OKP beta-lactamase amino-acid variation:

Accession(s) number(s)

OKP enzyme

pI (Ref)

3

4

9

10

13

18

19

24

29

30

31

34

43

48

49

50

51

52

55

59

70

86

92

94

129

137

146

181

184

188

192

193

194

197

202

211

229

248

256

263

265

266

267

275

Reference(s)

(References)

OKP-A-1

7.8

Y

V

I

S

A

A

V

P

I

T

R

S

V

V

S

G

R

T

A

N

V

Q

R

R

S

S

A

S

A

K

S

H

T

A

Q

Q

E

N

V

P

S

M

A

R

1, 2

AM051138 (1), AJ635401* (2)

OKP-A-2

8.0 (1)

F

Q

V

A

S

D

T

1, 2

AM051139 (1), AJ635404* (2)

OKP-A-3

7.1

A

S

A

D

D

T

K

1

AM051140, AM051141

OKP-A-4

8.0

F

A

S

D

T

1

AM051142

OKP-A-5

7.1

Q

L

A

S

R

A

D

D

T

1

AM051143

OKP-A-6

8.3

A

Q

V

A

S

R

R

A

D

D

T

K

1

AM051144

OKP-A-7

7.1

C

Q

L

A

S

R

P

A

D

D

A

K

1

AM051145, AM051146

OKP-A-8

8.1

V

A

S

D

T

1

AM051147

OKP-A-9

7.1

A

S

A

A

D

D

T

K

1

AM051148

OKP-A-10

7.1

C

P

L

A

S

A

D

D

M

T

K

1

AM051149

OKP-A-11

7.8

A

V

A

S

A

D

T

6

AM850915

OKP-A-12

7.8

A

Q

V

A

S

A

D

T

6

AM850916

OKP-A-13

nd

I

F

A

S

A

D

7

FJ534513

OKP-A-14

nd

I

F

A

S

T

D

T

I

7

FJ534512

OKP-A-15

nd

F

del

del

del

del

del

S

D

T

7

FJ755841

OKP-A-16

nd

V

A

Q

V

A

S

A

D

T

7

FJ755840

OKP-B-1

7.1 (1)

Q

K

I

G

A

M

S

H

T

T

P

S

R

D

D

A

T

G

1, 2

AM051150 (1), AJ635402* (2)

OKP-B-2

7.1

Q

K

I

G

A

S

H

T

T

P

S

R

D

D

A

T

G

1

AM051151

OKP-B-3

7.1

Q

K

I

A

S

H

I

T

T

P

S

R

D

D

A

T

V

G

1

AM051152

OKP-B-4

7.1

Q

K

I

A

S

H

T

T

P

S

R

D

D

T

V

G

1

AM051153, AM051154, AM051155

OKP-B-5

7.1 (3)

Q

K

I

A

S

H

T

I

T

T

P

S

R

D

D

T

V

G

3

AY512506

OKP-B-6

7.1 (3)

Q

K

I

A

S

H

T

T

P

S

R

D

D

A

T

G

3

AY850171

OKP-B-7

7.1

Q

K

I

A

S

H

I

T

T

P

S

R

D

D

A

T

G

1

AM051156

OKP-B-8

7.1

Q

K

I

A

S

H

T

I

T

T

P

S

R

D

D

A

T

G

1

AM051157, AM051158

OKP-B-9

7.1

Q

K

I

I

A

S

H

T

R

T

P

S

R

D

D

A

T

V

G

1

AM051159

OKP-B-10

7.1

Q

K

I

A

S

H

T

I

N

T

T

P

S

R

D

D

A

T

V

1

AM051160

OKP-B-11

7.1

T

Q

K

I

A

T

S

H

I

T

T

P

S

R

D

D

T

V

G

1

AM051161

OKP-B-12

6.5

?

?

?

T

Q

K

I

G

A

M

S

H

T

T

T

P

S

D

D

A

T

G

2

AJ635420*

OKP-B-13

nd

I

Q

K

I

A

S

H

T

I

T

T

P

S

R

D

D

A

T

V

4

AY825330

OKP-B-14

nd

?

?

Q

K

I

A

S

H

T

T

P

S

L

R

D

D

A

T

G

5

OKP-B-15

7.3

Q

K

I

A

S

H

T

T

P

S

R

D

D

A

T

V

G

6

AM850917

OKP-B-16

7.3

Q

K

I

A

S

H

T

I

T

T

P

S

R

D

D

T

G

6

AM850918

OKP-B-17

7.4

Q

K

I

G

A

S

H

T

T

P

S

R

D

D

T

V

G

6

AM850919

OKP-B-18

6.8

Q

K

I

G

A

S

H

I

T

T

P

S

D

D

A

T

G

6

AM850920

OKP-B-19

7.0

Q

K

I

G

A

M

S

H

T

I

T

T

P

S

R

D

D

T

V

G

6

AM850921

OKP-B-20

7.1

Q

K

I

A

S

H

T

I

T

T

P

S

R

D

D

A

T

V

G

6

AM850922

?: Not sequenced (total length = 286 amino-acids)

* partial sequences

del: Deletion

1 - Fevre C., Passet V., Weil F.X., Grimont P.A.D., Brisse S. Unpublished. Variants of the Klebsiella pneumoniae OKP chromosomal beta-lactamase are didivided into two main groups, OKP-A and OKP-B.

2 - Haeggman, S., S. Lofdahl, A. Paauw, J. Verhoef, and S. Brisse. 2004. Diversity and evolution of the class A chromosomal beta-lactamase gene in Klebsiella pneumoniae. Antimicrob Agents Chemother 48:2400-8.

3 - Siebor, E., A. Pechinot, J. M. Duez, and C. Neuwirth. 2005. One new LEN enzyme and two new OKP enzymes in Klebsiella pneumoniae clinical isolates and proposed nomenclature for chromosomal beta-lactamases of this species. Antimicrob Agents Chemother 49:3097-8.

4 - Wang,S.Y., He,L., Zhang,R.Z., Lu,Y.M., Mu,X.K. and Chen,S.W., unpublished

5 - M. Nogueira, submitted (6 amino-acids are not sequenced on each side of the sequence)

6 - Mendonca N, Ferreira E, Canica M. 2009. Genetic diversity of genes encoding OKP and LEN beta-lactamases produced by clinical Klebsiella pneumoniae strains in Portugal. Diagn Microbiol Infect Dis. 63(3):334-8.

7- Likou Zou, Sichuan University,China