|Center for the Production and Infection of Anopheles|
|HEAD||BOURGOUIN Catherine, PhD / email@example.com|
|MEMBERS||Dr THIERY Isabelle (Engineer)/ BENALLAL Kamel (Master student)/ JACQUES Jean-Claude (Technicien superieur)/ LANDIER Annie ((Technicienne supérieure)/THOUVENOT Catherine (Technicienne)/ TOURON Solange (Technicienne)/ LECOQ Marie-Thérèse (Technicienne)/ BISSON Angélique (Trainee – Technicienne Supérieure)/GARNERO Sylvie (Secretary)
The CEPIA, CEnter for Production and Infection of Anopheles, is a platform which aim is to fulfilling the needs of several teams in Anopheles mosquitoes for diverse scientific programs in Malariology (Parasitology and Entomology). The basic concept was to concentrate in a single structure the production capacity and the competences for manipulating Anopheles mosquitoes.
As the ultimate goal of many projects in Malariology is to find ways to interrupt transmission of human malaria, the CEPIA has also capitalized in the production of Plasmodium falciparum gametocytes and of mosquitoes infected with this parasite species. These mosquitoes are used for studying Plasmodium-mosquito interactions and as a source of sporozoites that cannot be produced in vitro.
The objectives of the CEPIA are :
To ensure an intensive production of good quality mosquitoes, a quality system has been developed that includes standardized procedures for production and quality control tools, such as the weekly survey of the mosquito receptivity to a P. berghei GFP reference strain.
Since Avril 2006, routine infection of Anopheles with P. falciparum is being performed. This specificity permits to address several scientific issues pertaining to the transmission of the human malaria parasite including mosquito refractoriness and vaccine developments. Several collaborations, within and outside the Institute, have been initiated. In particular, we contribute to the functional genomic analysis of the An. gambiae APL1 locus, in collaboration with Dr K. Vernick (GGIV). This study demonstrated by gene-silencing assays that distinct genes of the APL1 gene family protect Anopheles gambiae against human and rodent malaria species. To increase research capacity for Anopheles–Plasmodium interaction studies, we are currently developing reliable procedures for in vitro production of infective P. falciparum ookinetes.
In the context of ecological survey of the effects of the Global Climate Changes, the CEPIA contributes with national and international teams to assess the competency of wild populations of European Anopheles species to P. falciparum.
Besides its ability to provide mosquito and parasite samples, the CEPIA is providing its expertise in Anopheles biology and functional genomics using RNA interference. As such, it interacts with several groups in France and Europe
Keywords: Plasmodium, Anopheles, sporozoite, oocyst, RNAi, malaria, interaction
Mitri, C., Jacques, J. C., Thiery, I., Riehle, M. M., Xu, J., Bischoff, E., Morlais, I., Nsango, S. E., Vernick, K. D., and Bourgouin, C. (2009a). Fine pathogen discrimination within the APL1 gene family protects Anopheles gambiae against human and rodent malaria species. PLoS Pathog 5, e1000576. PMID: 19750215
Mitri, C., Thiery, I., Bourgouin, C., and Paul, R. E. (2009b). Density-dependent impact of the human malaria parasite Plasmodium falciparum gametocyte sex ratio on mosquito infection rates. Proc Biol Sci 276, 3721-3726. PMID: 19656795
Emilie Pondeville*‡, Annick Maria*, Jean-Claude Jacques†, Catherine Bourgouin†, Chantal Dauphin-Villemant*‡ Anopheles gambiae males produce and transfer the vitellogenic steroid hormone 20-hydroxyecdysone to females during mating, PNAS, 105, 19631-19636. PMID: 19060216
Robert V, Bourgouin C, Depoix D, Thouvenot C, Lombard MN, Grellier P. Malaria and obesity: obese mice are resistant to cerebral malaria. Malar J. 2008 May 19;7:81. PMID: 18489748
Lambrechts, L., Morlais, I., Awono-Ambene, P. H., Cohuet, A., Simard, F., Jacques, J. C., Bourgouin, C., and Koella, J. C. (2007). Effect of infection by Plasmodium falciparum on the melanization immune response of Anopheles gambiae. Am J Trop Med Hyg 76, 475-480. PMID: 17360870
Activity Reports 2009 - Institut Pasteur
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