Meetings

Automation

  • 10th/11th January 2008 : Automation Scientific project management meeting and Automation Executive committees meeting. This meeting took place the 10 and 11 of January 2008 in Institut Pasteur (Paris, France).
    The agenda was the following:
    Thursday 10 January (9h00 - 12h30) Scientific presentations (all scientific participants) : Each partner presented their final results to the whole group and the scientific review committee together, with discussion. Each partner had 45 minutes. There was a review of the deliverables. Speakers in bold.
    • Partner 1: O. Renaud, C. Machu, S. Shorte - Institut Pasteur
    • Partner 3: H. van der Voort - Scientific Volume Imaging
    • Partner 4: D. Laksameethanasan, K. Palander, P. Engelhardt - Helsinki University of Technology
    • Partner 5: B. Chalmond, A. Trouvé, Y. Yu- CMLA ENS Cachan
    • Partner 6: M. Brown - Andor Technology
    • Scientific review committee: R. Heintzmann, M. Bertero, J. Pawley.

    Thursday 10 January (14h00 - 17h30): Demonstrations in the lab during the afternoon. For the demonstrations Pasteur, ENS, HUT and SVI showed some of the algorithms/software: equipment in development and launched some real-time acquisitions/calculations (using lab facilities, server and/or local or remote machines).
    Friday 11 January (9h00 - 12h30): Scientific committee deliberated and delivered a brief report on their experience and opinion on the project.
    Friday 11 January (14h00 - 17h30): Project management meeting (all management personnel plus selected scientific participants): The project closure was discussed.
  • From 12 to 13th of June 2007 : Hands on meeting at the Institut Pasteur with Bernard Chalmond (ENS Cachan), Yong Yu (ENS Cachan), Christophe Machu (IP) and Olivier Renaud (IP). The goal of this informal meeting was to show to Bernard Chalmond’s team the microrotation system under experimental conditions.
  • From 29 to 30th of March 2007 : The OME Consortium held a User’s Meeting hosted by the Plateforme d’Imagerie Dynamique, Institut Pasteur. Jose Vina (SVI), Spencer Shorte (IP), Christophe Machu (IP), Anne Danckaert (IP) and Olivier Renaud (IP) participated to this meeting and took the opportunity to discuss image management under the Automation project. For more information see Web site
  • From 4 to 8th of December 2006 : Collaboration project with National University Singapore (NUS). Partcipants from Institut Pasteur:
    • Dr. Olivier Renaud (micro-rotation imaging developer)
    • Dr. Anne Danckaert (image analysis and database expert)
    • Dr. Spencer Shorte (group leader)
    Visit of the lab of our collaborator at the NUS : Dr. Kevin TAN (Laboratory of Molecular and Cellular Parasitology, Department of Microbiology) and Dr. CT Lim groups (Nano Biomechanics Lab, Faculty of Engineering). The object of the visit was: 1) Present our unique micro-rotation (MR) imaging technologies 2) Engage pilot MR-imaging experiments 3) Advise on high-content analysis of red-blood-cell image assay (3) and present analysis strategy developed in our group 5) Examine joint resources for programming and data-sharing around this project Project developped under the MERLION programme
  • 23th/24th November 2006 : Automation Scientific project management meeting 2 and Automation Executive committees meeting 2. Meetings took place the 23 and 24 of november 2006 in Institut Pasteur (Paris, France). This meeting joined together all the partners of the automation project: Speakers in bold.
    • Partner 1: S. Shorte, A. Danckaert, C. Machu, O. Renaud - Institut Pasteur
    • Partner 2: T. Schnelle, T. Mueller - Evotec Technologies
    • Partner 3: J. Vina - Scientific Volume Imaging
    • Partner 4: S. Brandt, J. Heikkonen - Helsinki University of Technology
    • Partner 5: B. Chalmond, J. Wang, A. Trouvé, Y. Yu- CMLA ENS Cachan
    • Partner 6: M. Brown, S. Dubroecq, A. Hubbard- Andor Technology
    • and also the scientific committee: R. Heintzmann, M. Bertero, J. Pawley.

    Thursday session was open to all members of participant work-groups- to present Scientific progress report/presentation to external scientific advisory (ESA) panel ; work progress review, brainstorm and contingency planning. Friday morning session was dedicated to ESA presenting brief written report in their views concerning scientific progress to Executive Partner Committee. In the afternoon the Intellectual Property Advisory board and partners discussed potential « declaration of invention » arising from Automation results to-date.
  • 19th June 2006 : Automation Meeting Millestone 2.
    The Automation meeting took place the 19 of june 2006 in Institut Pasteur (Paris, France). All partners were present to show and discuss the new development they made:
    Speakers in bold.
    • Partner 1: S. Shorte, M. Marchand, O. Renaud- Institut Pasteur
    • Partner 2: T. Schnelle- Evotec Technologies
    • Partner 3: H. van der Voort- Scientific Volume Imaging
    • Partner 4: S. Brandt, J. Heikkonen, D. Laksameethanasan- Helsinki University of Technology
    • Partner 5: B. Chalmond, J. Wang, A. Trouvé, Y. Yu- CMLA ENS Cachan
    • Partner 6: C. Coates- Andor Technology
    The morning session was dedicated to the presentation of the different imaging modalities that can be achieved using single cell microrotation and manipulation. The afternoon session was more focused along the development of mathematical solution for 3D reconstruction of microrotation dataset.
  • 24th/25th November 2005 : Automation Meeting Millestone 1.
    The automation meeting took place the 24 and 25 of november 2005 in Institut Pasteur (Paris, France). This meeting joined together all the partners of the automation project:
    Speakers in bold.
    • Partner 1: S. Shorte, M. Marchand, L. Sengmanivong, O. Renaud - Institut Pasteur
    • Partner 2: T. Schnelle - Evotec Technologies
    • Partner 3: H. van der Voort - Scientific Volume Imaging
    • Partner 4: S. Brandt - Helsinki University of Technology
    • Partner 5: B. Chalmond, J. Wang - CMLA ENS Cachan
    • Partner 6: S. Dubroecq, A. Hubbard, D. Stacey - Andor Technology
    and also the scientific committee: R. Heintzmann, M. Bertero, J. Pawley.
    This meeting was the opportunity for all partners to discuss the last results that have been made during the last 6 months. The goal of the meeting was discuss and find a consensus how the generated dataset will be processed and exchange between all partners. Moreover, the scientific committee give us an evaluation of the scientific work that have been made and some suggestions on the future directions that the project should follow.

