Equipe PFID

Dr Brigitte David-Watine

Areas of interest/expertise:
  • Molecular biology (regulation of gene expression, RNA fish, mRNA export), Cell biology (cell cycle, senescence, the Tpr protein and the nuclear pore, RNA interference, regulation of SUMOylation and ubiquitination, regulation of p53), Biochemistry.

Education
  • PhD in Veterinarian Sciences - Ecole Nationale Vétérinaire d'Alfort (ENVA) - Creteil School of the Medecine-Paris XIII University, 1983.
  • PhD in Microbiology , Paris VII University, 1990.
  • Certificats d'Etudes Spéciales, Paris VI University - School of Medecine : Immunologie générale et Bactériologie et Virologie cliniques, 1982-1983.
  • Maîtrise de Biochimie Paris VII University, 1983-1984.
  • Diplôme d'Immunologie Approfondie, Institut Pasteur, 1984.
  • EMBO course on DNA-proteins interactions, EMBL, Heidelberg, 1986.

Research experience and academic positions
  • 2009-2012 Head of a E3 group-Biologie cellulaire du Noyau in URA CNRS 2582 lead by A. Israël.
  • 2001- 2009 Staff scientist in the laboratory led by Ulf Nehrbass (Biologie Cellulaire du Noyau) Pasteur Institute - acting administrative manager 2007-2009.
  • 1995- 2000 Staff scientist in the laboratory led by Prof. Henri Korn (Neurobiologie cellulaire).
  • 1991-1994 : Post-doctoral Research scientist in the laboratory led by Prof. M. Yaniv Unité des Virus Oncogènes, Pasteur Institute.
  • 1985-1990 : PhD Research Fellow in the laboratory led by Prof. P. Kourilsky (Biologie Moléculaire du Gène) Pasteur Institute ; Appointed research scientist at INSERM (CR2).
  • 1983-1984 : Vacations in the laboratory led by Pr J. Dausset in Laboratoire d'histocompatibilité, Saint-Louis hospital, Paris.
  • 1981-1982 - One year of veterinary practise.

Research publications
  • David-Watine B. Silencing Nuclear Pore Protein Tpr Elicits a Senescent-Like Phenotype in Cancer Cells (2011). PLoS ONE 6: e22423
  • Kwon YJ, Genovesio A, Kim NY, Kim HC, Jung S, David-Watine B, Nehrbass U, Emans N. (2007) High-Content Classification of Nucleocytoplasmic Import or Export Inhibitors.J Biomol Screen 12:621.
  • Munter S, Enninga J, Vazquez-Martinez R, Delbarre E, David-Watine B, Nehrbass U, Shorte SL (2006) Actin polymerisation at the cytoplasmic face of eukaryotic nuclei. BMC Cell Biology 7 : 23.
  • De Saint Jan D, David-Watine B, Korn H, Bregestovski P. Activation of human alpha1 and alpha2 homomeric glycine receptors by taurine and GABA. (2001) J Physiol 535:741-55.
  • David-Watine B, Shorte SL, Fucile S, De Saint Jan D, Korn H, Bregestovski P (1999) Functional integrity of green fluorescent protein conjugated glycine receptor channels. Neuropharmacology 38 : 785-92.
  • David-Watine B. (2001) The human gephyrin (GPHN) gene: structure, chromosome localization and expression in non-neuronal cells. Gene 271:239.
  • Ardouin O, Legouis R, Fasano L, David-Watine B, Korn H, Hardelin J, Petit C (2000) Characterization of the two zebrafish orthologues of the KAL-1 gene underlying X chromosome-linked Kallmann syndrome. Mech Dev. 90:89.
  • David-Watine B & Yaniv M. Two RAREs and an overlapping CRE are involved in the transcriptional regulation in liver of the MHC Q10 class I gene. (1996) cell Death and Differentiation 3, 37-46.
  • David-Watine B, Israël A, Kourilsky P (1990) The regulation and expression of MHC class I genes. Immunol Today 11:286.
  • David-Watine B, Logeat F, Israel A, Kourilsky P (1990) Regulatory elements involved in the liver-specific expression of the mouse MHC class I Q10 gene: characterization of a new TATA-binding factor. Int Immunol. 2:981.
  • Transy C, Nash SR, David-Watine B, Cochet M, Hunt SW 3rd, Hood LE, Kourilsky P (1987) A low polymorphic mouse H-2 class I gene from the Tla complex is expressed in a broad variety of cell types. J Exp Med 166:341.
  • David-Watine B, Transy C, Gachelin G, Kourilsky P (1987) Tissue-specific expression of the mouse Q10 H-2 class-I gene during embryogenesis. Gene 61:145.
  • Tronche F, Bach I, Chouard T, David-Watine B, Pontoglio M, Ringeisen F, Sourdive D, Thépot D and Yaniv M. Hepatocyte Nuclear Factor 1 (HNF1) and liver gene expression.. in Liver Gene Expression, chapitre 9, 1994, édité par F. Tronche et M.Yaniv, R.G.Landes Company, Austin.
  • David-Watine B. Expression of MHC antigens on fetal and placental cells .. in : " The Immunology of the Fetus", G. Chaouat (ed.), 1991, C.R.C. Press Inc., Boca Raton, FL 33431 USA