|Stems Cells and Development - CNRS URA 2578|
|HEAD||Dr. TAJBAKHSH Shahragim / email@example.com|
|MEMBERS||Dr. CHRETIEN Fabrice / Dr. GAYRAUD-MOREL Barbara / Dr. LE ROUX Isabelle / Dr. MOURIKIS Philippos / Dr. WINN Nadine / Dr. SAMBASIVAN Ramkumar
JORY Aurélie / MAIA Teresa / ROCHETEAU Pierre
Introduction: Our aim
is to characterise stem cells and their daughters during
embryonic and postnatal development of skeletal muscle to
understand how this tissue is established, and how it
regenerates during disease, and after injury. We are
examining the genetic networks which regulate myogenic
stem cell birth, and relating this to how cell order is
established in this lineage. We are also investigating
how stem/progenitor cells self-renew, essentially via
symmetric vs. asymmetric cell divisions, and how the stem
cell niche is defined.
3) Regenerative myogenesis. Another aim is to isolate and characterise mouse and human stem cells and examine their function in reconstitution assays by transplantations into injured dystrophic or normal muscles. We are investigating the roles of Pax7 and the myogenic determination genes in this context. Using genetically modified mice and specific markers, we are examining the anatomy of the muscle niche and how this may maintain the stem cell state. Collectively, these studies should provide a link between the normal development of a tissue, its deregulation during disease, and its regeneration via the recruitment of stem and progenitor cells.
References: 1) Kassar-Duchossoy et al. (2005). Genes & Dev. 2) Tajbakhsh (2005) Exp. Cell. Res. 3) Shinin et al. (2006). Nature Cell Biology.
Keywords: stem cells, asymmetric cellular division, skeletal muscle, somites, satellite cells, Myf5, MyoD, Mrf4, Pax3, Pax7, Numb, “immortal” template DNA strands
Kassar-Duchossoy, L., Gayraud-Morel, B., Gomès, D., Rocancourt, D., Buckingham, M., Shinin, V., Tajbakhsh, S. (2004). Mrf4 determines skeletal muscle identity in Myf5 :MyoD double mutant mice. Nature 431: 466-471.
Kassar-Duchossoy, L., Giacone, E., Gayraud-Morel, B., Jory, A., Gomès, D., Tajbakhsh, S. (2005). Pax3/Pax7 mark a novel population of primitive myogenic cells during development. Genes & Dev. 3:1426-1431.
Shinin, V., Gayraud-Morel, B., Gomes, D., and Tajbakhsh, S. (2006). Asymmetric division and cosegregation of template DNA strands in adult muscle satellite cells. Nat Cell Biol 8, 677-82.
Gayraud-Morel, B., F. Chrétien, P. Flamant, D. Gomès, P.S. Zammit, and S. Tajbakhsh (2007). A role for the myogenic determination gene Myf5 in adult regenerative myogenesis. Dev Biol. 312:13-28 Epub 2007 Sep 11.
Cossu G, Tajbakhsh S. (2007). Oriented cell divisions and muscle satellite cell heterogeneity. Cell 129(5):859-61.
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Activity Reports 2007 - Institut Pasteur
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