  • July 2005: Meeting between Spencer Shorte (Director of Imagopole and coordinator of the automation project in Instistut Pasteur) and the executive management of Evotec Technologies at the head of Evotec Technologies in Hamburg (Germany).
    The goal of this meeting was to discuss possible new development of the cell trapping system (Cytocon 400) and to develop new collaborations. This meeting was profitable for future planning and the relationship between Institut Pasteur and Evotec Technologies.
  • From 8 to 10th of June 2005: Intensive training on the cell sorting and cell trapping system from Evotec Technologies in Berlin (Germany).
    Participants : Olivier Renaud (Institut Pasteur), Thomas Schnelle (Evotec Technologies), Torsten Muller (Evotec Technologies) .
    The goal of the 3 day intensive training was to learn to gain expertise with the cell trapping and cell sorting systems. Evotec Technologies developed a new piece of hardware that allow users to do single cell sorting into 96 well plates. The new device was installed in Institut Pasteur during the last AUTOMATION meeting (may 2005).
  • 27th May 2005: Informal meeting with Bernard Chalmond (ENS), Jiaping Wang (ENS), Mathieu Marchand (Institut Pasteur) and Olivier Renaud (Institut Pasteur) in Ecole Normale Supérieure de Cachan (France).
    This meeting was really important for several reasons:
    • to develop strong relationship between the two teams that have different expertise. The team of Bernard Chalmond is mainly interested in the mathematical algorithm for 3D reconstruction whereas the team at Institut Pasteur is much more involved in observation of biological specimens using light microscopy.
    • to discuss the informatic needs: computer power, storage, file exchange etc...
    • to plan dataset acquisition of live cells using the cell rotator and also the possibility of generating confocal datasets.
  • 12th/13th May 2005 : The First Automation’s Meeting 12th and 13th May 2005
    The first automation meeting took place the 12 and 13 of may 2005 in Institut Pasteur (Paris, France). This meeting joined together all the partners of the automation project:
    Speakers in bold.
    • Partner 1: S. Shorte, M. Marchand, A. Mattheyses, L. Sengmanivong, O. Renaud - Institut Pasteur
    • Partner 2: T. Schnelle, T. Mueller, R. Guenther - Evotec Technologies
    • Partner 3: J. Vina - Scientific Volume Imaging
    • Partner 4: S. Brandt, P. Engelhardt, J. Heikkonen - Helsinki University of Technology
    • Partner 5: B. Chalmond, J. Wang - CMLA ENS Cachan
    • Partner 6: M. Browne - Andor Technology
  • This meeting was the opportunity for all partners to meet since the official launch of the automation project. The goal of the meeting was to determine a short and long term plan concerning the development of a new prototype for cell rotation and also to discuss the strategy for algorithm development to be able to make 3D reconstruction from microrotation data. Developing communication between each partner was an important point of the meeting. The project is supported by the FP6 of the European Community.

  • 18th March 2005 : Visit of Prof. Jim Pawley
    Prof. Jim Pawley (University of Wisconsin) came for a visit to the Plateforme d’Imagerie Dynamique of the Institut Pasteur. His visit was an opportunity to discuss the best way to image microrotating cells (Automation project). Prof. Pawley is one of the members of the external Scientific Advisory for the automation project. His international expertise in microscopy development and imaging will be greatly appreciated

Conferences

  • From 17th to 20th of April 2007 : ELMI Meeting on Advanced Light Microscopy in York (UK). Spencer Shorte presented our work on an oral presentation the Wednesday, 18th April at 12h30 during the session ”Live cell imaging” (session 2). ELMI web site
  • From 10 to 13th of April 2007 : Focus On Microscopy conference in Valencia (Spain). Spencer Shorte presented our work on an oral presentation the Thursday 12 of April during the session "Image processing and analysis III, biological applications, methods" (Auditorium 6). Focus On Microscopy web site.
  • On Tuesday 23th of January 2007 : Oral presentation at the SPIE symposium on Biomedical Optics (BIOS) 2007 (San Jose, USA) at the conference 6441 "Imaging, Manipulation and Analysis of Biomolecules, cells, and Tissues", session 4 "Cytomics II. A system and methodology for high-content visual screening of individual intact living cells in suspension by Dr Olivier Renaud. Proceeding
  • Tuesday 5th december 2006 : oral presentation at the National University of Singapore (invited by Dr. CT Lim). Introduction : Dr Spencer Shorte Micro-rotation tomography: A new high-content imaging modality providing enhanced 3D-resolution for live cell studies of fluorescence.
    Dr Olivier Renaud
    Abstract
  • April 2005 Second International Symposium on EMCCD Technology
    Micro-Rotational three dimensional fluorescence imaging of individuals non-adherent living cells.
    Dr Olivier Renaud
    Presentation
    EMCCD’s Web site
  • September 2003 First International Symposium on EMCCD Technology
    A new innovation in multi-dimensional live cell microscopy: Cell Rotation Imaging. Dr Spencer Shorte
    Abstract
    EMCCD’s Web site