|Institut Pasteur Collection|
|HEAD||BIZET Chantal / firstname.lastname@example.org|
|MEMBERS||COUTELLIER Sabrina, secretary, email@example.com ANDRIAKOTO Nirina, secretary, firstname.lastname@example.org BIZET Chantal, Head of Unit, email@example.com
CAPDENAT Sophie, Master 2 in Microbiology BIMET François, engineer, firstname.lastname@example.org, CLERMONT Dominique, engineer, email@example.com CABANEL Nicolas, technician, firstname.lastname@example.org
GOMARD Marie-Thérèse, technician, email@example.com GUIBON Roselyne, technician, firstname.lastname@example.org (left in January 2007) GULAT-OKALLA Marie-Laure, technician, email@example.com HAMON Sylviane, technician, firstname.lastname@example.org
LOWENSKI Steeve, technician, email@example.com (left in May 2007) OUSSARGHIN Nadia, technician, firstname.lastname@example.org (maternity leave) PISSIS Cédric, technician, email@example.com
VIVIER Catherine, technician, firstname.lastname@example.org, ZOQUE Gustave, email@example.com
The role of the Collection de l’Institut Pasteur (CIP) is to ensure the maintenance and the enrichment of its bacterial collection, the diffusion of information on the distributed strains, the development of research on identification, taxonomy and preservation of bacteria. The CIP has been certificated ISO 9001 version 2000 on June 2001 and accredited for its molecular and biochemical methods since 2006. The CIP belongs to the Centre of Biological Resources of Institut Pasteur and participates to several international and national collection projects for the creation of the Biological Resources Centres.
1. Strain stock management
More than 9 000 strains are available on stock. Every year, new bacterial strains are added to the collection (about 300) coming either from researchers of the Institut Pasteur, or from foreign collections or from newly deposited strains after having connection with foreign or French researchers. The CIP contains also plasmids, transposons, host strains and probes. Strains are preserved in two different places and by two different ways (freeze dried and deep-frozen at - 80°C, or deep-frozen in nitrogen liquid and at - 80°C for strains which cannot be freeze-dried). In 2007, the CIP produced about 11 000 freeze dried samples and distributed about 4 500 strains. For some sensitive strains, L-drying has been tested.
2. Check up of the CIP strains
a) Total protein electrophoresis in discontinued gel
We are still working with information regarding protein profiles of more than 5000 strains, a databank is continuously set up.
b) Molecular characterization.
All the new incorporated strains are identified by sequencing the gene coding for 16Sr RNA. We begin to sequence this gene for all the strains of the collection. The sequencing of other genes like B, B, 65 is performed in relation with the genus studied.
3. Quality System
Process regarding activities and tasks are written in an organized process system which suits to the CIP organization. Tasks are recorded. Implemented processes are always followed up and improved. Regularly, internal audits are made in order to check and measure differences between what is written and what is done. Quality indices are provided with laboratory problems and requests from customers. If necessary, corrective actions are undertaken. Certification (ISO 9001) has been granted for 3 years. But, every year, a follow up audit must be made in order to keep it.
The CRBIP (including the CIP) has renewed its certification in 2007 according to the referential ISO 9001 version 2000 and the used methods by the CIP for identifying are accredited regarding 17025 standard since 2006.
4. Research activity
The description of a new specie of Pseudomonasin collaboration with E. Ivanova (Swinburne University of Technology, Australia) is under study. The reevaluation of the taxonomic position of Bacillus aeoliusis also under study in collaboration with the DSMZ (German collection of biological materials).
5. Sequencing of 16S rRNA gene for the type strains of the CIP
With the Genopole of the Institut Pasteur and in order to increase the enrichment of the CIP data base, the coding gene for 16S rRNA of the type strains is systematically sequenced.The sequence of 16S rRNA gene is obtained for more than 3 800 type strains.
Bacterial strains from external collections are preserved in the CIP. It’s submitted to a maintenance contract. Since 2002, this activity has been certifying with the standard ISO 9001 version 2000.
7. National relationships
The Ministry of Research created Biological Resources Centers (BRC) at a national level which is managed by a Committee of Biological Resources. The CIP belongs to this Committee. Its missions are to create a national network for BRC, to implement a Quality Insurance and to check the launch of quotations for creation and finance of accredited BRC,
9. International relationships
The CIP is a member of different official organizations : l’ECCO (European Culture Collections’ Organization – www.eccosite.org), EBRCN (European Biological Resource Centres Network – www.ebrcn.org), CABRI (Common Access to Biotechnological Resources and Information – www.cabri.org and WFCC (World Federation for Culture Collection).
In 1999, OECD (Organization for Economic Cooperation and Development) initialized an international meeting in Tokyo in order to look at the necessary conditions to set up Biological Resource Centers (BRC).
The Presidence of this project has been given to France and a study group is in charge of determining if it’s possible to create and implement biological resources centers. The CIP belongs to the French delegation and writes documents regarding the creation of the Insurance Quality implementation in collaboration with the other delegates.
Keywords: taxonomy, sequencing, biodiversity, collection, bacterial strains, studies, preservation, CBR
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Activity Reports 2007 - Institut Pasteur
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