|Stems Cells and Development - CNRS URA 2578|
|HEAD||Dr. TAJBAKHSH Shahragim / firstname.lastname@example.org|
|MEMBERS||Dr. CASTEL David / Dr. CHRETIEN Fabrice / Dr. GAYRAUD-MOREL Barbara / Dr. LE ROUX Isabelle / Dr. MOURIKIS Philippos / Dr. SAMBASIVAN Ramkumar JORY Aurélie / MAIA Teresa / ROCHETEAU Pierre/ CROULLEBOIS Elisabeth / DUMAS Gérard / FLAMANT Patricia/ PAISANT Sylvain
Introduction: Our aim is to characterise stem
cells and their daughters during embryonic and
postnatal development of skeletal muscle to
understand how this tissue is established, and how it
regenerates during disease, and after injury. We are
examining the genetic networks which regulate
myogenic stem cell birth, and relating this to how
cell order is established in this lineage. We are
also investigating how stem/progenitor cells
self-renew, essentially via symmetric vs. asymmetric
cell divisions, and how the stem cell niche is
3)Regenerative myogenesis. Another aim is to isolate and characterise mouse and human stem cells and examine their function in reconstitution assays by transplantations into injured dystrophic or normal muscles. We are investigating the roles of Pax7 and the myogenic determination genes in this context. Using genetically modified mice and specific markers, we are examining the anatomy of the muscle niche and how this may regulate the stem cell state. Collectively, these studies should provide a link between the normal development of a tissue, its deregulation during disease, and its regeneration via the recruitment of stem and progenitor cells.
References: 1) Kassar-Duchossoy et al. (2005). Genes & Dev. 2) Tajbakhsh (2005) Exp. Cell. Res. 3) Shinin et al. (2006). Nature Cell Biology.
Keywords: stem cells, asymmetric cellular division, skeletal muscle, somites, satellite cells, Myf5, MyoD, Mrf4, Pax3, Pax7, Numb, “immortal” template DNA strands
Kassar-Duchossoy, L., Giacone, E., Gayraud-Morel, B., Jory, A., Gomès, D., Tajbakhsh, S. (2005). Pax3/Pax7 mark a novel population of primitive myogenic cells during development. Genes & Dev. 3:1426-1431.
Shinin, V., Gayraud-Morel, B., Gomes, D., and Tajbakhsh, S. (2006). Asymmetric division and cosegregation of template DNA strands in adult muscle satellite cells. Nat Cell Biol 8, 677-82.
Christov, C., F. Chretien, R.A. Khalil, G. Bassez, G. Vallet, F.J. Authier, Y. Bassaglia, V. Shinin, S. Tajbakhsh, B. Chazaud, and R.K. Gherardi (2007). Muscle Satellite Cells and Endothelial Cells: Close Neighbors and Privileged Partners. Mol Biol Cell. 18:1397-409. Epub 2007 Feb 7.
Gayraud-Morel, B., F. Chrétien, P. Flamant, D. Gomès, P.S. Zammit, and S. Tajbakhsh (2007). A role for the myogenic determination gene Myf5 in adult regenerative myogenesis. Dev Biol. 312:13-28 Epub 2007 Sep 11.
Cossu G, Tajbakhsh S. (2007). Oriented cell divisions and muscle satellite cell heterogeneity. Cell 129(5):859-61.
Activity Reports 2009 - Institut Pasteur
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