Proteomics - CNRS URA 2185  


  HEADDr NAMANE Abdelkader / abdelkader.namane@pasteur.fr
  MEMBERSCATINO Maria-Agata / Dr HEM Sonia / HOURDEL Véronique / LAURENT Christine / LENORMAND Pascal / Dr ROUSSELLE Jean-Claude / SAINT-MARTIN Françoise


  Annual Report

Proteomics is the study of the subsets of protein present in different parts of an organism and how they change with time and varying conditions.

Two-dimensional electrophoresis (2DE) currently remains the most popular separation technique for soluble proteins, and allows proteome mapping and comparison of protein expression under various conditions. We improved the sensitivity of detection by using fluorescent dyes. Gel-free proteomics techniques based on mono- or multidimensional liquid chromatography coupled with tandem mass spectrometry (MDLC-MSMS) complement our 2DE techniques. Two tandem mass spectrometers, the QSTAR XL, and the 4800 MALDI-TOF/TOF exist in the laboratory.

Numerous proteomics approaches were carried out in the laboratory. One main focus was the comparative analysis of relative protein expression under different growth conditions or of different strains of an organism. Comparative 2DE and stable isotope labelling of peptides (iTRAQ) followed by MDLC-ESI-MSMS were used for this aim.

"Targeted proteomics” involves initial protein enrichment by various methodologies. This approach was widely used for different projects, especially for protein-protein interactions and phosphoproteome analyses. For instance, affinity purifications of protein complexes using the TAP (Tandem Affinity Purification) approach resulted in the identification of partners involved in active protein complexes. Moreover, quantitative results obtained with SILAC labelling gave valuable information for the understanding of the protein order assembly within the pre-60s maturation process (collaboration with A Jacquier, Molecular Interaction Genetics). The TAP method is also applied to protein analyses of Helicobacter pylori collaboration with H de Reuse, Helicobacter Pathogenesis). After an enrichment step of phosphorylated proteins, a combination of 2DE, using specific staining and mass spectrometry analyses led to the identification of the Leishmania donovani phosphoproteome (collaboration with G Spaeth, Parasite Virulence).

In parallel to our scientific activity, which involves the Proteomics platform in many collaborative projects, we are also dedicated to service activities.

Keywords: Proteomics, electrophoresis, mass spectrometry, LC-MS/MS, proteins, tuberculosis, malaria

Ptprot.jpg

The 4800 MALDI-TOF/TOF



  Publications

  1. Wyers, F., Rougemaille, M., Badis, G., Rousselle, J. C., Dufour, M. E., Boulay, J., Regnault, B., Devaux, F., Namane, A., Seraphin, B., Libri, D., and Jacquier, A. (2005) Cryptic Pol II transcripts are degraded by a nuclear quality control pathway involving a new poly(A) polymerase. Cell 121: 725-737.

  2. Kisseleva-Romanova E, Lopreiato R, Baudin-Baillieu A, Rousselle JC, Ilan L, Hofmann K, Namane A, Mann C, Libri D.(2006) Yeast homolog of a cancer-testis antigen defines a new transcription complex.EMBO Journal 25(15):3576-85.

  3. Choumet V, Carmi-Leroy A, Laurent C, Lenormand P, Rousselle JC, Namane A, Roth C, Brey PT.(2007) The salivary glands and saliva of Anopheles gambiae as an essential step in the Plasmodium life cycle: a global proteomic study. Proteomics 7(18):3384-94

  4. Morales MA, Watanabe R, Laurent C, Lenormand P, Rousselle JC, Namane A, Späth GF. (2008) Phosphoproteomic analysis of Leishmania donovani pro- and amastigote stages. Proteomics 8(2): 350-63

  5. Stingl K, Schauer K, Ecobichon C, Labigne A, Lenormand P, Rousselle JC, Namane A, De Reuse H. (2008) In Vivo interactome of Helicobacter pylori urease revealed by tandem affinity purification. Molecular and Cellular Proteomics,7(12):2429-41



  Web Site

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Activity Reports 2009 - Institut Pasteur
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