Physical & Genetic Maps

B. burgdorferi

• Borrelia burgdorferi 212 Physical map and coordinates
• Genetic map of Borrelia burgdorferi 212 (In preparation)

B. garinii

• Borrelia garinii 20047Physical map and coordinates
• Genetic map of Borrelia garinii 20047 (In preparation)

B. afzelii

• Borrelia afzelii VS461 Physical map and coordinates
• Genetic map of Borrelia afzelii VS461 (In preparation)

Comparisons

• Comparison of physical maps of B. burgdorferi 212 and Sh-2-82 (In preparation)
• Comparison of physical maps of B. burgdorferi 212, B. garinii 20047 and B. afzelii VS461 (In preparation)
• Comparison of genetic maps of B. burgdorferi 212, B. garinii 20047 and B. afzelii VS461 (In preparation)

Detailed maps

• Genetic map of dnaA region

• Genetic map of rrn region

  Maps of the rRNA genes and intervening DNA of (A) B. burgdorferi and (B) B. garinii and B. afzelii. The B. burgdorferi map was derived from published data (Davidson et al., 1992; Schwartz et al., 1992; Gazuman et al., 1994). For the B. garinii and B. afzelii maps, sites were located as follows: SacII sites from the sequences of B. garinii G1 and B. afzelii R-1P3 16S rRNA (Marconi & Garon, 1992a and 1992b); HpaI sites, from Southern blotting data (Ojaimi et al., 1994) and the 16S sequences; EagI, BssHII and SmaI sites, from (Ojaimi et al., 1994) assuming identity with the B. burgdorferi 23S rRNA gene when assigning precise locations. Sites marked (Bg) or (Ba) were found only in B. garinii or B. afzelii, respectively. The SmaI site marked (Bg*) was present in B. garinii strains 20047, Ir210 and NBS16 but not in strains NT29, PD89 and Sika1. CO1, CO2, etc. indicate the location of PCR amplimers. The 16 kbp on the scales corresponds to map locations 458 kbp (B. burgdorferi), 469 kbp (B. garinii) and 474 kbp (B. garinii).

References

1. Davidson, B. E., J. MacDougall and I. Saint Girons. 1992.
   Physical map of the linear chromosome of the bacterium Borrelia burgdorferi 212, a causative agent of Lyme disease, and localization of rRNA genes.
   J. Bacteriol. 174: 3766-3774.

2. Old, I. G., J. Macdougall, I. Saint Girons and B. E. Davidson. 1992.
   Mapping of genes on the linear chromosome of the bacterium Borrelia burgdorferi - possible locations for its origin of replication.
   FEMS Microbiol Lett. 99: 245-250.

3. Ojaimi, C., B. E. Davidson, I. Saint Girons and I. G. Old. 1994.
   Conservation of gene arrangement and an unusual organization of rRNA genes in the linear chromosomes of the Lyme disease spirochaetes Borrelia burgdorferi, B. garinii and B. afzelii.
   Microbiol. 140: 2931-2940.

4. Old, I.G., unpublished.

1. Casjens, S. and W. M. Huang. 1993.
   Linear chromosomal physical and genetic map of Borrelia burgdorferi, the Lyme disease agent.
   Mol. Microbiol. 8: 967-980.

2. Casjens, S., M. Delange, H. L. Ley, P. Rosa and W. M. Huang. 1995.
   Linear chromosomes of Lyme disease agent spirochetes: genetic diversity and conservation of gene order.
   J Bacteriol. 177: 2769-2780.