Electron Microscopy


  HEADPREVOST Marie-Christine / mprevost@pasteur
  MEMBERSDr BONNE Isabelle / Dr KERNEIS-GOLSTEYN Sophie / Dr SACHSE Martin


  Annual Report

The platform for electron microscopy (PFME) gives scientists of the Institute Pasteur the opportunity to utilize different high-resolution morphological approaches for their research needs. The PFME contributes to various projects across the whole campus. We also train new users to make best use of our equipment. The implantation of new techniques useful to study pathogen-host interaction is another part of our activity.

Main activities

Service

The PFME provides service for the morphological and immunohistochemistry analysis of pathogens of newly described diseases.

Collaborative research projects

Within these projects we provide morphological analysis by high resolution Scanning Electron Microscopy (SEM) or Transmission Electron Microscopy (TEM). We also perform different immunohistochemistry techniques from labeling before sample preparation for SEM and TEM to state of the art labeling on thawed cryo-sections, which offers good preservation of both, structure and epitopes.

Cryofixation is used for morphological studies or in combination with low temperature embedding in resin for immunohistochemistry. Examples for collaborative projects are morphological analysis of the biofilm of Aspergillus fumigatus, mutational screen of cell wall components of Mycobacterium tuberculosis, transmission of HTLV-1 virus.

Implantation of new technologies

We are currently establishing the conditions for Cryo- Electron Microscopy Of Vitrified Sections (CEMOVIS). This technique avoids the artifacts of chemical fixation and allows the study of a sample as close to the native state as possible because the hydrated sample is analyzed without any staining of heavy metals.

Improved morphology of the periplasmic space by cryo-fixation. Shigella flexneri was fixed by high pressure freezing, followed by freeze substitution and embedding in EPON. The insert shows a detail of the periplasmic space with the outer membrane (black arrow), the inner membrane (white arrow) and the peptidoglycan layer (black arrowhead). Bar = 50 nm (Work with the laboratory of Prof. Sansonetti).

prevost.jpg



  Publications

Publications 2006 of the unit on Pasteur's references database




Activity Reports 2006 - Institut Pasteur
If you have problems with this Web page, please write to rescom@pasteur.fr