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     Cytometry


  Director : Philippe METEZEAU (metezeau@pasteur.fr)


  abstract

 

The laboratory makes flow cytometry analysis and sorting. It organizes learning and development of methodologies and technologies in the field of cell analysis and sorting.



  report

cale

The laboratory is primarly useful for experiments in the field of flow and image cytometry.

The principal activities are cell analysis and sorting. We use a Laser scanning image cytometer, a confocal microscope, three cell-sorters, and four flow cytometrer-analysers. Particularly, we have obtained a very rapid cell sorter (25 000 cell analyzed and sorted /sec.) ; it is a good example of second generation of practical sorter. Lasers which equiped our machines allow visible and UV excitations of many fluorochromes (for example, new fluorochromes : PE-Cy5 and 7) . 5 to 7 fluorescent signal can be detected simultaneously. The most demanding applications are immunolfluorescent detections, cell cycle analysis., apoptosis and pH measurements (5000 analysis and 350 sortings by year). Moreover, the laboratory organizes learning (85 students)

Keywords: flow cytometry, confocal microscopy, cell sorting, celle analysis, image cytometry, fluorescence



  publications

puce Publications 2003 of the unit on Pasteur's references database


  personnel

  Office staff Researchers Scientific trainees Other personnel
        BALAZUC Anne Marie, Ingeneer abalazuc@pasteur.fr

LOUISE Anne , ingeneer alouise@pasteur.fr

KIEFER BIASIZZO Hélène, technician kiefer@pasteur.fr

WAGNER Marie Christine, technician, mcwagner@pasteur.fr

Activity Reports 2003 - Institut Pasteur
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