Biodiversity of Emerging Pathogenic Bacteria

  HEADProf. GRIMONT Patrick /
  MEMBERSDr BREZILLON Christophe / Dr BRISSE Sylvain / LEFLECHE Anne / Dr FILLIOL Ingrid
GUIBOURDENCHE Martine / Dr GUTIERREZ Maria Cristina / WEILL Francois-Xavier

  Annual Report

The Unit includes research laboratories, a WHO Collaborating Center, three National Reference Centers (CNR), and a Center for Molecular Identification of Bacteria. All modern aspects of bacterial taxonomy are considered with the purposes of defining bacterial species and designing new tools for molecular identification and typing.

Molecular Taxonomy and Population Genetics. Our basic research encompasses the quest for the bacterial species concept. Quantitative DNA-DNA hybridization, rrs and rpoB gene sequencing, ribotyping and other methods are used to delineate bacterial species, as recently applied to the genera Pseudomonas, Burkholderia or Proteus. We study bacterial strain diversity and evolution in selected pathogenic species such as Salmonella (including serotype Typhi, the agent of typhoid fever), Escherichia coli, Mycobacterium, Klebsiella and Acinetobacter.

WHO Collaborating Center for Salmonella. WHOCC for Salmonella maintains and updates the White-Kauffman-Le Minor scheme, which lists all known Salmonella antigenic formulae. Characterization of serotypes by multilocus sequence typing and flagellin gene is ongoing.

National Reference Center for Salmonella. CNR-Salm contributes to the surveillance of salmonellosis in France by performing serotyping of 10,000 clinical isolates each year. CNR-Salm also performs fine molecular fingerprinting e.g. for serotypes Typhi, Typhimurium, Paratyphi B and Enteritidis. CNR-Salm develops new subtyping methods, and monitors antimicrobial resistance and the underlying molecular mechanisms.

National Reference Center for Escherichia coli-Shigella. CNR-coli participates in the surveillance of shigellosis and infections due to shigatoxin-producing E. coli (haemorrhagic colitis and haemolytic and uremic syndromes). E. coli strains are analyzed by serotyping (classical and molecular), ribotyping and virulence gene content.

National Reference Center for Corynebacterium diphtheriae. CNR-Cd identifies C. diphtheriae and detects diphtheria toxin genes. It maintains a ribotype database for WHO.

Center for Molecular Identification of Bacteria. CMIB identifies by sequencing strains from clinical, veterinary, environmental, or industrial sources. Strains that do not fit any described species are included in our current taxonomic research.



Publications 2006 of the unit on Pasteur's references database

Activity Reports 2006 - Institut Pasteur
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