|PDF Version||Protein Microsequencing and Analysis|
|Director : d’Alayer Jacques (email@example.com)|
During 2002 the Protein Microsequencing and Analysis Technical Platform continued its activity of protein microsequencing for the benefit of laboratories both inside and outside the Institut Pasteur.
The service continued to analyze proteins using a new technology called "ProteinChip Technology" (Ciphergen). This technology combines the capture of proteins on different chemical surfaces and their analysis by mass spectrometry (MALDI-TOF).
Since 2001, the laboratory has changed its name and has been assigned to Director of Strategic Equipment and Technologies.
Its principal activity continued to be protein microsequencing. Amino acid sequences are determined from purified or partially purified proteins and peptides. We can also obtain internal sequences by cutting proteins into fragments and purifying them by HPLC chromatography before sequencing. The amino acid sequence is determined automatically by machines using the Edman degradation technique ( two sequencers: an Applied Biosystems 473A and a 494 are available). A service of support and development for the microtechniques needed to prepare samples for analysis is also provided.
During 2002, 77 units or laboratories, including 53 from outside the institute, have used our protein microsequencing services (>5800 sequencing cycles were run).
The analysis of proteins by mass spectrometry after capture on various chemical surfaces (ProteinChip Arrays) began to be an important activity of our Technical Platform. Because of the diversity of ProteinChip surfaces, ( http://www.ciphergen.com/ ) a large number of diverse analyses can be carried out: specific molecular interaction studies (receptor/ligand, protein/protein or DNA/protein), differential protein expression studies (discovery and validation of biological markers) and the purification, identification and characterization of proteins. These analyses can be carried out with the very small amounts of materials needed for mass spectrometry.
During 2002, 22 units or laboratories, including 6 from outside the institute, have used our protein analysis services.
The Technical Platform participated in training and seminars both on and off the campus
Keywords: proteins, microsequencing, ProteinChips, mass spectrometry
|Publications of the unit on Pasteur's references database|
|Office staff||Researchers||Scientific trainees||Other personnel|
|d’Alayer, Jacques,( Engineer,firstname.lastname@example.org)
Chrisment, Peggy, (Engineer, from aug. 2001 to aug. 2002)
Brès, Evelyne, (Engineer, since nov. 2002,email@example.com)