichia coli CFT073. Infection and Immunity 80(1):321-32.

• Bernier, S. ; Létoffé, S. ; Delepierre, M and Ghigo J.M.. (2011) Biogenic ammonia modifies antibiotic resistance at a distance in physically separated bacteria. Molecular Microbiology 81:705-716.

Francius, G. ; Poliakov, P. ; Merlin, J. ; Abe, Y. ; Ghigo, J.M. ; Merlin, C. ; Beloin, C. and J. Duval.(2011) Bacterial surface appendages strongly impact on nanomechanical and electrokinetic properties of Escherichia coli cells subjected to osmotic stress. PLoS ONE 6(5):e20066.

• Rendueles, O. ; Travier, L. ; Latour-Lambert, P. ; Fontaine, T. ; Magnus, J. ; Denamur, E.  and Ghigo J.M. (2011) Screening Escherichia coli species biodiversity reveals new biofilm-associated anti-adhesion polysaccharides. mBio 00043-11

• Korea, C. ; J. M. Ghigo and C. Beloin. (2011) The sweet connection: solving the riddle of multiple sugar-binding fimbrial adhesins in Escherichia coli Biessays . vol. 33 (4) pp. 300-11

Ferrieres, L., Hemery, G., Nham, T., Guerout, A. M., Mazel, D., Beloin, C. and Ghigo, J. M. (2010) Silent mischief: bacteriophage Mu insertions contaminate products of Escherichia coli random mutagenesis performed using suicidal transposon delivery plasmids mobilized by broad-host-range RP4 conjugative machinery  J Bacteriol.192:6418-27..

Korea C.; Badouraly R.; Prevost, M.C.; Ghigo J.M. and C.Beloin. (2010). E. coli K-12 possesses multiple cryptic but functional chaperone-usher fimbriae with distinct surface specificities. Environ Microbiol.12:1957-77.

• Cristina D. Ciornei, Alexey Novikov, Christophe Beloin, Catherine Fitting, Martine Caroff , Jean-Marc Ghigo, Jean-Marc Cavaillon And Minou Adib-Conquy.(2010).Biofilm-forming pseudomonas aeruginosa bacteria undergo lps structural modifications that induce enhanced inflammatory cytokine response in human monocytes. Innate Immun.16:288-301.

Le Quéré, B and J.M. Ghigo (2009). BcsQ is an essential component of the Escherichia coli cellulose biosynthesis apparatus that localizes at the bacterial cell pole.’ Molecular Microbiology 72(3), 724–740

Touchon, M. ; Hoede, C. ; Tenaillon, o. et al (2008) Organised genome dynamics in the Escherichia coli species: the path to adaptation. PLoS Genetics 5(1) e1000344

Beloin, C.; Houry, A;, Froment , M. ; Ghigo J.M. and N. Henry. A short time scale colloidal system reveals early bacterial adhesion dynamics and  adhesin-dependent behaviours. (2008) PLoS Biology July | Volume 6 | Issue 7 | e167.


 Collet, A. ; Cosette, P.; Beloin, C.; Ghigo, J.M.; Rihouey, C.; Lerouge, P.; Junter, G.A. And T. Jouenne.  Impact of rpoS deletion on the proteome of Escherichia coli grown as biofilm. (2008) Journal of Proteomic Research 7(11):4659-4669.

Beloin, C., Roux, A. and Ghigo, J. M. (2008) Escherichia coli biofilms  Curr Top Microbiol Immunol.322:249-89.

Valle, J., Mabbett, A. N., Ulett, G. C., Toledo-Arana, A., Wecker, K., Totsika, M., Schembri, M. A., Ghigo, J. M. and Beloin, C. ( 2008) UpaG, a new member of the trimeric autotransporter family of adhesins in uropathogenic Escherichia coli  J Bacteriol.190:4147-61.

Valle, J. : Da Re, S., Schmid. S. ; Skurnik, D. ; D’Ari, R. and Ghigo J.M. The amino-acid valine is secreted in continuous-flow bacterial biofilms. (2008) J. Bacteriol. 190(1):264-74.

Balestrino, D.; Ghigo J.M. ; Charbonnel, N. ; Haagensen, J.A.J. and C. Forestier. Individual and competitive screening of Klebsiella  pneumoniae factors involved in the establishment and maturation of in vitro biofilm. (2008) Environmental  Microbiology 10(3), 685–70141

•  Ulett, G.C. ; Valle, J. ; Beloin, C. ; Sherlock, O. ; Ghigo, J.M. and M. A. Schembri. (2007). Functional analysis of Antigen 43 in uropathogenic Escherichia coli reveals a role in long term persistence in the urinary tract. Infection and Immunity 75 3233-3244

 Da Re, S., Le Quere, Ghigo, J.M. and C. Beloin .(2007). Tight modulation of Escherichia coli bacterial biofilm formation through controlled expression of adhesion factors. Appl. Environl Microbiol. 73:3391-403.

 Valle, J. : Da Re, S., Henry N. ; Fontaine, T.; Balestrino, D.; Latour-Lambert, P.; and Ghigo J.M. (2006) Broad-spectrum biofilm inhibition by a secreted bacterial polysaccharide. Proc Natl Acad Sci U S 103:12558-12563.

