Institut Pasteur Rapport d'activité de l'unité Biologie moléculaire du gène

INSERM U277


Responsable : KOURILSKY Philippe (kourilsk@pasteur.fr)

Résumé du rapport

Les activités de l’Unité sont très largement centrées sur l’étude des populations de lymphocytes T, NK et NKT, et de leurs ligands, dans diverses situations normales et pathologiques, (infections et cancers) chez l’homme et la souris. Le laboratoire a pour ce faire mis au point les technologies appropriées, molécules de MHC solubles et tétramériques et appareillage d’analyse de la diversité des récepteurs des cellules T.

Abstract

The work carried out in the laboratory focuses on the populations of T, NK and NKT cells and on their ligands, in normal and diseased (infection and cancer) men and mice. The laboratory has developped proper tools, such as soluble and tetrameric MHC molecules and an original set up to approach the diversity of the T cell receptor.

Texte du rapport

Interaction entre molecules du CMH de classe I et leurs recepteurs a la surface des lymphocytes T ou des cellules NK. Responsable : J.-P. ABASTADO.

Etude du répertoire des lymphocytes T. Responsables P. BOUSSO et J.-P. ABASTADO.

Sélection des cellules T . Responsable J. KANELLOPOULOS.

Ontogenie precoce du systeme immunitaire notamment des cellules B, T et NK. Responsable A. CUMANO (jusqu’au 1/4/98)

Immunopathologie. Responsable G. GACHELIN.

Immunologie antitumorale et immunologie humaine. Responsables P. KOURILSKY et J. EVEN.

Personnel de l'unité

Secrétariat de l'unité

CAPUT Viviane
Tel 331 45 68 85 45
Fax 331 45 68 85 48
email caput@pasteur.fr

Chercheurs de l'unité

ABASTADO Jean-Pierre, CNRS
CUMANO Ana, INSERM
EVEN Jos, CNRS
GACHELIN Gabriel, IP
KANELLOPOULOS Jean, INSERM
KOURILSKY Philippe, Professeur au Collège de France LONE Yu-Chun, CNRS
MOTTA Iris, INSERM
PARDIGON Nathalie, IP

Stagiaires de l'unité

APOSTOLOU Irina, Doctorante
ARSTILA Petteri, Post-doc
BOUSSO Philippe, Doctorant
CALBO Sébastien, Doctorant
CAMBIAGGI Anna, Post-Doc
DOUAGI Iyadh, préparation d'un DEA
GAPIN Laurent, Doctorant
HARADA Haruhito, Post-doc
LAOUINI Dhafer, Post-doc
LEVRAUD Jean-Pierre, Doctorant puis post-doc MEMPEL Martin, Post-Doc
RONET Catherine, Doctorant
SALCEDO Margarita, Post-Doc

Autre personnel de l'unité

CAPUT Viviane, CNRS
CASROUGE Armanda, INSERM
DALLE Sophie, Collège de France
DARCHE Sylvie, INSERM
DELARBRE Christiane, CNRS
GARCIA, Sacha, INSERM
LEMAITRE Fabrice, INSERM
LIM Annick, IP

Publications de l'unité

AMIGORENA, S., LANKAR, D., BRIKEN, V., GAPIN, L., VIGUIER, M. & BONNEROT, C.: Type II and III receptors for immunoglobulin G (IgG) control the presentation of different T cell epitopes from single IgG-complexed antigens. J. Exp. Med. (1998) 187, 505-515.

BOUSSO, P., CASROUGE, A., ALTMAN, J.D., HAURY, M., KANELLOPOULOS, J., ABASTADO, J.-P. & KOURILSKY, P.: Individual variations in the murine T cell response to a specific peptide reflect variability in naive repertoires. Immunity (1998) 9, 169-178.

DELARBRE, C., SPRUYT, N., DELMARRE, C., GALLUT, C., BARRIEL, V., JANVIER, P., LAUDET, V. & GACHELIN, G.: The complete nucleotide sequence of the mitochondrial DNA of the dogfish, Scyliorhinus canicula.. Genetics (1998) 150, 331-344.

