Center for the Production and Infection of Anopheles  


  HEADBOURGOUIN Catherine, PhD / cabourg@pasteur.fr
  MEMBERSDr MITRI, Christian (Post doc)/ Dr THIERY Isabelle (Engineer)/ JACQUES Jean-Claude (Technicien superieur)
THOUVENOT Catherine (Technicienne)/ TOURON Solange (Technicienne)
LECOQ Marie-Thérèse (Technicienne)/ GARNERO Sylvie (Secrétaire)


  Annual Report

The CEPIA, CEnter for Production and nfection of Anopheles, has been created to fulfil the needs of several teams of the Institute in Anopheles mosquitoes for diverse scientific programs in Malariology (Parasitology and Entomology). The basic concept was to concentrate in a single structure the production capacity and the competences for manipulating Anopheles mosquitoes.

As the ultimate goal of many projects in Malariology is to find ways to interrupt transmission of human malaria, the CEPIA has also capitalized in the production of Plasmodium falciparum gametocytes and of mosquitoes infected with this parasite species. These mosquitoes are used for studying Plasmodium-mosquito interactions and as a source of sporozoites that cannot be produced in vitro.

The objectives of the CEPIA are :

  1. mass production of Anopheles mosquitoes that are natural hosts and vectors, or laboratory hosts for diverse Plasmodium species,

  2. production and maintenance of diverse Anopheles species or strains,

  3. providing lab facilities, equipment and expertise for the research teams in order to:

    1. perform mosquito infection with rodent malaria parasites,

    2. perform mosquito dissections or manipulations,

  4. producing P. falciparum gametocytes and performing Anopheles infection with this parasite species,

  5. set up functional genomic tools in Anopheles, such as transgenesis and RNAi.

To ensure an intensive production of good quality mosquitoes, a quality system has been developed that includes standardized procedures for production and quality control tools, such as the weekly survey of the mosquitoes receptivity to a P. berghei GFP reference strain.

Since Avril 2006, we have been successful at establishing the routine infection of Anopheles with P. falciparum. This achievement permits to address several scientific issues pertaining to the transmission of the human malaria parasite including mosquito refractoriness and vaccine developments. In particular, we contribute to the functional genomic analysis of the An. gambiae APL1 locus, in collaboration with Prof K. Vernick (GGIV).

The CEPIA is providing its expertise in molecular entomology for functional genomics in Anopheles using RNA interference. The plateform is also interacting with several groups in France and Europe providing mosquito samples and expertise.

Keywords: Plasmodium, Anopheles sporozoites, oocystes, RNAi, malaria, interaction

Cepia.jpg



  Publications

Briquet, S.; Boschet, C.; Gissot, M., Tissande, E., Sevilla, E., Franetich, J.-F., Thiery, I., Hamid, Z., Bourgouin, C., Vaquero, C., (2006) « The High Mobility Group Box (HMGB) nuclear factors of Plasmodium falciparum”, Euk. Cells, 5, 672-682, PMID: 16607015

Frischknecht, F., Martin, B., Thiery, I., Bourgouin, C. And Robert Menard (2006) “Using green fluorescent malaria parasites to screen for permissive vector mosquitoes”. Malaria J.5: 23, PMID: 16569221

Boisson, B., J. C. Jacques, V. Choumet, E. Martin, J. Xu, K. Vernick, and C. Bourgouin. 2006. Gene silencing in mosquito salivary glands by RNAi. FEBS Lett 580:1988-92. PMID: 16530187

Lambrechts L, Morlais I, Awono-ambene P., Cohuet A., Simard F., Jacques J.-C., Bourgouin C., and Koella J.(2007). Effect of infection by Plasmodium falciparum on the melanization immune response of Anopheles gambiae. American journal of Tropical Medicine and Hygiene, 78, 3, PMID: 17360870

Orlandi-Pradines E, Almeras L, Denis de Senneville L, Barbe S, Remoué F, Villard C, Cornelie S, Penhoat K, Pascual A, Bourgouin C, Fontenille D, Bonnet J, Corre-Catelin N, Reiter P, Pagés F, Laffite D, Boulanger D, Simondon F, Pradines B, Fusaï T, Rogier C.( 2007). Antibody response against saliva antigens of Anopheles gambiae and Aedes aegypti in travellers in tropical Africa. Microbes Infect.;9(12-13):1454-62., PMID: 17913537.





Activity Reports 2007 - Institut Pasteur
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