Da Re, S. and Ghigo, J. M. (2006) A CsgD-Independent Pathway for Cellulose Production and Biofilm Formation in Escherichia coli J Bacteriol.188:3073-87.

E. G. Dudley, C. Abe, J. M. Ghigo, P. Latour-Lambert, J. C. Hormazabal and J. P. Nataro. (2006) An IncI1 Plasmid Contributes to the Adherence of the Atypical Enteroaggregative Escherichia coli Strain C1096 to Cultured Cells and Abiotic Surfaces Infect Immun.74:2102-14.

C. Beloin, K. Michaelis, K. Lindner, P. Landini, J. Hacker, J. M. Ghigo and U. Dobrindt. (2006) The Transcriptional Antiterminator RfaH Represses Biofilm Formation in Escherichia coli J Bacteriol.188:1316-31.

C. Latasa, A. Roux, A. Toledo-Arana, J. M. Ghigo, C. Gamazo, J. R. Penades and I. Lasa. (2005) BapA, a large secreted protein required for biofilm formation and host colonization of Salmonella enterica serovar Enteritidis Mol Microbiol.58:1322-39.

 • C. Beloin, S. Da Re and J. M. Ghigo. (2005) Chapter Colonization of abiotic surfaces Escherichia coli and Salmonella. Cellular and Molecular Biology. [on line]. ASM Press, Washington, D.C.

Jubelin, G., Vianney, A., Beloin., Ghigo, J.M., Lazzaroni, J.C., Lejeune, P. and C..  Dorel (2005). CpxR/OmpR Interplay regulates curli gene expression in response to osmolarity in Escherichia coli. J. Bacteriol. 187 2038-2049

Iraqui,I. ;Aubert, S. ;Dromer, F. ; Ghigo, J.M. D'enfert, C. And G. Janbon (2005) Epa6p Is A Major Adhesin Responsible For Biofilm Formation In Candida Glabrata Mol Microbiol. 55:1259-71

Roux, A, Beloin, C. and Ghigo, J.M.(2005) A combined inactivation/expression strategy to study gene function in physiological conditions: application to the identification of new adhesins in E. coli. J. Bacteriol.187:1001-1013.

C. Beloin and J. M. Ghigo. (2005) Finding gene-expression patterns in bacterial biofilms Trends Microbiol.13:16-9.

Beloin, C; J. Valle, P. Latour-Lambert, P. Faure, M. Kzreminski, D. Balestrino, J. A. Haagensen, S. Molin, G. Prensier, B. Arbeille and J. M. Ghigo. (2004) Global impact of mature biofilm lifestyle on Escherichia coli K-12 gene expression Mol Microbiol.51:659-74.

Garcia-Sanchez, S.; S. Aubert, I. Iraqui, G. Janbon, J. M. Ghigo and C. d'Enfert. (2004) Candida albicans biofilms: a developmental state associated with specific and stable gene expression patterns Eukaryot Cell.3:536-45

  J. M. Ghigo. (2003.) Are there biofilm-specific physiological pathways beyond a reasonable doubt? Res Microbiol.154:1-8.

  J. Valle, A. Toledo-Arana, C. Berasain, J. M. Ghigo, B. Amorena, J. R. Penades and I. Lasa. (2003.) SarA and not sigmaB is essential for biofilm development by Staphylococcus aureus Mol Microbiol.48:1075-87.

 A. Toussaint, J. M. Ghigo and G. P. Salmond. (2003.) A new evaluation of our life-support system EMBO Rep.4:820-4.

C. Solano, B. Garcia, J. Valle, C. Berasain, J. M. Ghigo, C. Gamazo and I. Lasa. (2002.) Genetic analysis of Salmonella enteritidis biofilm formation: critical role of cellulose Mol Microbiol.43:793-808.

• J. M. Ghigo. (2001.) Natural conjugative plasmids induce bacterial biofilm development Nature.412:442-5.


 • Ferrieres, L., Hemery, G., Nham, T., Guerout, A. M., Mazel, D., Beloin, C. and Ghigo, J. M. (2010) Silent mischief: bacteriophage Mu insertions contaminate products of Escherichia coli random mutagenesis performed using suicidal transposon delivery plasmids mobilized by broad-host-range RP4 conjugative machinery J Bacteriol.192:6418-27.

Beloin, C., Houry, A., Froment, M., Ghigo, J. M. and Henry, N. (2008) A short-time scale colloidal system reveals early bacterial adhesion dynamics PLoS Biol.6:e167.

Da Re, S., Le Quere, B., Ghigo, J. M. and Beloin, C. (2007) Tight modulation of Escherichia coli bacterial biofilm formation through controlled expression of adhesion factors Appl Environ Microbiol.73:3391-403

Roux, A, Beloin, C. and Ghigo, J.M.(2005) A combined inactivation/expression strategy to study gene function in physiological conditions: application to the identification of new adhesins in E. coli. J. Bacteriol.187:1001-1013.