DELASSUS, S., DARCHE, S., KOURILSKY, P. & CUMANO, A.: Ontogeny of the heavy-chain immunoglobulin repertoire in fetal liver and bone marrow. J. Immunol. (1998) 160, 3274-3280

DELON, J., GREGOIRE, C., MALISSEN, B., DARCHE, S., LEMAITRE, F., KOURILSKY, P., ABASTADO, J.-P. & TRAUTMANN, A.: CD8 expression allows T cell signalling by monomeric peptide-MHC complexes. Immunity (1998) 9, 467-473.

DOUILLARD, P., JOSIEN, P., PANNETIER, C., BONNEVILLE, M., SOULILLOU, J.-P. & CUTURI, M.-C.: Selection of T cell clones with restricted TCR-CDR3 lengths during in vitro and in vivo alloresponses. International Immunology (1998) 10, 71-83.

EMA, H., DOUAGI, I., CUMANO, A. & KOURILSKY, P.: Development of T cell precursor activity in the murine fetal liver. Eur. J. Immunol. (1998) 28, 1563-1569.

FAURE, F., EVEN, J. & KOURILSKY, P.: Tumor-specific immune response: current in vitro analyses may not reflect the in vivo immune status. Critical Reviews in Immunology (1998) 18, 77-86.

GAPIN, L., BRAVO DE ALBA, Y., CASROUGE, A., CABANIOLS J.-P., KOURILSKY, P. & KANELLOPOULOS, J.: Antigen presentation by dendritic cells focuses T-cell responses against immunodominant peptides: studies in the hen egg-white lyzosyme (HEL) model. J. Immunol. (1998) 160: 1555-1564.

GAPIN L., FUKUI Y., KANELLOPOULOS J., SANO T., CASROUGE A., MALIER V., BEAUDOING E., GAUTHERET D., CLAVERIE J.-M., SASAZUKI T. & KOURILSKY P.: Quantitative analysis of the T cell repertoire selected by a single peptide/Major Histocompatibility Complex J. Exp. Med. (1998) 187, 1871-1883.

GARDERET, L., DULPHY, N., DOUAY, C., CHALUMEAU, N., SCHAEFFER, V., ZILBER, M.-T., LIM, A., EVEN, J., MOONEY, N., GELIN, C., GLUCKMAN, E., CHARRON, D. & TOUBERT, A.: The umbilical cord blood alpah,beta T-cell repertoire: characteristics of a polyclonal and naive but completely formed repertoire . Blood (1998) 91, 340-346.

GASCHET, J., GALLOT, G., IBISCH, C., LIM, A., EVEN, J., VIVIEN, R., HALLET, M.M., MILPIED, N. & VIE, H.: Acute graft-versus-host disease after bone marrow transplantation with a signle HLA-DPB1*1001 mismatch: involvement of different TCRBV subsets. Bone Marrow Transplantation (1998) 22, 385-392.

KOURILSKY, P., BOUSSO, P., CALBO, S. & GAPIN, L.: Immunological issues in vaccine trials: T-cell responses. In: "Preclinical and Clinical Development of New Vaccines", Eds.: F. Brown and S. Plotkin. - Dev. Biol. Stand., Basel, Karger (1998) vol. 95, pp. 117-124.

KOURILSKY, P., BOUSSO, P., CALBO, S., GAPIN, L. & LEVRAUD, J.-P.: T cells and vaccination. Res. Immunol. (1998) 149, 41-43.

KOURILSKY, P. & GAPIN, L.: Genetic analysis at the molecular level. in: Encyclopedia of Immunology (Second Version) Academic Press (1998) pp. 981-986. LEVRAUD J.P.: Some new techniques for RNA or DNA analysis of interest for the immunologits. Res. Immunol. (1998) 149, 119-125.

LONE, Y.-C., MOTTA, I., MOTTEZ, E., GUILLOUX, Y., LIM, A., DEMAY, F., LEVRAUD, J.P., KOURILSKY, P. & ABASTADO, J.P.: In vitro induction of specific cytotoxic T lymphocytes using recombinant single-chain MHC class I/peptide complexes. J. Immunoth. (1998) 21, 283-294.

