Equipe PFID

Dr Nathalie Aulner

Areas of interest/expertise

High Content Screening, High Throughput Screening (chemical compounds and siRNA libraries), High Content Image Analysis and Data Management.


Degrees

  • 2000: PhD in Molecular Biology at the University Paul Sabatier (Toulouse III) lead by Dr. Emmanuel Käs
  • 1994: DEA in Molecular Biology and Microbial Genetics at INSA (Institut National des Sciences Appliquées, Toulouse)
  • 1994: Engineering Degree in Biochemistry and Biotechnology at INSA

Precedent Positions
  • 2009: Associate Research Scientist in the Center for Computational Biology and Bioinformatics at Columbia University Medical School (New York, USA)
  • 2004-2009: Associate Research Scientist in the Columbia University High throughput Center at Columbia University Medical School (New York, USA)
  • 2003-2004: Associate Research Scientist, Functional Proteomics Project at Memorial Sloan-Kettering Cancer Center (New York, USA)
  • 2002: Associate Research Scientist in the Center for Molecular Biology (ZMBH) (Heidelberg, Germany)

Research Publications


Nobile C, Rudnicka D, Hasan M, Aulner N, Porrot F, Machu C, Renaud O, Prévost MC, Hivroz C, Schwartz O, Sol-Foulon N. HIV-1 Nef inhibits ruffles, induces filopodia, and modulates migration of infected lymphocytes. J Virol. 2010 Mar;84(5):2282-93. Epub 2009 Dec 16.

Paumet F, Wesolowski J, Garcia-Diaz A, Delevoye C, Aulner N, Shuman HA, Subtil A, Rothman JE. Intracellular bacteria encode inhibitory SNARE-like proteins. PLoS One. 2009 Oct 12;4(10):e7375.

Wyler MR, Smith DH, Cayanis E, Többen U, Aulner N, Mayer T. Cell-based assays to probe the ERK MAP kinase pathway in endothelial cells. Methods Mol Biol.2009;486:29-41.

Rinderspacher A, Cremona ML, Liu Y, Deng SX, Xie Y, Gong G, Aulner N, Többen U, Myers K, Chung C, Andersen M, Vidovic D, Schürer S, Brandén L, Yamamoto A,
Landry DW. Potent inhibitors of Huntingtin protein aggregation in a cell-based assay. Bioorg Med Chem Lett. 2009 Mar 15;19(6):1715-7.

Gong G, Xie Y, Liu Y, Rinderspacher A, Deng SX, Feng Y, Zhu Z, Tang Y, Wyler M, Aulner N, Többen U, Smith DH, Branden L, Chung C, Schürer S, Vidovi? D,
Landry DW. Discovery of novel small molecule cell type-specific enhancers of NF-kappaB nuclear translocation. Bioorg Med Chem Lett. 2009 Feb 15;19(4):1191-4. Epub 2008 Dec 25.

Xie Y, Deng S, Thomas CJ, Liu Y, Zhang YQ, Rinderspacher A, Huang W, Gong G Wyler M, Cayanis E, Aulner N, Többen U, Chung C, Pampou S, Southall N, Vidovic D, Schürer S, Branden L, Davis RE, Staudt LM, Inglese J, Austin CP, Landry DW, Smith DH, Auld DS. Identification of N-(quinolin-8-yl)benzenesulfonamides as agents capable of down-regulating NFkappaB activity within two separate high-throughput screens of NFkappaB activation. Bioorg Med Chem Lett. 2008 Jan 1;18(1):329-35.

Mayer T, Jagla B, Wyler MR, Kelly PD, Aulner N, Beard M, Barger G, Többen U, Smith DH, Brandén L, Rothman JE. Cell-based assays using primary endothelial cells to study multiple steps in inflammation. Methods Enzymol. 2006;414:266-83.

Jagla B, Aulner N, Kelly PD, Song D, Volchuk A, Zatorski A, Shum D, Mayer T, De Angelis DA, Ouerfelli O, Rutishauser U, Rothman JE. Sequence characteristics of functional siRNAs. RNA. 2005 Jun;11(6):864-72.

Rollins RA, Korom M, Aulner N, Martens A, Dorsett D. Drosophila nipped-B protein supports sister chromatid cohesion and opposes the stromalin/Scc3 cohesion factor to facilitate long-range activation of the cut gene. Mol Cell Biol. 2004 Apr;24(8):3100-11

Monod C, Aulner N, Cuvier O, Käs E. Modification of position-effect variegation by competition for binding to Drosophila satellites. EMBO Rep. 2002 Aug;3(8):747-52.

Aulner N
, Monod C, Mandicourt G, Jullien D, Cuvier O, Sall A, Janssen S, Laemmli UK, Käs E. The AT-hook protein D1 is essential for Drosophila melanogaster development and is implicated in position-effect variegation. Mol Cell Biol. 2002 Feb;22(4):1218-32

Beaujean N, Bouniol-Baly C, Monod C, Kissa K, Jullien D, Aulner N, Amirand C, Debey P, Käs E. Induction of early transcription in one-cell mouse embryos by microinjection of the nonhistone chromosomal protein HMG-I. Dev Biol. 2000 May 15;221(2):337-54.

Benoit B, Nemeth A, Aulner N, Kühn U, Simonelig M, Wahle E, Bourbon HM. The Drosophila poly(A)-binding protein II is ubiquitous throughout Drosophila development and has the same function in mRNA polyadenylation as its bovine homolog in vitro. Nucleic Acids Res. 1999 Oct 1;27(19):3771-8.

Book Chapters

Jagla B and Aulner N. RNAi reagent design. In RNAi. Martin Latterich (Editor).Taylor & Francis Group, New York, Abingdon (2008), pp. 3-20.

Aulner N and Jagla B. Validation of RNAi. In RNAi. Martin Latterich (Editor).Taylor & Francis Group, New York, Abingdon (2008), pp.32-45.