B. Lesic, S. Bach, J. M. Ghigo, U. Dobrindt, J. Hacker and E. Carniel. (2004.) Excision of the high-pathogenicity island of Yersinia pseudotuberculosis requires the combined actions of its cognate integrase and Hef, a new recombination directionality factor Mol Microbiol.52:1337-48.

A. Derbise, B. Lesic, D. Dacheux, J. M. Ghigo and E. Carniel. (2003.) A rapid and simple method for inactivating chromosomal genes in Yersinia FEMS Immunol Med Microbiol.38:113-6.

  M. K. Chaveroche, J. M. Ghigo and C. d'Enfert. (2000.) A rapid method for efficient gene replacement in the filamentous fungus Aspergillus nidulans Nucleic Acids Res.28:E97.

D. S. Weiss, J. C. Chen, J. M. Ghigo, D. Boyd and J. Beckwith. (1999.) Localization of FtsI (PBP3) to the septal ring requires its membrane anchor, the Z ring, FtsA, FtsQ, and FtsL J Bacteriol.181:508-20.

J. C. Chen, D. S. Weiss, J. M. Ghigo and J. Beckwith. (1999.) Septal localization of FtsQ, an essential cell division protein in Escherichia coli J Bacteriol.181:521-30.

 J. M. Ghigo, D. S. Weiss, J. C. Chen, J. C. Yarrow and J. Beckwith. (1999.) Localization of FtsL to the Escherichia coli septal ring Mol Microbiol.31:725-37.

J. M. Ghigo and J. Beckwith. (2000.) Cell division in Escherichia coli: role of FtsL domains in septal localization, function, and oligomerization J Bacteriol.182:116-29.

S. Letoffe, J. M. Ghigo and C. Wandersman. (1994.) Iron acquisition from heme and hemoglobin by a Serratia marcescens extracellular protein Proc Natl Acad Sci U S A.91:9876-80.

J. M. Ghigo, S. Letoffe and C. Wandersman. (1997.) A new type of hemophore-dependent heme acquisition system of Serratia marcescens reconstituted in Escherichia coli J Bacteriol.179:3572-9.

N. Wolff, P. Delepelaire, J. M. Ghigo and M. Delepierre. (1997.) Spectroscopic studies of the C-terminal secretion signal of the Serratia marcescens haem acquisition protein (HasA) in various membrane-mimetic environments Eur J Biochem.243:400-7.

A. Paquelin, J. M. Ghigo, S. Bertin and C. Wandersman. (2001.) Characterization of HasB, a Serratia marcescens TonB-like protein specifically involved in the haemophore-dependent haem acquisition system Mol Microbiol.42:995-1005.

M. S. Rossi, J. D. Fetherston, S. Letoffe, E. Carniel, R. D. Perry and J. M. Ghigo. (2001.) Identification and characterization of the hemophore-dependent heme acquisition system of Yersinia pestis Infect Immun.69:6707-17.

M. S. Rossi, A. Paquelin, J. M. Ghigo and C. Wandersman. (2003.) Haemophore-mediated signal transduction across the bacterial cell envelope in Serratia marcescens: the inducer and the transported substrate are different molecules Mol Microbiol.48:1467-80.

Biville, F.; Cwerman,  H; Rossi, M.S.; Drouet, V. Ghigo, J.M.  and C. Wandersman,(2004).The hemophore-mediated signal transduction system of Serratia marcescens: A new mode of regulation for an ECF sigma factor. Mol Microbiol. 53(4):1267-1277.

J. M. Ghigo and C. Wandersman. (1992.) A fourth metalloprotease gene in Erwinia chrysanthemi Res Microbiol.143:857-67.

J. M. Ghigo and C. Wandersman. (1992.) Cloning, nucleotide sequence and characterization of the gene encoding the Erwinia chrysanthemi B374 PrtA metalloprotease: a third metalloprotease secreted via a C-terminal secretion signal Mol Gen Genet.236:135-44.

S. Letoffe, J. M. Ghigo and C. Wandersman. (1993.) Identification of two components of the Serratia marcescens metalloprotease transporter: protease SM secretion in Escherichia coli is TolC dependent J Bacteriol.175:7321-8.

N. Wolff, J. M. Ghigo, P. Delepelaire, C. Wandersman and M. Delepierre. (1994.) C-terminal secretion signal of an Erwinia chrysanthemi protease secreted by a signal peptide-independent pathway: proton NMR and CD conformational studies in membrane-mimetic environments Biochemistry.33:6792-801.

S. Letoffe, J. M. Ghigo and C. Wandersman. (1994.) Secretion of the Serratia marcescens HasA protein by an ABC transporter J Bacteriol.176:5372-7.

J. M. Ghigo and C. Wandersman. (1994.) A carboxyl-terminal four-amino acid motif is required for secretion of the metalloprotease PrtG through the Erwinia chrysanthemi protease secretion pathway J Biol Chem.269:8979-85.

R. Binet, S. Letoffe, J. M. Ghigo, P. Delepelaire and C. Wandersman. (1997.) Protein secretion by Gram-negative bacterial ABC exporters--a review Gene.192:7-11.