Center for the Production and Infection of Anopheles


  HEADDr BOURGOUIN Catherine / cabourg@pasteur.fr
  MEMBERSDr MITRI Christian / Dr THIERY Isabelle


  Annual Report

The CEPIA, CEnter for Production and Infection of Anopheles, was created to fulfill the needs of several teams of the Institute in Anopheles mosquitoes for diverse scientific programs in Malariology (Parasitology and Entomology). The basic concept was to concentrate in a single structure the production capacity and the competences for manipulating Anopheles mosquitoes.

As the ultimate goal of many projects in Malariology is to find ways to interrupt transmission of human malaria, we also proposed to include as a specific competence of the CEPIA the production of Plasmodium falciparum gametocytes and of mosquitoes infected with this parasite species. These mosquitoes are used for studying Plasmodium-mosquito interactions and as a source of sporozoites that cannot be produced in vitro.

The objectives of the CEPIA are:

  1. mass production of Anopheles mosquitoes that are natural hosts and vectors, or laboratory hosts for diverse Plasmodium species,

  2. production and maintenance of diverse Anopheles species or strains,

  3. providing lab facilities, equipment and expertise for the research teams in order to:

  4. perform mosquito infection with rodent malaria parasites,

  5. perform mosquito dissections or manipulations,

  6. producing P. falciparum gametocytes and performing Anopheles infection with this parasite species,

  7. set up functional genomic tools in Anopheles, such as transgenesis and RNAi.

To ensure a high production of good quality mosquitoes, a quality system has been developed that includes standardized procedures for production and quality control tools, such as the survey of the receptivity of mosquitoes to a P. berghei GFP reference strain.

Since April 2006, we have been successful at establishing the routine infection of Anopheles with P. falciparum. This achievement will now permit to address several scientific issues that were usually addressed using rodent plasmodium models.

The CEPIA is also providing its expertise in molecular entomology for functional genomics in Anopheles using RNA interference.

bourgouin.jpg

Mature male and female gametocytes



  Publications

Publications 2006 of the unit on Pasteur's references database




Activity Reports 2006 - Institut Pasteur
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