Unit: Fungi Culture Collection
Director: Bernard PAPIEROK
The Collection des Champignons of the Institut Pasteur is in charge of about 2500 strains of fungi (filamentous fungi and yeasts) and Actinomycetes, most of which originated from human lesions. As an integral part of the Centre de Ressources Biologiques de l'Institut Pasteur, this collection was given in July 2005 certification according to the international standard ISO 9001 : 2000. In addition to specific collection activities (culture, conservation, identity and viability checking, distribution), the laboratory carried out investigations on systematics of arthropod-pathogenic Entomophthorales.
The Collection des Champignons (Fungal Culture Collection) of the Institut Pasteur is in charge of about 2500 strains of fungi (filamentous fungi and yeasts) and Actinomycetes, most of which originated from human lesions. This collection includes also strains from environmental origin (air, soil, decaying plant material, dead invertebrates, mostly insects). To keep the collection is the basic purpose of the laboratory. In addition, research investigations are carried out on original isolates.
The laboratory is keeping strains in pure culture and delivers them to the interested research laboratories, as well as to industrial and pharmaceutical ones and to education establishments. About 600 strains were distributed in 2005. Furthermore, strain identification of contaminants (from industrial process, environment ) is performed on request. The laboratory provided also a large part of the isolates used in the laboratory work at the annual Institut Pasteur Medical Mycology course. It provided in 2005 fungal cultures to the Medical Mycology course of the Institute of Infectiology in Bern (Switzerland).
The Collection des Champignons is registered under the number 344 (acronym: UMIP) in the World Directory of Collections of Cultures of Micro-organisms (WDCM).
Quality control and certification
As an integral part of the Centre de Ressources Biologiques de l'Institut Pasteur (Biological Resource Centre of the Institut Pasteur), the Collection des Champignons intensively developed an organization and quality control strategy to meet the requirements of the international standard ISO 9001 : 2000. The reward of these efforts was the certification officially delivered in July 2005. The involved activities cover accession, production, preservation and distribution of biological materials.
The Collection des Champignons has then undertaken a programme to become accredited according to the ISO 17025 standard.
Systematics and host specifity of Entomophthorales
In June 2004, a peculiar entomophthoralean species was found on larvae as well as adults of a chrysomelid species (Coleoptera) in Poland (collaboration with Cezary TKACZUK, Department of Plant Protection, University of Podlasie, Siedlce, Poland). A special attention was paid to this species of the genus Zoophthora, given most species of this genus are infecting Hemiptera and Lepidoptera. Interestingly, the Polish material is being compared to a Zoophthora material found on another species of Coleoptera (on larvae only) in France in May 2005. These investigations should allow a better understanding of some host specificity aspects within an ecologically important group of fungal entomopathogens.
Keywords: Mycology, Strain collection, Quality control, BRC, Entomophthorales, Systematics