|Mouse Genetics Engineering Center|
|Director : Francina Langa Vives (email@example.com)|
Officially created in June 2003, the Centre d'Ingénierie Génétique Murine (CIGM) is a novel technological facility at Institut Pasteur. This service aims to generate genetically-engineered mice either by classical transgenesis techniques or by homologous recombination in embryonic stem (ES) cells. The CIGM is open to the Pasteur community and also to external customers interested in new transgenic, knock-out or knock-in murine models.
The Centre d'Ingénierie Génétique Murine (CIGM) has been officially created in June 2003. The CIGM is a novel technological facility at Institut Pasteur, attached to the Département de Biologie du Développement. The CIGM aims to generate new models of genetically-engineered mice by classical transgenesis techniques or by homologous recombination in embryonic stem (ES) cells (targeted transgenesis).
At present, the CIGM takes up a 54-m2 surface in the ground floor of Fernbach building, including a microinjection laboratory and a cell culture laboratory. In addition, the CIGM has a room in the animal house of Monod building. The main equipment of CIGM consists of 2 microinjection sets (including a microinjection station ASTP Leica-Eppendorf constituted by an inverted microscope Leica DMIRE2 with 2 Eppendorf Transferman NK2 micromanipulators), 4 stereomicroscopes Nikon SMZ800, 2 routine inverted microscopes Olympus CK40, 3 CO2 incubators MCO 17AIC, 2 laminar flow cabins Holten Safe 2010 and 1 electroporator Bio-Rad Gene Pulser X Cell Total System.
In 2003, the main activity of CIGM has been classical transgenesis, consisting in the microinjection of the gene of interest into the pronuclei of fertilized eggs. We have carried out 15 collaborations, and more than 50 "transgenic founder" mice have been obtained. Our collaborators include 7 Units from Institut Pasteur, external customers such as the Institut National de la Transfusion Sanguine, the INSERM Units E9935 of Paris and U522 of Rennes, the Université Libre de Bruxelles and also the private start-up Cellectis.
The homologous recombination technique in ES cells and subsequent microinjection of modified ES cells in mouse blastocysts allow the generation of germ line chimeras for targeted transgenesis in the endogenous gene. This activity has started at CIGM in June 2003. Since then, we have initiated 4 collaborations with different Units from Institut Pasteur, and 7 projects have been evaluated and they are in waiting list for 2004 (the construction of the homologous recombination vectors is ongoing in the respective laboratories).
Our service also provides expertise for the design of gene constructions suitable for the transgenesis and/or homologous recombination experiments.
Keywords: mouse, transgenesis, homologous recombination, Embryonic Stem (ES) cells, microinjection
|Publications 2003 of the unit on Pasteur's references database|
|Office staff||Researchers||Scientific trainees||Other personnel|
|LANGA VIVES, Francina PhD Head of service, firstname.lastname@example.org
RUDINGER, Stéphanie (CDD) Technician, email@example.com
THOURON, Françoise Technician, firstname.lastname@example.org