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  Director : Christiane Bouchier (bouchier@pasteur.fr)


  abstract

 

The Genomics platform carries out large-scale projects with several teams of Pasteur Institute as part of the Genopole programme on Microbiology initiated by the French Ministry of Education and Research. Following a third call for proposals issued by the Genopole Institut Pasteur, genomic projects were selected by a steering committee in November 2002. These projects are being carried out following an automation-based protocol providing a high-thoughput low-cost method for the isolation and purification of plasmid template samples for use on capillary-based DNA sequencing devices. The Genomics platform has 5 capillary sequencers (ABI model) and a robotic workstation (Genesis, Tecan).



  report

cale

List of projects selected by the Steering committee and carried out between 2000 and 2002.

Update : December 2002

 

Manager

Nber of Purification of cloned DNA

Nber of sequences

Status

Complete genomic sequence of Candida glabrata (13 Mb)

B. Dujon

76 320

150 336

finishing in progress

Complete genomic sequence of Microcystis aeruginosa (4,6 Mb)

N. Tandeau de Marsac

38 850

77 123

shotgun in progress

Comparative Genomic - Tuberculosis complex

K. Eiglmeier - T. Garnier

9 792

19 584

finished

Partial genome sequencing of Mycobacterium ulcerans

T. Stinear

27 840

55 680

finished

EST sequencing of Aspergillus nidulans

C. d'Enfert

4 512

9 024

finished

PCR products sequencing - ORF of Helicobacter pylori

A. Labigne

-

1 827

finished

Molecular evolution of snake venom enzymes

V. Choumet

4 512

9 024

finished

EST sequencing of Entamoeba histolytica

N. Guillen

3 744

4 608

finished

EST sequencing of Plasmodium yoelli

G. Snounou

3 072

3 072

finished

EST sequencing of Theileria annulata

G. Langsley

-

960

ongoing

Allelic diversity of Plasmodium falciparum

O. Puijalon

-

6 784

ongoing

Multilocus Sequence Typing of Candida albicans strains

C. d'Enfert

1728 PCR products

3456

ongoing

Molecular typing of CIP Bacteria strains - 16S rRNA gene

C. Bizet

432 PCR products

3 660

ongoing

Photo: the team during the annual seminar of the Structure and Dynamic of Genomes Department in June 2002.

Keywords: High-throughput sequencing, Genome, Sequence analysis



  web site

puce More informations on our web site


  publications

puce Publications of the unit on Pasteur's references database


  personnel

  Office staff Researchers Scientific trainees Other personnel
        Ma Laurence technician lauma@pasteur.fr

Magnier Arnaud technician amagnier@pasteur.fr

Motreff Laurence technician lmotreff@pasteur.fr

Ochoa Eliana technician (short-term contract) eochoa@pasteur.fr

Tichit Magali technician mtichit@pasteur.fr

Zidane Nora technician (short-term contract) nzidane@pasteur.fr


Activity Reports 2002 - Institut Pasteur
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