Macromolecular Interaction Genetics - CNRS URA2171  

  MEMBERSDr FROMONT-RACINE Micheline / HANTRAYE Florence / Dr NEIL Helen / Dr SAVEANU Cosmin

  Annual Report

Budding yeast, the first eukaryote whose genome was entirely sequenced, is the organism of reference for functional genomic studies. It is today a unique model to study cellular functions in eukaryotic cells. Historically, our laboratory had a strong expertise in the identification of two-hybrid interaction networks. We have now implemented and developed other innovative functional genomic approaches that we apply to uncover novel fundamental aspects of RNA metabolism following two main themes.

Ribosome biogenesis

Ribosomes paragraphare very abundant protein factories. Their synthesis can consume up to 80% of the metabolic energy in proliferating cells. It is thus not surprising that ribosome synthesis is connected with other essential cellular processes such as translation initiation or cell cycle progression.

During the last six years, it was discovered that, in eukaryotes, and in contrast to eubacteria, an unexpectedly large number of factors (up to 200) are required for ribosome biogenesis. These factors are highly conserved among eukaryotes. We contributed to the identification of many of these new factors, began to establish functional interaction networks and described the order of assembly and dissociation of several proteins within the large pre-ribosomal complexes. A future challenge is to understand both the mechanistic details of the ribosome assembly process as well as the molecular bases of the identified genetic interactions between ribosome biogenesis and other major cellular pathways.

RNA degradation, regulation and quality control

Regulation of gene expression can be exerted at any step, including RNA maturation, nucleo-cytoplasmic export, degradation and translation, to talk only of the steps involving RNA. Regulations can be efficient only because RNAs (as well as proteins) are submitted to an active degradation process that enables their turnover. Several general degradation pathways recognize and degrade in controlled manners “normal” transcripts. These degradation processes can be directly regulated. In addition, there are RNA quality control processes that recognize and target non-functional transcripts for degradation. These controlled/regulated RNA degradation processes and RNA quality-control mechanisms are highly intermingled, sharing a number of factors. Since several years, we study these mechanisms that turn out to play central roles in controlling the expression of the genetic information.



Publications 2006 of the unit on Pasteur's references database

Activity Reports 2006 - Institut Pasteur
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