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  Collec


  Director : Chantal Bizet (bizet@pasteur.fr)


  abstract

 

The role of the Collection de l'Institut Pasteur (CIP) is to ensure the maintenance and enrichment of its bacterial collection, the production of a catalogue (the 7th edition was published in 1998), the diffusion of information on the distributed strains and finally the development of research on identification, taxonomy and preservation of bacteria. The CIP is the first French ISO 9002 certificated collection.



  report

cale

Strain stock management (C. Bizet)

7.000 strains are available on stock (1.850 different species). Every year, new bacterial strains are added to the collection coming either from researchers of the Institut Pasteur, or foreign collections or newly deposited strains after having connection with foreign or French researchers. We've got also plasmids, transposons, host strains, probes. Titrated spore suspensions (species Bacillus and Clostridium) are available. Strains are preserved in two different places and by two different ways (freeze dried and deep frozen at - 80°C, or deep frozen in nitrogen liquid and at - 80°C for strains which can't be freeze dried).


Computer system (F. Bimet)

During the whole year, an update of the database is made regularly in order to enter strain biochemical properties, information regarding the catalogue and the stock. The catalogue can be viewed on the Institut Pasteur Web site at : http://www.pasteur.fr/CIP


Studies (C. Bizet)

Studies are made according to industrial laboratory requests, regarding bacterial identification by the study of the cellwall components and protein electrophoresis and also by the study of the strains susceptibility to different antibiotics.
CIP organizes quality controls for public or private laboratories.
Bacterial strains from external collections are preserved in the CIP. It's submitted to a maintenance contract.


International relationships (C. Bizet)

CIP is a member of different official organizations : l'ECCO (European Culture Collections' Organization), CABRI (Common Access to Biotechnological Resources and Information) and WFCC (World Federation for Culture Collection).


Quality System (D. Clermont)

Process regarding activities and tasks are written in an organized process system which suits to the CIP organization. Tasks are recorded. Implemented process are always followed up and improved. Regularly, internal audits are made in order to check and measure differences between what is written and what is done.Quality indices are provided with laboratory problems and requests from customers. If necessary, corrective actions are undertaken. Certification (ISO 9002) has been granted for 3 years. But, every year, a follow up audit must be made in order to keep it and it was made by AFAQ in March 2000.
Since 2000, a new Quality Manual has been written and new processes have been implemented so that the certification renewal will take place in 2001 according to the standard ISO 9001 version 2000.


Creation of a ribotype database (D. Clermont)

Ribotyping is made with an automatic appliance called Riboprinter in order to create a database.


Re-identification of bacterial strains after description of new species (D. Clermont and F. Bimet)

Newly taxonomic studies showed a mistake in Shewanella putrefaciens strain identification with creation of new species such as Shewanella baltica, Shewanella algae and Shewanella oneidensis. Thanks to that, 12 deposited strains in the Institut Pasteur Collection between 1959 and 1992 identified as S. putrefaciens have been studied by ribotyping, substrat assimilation tests and total protein electrophoresis. Moreover, 119 Bacillus subtilis strains have been studied by ribotyping and total protein electrophoresis in order to identify 2 subspecies described recently : subtilis and spizizeni.


Spore sale (D. Clermont)

Spores of 6 different strains are available as European sporicid standards. Spore antiseptic activity is validated after having preserved them at + 4°C and at - 80°C. It's done thanks to a collaboration with MICRAAM laboratory of Marseille.


Total protein electrophoresis and chimiotaxonomy (F. Bimet)

This laboratory helps in developing bacterial identification methods by analysis of bacterial cellwall components : lipids, peptidoglycane, sugars... and total proteins. Main techniques used are chromatography and derivated techniques and also electrophoresis in discontinued gel. Results are used thanks to a computerized process with specific softwares.

We are still working on feeding several databanks with information regarding protein profiles and nature of cellwall components. This laboratory is necessary for the Collection strain check and strain expertise for industrial laboratories.

Participating to the " Coryne Group " of the S.F.M., the CIP helps in studying Corynebacterium strains whose importance in human clinical is strong.


Flow cytometry and strain preservation in collaboration with P. Lebaron CNRS-UMR7621 (Banyuls sur mer) (D. Clermont)

The validity of freeze dried bacteria was evaluated by cultural methods on solid medium and by the detection of esterase activity (ChemChrome - V6) combined with a probe staining dead cells (Dead Stain).
For Vibrio strains, viabililty assessment by physiological probes was found to be more accurate and better reproductible compared with cultural methods.



  publications

puce Publications of the unit on Pasteur's references database


  personnel

  Office staff Researchers Scientific trainees Other personnel
 

CLODION Gilberte

COUTELLIER Sabrina

ROPARS Sandrine

SHEN Lilla

BIZET Chantal

BORIE Sandrine

LUCIANI Antoine

BIMET François

BREMONT Sylvie

CLERMONT Dominique

GRANDET Patricia

HARMANT Christine

NOWAKOWSKI Mireille

PAULOUS Sylvie

PIETFROID Alain

RIVERA-MELLADO Teofilo


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