Mouse Genetics Engineering Center


  HEADDr. LANGA VIVES Francina / francina@pasteur.fr
  MEMBERSBEAU Rémi / GUINUT Frédéric / THOURON Françoise


  Annual Report

The Centre d’Ingénierie Génétique Murine (CIGM) is a technological core facility created in 2003, which aims to generate new models of genetically-engineered mice either by classical transgenesis or by homologous recombination in embryonic stem (ES) cells (targeted transgenesis). The services of the CIGM are open preferentially to the Institut Pasteur scientific community, but also to external customers interested in new transgenic, knock-out (KO) or knock-in (KI) mouse models. The core facility also provides expert advice regarding the design of gene constructs suitable for transgenesis and homologous recombination experiments.

The classical transgenesis service, involving the microinjection of the gene of interest into the pronuclei of fertilized eggs, was originally the main activity of the CIGM. The microinjected transgenes include DNA fragments, BACs and YACs. The genetic backgrounds proposed include hybrids, such as B6SJLF1, B6D2F1 or B6CBAF1, as well as pure genetic backgrounds, such as C57BL/6, BALB/c or FVB. Classical transgenesis activity increased considerably in 2006, particularly BAC microinjections and use of the C57BL/6 genetic background, the model of choice for studies in neuroscience and immunology. Moreover, this year we developed the technique of transgenesis by lentiviral constructs in order to increase the efficiency of transgene insertion into the mouse genome. Our results demonstrate, and corroborate reports in the literature, that subzonal injection of fertilized eggs with viral particles containing the transgene of interest is more efficient than pronuclear injection.

The homologous recombination technique in ES cells and the subsequent microinjection of these modified ES cells into mouse blastocysts allow the generation of germ-line chimeras for targeted transgenesis in the endogenous gene. We obtained our first germ-line chimera (Fig 1) in June 2004 and, since then, we have generated 22 heterozygous/homozygous mice lines corresponding to new KO or KI mouse models.

Since November 2005, the CIGM disposes of a new independent animal room totally equipped with ventilated racks. This new animal room has increased the mouse housing capacity by 45% and also the quality of the health status of the mice generated at the CIGM.

Since its creation, the CIGM interacts with an expanding list composed of 25 Units at the Institut Pasteur (belonging to 7 out of the 10 new Departments), and other Research Institutions in France (CNRS Unit UMR 7622-Paris UMR146-Institut Curie-Orsay, INSERM Units E-365, U787 and E9935-Paris, and U522-Rennes, the Institut National de la Transfusion Sanguine-Paris) and also in foreign countries (Université Libre de Bruxelles- Belgium, Universitat de Barcelona-Spain).

Finally, the CIGM is committed to training activities, and in 2006 we undertook a program of training for the future head of the homologous core facility at the Institut Pasteur de Montevideo (Uruguay).

langa-vives.jpg

Fig 1. Sibelius, première germ-line chimère générée au CIGM.

Fig 1. Sibelius, first germ-line chimera generated at the CIGM



  Publications

Publications 2006 of the unit on Pasteur's references database




Activity Reports 2006 - Institut Pasteur
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