EMBO practical course:
of biological macromolecules
by solution NMR
NMR has established itself as a powerful method for structure determination of biological macromolecules
in solution and is unique in extracting dynamical information on biological macromolecules over a large
range of timescales. The intention of the course is to provide practical training in current
state-of-the-art NMR techniques and computational methods for structure determination by NMR.
The emphasis will be on practical aspects, i.e. setting up experiments on the spectrometer (optimizing
parameters, pulse programs), processing and analyzing NMR data, and structure calculations.
The course is aimed at PhD students and postdocs who have some working knowledge of NMR theory,
i.e. product operator formalism, and who understand the basic principles of NMR pulse sequences.
Possible candidates should have the level of knowledge of students that have participated in a past
"Advanced NMR" EMBO lecture course on NMR theory organized by Kaptein, Oschkinat and Griesinger.
Martin Blackledge, Grenoble
Christian Griesinger, Frankfurt
Stephan Grzesiek, Basel
Peter Guentert, Zuerich
Ernest Laue, Cambridge
Maria Macias, EMBL Heidelberg
Lawrence McIntosh, Vancouver
Helen Mott, Cambridge
Michael Nilges, Paris
Michael Sattler, EMBL Heidelberg
Juergen Schleucher, Umea
Harald Schwalbe, Boston
Gert Vriend, Nijmegen
Geerten Vuister, Nijmegen
September 12-19, 2001
EMBL, Meyerhofstr. 1, D-69117 Heidelberg, Germany
Maps and instructions how to get to EMBL can be found
The course is planned for a maximum of 20 students.
Possible candidates should have the level of knowledge of students that have
participated in a past "Advanced NMR" EMBO lecture course on NMR theory
organized by Kaptein, Oschkinat and Griesinger.
Registration will only be possible on-line (see below). For registration (no fee),
applicants should describe their CV, NMR and research experience, current research and a
justification/motivation why they would like to participate.
Lodging will be available at the ISG hotel near EMBL (rooms shared by 2 people). Food and
lodging expenses will be covered by EMBO.
Application is not possible anymore. Successful applicants have been informed by e-mail.
We gratefully acknowledge support from Bruker, CIL, and Martek.
The course will start at 16:00 on Wednesday, Sept 12 and finish after lunch on Wednesday, Sept 19.
The course is intended to cover the experimental and computational aspects of
characterizing structure and dynamics of a biological macromolecules (proteins and
nucleic acids) by modern NMR techniques.
This includes setting up and understanding NMR experiments at the spectrometer,
triple resonance experiments, edited/filtered experiments, projection angle restraints
from residual dipolar couplings and from cross-correlated relaxation, hydrogen bonding,
heteronuclear relaxation. Software and methods for processing and analyzing NMR data
will be introduced. Structure calculations will be performed using DYANA and ARIA.
During a round table at the beginning of the course participants should introduce
themselves and briefly describe their current research.
Participants should bring a (small sized) poster about their research. The posters
will be mounted during the whole time of the course, and there will be a poster session
for presenting and discussing the posters.
Every subject will be introduced by lectures (1h each day).
The experiments and programs used during the practical will be demonstrated.
The remainder of the day will be devoted to practical work either on the
spectrometer, or on computers for analyzing NMR data and performing structure
The NMR practical will be organized in such a way that each participant (in
groups of 2-3) will be able to set up the experiments.
Data analysis, assignment and structure calculation will be performed by the
participants in small groups distributed throughout the week.
A sample project will be available for performing the NMR experiments, data
analysis, assignment and structure calculation. For each step, pre-recorded and
pre-processed data will be available.
We will try to make it possible for participants to bring their own data.
you can find the programme, teaching material and lecture notes from the previous
EMBO Practical Course which was held in Sept 1999.