DI 99/68 and 99/69
Novel antibody-based fluorescence biosensor using no additional reagents
Inventor: Hugues BEDOUELLE et al.
Description of invention :
A biosensor is a biomolecular probe used to measure the presence or concentration of biological molecules, biological structures, microorganisms, etc., by translating a biochemical interaction at the probe surface into a signal that can be measured. The design of biochemical detection systems that use antibodies as recognition elements has generated considerable interest from both the scientific and industrial communities, as the potential applications for this type of biosensor technology are very broad and include diagnostics, detection, process control, and environmental monitoring. The potentially high sensitivity of antibody-based systems makes them ideal in environments where speed and accuracy are a priority.
In the present invention, researchers at the Institut Pasteur have developed a novel biosensor based on recombinant technology, using a chemically-modified antibody fragment. In the presence of the appropriate ligand, a fluorescence signal is emitted by the biosensor and can be easily quantified by a spectrofluorometer or any other appropriate apparatus. Potential ligands include infectious agents, tumor cells, metabolites or any molecules that can be recognized by an antibody. Ligands can be measured in various biological media, including blood, urine and cerebrospinal fluid. It is believed that the coupling of this invention with fiber optic technology would permit in situ real-time quantification of blood metabolites. It would also be possible to generate microchips by fixation of multiple biosensors on a microplate to allow simultaneous quantification of a large number of ligands in a unique sample.
Key advantages of this technology include good specificity provided by the antibody fragment and a simple, potentially portable, quantification method, making it competitive with existing biosensor detection systems. The invention also lends itself to applications beyond the diagnostic and clinical monitoring fields, for example, for the detection of food additives, for the rapid monitoring of pollutants in water and other environmental samples as well as in bioremediation studies.
French patent filed March 1, 2000
Institut Pasteur seeks a development partner for a variety of fields not yet licensed
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