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  Ptgen


  Director : Christiane Bouchier (bouchier@pasteur.fr)


  abstract

 

Genomic platform realize large-scale projects with several teams of Pasteur Institute as part of Genopole programm on Microbiology initiated by the Ministry of Education and Research. Genomic projects have been selected by a steering committee. These projects have been realized following a automation-based protocol providing a high-thoughput low-cost method for the isolation and purification of plasmid template samples for use on capillary-based DNA sequencing devices. Genomic platform have 5 capillary sequencers (ABI model) : 4 ABI Prism 3700 (96 capillaries) and one ABI Prism 3100 (16 capillaries).



  report

cale

List of selected projects by the Steering committee (September 15th 2000)

Projects realized in 2001

Responsable

Number of purification of cloned DNA

Number of sequences

Partial sequencing of Candida glabrata (2,5 X genome coverage)

B. Dujon

34848

67392

Tuberculosis complex

K. Eiglmeier -T. Garnier

9792

19584

EST sequencing of Aspergillus nidulans

C. d'Enfert

4512

9024

Molecular evolution of snake venom enzymes

V. Choumet

2112

7488

Partial sequencing of Mycobacterium ulcerans

T. Stinear

27840

55680

PCR products sequencing - ORF of Helicobacter pylori

A. Labigne

Purification of PCR products

1827

 

Projects in progress

Responsable

Number of purification of cloned DNA

Number of sequences

Partial sequencing of Candida glabrata 3 X

B. Dujon

11712

23424

Partial sequencing of Microcystis aeruginosa

N. Tandeau de Marcac

3522

6467

Antibiotic multiresistance of M. tuberculosis strains

B. Gicquel

Purification of PCR products

192

Allelic diversity of Plasmodium falciparum

O. Puijalon

Purification of PCR products

2016

Genetic variability of influenza virus

S. van der Werf

Purification of PCR products

176

EST sequencing of Entamoeba histolytica

N. Guillen

960

1824


High-throughput DNA preparation and sequencing reactions



  publications

puce Publications of the unit on Pasteur's references database


  personnel

  Office staff Researchers Scientific trainees Other personnel
       

Stéphanie DUTHOY IP- technician

Stéphane FERRIS IP technician

Laurence MA IP technician

Eliana OCHOA IP technician

Magali TICHIT IP technician


filet

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