Enteric Bacterial Pathogens  


  HEADDr WEILL Francois-Xavier / francois-xavier.weill@pasteur.fr
  MEMBERSDr QUILICI Marie-Laure/ Dr LE HELLO Simon/ Dr FILLIOL Ingrid/ Dr PARDOS Maria/ Dr DOUBLET Benoit/ Dr ROBERT-PILLOT Annick/ GUIBOURDENCHE Martine/ LEFEVRE Martine/ GUIBERT Véronique/ ACCOU-DEMARTIN Marie/ CHAVIGNER-JOVE Brigitte/ LEJAY-COLLIN Monique/ CARLE Isabelle/ JOSSE Adeline/FABRE-BERLAND Laëtitia/ LEMEE Laure/ GUENOLE Alain/ RAUZIER Jean/ SONTAG Lucile/ BIDAULT Brigitte/PHALENTE Chrystelle/LANCASTRE Joëlle


  Annual Report

The Laboratory includes three National Reference Centers (CNR) and a WHO Collaborating Center. All modern aspects of bacterial biodiversity are considered with the purposes of designing new tools for molecular identification and typing of foodborne bacterial pathogens of concern.

National Reference Center for Salmonella. CNR-Salm contributes to the surveillance of salmonellosis in France by performing serotyping of 8-10,000 clinical isolates each year. CNR-Salm also performs fine molecular fingerprinting (PFGE, MLVA, MLST). CNR-Salm develops new subtyping methods (CRISPR polymorphism), and monitors antimicrobial resistance and the underlying molecular mechanisms. CNR-Salm has developed collaborations with prestigious teams working on population genetics and genome projects of serotypes Typhi and Paratyphi A, agents of typhoid and paratyphoid fevers.

National Reference Center for Escherichia coli-Shigella. CNR-coli participates in the surveillance of shigellosis and infections due to shigatoxin-producing E. coli (haemorrhagic colitis and haemolytic and uremic syndromes). E. coli strains are analyzed by serotyping (classical and molecular), PFGE and virulence gene content. CNR-coli develops new subtyping methods (CRISPR polymorphism, MLVA), and monitors antimicrobial resistance and the underlying molecular mechanisms.

National Reference Center for vibrios and cholera. CNR-Vibrio participates in the surveillance of cholera and non-cholera Vibrio infections in France and collaborates with foreign countries subject to cholera outbreaks and with humanitarian non-governmental organizations. Vibrio strains are identified by phenotypical and molecular (rpoB, rrs) methods, serotyping, and virulence gene content. CNR-Vibrio also performs molecular fingerprinting (PFGE and ribotyping). It is involved in control of food-borne and water-borne contamination by Vibrio species of medical interest and develops methods improving detection and quantification of pathogenic Vibrio strains.

WHO Collaborating Center for Salmonella. WHOCC for Salmonella maintains and updates the White-Kauffman-Le Minor scheme, which lists all known Salmonella antigenic formulae. Characterization of all serotypes by MLST and flagellin genes is ongoing.

Keywords: Salmonella, Shigella, Escherichia coli, Vibrio, Typhoid fever, dysentery, cholera, biodiversity, antimicrobial resistance

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  Publications

1: Fabre L, Delauné A, Espié E, Nygard K, Pardos M, Polomack L, Guesnier F, Galimand M, Lassen J, Weill FX. 2009. Chromosomal integration of the extended-spectrum beta-lactamase gene blaCTX-M-15 in Salmonella enterica serotype Concord isolates from internationally adopted children. Antimicrobial Agents and Chemotherapy. 53:1808-16. PubMed PMID :19273688.

2: Lizárraga-Partida ML, Quilici ML. Molecular analyses of Vibrio cholerae O1 clinical strains, including new nontoxigenic variants isolated in Mexico during the Cholera epidemic years between 1991 and 2000. 2009. Journal of Clinical Microbiology. 47:1364-71.PubMed PMID: 19213700.

3: Holt KE, Parkhill J, Mazzoni CJ, Roumagnac P, Weill FX, Goodhead I, Rance R, Baker S, Maskell DJ, Wain J, Dolecek C, Achtman M, Dougan G. 2008. High-throughput sequencing provides insights into genome variation and evolution in Salmonella Typhi. Nature Genetics. 40:987-93. PubMed PMID: 18660809.

4: Le TA, Fabre L, Roumagnac P, Grimont PA, Scavizzi MR, Weill FX. Clonal expansion and microevolution of quinolone-resistant Salmonella enterica serotype typhi in Vietnam from 1996 to 2004. 2007. Journal of Clinical Microbiology. 45:3485-92. PubMed PMID: 17728470.

5: Roumagnac P, Weill FX, Dolecek C, Baker S, Brisse S, Chinh NT, Le TA, Acosta CJ, Farrar J, Dougan G, Achtman M. Evolutionary history of Salmonella Typhi. 2006. Science. 314:1301-4. PubMed PMID: 17124322.





Activity Reports 2009 - Institut Pasteur
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