Maps of the rRNA genes and intervening DNA of (A) B. burgdorferi and (B) B. garinii and B. afzelii. The B. burgdorferi map was derived from published data (Davidson et al., 1992; Schwartz et al., 1992; Gazuman et al., 1994). For the B. garinii and B. afzelii maps, sites were located as follows: SacII sites from the sequences of B. garinii G1 and B. afzelii R-1P3 16S rRNA (Marconi & Garon, 1992a and 1992b); HpaI sites, from Southern blotting data (Ojaimi et al., 1994) and the 16S sequences; EagI, BssHII and SmaI sites, from (Ojaimi et al., 1994) assuming identity with the B. burgdorferi 23S rRNA gene when assigning precise locations. Sites marked (Bg) or (Ba) were found only in B. garinii or B. afzelii, respectively. The SmaI site marked (Bg*) was present in B. garinii strains 20047, Ir210 and NBS16 but not in strains NT29, PD89 and Sika1. CO1, CO2, etc. indicate the location of PCR amplimers. The 16 kbp on the scales corresponds to map locations 458 kbp (B. burgdorferi), 469 kbp (B. garinii) and 474 kbp (B. garinii).