Histotechnology and Pathology  


  HEADMichel-René Huerre , MD / mhuerre@pasteur.fr
  MEMBERSAlonso Françoise, secretary, full time/Avé Patrick, technician, full time/Bergere Sabrina, Technician, full time/Cardona Ana, DVM, PhD, full time/Chimy Marie Christine, technician, part time/Cumont Marie Christine, technician, full time/Fiette Laurence, DVM, PhD, full time/Huerre Michel-René, MD, full time/Jouvion Gregory, DVM, PhD, full time/Khun Huot, technician, full time/Le Gars Mathieu, student, DEA/Matondo Jeanne, laboratory assistant, part time/Maurin Sabine, technician, part time


  Annual Report

Our research programs in 2005-2008 are focused on the kinetics and patterns of inflammatory process in target organs in several models of infections. We routinely use a large panel of histological techniques combined with morphological tools including immunohistochemistry, in situ hybridisation, confocal, electronic microscopy and bioluminescence technology. Another goal of research is the study of neuroreceptors in using radio-imagers

Lung pathology: acute and chronic disease. Various pathogens can cause acute lung injury inducing the destruction of epithelial barriers, recruitment of inflammatory cells and then repair. Neisseria (Coll J.M. Alonso and M. Kheir) , Pseudomonas (Coll M. Chignard and Mustapha Si Taha), penetrate the bronchial tree in a few minutes or cross the pharynx (Bacillus anthracis Coll M. Mock & P. Goossens) inducing necrosis of bronchi and alveolae with a quick recruitment of Polymorphonuclear cells. Clostridium sordellii lethal toxin (coll. with B. Geny and M. Popoff) reproduce the fatal toxic shock syndrom observed in humans and animals with early extravasation of blood in the thoracic cavity without inflammatory cells. Virus (Coronavirus and Myxovirus; Coll P4 Lyon, P. Marianneau and N. Escriou, S. van der Werff) are able to destroy epithelial cells a few days after infections and to induce an inflammatory process followed by epithelial metaplasia. In using Bioluminescence and histology we demonstrated that Aspergillus fumigatus conidia germinate in the bronchoalveolar tree within a few hours of infection generating hyphae that cross the endothelia and invade the alveolar spaces. These patterns are observed in neutropenic patients and in animal models using vinblastin and corticosteroids.

Mucoviscidosis is studied in collaboration with the team of J.M. Sallenave, L. Touqui , M. Chignard and M. Legars in the unit in using the transgenic model CFTR..

Digestive pathology.

- Helicobacter pylori(Hp) induce ulcers and chronic gastritis un human pathology. We are currently carrying out a multicentre study with the international network of Pasteur Institute (Dakar, Antananarivo, Pnom Penh, Algiers, Noumea, Athens) The goal of this study is to assess the genetic diversity of Hp and to compare the pathology observed around the world. In parallel we study the patterns of the inflammatory reaction in transgenic mice especially the interferon pathways and IL-23 regulation (Coll. E. Touati) , the activation of Nf-kB ( Coll. R. Ferrero and S. Memet) and the pathogenicity of mutant strains (Coll H. de Reuse)

- Inflammatory Bowel Diseases is studied in using several models with the team of J.M.Sallenave. The role of elafin is under investigation.

Emerging viral infections

Study of the blood – brain barrier during HTLV- 1 infection (Coll S. Ozden, P.E. Ceccaldi and A. Gessain). Study of various type of Kaposi sarcoma, a model of malignant tranformation of Herpes virus VIII infected endothelial cells ) Study of emerging infections such as, Chikungunya, Coronavirus and Influenzae. Severe form of acute Chiungunya infection requiring intensive care unit have been investigated in human pathology with the physicians of La Reunion island especially atypical cases of encephalitis, nephritis, hepatitis, myocarditis (Photo)

Quantitative autoradiography based on the use of radioimagers (Beta-Imager and Micro-Imager: Ana Cardona)

Radioimagers provide an alternative to film autoradiography for in situ quantitative studies of tissue sections. They can detect tritium and the usual beta-emitting isotopes in electrophoresis gels or membranes, tissue sections on glass slides and whole body sections. Their major applications are: receptology( localisation and characterisation of isatin 5- hydroxyoxindole and acetyl choline recptors on brain sections) in situhybridisation (patterns of SLC25A12 expression during prenatal development) , in vivopharmacokinetics and the biodistribution of molecules (oxindole and indole compounds, increases in the urinary excretion of which have been linked to human neurodegenrative diseases).

Keywords: Infection, Inflammation, pathogenesis of infectious diseases, Radioimager, pharmacokinetic, neuroreceptors

Hispat.jpg

Legend: Chikungunya myocarditis. Chikungunya virus in myocardium. Streptavidin peroxidase & AEC x 200



  Publications

Glomski I.J., Huerre M.R.Mock M., Goossens P. Primary involvement of pharynx and Peyer’s patch in inhalational and intestinal anthrax. PLoS pathogen . 2007, 3(6): e76

Brock M., Jouvion G., Droin-Bergere S., Dussurget O., Nicola MA, Ibrahim-Granet O. Bioluminescent Aspergillus fumigatus, a new tool for drug efficiency testing and in vivo monitoring of invasive aspergillosis. Applied & Environmental Microbiol. 2008, 74 :7023-7035

Duprez R., Lacoste V., Briere J., Couppié P., Camilles F., Sainte Marie D., Kassa-Kehlembo E., Lando M.J., Essame-Oyono J.L., Nkegoun B., Hbid O., Mahé A., Huerre M.R., Gessain A. Evidence for a multiclonal origin of multicentric advanced lesions of Kaposi sarcoma. Journal of the National Cancer Institute, 2007, 99(14): 1086-1094

Afonso P., Ozden S., Cumont MC, Seilhan D, Payam R, Mason S, Lambert S, Huerre M, Gessain A, Couraud PO, Pique C, Ceccaldi PE, Romero IA. Alteration of blood barrier integrity by retroviral infection. Plos Pathogens, 2008,4:11:e1000205

Even N., Cardona A., Soudant M., Corringer P.J., Cloez-Tayarani I. Regional differential effects of chronic nicotine on brain alpha-4-containing and alpha-6 containing receptors. Neuroreport 2008,19(15):1545-1550





Activity Reports 2009 - Institut Pasteur
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