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  Director : BIZET Chantal (bizet@pasteur.fr)


  abstract

 

The role of the Collection de l'Institut Pasteur (CIP) is to ensure the maintenance and the enrichment of its bacterial collection, the production of a catalogue (the 7th edition was published in 1998), the diffusion of information on the distributed strains and finally the development of research on identification, taxonomy and preservation of bacteria. The CIP is the first French ISO 9001 certificated collection and participates to several international and national projects for the creation of the Biological Resources Centres.



  report

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1. Strain stock management

7.500 strains are available on stock. Every year, new bacterial strains are added to the collection (about 400) coming either from researchers of the Institut Pasteur, or from foreign collections or from newly deposited strains after having connection with foreign or French researchers. We've got also plasmids, transposons, host strains, probes. Titrated spore suspensions (species Bacillus and Clostridium) are available. Strains are preserved in two different places and by two different ways (freeze dried and deep-frozen at - 80°C, or deep-frozen in nitrogen liquid and at - 80°C for strains which can't be freeze-dried).

2. Check up of the CIP strains

a) Total protein electrophoresis and chimiotaxonomy

This laboratory helps in developing bacterial identification methods by analysis of bacterial cell wall components : lipids, peptidoglycane, sugars... and total proteins. Main techniques used are chromatography

and derivated techniques and also electrophoresis in discontinued gel. Results are used thanks to a

computerized process with specific softwares.

We are still working on feeding several databanks with information regarding protein profiles and nature of cell wall components.

b) A database with 800 profiles obtained after ribotyping has been performed.

This laboratory is necessary for the Collection strain check and strain expertise for industrial laboratories

3. Quality System

Process regarding activities and tasks are written in an organized process system which suits to the CIP organization. Tasks are recorded. Implemented processes are always followed up and improved. Regularly, internal audits are made in order to check and measure differences between what is written and what is done. Quality indices are provided with laboratory problems and requests from customers. If necessary, corrective actions are undertaken. Certification (ISO 9001) has been granted for 3 years. But, every year, a follow up audit must be made in order to keep it.

The CIP has renewed its certification in July 2002 according to the new referential ISO 9001 version 2000.

4. Re-identification of bacterial strains after description of new species

In 2002, Corynebacterium strains have been studied by whole cell protein electrophoresis and by rDNA sequencing. Their phenotypic characteristisation was also performed.

The results obtained allowed to identify many of the strains studied and help to find a new species.

5. Gene sequencing of rRNA 16S for bacterial type strains of the Collection

Within the framework of a proposal submission and in order to contribute to the enrichment of the database of the CIP, the gene coding for the 16S rRNA of type strains is sequenced.

6. Studies

The CIP organizes quality controls for public or private laboratories. An external quality control was organized thanks to a partnership with the Comité de Lutte contre les Infections Nosocomiales located in the Centre of France (CLIN). 21 laboratories received 6 multiresistant strains for testing. Bacterial strains from external collections are preserved in the CIP. It's submitted to a maintenance contract. CIP provides industrial laboratories with spore suspensions.

This activity is going to be submitted to Quality Insurance.

7. National relationships

The Ministry of Research created Biological Resources Centers (BRC) at a national level which is managed by a Committee of Biological Resources. The CIP belongs to this Committee. Its missions are

to create a national network for BRC and to implement a Quality Insurance and to check the launch of quotations for creation and finance of accredited BRC,

8. International relationships

The CIP is a member of different official organizations : l'ECCO (European Culture Collections' Organization - www.eccosite.org), CABRI (Common Access to Biotechnological Resources and Information - www.cabri.org and WFCC (World Federation for Culture Collection).

1. In 1999, OECD (Organization for Economic Cooperation and Development) initialized an international meeting in Tokyo in order to look at the necessary conditions to set up Biological Resource Centers (BRC). It is requested that :

The Presidence has been given to France and a study group is in charge of determining if it's possible to create and implement biological resources centers. The CIP belongs to the French delegation and helps in writing documents regarding the creation of the Insurance Quality implementation.

2. European Biological Resource Centers Network (EBRCN)

The CIP participates to EBRCN.

This European project enables Europe to :

. show its study skills in Biological Resource Centers (CRB) in computing

. create global policy

. maintain a high level of quality for the services provided by European Collections.

Aims of this project are the following :

. to create a Biological Resource Centers network including alive organisms, components and associated data bases, and to answer OECD new decisions in an international context.

. to develop European standards for BRC, based upon management quality system

. to create a framework in order to increase complementarity and to decrease redundancy among European BRC.

. to introduce new techniques in Information Technology in order to bring added value to the catalogue and to give a better access.

. to get and provide information on BRC.

Keywords: taxonomy, sequencing, biodiversity, collection, bacterial strains, studies, preservation



  web site

puce More informations on our web site


  publications

puce Publications of the unit on Pasteur's references database


  personnel

  Office staff Researchers Scientific trainees Other personnel
  BIZET Chantal, curator,bizet@pasteur.fr

BIMET François, engineer,fbimet@pasteur.fr,

CLERMONT Dominique, engineer,dclermon@pasteur.fr

  Carole TCHOKOTEU

BREMONT Sylvie, technician,sbremont@pasteur.fr,

CANDREA Adina, technician,acandrea@pasteur.fr,

FEURER Carole, technician,

GRANDET Patricia, technician,pgrandet@pasteur.fr,

HAMON Sylviane, technician,shamon@pasteur.fr,

OUSSARGHIN Nadia,

RIVERA MELLADO Téo,trivera@pasteur.fr,

VIVIER Catherine, technician,cvivier@pasteur.fr,

ZOQUE Gustave

COUTELLIER Sabrina, secretary,scoutell@pasteur.fr,

ROPARS Sandrine, secretary,sropars@pasteur.fr


Activity Reports 2002 - Institut Pasteur
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