MAHIOU, J., ABASTADO, J.-P., CABANIE, L. & GODEAU, F.: Soluble FasR ligand-binding domain: high yield production of active fusion and non-fusion recombinant proteins using the baculovirus/insect cell system. Biochem. J. (1998) 330, 1051-1058.

MATSUMOTO, N., RIBAUDO, R.K., ABASTADO, J.-P., MARGULIES, D.H. & YOKOYAMA, W.M.: The lectin-like NK cell receptor Ly-49A recognizes a carbohydrate-independent epitope on its MHC class I ligand. Immunity (1988) 8, 245-254.

MOTTA, I., LONE, Y.-C. & KOURILSKY, P.: In vitro induction of naive cytotoxic T lympocytes with complexes of peptide and recombinant MHC class I molecules coated onto beads: role of TCR/ligand density. Eur. J. Immunol. (1998) 28, 3685-3695.

MÜSCHEN, M., WARSKULAT, U., HÄUSSINGER, D., MOERS, C., SIMON, D. & EVEN, J.: Deranged CD95 system in a case of Churg-Strauss vasculitis. Gastroenterology (1998) 114, 1351-1352.

MUSETTE, P.: T cells in systemic Lupus erythematosus. Dermatology (1998) 196, 281-282.

NEUBAUER, H., CUMANO, A., MÜLLER, M., WU H., HUFFSTADT, U. & PFEFFER, K.: Jak2 deficiency defines an essential developmental checkpoint in definitive hemotpoiesis. Cell (1998) 93, 397-409.

NOUN, G., REBOUL, M., ABASTADO, J.-P., KOURILSKY, P.: SIGAUX, F. & PLA, M.: Strong alloantigenicity of the alpha-helices residues of the MHC class I molecule. J. Immunol. (1998) 161, 148-153.

NOURRIT, F., DOYEN, N., KOURILSKY, P., ROUGEON, F. & CUMANO, A.: Extensive junctional diversity of Ig heavy chain rearrangements generated in the progeny of single fetal multipotent hematopoietic cells in the absence of selection. J. Immunol. (1998) 160, 4254-4261.

OJCIUS, D.M., BRAVO DE ALBA, Y., KANELLOPOULOS, J.M., HAWKINS, R.A., KELLY, K.A., RANK, R.G. & DAUTRY-VARSAT: Internalization of Chlamydia by dendritic cells and stimulation of Chlamydia-specific T cells. J. Immunol. (1998) 160, 1297-1303.

PARDIGON, N., BERCOVICI, N., CALBO, S., SANTOS-LIMA, E.C., LIBLAU, R., KOURILSKY, P. & ABASTADO, J.-P.: Role of costimulation in CD8+ T cell activation. International Immunology (1998) 10, 619-630.

SALCEDO, M., BOUSSO, P., LJUNGGREN H.-G., KOURILSKY, P. & ABASTADO, J.-P.: The Qa-1b molecule binds to a large subpopulation of murine NK cells. Eur. J. Immunol. (1998) 28, 4356-4361.

SOURDIVE, D.J.D., MURALI-KRISHNA, K., ALTMAN, J.D., ZAJAC, A.J., WHITMIRE, J.K., PANNETIER, C., KOURILSKY, P., EVAVOLD, B., SETTE, A. & AHMED, R.: Conserved T cell receptor repertoire in primary and memory CD8 T cell responses to an acute viral infection. J. Exp. Med. (1998) 188, 71-82.

SPRUYT, N., DELARBRE, C., GACHELIN, G. & LAUDET, V.: Complete sequence of the amphioxus (Branchiostoma lanceolatum) mitochondiral génome: relations to vertebrates. Nucl. Acids Res. (1998) 26, 3279-3285.

YOTNDA, P., FIRAT, H., GARCIA-PONS, F., GARCIA, Z., GOURRU, G., VERNANT, J.-P., LEMONNIER, F.A., LEBLOND, V. & LANGLADE-DEMOYEN, P.: Cytotoxic T cell response against the chimeric p210 BCR-ABL protein in patients with chronic myelogenous leukemia. J. Clin. Inverst. (1998) 101: 2290-2296.

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