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  Cytoanal


  Director : MÉTÉZEAU philippe (metezeau@pasteur.fr)


  abstract

 

The laboratory makes flow cytometry analysis and sorting. It organizes learning and development of methodologies and technologies in the field of cell analysis and sorting.
key words : flow cytometry, confocal microscopy, cell sorting, celle analysis, image cytometry, fluorescence.



  report

cale

The laboratory is primarly useful for experiments in the field of flow and image cytometry.

The principal activities are cell analysis and sorting. We use a Laser scanning image cytometer, a confocal microscope, three cell-sorters, and four flow cytometrer-analysers. Particularly, this year, we have obtained a very rapid cell sorter (25 000 cell analyzed and sorted /sec.) ; it is a good example of second generation of practical sorter. Lasers which equiped our machines allow visible and UV excitations of many fluorochromes. The most demanding applications are immunolfluorescent detections, cell cycle analysis., apoptosis and pH measurements (5000 analysis and 250 sortings by year). Moreover, the laboratory organizes learning (85 students)



  web site

puce More informations on our web site


  publications

puce Publications of the unit on Pasteur's references database


  personnel

  Office staff Researchers Scientific trainees Other personnel
       

MÉTÉZEAU Philippe, IP

BALLAZUC, Anne-Marie, IP

LOUISE Anne, IP

KIEFER-BIASIZZO Hélène, IP

WAGNER Marie-Christine, IP


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